FvH4_1g17710

U4 U6 small nuclear ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001559
Physical Location & Seq
Forward (+)
10306599 .. 10311449
4851 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g17710.t5

Sequence Viewer

Length: 1461 bp
ATGGCTACTCTTGCCGATTCGTTTCTTGCTGACCTCGATGAGCTGTCTGACAATGAAGCTGATGTTCCTGTTGAAGATGATGGTGATGTTGCGAACATGGAGGAAGATGTTGATGGGGACTTGGCAGACTTGGAGACACTTAACTATGATGATCTGGATAGTGTTTCGAAATTGCAGAAATCGCAAAGATTTGCTGATATAATGCAGAAAGTGGAAGGTGCGCTTGAAAAAGGTTCCGATATGTCAAATCATGCACTAGTTTTGGAAGATGATCCAGAATACCAGTTGATTGTGGACTGTAATGCTCTGTCAGTTGACATTGAGAATGAACTTGTAATCATCCACAATTTTATTCGGGACAAATACCGGCCAAAATTTCCAGAGCTTGAGTCACTTGTTCATCATCCAATTGATTATGCACGAGTTGTTCAGAAAATTGGGAATGAAATGGATGTAACCCTGGTTGATCTGGAGGGGCTTTTACCTTCAGCAACTATTATGGTTGTGTCAGTTACAGCTTCAACTACAAGTGGAAAGCCACTTCCTGAGGATGTCCTGGAGAAAATAGTTGATGCATGTGATCGAGCCCTTGCGCTTGATTCAGCAAAGAAAAAGGTTCTTGAATTTGTTGAAAGTAGAATGGGTTTTATTGCACCAAATCTTTCTGCTATTGTTGGGAGTGCTGTTGCGGCGAAACTTATGGGGATGGCTGGTGGTCTTGCTGCCTTAGCTAAAATGCCTGCTTGTAATGTTCAGCTTCTTGGTGCTAAGAAAAAGAACCTTGCTGGGTTTTCCACTGCAACATCACAATATCGTGTTGGTTATGTTGAGGAGACTGAGATATTTCAAACTACACCCCCTGCTCTTAGGATGCGTGCTGGTAGGCTCTTGGCTGCAAAATCAACCCTTGCAGCACGGGTGGATTCTACAAGAGGAGATCCATCTGGGAACACCGGGAAGGCCTTCCGAGAGGAGATCCATAAGAAAATTGAGAAATGGCAGGAGCCTCCCCCTGCAAAGCAACCTAAACCACTTCCGGTTCCTGATTCTGAACCTAAAAAGAAGAGAGGTGGACGTCGACTGAGGAAAATGAAAGAAAGATATGCGATAACAGACATGAGGAAGCTCGCAAACAGGATGCAGTTTGGCATACCTGAAGAGAGTTCTTTAGGGGATGGACTGGGTGAAGGATATGGAATGCTTGGTCAGGCCGGGAGTGGCAAGTTACGCGTGTCAATGGGTCAGAGCAAACTTGCTGCCAAAGTTGCTAAAAAGTTCAAGGATAAGAATTATGGAAGCGCTGGTGGTGCTACATCTGGACTGACTTCAAGTTTGGCATTTACTCCTGTGCAGGGGATTGAGCTCTCGAATCCTCAGGCTCATGGACAGCAACTTGGCAGTGGAACTCAGAGCACCTACTTCTCTGAAACAGGAACATTTTCAAAAATCAAGAGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Nop Prp31_C

Protein Analysis

487

Amino Acids

52.55

Weight (kDa)

5.54

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop PF01798 132 - 360 3.9e-73 snoRNA binding domain, fibrillarin
Prp31_C PF09785 367 - 486 4.1e-43 Prp31 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1078
AccI GTMKAC 1 cut(s) 1078
AccII CGCG 1 cut(s) 1230
AciI CCGC 1 cut(s) 689
AclWI GGATC 3 cut(s) 266, 932, 970
AcoI YGGCCR 1 cut(s) 368
AcsI RAATTY 2 cut(s) 374, 623
AcuI CTGAAG 2 cut(s) 471, 1176
AcyI GRCGYC 1 cut(s) 1075
AfeI AGCGCT 1 cut(s) 1300
AfiI CCNNNNNNNGG 1 cut(s) 1352
AflIII ACRYGT 1 cut(s) 1228
AgsI TTSAA 9 cut(s) 74, 227, 522, 623, 632, 848, 1279, 1329, 1443
AhlI ACTAGT 1 cut(s) 256
AjnI CCWGG 2 cut(s) 459, 555
AluBI AGCT 8 cut(s) 43, 59, 385, 518, 731, 757, 1126, 1363
AluI AGCT 8 cut(s) 43, 59, 385, 518, 731, 757, 1126, 1363
Alw21I GWGCWC 2 cut(s) 1365, 1415
Alw26I GTCTC 2 cut(s) 128, 827
AlwI GGATC 3 cut(s) 266, 932, 970
Aor51HI AGCGCT 1 cut(s) 1300
AoxI GGCC 3 cut(s) 368, 960, 1209
ApeKI GCWGC 4 cut(s) 722, 893, 911, 1256
ApoI RAATTY 2 cut(s) 374, 623
ArsI GACNNNNNNTTYG 2 cut(s) 1315, 1347
Asp700I GAANNNNTTC 2 cut(s) 962, 1438
AspLEI GCGC 3 cut(s) 223, 595, 1301
AsuC2I CCSGG 2 cut(s) 955, 1213
AsuHPI GGTGA 2 cut(s) 95, 1196
AsuII TTCGAA 1 cut(s) 167
AxyI CCTNAGG 2 cut(s) 546, 1374
BanII GRGCYC 2 cut(s) 589, 1365
BauI CACGAG 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 1365, 1415
BbvI GCAGC 4 cut(s) 709, 880, 923, 1243
BccI CCATC 5 cut(s) 74, 107, 700, 949, 1169
BciT130I CCWGG 2 cut(s) 461, 557
BcnI CCSGG 2 cut(s) 955, 1213
BcoDI GTCTC 2 cut(s) 128, 827
BcuI ACTAGT 1 cut(s) 256
BfaI CTAG 1 cut(s) 257
BfoI RGCGCY 1 cut(s) 1302
BisI GCNGC 5 cut(s) 690, 723, 894, 912, 1257
BlsI GCNGC 5 cut(s) 691, 724, 895, 913, 1258
Bme1390I CCNGG 4 cut(s) 461, 557, 955, 1213
BmiI GGNNCC 3 cut(s) 235, 1005, 1041
BmrFI CCNGG 4 cut(s) 461, 557, 955, 1213
BmrI ACTGGG 1 cut(s) 1190
BmsI GCATC 3 cut(s) 562, 861, 1128
BmuI ACTGGG 1 cut(s) 1190
BpmI CTGGAG 2 cut(s) 491, 578
Bpu10I CCTNAGC 1 cut(s) 727
Bpu14I TTCGAA 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 2 cut(s) 955, 1213
BsaHI GRCGYC 1 cut(s) 1075
BsaJI CCNNGG 1 cut(s) 459
BsaWI WCCGGW 1 cut(s) 1036
Bsc4I CCNNNNNNNGG 1 cut(s) 1352
Bse118I RCCGGY 1 cut(s) 366
Bse1I ACTGG 2 cut(s) 283, 1185
Bse21I CCTNAGG 2 cut(s) 546, 1374
BseBI CCWGG 2 cut(s) 461, 557
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 8 cut(s) 339, 403, 457, 556, 711, 876, 1143, 1180
BseLI CCNNNNNNNGG 1 cut(s) 1352
BseMII CTCAG 5 cut(s) 537, 828, 1073, 1388, 1421
BseNI ACTGG 2 cut(s) 283, 1185
BseRI GAGGAG 3 cut(s) 845, 948, 986
BseXI GCAGC 4 cut(s) 709, 880, 923, 1243
BseYI CCCAGC 1 cut(s) 785
BsgI GTGCAG 1 cut(s) 1370
Bsh1236I CGCG 1 cut(s) 1230
BshFI GGCC 3 cut(s) 370, 962, 1211
BsiHKAI GWGCWC 2 cut(s) 1365, 1415
BsiSI CCGG 4 cut(s) 367, 954, 1037, 1212
BslFI GGGAC 2 cut(s) 131, 371
BslI CCNNNNNNNGG 1 cut(s) 1352
BsmAI GTCTC 2 cut(s) 128, 827
BsmFI GGGAC 2 cut(s) 131, 371
BsmI GAATGC 1 cut(s) 1203
BsnI GGCC 3 cut(s) 370, 962, 1211
Bsp119I TTCGAA 1 cut(s) 167
Bsp1286I GDGCHC 3 cut(s) 589, 1365, 1415
Bsp143I GATC 7 cut(s) 151, 271, 466, 580, 937, 975, 1455
BspACI CCGC 1 cut(s) 689
BspANI GGCC 3 cut(s) 370, 962, 1211
BspCNI CTCAG 5 cut(s) 538, 829, 1074, 1387, 1420
BspFNI CGCG 1 cut(s) 1230
BspLI GGNNCC 3 cut(s) 235, 1005, 1041
BspPI GGATC 3 cut(s) 266, 932, 970
BspT104I TTCGAA 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 366
BsrI ACTGG 2 cut(s) 283, 1185
BssAI RCCGGY 1 cut(s) 366
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 7 cut(s) 151, 271, 466, 580, 937, 975, 1455
BssNI GRCGYC 1 cut(s) 1075
BssSI CACGAG 1 cut(s) 420
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 2 cut(s) 461, 557
Bst4CI ACNGT 1 cut(s) 299
Bst6I CTCTTC 2 cut(s) 1058, 1152
BstACI GRCGYC 1 cut(s) 1075
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 3 cut(s) 741, 876, 1128
BstDEI CTNAG 8 cut(s) 546, 727, 768, 837, 866, 1082, 1374, 1407
BstF5I GGATG 8 cut(s) 339, 403, 457, 556, 711, 876, 1143, 1180
BstFNI CGCG 1 cut(s) 1230
BstH2I RGCGCY 1 cut(s) 1302
BstHHI GCGC 3 cut(s) 223, 595, 1301
BstKTI GATC 7 cut(s) 154, 274, 469, 583, 940, 978, 1458
BstMAI GTCTC 2 cut(s) 128, 827
BstMBI GATC 7 cut(s) 151, 271, 466, 580, 937, 975, 1455
BstMWI GCNNNNNNNGC 7 cut(s) 11, 181, 689, 728, 1227, 1265, 1307
BstNI CCWGG 2 cut(s) 461, 557
BstNSI RCATGY 1 cut(s) 579
BstSCI CCNGG 4 cut(s) 459, 555, 953, 1211
BstUI CGCG 1 cut(s) 1230
BstV1I GCAGC 4 cut(s) 709, 880, 923, 1243
BstX2I RGATCY 3 cut(s) 937, 975, 1455
BstYI RGATCY 3 cut(s) 937, 975, 1455
Bsu36I CCTNAGG 2 cut(s) 546, 1374
BsuRI GGCC 3 cut(s) 370, 962, 1211
BtsCI GGATG 8 cut(s) 339, 403, 457, 556, 711, 876, 1143, 1180
BtsI GCAGTG 2 cut(s) 795, 1405
BtsIMutI CAGTG 2 cut(s) 795, 1405
Cac8I GCNNGC 3 cut(s) 741, 876, 1128
CfoI GCGC 3 cut(s) 223, 595, 1301
Cfr10I RCCGGY 1 cut(s) 366
CviAII CATG 5 cut(s) 97, 251, 576, 1117, 1382
DdeI CTNAG 8 cut(s) 546, 727, 768, 837, 866, 1082, 1374, 1407
DpnI GATC 7 cut(s) 153, 273, 468, 582, 939, 977, 1457
DpnII GATC 7 cut(s) 151, 271, 466, 580, 937, 975, 1455
EaeI YGGCCR 1 cut(s) 368
Eam1104I CTCTTC 2 cut(s) 1058, 1152
EarI CTCTTC 2 cut(s) 1058, 1152
Ecl136II GAGCTC 1 cut(s) 1363
Eco147I AGGCCT 1 cut(s) 962
Eco24I GRGCYC 2 cut(s) 589, 1365
Eco47III AGCGCT 1 cut(s) 1300
Eco53kI GAGCTC 1 cut(s) 1363
Eco57I CTGAAG 2 cut(s) 471, 1176
Eco81I CCTNAGG 2 cut(s) 546, 1374
EcoICRI GAGCTC 1 cut(s) 1363
EcoRII CCWGG 2 cut(s) 459, 555
EcoT22I ATGCAT 1 cut(s) 577
EcoT38I GRGCYC 2 cut(s) 589, 1365
FaeI CATG 5 cut(s) 100, 254, 579, 1120, 1385
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FaqI GGGAC 2 cut(s) 131, 371
FatI CATG 5 cut(s) 96, 250, 575, 1116, 1381
FblI GTMKAC 1 cut(s) 1078
Fnu4HI GCNGC 5 cut(s) 690, 723, 894, 912, 1257
FokI GGATG 8 cut(s) 326, 390, 464, 563, 718, 883, 1150, 1187
FriOI GRGCYC 2 cut(s) 589, 1365
Fsp4HI GCNGC 5 cut(s) 690, 723, 894, 912, 1257
FspBI CTAG 1 cut(s) 257
GlaI GCGC 3 cut(s) 222, 594, 1300
GluI GCNGC 5 cut(s) 690, 723, 894, 912, 1257
GsaI CCCAGC 1 cut(s) 789
GsuI CTGGAG 2 cut(s) 491, 578
HaeII RGCGCY 1 cut(s) 1302
HaeIII GGCC 3 cut(s) 370, 962, 1211
HapII CCGG 4 cut(s) 367, 954, 1037, 1212
HhaI GCGC 3 cut(s) 223, 595, 1301
Hin1I GRCGYC 1 cut(s) 1075
Hin1II CATG 5 cut(s) 100, 254, 579, 1120, 1385
Hin6I GCGC 3 cut(s) 221, 593, 1299
HinP1I GCGC 3 cut(s) 221, 593, 1299
HincII GTYRAC 2 cut(s) 316, 1079
HindII GTYRAC 2 cut(s) 316, 1079
HinfI GANTC 6 cut(s) 17, 389, 599, 923, 1046, 1369
HpaII CCGG 4 cut(s) 367, 954, 1037, 1212
HphI GGTGA 2 cut(s) 95, 1196
Hpy166II GTNNAC 4 cut(s) 295, 316, 1073, 1079
Hpy188I TCNGA 9 cut(s) 49, 238, 432, 968, 1051, 1245, 1410, 1426, 1460
Hpy8I GTNNAC 4 cut(s) 295, 316, 1073, 1079
Hpy99I CGWCG 1 cut(s) 1080
HpyAV CCTTC 5 cut(s) 209, 495, 952, 973, 1181
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4IV ACGT 1 cut(s) 1075
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 181, 689, 728, 1227, 1265, 1307
HpyF3I CTNAG 8 cut(s) 546, 727, 768, 837, 866, 1082, 1374, 1407
HpySE526I ACGT 1 cut(s) 1075
Hsp92I GRCGYC 1 cut(s) 1075
Hsp92II CATG 5 cut(s) 100, 254, 579, 1120, 1385
HspAI GCGC 3 cut(s) 221, 593, 1299
Kzo9I GATC 7 cut(s) 151, 271, 466, 580, 937, 975, 1455
LmnI GCTCC 1 cut(s) 1003
Lsp1109I GCAGC 4 cut(s) 709, 880, 923, 1243
LweI GCATC 3 cut(s) 562, 861, 1128
MaeI CTAG 1 cut(s) 257
MaeII ACGT 1 cut(s) 1075
MaeIII GTNAC 4 cut(s) 390, 454, 511, 1224
MalI GATC 7 cut(s) 153, 273, 468, 582, 939, 977, 1457
MboI GATC 7 cut(s) 151, 271, 466, 580, 937, 975, 1455
MboII GAAGA 5 cut(s) 86, 116, 278, 1075, 1169
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 3 cut(s) 937, 975, 1455
MhlI GDGCHC 3 cut(s) 589, 1365, 1415
MluCI AATT 8 cut(s) 170, 346, 374, 408, 435, 623, 987, 1288
MluI ACGCGT 1 cut(s) 1228
MlyI GAGTC 1 cut(s) 398
Mph1103I ATGCAT 1 cut(s) 577
MroXI GAANNNNTTC 2 cut(s) 962, 1438
MseI TTAA 1 cut(s) 141
MspI CCGG 4 cut(s) 367, 954, 1037, 1212
MspR9I CCNGG 4 cut(s) 461, 557, 955, 1213
MunI CAATTG 1 cut(s) 408
Mva1269I GAATGC 1 cut(s) 1203
MvaI CCWGG 2 cut(s) 461, 557
MvnI CGCG 1 cut(s) 1230
MwoI GCNNNNNNNGC 7 cut(s) 11, 181, 689, 728, 1227, 1265, 1307
NciI CCSGG 2 cut(s) 955, 1213
NdeII GATC 7 cut(s) 151, 271, 466, 580, 937, 975, 1455
NlaIII CATG 5 cut(s) 100, 254, 579, 1120, 1385
NlaIV GGNNCC 3 cut(s) 235, 1005, 1041
NmuCI GTSAC 1 cut(s) 390
NsiI ATGCAT 1 cut(s) 577
NspI RCATGY 1 cut(s) 579
NspV TTCGAA 1 cut(s) 167
PceI AGGCCT 1 cut(s) 962
PctI GAATGC 1 cut(s) 1203
PdmI GAANNNNTTC 2 cut(s) 962, 1438
PfeI GAWTC 5 cut(s) 17, 599, 923, 1046, 1369
PfoI TCCNGGA 1 cut(s) 555
PkrI GCNGC 5 cut(s) 691, 724, 895, 913, 1258
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
Psp124BI GAGCTC 1 cut(s) 1365
Psp6I CCWGG 2 cut(s) 459, 555
PspFI CCCAGC 1 cut(s) 785
PspGI CCWGG 2 cut(s) 459, 555
PspN4I GGNNCC 3 cut(s) 235, 1005, 1041
PsuI RGATCY 3 cut(s) 937, 975, 1455
SacI GAGCTC 1 cut(s) 1365
SalI GTCGAC 1 cut(s) 1077
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 5 cut(s) 690, 723, 894, 912, 1257
Sau3AI GATC 7 cut(s) 151, 271, 466, 580, 937, 975, 1455
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 4 cut(s) 461, 557, 955, 1213
SduI GDGCHC 3 cut(s) 589, 1365, 1415
SfaNI GCATC 3 cut(s) 562, 861, 1128
SfuI TTCGAA 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
SpeI ACTAGT 1 cut(s) 256
Sse9I AATT 8 cut(s) 170, 346, 374, 408, 435, 623, 987, 1288
SseBI AGGCCT 1 cut(s) 962
SsiI CCGC 1 cut(s) 689
SspMI CTAG 1 cut(s) 257
SstI GAGCTC 1 cut(s) 1365
StuI AGGCCT 1 cut(s) 962
StyD4I CCNGG 4 cut(s) 459, 555, 953, 1211
TaaI ACNGT 1 cut(s) 299
TaiI ACGT 1 cut(s) 1078
TaqI TCGA 5 cut(s) 36, 167, 583, 1078, 1367
TasI AATT 8 cut(s) 170, 346, 374, 408, 435, 623, 987, 1288
TauI GCSGC 1 cut(s) 692
TfiI GAWTC 5 cut(s) 17, 599, 923, 1046, 1369
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 2 cut(s) 802, 1405
TseFI GTSAC 1 cut(s) 390
TseI GCWGC 4 cut(s) 722, 893, 911, 1256
Tsp45I GTSAC 1 cut(s) 390
TspDTI ATGAA 5 cut(s) 69, 342, 389, 459, 1106
TspRI CASTG 2 cut(s) 802, 1405
XapI RAATTY 2 cut(s) 374, 623
XceI RCATGY 1 cut(s) 579
XmiI GTMKAC 1 cut(s) 1078
XmnI GAANNNNTTC 2 cut(s) 962, 1438
XspI CTAG 1 cut(s) 257
ZraI GACGTC 1 cut(s) 1076
Zsp2I ATGCAT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.