MD12G1117500.v1.1

Synaptotagmin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001241
Physical Location & Seq
Forward (+)
18772378 .. 18776780
4403 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1117500.v1.1.491

Sequence Viewer

Length: 1668 bp
ATGAGAATTCTGAGTCTTTTATCCGATTTTATTGGATTTGGATTTGGAATTCCAATCGGCCTTGTACTGGGATTTTTCATATTTATATACTCTAAGCCTGAAGATGTGAAGGATCCAATTATTAAACCCCTTGATGAGATTGATACAGGCTCTCTGATTGATCTTTTGCATGAGATTCCTCCATGGGTGAAACATCCTGACTATGAAAGAATTGACTGGTTTAACAAGGCTTTATATGATATGTGGCCTTACCTTGATAAGGCAATATGCGGTATCATACGAAGCACCACGAAGCCTATATTTGCAGAGTACATTGGAAAGTATCAGATAAAGCCGATCGAATTCGAGACCCTGACTCTCGGAAGCCTTCCTCCTACAATTTACGGCATGAGAGTTCATGAAACCACTGAAAACGAACTGATATTTGAGCCTGCAATTAGATGGGCAGGAAATCCCCACATAACAGTGGTGTTGAAACTGTTGCCGTTTCGATTTACAGCACAGTTGGTGGATATCCAAATATTTGCAGCACCAAGGATAATTTTGAGGCCTCTGGTGCCATCATTCCCATGTTTTGGAAACATAGTAGTGAGTTTGATAGATAAGCCACATGTGGATTTTGGACTCAAAATTCAGGGAGGGGATGTGATGGCAATTCCTGGTTTGTACCAATTCATCCAGGAATTTATAAAAAGACAAGTTGCTAGCCTTTATTTATGGCCCCAAACTCTGGACATACCAATTCTTGATAGTTCCCTATCAGGGACAATAAAGAAGCCAGTAGGGATACTACATGTGAAGGTTGTAAGAGCACTCAAACTCTTGAAGATGGACATCATTGGAACATCTGACCCTTATGTTAAAATTAAACTAAGTGGGGAGAGGCTTCCTTCAAAGAAAACTACCATTAAGATGAGGAACCTTAACCCTGAATGGAATGAGAGTTTCAGGCTTACAGTTAAAGATCCCCAGACCCAAATCCTTCAATTTGATCTTTATGATTGGGAACAGATTGGAACACATGATTTTTTAGGAATGCAAATTGTTCCACTGAAGGTGCTTACCCCACATGAGACGAAAGAATTAACTCTTGATTTGGTGAAGAACACAAACCCTTATGATCCTGTAAACAAGAAAGGGAGAGGCCACCTCATAGTGGAGTTGACATACAATCCTTTTATAGATGACCATGGAAACTGCAGTGGACCTTTGGATGGAAAAGTTGCTGGAGAAAAAAGTCACCAAAGTAGGAGCCGAAAGGGATCCATGGATTTTTCTTGCTTTTCGTCGCTAGAAGCAGCCGGTTTGCTTTCGGTTACAGTCCAAGGAGCTGAAGATGTTTCCGGCAAGAATCGCAGTAACCCTAACCCTTATGCTTTGGTCAGCTTTGGAGAAGAAACTAAGATAACCAAGATGCTTAGGAAAACCCAGTGTCCAAGTTGGAATGAAGAATTCCAGTTTGTGATAGAAGATGCTTCACTGAAGGAGAAGATCCATATTGAAGTAATGAGTAAGAAAAGATTTGGTTTTCGTCGACAGGAGTCGTTGGGGTACATTGACATCAATTTTAGTGATGTGGTATACAATGGACGCATTAGAGGGAAGTACAATTTGATCAACTCAAAAAATGGAGTTATACATATTGAGACGCAGTGGAATTCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 SMP_LBD

Protein Analysis

556

Amino Acids

63.09

Weight (kDa)

8.06

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMP_LBD PF17047 67 - 248 1.2e-13 Synaptotagmin-like mitochondrial-lipid-binding domain
C2 PF00168 262 - 363 4e-24 C2 domain
C2 PF00168 435 - 538 5.1e-16 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 689
AccB1I GGYRCC 1 cut(s) 556
AccB7I CCANNNNNTGG 2 cut(s) 575, 730
AccI GTMKAC 2 cut(s) 1534, 1581
AciI CCGC 1 cut(s) 270
AclWI GGATC 7 cut(s) 107, 120, 959, 1115, 1257, 1270, 1486
AcsI RAATTY 7 cut(s) 6, 48, 341, 630, 683, 1451, 1657
AcuI CTGAAG 4 cut(s) 120, 1073, 1353, 1502
AfaI GTAC 5 cut(s) 66, 311, 668, 1553, 1607
AfiI CCNNNNNNNGG 7 cut(s) 67, 259, 575, 730, 762, 1156, 1214
AflIII ACRYGT 2 cut(s) 610, 793
AgsI TTSAA 5 cut(s) 475, 826, 894, 986, 1502
AjnI CCWGG 2 cut(s) 658, 678
AluBI AGCT 2 cut(s) 1331, 1386
AluI AGCT 2 cut(s) 1331, 1386
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 3 cut(s) 341, 1067, 1640
AlwI GGATC 7 cut(s) 107, 120, 959, 1115, 1257, 1270, 1486
AoxI GGCC 5 cut(s) 58, 245, 548, 719, 1144
ApeKI GCWGC 2 cut(s) 527, 1298
ApoI RAATTY 7 cut(s) 6, 48, 341, 630, 683, 1451, 1657
AspS9I GGNCC 2 cut(s) 720, 1205
AsuHPI GGTGA 3 cut(s) 199, 1111, 1232
AsuNHI GCTAGC 1 cut(s) 704
AvaII GGWCC 1 cut(s) 1205
BamHI GGATCC 2 cut(s) 112, 1262
BanI GGYRCC 1 cut(s) 556
Bbv12I GWGCWC 1 cut(s) 814
BbvI GCAGC 2 cut(s) 539, 1310
BccI CCATC 5 cut(s) 435, 568, 643, 823, 1208
BceAI ACGGC 2 cut(s) 400, 469
BciT130I CCWGG 2 cut(s) 660, 680
BciVI GTATCC 1 cut(s) 780
BclI TGATCA 1 cut(s) 1614
BcoDI GTCTC 3 cut(s) 341, 1067, 1640
BfaI CTAG 2 cut(s) 705, 1292
BfmI CTRYAG 1 cut(s) 1198
BfuI GTATCC 1 cut(s) 780
BisI GCNGC 2 cut(s) 528, 1299
BlsI GCNGC 2 cut(s) 529, 1300
Bme1390I CCNGG 2 cut(s) 660, 680
Bme18I GGWCC 1 cut(s) 1205
BmgT120I GGNCC 2 cut(s) 720, 1205
BmiI GGNNCC 6 cut(s) 114, 558, 722, 920, 1253, 1264
BmrFI CCNGG 2 cut(s) 660, 680
BmrI ACTGGG 2 cut(s) 77, 1423
BmsI GCATC 2 cut(s) 1404, 1462
BmtI GCTAGC 1 cut(s) 708
BmuI ACTGGG 2 cut(s) 77, 1423
BoxI GACNNNNGTC 1 cut(s) 1540
BplI GAGNNNNNCTC 2 cut(s) 1134, 1166
BpmI CTGGAG 1 cut(s) 1248
Bpu10I CCTNAGC 1 cut(s) 1418
BsaBI GATNNNNATC 1 cut(s) 833
BsaI GGTCTC 1 cut(s) 341
BsaJI CCNNGG 5 cut(s) 182, 533, 1189, 1266, 1324
BsaXI ACNNNNNCTCC 2 cut(s) 630, 660
Bsc4I CCNNNNNNNGG 7 cut(s) 67, 259, 575, 730, 762, 1156, 1214
Bse118I RCCGGY 1 cut(s) 1301
Bse1I ACTGG 5 cut(s) 72, 221, 779, 1429, 1456
Bse8I GATNNNNATC 1 cut(s) 833
BseBI CCWGG 2 cut(s) 660, 680
BseDI CCNNGG 5 cut(s) 182, 533, 1189, 1266, 1324
BseGI GGATG 4 cut(s) 193, 649, 675, 1219
BseJI GATNNNNATC 1 cut(s) 833
BseLI CCNNNNNNNGG 7 cut(s) 67, 259, 575, 730, 762, 1156, 1214
BseNI ACTGG 5 cut(s) 72, 221, 779, 1429, 1456
BseXI GCAGC 2 cut(s) 539, 1310
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 5 cut(s) 60, 247, 550, 721, 1146
BshNI GGYRCC 1 cut(s) 556
BsiEI CGRYCG 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 814
BsiSI CCGG 2 cut(s) 1302, 1344
BslFI GGGAC 1 cut(s) 778
BslI CCNNNNNNNGG 7 cut(s) 67, 259, 575, 730, 762, 1156, 1214
BsmAI GTCTC 3 cut(s) 341, 1067, 1640
BsmBI CGTCTC 2 cut(s) 1067, 1640
BsmFI GGGAC 1 cut(s) 778
BsmI GAATGC 1 cut(s) 1041
BsnI GGCC 5 cut(s) 60, 247, 550, 721, 1146
Bso31I GGTCTC 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 814
Bsp143I GATC 9 cut(s) 112, 160, 336, 964, 991, 1120, 1262, 1491, 1614
Bsp19I CCATGG 3 cut(s) 182, 1189, 1266
BspACI CCGC 1 cut(s) 270
BspANI GGCC 5 cut(s) 60, 247, 550, 721, 1146
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 6 cut(s) 114, 558, 722, 920, 1253, 1264
BspMAI CTGCAG 1 cut(s) 1202
BspOI GCTAGC 1 cut(s) 708
BspPI GGATC 7 cut(s) 107, 120, 959, 1115, 1257, 1270, 1486
BspT107I GGYRCC 1 cut(s) 556
BspTNI GGTCTC 1 cut(s) 341
BsrFI RCCGGY 1 cut(s) 1301
BsrI ACTGG 5 cut(s) 72, 221, 779, 1429, 1456
BssAI RCCGGY 1 cut(s) 1301
BssECI CCNNGG 5 cut(s) 182, 533, 1189, 1266, 1324
BssMI GATC 9 cut(s) 112, 160, 336, 964, 991, 1120, 1262, 1491, 1614
BssNAI GTATAC 1 cut(s) 1582
BssT1I CCWWGG 5 cut(s) 182, 533, 1189, 1266, 1324
Bst1107I GTATAC 1 cut(s) 1582
Bst2UI CCWGG 2 cut(s) 660, 680
Bst4CI ACNGT 5 cut(s) 466, 480, 504, 958, 1321
BstC8I GCNNGC 2 cut(s) 432, 706
BstDEI CTNAG 5 cut(s) 11, 93, 872, 1401, 1418
BstDSI CCRYGG 3 cut(s) 182, 1189, 1266
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 4 cut(s) 193, 649, 675, 1219
BstKTI GATC 9 cut(s) 115, 163, 339, 967, 994, 1123, 1265, 1494, 1617
BstMAI GTCTC 3 cut(s) 341, 1067, 1640
BstMBI GATC 9 cut(s) 112, 160, 336, 964, 991, 1120, 1262, 1491, 1614
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 2 cut(s) 556, 1353
BstNI CCWGG 2 cut(s) 660, 680
BstNSI RCATGY 2 cut(s) 614, 797
BstPAI GACNNNNGTC 1 cut(s) 1540
BstSCI CCNGG 2 cut(s) 658, 678
BstSFI CTRYAG 1 cut(s) 1198
BstV1I GCAGC 2 cut(s) 539, 1310
BstX2I RGATCY 4 cut(s) 112, 964, 1262, 1491
BstYI RGATCY 4 cut(s) 112, 964, 1262, 1491
BstZ17I GTATAC 1 cut(s) 1582
BsuI GTATCC 1 cut(s) 780
BsuRI GGCC 5 cut(s) 60, 247, 550, 721, 1146
BtgI CCRYGG 3 cut(s) 182, 1189, 1266
BtsCI GGATG 4 cut(s) 193, 649, 675, 1219
BtsI GCAGTG 2 cut(s) 1207, 1658
BtsIMutI CAGTG 7 cut(s) 405, 471, 1049, 1207, 1436, 1478, 1658
Cac8I GCNNGC 2 cut(s) 432, 706
CciI TCATGA 1 cut(s) 397
Cfr10I RCCGGY 1 cut(s) 1301
Cfr13I GGNCC 2 cut(s) 720, 1205
CseI GACGC 2 cut(s) 1599, 1657
Csp6I GTAC 5 cut(s) 65, 310, 667, 1552, 1606
CviQI GTAC 5 cut(s) 65, 310, 667, 1552, 1606
DdeI CTNAG 5 cut(s) 11, 93, 872, 1401, 1418
DpnI GATC 9 cut(s) 114, 162, 338, 966, 993, 1122, 1264, 1493, 1616
DpnII GATC 9 cut(s) 112, 160, 336, 964, 991, 1120, 1262, 1491, 1614
Eco130I CCWWGG 5 cut(s) 182, 533, 1189, 1266, 1324
Eco147I AGGCCT 1 cut(s) 550
Eco31I GGTCTC 1 cut(s) 341
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 1205
Eco57I CTGAAG 4 cut(s) 120, 1073, 1353, 1502
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoRI GAATTC 5 cut(s) 6, 48, 341, 1451, 1657
EcoRII CCWGG 2 cut(s) 658, 678
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 5 cut(s) 182, 533, 1189, 1266, 1324
ErhI CCWWGG 5 cut(s) 182, 533, 1189, 1266, 1324
Esp3I CGTCTC 2 cut(s) 1067, 1640
FaqI GGGAC 1 cut(s) 778
FbaI TGATCA 1 cut(s) 1614
FblI GTMKAC 2 cut(s) 1534, 1581
Fnu4HI GCNGC 2 cut(s) 528, 1299
FokI GGATG 4 cut(s) 180, 656, 662, 1226
Fsp4HI GCNGC 2 cut(s) 528, 1299
FspBI CTAG 2 cut(s) 705, 1292
GluI GCNGC 2 cut(s) 528, 1299
GsuI CTGGAG 1 cut(s) 1248
HaeIII GGCC 5 cut(s) 60, 247, 550, 721, 1146
HapII CCGG 2 cut(s) 1302, 1344
HgaI GACGC 2 cut(s) 1599, 1657
HincII GTYRAC 2 cut(s) 1164, 1535
HindII GTYRAC 2 cut(s) 1164, 1535
HinfI GANTC 6 cut(s) 13, 175, 355, 624, 1351, 1541
HpaII CCGG 2 cut(s) 1302, 1344
HphI GGTGA 3 cut(s) 199, 1111, 1232
Hpy166II GTNNAC 5 cut(s) 1129, 1164, 1205, 1535, 1582
Hpy188I TCNGA 6 cut(s) 12, 25, 156, 327, 362, 850
Hpy188III TCNNGA 7 cut(s) 197, 346, 398, 731, 746, 823, 1091
Hpy8I GTNNAC 5 cut(s) 1129, 1164, 1205, 1535, 1582
Hpy99I CGWCG 2 cut(s) 1291, 1536
HpyAV CCTTC 7 cut(s) 103, 377, 793, 900, 992, 1048, 1477
HpyCH4III ACNGT 5 cut(s) 466, 480, 504, 958, 1321
HpyCH4V TGCA 6 cut(s) 169, 305, 434, 527, 1039, 1200
HpyF10VI GCNNNNNNNGC 2 cut(s) 556, 1353
HpyF3I CTNAG 5 cut(s) 11, 93, 872, 1401, 1418
Ksp22I TGATCA 1 cut(s) 1614
Kzo9I GATC 9 cut(s) 112, 160, 336, 964, 991, 1120, 1262, 1491, 1614
LmnI GCTCC 2 cut(s) 1251, 1328
Lsp1109I GCAGC 2 cut(s) 539, 1310
LweI GCATC 2 cut(s) 1404, 1462
MaeI CTAG 2 cut(s) 705, 1292
MaeIII GTNAC 3 cut(s) 1238, 1315, 1358
MalI GATC 9 cut(s) 114, 162, 338, 966, 993, 1122, 1264, 1493, 1616
MboI GATC 9 cut(s) 112, 160, 336, 964, 991, 1120, 1262, 1491, 1614
MboII GAAGA 8 cut(s) 113, 838, 1114, 1346, 1406, 1460, 1481, 1501
MflI RGATCY 4 cut(s) 112, 964, 1262, 1491
MhlI GDGCHC 1 cut(s) 814
MlyI GAGTC 4 cut(s) 22, 349, 618, 1550
MmeI TCCRAC 1 cut(s) 1421
MseI TTAA 8 cut(s) 123, 222, 861, 867, 909, 924, 960, 1085
MslI CAYNNNNRTG 4 cut(s) 464, 568, 587, 911
MspI CCGG 2 cut(s) 1302, 1344
MspR9I CCNGG 2 cut(s) 660, 680
Mva1269I GAATGC 1 cut(s) 1041
MvaI CCWGG 2 cut(s) 660, 680
MwoI GCNNNNNNNGC 2 cut(s) 556, 1353
NcoI CCATGG 3 cut(s) 182, 1189, 1266
NdeII GATC 9 cut(s) 112, 160, 336, 964, 991, 1120, 1262, 1491, 1614
NheI GCTAGC 1 cut(s) 704
NlaIV GGNNCC 6 cut(s) 114, 558, 722, 920, 1253, 1264
NmuCI GTSAC 1 cut(s) 1238
NspI RCATGY 2 cut(s) 614, 797
PagI TCATGA 1 cut(s) 397
PceI AGGCCT 1 cut(s) 550
PciI ACATGT 2 cut(s) 610, 793
PctI GAATGC 1 cut(s) 1041
PfeI GAWTC 2 cut(s) 175, 1351
PflMI CCANNNNNTGG 2 cut(s) 575, 730
PfoI TCCNGGA 1 cut(s) 678
PkrI GCNGC 2 cut(s) 529, 1300
Ple19I CGATCG 1 cut(s) 339
PleI GAGTC 4 cut(s) 21, 349, 618, 1549
PpsI GAGTC 4 cut(s) 21, 349, 618, 1549
PscI ACATGT 2 cut(s) 610, 793
PshAI GACNNNNGTC 1 cut(s) 1540
PsiI TTATAA 1 cut(s) 689
Psp6I CCWGG 2 cut(s) 658, 678
PspGI CCWGG 2 cut(s) 658, 678
PspN4I GGNNCC 6 cut(s) 114, 558, 722, 920, 1253, 1264
PspPI GGNCC 2 cut(s) 720, 1205
PstI CTGCAG 1 cut(s) 1202
PsuI RGATCY 4 cut(s) 112, 964, 1262, 1491
PvuI CGATCG 1 cut(s) 339
RsaI GTAC 5 cut(s) 66, 311, 668, 1553, 1607
RsaNI GTAC 5 cut(s) 65, 310, 667, 1552, 1606
RseI CAYNNNNRTG 4 cut(s) 464, 568, 587, 911
SalI GTCGAC 1 cut(s) 1533
SaqAI TTAA 8 cut(s) 123, 222, 861, 867, 909, 924, 960, 1085
SatI GCNGC 2 cut(s) 528, 1299
Sau3AI GATC 9 cut(s) 112, 160, 336, 964, 991, 1120, 1262, 1491, 1614
Sau96I GGNCC 2 cut(s) 720, 1205
SchI GAGTC 4 cut(s) 22, 349, 618, 1550
ScrFI CCNGG 2 cut(s) 660, 680
SduI GDGCHC 1 cut(s) 814
SetI ASST 8 cut(s) 255, 804, 924, 1059, 1152, 1210, 1333, 1388
SfaNI GCATC 2 cut(s) 1404, 1462
SfcI CTRYAG 1 cut(s) 1198
SinI GGWCC 1 cut(s) 1205
SmiMI CAYNNNNRTG 4 cut(s) 464, 568, 587, 911
SseBI AGGCCT 1 cut(s) 550
SsiI CCGC 1 cut(s) 270
SspI AATATT 1 cut(s) 522
SspMI CTAG 2 cut(s) 705, 1292
StuI AGGCCT 1 cut(s) 550
StyD4I CCNGG 2 cut(s) 658, 678
StyI CCWWGG 5 cut(s) 182, 533, 1189, 1266, 1324
TaaI ACNGT 5 cut(s) 466, 480, 504, 958, 1321
TaqI TCGA 4 cut(s) 339, 345, 490, 1534
TatI WGTACW 3 cut(s) 64, 309, 1605
TfiI GAWTC 2 cut(s) 175, 1351
Tru1I TTAA 8 cut(s) 123, 222, 861, 867, 909, 924, 960, 1085
Tru9I TTAA 8 cut(s) 123, 222, 861, 867, 909, 924, 960, 1085
TscAI CASTG 7 cut(s) 412, 471, 1056, 1207, 1436, 1485, 1658
TseFI GTSAC 1 cut(s) 1238
TseI GCWGC 2 cut(s) 527, 1298
Tsp45I GTSAC 1 cut(s) 1238
TspDTI ATGAA 6 cut(s) 67, 219, 386, 414, 664, 1461
TspRI CASTG 7 cut(s) 412, 471, 1056, 1207, 1436, 1485, 1658
Van91I CCANNNNNTGG 2 cut(s) 575, 730
VpaK11BI GGWCC 1 cut(s) 1205
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 7 cut(s) 6, 48, 341, 630, 683, 1451, 1657
XceI RCATGY 2 cut(s) 614, 797
XmiI GTMKAC 2 cut(s) 1534, 1581
XspI CTAG 2 cut(s) 705, 1292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.