MD05G1201800.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002250
Physical Location & Seq
Forward (+)
32892135 .. 32895178
3044 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1201800.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGACCCGATTTCCACCTCCATCTCCTCCTCCTTCTTCTTCCTCTTCGCTATTGATTCCGACCGACGAATCCCTAACCCTAATTCCCGGCCTCCCAAACGACGTCTCTGCGTTGATCCTCTCCTTCATCCCCTTCTCCCACCAGGCCCGTCTCCGACCCACCTGCAAATCATGGAGGCTCTTCCTCTCCTCCAAAACCCTAATCGCCCTCCGCCAGACCCACCACCGCAGCAACCTCTCCCACCTCCTCTGCGTCTTCCCCCAGGACCCCTCCGTCGCCGCCCCTTTCCTCTTCGATCCCCGAACCCTAGCCTGGTGCCCCCTCCCGCCCATGCCCTGCAACCCCCACGTCTACGGACTCTGCAACTTCACCTCCCTCTCCATTGGGCCCCATCTCTACGTCATCGGCGGCTCGCTCTTCGACACTCGTTCGTTTCCGATCGACCGCCCCTCGCCGTCCTCCGCGGTCTTCCGATTTGACTTCAGCACTTCCTCGTGGAGCTTCCTCGCTCCTATGCTCACGCCGCGCGGAAGTTTCGCCTGCGCCGCGGTTCCAAATTCGGGAGAGATTCTCGTCGCTGGCGGCGGCTCCCGGCACACTCTGTTCAGCGCGGCTGGAAGCCGAATGAGCTCAGTCGAGCGGTATGACATTGGTAGAAATGAATGGGTTGCAATGGACGGGTTGCCAGGTTTTCGGGCTGGGTGCGCGGGGTTCTTTGTTGGGGATGGCGAGGAGAGGGAGTTCTGGGTTATGGGTGGGTACGGCGAGTCCAGAACAATATCAGGGGTTTTTCCGGTGGACGAGCATTACAGAGACGCTGTGGTAATGGAATTGAAGAATGGGAATGGTGGTTGCAGGTGGCGAGAGGTTGGAGAGATGTGGGAAGCAGGGGAGAGGATGAGACTGGGCAAAACTGTTGTCGTGGAAGATGGCGATGATCGCAGTCGACCTGCTGTTTTCATGCTTGATCGGAATGTTATATTCAGATACGACATGGCTTCAAACCGTTGGCGTAAGGAATCACGTCTACCAAGACAGGTCCCATGCGACTCAGAATTTGGATTCGTTGTATTGGATGGAGAGTTGCATGTAATAACCCTTTTGAAGGCAGTTGAACCAGCAGAAATCCGCAGGTCACGACTGCGTAAGAGAGCAGGAACACTTTACATCCAAATCTACCATCCAAAGAAGAAGACATGGAGATCTCTCATAACAAAGTCACCTTTCAATTACGGCTTGGATTTCAATACTGCAGTTATGAGCACCATTCGCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

426

Amino Acids

47.5

Weight (kDa)

9.17

Isoelectric Point (pI)

52.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_1 PF01344 121 - 172 2.7e-07 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 170, 846
AasI GACNNNNNNGTC 1 cut(s) 272
AatII GACGTC 1 cut(s) 105
Acc36I ACCTGC 4 cut(s) 170, 846, 958, 1122
AccB1I GGYRCC 1 cut(s) 315
AccBSI CCGCTC 1 cut(s) 640
AccI GTMKAC 3 cut(s) 351, 946, 1027
AccII CGCG 6 cut(s) 464, 526, 528, 548, 611, 707
AclWI GGATC 2 cut(s) 109, 290
AcsI RAATTY 2 cut(s) 556, 1055
AcuI CTGAAG 1 cut(s) 466
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 1 cut(s) 761
AfiI CCNNNNNNNGG 3 cut(s) 312, 560, 1105
AgsI TTSAA 6 cut(s) 835, 1002, 1105, 1115, 1228, 1246
AjiI CACGTC 2 cut(s) 349, 1025
AjnI CCWGG 4 cut(s) 141, 261, 311, 685
AloI GAACNNNNNNTCC 2 cut(s) 725, 757
AluBI AGCT 2 cut(s) 501, 630
AluI AGCT 2 cut(s) 501, 630
Alw21I GWGCWC 2 cut(s) 632, 1265
Alw26I GTCTC 4 cut(s) 109, 155, 807, 895
AlwI GGATC 2 cut(s) 109, 290
AoxI GGCC 3 cut(s) 88, 144, 386
ApaI GGGCCC 1 cut(s) 390
ApeKI GCWGC 1 cut(s) 228
ApoI RAATTY 2 cut(s) 556, 1055
ArsI GACNNNNNNTTYG 4 cut(s) 903, 935, 1040, 1072
AspLEI GCGC 4 cut(s) 528, 545, 611, 707
AspS9I GGNCC 5 cut(s) 145, 265, 386, 387, 1039
AsuC2I CCSGG 2 cut(s) 87, 592
AsuHPI GGTGA 2 cut(s) 361, 1212
AvaII GGWCC 2 cut(s) 265, 1039
BaeGI GKGCMC 2 cut(s) 320, 390
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 2 cut(s) 390, 632
BauI CACGAG 1 cut(s) 493
BbsI GAAGAC 3 cut(s) 247, 460, 1199
Bbv12I GWGCWC 2 cut(s) 632, 1265
BbvI GCAGC 1 cut(s) 240
BccI CCATC 6 cut(s) 28, 399, 719, 923, 1070, 1188
BceAI ACGGC 3 cut(s) 439, 778, 1249
BcgI CGANNNNNNTGC 4 cut(s) 89, 123, 144, 178
BciT130I CCWGG 4 cut(s) 143, 263, 313, 687
BcnI CCSGG 2 cut(s) 87, 592
BcoDI GTCTC 4 cut(s) 109, 155, 807, 895
BfaI CTAG 1 cut(s) 308
BfmI CTRYAG 1 cut(s) 1251
BfuAI ACCTGC 4 cut(s) 170, 846, 958, 1122
BglII AGATCT 1 cut(s) 1202
BisI GCNGC 8 cut(s) 229, 279, 409, 524, 546, 583, 586, 612
BlsI GCNGC 8 cut(s) 230, 280, 410, 525, 547, 584, 587, 613
Bme1390I CCNGG 6 cut(s) 87, 143, 263, 313, 592, 687
Bme18I GGWCC 2 cut(s) 265, 1039
BmgBI CACGTC 2 cut(s) 349, 1025
BmgT120I GGNCC 5 cut(s) 145, 265, 386, 387, 1039
BmiI GGNNCC 7 cut(s) 267, 317, 388, 389, 552, 589, 1041
BmrFI CCNGG 6 cut(s) 87, 143, 263, 313, 592, 687
BmrI ACTGGG 1 cut(s) 914
BmuI ACTGGG 1 cut(s) 914
BpiI GAAGAC 3 cut(s) 247, 460, 1199
BplI GAGNNNNNCTC 2 cut(s) 555, 587
BpuMI CCSGG 2 cut(s) 87, 592
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 3 cut(s) 261, 462, 546
BsaWI WCCGGW 1 cut(s) 793
BsaXI ACNNNNNCTCC 4 cut(s) 356, 386, 725, 755
Bsc4I CCNNNNNNNGG 3 cut(s) 312, 560, 1105
Bse1I ACTGG 1 cut(s) 909
Bse3DI GCAATG 1 cut(s) 678
BseBI CCWGG 4 cut(s) 143, 263, 313, 687
BseDI CCNNGG 3 cut(s) 261, 462, 546
BseGI GGATG 6 cut(s) 126, 730, 903, 1081, 1167, 1180
BseLI CCNNNNNNNGG 3 cut(s) 312, 560, 1105
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 2 cut(s) 645, 1065
BseNI ACTGG 1 cut(s) 909
BseRI GAGGAG 5 cut(s) 15, 18, 178, 236, 746
BseSI GKGCMC 2 cut(s) 320, 390
BseXI GCAGC 1 cut(s) 240
BseYI CCCAGC 1 cut(s) 698
Bsh1236I CGCG 6 cut(s) 464, 526, 528, 548, 611, 707
Bsh1285I CGRYCG 3 cut(s) 63, 441, 445
BshFI GGCC 3 cut(s) 90, 146, 388
BshNI GGYRCC 1 cut(s) 315
BsiEI CGRYCG 3 cut(s) 63, 441, 445
BsiHKAI GWGCWC 2 cut(s) 632, 1265
BsiSI CCGG 3 cut(s) 87, 592, 794
BslFI GGGAC 1 cut(s) 1025
BslI CCNNNNNNNGG 3 cut(s) 312, 560, 1105
BsmAI GTCTC 4 cut(s) 109, 155, 807, 895
BsmBI CGTCTC 3 cut(s) 109, 155, 807
BsmFI GGGAC 1 cut(s) 1025
BsnI GGCC 3 cut(s) 90, 146, 388
Bsp120I GGGCCC 1 cut(s) 386
Bsp1286I GDGCHC 4 cut(s) 320, 390, 632, 1265
Bsp143I GATC 6 cut(s) 114, 295, 438, 937, 967, 1202
BspANI GGCC 3 cut(s) 90, 146, 388
BspCNI CTCAG 2 cut(s) 644, 1064
BspFNI CGCG 6 cut(s) 464, 526, 528, 548, 611, 707
BspLI GGNNCC 7 cut(s) 267, 317, 388, 389, 552, 589, 1041
BspMAI CTGCAG 1 cut(s) 1255
BspMI ACCTGC 4 cut(s) 170, 846, 958, 1122
BspPI GGATC 2 cut(s) 109, 290
BspQI GCTCTTC 2 cut(s) 185, 422
BspT107I GGYRCC 1 cut(s) 315
BsrBI CCGCTC 1 cut(s) 640
BsrDI GCAATG 1 cut(s) 678
BsrI ACTGG 1 cut(s) 909
BssECI CCNNGG 3 cut(s) 261, 462, 546
BssMI GATC 6 cut(s) 114, 295, 438, 937, 967, 1202
BssNI GRCGYC 1 cut(s) 102
BssSI CACGAG 1 cut(s) 493
Bst2BI CACGAG 1 cut(s) 493
Bst2UI CCWGG 4 cut(s) 143, 263, 313, 687
Bst4CI ACNGT 2 cut(s) 916, 1007
Bst6I CTCTTC 4 cut(s) 49, 185, 296, 422
BstACI GRCGYC 1 cut(s) 102
BstC8I GCNNGC 3 cut(s) 413, 541, 580
BstDEI CTNAG 2 cut(s) 631, 1051
BstDSI CCRYGG 2 cut(s) 462, 546
BstENI CCTNNNNNAGG 1 cut(s) 1103
BstF5I GGATG 6 cut(s) 126, 730, 903, 1081, 1167, 1180
BstFNI CGCG 6 cut(s) 464, 526, 528, 548, 611, 707
BstHHI GCGC 4 cut(s) 528, 545, 611, 707
BstKTI GATC 6 cut(s) 117, 298, 441, 940, 970, 1205
BstMAI GTCTC 4 cut(s) 109, 155, 807, 895
BstMBI GATC 6 cut(s) 114, 295, 438, 937, 967, 1202
BstMCI CGRYCG 3 cut(s) 63, 441, 445
BstMWI GCNNNNNNNGC 6 cut(s) 523, 545, 627, 704, 939, 1269
BstNI CCWGG 4 cut(s) 143, 263, 313, 687
BstNSI RCATGY 1 cut(s) 1091
BstSCI CCNGG 6 cut(s) 85, 141, 261, 311, 590, 685
BstSFI CTRYAG 1 cut(s) 1251
BstSLI GKGCMC 2 cut(s) 320, 390
BstUI CGCG 6 cut(s) 464, 526, 528, 548, 611, 707
BstV1I GCAGC 1 cut(s) 240
BstV2I GAAGAC 3 cut(s) 247, 460, 1199
BstX2I RGATCY 1 cut(s) 1202
BstYI RGATCY 1 cut(s) 1202
BsuRI GGCC 3 cut(s) 90, 146, 388
BtgI CCRYGG 2 cut(s) 462, 546
BtgZI GCGATG 1 cut(s) 948
BtrI CACGTC 2 cut(s) 349, 1025
BtsCI GGATG 6 cut(s) 126, 730, 903, 1081, 1167, 1180
BveI ACCTGC 4 cut(s) 170, 846, 958, 1122
Cac8I GCNNGC 3 cut(s) 413, 541, 580
CfoI GCGC 4 cut(s) 528, 545, 611, 707
Cfr13I GGNCC 5 cut(s) 145, 265, 386, 387, 1039
Cfr42I CCGCGG 2 cut(s) 465, 549
CseI GACGC 2 cut(s) 241, 824
Csp6I GTAC 1 cut(s) 760
CviAII CATG 7 cut(s) 171, 331, 961, 994, 1044, 1088, 1197
CviQI GTAC 1 cut(s) 760
DdeI CTNAG 2 cut(s) 631, 1051
DpnI GATC 6 cut(s) 116, 297, 440, 939, 969, 1204
DpnII GATC 6 cut(s) 114, 295, 438, 937, 967, 1202
DrdI GACNNNNNNGTC 1 cut(s) 272
DseDI GACNNNNNNGTC 1 cut(s) 272
Eam1104I CTCTTC 4 cut(s) 49, 185, 296, 422
EarI CTCTTC 4 cut(s) 49, 185, 296, 422
EciI GGCGGA 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 630
Eco24I GRGCYC 2 cut(s) 390, 632
Eco47I GGWCC 2 cut(s) 265, 1039
Eco53kI GAGCTC 1 cut(s) 630
Eco57I CTGAAG 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 630
EcoNI CCTNNNNNAGG 1 cut(s) 1103
EcoO109I RGGNCCY 3 cut(s) 265, 387, 1039
EcoRII CCWGG 4 cut(s) 141, 261, 311, 685
EcoT38I GRGCYC 2 cut(s) 390, 632
Esp3I CGTCTC 3 cut(s) 109, 155, 807
FaeI CATG 7 cut(s) 174, 334, 964, 997, 1047, 1091, 1200
FaqI GGGAC 1 cut(s) 1025
FatI CATG 7 cut(s) 170, 330, 960, 993, 1043, 1087, 1196
FauI CCCGC 2 cut(s) 333, 700
FblI GTMKAC 3 cut(s) 351, 946, 1027
Fnu4HI GCNGC 8 cut(s) 229, 279, 409, 524, 546, 583, 586, 612
FokI GGATG 6 cut(s) 113, 737, 910, 1088, 1154, 1167
FriOI GRGCYC 2 cut(s) 390, 632
Fsp4HI GCNGC 8 cut(s) 229, 279, 409, 524, 546, 583, 586, 612
FspBI CTAG 1 cut(s) 308
GlaI GCGC 4 cut(s) 527, 544, 610, 706
GluI GCNGC 8 cut(s) 229, 279, 409, 524, 546, 583, 586, 612
GsaI CCCAGC 1 cut(s) 702
HaeIII GGCC 3 cut(s) 90, 146, 388
HapII CCGG 3 cut(s) 87, 592, 794
HgaI GACGC 2 cut(s) 241, 824
HhaI GCGC 4 cut(s) 528, 545, 611, 707
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 7 cut(s) 174, 334, 964, 997, 1047, 1091, 1200
Hin6I GCGC 4 cut(s) 526, 543, 609, 705
HinP1I GCGC 4 cut(s) 526, 543, 609, 705
HincII GTYRAC 1 cut(s) 947
HindII GTYRAC 1 cut(s) 947
HinfI GANTC 8 cut(s) 55, 68, 357, 568, 767, 1019, 1049, 1062
HpaII CCGG 3 cut(s) 87, 592, 794
HphI GGTGA 2 cut(s) 361, 1212
Hpy166II GTNNAC 4 cut(s) 352, 799, 947, 1028
Hpy188I TCNGA 7 cut(s) 60, 155, 438, 473, 972, 986, 1054
Hpy188III TCNNGA 3 cut(s) 561, 771, 1137
Hpy8I GTNNAC 4 cut(s) 352, 799, 947, 1028
Hpy99I CGWCG 4 cut(s) 68, 104, 278, 578
HpyAV CCTTC 4 cut(s) 42, 133, 142, 1099
HpyCH4III ACNGT 2 cut(s) 916, 1007
HpyCH4IV ACGT 4 cut(s) 102, 348, 399, 1024
HpyCH4V TGCA 7 cut(s) 165, 339, 363, 671, 855, 1087, 1253
HpyF10VI GCNNNNNNNGC 6 cut(s) 523, 545, 627, 704, 939, 1269
HpyF3I CTNAG 2 cut(s) 631, 1051
HpySE526I ACGT 4 cut(s) 102, 348, 399, 1024
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 7 cut(s) 174, 334, 964, 997, 1047, 1091, 1200
HspAI GCGC 4 cut(s) 526, 543, 609, 705
KspI CCGCGG 2 cut(s) 465, 549
Kzo9I GATC 6 cut(s) 114, 295, 438, 937, 967, 1202
LguI GCTCTTC 2 cut(s) 185, 422
LmnI GCTCC 3 cut(s) 498, 514, 593
Lsp1109I GCAGC 1 cut(s) 240
MaeI CTAG 1 cut(s) 308
MaeII ACGT 4 cut(s) 102, 348, 399, 1024
MaeIII GTNAC 2 cut(s) 1134, 1218
MalI GATC 6 cut(s) 116, 297, 440, 939, 969, 1204
MbiI CCGCTC 1 cut(s) 640
MboI GATC 6 cut(s) 114, 295, 438, 937, 967, 1202
MflI RGATCY 1 cut(s) 1202
MhlI GDGCHC 4 cut(s) 320, 390, 632, 1265
MluCI AATT 5 cut(s) 81, 556, 830, 1055, 1228
MlyI GAGTC 3 cut(s) 351, 776, 1043
MmeI TCCRAC 3 cut(s) 83, 178, 850
MspA1I CMGCKG 2 cut(s) 464, 548
MspI CCGG 3 cut(s) 87, 592, 794
MspR9I CCNGG 6 cut(s) 87, 143, 263, 313, 592, 687
MvaI CCWGG 4 cut(s) 143, 263, 313, 687
MvnI CGCG 6 cut(s) 464, 526, 528, 548, 611, 707
MwoI GCNNNNNNNGC 6 cut(s) 523, 545, 627, 704, 939, 1269
NciI CCSGG 2 cut(s) 87, 592
NdeII GATC 6 cut(s) 114, 295, 438, 937, 967, 1202
NlaIII CATG 7 cut(s) 174, 334, 964, 997, 1047, 1091, 1200
NlaIV GGNNCC 7 cut(s) 267, 317, 388, 389, 552, 589, 1041
NmuCI GTSAC 2 cut(s) 1134, 1218
NspI RCATGY 1 cut(s) 1091
PaqCI CACCTGC 2 cut(s) 170, 846
PciSI GCTCTTC 2 cut(s) 185, 422
PfeI GAWTC 5 cut(s) 55, 68, 568, 1019, 1062
PkrI GCNGC 8 cut(s) 230, 280, 410, 525, 547, 584, 587, 613
Ple19I CGATCG 1 cut(s) 441
PleI GAGTC 3 cut(s) 351, 775, 1043
PpsI GAGTC 3 cut(s) 351, 775, 1043
PpuMI RGGWCCY 2 cut(s) 265, 1039
Psp124BI GAGCTC 1 cut(s) 632
Psp5II RGGWCCY 2 cut(s) 265, 1039
Psp6I CCWGG 4 cut(s) 141, 261, 311, 685
PspFI CCCAGC 1 cut(s) 698
PspGI CCWGG 4 cut(s) 141, 261, 311, 685
PspN4I GGNNCC 7 cut(s) 267, 317, 388, 389, 552, 589, 1041
PspOMI GGGCCC 1 cut(s) 386
PspPI GGNCC 5 cut(s) 145, 265, 386, 387, 1039
PspPPI RGGWCCY 2 cut(s) 265, 1039
PstI CTGCAG 1 cut(s) 1255
PsuI RGATCY 1 cut(s) 1202
PvuI CGATCG 1 cut(s) 441
RsaI GTAC 1 cut(s) 761
RsaNI GTAC 1 cut(s) 760
SacI GAGCTC 1 cut(s) 632
SacII CCGCGG 2 cut(s) 465, 549
SalI GTCGAC 1 cut(s) 945
SapI GCTCTTC 2 cut(s) 185, 422
SatI GCNGC 8 cut(s) 229, 279, 409, 524, 546, 583, 586, 612
Sau3AI GATC 6 cut(s) 114, 295, 438, 937, 967, 1202
Sau96I GGNCC 5 cut(s) 145, 265, 386, 387, 1039
SchI GAGTC 3 cut(s) 351, 776, 1043
ScrFI CCNGG 6 cut(s) 87, 143, 263, 313, 592, 687
SduI GDGCHC 4 cut(s) 320, 390, 632, 1265
SfcI CTRYAG 1 cut(s) 1251
Sfr303I CCGCGG 2 cut(s) 465, 549
SgrBI CCGCGG 2 cut(s) 465, 549
SinI GGWCC 2 cut(s) 265, 1039
Sse9I AATT 5 cut(s) 81, 556, 830, 1055, 1228
SspMI CTAG 1 cut(s) 308
SstI GAGCTC 1 cut(s) 632
StyD4I CCNGG 6 cut(s) 85, 141, 261, 311, 590, 685
TaaI ACNGT 2 cut(s) 916, 1007
TaiI ACGT 4 cut(s) 105, 351, 402, 1027
TaqI TCGA 5 cut(s) 294, 420, 441, 636, 946
TaqII GACCGA 1 cut(s) 77
TasI AATT 5 cut(s) 81, 556, 830, 1055, 1228
TauI GCSGC 7 cut(s) 281, 411, 526, 548, 585, 588, 614
TfiI GAWTC 5 cut(s) 55, 68, 568, 1019, 1062
TseFI GTSAC 2 cut(s) 1134, 1218
TseI GCWGC 1 cut(s) 228
Tsp45I GTSAC 2 cut(s) 1134, 1218
TspDTI ATGAA 3 cut(s) 115, 675, 949
TspGWI ACGGA 2 cut(s) 262, 369
VpaK11BI GGWCC 2 cut(s) 265, 1039
XagI CCTNNNNNAGG 1 cut(s) 1103
XapI RAATTY 2 cut(s) 556, 1055
XceI RCATGY 1 cut(s) 1091
XmiI GTMKAC 3 cut(s) 351, 946, 1027
XspI CTAG 1 cut(s) 308
ZraI GACGTC 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.