Rh6DG309700

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002250
Physical Location & Seq
Forward (+)
51046307 .. 51048130
1824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG309700.1

Sequence Viewer

Length: 993 bp
ATGGCCTCTCAATCTTCACCTTCATCTCCTTCTTCTTCTTCTTCTTCTTCATCTTCCTTATCATCATCATCATCATCATCTCCGCCTCTGATCCCAGGCCTCCCAAACGACGTCGCTTTCTTAATCCTCTCCTTCATCCCCTTCTCCCACCACGCCCGCCTCAAACCCACCTGCAAATCATGGCGCCTCTTCCTCTCCTCCAAAACCCTCTTCTCTTCCCGCCAAACCCACCGCAGCCTCTCCCACCTCATCTGCATCTTCCCCGAGGACCCCGCCGTTGCTGGACCCTACCTCTTCGACCCCCGCCACCTCGCCTGGTGCCCCCTCCCCCCCATGCCATGTAACCCCTACGTCTACGGCCTCTGCAACTTCACCTCCGTCGCGATCGGGCCCCACCTCTACGTCCTCGGCGGCTCTCTCTTCGACGCGCGCTCCTTCCCGATCGACCGCCCCACGCCGTCGTCGGCGGTGTTCCGGTTTGACTTCACCACCGCCGCCTGGGAGTACCGCGCTCCGATGCTCACCCCGCGCGGGAGCTTCGCGTGTGCGAAGGTTCCCGATTCCGACCAAATCATCGTCGCCGGAGGCGGTTCCCGCCACACCATGTTCAGCGCCGCCGGGAGCAGGATGAGCTCGGTGGAGAGGTATGATATATGTAGAGACGAGTGGGTGGCCATGGAAGGGCTGCCGAGCTTTCGGGCCGGGTGCGTGGGGTTCTTTGCTGGAGATGAGGAGGAGAGGGAGTTTTGGGTGATGGGCGGGTACGGCCAGGCCAGGACTATATCGGAGGTGGTGCCGGTGGACGAGTATTACCGGAATGCCGTGGTGATGGAATTGACGAGTACGAATGGCGGGGGCAGGTGGAGGGAGACTGGTGATATGTGGGAAGGAGGGGAGAGAATGAGGCTTGGGAAAATTGTGGTGGTTGACAATGAGGTTGATCCTGCACAACCTGGTGTGTTCATGCTTGATGGGAATGATATATTCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

330

Amino Acids

36.19

Weight (kDa)

5.82

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_1 PF01344 122 - 173 6.9e-06 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 179, 849
AatII GACGTC 1 cut(s) 114
Acc36I ACCTGC 2 cut(s) 179, 849
AccB1I GGYRCC 3 cut(s) 183, 318, 793
AccI GTMKAC 1 cut(s) 354
AccII CGCG 7 cut(s) 383, 428, 430, 510, 529, 531, 542
AclWI GGATC 2 cut(s) 85, 935
AcoI YGGCCR 2 cut(s) 672, 766
AcyI GRCGYC 2 cut(s) 111, 184
AfaI GTAC 3 cut(s) 506, 764, 844
AfiI CCNNNNNNNGG 4 cut(s) 498, 531, 624, 681
AjnI CCWGG 6 cut(s) 94, 314, 497, 768, 773, 952
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AluBI AGCT 3 cut(s) 537, 633, 693
AluI AGCT 3 cut(s) 537, 633, 693
Alw21I GWGCWC 1 cut(s) 635
Alw26I GTCTC 2 cut(s) 654, 863
AlwI GGATC 2 cut(s) 85, 935
Ama87I CYCGRG 1 cut(s) 263
AoxI GGCC 8 cut(s) 3, 97, 358, 389, 672, 699, 766, 771
ApaI GGGCCC 1 cut(s) 393
ApeKI GCWGC 2 cut(s) 234, 685
AspLEI GCGC 6 cut(s) 186, 430, 432, 512, 531, 614
AspS9I GGNCC 5 cut(s) 268, 284, 389, 390, 699
AsuC2I CCSGG 2 cut(s) 619, 703
AsuHPI GGTGA 7 cut(s) 9, 364, 478, 514, 763, 838, 887
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 2 cut(s) 268, 284
BaeGI GKGCMC 2 cut(s) 323, 393
BalI TGGCCA 1 cut(s) 674
BanI GGYRCC 3 cut(s) 183, 318, 793
BanII GRGCYC 2 cut(s) 393, 635
Bbv12I GWGCWC 1 cut(s) 635
BbvI GCAGC 2 cut(s) 246, 672
BccI CCATC 3 cut(s) 748, 823, 965
BceAI ACGGC 5 cut(s) 260, 373, 442, 781, 806
BciT130I CCWGG 6 cut(s) 96, 316, 499, 770, 775, 954
BcnI CCSGG 2 cut(s) 619, 703
BcoDI GTCTC 2 cut(s) 654, 863
BfoI RGCGCY 2 cut(s) 187, 615
BfuAI ACCTGC 2 cut(s) 179, 849
BisI GCNGC 5 cut(s) 235, 412, 495, 615, 686
BlsI GCNGC 5 cut(s) 236, 413, 496, 616, 687
Bme1390I CCNGG 8 cut(s) 96, 316, 499, 619, 703, 770, 775, 954
Bme18I GGWCC 2 cut(s) 268, 284
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 5 cut(s) 268, 284, 389, 390, 699
BmiI GGNNCC 9 cut(s) 185, 270, 286, 320, 391, 392, 555, 592, 795
BmrFI CCNGG 8 cut(s) 96, 316, 499, 619, 703, 770, 775, 954
BmsI GCATC 2 cut(s) 264, 507
BpmI CTGGAG 1 cut(s) 744
BpuMI CCSGG 2 cut(s) 619, 703
BsaHI GRCGYC 2 cut(s) 111, 184
BsaJI CCNNGG 6 cut(s) 94, 264, 406, 498, 675, 822
BsaWI WCCGGW 2 cut(s) 474, 813
BsaXI ACNNNNNCTCC 6 cut(s) 359, 389, 416, 446, 728, 758
Bsc4I CCNNNNNNNGG 4 cut(s) 498, 531, 624, 681
Bse118I RCCGGY 1 cut(s) 796
Bse1I ACTGG 1 cut(s) 877
BseBI CCWGG 6 cut(s) 96, 316, 499, 770, 775, 954
BseDI CCNNGG 6 cut(s) 94, 264, 406, 498, 675, 822
BseGI GGATG 2 cut(s) 135, 633
BseLI CCNNNNNNNGG 4 cut(s) 498, 531, 624, 681
BseNI ACTGG 1 cut(s) 877
BsePI GCGCGC 1 cut(s) 428
BseRI GAGGAG 3 cut(s) 187, 746, 749
BseSI GKGCMC 2 cut(s) 323, 393
BseXI GCAGC 2 cut(s) 246, 672
BsgI GTGCAG 1 cut(s) 930
Bsh1236I CGCG 7 cut(s) 383, 428, 430, 510, 529, 531, 542
Bsh1285I CGRYCG 3 cut(s) 387, 444, 448
BshFI GGCC 8 cut(s) 5, 99, 360, 391, 674, 701, 768, 773
BshNI GGYRCC 3 cut(s) 183, 318, 793
BsiEI CGRYCG 3 cut(s) 387, 444, 448
BsiHKAI GWGCWC 1 cut(s) 635
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 6 cut(s) 475, 582, 618, 702, 797, 814
BslI CCNNNNNNNGG 4 cut(s) 498, 531, 624, 681
BsmAI GTCTC 2 cut(s) 654, 863
BsmBI CGTCTC 1 cut(s) 654
BsmI GAATGC 1 cut(s) 823
BsnI GGCC 8 cut(s) 5, 99, 360, 391, 674, 701, 768, 773
BsoBI CYCGRG 1 cut(s) 263
Bsp120I GGGCCC 1 cut(s) 389
Bsp1286I GDGCHC 3 cut(s) 323, 393, 635
Bsp143I GATC 4 cut(s) 90, 384, 441, 940
Bsp19I CCATGG 1 cut(s) 675
Bsp68I TCGCGA 1 cut(s) 383
BspANI GGCC 8 cut(s) 5, 99, 360, 391, 674, 701, 768, 773
BspFNI CGCG 7 cut(s) 383, 428, 430, 510, 529, 531, 542
BspLI GGNNCC 9 cut(s) 185, 270, 286, 320, 391, 392, 555, 592, 795
BspMI ACCTGC 2 cut(s) 179, 849
BspPI GGATC 2 cut(s) 85, 935
BspT107I GGYRCC 3 cut(s) 183, 318, 793
BsrFI RCCGGY 1 cut(s) 796
BsrI ACTGG 1 cut(s) 877
BssAI RCCGGY 1 cut(s) 796
BssECI CCNNGG 6 cut(s) 94, 264, 406, 498, 675, 822
BssHII GCGCGC 1 cut(s) 428
BssMI GATC 4 cut(s) 90, 384, 441, 940
BssNI GRCGYC 2 cut(s) 111, 184
BssT1I CCWWGG 1 cut(s) 675
Bst2UI CCWGG 6 cut(s) 96, 316, 499, 770, 775, 954
Bst6I CTCTTC 5 cut(s) 194, 215, 220, 299, 425
BstACI GRCGYC 2 cut(s) 111, 184
BstC8I GCNNGC 2 cut(s) 157, 430
BstDSI CCRYGG 2 cut(s) 675, 822
BstF5I GGATG 2 cut(s) 135, 633
BstFNI CGCG 7 cut(s) 383, 428, 430, 510, 529, 531, 542
BstH2I RGCGCY 2 cut(s) 187, 615
BstHHI GCGC 6 cut(s) 186, 430, 432, 512, 531, 614
BstKTI GATC 4 cut(s) 93, 387, 444, 943
BstMAI GTCTC 2 cut(s) 654, 863
BstMBI GATC 4 cut(s) 90, 384, 441, 940
BstMCI CGRYCG 3 cut(s) 387, 444, 448
BstMWI GCNNNNNNNGC 4 cut(s) 526, 594, 630, 765
BstNI CCWGG 6 cut(s) 96, 316, 499, 770, 775, 954
BstSCI CCNGG 8 cut(s) 94, 314, 497, 617, 701, 768, 773, 952
BstSLI GKGCMC 2 cut(s) 323, 393
BstUI CGCG 7 cut(s) 383, 428, 430, 510, 529, 531, 542
BstV1I GCAGC 2 cut(s) 246, 672
BsuRI GGCC 8 cut(s) 5, 99, 360, 391, 674, 701, 768, 773
BtgI CCRYGG 2 cut(s) 675, 822
BtsCI GGATG 2 cut(s) 135, 633
BtuMI TCGCGA 1 cut(s) 383
BveI ACCTGC 2 cut(s) 179, 849
Cac8I GCNNGC 2 cut(s) 157, 430
CfoI GCGC 6 cut(s) 186, 430, 432, 512, 531, 614
Cfr10I RCCGGY 1 cut(s) 796
Cfr13I GGNCC 5 cut(s) 268, 284, 389, 390, 699
CseI GACGC 1 cut(s) 434
CsiI ACCWGGT 1 cut(s) 952
Csp6I GTAC 3 cut(s) 505, 763, 843
CviAII CATG 6 cut(s) 180, 334, 339, 604, 676, 964
CviQI GTAC 3 cut(s) 505, 763, 843
DinI GGCGCC 1 cut(s) 185
DpnI GATC 4 cut(s) 92, 386, 443, 942
DpnII GATC 4 cut(s) 90, 384, 441, 940
EaeI YGGCCR 2 cut(s) 672, 766
Eam1104I CTCTTC 5 cut(s) 194, 215, 220, 299, 425
EarI CTCTTC 5 cut(s) 194, 215, 220, 299, 425
EciI GGCGGA 1 cut(s) 72
Ecl136II GAGCTC 1 cut(s) 633
Eco130I CCWWGG 1 cut(s) 675
Eco147I AGGCCT 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 393, 635
Eco47I GGWCC 2 cut(s) 268, 284
Eco53kI GAGCTC 1 cut(s) 633
Eco88I CYCGRG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 633
EcoO109I RGGNCCY 2 cut(s) 268, 390
EcoRII CCWGG 6 cut(s) 94, 314, 497, 768, 773, 952
EcoT14I CCWWGG 1 cut(s) 675
EcoT38I GRGCYC 2 cut(s) 393, 635
EgeI GGCGCC 1 cut(s) 185
EheI GGCGCC 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 675
Esp3I CGTCTC 1 cut(s) 654
FaeI CATG 6 cut(s) 183, 337, 342, 607, 679, 967
FatI CATG 6 cut(s) 179, 333, 338, 603, 675, 963
FauI CCCGC 9 cut(s) 164, 227, 280, 311, 524, 534, 602, 752, 845
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 5 cut(s) 235, 412, 495, 615, 686
FokI GGATG 2 cut(s) 122, 640
FriOI GRGCYC 2 cut(s) 393, 635
Fsp4HI GCNGC 5 cut(s) 235, 412, 495, 615, 686
GlaI GCGC 6 cut(s) 185, 429, 431, 511, 530, 613
GluI GCNGC 5 cut(s) 235, 412, 495, 615, 686
GsuI CTGGAG 1 cut(s) 744
HaeII RGCGCY 2 cut(s) 187, 615
HaeIII GGCC 8 cut(s) 5, 99, 360, 391, 674, 701, 768, 773
HapII CCGG 6 cut(s) 475, 582, 618, 702, 797, 814
HgaI GACGC 1 cut(s) 434
HhaI GCGC 6 cut(s) 186, 430, 432, 512, 531, 614
Hin1I GRCGYC 2 cut(s) 111, 184
Hin1II CATG 6 cut(s) 183, 337, 342, 607, 679, 967
Hin6I GCGC 6 cut(s) 184, 428, 430, 510, 529, 612
HinP1I GCGC 6 cut(s) 184, 428, 430, 510, 529, 612
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HinfI GANTC 1 cut(s) 560
HpaII CCGG 6 cut(s) 475, 582, 618, 702, 797, 814
HphI GGTGA 7 cut(s) 9, 364, 478, 514, 763, 838, 887
Hpy166II GTNNAC 3 cut(s) 355, 802, 928
Hpy188I TCNGA 4 cut(s) 90, 516, 565, 787
Hpy188III TCNNGA 3 cut(s) 382, 439, 557
Hpy8I GTNNAC 3 cut(s) 355, 802, 928
Hpy99I CGWCG 7 cut(s) 113, 116, 383, 428, 463, 466, 581
HpyAV CCTTC 8 cut(s) 30, 39, 142, 151, 445, 544, 674, 881
HpyCH4IV ACGT 3 cut(s) 111, 351, 402
HpyCH4V TGCA 4 cut(s) 174, 255, 366, 947
HpyF10VI GCNNNNNNNGC 4 cut(s) 526, 594, 630, 765
HpySE526I ACGT 3 cut(s) 111, 351, 402
Hsp92I GRCGYC 2 cut(s) 111, 184
Hsp92II CATG 6 cut(s) 183, 337, 342, 607, 679, 967
HspAI GCGC 6 cut(s) 184, 428, 430, 510, 529, 612
KasI GGCGCC 1 cut(s) 183
Kzo9I GATC 4 cut(s) 90, 384, 441, 940
LmnI GCTCC 4 cut(s) 437, 517, 534, 621
Lsp1109I GCAGC 2 cut(s) 246, 672
LweI GCATC 2 cut(s) 264, 507
MabI ACCWGGT 1 cut(s) 952
MaeII ACGT 3 cut(s) 111, 351, 402
MaeIII GTNAC 1 cut(s) 341
MalI GATC 4 cut(s) 92, 386, 443, 942
MboI GATC 4 cut(s) 90, 384, 441, 940
MhlI GDGCHC 3 cut(s) 323, 393, 635
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 2 cut(s) 833, 915
MluNI TGGCCA 1 cut(s) 674
Mly113I GGCGCC 1 cut(s) 184
MmeI TCCRAC 1 cut(s) 588
Mox20I TGGCCA 1 cut(s) 674
MscI TGGCCA 1 cut(s) 674
MseI TTAA 1 cut(s) 122
Msp20I TGGCCA 1 cut(s) 674
MspI CCGG 6 cut(s) 475, 582, 618, 702, 797, 814
MspR9I CCNGG 8 cut(s) 96, 316, 499, 619, 703, 770, 775, 954
Mva1269I GAATGC 1 cut(s) 823
MvaI CCWGG 6 cut(s) 96, 316, 499, 770, 775, 954
MvnI CGCG 7 cut(s) 383, 428, 430, 510, 529, 531, 542
MwoI GCNNNNNNNGC 4 cut(s) 526, 594, 630, 765
NarI GGCGCC 1 cut(s) 184
NciI CCSGG 2 cut(s) 619, 703
NcoI CCATGG 1 cut(s) 675
NdeII GATC 4 cut(s) 90, 384, 441, 940
NlaIII CATG 6 cut(s) 183, 337, 342, 607, 679, 967
NlaIV GGNNCC 9 cut(s) 185, 270, 286, 320, 391, 392, 555, 592, 795
NmeAIII GCCGAG 2 cut(s) 387, 714
NruI TCGCGA 1 cut(s) 383
PaqCI CACCTGC 2 cut(s) 179, 849
PauI GCGCGC 1 cut(s) 428
PceI AGGCCT 1 cut(s) 99
PctI GAATGC 1 cut(s) 823
PfeI GAWTC 1 cut(s) 560
PkrI GCNGC 5 cut(s) 236, 413, 496, 616, 687
Ple19I CGATCG 2 cut(s) 387, 444
PluTI GGCGCC 1 cut(s) 187
PpuMI RGGWCCY 1 cut(s) 268
Psp124BI GAGCTC 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 268
Psp6I CCWGG 6 cut(s) 94, 314, 497, 768, 773, 952
PspGI CCWGG 6 cut(s) 94, 314, 497, 768, 773, 952
PspN4I GGNNCC 9 cut(s) 185, 270, 286, 320, 391, 392, 555, 592, 795
PspOMI GGGCCC 1 cut(s) 389
PspPI GGNCC 5 cut(s) 268, 284, 389, 390, 699
PspPPI RGGWCCY 1 cut(s) 268
PteI GCGCGC 1 cut(s) 428
PvuI CGATCG 2 cut(s) 387, 444
RruI TCGCGA 1 cut(s) 383
RsaI GTAC 3 cut(s) 506, 764, 844
RsaNI GTAC 3 cut(s) 505, 763, 843
SacI GAGCTC 1 cut(s) 635
SaqAI TTAA 1 cut(s) 122
SatI GCNGC 5 cut(s) 235, 412, 495, 615, 686
Sau3AI GATC 4 cut(s) 90, 384, 441, 940
Sau96I GGNCC 5 cut(s) 268, 284, 389, 390, 699
ScrFI CCNGG 8 cut(s) 96, 316, 499, 619, 703, 770, 775, 954
SduI GDGCHC 3 cut(s) 323, 393, 635
SexAI ACCWGGT 1 cut(s) 952
SfaNI GCATC 2 cut(s) 264, 507
SfoI GGCGCC 1 cut(s) 185
SinI GGWCC 2 cut(s) 268, 284
Sse9I AATT 2 cut(s) 833, 915
SseBI AGGCCT 1 cut(s) 99
SspDI GGCGCC 1 cut(s) 183
SstI GAGCTC 1 cut(s) 635
StuI AGGCCT 1 cut(s) 99
StyD4I CCNGG 8 cut(s) 94, 314, 497, 617, 701, 768, 773, 952
StyI CCWWGG 1 cut(s) 675
TaiI ACGT 3 cut(s) 114, 354, 405
TaqI TCGA 3 cut(s) 297, 423, 444
TasI AATT 2 cut(s) 833, 915
TauI GCSGC 3 cut(s) 414, 497, 617
TfiI GAWTC 1 cut(s) 560
Tru1I TTAA 1 cut(s) 122
Tru9I TTAA 1 cut(s) 122
TseI GCWGC 2 cut(s) 234, 685
TspDTI ATGAA 4 cut(s) 12, 39, 124, 952
TspGWI ACGGA 1 cut(s) 367
VpaK11BI GGWCC 2 cut(s) 268, 284
XmiI GTMKAC 1 cut(s) 354
ZraI GACGTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.