pycom12g11620

Synaptotagmin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001241
Physical Location & Seq
Forward (+)
13907023 .. 13911612
4590 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11620.12

Sequence Viewer

Length: 1875 bp
ATGAAAGTAAAACCCTCTCCTTTTTCTGACACGTGTACTGTACAACGACGCTCTAGTTTGGCAGTTTCTGCATCCCTTTACTTTTTTGCAATTTCTGATCCTTGCATCCTCCTTTTTTTTCTTTCTTTTTTTCTTTTACATAATTCTTCCAGATATTGTATTGATGATGGATTTCTGGATTTTAATTTTCAATTTTCTGTAGGGAAAAATGAGAATTCTGAGTCTTTTATTCGATTTTATTGGATTTGGATTTGGAATTCCAATCGGCCTTGTACTGGGATTTTTTATATTTATATATTCTTAAGCCTGAAGATGCAGGATCCAATTATTAAACCCCTTGATGAGATTGATACAGGCTCTCTGATTGATCTTTTGCATGAGATTCCTCCATGGGTGAAACATCCTGACTATGAAAGAATTGACTGGTTTAACAAGGCTTTATATGATATGTGGCCTTACCTTGATAAGGCAATATGCGGTATCATACGAAGCGCCACGAAGCCTATATTTGCAGAGTACATTGGAAAGTATCAGATAAAATCGATCGAATTTGAGACCCTGACTCTCGGAAGCCTTCCTCCTACAATTTACGGCATGAGAGTGCATGAAACCACTGAAAACGAACTGATATTTGAGCCTGCAATTAGATGGGCAGGAAATCCCCACATAACAGTGGTGTTGAAACTGTTGCCGTTTCGATTTACAGCACAGTTGGTGGATATCCAAATATTTGCAGCACCAAGGATAATTTTGAGGCCTCTGGTGCCATCATTCCCATGTTTTGCAAACGTAGTAGTGAGTTTGATAGATAAGCCACATGTGGATTTTGGACTCAAAATTCAGGGAGGGGATGTAATGGCAATCCCTGGTTTGTACCAATTCATCCAGGAATTTATAAAAAGACAAGTTGCTAGCCTTTATTTATGGCCCCAAACTCTGGACATACCAATTCTTGATAGTTCCCTATCAGGGACAATAAAGAAGCCAGTAGGGATACTACATGTGAAGGTTGTAAAAGCACTCAAACTCTTGAAGATGGACATCATTGGAACATCTGACCCTTATGTTAAAATCAAACTAAGTGGGGAGAGGCTTCCTTCAAAGAAAACAACCATTAAGATGAGGAACCTTAACCCTGAATGGAATGAGAGTTTCAGGCTTACAGTTAAAGATCCCCAGACCCAAATCCTTCAATTTGATCTTTATGATTGGGAACAGATTGGAACACATGATTTTTTAGGAATGCAAATTGTTCCACTGAAGGTGCTTATCCCACATGAGACGCAAGAATTAACTCTTGATTTGGTGAAGAACACAAACCCTTATGATCCTGTAAACAAGAAAGGGAGAGGCCACCTCATAGTGGAGTTGACATACAATCCTTTTATAGATGACCATGGAAACTACAGTGGACCTTTGGATGGAAAAGTTGCAGGGGAAAAAAGTCATCAAAGTAAGAGCCGAAAGGGATCCAAGGATTTTTCTTGCTTTTCGTCGCTAGAAGCAGCCGGTTTGCTTTCGGTTACAGTCCAGGGAGCTGAAGATGTTTCTGGCAAGAATCGCAGTAACCCTAACCCTTATGCTTTGGTTAGCTTTGGAGAAGAAACTAAAATAACCAAGATGCTTAGGAAAACCCAGTGTCCAAGTTGGAATGAAGAATTCCAGTTTGTGATAGAAGATGCTTCACTGAAGGAGAAGATCCATATTGAAGTAATGAGTAAGAAAAGATTTGGTTTTCGTCGACAGGAGTCGTTGGGGTACATTGACATCAATTTTAGTGATGTGGTATACAATGGACGCATTAGAGGGAAGTACAATTTGATCGACTCAAAAAATGGAGTTATACATATTGAGACGCAGTGGAATTCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 SMP_LBD

Protein Analysis

625

Amino Acids

71.45

Weight (kDa)

8.2

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 896
AccB1I GGYRCC 1 cut(s) 763
AccB7I CCANNNNNTGG 1 cut(s) 937
AccI GTMKAC 2 cut(s) 1741, 1788
AciI CCGC 1 cut(s) 477
AclWI GGATC 8 cut(s) 92, 314, 327, 1166, 1322, 1464, 1477, 1693
AcsI RAATTY 7 cut(s) 214, 256, 548, 837, 890, 1658, 1864
AcuI CTGAAG 4 cut(s) 329, 1280, 1560, 1709
AcvI CACGTG 1 cut(s) 33
AfaI GTAC 7 cut(s) 37, 42, 274, 518, 875, 1760, 1814
AfiI CCNNNNNNNGG 6 cut(s) 275, 466, 937, 969, 1363, 1421
AflII CTTAAG 1 cut(s) 301
AflIII ACRYGT 4 cut(s) 30, 32, 817, 1000
AgsI TTSAA 6 cut(s) 191, 682, 1033, 1101, 1193, 1709
AjnI CCWGG 3 cut(s) 865, 885, 1530
AluBI AGCT 2 cut(s) 1538, 1593
AluI AGCT 2 cut(s) 1538, 1593
Alw26I GTCTC 3 cut(s) 548, 1274, 1847
AlwI GGATC 8 cut(s) 92, 314, 327, 1166, 1322, 1464, 1477, 1693
AlwNI CAGNNNCTG 1 cut(s) 68
AoxI GGCC 5 cut(s) 266, 452, 755, 926, 1351
ApeKI GCWGC 2 cut(s) 734, 1505
ApoI RAATTY 7 cut(s) 214, 256, 548, 837, 890, 1658, 1864
AspLEI GCGC 1 cut(s) 494
AspS9I GGNCC 2 cut(s) 927, 1412
AsuHPI GGTGA 2 cut(s) 406, 1318
AsuNHI GCTAGC 1 cut(s) 911
AvaII GGWCC 1 cut(s) 1412
BamHI GGATCC 2 cut(s) 319, 1469
BanI GGYRCC 1 cut(s) 763
BbrPI CACGTG 1 cut(s) 33
BbvI GCAGC 2 cut(s) 746, 1517
BccI CCATC 5 cut(s) 161, 642, 775, 1030, 1415
BceAI ACGGC 2 cut(s) 607, 676
BciT130I CCWGG 3 cut(s) 867, 887, 1532
BciVI GTATCC 1 cut(s) 987
BcoDI GTCTC 3 cut(s) 548, 1274, 1847
BfaI CTAG 3 cut(s) 54, 912, 1499
BfmI CTRYAG 2 cut(s) 198, 1405
BfoI RGCGCY 1 cut(s) 495
BfrI CTTAAG 1 cut(s) 301
BfuI GTATCC 1 cut(s) 987
BisI GCNGC 2 cut(s) 735, 1506
BlsI GCNGC 2 cut(s) 736, 1507
Bme1390I CCNGG 3 cut(s) 867, 887, 1532
Bme18I GGWCC 1 cut(s) 1412
BmgT120I GGNCC 2 cut(s) 927, 1412
BmiI GGNNCC 5 cut(s) 321, 765, 929, 1127, 1471
BmrFI CCNGG 3 cut(s) 867, 887, 1532
BmrI ACTGGG 2 cut(s) 285, 1630
BmsI GCATC 5 cut(s) 80, 114, 303, 1611, 1669
BmtI GCTAGC 1 cut(s) 915
BmuI ACTGGG 2 cut(s) 285, 1630
BoxI GACNNNNGTC 1 cut(s) 1747
BplI GAGNNNNNCTC 2 cut(s) 1341, 1373
Bpu10I CCTNAGC 1 cut(s) 1625
Bsa29I ATCGAT 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 33
BsaBI GATNNNNATC 1 cut(s) 1040
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 6 cut(s) 389, 740, 865, 1396, 1473, 1531
BsaXI ACNNNNNCTCC 2 cut(s) 837, 867
Bsc4I CCNNNNNNNGG 6 cut(s) 275, 466, 937, 969, 1363, 1421
Bse118I RCCGGY 1 cut(s) 1508
Bse1I ACTGG 5 cut(s) 280, 428, 986, 1636, 1663
Bse8I GATNNNNATC 1 cut(s) 1040
BseBI CCWGG 3 cut(s) 867, 887, 1532
BseCI ATCGAT 1 cut(s) 542
BseDI CCNNGG 6 cut(s) 389, 740, 865, 1396, 1473, 1531
BseGI GGATG 6 cut(s) 71, 105, 400, 856, 882, 1426
BseJI GATNNNNATC 1 cut(s) 1040
BseLI CCNNNNNNNGG 6 cut(s) 275, 466, 937, 969, 1363, 1421
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 5 cut(s) 280, 428, 986, 1636, 1663
BseXI GCAGC 2 cut(s) 746, 1517
Bsh1285I CGRYCG 1 cut(s) 546
BshFI GGCC 5 cut(s) 268, 454, 757, 928, 1353
BshNI GGYRCC 1 cut(s) 763
BshVI ATCGAT 1 cut(s) 542
BsiEI CGRYCG 1 cut(s) 546
BsiSI CCGG 1 cut(s) 1509
BslFI GGGAC 1 cut(s) 985
BslI CCNNNNNNNGG 6 cut(s) 275, 466, 937, 969, 1363, 1421
BsmAI GTCTC 3 cut(s) 548, 1274, 1847
BsmBI CGTCTC 2 cut(s) 1274, 1847
BsmFI GGGAC 1 cut(s) 985
BsmI GAATGC 1 cut(s) 1248
BsnI GGCC 5 cut(s) 268, 454, 757, 928, 1353
Bso31I GGTCTC 1 cut(s) 548
Bsp1407I TGTACA 1 cut(s) 40
Bsp19I CCATGG 2 cut(s) 389, 1396
BspACI CCGC 1 cut(s) 477
BspANI GGCC 5 cut(s) 268, 454, 757, 928, 1353
BspCNI CTCAG 1 cut(s) 211
BspDI ATCGAT 1 cut(s) 542
BspLI GGNNCC 5 cut(s) 321, 765, 929, 1127, 1471
BspOI GCTAGC 1 cut(s) 915
BspPI GGATC 8 cut(s) 92, 314, 327, 1166, 1322, 1464, 1477, 1693
BspT107I GGYRCC 1 cut(s) 763
BspTI CTTAAG 1 cut(s) 301
BspTNI GGTCTC 1 cut(s) 548
BsrFI RCCGGY 1 cut(s) 1508
BsrGI TGTACA 1 cut(s) 40
BsrI ACTGG 5 cut(s) 280, 428, 986, 1636, 1663
BssAI RCCGGY 1 cut(s) 1508
BssECI CCNNGG 6 cut(s) 389, 740, 865, 1396, 1473, 1531
BssNAI GTATAC 1 cut(s) 1789
BssT1I CCWWGG 4 cut(s) 389, 740, 1396, 1473
Bst1107I GTATAC 1 cut(s) 1789
Bst2UI CCWGG 3 cut(s) 867, 887, 1532
Bst4CI ACNGT 7 cut(s) 40, 673, 687, 711, 1165, 1409, 1528
BstAFI CTTAAG 1 cut(s) 301
BstAPI GCANNNNNTGC 1 cut(s) 68
BstAUI TGTACA 1 cut(s) 40
BstBAI YACGTR 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 639, 913
BstDEI CTNAG 3 cut(s) 219, 1079, 1625
BstDSI CCRYGG 2 cut(s) 389, 1396
BstENI CCTNNNNNAGG 1 cut(s) 464
BstF5I GGATG 6 cut(s) 71, 105, 400, 856, 882, 1426
BstH2I RGCGCY 1 cut(s) 495
BstHHI GCGC 1 cut(s) 494
BstMAI GTCTC 3 cut(s) 548, 1274, 1847
BstMCI CGRYCG 1 cut(s) 546
BstMWI GCNNNNNNNGC 3 cut(s) 68, 763, 1560
BstNI CCWGG 3 cut(s) 867, 887, 1532
BstNSI RCATGY 2 cut(s) 821, 1004
BstPAI GACNNNNGTC 1 cut(s) 1747
BstSCI CCNGG 3 cut(s) 865, 885, 1530
BstSFI CTRYAG 2 cut(s) 198, 1405
BstV1I GCAGC 2 cut(s) 746, 1517
BstX2I RGATCY 4 cut(s) 319, 1171, 1469, 1698
BstYI RGATCY 4 cut(s) 319, 1171, 1469, 1698
BstZ17I GTATAC 1 cut(s) 1789
Bsu15I ATCGAT 1 cut(s) 542
BsuI GTATCC 1 cut(s) 987
BsuRI GGCC 5 cut(s) 268, 454, 757, 928, 1353
BsuTUI ATCGAT 1 cut(s) 542
BtgI CCRYGG 2 cut(s) 389, 1396
BtsCI GGATG 6 cut(s) 71, 105, 400, 856, 882, 1426
BtsI GCAGTG 1 cut(s) 1865
BtsIMutI CAGTG 7 cut(s) 612, 678, 1256, 1414, 1643, 1685, 1865
Cac8I GCNNGC 2 cut(s) 639, 913
CaiI CAGNNNCTG 1 cut(s) 68
CfoI GCGC 1 cut(s) 494
Cfr10I RCCGGY 1 cut(s) 1508
Cfr13I GGNCC 2 cut(s) 927, 1412
ClaI ATCGAT 1 cut(s) 542
CseI GACGC 4 cut(s) 57, 1291, 1806, 1864
Csp6I GTAC 7 cut(s) 36, 41, 273, 517, 874, 1759, 1813
CspCI CAANNNNNGTGG 2 cut(s) 1063, 1098
CviQI GTAC 7 cut(s) 36, 41, 273, 517, 874, 1759, 1813
DdeI CTNAG 3 cut(s) 219, 1079, 1625
Eco130I CCWWGG 4 cut(s) 389, 740, 1396, 1473
Eco147I AGGCCT 1 cut(s) 757
Eco31I GGTCTC 1 cut(s) 548
Eco32I GATATC 1 cut(s) 721
Eco47I GGWCC 1 cut(s) 1412
Eco57I CTGAAG 4 cut(s) 329, 1280, 1560, 1709
Eco72I CACGTG 1 cut(s) 33
EcoNI CCTNNNNNAGG 1 cut(s) 464
EcoRI GAATTC 4 cut(s) 214, 256, 1658, 1864
EcoRII CCWGG 3 cut(s) 865, 885, 1530
EcoRV GATATC 1 cut(s) 721
EcoT14I CCWWGG 4 cut(s) 389, 740, 1396, 1473
ErhI CCWWGG 4 cut(s) 389, 740, 1396, 1473
Esp3I CGTCTC 2 cut(s) 1274, 1847
FaqI GGGAC 1 cut(s) 985
FblI GTMKAC 2 cut(s) 1741, 1788
Fnu4HI GCNGC 2 cut(s) 735, 1506
FokI GGATG 6 cut(s) 58, 92, 387, 863, 869, 1433
Fsp4HI GCNGC 2 cut(s) 735, 1506
FspBI CTAG 3 cut(s) 54, 912, 1499
GlaI GCGC 1 cut(s) 493
GluI GCNGC 2 cut(s) 735, 1506
HaeII RGCGCY 1 cut(s) 495
HaeIII GGCC 5 cut(s) 268, 454, 757, 928, 1353
HapII CCGG 1 cut(s) 1509
HgaI GACGC 4 cut(s) 57, 1291, 1806, 1864
HhaI GCGC 1 cut(s) 494
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HincII GTYRAC 2 cut(s) 1371, 1742
HindII GTYRAC 2 cut(s) 1371, 1742
HinfI GANTC 7 cut(s) 221, 382, 562, 831, 1558, 1748, 1826
HpaII CCGG 1 cut(s) 1509
HphI GGTGA 2 cut(s) 406, 1318
Hpy166II GTNNAC 6 cut(s) 36, 1336, 1371, 1412, 1742, 1789
Hpy188I TCNGA 7 cut(s) 28, 97, 220, 363, 534, 569, 1057
Hpy188III TCNNGA 7 cut(s) 150, 176, 404, 938, 953, 1030, 1298
Hpy8I GTNNAC 6 cut(s) 36, 1336, 1371, 1412, 1742, 1789
Hpy99I CGWCG 3 cut(s) 51, 1498, 1743
HpyAV CCTTC 6 cut(s) 584, 1000, 1107, 1199, 1255, 1684
HpyCH4III ACNGT 7 cut(s) 40, 673, 687, 711, 1165, 1409, 1528
HpyCH4IV ACGT 2 cut(s) 32, 789
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 763, 1560
HpyF3I CTNAG 3 cut(s) 219, 1079, 1625
HpySE526I ACGT 2 cut(s) 32, 789
HspAI GCGC 1 cut(s) 492
LmnI GCTCC 1 cut(s) 1535
Lsp1109I GCAGC 2 cut(s) 746, 1517
LweI GCATC 5 cut(s) 80, 114, 303, 1611, 1669
MaeI CTAG 3 cut(s) 54, 912, 1499
MaeII ACGT 2 cut(s) 32, 789
MaeIII GTNAC 2 cut(s) 1522, 1565
MboII GAAGA 9 cut(s) 138, 322, 1045, 1321, 1553, 1613, 1667, 1688, 1708
MflI RGATCY 4 cut(s) 319, 1171, 1469, 1698
MlyI GAGTC 5 cut(s) 230, 556, 825, 1757, 1820
MmeI TCCRAC 1 cut(s) 1628
MseI TTAA 9 cut(s) 183, 302, 330, 429, 1068, 1116, 1131, 1167, 1292
MslI CAYNNNNRTG 4 cut(s) 599, 671, 775, 1118
MspCI CTTAAG 1 cut(s) 301
MspI CCGG 1 cut(s) 1509
MspR9I CCNGG 3 cut(s) 867, 887, 1532
Mva1269I GAATGC 1 cut(s) 1248
MvaI CCWGG 3 cut(s) 867, 887, 1532
MwoI GCNNNNNNNGC 3 cut(s) 68, 763, 1560
NcoI CCATGG 2 cut(s) 389, 1396
NheI GCTAGC 1 cut(s) 911
NlaIV GGNNCC 5 cut(s) 321, 765, 929, 1127, 1471
NspI RCATGY 2 cut(s) 821, 1004
PceI AGGCCT 1 cut(s) 757
PciI ACATGT 2 cut(s) 817, 1000
PctI GAATGC 1 cut(s) 1248
PfeI GAWTC 2 cut(s) 382, 1558
PflMI CCANNNNNTGG 1 cut(s) 937
PfoI TCCNGGA 1 cut(s) 885
PkrI GCNGC 2 cut(s) 736, 1507
Ple19I CGATCG 1 cut(s) 546
PleI GAGTC 5 cut(s) 229, 556, 825, 1756, 1820
PmaCI CACGTG 1 cut(s) 33
PmlI CACGTG 1 cut(s) 33
PpsI GAGTC 5 cut(s) 229, 556, 825, 1756, 1820
Ppu21I YACGTR 1 cut(s) 33
PscI ACATGT 2 cut(s) 817, 1000
PshAI GACNNNNGTC 1 cut(s) 1747
PsiI TTATAA 1 cut(s) 896
Psp6I CCWGG 3 cut(s) 865, 885, 1530
PspCI CACGTG 1 cut(s) 33
PspGI CCWGG 3 cut(s) 865, 885, 1530
PspN4I GGNNCC 5 cut(s) 321, 765, 929, 1127, 1471
PspPI GGNCC 2 cut(s) 927, 1412
PstNI CAGNNNCTG 1 cut(s) 68
PsuI RGATCY 4 cut(s) 319, 1171, 1469, 1698
PvuI CGATCG 1 cut(s) 546
RsaI GTAC 7 cut(s) 37, 42, 274, 518, 875, 1760, 1814
RsaNI GTAC 7 cut(s) 36, 41, 273, 517, 874, 1759, 1813
RseI CAYNNNNRTG 4 cut(s) 599, 671, 775, 1118
SalI GTCGAC 1 cut(s) 1740
SaqAI TTAA 9 cut(s) 183, 302, 330, 429, 1068, 1116, 1131, 1167, 1292
SatI GCNGC 2 cut(s) 735, 1506
Sau96I GGNCC 2 cut(s) 927, 1412
SchI GAGTC 5 cut(s) 230, 556, 825, 1757, 1820
ScrFI CCNGG 3 cut(s) 867, 887, 1532
SfaNI GCATC 5 cut(s) 80, 114, 303, 1611, 1669
SfcI CTRYAG 2 cut(s) 198, 1405
SinI GGWCC 1 cut(s) 1412
SmiMI CAYNNNNRTG 4 cut(s) 599, 671, 775, 1118
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
SseBI AGGCCT 1 cut(s) 757
SsiI CCGC 1 cut(s) 477
SspI AATATT 1 cut(s) 729
SspMI CTAG 3 cut(s) 54, 912, 1499
StuI AGGCCT 1 cut(s) 757
StyD4I CCNGG 3 cut(s) 865, 885, 1530
StyI CCWWGG 4 cut(s) 389, 740, 1396, 1473
TaaI ACNGT 7 cut(s) 40, 673, 687, 711, 1165, 1409, 1528
TaiI ACGT 2 cut(s) 35, 792
TaqI TCGA 6 cut(s) 232, 542, 546, 697, 1741, 1824
TatI WGTACW 5 cut(s) 35, 40, 272, 516, 1812
TfiI GAWTC 2 cut(s) 382, 1558
Tru1I TTAA 9 cut(s) 183, 302, 330, 429, 1068, 1116, 1131, 1167, 1292
Tru9I TTAA 9 cut(s) 183, 302, 330, 429, 1068, 1116, 1131, 1167, 1292
TscAI CASTG 7 cut(s) 619, 678, 1263, 1414, 1643, 1692, 1865
TseI GCWGC 2 cut(s) 734, 1505
TspDTI ATGAA 5 cut(s) 17, 426, 621, 871, 1668
TspRI CASTG 7 cut(s) 619, 678, 1263, 1414, 1643, 1692, 1865
Van91I CCANNNNNTGG 1 cut(s) 937
Vha464I CTTAAG 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 1412
XagI CCTNNNNNAGG 1 cut(s) 464
XapI RAATTY 7 cut(s) 214, 256, 548, 837, 890, 1658, 1864
XceI RCATGY 2 cut(s) 821, 1004
XmiI GTMKAC 2 cut(s) 1741, 1788
XspI CTAG 3 cut(s) 54, 912, 1499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.