Prupe.6G220500_v2.0.a1

U4 U6 small nuclear ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001559
Physical Location & Seq
Reverse (-)
22618571 .. 22622571
4001 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G220500.2

Sequence Viewer

Length: 1458 bp
ATGGCGACTCTTGCTGATTCATTTCTTGCAGACCTTGATGAACTATCTGACAATGAAGCTGATGTTATTGTTGAAGATGATGCTGATGCTGGAAACATGGAAGAAGATATTGATGGGGACTTGGCAGACTTGGAAACGCTTAATTACGATGATCTGGATAGTGTTTCAAAATTGCAGAAAACGCAAAGATATACTGATATAATGCAGAAAGTGGAAGAGGCTTTGGAGAAGGGTTCTGATATGTCAAGTCATGGAATAGTTTTAGAAGATGATCCAGAATACCAGCTGATTGTGGACTGTAATGCTTTGTCCGTTGACATTGAGAATGAAATTGTAATCATCCACAATTTTATTCGTGATAAGTATCGCCCAAAGTTTCCAGAGCTTGAATCACTTGTGCATCATCCGATTGATTACGCCCGAGTTGTTAAGAAAATTGGGAATGAAATGGATGTAACCCTTGTTGATCTGGAGGGGCTTTTACCTTCTGCAATTATTATGGTTGTGTCAGTGACAGCATCAACTACAAGTGGCAAGCCGCTTCCAGAGGAAGTCCTTACAAAAACAAATGAGGCATGCGATCGAGCCCTTGCTCTTGATTCATCAAAGAAGAAGGTTCTTGATTTTGTTGAAAGTAGAATGGGCTTTATTGCACCAAATCTTTCTGCTATAGTTGGGAGTGCTGTTGCGGCGAAACTAATGGGGACAGCTGGCGGTCTCGTATCCTTAGCTAAGATGCCTGCTTGTAATGTTCAGCTTCTTGGTGCTAAGAGAAAAAACCTTGCTGGGTTTTCCACTGCAACATCACAATTTCGTGTTGGTTATGTTGAGCAGACAGAAATATTTCAAACTACACCCCCTTCTTTGAGGATGCGTGCTTGCCGACTCTTGGCTGCAAAATCAACACTTGCAGCACGGGTGGATTCAACAAGAGGAGATCCATCTGGAAACACTGGAAGGGCATTCCGGGAGGAGATACGTAAGAAAATTGAGAAATGGCAGGAGCCTCCTCCTGCAAAACAACCTAAACCACTTCCGGTTCCAGATTCTGAACCTAAGAAGAAGAGAGGTGGTCGTCGGCTAAGGAAGATGAAGGAAAGATATGCGATAACAGACATGAGGAAGCTGGCAAATAGGATGCAATTTGGTATACCTGAAGAGAGTTCCTTAGGTGATGGACTGGGTGAAGGTTATGGAATGCTTGGTCAAGCTGGGAGTGGCAAGCTGCGTGTATCAATGGGTCAGAGCAAACTTGCTGCCAAAGTTGCTAAGAAGTTCAAGGAGAAGAATTATGGAAGCAGTGGTGCTACATCTGGACTCACTTCAAGTTTAGCATTTACGCCTGTGCAGGGGATCGAGCTCTCAAATCCACAGGCTCATGCACACCAGCTCGGCGGTGGAACTCAAAGCACCTACTTCTCTGAAACGGGAACATTTTCAAAAATCAAGAGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Nop Prp31_C

Protein Analysis

486

Amino Acids

52.76

Weight (kDa)

5.88

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1150
AciI CCGC 4 cut(s) 539, 689, 714, 1395
AclWI GGATC 3 cut(s) 266, 932, 1361
AcuI CTGAAG 1 cut(s) 1176
AfiI CCNNNNNNNGG 2 cut(s) 889, 1349
AgsI TTSAA 9 cut(s) 74, 168, 389, 632, 848, 927, 1279, 1326, 1440
Alw21I GWGCWC 1 cut(s) 1362
Alw26I GTCTC 1 cut(s) 722
AlwI GGATC 3 cut(s) 266, 932, 1361
AlwNI CAGNNNCTG 1 cut(s) 1049
Ama87I CYCGRG 1 cut(s) 420
ApeKI GCWGC 4 cut(s) 893, 911, 1225, 1256
Asp700I GAANNNNTTC 2 cut(s) 843, 1435
AsuC2I CCSGG 1 cut(s) 968
AsuHPI GGTGA 2 cut(s) 1184, 1196
AvaI CYCGRG 1 cut(s) 420
AxyI CCTNAGG 1 cut(s) 1168
BanII GRGCYC 2 cut(s) 589, 1362
Bbv12I GWGCWC 1 cut(s) 1362
BbvI GCAGC 4 cut(s) 880, 923, 1212, 1243
BccI CCATC 3 cut(s) 107, 949, 1169
BciVI GTATCC 1 cut(s) 733
BcnI CCSGG 1 cut(s) 968
BcoDI GTCTC 1 cut(s) 722
BfmI CTRYAG 1 cut(s) 669
BfuI GTATCC 1 cut(s) 733
BisI GCNGC 6 cut(s) 539, 690, 894, 912, 1226, 1257
BlsI GCNGC 6 cut(s) 540, 691, 895, 913, 1227, 1258
Bme1390I CCNGG 1 cut(s) 968
BmeT110I CYCGRG 1 cut(s) 420
BmiI GGNNCC 2 cut(s) 1005, 1041
BmrFI CCNGG 1 cut(s) 968
BmrI ACTGGG 1 cut(s) 1190
BmsI GCATC 7 cut(s) 70, 76, 409, 527, 726, 861, 1128
BmuI ACTGGG 1 cut(s) 1190
BpmI CTGGAG 1 cut(s) 491
Bpu10I CCTNAGC 2 cut(s) 727, 1082
BpuMI CCSGG 1 cut(s) 968
BsaAI YACGTR 1 cut(s) 980
BsaBI GATNNNNATC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 722
BsaWI WCCGGW 1 cut(s) 1036
Bsc4I CCNNNNNNNGG 2 cut(s) 889, 1349
Bse1I ACTGG 2 cut(s) 958, 1185
Bse21I CCTNAGG 1 cut(s) 1168
Bse8I GATNNNNATC 1 cut(s) 363
BseGI GGATG 5 cut(s) 339, 403, 457, 876, 1143
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 2 cut(s) 889, 1349
BseNI ACTGG 2 cut(s) 958, 1185
BseRI GAGGAG 3 cut(s) 948, 986, 999
BseXI GCAGC 4 cut(s) 880, 923, 1212, 1243
BseYI CCCAGC 2 cut(s) 785, 1211
BsgI GTGCAG 1 cut(s) 1367
Bsh1285I CGRYCG 1 cut(s) 583
BsiEI CGRYCG 1 cut(s) 583
BsiHKAI GWGCWC 1 cut(s) 1362
BsiHKCI CYCGRG 1 cut(s) 420
BsiSI CCGG 2 cut(s) 967, 1037
BslFI GGGAC 2 cut(s) 131, 718
BslI CCNNNNNNNGG 2 cut(s) 889, 1349
BsmAI GTCTC 1 cut(s) 722
BsmFI GGGAC 2 cut(s) 131, 718
BsmI GAATGC 2 cut(s) 962, 1203
Bso31I GGTCTC 1 cut(s) 722
BsoBI CYCGRG 1 cut(s) 420
Bsp1286I GDGCHC 2 cut(s) 589, 1362
Bsp143I GATC 7 cut(s) 151, 271, 466, 580, 937, 1353, 1452
BspACI CCGC 4 cut(s) 539, 689, 714, 1395
BspLI GGNNCC 2 cut(s) 1005, 1041
BspPI GGATC 3 cut(s) 266, 932, 1361
BspTNI GGTCTC 1 cut(s) 722
BsrI ACTGG 2 cut(s) 958, 1185
BssMI GATC 7 cut(s) 151, 271, 466, 580, 937, 1353, 1452
BssNAI GTATAC 1 cut(s) 1151
Bst1107I GTATAC 1 cut(s) 1151
Bst4CI ACNGT 1 cut(s) 299
Bst6I CTCTTC 3 cut(s) 210, 1058, 1152
BstBAI YACGTR 1 cut(s) 980
BstC8I GCNNGC 8 cut(s) 536, 577, 712, 741, 876, 880, 1128, 1223
BstDEI CTNAG 7 cut(s) 727, 732, 768, 1056, 1082, 1168, 1269
BstF5I GGATG 5 cut(s) 339, 403, 457, 876, 1143
BstKTI GATC 7 cut(s) 154, 274, 469, 583, 940, 1356, 1455
BstMAI GTCTC 1 cut(s) 722
BstMBI GATC 7 cut(s) 151, 271, 466, 580, 937, 1353, 1452
BstMCI CGRYCG 1 cut(s) 583
BstMWI GCNNNNNNNGC 4 cut(s) 11, 181, 689, 1265
BstNSI RCATGY 1 cut(s) 579
BstSCI CCNGG 1 cut(s) 966
BstSFI CTRYAG 1 cut(s) 669
BstSNI TACGTA 1 cut(s) 980
BstV1I GCAGC 4 cut(s) 880, 923, 1212, 1243
BstX2I RGATCY 2 cut(s) 937, 1452
BstYI RGATCY 2 cut(s) 937, 1452
BstZ17I GTATAC 1 cut(s) 1151
Bsu36I CCTNAGG 1 cut(s) 1168
BsuI GTATCC 1 cut(s) 733
BtsCI GGATG 5 cut(s) 339, 403, 457, 876, 1143
BtsI GCAGTG 2 cut(s) 795, 1306
BtsIMutI CAGTG 4 cut(s) 516, 795, 951, 1306
Cac8I GCNNGC 8 cut(s) 536, 577, 712, 741, 876, 880, 1128, 1223
CaiI CAGNNNCTG 1 cut(s) 1049
CviAII CATG 5 cut(s) 97, 251, 576, 1117, 1379
DdeI CTNAG 7 cut(s) 727, 732, 768, 1056, 1082, 1168, 1269
DpnI GATC 7 cut(s) 153, 273, 468, 582, 939, 1355, 1454
DpnII GATC 7 cut(s) 151, 271, 466, 580, 937, 1353, 1452
Eam1104I CTCTTC 3 cut(s) 210, 1058, 1152
EarI CTCTTC 3 cut(s) 210, 1058, 1152
Ecl136II GAGCTC 1 cut(s) 1360
Eco105I TACGTA 1 cut(s) 980
Eco24I GRGCYC 2 cut(s) 589, 1362
Eco31I GGTCTC 1 cut(s) 722
Eco53kI GAGCTC 1 cut(s) 1360
Eco57I CTGAAG 1 cut(s) 1176
Eco81I CCTNAGG 1 cut(s) 1168
Eco88I CYCGRG 1 cut(s) 420
EcoICRI GAGCTC 1 cut(s) 1360
EcoT38I GRGCYC 2 cut(s) 589, 1362
FaeI CATG 5 cut(s) 100, 254, 579, 1120, 1382
FaqI GGGAC 2 cut(s) 131, 718
FatI CATG 5 cut(s) 96, 250, 575, 1116, 1378
FblI GTMKAC 1 cut(s) 1150
Fnu4HI GCNGC 6 cut(s) 539, 690, 894, 912, 1226, 1257
FokI GGATG 5 cut(s) 326, 390, 464, 883, 1150
FriOI GRGCYC 2 cut(s) 589, 1362
Fsp4HI GCNGC 6 cut(s) 539, 690, 894, 912, 1226, 1257
GluI GCNGC 6 cut(s) 539, 690, 894, 912, 1226, 1257
GsaI CCCAGC 2 cut(s) 789, 1215
GsuI CTGGAG 1 cut(s) 491
HapII CCGG 2 cut(s) 967, 1037
Hin1II CATG 5 cut(s) 100, 254, 579, 1120, 1382
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HinfI GANTC 8 cut(s) 7, 17, 389, 599, 885, 923, 1046, 1317
HpaII CCGG 2 cut(s) 967, 1037
HphI GGTGA 2 cut(s) 1184, 1196
Hpy166II GTNNAC 3 cut(s) 295, 316, 1151
Hpy188I TCNGA 7 cut(s) 49, 238, 408, 1051, 1245, 1423, 1457
Hpy8I GTNNAC 3 cut(s) 295, 316, 1151
Hpy99I CGWCG 1 cut(s) 1080
HpyAV CCTTC 7 cut(s) 223, 495, 607, 870, 951, 1087, 1181
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4IV ACGT 1 cut(s) 979
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 181, 689, 1265
HpyF3I CTNAG 7 cut(s) 727, 732, 768, 1056, 1082, 1168, 1269
HpySE526I ACGT 1 cut(s) 979
Hsp92II CATG 5 cut(s) 100, 254, 579, 1120, 1382
Kzo9I GATC 7 cut(s) 151, 271, 466, 580, 937, 1353, 1452
LmnI GCTCC 1 cut(s) 1003
Lsp1109I GCAGC 4 cut(s) 880, 923, 1212, 1243
LweI GCATC 7 cut(s) 70, 76, 409, 527, 726, 861, 1128
MaeII ACGT 1 cut(s) 979
MaeIII GTNAC 2 cut(s) 454, 511
MalI GATC 7 cut(s) 153, 273, 468, 582, 939, 1355, 1454
MboI GATC 7 cut(s) 151, 271, 466, 580, 937, 1353, 1452
MflI RGATCY 2 cut(s) 937, 1452
MhlI GDGCHC 2 cut(s) 589, 1362
MlyI GAGTC 2 cut(s) 879, 1311
MroXI GAANNNNTTC 2 cut(s) 843, 1435
MseI TTAA 2 cut(s) 141, 429
MspA1I CMGCKG 2 cut(s) 286, 710
MspI CCGG 2 cut(s) 967, 1037
MspR9I CCNGG 1 cut(s) 968
Mva1269I GAATGC 2 cut(s) 962, 1203
MwoI GCNNNNNNNGC 4 cut(s) 11, 181, 689, 1265
NciI CCSGG 1 cut(s) 968
NdeII GATC 7 cut(s) 151, 271, 466, 580, 937, 1353, 1452
NlaIII CATG 5 cut(s) 100, 254, 579, 1120, 1382
NlaIV GGNNCC 2 cut(s) 1005, 1041
NmeAIII GCCGAG 1 cut(s) 1371
NmuCI GTSAC 1 cut(s) 511
NspI RCATGY 1 cut(s) 579
PaeI GCATGC 1 cut(s) 579
PctI GAATGC 2 cut(s) 962, 1203
PdmI GAANNNNTTC 2 cut(s) 843, 1435
PfeI GAWTC 5 cut(s) 17, 389, 599, 923, 1046
PfoI TCCNGGA 1 cut(s) 966
PkrI GCNGC 6 cut(s) 540, 691, 895, 913, 1227, 1258
Ple19I CGATCG 1 cut(s) 583
PleI GAGTC 2 cut(s) 879, 1311
PpsI GAGTC 2 cut(s) 879, 1311
Ppu21I YACGTR 1 cut(s) 980
Psp124BI GAGCTC 1 cut(s) 1362
PspFI CCCAGC 2 cut(s) 785, 1211
PspN4I GGNNCC 2 cut(s) 1005, 1041
PstNI CAGNNNCTG 1 cut(s) 1049
PsuI RGATCY 2 cut(s) 937, 1452
PvuI CGATCG 1 cut(s) 583
PvuII CAGCTG 2 cut(s) 286, 710
SacI GAGCTC 1 cut(s) 1362
SaqAI TTAA 2 cut(s) 141, 429
SatI GCNGC 6 cut(s) 539, 690, 894, 912, 1226, 1257
Sau3AI GATC 7 cut(s) 151, 271, 466, 580, 937, 1353, 1452
SchI GAGTC 2 cut(s) 879, 1311
ScrFI CCNGG 1 cut(s) 968
SduI GDGCHC 2 cut(s) 589, 1362
SfaNI GCATC 7 cut(s) 70, 76, 409, 527, 726, 861, 1128
SfcI CTRYAG 1 cut(s) 669
SnaBI TACGTA 1 cut(s) 980
SphI GCATGC 1 cut(s) 579
SsiI CCGC 4 cut(s) 539, 689, 714, 1395
SspI AATATT 1 cut(s) 843
SstI GAGCTC 1 cut(s) 1362
StyD4I CCNGG 1 cut(s) 966
TaaI ACNGT 1 cut(s) 299
TaiI ACGT 1 cut(s) 982
TaqI TCGA 2 cut(s) 583, 1356
TauI GCSGC 2 cut(s) 541, 692
TfiI GAWTC 5 cut(s) 17, 389, 599, 923, 1046
Tru1I TTAA 2 cut(s) 141, 429
Tru9I TTAA 2 cut(s) 141, 429
TscAI CASTG 4 cut(s) 516, 802, 958, 1306
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 4 cut(s) 893, 911, 1225, 1256
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 7 cut(s) 9, 54, 69, 342, 459, 591, 1106
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 4 cut(s) 516, 802, 958, 1306
XceI RCATGY 1 cut(s) 579
XcmI CCANNNNNNNNNTGG 1 cut(s) 1394
XmiI GTMKAC 1 cut(s) 1150
XmnI GAANNNNTTC 2 cut(s) 843, 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.