AT5G04220

Synaptotagmin-like mitochondrial-lipid-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001241
Physical Location & Seq
Reverse (-)
1155725 .. 1158944
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04220.3

Sequence Viewer

Length: 957 bp
ATGATCCTCCTATCGGAAACTATTAAAAGGCAAGTTTCAAGCATGTACCATTGGCCACAAGTGCTTGAGATACCTATTCTTGATTCTTCAACAGCGTCTGTAAAGAAGCCTGTTGGATTACTACATGTGAGCATTCTTCGAGCTCGTAATCTCCTCAAGAAGGATTTGTTGGGAACATCCGACCCTTATGTCAAGCTTAGCTTAACTGGAGAAAAGCTTCCAGCAAAGAAAACCACCATAAAGAAGAGGAACTTAAACCCGGAATGGAACGAACACTTTAAGCTCATCGTCAAGGACCCAAACTCACAAGTCCTTCAACTAGAAGTCTTTGACTGGGACAAGGTCGGTGGGCACGACAGATTAGGAATGCAGATGATCCCTTTGCAAAAGATTAACCCGGGTGAGAGGAAGGAGTTTAATCTTGATCTGATTAAGAACTCAAATGTTGTTATGGATTCTGGAGACAAGAAAAAAAGAGGAAGATTAGAGGTTGATCTCAGGTATGTGCCATTCAGAGAAGAAAGCATTAAGCGTAGAAAAGAGAGCAGAGAAGAGAAATCATCAGAGGACGATGATTTTCTCAGCCAAGCCGGTCTACTCTCGGTTGCAGTCCAATCTGCAAAAGATGTCGAGGGCAAGAAGAAACACTCGAACCCTTATGCTGTTGTTCTCTTCAGAGGAGAAAAGAAGAAAACTAAGATGCTGAAGAAAACACGAGACCCTCGATGGAACGAGGAATTCCAGTTTACACTAGAGGAGCCTCCAGTGAAAGAGAGTATACGCGTTGAGGTCATGAGTAAAGGAACCGGCTTTCACTTTCGATCCAAGGAGGAACTAGGACATGTAGACATAAATCTGGACGATGTAGTTGACAATGGAAGGATCAACCAGAAATACCATCTTATCAATTCCAGAAACGGCATTATTCACATTGAGATCCGTTGGACAACTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 SMP_LBD

Protein Analysis

318

Amino Acids

36.76

Weight (kDa)

9.55

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMP_LBD PF17047 5 - 185 7.6e-16 Synaptotagmin-like mitochondrial-lipid-binding domain
C2 PF00168 199 - 301 5.9e-28 C2 domain
C2 PF00168 358 - 461 4.4e-19 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AccI GTMKAC 3 cut(s) 595, 778, 846
AccII CGCG 1 cut(s) 783
AclWI GGATC 4 cut(s) 370, 816, 890, 931
AcoI YGGCCR 1 cut(s) 53
AcsI RAATTY 1 cut(s) 737
AcuI CTGAAG 2 cut(s) 658, 725
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 2 cut(s) 13, 160
AflIII ACRYGT 3 cut(s) 124, 781, 841
AgsI TTSAA 3 cut(s) 39, 90, 317
AhlI ACTAGT 1 cut(s) 950
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 5 cut(s) 143, 196, 201, 217, 283
AluI AGCT 5 cut(s) 143, 196, 201, 217, 283
Alw21I GWGCWC 1 cut(s) 145
Alw26I GTCTC 2 cut(s) 456, 711
AlwI GGATC 4 cut(s) 370, 816, 890, 931
AlwNI CAGNNNCTG 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 397
AoxI GGCC 1 cut(s) 53
ApoI RAATTY 1 cut(s) 737
Asp700I GAANNNNTTC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 295
AsuC2I CCSGG 3 cut(s) 260, 398, 399
AsuHPI GGTGA 1 cut(s) 413
AvaI CYCGRG 1 cut(s) 397
AvaII GGWCC 1 cut(s) 295
BaeGI GKGCMC 1 cut(s) 354
BalI TGGCCA 1 cut(s) 55
BanII GRGCYC 1 cut(s) 145
BauI CACGAG 1 cut(s) 714
Bbv12I GWGCWC 1 cut(s) 145
BccI CCATC 2 cut(s) 720, 906
BceAI ACGGC 1 cut(s) 934
BcnI CCSGG 3 cut(s) 260, 398, 399
BcoDI GTCTC 2 cut(s) 456, 711
BcuI ACTAGT 1 cut(s) 950
BfaI CTAG 4 cut(s) 320, 752, 836, 951
BlpI GCTNAGC 1 cut(s) 197
Bme1390I CCNGG 3 cut(s) 260, 398, 399
Bme18I GGWCC 1 cut(s) 295
BmeT110I CYCGRG 1 cut(s) 397
BmgT120I GGNCC 1 cut(s) 295
BmiI GGNNCC 3 cut(s) 297, 759, 805
BmrFI CCNGG 3 cut(s) 260, 398, 399
BmrI ACTGGG 1 cut(s) 343
BmsI GCATC 1 cut(s) 690
BmuI ACTGGG 1 cut(s) 343
BpmI CTGGAG 3 cut(s) 228, 480, 747
Bpu1102I GCTNAGC 1 cut(s) 197
BpuEI CTTGAG 2 cut(s) 86, 140
BpuMI CCSGG 3 cut(s) 260, 398, 399
BsaI GGTCTC 1 cut(s) 711
BsaJI CCNNGG 2 cut(s) 397, 825
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 160
Bse118I RCCGGY 2 cut(s) 590, 806
Bse1I ACTGG 4 cut(s) 211, 338, 742, 764
BseDI CCNNGG 2 cut(s) 397, 825
BseGI GGATG 1 cut(s) 176
BseLI CCNNNNNNNGG 2 cut(s) 13, 160
BseMII CTCAG 2 cut(s) 511, 595
BseNI ACTGG 4 cut(s) 211, 338, 742, 764
BseRI GAGGAG 3 cut(s) 143, 693, 770
BseSI GKGCMC 1 cut(s) 354
Bsh1236I CGCG 1 cut(s) 783
BshFI GGCC 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 145
BsiHKCI CYCGRG 1 cut(s) 397
BsiSI CCGG 4 cut(s) 260, 398, 591, 807
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 13, 160
BsmAI GTCTC 2 cut(s) 456, 711
BsmFI GGGAC 1 cut(s) 350
BsmI GAATGC 2 cut(s) 132, 372
BsnI GGCC 1 cut(s) 55
Bso31I GGTCTC 1 cut(s) 711
BsoBI CYCGRG 1 cut(s) 397
Bsp1286I GDGCHC 2 cut(s) 145, 354
Bsp143I GATC 7 cut(s) 3, 375, 424, 493, 821, 882, 936
Bsp1720I GCTNAGC 1 cut(s) 197
BspANI GGCC 1 cut(s) 55
BspCNI CTCAG 2 cut(s) 510, 594
BspFNI CGCG 1 cut(s) 783
BspHI TCATGA 1 cut(s) 792
BspLI GGNNCC 3 cut(s) 297, 759, 805
BspPI GGATC 4 cut(s) 370, 816, 890, 931
BspTNI GGTCTC 1 cut(s) 711
BsrFI RCCGGY 2 cut(s) 590, 806
BsrI ACTGG 4 cut(s) 211, 338, 742, 764
BssAI RCCGGY 2 cut(s) 590, 806
BssECI CCNNGG 2 cut(s) 397, 825
BssMI GATC 7 cut(s) 3, 375, 424, 493, 821, 882, 936
BssNAI GTATAC 1 cut(s) 779
BssSI CACGAG 1 cut(s) 714
BssT1I CCWWGG 1 cut(s) 825
Bst1107I GTATAC 1 cut(s) 779
Bst2BI CACGAG 1 cut(s) 714
Bst6I CTCTTC 3 cut(s) 239, 546, 677
BstDEI CTNAG 4 cut(s) 197, 497, 581, 696
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 1 cut(s) 176
BstFNI CGCG 1 cut(s) 783
BstKTI GATC 7 cut(s) 6, 378, 427, 496, 824, 885, 939
BstMAI GTCTC 2 cut(s) 456, 711
BstMBI GATC 7 cut(s) 3, 375, 424, 493, 821, 882, 936
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstNSI RCATGY 3 cut(s) 46, 128, 845
BstSCI CCNGG 3 cut(s) 258, 396, 397
BstSLI GKGCMC 1 cut(s) 354
BstUI CGCG 1 cut(s) 783
BstX2I RGATCY 1 cut(s) 936
BstYI RGATCY 1 cut(s) 936
BstZ17I GTATAC 1 cut(s) 779
BsuRI GGCC 1 cut(s) 55
BtsCI GGATG 1 cut(s) 176
BtsIMutI CAGTG 1 cut(s) 771
CaiI CAGNNNCTG 1 cut(s) 98
CciI TCATGA 1 cut(s) 792
Cfr10I RCCGGY 2 cut(s) 590, 806
Cfr13I GGNCC 1 cut(s) 295
Cfr9I CCCGGG 1 cut(s) 397
CseI GACGC 1 cut(s) 84
Csp6I GTAC 1 cut(s) 46
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 4 cut(s) 43, 125, 793, 842
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 4 cut(s) 197, 497, 581, 696
DpnI GATC 7 cut(s) 5, 377, 426, 495, 823, 884, 938
DpnII GATC 7 cut(s) 3, 375, 424, 493, 821, 882, 936
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 53
Eam1104I CTCTTC 3 cut(s) 239, 546, 677
EarI CTCTTC 3 cut(s) 239, 546, 677
Ecl136II GAGCTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 825
Eco24I GRGCYC 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 711
Eco47I GGWCC 1 cut(s) 295
Eco53kI GAGCTC 1 cut(s) 143
Eco57I CTGAAG 2 cut(s) 658, 725
Eco88I CYCGRG 1 cut(s) 397
EcoICRI GAGCTC 1 cut(s) 143
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 295
EcoRI GAATTC 1 cut(s) 737
EcoT14I CCWWGG 1 cut(s) 825
EcoT38I GRGCYC 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 825
FaeI CATG 4 cut(s) 46, 128, 796, 845
FalI AAGNNNNNCTT 4 cut(s) 185, 217, 236, 268
FaqI GGGAC 1 cut(s) 350
FatI CATG 4 cut(s) 42, 124, 792, 841
FblI GTMKAC 3 cut(s) 595, 778, 846
FokI GGATG 1 cut(s) 163
FriOI GRGCYC 1 cut(s) 145
FspBI CTAG 4 cut(s) 320, 752, 836, 951
GsuI CTGGAG 3 cut(s) 228, 480, 747
HaeIII GGCC 1 cut(s) 55
HapII CCGG 4 cut(s) 260, 398, 591, 807
HgaI GACGC 1 cut(s) 84
Hin1II CATG 4 cut(s) 46, 128, 796, 845
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HindIII AAGCTT 2 cut(s) 194, 215
HinfI GANTC 2 cut(s) 83, 455
HpaII CCGG 4 cut(s) 260, 398, 591, 807
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 5 cut(s) 596, 747, 779, 847, 871
Hpy188I TCNGA 6 cut(s) 16, 181, 429, 515, 565, 677
Hpy188III TCNNGA 7 cut(s) 80, 157, 422, 459, 793, 857, 912
Hpy8I GTNNAC 5 cut(s) 596, 747, 779, 847, 871
HpyAV CCTTC 4 cut(s) 154, 323, 403, 873
HpyCH4V TGCA 4 cut(s) 370, 385, 608, 620
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
HpyF3I CTNAG 4 cut(s) 197, 497, 581, 696
Hsp92II CATG 4 cut(s) 46, 128, 796, 845
Kzo9I GATC 7 cut(s) 3, 375, 424, 493, 821, 882, 936
LmnI GCTCC 1 cut(s) 757
LweI GCATC 1 cut(s) 690
MaeI CTAG 4 cut(s) 320, 752, 836, 951
MalI GATC 7 cut(s) 5, 377, 426, 495, 823, 884, 938
MboI GATC 7 cut(s) 3, 375, 424, 493, 821, 882, 936
MflI RGATCY 1 cut(s) 936
MhlI GDGCHC 2 cut(s) 145, 354
MlsI TGGCCA 1 cut(s) 55
MluCI AATT 2 cut(s) 737, 907
MluI ACGCGT 1 cut(s) 781
MluNI TGGCCA 1 cut(s) 55
MmeI TCCRAC 3 cut(s) 94, 204, 923
Mox20I TGGCCA 1 cut(s) 55
MroXI GAANNNNTTC 1 cut(s) 216
MscI TGGCCA 1 cut(s) 55
MseI TTAA 9 cut(s) 24, 203, 254, 279, 393, 417, 432, 528, 955
Msp20I TGGCCA 1 cut(s) 55
MspI CCGG 4 cut(s) 260, 398, 591, 807
MspR9I CCNGG 3 cut(s) 260, 398, 399
Mva1269I GAATGC 2 cut(s) 132, 372
MvnI CGCG 1 cut(s) 783
MwoI GCNNNNNNNGC 1 cut(s) 61
NciI CCSGG 3 cut(s) 260, 398, 399
NdeII GATC 7 cut(s) 3, 375, 424, 493, 821, 882, 936
NlaIII CATG 4 cut(s) 46, 128, 796, 845
NlaIV GGNNCC 3 cut(s) 297, 759, 805
NspI RCATGY 3 cut(s) 46, 128, 845
PagI TCATGA 1 cut(s) 792
PciI ACATGT 2 cut(s) 124, 841
PcsI WCGNNNNNNNCGW 2 cut(s) 351, 721
PctI GAATGC 2 cut(s) 132, 372
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 83, 455
PflFI GACNNNGTC 1 cut(s) 341
PpuMI RGGWCCY 1 cut(s) 295
PscI ACATGT 2 cut(s) 124, 841
Psp124BI GAGCTC 1 cut(s) 145
Psp5II RGGWCCY 1 cut(s) 295
PspN4I GGNNCC 3 cut(s) 297, 759, 805
PspPI GGNCC 1 cut(s) 295
PspPPI RGGWCCY 1 cut(s) 295
PstNI CAGNNNCTG 1 cut(s) 98
PsuI RGATCY 1 cut(s) 936
PsyI GACNNNGTC 1 cut(s) 341
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
SacI GAGCTC 1 cut(s) 145
SaqAI TTAA 9 cut(s) 24, 203, 254, 279, 393, 417, 432, 528, 955
Sau3AI GATC 7 cut(s) 3, 375, 424, 493, 821, 882, 936
Sau96I GGNCC 1 cut(s) 295
ScrFI CCNGG 3 cut(s) 260, 398, 399
SduI GDGCHC 2 cut(s) 145, 354
SfaNI GCATC 1 cut(s) 690
SinI GGWCC 1 cut(s) 295
SmaI CCCGGG 1 cut(s) 399
SmlI CTYRAG 2 cut(s) 65, 155
SmoI CTYRAG 2 cut(s) 65, 155
SpeI ACTAGT 1 cut(s) 950
Sse9I AATT 2 cut(s) 737, 907
SspMI CTAG 4 cut(s) 320, 752, 836, 951
SstI GAGCTC 1 cut(s) 145
StyD4I CCNGG 3 cut(s) 258, 396, 397
StyI CCWWGG 1 cut(s) 825
TaqI TCGA 5 cut(s) 139, 630, 650, 724, 820
TasI AATT 2 cut(s) 737, 907
TfiI GAWTC 2 cut(s) 83, 455
Tru1I TTAA 9 cut(s) 24, 203, 254, 279, 393, 417, 432, 528, 955
Tru9I TTAA 9 cut(s) 24, 203, 254, 279, 393, 417, 432, 528, 955
TscAI CASTG 1 cut(s) 771
TspGWI ACGGA 1 cut(s) 929
TspMI CCCGGG 1 cut(s) 397
TspRI CASTG 1 cut(s) 771
Tth111I GACNNNGTC 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 295
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 1 cut(s) 737
XceI RCATGY 3 cut(s) 46, 128, 845
XmaI CCCGGG 1 cut(s) 397
XmiI GTMKAC 3 cut(s) 595, 778, 846
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 4 cut(s) 320, 752, 836, 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.