Rw2G015790

U4 U6 small nuclear ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001559
Physical Location & Seq
Forward (+)
18433725 .. 18437260
3536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G015790.1

Sequence Viewer

Length: 1536 bp
ATGATCCAAGCCCACCTCAGCCCAACCCGCCCTTCTGCCGGCACTACTCGCCGGAAGCTTCTTGAGCCTGAAACAATGGCAACTCTAGCCGATTCATTTCTTGCTGATCTTGATGAGCTATCGGACAATGAAGCCGATGTTCCTGTTGAAGATGATGCTGATGTTGCAAACATGGAGGAAGATGTCGATGGGGACTTGGCGGACTTAGAGACACTTAACTATGATGATCTGGATAGTGTTTCAAAATTGCAGAAATCACAAAGATTTGCTGATATAATGCAGAAAGTGGAAGGTGCACTTGAGAAGGGTTCTGATATGTCAAACCATGCACTAGTTTTGGAAGATGATCCAGAATATCAGCTGATTGTGGACTGTAATGCTTTGTCAGTTGACATTGAGAATGAACTTGTAATCATCCACAATTTTATTCGTGACAAATACCGGCCAAAATTTCCAGAGCTCGAGTCACTTGTTCATCATCCAATTGATTATGCTCGAGTTGTTCAGAAAATTGGGAATGAAATGGATGTAACCCTTGTTGATCTGGAGGGGCTTTTACCTTCAGCAACTATTATGGTTGTGTCAGTTACAGCTTCAACTACAAGTGGAAAGCCACTTCCAGAGGACGTCCTGGAGAAAATAGTTGATGCATGTGATCGAGCCCTTGCTCTTGATTCAGCAAAGAAAAAAGTTCTTGATTTTGTAGAAAGTAGAATGGGTTTTATTGCACCAAATCTTTCTGCTATTGTTGGGAGTGCTGTTGCGGCGAAACTTATGGGGATGGCTGGTGGTCTTGCTGCCTTAGCTAAAATGCCTGCTTGTAATGTTCAGCTTCTTGGTGCTAAGAAAAAAAATCTTGCTGGGTTTTCCACTGCAACATCACAATATCGTATTGGTTATGTTGAGGAGACAGAGATATTTCAAACTACACCCCCTGCTCTTAGGATGCGTGCTGGTCGGCTCTTGGCTGCAAAATCAACCCTTGCAGCGCGGGTGGATTCTACGAGAGGAGATCCATCTGGGAACACTGGAAAGGCATTCCGAGAGGAGATCCGTAAGAAAATTGAAAAATGGCAGGAGCCTCCCCCTGCAAAGCAACCTAAACCACTTCCCGTTCCTGATTCTGAACCTAAAAAGAAGAGAGGTGGACGTCGACTCAGGAAGATGAAAGAAAGATATGCGATAACAGACATGAGGAAGCTCGCAAACAGGATGCAGTTTGGCATACCTGAAGAGAGTTCTTTAGGGGATGGACTGGGTGAAGGATATGGAATGCTTGGTCAGGCTGGGAGTGGCAAGCTGCGTGTGTCGATGGGTCAGAGCAAACTTGCTGCTAAAGTTGCTAAAAAGTTTAAGGATAAGAATTATGGAAGCACTGGTGGTGCTACATCTGGACTGACTTCAAGTTTGGCATTTACTCCTGTGCAGGGGATTGAGCTCTCAAATCCTCAGGCCCATGGACAGCAGCTTGGCAGTGGAACTCAAAGCACCTATTTCTCTGAAACAGGAACATTTTCAAAAATCAAGAGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Nop Prp31_C

Protein Analysis

511

Amino Acids

55.39

Weight (kDa)

5.89

Isoelectric Point (pI)

38.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop PF01798 129 - 357 7.7e-73 snoRNA binding domain, fibrillarin
Prp31_C PF09785 364 - 483 2.8e-43 Prp31 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 630, 1153
AccI GTMKAC 1 cut(s) 1153
AccII CGCG 1 cut(s) 991
AciI CCGC 4 cut(s) 28, 200, 764, 991
AclWI GGATC 3 cut(s) 341, 1007, 1045
AcoI YGGCCR 1 cut(s) 443
AcsI RAATTY 1 cut(s) 449
AcuI CTGAAG 2 cut(s) 546, 1251
AcyI GRCGYC 2 cut(s) 627, 1150
AfiI CCNNNNNNNGG 2 cut(s) 38, 1427
AgsI TTSAA 7 cut(s) 149, 243, 597, 923, 1067, 1404, 1518
AhlI ACTAGT 1 cut(s) 331
AjnI CCWGG 1 cut(s) 630
AjuI GAANNNNNNNTTGG 2 cut(s) 16, 48
Alw21I GWGCWC 3 cut(s) 298, 462, 1440
Alw26I GTCTC 2 cut(s) 203, 902
Alw44I GTGCAC 1 cut(s) 294
AlwI GGATC 3 cut(s) 341, 1007, 1045
Ama87I CYCGRG 2 cut(s) 461, 495
AoxI GGCC 2 cut(s) 443, 1452
ApaLI GTGCAC 1 cut(s) 294
ApeKI GCWGC 6 cut(s) 797, 968, 986, 1300, 1331, 1465
ApoI RAATTY 1 cut(s) 449
ArsI GACNNNNNNTTYG 2 cut(s) 1390, 1422
Asp700I GAANNNNTTC 1 cut(s) 1513
AspLEI GCGC 1 cut(s) 991
AspS9I GGNCC 1 cut(s) 1453
AsuHPI GGTGA 1 cut(s) 1271
AvaI CYCGRG 2 cut(s) 461, 495
AxyI CCTNAGG 1 cut(s) 1449
BaeGI GKGCMC 1 cut(s) 298
BanII GRGCYC 3 cut(s) 462, 664, 1440
Bbv12I GWGCWC 3 cut(s) 298, 462, 1440
BbvCI CCTCAGC 1 cut(s) 17
BbvI GCAGC 6 cut(s) 784, 955, 998, 1287, 1318, 1477
BccI CCATC 5 cut(s) 182, 775, 1024, 1244, 1306
BciT130I CCWGG 1 cut(s) 632
BcoDI GTCTC 2 cut(s) 203, 902
BcuI ACTAGT 1 cut(s) 331
BfaI CTAG 2 cut(s) 86, 332
BisI GCNGC 7 cut(s) 765, 798, 969, 987, 1301, 1332, 1466
BlsI GCNGC 7 cut(s) 766, 799, 970, 988, 1302, 1333, 1467
Bme1390I CCNGG 1 cut(s) 632
BmeT110I CYCGRG 2 cut(s) 461, 495
BmgT120I GGNCC 1 cut(s) 1453
BmiI GGNNCC 1 cut(s) 1080
BmrFI CCNGG 1 cut(s) 632
BmrI ACTGGG 1 cut(s) 1265
BmsI GCATC 4 cut(s) 145, 637, 936, 1203
BmuI ACTGGG 1 cut(s) 1265
BpmI CTGGAG 2 cut(s) 566, 653
Bpu10I CCTNAGC 2 cut(s) 17, 802
BpuEI CTTGAG 2 cut(s) 83, 320
BsaHI GRCGYC 2 cut(s) 627, 1150
BsaJI CCNNGG 1 cut(s) 1456
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 1427
Bse118I RCCGGY 2 cut(s) 38, 441
Bse1I ACTGG 3 cut(s) 1033, 1260, 1381
Bse21I CCTNAGG 1 cut(s) 1449
BseBI CCWGG 1 cut(s) 632
BseDI CCNNGG 1 cut(s) 1456
BseGI GGATG 7 cut(s) 414, 478, 532, 786, 951, 1218, 1255
BseLI CCNNNNNNNGG 2 cut(s) 38, 1427
BseMII CTCAG 3 cut(s) 31, 1171, 1463
BseNI ACTGG 3 cut(s) 1033, 1260, 1381
BseRI GAGGAG 3 cut(s) 920, 1023, 1061
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 6 cut(s) 784, 955, 998, 1287, 1318, 1477
BseYI CCCAGC 2 cut(s) 860, 1286
BsgI GTGCAG 1 cut(s) 1445
Bsh1236I CGCG 1 cut(s) 991
BshFI GGCC 2 cut(s) 445, 1454
BsiHKAI GWGCWC 3 cut(s) 298, 462, 1440
BsiHKCI CYCGRG 2 cut(s) 461, 495
BsiSI CCGG 3 cut(s) 39, 52, 442
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 38, 1427
BsmAI GTCTC 2 cut(s) 203, 902
BsmFI GGGAC 1 cut(s) 206
BsmI GAATGC 2 cut(s) 1037, 1278
BsnI GGCC 2 cut(s) 445, 1454
BsoBI CYCGRG 2 cut(s) 461, 495
Bsp1286I GDGCHC 4 cut(s) 298, 462, 664, 1440
Bsp143I GATC 9 cut(s) 3, 106, 226, 346, 541, 655, 1012, 1050, 1530
Bsp19I CCATGG 1 cut(s) 1456
BspACI CCGC 4 cut(s) 28, 200, 764, 991
BspANI GGCC 2 cut(s) 445, 1454
BspCNI CTCAG 3 cut(s) 30, 1170, 1462
BspFNI CGCG 1 cut(s) 991
BspLI GGNNCC 1 cut(s) 1080
BspPI GGATC 3 cut(s) 341, 1007, 1045
BsrFI RCCGGY 2 cut(s) 38, 441
BsrI ACTGG 3 cut(s) 1033, 1260, 1381
BssAI RCCGGY 2 cut(s) 38, 441
BssECI CCNNGG 1 cut(s) 1456
BssMI GATC 9 cut(s) 3, 106, 226, 346, 541, 655, 1012, 1050, 1530
BssNI GRCGYC 2 cut(s) 627, 1150
BssT1I CCWWGG 1 cut(s) 1456
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 1 cut(s) 374
Bst6I CTCTTC 2 cut(s) 1133, 1227
BstACI GRCGYC 2 cut(s) 627, 1150
BstC8I GCNNGC 5 cut(s) 40, 816, 951, 1203, 1298
BstDEI CTNAG 7 cut(s) 17, 205, 802, 843, 941, 1157, 1449
BstDSI CCRYGG 1 cut(s) 1456
BstF5I GGATG 7 cut(s) 414, 478, 532, 786, 951, 1218, 1255
BstFNI CGCG 1 cut(s) 991
BstHHI GCGC 1 cut(s) 991
BstKTI GATC 9 cut(s) 6, 109, 229, 349, 544, 658, 1015, 1053, 1533
BstMAI GTCTC 2 cut(s) 203, 902
BstMBI GATC 9 cut(s) 3, 106, 226, 346, 541, 655, 1012, 1050, 1530
BstMWI GCNNNNNNNGC 8 cut(s) 27, 48, 64, 86, 164, 764, 803, 1340
BstNI CCWGG 1 cut(s) 632
BstNSI RCATGY 1 cut(s) 654
BstSCI CCNGG 1 cut(s) 630
BstSLI GKGCMC 1 cut(s) 298
BstUI CGCG 1 cut(s) 991
BstV1I GCAGC 6 cut(s) 784, 955, 998, 1287, 1318, 1477
BstX2I RGATCY 3 cut(s) 1012, 1050, 1530
BstYI RGATCY 3 cut(s) 1012, 1050, 1530
Bsu36I CCTNAGG 1 cut(s) 1449
BsuRI GGCC 2 cut(s) 445, 1454
BtgI CCRYGG 1 cut(s) 1456
BtsCI GGATG 7 cut(s) 414, 478, 532, 786, 951, 1218, 1255
BtsI GCAGTG 2 cut(s) 870, 1480
BtsIMutI CAGTG 4 cut(s) 870, 1026, 1374, 1480
Cac8I GCNNGC 5 cut(s) 40, 816, 951, 1203, 1298
CfoI GCGC 1 cut(s) 991
Cfr10I RCCGGY 2 cut(s) 38, 441
Cfr13I GGNCC 1 cut(s) 1453
CviAII CATG 5 cut(s) 172, 326, 651, 1192, 1457
DdeI CTNAG 7 cut(s) 17, 205, 802, 843, 941, 1157, 1449
DpnI GATC 9 cut(s) 5, 108, 228, 348, 543, 657, 1014, 1052, 1532
DpnII GATC 9 cut(s) 3, 106, 226, 346, 541, 655, 1012, 1050, 1530
EaeI YGGCCR 1 cut(s) 443
Eam1104I CTCTTC 2 cut(s) 1133, 1227
EarI CTCTTC 2 cut(s) 1133, 1227
EciI GGCGGA 1 cut(s) 215
Ecl136II GAGCTC 2 cut(s) 460, 1438
Eco130I CCWWGG 1 cut(s) 1456
Eco24I GRGCYC 3 cut(s) 462, 664, 1440
Eco53kI GAGCTC 2 cut(s) 460, 1438
Eco57I CTGAAG 2 cut(s) 546, 1251
Eco81I CCTNAGG 1 cut(s) 1449
Eco88I CYCGRG 2 cut(s) 461, 495
EcoICRI GAGCTC 2 cut(s) 460, 1438
EcoRII CCWGG 1 cut(s) 630
EcoT14I CCWWGG 1 cut(s) 1456
EcoT22I ATGCAT 1 cut(s) 652
EcoT38I GRGCYC 3 cut(s) 462, 664, 1440
ErhI CCWWGG 1 cut(s) 1456
FaeI CATG 5 cut(s) 175, 329, 654, 1195, 1460
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FaqI GGGAC 1 cut(s) 206
FatI CATG 5 cut(s) 171, 325, 650, 1191, 1456
FauI CCCGC 2 cut(s) 35, 984
FblI GTMKAC 1 cut(s) 1153
Fnu4HI GCNGC 7 cut(s) 765, 798, 969, 987, 1301, 1332, 1466
FokI GGATG 7 cut(s) 401, 465, 539, 793, 958, 1225, 1262
FriOI GRGCYC 3 cut(s) 462, 664, 1440
Fsp4HI GCNGC 7 cut(s) 765, 798, 969, 987, 1301, 1332, 1466
FspBI CTAG 2 cut(s) 86, 332
GlaI GCGC 1 cut(s) 990
GluI GCNGC 7 cut(s) 765, 798, 969, 987, 1301, 1332, 1466
GsaI CCCAGC 2 cut(s) 864, 1290
GsuI CTGGAG 2 cut(s) 566, 653
HaeIII GGCC 2 cut(s) 445, 1454
HapII CCGG 3 cut(s) 39, 52, 442
HhaI GCGC 1 cut(s) 991
Hin1I GRCGYC 2 cut(s) 627, 1150
Hin1II CATG 5 cut(s) 175, 329, 654, 1195, 1460
Hin6I GCGC 1 cut(s) 989
HinP1I GCGC 1 cut(s) 989
HincII GTYRAC 2 cut(s) 391, 1154
HindII GTYRAC 2 cut(s) 391, 1154
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 6 cut(s) 92, 464, 674, 998, 1121, 1155
HpaII CCGG 3 cut(s) 39, 52, 442
HphI GGTGA 1 cut(s) 1271
Hpy166II GTNNAC 5 cut(s) 296, 370, 391, 1148, 1154
Hpy188I TCNGA 8 cut(s) 124, 313, 507, 1043, 1126, 1320, 1501, 1535
Hpy8I GTNNAC 5 cut(s) 296, 370, 391, 1148, 1154
Hpy99I CGWCG 1 cut(s) 1155
HpyAV CCTTC 5 cut(s) 42, 284, 298, 570, 1256
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 2 cut(s) 627, 1150
HpyF10VI GCNNNNNNNGC 8 cut(s) 27, 48, 64, 86, 164, 764, 803, 1340
HpyF3I CTNAG 7 cut(s) 17, 205, 802, 843, 941, 1157, 1449
HpySE526I ACGT 2 cut(s) 627, 1150
Hsp92I GRCGYC 2 cut(s) 627, 1150
Hsp92II CATG 5 cut(s) 175, 329, 654, 1195, 1460
HspAI GCGC 1 cut(s) 989
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Kzo9I GATC 9 cut(s) 3, 106, 226, 346, 541, 655, 1012, 1050, 1530
LmnI GCTCC 1 cut(s) 1078
Lsp1109I GCAGC 6 cut(s) 784, 955, 998, 1287, 1318, 1477
LweI GCATC 4 cut(s) 145, 637, 936, 1203
MaeI CTAG 2 cut(s) 86, 332
MaeII ACGT 2 cut(s) 627, 1150
MaeIII GTNAC 4 cut(s) 431, 465, 529, 586
MalI GATC 9 cut(s) 5, 108, 228, 348, 543, 657, 1014, 1052, 1532
MboI GATC 9 cut(s) 3, 106, 226, 346, 541, 655, 1012, 1050, 1530
MboII GAAGA 6 cut(s) 161, 191, 353, 1150, 1174, 1244
MfeI CAATTG 1 cut(s) 483
MflI RGATCY 3 cut(s) 1012, 1050, 1530
MhlI GDGCHC 4 cut(s) 298, 462, 664, 1440
MluCI AATT 7 cut(s) 245, 421, 449, 483, 510, 1062, 1363
MlyI GAGTC 2 cut(s) 473, 1149
Mph1103I ATGCAT 1 cut(s) 652
MroNI GCCGGC 1 cut(s) 38
MroXI GAANNNNTTC 1 cut(s) 1513
MseI TTAA 2 cut(s) 216, 1353
MspA1I CMGCKG 1 cut(s) 361
MspI CCGG 3 cut(s) 39, 52, 442
MspR9I CCNGG 1 cut(s) 632
MunI CAATTG 1 cut(s) 483
Mva1269I GAATGC 2 cut(s) 1037, 1278
MvaI CCWGG 1 cut(s) 632
MvnI CGCG 1 cut(s) 991
MwoI GCNNNNNNNGC 8 cut(s) 27, 48, 64, 86, 164, 764, 803, 1340
NaeI GCCGGC 1 cut(s) 40
NcoI CCATGG 1 cut(s) 1456
NdeII GATC 9 cut(s) 3, 106, 226, 346, 541, 655, 1012, 1050, 1530
NgoMIV GCCGGC 1 cut(s) 38
NlaIII CATG 5 cut(s) 175, 329, 654, 1195, 1460
NlaIV GGNNCC 1 cut(s) 1080
NmuCI GTSAC 2 cut(s) 431, 465
NsiI ATGCAT 1 cut(s) 652
NspI RCATGY 1 cut(s) 654
PaeR7I CTCGAG 2 cut(s) 461, 495
PctI GAATGC 2 cut(s) 1037, 1278
PdiI GCCGGC 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 1513
PfeI GAWTC 4 cut(s) 92, 674, 998, 1121
PfoI TCCNGGA 1 cut(s) 630
PkrI GCNGC 7 cut(s) 766, 799, 970, 988, 1302, 1333, 1467
PleI GAGTC 2 cut(s) 472, 1149
PpsI GAGTC 2 cut(s) 472, 1149
Psp124BI GAGCTC 2 cut(s) 462, 1440
Psp6I CCWGG 1 cut(s) 630
PspFI CCCAGC 2 cut(s) 860, 1286
PspGI CCWGG 1 cut(s) 630
PspN4I GGNNCC 1 cut(s) 1080
PspPI GGNCC 1 cut(s) 1453
PspXI VCTCGAGB 2 cut(s) 461, 495
PsuI RGATCY 3 cut(s) 1012, 1050, 1530
PvuII CAGCTG 1 cut(s) 361
SacI GAGCTC 2 cut(s) 462, 1440
SalI GTCGAC 1 cut(s) 1152
SaqAI TTAA 2 cut(s) 216, 1353
SatI GCNGC 7 cut(s) 765, 798, 969, 987, 1301, 1332, 1466
Sau3AI GATC 9 cut(s) 3, 106, 226, 346, 541, 655, 1012, 1050, 1530
Sau96I GGNCC 1 cut(s) 1453
SchI GAGTC 2 cut(s) 473, 1149
ScrFI CCNGG 1 cut(s) 632
SduI GDGCHC 4 cut(s) 298, 462, 664, 1440
SfaNI GCATC 4 cut(s) 145, 637, 936, 1203
Sfr274I CTCGAG 2 cut(s) 461, 495
SlaI CTCGAG 2 cut(s) 461, 495
SmlI CTYRAG 4 cut(s) 62, 299, 461, 495
SmoI CTYRAG 4 cut(s) 62, 299, 461, 495
SpeI ACTAGT 1 cut(s) 331
Sse9I AATT 7 cut(s) 245, 421, 449, 483, 510, 1062, 1363
SsiI CCGC 4 cut(s) 28, 200, 764, 991
SspMI CTAG 2 cut(s) 86, 332
SstI GAGCTC 2 cut(s) 462, 1440
StyD4I CCNGG 1 cut(s) 630
StyI CCWWGG 1 cut(s) 1456
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 2 cut(s) 630, 1153
TaqI TCGA 6 cut(s) 186, 462, 496, 658, 1153, 1310
TasI AATT 7 cut(s) 245, 421, 449, 483, 510, 1062, 1363
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 4 cut(s) 92, 674, 998, 1121
Tru1I TTAA 2 cut(s) 216, 1353
Tru9I TTAA 2 cut(s) 216, 1353
TscAI CASTG 4 cut(s) 877, 1033, 1381, 1480
TseFI GTSAC 2 cut(s) 431, 465
TseI GCWGC 6 cut(s) 797, 968, 986, 1300, 1331, 1465
Tsp45I GTSAC 2 cut(s) 431, 465
TspDTI ATGAA 6 cut(s) 84, 144, 417, 464, 534, 1181
TspGWI ACGGA 1 cut(s) 1043
TspRI CASTG 4 cut(s) 877, 1033, 1381, 1480
VneI GTGCAC 1 cut(s) 294
XapI RAATTY 1 cut(s) 449
XceI RCATGY 1 cut(s) 654
XhoI CTCGAG 2 cut(s) 461, 495
XmiI GTMKAC 1 cut(s) 1153
XmnI GAANNNNTTC 1 cut(s) 1513
XspI CTAG 2 cut(s) 86, 332
ZraI GACGTC 2 cut(s) 628, 1151
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.