FvH4_6g16032

Synaptotagmin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001241
Physical Location & Seq
Reverse (-)
10039996 .. 10043992
3997 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16032.t1

Sequence Viewer

Length: 1728 bp
ATGAGACATCTGTATTTCATATTCGAGTTTACTGGATTTCTTATAGGATTTCCAATCGGTCTTCTACTGGGTTTCTTCATATTCATATTCTCATCAAAGCATGTAGATGTGAAGGATCCAGATATAAAATCACTCAGTCAGTGTGATTCAAACTCTCTAGTTGATCTTTTCTCAGAGATTCCTCCGTGGGTGAAGCATCCTGATTTTGAAAGAATTGACTGGTTGAACAAGGCTTTACATGATATGTGGCCTTACCTTGATAAGGCAATATGCAAGATTATCAGAAGCACAGCAGAGCCAATTTTTGCAGAGTACACTGGATGTTTGCAGAGCCAATACTTGCCTACTGAATTCTCAAATATAGGATTTCAGAGCTTGAATCTTGGAACTCTTCCTCCTACAATTTATGGTATCAGAGTGCACGAAACCAACGAAAATGAACTTGTAATTGAACCTGCTATTAGATGGGCAGGAATTCCACACATAACTGTTGTGGTAAAAATACTGTCAGTTCGATTCATCGTTCAGCTAATGGATGTCCAAGTATTTGCAACGCCAAGGATAATCTTAAGGCCTCTCGTACCAACATTCCCATGTTTTGGAAACATAACAGTGACTTTGATAGACAAGCCATATGTGGACTTTGGACTCAGATTACTGGGAGGGGATATTATGGCAATCCCCGGATTCTATCAATTTGTTCAGGAATATATAAGAAATCAAGTTGCTAGCCTTTATTTATGGCCCCAAACTTTGAACATACCCATTCTTCAGGCTTCACTATCAGGAACTATAAAGAAGCCTGTAGGCATACTGCATGTCAAGATTTTAAAAGCTAAGAAACTCTTGAAGATGGATATCATAGGAACATCTGATCCTTATGTTATAATCCAACTAACTGGGGAGAGTCTTCCACCAAAGAAAACTAGCATTAAGATGAGAAACTTGAACCCTGAATGGAATGAGGACTTCAACCTTACAGTCAAAGATCCAGAGTCCCAATTCATTCAGTTTAATGTTGTCGACTGGGATCAGTTTGGGACGCATGACCCTATGGGAATGCAAATTCTTCCACTGAAGTCGCTAACCCCAGATAACACACAAGAATTTACGCTTGATTTGGTGAAGAACACAAACCCTTATGATCCTCAAAACAAGAAAAAAAGAGGCCAACTTGTGGTGCAGTTGACATATAATCCTTTTAAAGAAGAAAGTAGTGGTGGAATACATGACAGTGGACCTTTGGATGGACATCAACTGATAGATGCGAAGAAGCTTGGAATCAAGAGCCGGAATAGGAGCCGAAGGCAATCCATGTCCATAGACATGTCTTCACAATTACATGCAGCTGGTCTGCTTTTGGTTACAGTTCAAGGAGCAGAAGAACTCGAGAGCAAGCTTTATGAGAGCCCTAATCCTTACGTATTGGTTCTGTTTAAAGGAGAAAAGAGGAAAACCAAGATGATAAAGAGATCCCATCGGCCATATTGGAACGAAGAATTTCAGTTTGTGGTAGAAGAGGCGACTTTGAAGGAGAGTATTCGTTTTGCAGTAAAGAGTAAGAAAAGACACCTTTTCGCCTATCGTTTCCGAAAAAAGGAATTGTTGGGGTATATTGACATCAATTTTAGTGATGTGGTATACAATGGGCGCATCAGAGGCGAGTACAATCTGATTAACTCAAGAAATGGAGTTATACATGTCGAGTTGCACTGGAGCACTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 SMP_LBD

Protein Analysis

576

Amino Acids

66.02

Weight (kDa)

8.89

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMP_LBD PF17047 68 - 256 2.8e-16 Synaptotagmin-like mitochondrial-lipid-binding domain
C2 PF00168 270 - 370 3e-24 C2 domain
C2 PF00168 452 - 558 8.8e-16 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 887
Acc36I ACCTGC 1 cut(s) 463
AccB7I CCANNNNNTGG 2 cut(s) 599, 1177
AccI GTMKAC 2 cut(s) 1023, 1641
AclWI GGATC 7 cut(s) 110, 123, 869, 983, 1038, 1139, 1467
AcoI YGGCCR 1 cut(s) 1481
AcsI RAATTY 5 cut(s) 350, 474, 1065, 1106, 1499
AcuI CTGAAG 2 cut(s) 755, 1097
AfaI GTAC 3 cut(s) 314, 582, 1667
AfiI CCNNNNNNNGG 5 cut(s) 262, 599, 1177, 1247, 1597
AflII CTTAAG 1 cut(s) 568
AflIII ACRYGT 2 cut(s) 1326, 1699
AloI GAACNNNNNNTCC 4 cut(s) 379, 411, 859, 891
AluBI AGCT 6 cut(s) 375, 529, 836, 1276, 1349, 1399
AluI AGCT 6 cut(s) 375, 529, 836, 1276, 1349, 1399
Alw21I GWGCWC 2 cut(s) 423, 1721
Alw44I GTGCAC 1 cut(s) 419
AlwI GGATC 7 cut(s) 110, 123, 869, 983, 1038, 1139, 1467
Ama87I CYCGRG 1 cut(s) 1388
AoxI GGCC 5 cut(s) 248, 572, 743, 1168, 1481
ApaLI GTGCAC 1 cut(s) 419
ApeKI GCWGC 1 cut(s) 1346
ApoI RAATTY 5 cut(s) 350, 474, 1065, 1106, 1499
Asp700I GAANNNNTTC 1 cut(s) 1500
AspLEI GCGC 1 cut(s) 1653
AspS9I GGNCC 2 cut(s) 744, 1238
AsuC2I CCSGG 1 cut(s) 684
AsuHPI GGTGA 2 cut(s) 202, 1135
AsuNHI GCTAGC 1 cut(s) 728
AvaI CYCGRG 1 cut(s) 1388
AvaII GGWCC 1 cut(s) 1238
BaeGI GKGCMC 1 cut(s) 423
BamHI GGATCC 1 cut(s) 115
BanII GRGCYC 1 cut(s) 1412
BbsI GAAGAC 3 cut(s) 53, 902, 1323
Bbv12I GWGCWC 2 cut(s) 423, 1721
BbvI GCAGC 1 cut(s) 1358
BccI CCATC 4 cut(s) 459, 847, 1241, 1485
BcnI CCSGG 1 cut(s) 684
BfaI CTAG 3 cut(s) 158, 729, 927
BfmI CTRYAG 1 cut(s) 804
BfrI CTTAAG 1 cut(s) 568
BfuAI ACCTGC 1 cut(s) 463
BisI GCNGC 1 cut(s) 1347
BlsI GCNGC 1 cut(s) 1348
Bme1390I CCNGG 1 cut(s) 684
Bme18I GGWCC 1 cut(s) 1238
BmeT110I CYCGRG 1 cut(s) 1388
BmgT120I GGNCC 2 cut(s) 744, 1238
BmiI GGNNCC 3 cut(s) 117, 746, 1301
BmrFI CCNGG 1 cut(s) 684
BmrI ACTGGG 4 cut(s) 77, 668, 909, 1036
BmsI GCATC 3 cut(s) 205, 1255, 1662
BmtI GCTAGC 1 cut(s) 732
BmuI ACTGGG 4 cut(s) 77, 668, 909, 1036
BpiI GAAGAC 3 cut(s) 53, 902, 1323
BpuEI CTTGAG 1 cut(s) 1666
BpuMI CCSGG 1 cut(s) 684
BsaAI YACGTR 1 cut(s) 1423
BsaBI GATNNNNATC 2 cut(s) 857, 1251
BsaJI CCNNGG 3 cut(s) 185, 557, 682
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 5 cut(s) 262, 599, 1177, 1247, 1597
Bse1I ACTGG 8 cut(s) 37, 72, 224, 322, 663, 904, 1031, 1718
Bse8I GATNNNNATC 2 cut(s) 857, 1251
BseDI CCNNGG 3 cut(s) 185, 557, 682
BseGI GGATG 4 cut(s) 196, 326, 541, 1252
BseJI GATNNNNATC 2 cut(s) 857, 1251
BseLI CCNNNNNNNGG 5 cut(s) 262, 599, 1177, 1247, 1597
BseMII CTCAG 3 cut(s) 148, 186, 664
BseNI ACTGG 8 cut(s) 37, 72, 224, 322, 663, 904, 1031, 1718
BseSI GKGCMC 1 cut(s) 423
BseXI GCAGC 1 cut(s) 1358
BsgI GTGCAG 1 cut(s) 1202
BshFI GGCC 5 cut(s) 250, 574, 745, 1170, 1483
BsiHKAI GWGCWC 2 cut(s) 423, 1721
BsiHKCI CYCGRG 1 cut(s) 1388
BsiSI CCGG 2 cut(s) 684, 1291
BslFI GGGAC 2 cut(s) 982, 1054
BslI CCNNNNNNNGG 5 cut(s) 262, 599, 1177, 1247, 1597
BsmFI GGGAC 2 cut(s) 982, 1054
BsmI GAATGC 1 cut(s) 1065
BsnI GGCC 5 cut(s) 250, 574, 745, 1170, 1483
BsoBI CYCGRG 1 cut(s) 1388
Bsp1286I GDGCHC 3 cut(s) 423, 1412, 1721
Bsp143I GATC 7 cut(s) 115, 163, 874, 988, 1030, 1144, 1472
BspANI GGCC 5 cut(s) 250, 574, 745, 1170, 1483
BspCNI CTCAG 3 cut(s) 147, 185, 663
BspLI GGNNCC 3 cut(s) 117, 746, 1301
BspMI ACCTGC 1 cut(s) 463
BspOI GCTAGC 1 cut(s) 732
BspPI GGATC 7 cut(s) 110, 123, 869, 983, 1038, 1139, 1467
BspTI CTTAAG 1 cut(s) 568
BsrI ACTGG 8 cut(s) 37, 72, 224, 322, 663, 904, 1031, 1718
BssECI CCNNGG 3 cut(s) 185, 557, 682
BssMI GATC 7 cut(s) 115, 163, 874, 988, 1030, 1144, 1472
BssNAI GTATAC 1 cut(s) 1642
BssT1I CCWWGG 1 cut(s) 557
Bst1107I GTATAC 1 cut(s) 1642
Bst4CI ACNGT 6 cut(s) 490, 507, 613, 982, 1235, 1369
Bst6I CTCTTC 2 cut(s) 396, 1512
BstAFI CTTAAG 1 cut(s) 568
BstBAI YACGTR 1 cut(s) 1423
BstC8I GCNNGC 2 cut(s) 730, 1397
BstDEI CTNAG 4 cut(s) 134, 172, 650, 837
BstDSI CCRYGG 1 cut(s) 185
BstENI CCTNNNNNAGG 1 cut(s) 260
BstF5I GGATG 4 cut(s) 196, 326, 541, 1252
BstHHI GCGC 1 cut(s) 1653
BstKTI GATC 7 cut(s) 118, 166, 877, 991, 1033, 1147, 1475
BstMBI GATC 7 cut(s) 115, 163, 874, 988, 1030, 1144, 1472
BstMWI GCNNNNNNNGC 1 cut(s) 1659
BstNSI RCATGY 5 cut(s) 104, 821, 1330, 1346, 1703
BstSCI CCNGG 1 cut(s) 682
BstSFI CTRYAG 1 cut(s) 804
BstSLI GKGCMC 1 cut(s) 423
BstSNI TACGTA 1 cut(s) 1423
BstV1I GCAGC 1 cut(s) 1358
BstV2I GAAGAC 3 cut(s) 53, 902, 1323
BstX2I RGATCY 3 cut(s) 115, 988, 1472
BstXI CCANNNNNNTGG 1 cut(s) 899
BstYI RGATCY 3 cut(s) 115, 988, 1472
BstZ17I GTATAC 1 cut(s) 1642
BsuRI GGCC 5 cut(s) 250, 574, 745, 1170, 1483
BtgI CCRYGG 1 cut(s) 185
BtsCI GGATG 4 cut(s) 196, 326, 541, 1252
BtsIMutI CAGTG 6 cut(s) 146, 315, 618, 1073, 1240, 1711
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 2 cut(s) 730, 1397
CfoI GCGC 1 cut(s) 1653
Cfr13I GGNCC 2 cut(s) 744, 1238
CseI GACGC 1 cut(s) 1051
Csp6I GTAC 3 cut(s) 313, 581, 1666
CviQI GTAC 3 cut(s) 313, 581, 1666
DdeI CTNAG 4 cut(s) 134, 172, 650, 837
DpnI GATC 7 cut(s) 117, 165, 876, 990, 1032, 1146, 1474
DpnII GATC 7 cut(s) 115, 163, 874, 988, 1030, 1144, 1472
DraI TTTAAA 3 cut(s) 831, 1204, 1438
EaeI YGGCCR 1 cut(s) 1481
Eam1104I CTCTTC 2 cut(s) 396, 1512
EarI CTCTTC 2 cut(s) 396, 1512
Eco105I TACGTA 1 cut(s) 1423
Eco130I CCWWGG 1 cut(s) 557
Eco147I AGGCCT 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 1412
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 1 cut(s) 1238
Eco57I CTGAAG 2 cut(s) 755, 1097
Eco88I CYCGRG 1 cut(s) 1388
EcoNI CCTNNNNNAGG 1 cut(s) 260
EcoRI GAATTC 2 cut(s) 350, 474
EcoRV GATATC 1 cut(s) 859
EcoT14I CCWWGG 1 cut(s) 557
EcoT38I GRGCYC 1 cut(s) 1412
ErhI CCWWGG 1 cut(s) 557
FalI AAGNNNNNCTT 2 cut(s) 830, 862
FaqI GGGAC 2 cut(s) 982, 1054
FauNDI CATATG 1 cut(s) 634
FblI GTMKAC 2 cut(s) 1023, 1641
Fnu4HI GCNGC 1 cut(s) 1347
FokI GGATG 4 cut(s) 183, 333, 548, 1259
FriOI GRGCYC 1 cut(s) 1412
Fsp4HI GCNGC 1 cut(s) 1347
FspBI CTAG 3 cut(s) 158, 729, 927
GlaI GCGC 1 cut(s) 1652
GluI GCNGC 1 cut(s) 1347
HaeIII GGCC 5 cut(s) 250, 574, 745, 1170, 1483
HapII CCGG 2 cut(s) 684, 1291
HgaI GACGC 1 cut(s) 1051
HhaI GCGC 1 cut(s) 1653
Hin6I GCGC 1 cut(s) 1651
HinP1I GCGC 1 cut(s) 1651
HincII GTYRAC 2 cut(s) 1024, 1188
HindII GTYRAC 2 cut(s) 1024, 1188
HindIII AAGCTT 2 cut(s) 1274, 1397
HinfI GANTC 9 cut(s) 146, 178, 379, 516, 648, 687, 907, 995, 1281
HpaII CCGG 2 cut(s) 684, 1291
HphI GGTGA 2 cut(s) 202, 1135
Hpy166II GTNNAC 8 cut(s) 30, 315, 421, 640, 1024, 1188, 1238, 1642
Hpy188I TCNGA 9 cut(s) 175, 284, 372, 416, 653, 874, 1592, 1658, 1674
Hpy8I GTNNAC 8 cut(s) 30, 315, 421, 640, 1024, 1188, 1238, 1642
HpyAV CCTTC 3 cut(s) 106, 1299, 1525
HpyCH4III ACNGT 6 cut(s) 490, 507, 613, 982, 1235, 1369
HpyCH4IV ACGT 1 cut(s) 1422
HpyF10VI GCNNNNNNNGC 1 cut(s) 1659
HpyF3I CTNAG 4 cut(s) 134, 172, 650, 837
HpySE526I ACGT 1 cut(s) 1422
HspAI GCGC 1 cut(s) 1651
Kzo9I GATC 7 cut(s) 115, 163, 874, 988, 1030, 1144, 1472
LmnI GCTCC 3 cut(s) 1299, 1376, 1716
Lsp1109I GCAGC 1 cut(s) 1358
LweI GCATC 3 cut(s) 205, 1255, 1662
MaeI CTAG 3 cut(s) 158, 729, 927
MaeII ACGT 1 cut(s) 1422
MaeIII GTNAC 2 cut(s) 613, 1363
MalI GATC 7 cut(s) 117, 165, 876, 990, 1032, 1146, 1474
MboI GATC 7 cut(s) 115, 163, 874, 988, 1030, 1144, 1472
MflI RGATCY 3 cut(s) 115, 988, 1472
MhlI GDGCHC 3 cut(s) 423, 1412, 1721
MlyI GAGTC 3 cut(s) 642, 916, 1004
MmeI TCCRAC 1 cut(s) 916
MroXI GAANNNNTTC 1 cut(s) 1500
MseI TTAA 7 cut(s) 569, 830, 933, 1014, 1203, 1437, 1677
MslI CAYNNNNRTG 6 cut(s) 105, 592, 611, 935, 1233, 1325
MspA1I CMGCKG 1 cut(s) 1349
MspCI CTTAAG 1 cut(s) 568
MspI CCGG 2 cut(s) 684, 1291
MspR9I CCNGG 1 cut(s) 684
Mva1269I GAATGC 1 cut(s) 1065
MwoI GCNNNNNNNGC 1 cut(s) 1659
NciI CCSGG 1 cut(s) 684
NdeI CATATG 1 cut(s) 634
NdeII GATC 7 cut(s) 115, 163, 874, 988, 1030, 1144, 1472
NheI GCTAGC 1 cut(s) 728
NlaIV GGNNCC 3 cut(s) 117, 746, 1301
NmuCI GTSAC 1 cut(s) 613
NspI RCATGY 5 cut(s) 104, 821, 1330, 1346, 1703
PaeR7I CTCGAG 1 cut(s) 1388
PceI AGGCCT 1 cut(s) 574
PciI ACATGT 2 cut(s) 1326, 1699
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PctI GAATGC 1 cut(s) 1065
PdmI GAANNNNTTC 1 cut(s) 1500
PfeI GAWTC 6 cut(s) 146, 178, 379, 516, 687, 1281
PflMI CCANNNNNTGG 2 cut(s) 599, 1177
PkrI GCNGC 1 cut(s) 1348
PleI GAGTC 3 cut(s) 642, 915, 1003
PpsI GAGTC 3 cut(s) 642, 915, 1003
Ppu21I YACGTR 1 cut(s) 1423
PscI ACATGT 2 cut(s) 1326, 1699
PsiI TTATAA 1 cut(s) 887
PspN4I GGNNCC 3 cut(s) 117, 746, 1301
PspPI GGNCC 2 cut(s) 744, 1238
PsuI RGATCY 3 cut(s) 115, 988, 1472
PvuII CAGCTG 1 cut(s) 1349
RsaI GTAC 3 cut(s) 314, 582, 1667
RsaNI GTAC 3 cut(s) 313, 581, 1666
RseI CAYNNNNRTG 6 cut(s) 105, 592, 611, 935, 1233, 1325
SalI GTCGAC 1 cut(s) 1022
SaqAI TTAA 7 cut(s) 569, 830, 933, 1014, 1203, 1437, 1677
SatI GCNGC 1 cut(s) 1347
Sau3AI GATC 7 cut(s) 115, 163, 874, 988, 1030, 1144, 1472
Sau96I GGNCC 2 cut(s) 744, 1238
SchI GAGTC 3 cut(s) 642, 916, 1004
ScrFI CCNGG 1 cut(s) 684
SduI GDGCHC 3 cut(s) 423, 1412, 1721
SfaNI GCATC 3 cut(s) 205, 1255, 1662
SfcI CTRYAG 1 cut(s) 804
Sfr274I CTCGAG 1 cut(s) 1388
SinI GGWCC 1 cut(s) 1238
SlaI CTCGAG 1 cut(s) 1388
SmiMI CAYNNNNRTG 6 cut(s) 105, 592, 611, 935, 1233, 1325
SmlI CTYRAG 3 cut(s) 568, 1388, 1681
SmoI CTYRAG 3 cut(s) 568, 1388, 1681
SnaBI TACGTA 1 cut(s) 1423
SseBI AGGCCT 1 cut(s) 574
SspMI CTAG 3 cut(s) 158, 729, 927
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 1 cut(s) 682
StyI CCWWGG 1 cut(s) 557
TaaI ACNGT 6 cut(s) 490, 507, 613, 982, 1235, 1369
TaiI ACGT 1 cut(s) 1425
TaqI TCGA 5 cut(s) 24, 514, 1023, 1389, 1704
TaqII GACCGA 1 cut(s) 47
TatI WGTACW 2 cut(s) 312, 1665
TfiI GAWTC 6 cut(s) 146, 178, 379, 516, 687, 1281
Tru1I TTAA 7 cut(s) 569, 830, 933, 1014, 1203, 1437, 1677
Tru9I TTAA 7 cut(s) 569, 830, 933, 1014, 1203, 1437, 1677
TscAI CASTG 6 cut(s) 146, 322, 618, 1080, 1240, 1718
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 1 cut(s) 1346
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 6 cut(s) 7, 67, 73, 453, 508, 994
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 6 cut(s) 146, 322, 618, 1080, 1240, 1718
Van91I CCANNNNNTGG 2 cut(s) 599, 1177
Vha464I CTTAAG 1 cut(s) 568
VneI GTGCAC 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 1238
XagI CCTNNNNNAGG 1 cut(s) 260
XapI RAATTY 5 cut(s) 350, 474, 1065, 1106, 1499
XceI RCATGY 5 cut(s) 104, 821, 1330, 1346, 1703
XhoI CTCGAG 1 cut(s) 1388
XmiI GTMKAC 2 cut(s) 1023, 1641
XmnI GAANNNNTTC 1 cut(s) 1500
XspI CTAG 3 cut(s) 158, 729, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.