Rroxscaffold_2G00135680

U4 U6 small nuclear ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001559
Physical Location & Seq
Reverse (-)
73260132 .. 73263744
3613 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00135680.1

Sequence Viewer

Length: 591 bp
ATGCGTGCTGGTCGGCTCTTGGCTGCAAAATCAACCCTTGCAGCACGGGTGGATTCTACAAGAGGAGATCCATCTGGGAACACTGGAAAGGCATTCCGAGAGGAGATCCATAAGAAAATTGAAAAATGGCAGGAGCCTCCCCCTGCCAAGCAACCTAAACCACTTCCGGTTCCTGATTCTGAACCTAAAAAGAAGAGAGGTGGACGTCGACTCAGGAAGATGAAAGAAAGATATGCGATAACAGACATGAGGAAGCTCGCAAACAGGATGCAGTTTGGCATACCTGAAGAGAGTTCTTTAGGGGATGGACTGGGTGAAGGATATGGAATGCTTGGTCAGGCTGGGAGTGGCAAGCTGCGTGTGTCGATGGGTCAGAGCAAACTTGCTGCTAAAGTTGCTAAAAAGTTTAAGGATAAGAATTATGGAAGCACTGGTGGTGCTACATCTGGACTGACTTCAAGTTTGGCATTTACTCCTGTGCAGGGGATTGAGCTCTCAAATCCTCAGGCCCATGGACAGCAGCTTGGCAGTGGAACTCAAAGCACCTATTTCTCTGAAACAGGAACATTTTCAAAAATCAAGAGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Nop Prp31_C

Protein Analysis

196

Amino Acids

21.15

Weight (kDa)

10.33

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop PF01798 2 - 42 6.2e-07 snoRNA binding domain, fibrillarin
Prp31_C PF09785 49 - 168 3.4e-44 Prp31 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 208
AccI GTMKAC 1 cut(s) 208
AclWI GGATC 2 cut(s) 62, 100
AcuI CTGAAG 1 cut(s) 306
AcyI GRCGYC 1 cut(s) 205
AfiI CCNNNNNNNGG 1 cut(s) 482
AgsI TTSAA 3 cut(s) 122, 459, 573
AluBI AGCT 4 cut(s) 256, 355, 493, 523
AluI AGCT 4 cut(s) 256, 355, 493, 523
Alw21I GWGCWC 1 cut(s) 495
AlwI GGATC 2 cut(s) 62, 100
AoxI GGCC 1 cut(s) 507
ApeKI GCWGC 5 cut(s) 23, 41, 355, 386, 520
ArsI GACNNNNNNTTYG 2 cut(s) 445, 477
Asp700I GAANNNNTTC 1 cut(s) 568
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 1 cut(s) 326
AxyI CCTNAGG 1 cut(s) 504
BanII GRGCYC 1 cut(s) 495
Bbv12I GWGCWC 1 cut(s) 495
BbvI GCAGC 5 cut(s) 10, 53, 342, 373, 532
BccI CCATC 3 cut(s) 79, 299, 361
BisI GCNGC 5 cut(s) 24, 42, 356, 387, 521
BlsI GCNGC 5 cut(s) 25, 43, 357, 388, 522
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 2 cut(s) 135, 171
BmrI ACTGGG 1 cut(s) 320
BmsI GCATC 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 320
BsaHI GRCGYC 1 cut(s) 205
BsaJI CCNNGG 1 cut(s) 511
BsaWI WCCGGW 1 cut(s) 166
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse1I ACTGG 3 cut(s) 88, 315, 436
Bse21I CCTNAGG 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 511
BseGI GGATG 2 cut(s) 273, 310
BseLI CCNNNNNNNGG 1 cut(s) 482
BseMII CTCAG 2 cut(s) 226, 518
BseNI ACTGG 3 cut(s) 88, 315, 436
BseRI GAGGAG 2 cut(s) 78, 116
BseXI GCAGC 5 cut(s) 10, 53, 342, 373, 532
BseYI CCCAGC 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 500
BshFI GGCC 1 cut(s) 509
BsiHKAI GWGCWC 1 cut(s) 495
BsiSI CCGG 1 cut(s) 167
BslI CCNNNNNNNGG 1 cut(s) 482
BsmI GAATGC 2 cut(s) 92, 333
BsnI GGCC 1 cut(s) 509
Bsp1286I GDGCHC 1 cut(s) 495
Bsp143I GATC 3 cut(s) 67, 105, 585
Bsp19I CCATGG 1 cut(s) 511
BspANI GGCC 1 cut(s) 509
BspCNI CTCAG 2 cut(s) 225, 517
BspLI GGNNCC 2 cut(s) 135, 171
BspPI GGATC 2 cut(s) 62, 100
BsrI ACTGG 3 cut(s) 88, 315, 436
BssECI CCNNGG 1 cut(s) 511
BssMI GATC 3 cut(s) 67, 105, 585
BssNI GRCGYC 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 511
Bst6I CTCTTC 2 cut(s) 188, 282
BstACI GRCGYC 1 cut(s) 205
BstC8I GCNNGC 3 cut(s) 6, 258, 353
BstDEI CTNAG 2 cut(s) 212, 504
BstDSI CCRYGG 1 cut(s) 511
BstF5I GGATG 2 cut(s) 273, 310
BstKTI GATC 3 cut(s) 70, 108, 588
BstMBI GATC 3 cut(s) 67, 105, 585
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstV1I GCAGC 5 cut(s) 10, 53, 342, 373, 532
BstX2I RGATCY 3 cut(s) 67, 105, 585
BstYI RGATCY 3 cut(s) 67, 105, 585
Bsu36I CCTNAGG 1 cut(s) 504
BsuRI GGCC 1 cut(s) 509
BtgI CCRYGG 1 cut(s) 511
BtsCI GGATG 2 cut(s) 273, 310
BtsI GCAGTG 1 cut(s) 535
BtsIMutI CAGTG 3 cut(s) 81, 429, 535
Cac8I GCNNGC 3 cut(s) 6, 258, 353
Cfr13I GGNCC 1 cut(s) 508
CviAII CATG 2 cut(s) 247, 512
CviJI RGCY 9 cut(s) 16, 23, 136, 256, 341, 355, 493, 509, 523
CviKI_1 RGCY 9 cut(s) 16, 23, 136, 256, 341, 355, 493, 509, 523
DdeI CTNAG 2 cut(s) 212, 504
DpnI GATC 3 cut(s) 69, 107, 587
DpnII GATC 3 cut(s) 67, 105, 585
Eam1104I CTCTTC 2 cut(s) 188, 282
EarI CTCTTC 2 cut(s) 188, 282
Ecl136II GAGCTC 1 cut(s) 493
Eco130I CCWWGG 1 cut(s) 511
Eco24I GRGCYC 1 cut(s) 495
Eco53kI GAGCTC 1 cut(s) 493
Eco57I CTGAAG 1 cut(s) 306
Eco81I CCTNAGG 1 cut(s) 504
EcoICRI GAGCTC 1 cut(s) 493
EcoT14I CCWWGG 1 cut(s) 511
EcoT38I GRGCYC 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 511
FaeI CATG 2 cut(s) 250, 515
FaiI YATR 7 cut(s) 111, 234, 248, 281, 324, 423, 513
FatI CATG 2 cut(s) 246, 511
FblI GTMKAC 1 cut(s) 208
Fnu4HI GCNGC 5 cut(s) 24, 42, 356, 387, 521
FokI GGATG 2 cut(s) 280, 317
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 5 cut(s) 24, 42, 356, 387, 521
GluI GCNGC 5 cut(s) 24, 42, 356, 387, 521
GsaI CCCAGC 1 cut(s) 345
HaeIII GGCC 1 cut(s) 509
HapII CCGG 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 205
Hin1II CATG 2 cut(s) 250, 515
HincII GTYRAC 1 cut(s) 209
HindII GTYRAC 1 cut(s) 209
HinfI GANTC 3 cut(s) 53, 176, 210
HpaII CCGG 1 cut(s) 167
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 2 cut(s) 203, 209
Hpy188I TCNGA 5 cut(s) 98, 181, 375, 556, 590
Hpy188III TCNNGA 4 cut(s) 173, 214, 447, 580
Hpy8I GTNNAC 2 cut(s) 203, 209
Hpy99I CGWCG 1 cut(s) 210
HpyAV CCTTC 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 205
HpyCH4V TGCA 4 cut(s) 26, 41, 271, 481
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 2 cut(s) 212, 504
HpySE526I ACGT 1 cut(s) 205
Hsp92I GRCGYC 1 cut(s) 205
Hsp92II CATG 2 cut(s) 250, 515
Kzo9I GATC 3 cut(s) 67, 105, 585
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 5 cut(s) 10, 53, 342, 373, 532
LweI GCATC 1 cut(s) 258
MaeII ACGT 1 cut(s) 205
MalI GATC 3 cut(s) 69, 107, 587
MboI GATC 3 cut(s) 67, 105, 585
MboII GAAGA 3 cut(s) 205, 229, 299
MflI RGATCY 3 cut(s) 67, 105, 585
MhlI GDGCHC 1 cut(s) 495
MluCI AATT 2 cut(s) 117, 418
MlyI GAGTC 1 cut(s) 204
MnlI CCTC 7 cut(s) 56, 94, 147, 191, 243, 513, 576
MroXI GAANNNNTTC 1 cut(s) 568
MseI TTAA 1 cut(s) 408
MspI CCGG 1 cut(s) 167
Mva1269I GAATGC 2 cut(s) 92, 333
MwoI GCNNNNNNNGC 1 cut(s) 395
NcoI CCATGG 1 cut(s) 511
NdeII GATC 3 cut(s) 67, 105, 585
NlaIII CATG 2 cut(s) 250, 515
NlaIV GGNNCC 2 cut(s) 135, 171
PctI GAATGC 2 cut(s) 92, 333
PdmI GAANNNNTTC 1 cut(s) 568
PfeI GAWTC 2 cut(s) 53, 176
PkrI GCNGC 5 cut(s) 25, 43, 357, 388, 522
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 495
PspFI CCCAGC 1 cut(s) 341
PspN4I GGNNCC 2 cut(s) 135, 171
PspPI GGNCC 1 cut(s) 508
PsuI RGATCY 3 cut(s) 67, 105, 585
SacI GAGCTC 1 cut(s) 495
SalI GTCGAC 1 cut(s) 207
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 5 cut(s) 24, 42, 356, 387, 521
Sau3AI GATC 3 cut(s) 67, 105, 585
Sau96I GGNCC 1 cut(s) 508
SchI GAGTC 1 cut(s) 204
SduI GDGCHC 1 cut(s) 495
SfaNI GCATC 1 cut(s) 258
Sse9I AATT 2 cut(s) 117, 418
SstI GAGCTC 1 cut(s) 495
StyI CCWWGG 1 cut(s) 511
TaiI ACGT 1 cut(s) 208
TaqI TCGA 2 cut(s) 208, 365
TasI AATT 2 cut(s) 117, 418
TfiI GAWTC 2 cut(s) 53, 176
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 3 cut(s) 88, 436, 535
TseI GCWGC 5 cut(s) 23, 41, 355, 386, 520
TspDTI ATGAA 1 cut(s) 236
TspRI CASTG 3 cut(s) 88, 436, 535
XmiI GTMKAC 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 568
ZraI GACGTC 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.