RLG00000017635

U4 U6 small nuclear ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001559
Physical Location & Seq
Forward (+)
19366589 .. 19369439
2851 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017635

Sequence Viewer

Length: 1461 bp
ATGGCAACTCTAGCCGATTCGTTTCTTGCTGATCTTGATGAGCTATCTGACAATGAAGCCGATGTTCCTGTTGAAGATGATGCTGATGTTGCAAACATGGAGGAAGATGTCGATGGGGACTTGGCAGACTTAGAGACACTTAACTATGATGATCTGGATAGTGTTTCAAAATTGCAGAAATCACAAAGATTTGCCGATATAATGCAGAAAGTGGAAGGTGCACTTGAGAAGGGTTCCGATATGTCAAATCATGCACTAGTTTTGGAAGATGATCCAGAATATCAGCTGATTGTGGACTGTAATGCTTTGTCAGTTGACATTGAGAATGAACTTGTAATCATCCACAATTTTATTCGTGACAAATACCGGCCAAAATTTCCAGAGCTTGAGTCACTTGTTCATCATCCAATTGATTATGCTCGAGTTGTTCAGAAAATTGGGAATGAAATGGATGTAACCCTTGTTGATCTGGAGGGGCTTTTACCTTCAGCAACTATTATGGTTGTGTCAGTTACAGCTTCAACTACAAGTGGAAAGCCACTTCCAGAGGACGTCCTGGAGAAAATAATTGATGCATGTGATCGAGCCCTTGCTCTCGATTCAGCAAAGAAAAAGGTTCTTGATTTTGTAGAAAGTAGAATGGGTTTTATTGCACCAAATCTTTCTGCTATTGTTGGGAGTGCTGTTGCGGCAAAACTTATGGGGATGGCTGGTGGTCTTGCTGCCTTAGCTAAAATGCCTGCTTGTAATGTTCAGCTTCTTGGTGCTAAGAAAAAAAATCTTGCTGGGTTTTCCACTGCAACATCACAATATCGTATTGGTTATGTTGAGGAGACAGAGATATTTCAAACTACACCCCCTGCTCTTAGGATGCGTGCTGGTCGGCTCTTGGCTGCGAAATCAACCCTTGCAGCACGGGTGGATTCTACGAGAGGAGATCCATCTGGGAACACCGGAAAGGCATTCCGAGAGGAGATCCATAAGAAAATTGAAAAATGGCAGGAGCCTCCCCCTGCAAAGCAACCTAAACCACTTCCAGTTCCTGATTCTGAACCTAAAAAGAAGAGAGGTGGACGTCGACTCAGGAAGATGAAAGAAAGATATGCGATAACAGACATGAGGAAGCTCGCAAACAGGATGCAGTTTGGCATACCTGAAGAGAGTTCTTTAGGGGATGGACTGGGTGAAGGATATGGAATGCTTGGTCAGGCTGGGAGTGGCAAGCTGCGTGTGTCGATGGGTCAGAGCAAACTTGCTGCTAAAGTTGCTAAAAAGTTTAAGGATAAGAATTATGGAAGCACTGGTGGTGCTACATCTGGACTGACTTCAAGTTTGGCATTTACTCCTGTGCAGGGGATTGAGCTCTCAAATCCTCAGGCCCATGGACAGCAGCTTGGCAGTGGAACTCAAAGCACCTATTTCTCTGAAACAGGAACATTTTCAAAAATCAAGAGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Nop Prp31_C

Protein Analysis

487

Amino Acids

52.61

Weight (kDa)

5.54

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop PF01798 104 - 332 6.6e-73 snoRNA binding domain, fibrillarin
Prp31_C PF09785 339 - 458 2.6e-43 Prp31 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 555, 1078
AccI GTMKAC 1 cut(s) 1078
AciI CCGC 1 cut(s) 689
AclWI GGATC 3 cut(s) 266, 932, 970
AcoI YGGCCR 1 cut(s) 368
AcsI RAATTY 1 cut(s) 374
AcuI CTGAAG 2 cut(s) 471, 1176
AcyI GRCGYC 2 cut(s) 552, 1075
AfiI CCNNNNNNNGG 1 cut(s) 1352
AgsI TTSAA 7 cut(s) 74, 168, 522, 848, 992, 1329, 1443
AhlI ACTAGT 1 cut(s) 256
AjnI CCWGG 1 cut(s) 555
Alw21I GWGCWC 2 cut(s) 223, 1365
Alw26I GTCTC 2 cut(s) 128, 827
Alw44I GTGCAC 1 cut(s) 219
AlwI GGATC 3 cut(s) 266, 932, 970
AlwNI CAGNNNCTG 1 cut(s) 1043
Ama87I CYCGRG 1 cut(s) 420
AoxI GGCC 2 cut(s) 368, 1377
ApaLI GTGCAC 1 cut(s) 219
ApeKI GCWGC 6 cut(s) 722, 893, 911, 1225, 1256, 1390
ApoI RAATTY 1 cut(s) 374
ArsI GACNNNNNNTTYG 2 cut(s) 1315, 1347
Asp700I GAANNNNTTC 1 cut(s) 1438
AspS9I GGNCC 1 cut(s) 1378
AsuHPI GGTGA 1 cut(s) 1196
AvaI CYCGRG 1 cut(s) 420
AxyI CCTNAGG 1 cut(s) 1374
BaeGI GKGCMC 1 cut(s) 223
BanII GRGCYC 2 cut(s) 589, 1365
Bbv12I GWGCWC 2 cut(s) 223, 1365
BbvI GCAGC 6 cut(s) 709, 880, 923, 1212, 1243, 1402
BccI CCATC 5 cut(s) 107, 700, 949, 1169, 1231
BciT130I CCWGG 1 cut(s) 557
BcoDI GTCTC 2 cut(s) 128, 827
BcuI ACTAGT 1 cut(s) 256
BfaI CTAG 2 cut(s) 11, 257
BisI GCNGC 7 cut(s) 690, 723, 894, 912, 1226, 1257, 1391
BlsI GCNGC 7 cut(s) 691, 724, 895, 913, 1227, 1258, 1392
Bme1390I CCNGG 1 cut(s) 557
BmeT110I CYCGRG 1 cut(s) 420
BmgT120I GGNCC 1 cut(s) 1378
BmiI GGNNCC 2 cut(s) 235, 1005
BmrFI CCNGG 1 cut(s) 557
BmrI ACTGGG 1 cut(s) 1190
BmsI GCATC 4 cut(s) 70, 562, 861, 1128
BmuI ACTGGG 1 cut(s) 1190
BpmI CTGGAG 2 cut(s) 491, 578
Bpu10I CCTNAGC 1 cut(s) 727
BpuEI CTTGAG 2 cut(s) 245, 407
BsaHI GRCGYC 2 cut(s) 552, 1075
BsaJI CCNNGG 1 cut(s) 1381
BsaWI WCCGGW 1 cut(s) 953
Bsc4I CCNNNNNNNGG 1 cut(s) 1352
Bse118I RCCGGY 1 cut(s) 366
Bse1I ACTGG 3 cut(s) 1037, 1185, 1306
Bse21I CCTNAGG 1 cut(s) 1374
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 1 cut(s) 1381
BseGI GGATG 7 cut(s) 339, 403, 457, 711, 876, 1143, 1180
BseLI CCNNNNNNNGG 1 cut(s) 1352
BseMII CTCAG 2 cut(s) 1096, 1388
BseNI ACTGG 3 cut(s) 1037, 1185, 1306
BseRI GAGGAG 3 cut(s) 845, 948, 986
BseSI GKGCMC 1 cut(s) 223
BseXI GCAGC 6 cut(s) 709, 880, 923, 1212, 1243, 1402
BseYI CCCAGC 2 cut(s) 785, 1211
BsgI GTGCAG 1 cut(s) 1370
BshFI GGCC 2 cut(s) 370, 1379
BsiHKAI GWGCWC 2 cut(s) 223, 1365
BsiHKCI CYCGRG 1 cut(s) 420
BsiSI CCGG 2 cut(s) 367, 954
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 1352
BsmAI GTCTC 2 cut(s) 128, 827
BsmFI GGGAC 1 cut(s) 131
BsmI GAATGC 2 cut(s) 962, 1203
BsnI GGCC 2 cut(s) 370, 1379
BsoBI CYCGRG 1 cut(s) 420
Bsp1286I GDGCHC 3 cut(s) 223, 589, 1365
Bsp143I GATC 8 cut(s) 31, 151, 271, 466, 580, 937, 975, 1455
Bsp19I CCATGG 1 cut(s) 1381
BspACI CCGC 1 cut(s) 689
BspANI GGCC 2 cut(s) 370, 1379
BspCNI CTCAG 2 cut(s) 1095, 1387
BspLI GGNNCC 2 cut(s) 235, 1005
BspPI GGATC 3 cut(s) 266, 932, 970
BsrFI RCCGGY 1 cut(s) 366
BsrI ACTGG 3 cut(s) 1037, 1185, 1306
BssAI RCCGGY 1 cut(s) 366
BssECI CCNNGG 1 cut(s) 1381
BssMI GATC 8 cut(s) 31, 151, 271, 466, 580, 937, 975, 1455
BssNI GRCGYC 2 cut(s) 552, 1075
BssT1I CCWWGG 1 cut(s) 1381
Bst2UI CCWGG 1 cut(s) 557
Bst4CI ACNGT 1 cut(s) 299
Bst6I CTCTTC 2 cut(s) 1058, 1152
BstACI GRCGYC 2 cut(s) 552, 1075
BstC8I GCNNGC 4 cut(s) 741, 876, 1128, 1223
BstDEI CTNAG 6 cut(s) 130, 727, 768, 866, 1082, 1374
BstDSI CCRYGG 1 cut(s) 1381
BstF5I GGATG 7 cut(s) 339, 403, 457, 711, 876, 1143, 1180
BstKTI GATC 8 cut(s) 34, 154, 274, 469, 583, 940, 978, 1458
BstMAI GTCTC 2 cut(s) 128, 827
BstMBI GATC 8 cut(s) 31, 151, 271, 466, 580, 937, 975, 1455
BstMWI GCNNNNNNNGC 5 cut(s) 11, 89, 689, 728, 1265
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 1 cut(s) 579
BstSCI CCNGG 1 cut(s) 555
BstSLI GKGCMC 1 cut(s) 223
BstV1I GCAGC 6 cut(s) 709, 880, 923, 1212, 1243, 1402
BstX2I RGATCY 3 cut(s) 937, 975, 1455
BstYI RGATCY 3 cut(s) 937, 975, 1455
Bsu36I CCTNAGG 1 cut(s) 1374
BsuRI GGCC 2 cut(s) 370, 1379
BtgI CCRYGG 1 cut(s) 1381
BtsCI GGATG 7 cut(s) 339, 403, 457, 711, 876, 1143, 1180
BtsI GCAGTG 2 cut(s) 795, 1405
BtsIMutI CAGTG 3 cut(s) 795, 1299, 1405
Cac8I GCNNGC 4 cut(s) 741, 876, 1128, 1223
CaiI CAGNNNCTG 1 cut(s) 1043
Cfr10I RCCGGY 1 cut(s) 366
Cfr13I GGNCC 1 cut(s) 1378
CviAII CATG 5 cut(s) 97, 251, 576, 1117, 1382
DdeI CTNAG 6 cut(s) 130, 727, 768, 866, 1082, 1374
DpnI GATC 8 cut(s) 33, 153, 273, 468, 582, 939, 977, 1457
DpnII GATC 8 cut(s) 31, 151, 271, 466, 580, 937, 975, 1455
EaeI YGGCCR 1 cut(s) 368
Eam1104I CTCTTC 2 cut(s) 1058, 1152
EarI CTCTTC 2 cut(s) 1058, 1152
Ecl136II GAGCTC 1 cut(s) 1363
Eco130I CCWWGG 1 cut(s) 1381
Eco24I GRGCYC 2 cut(s) 589, 1365
Eco53kI GAGCTC 1 cut(s) 1363
Eco57I CTGAAG 2 cut(s) 471, 1176
Eco81I CCTNAGG 1 cut(s) 1374
Eco88I CYCGRG 1 cut(s) 420
EcoICRI GAGCTC 1 cut(s) 1363
EcoRII CCWGG 1 cut(s) 555
EcoT14I CCWWGG 1 cut(s) 1381
EcoT22I ATGCAT 1 cut(s) 577
EcoT38I GRGCYC 2 cut(s) 589, 1365
ErhI CCWWGG 1 cut(s) 1381
FaeI CATG 5 cut(s) 100, 254, 579, 1120, 1385
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FaqI GGGAC 1 cut(s) 131
FatI CATG 5 cut(s) 96, 250, 575, 1116, 1381
FblI GTMKAC 1 cut(s) 1078
Fnu4HI GCNGC 7 cut(s) 690, 723, 894, 912, 1226, 1257, 1391
FokI GGATG 7 cut(s) 326, 390, 464, 718, 883, 1150, 1187
FriOI GRGCYC 2 cut(s) 589, 1365
Fsp4HI GCNGC 7 cut(s) 690, 723, 894, 912, 1226, 1257, 1391
FspBI CTAG 2 cut(s) 11, 257
GluI GCNGC 7 cut(s) 690, 723, 894, 912, 1226, 1257, 1391
GsaI CCCAGC 2 cut(s) 789, 1215
GsuI CTGGAG 2 cut(s) 491, 578
HaeIII GGCC 2 cut(s) 370, 1379
HapII CCGG 2 cut(s) 367, 954
Hin1I GRCGYC 2 cut(s) 552, 1075
Hin1II CATG 5 cut(s) 100, 254, 579, 1120, 1385
HincII GTYRAC 2 cut(s) 316, 1079
HindII GTYRAC 2 cut(s) 316, 1079
HinfI GANTC 6 cut(s) 17, 389, 599, 923, 1046, 1080
HpaII CCGG 2 cut(s) 367, 954
HphI GGTGA 1 cut(s) 1196
Hpy166II GTNNAC 5 cut(s) 221, 295, 316, 1073, 1079
Hpy188I TCNGA 8 cut(s) 49, 238, 432, 968, 1051, 1245, 1426, 1460
Hpy8I GTNNAC 5 cut(s) 221, 295, 316, 1073, 1079
Hpy99I CGWCG 1 cut(s) 1080
HpyAV CCTTC 4 cut(s) 209, 223, 495, 1181
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4IV ACGT 2 cut(s) 552, 1075
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 89, 689, 728, 1265
HpyF3I CTNAG 6 cut(s) 130, 727, 768, 866, 1082, 1374
HpySE526I ACGT 2 cut(s) 552, 1075
Hsp92I GRCGYC 2 cut(s) 552, 1075
Hsp92II CATG 5 cut(s) 100, 254, 579, 1120, 1385
Kzo9I GATC 8 cut(s) 31, 151, 271, 466, 580, 937, 975, 1455
LmnI GCTCC 1 cut(s) 1003
Lsp1109I GCAGC 6 cut(s) 709, 880, 923, 1212, 1243, 1402
LweI GCATC 4 cut(s) 70, 562, 861, 1128
MaeI CTAG 2 cut(s) 11, 257
MaeII ACGT 2 cut(s) 552, 1075
MaeIII GTNAC 4 cut(s) 356, 390, 454, 511
MalI GATC 8 cut(s) 33, 153, 273, 468, 582, 939, 977, 1457
MboI GATC 8 cut(s) 31, 151, 271, 466, 580, 937, 975, 1455
MboII GAAGA 6 cut(s) 86, 116, 278, 1075, 1099, 1169
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 3 cut(s) 937, 975, 1455
MhlI GDGCHC 3 cut(s) 223, 589, 1365
MluCI AATT 8 cut(s) 170, 346, 374, 408, 435, 567, 987, 1288
MlyI GAGTC 2 cut(s) 398, 1074
Mph1103I ATGCAT 1 cut(s) 577
MroXI GAANNNNTTC 1 cut(s) 1438
MseI TTAA 2 cut(s) 141, 1278
MspA1I CMGCKG 1 cut(s) 286
MspI CCGG 2 cut(s) 367, 954
MspR9I CCNGG 1 cut(s) 557
MunI CAATTG 1 cut(s) 408
Mva1269I GAATGC 2 cut(s) 962, 1203
MvaI CCWGG 1 cut(s) 557
MwoI GCNNNNNNNGC 5 cut(s) 11, 89, 689, 728, 1265
NcoI CCATGG 1 cut(s) 1381
NdeII GATC 8 cut(s) 31, 151, 271, 466, 580, 937, 975, 1455
NlaIII CATG 5 cut(s) 100, 254, 579, 1120, 1385
NlaIV GGNNCC 2 cut(s) 235, 1005
NmuCI GTSAC 2 cut(s) 356, 390
NsiI ATGCAT 1 cut(s) 577
NspI RCATGY 1 cut(s) 579
PaeR7I CTCGAG 1 cut(s) 420
PctI GAATGC 2 cut(s) 962, 1203
PdmI GAANNNNTTC 1 cut(s) 1438
PfeI GAWTC 4 cut(s) 17, 599, 923, 1046
PfoI TCCNGGA 1 cut(s) 555
PkrI GCNGC 7 cut(s) 691, 724, 895, 913, 1227, 1258, 1392
PleI GAGTC 2 cut(s) 397, 1074
PpsI GAGTC 2 cut(s) 397, 1074
Psp124BI GAGCTC 1 cut(s) 1365
Psp6I CCWGG 1 cut(s) 555
PspFI CCCAGC 2 cut(s) 785, 1211
PspGI CCWGG 1 cut(s) 555
PspN4I GGNNCC 2 cut(s) 235, 1005
PspPI GGNCC 1 cut(s) 1378
PspXI VCTCGAGB 1 cut(s) 420
PstNI CAGNNNCTG 1 cut(s) 1043
PsuI RGATCY 3 cut(s) 937, 975, 1455
PvuII CAGCTG 1 cut(s) 286
SacI GAGCTC 1 cut(s) 1365
SalI GTCGAC 1 cut(s) 1077
SaqAI TTAA 2 cut(s) 141, 1278
SatI GCNGC 7 cut(s) 690, 723, 894, 912, 1226, 1257, 1391
Sau3AI GATC 8 cut(s) 31, 151, 271, 466, 580, 937, 975, 1455
Sau96I GGNCC 1 cut(s) 1378
SchI GAGTC 2 cut(s) 398, 1074
ScrFI CCNGG 1 cut(s) 557
SduI GDGCHC 3 cut(s) 223, 589, 1365
SfaNI GCATC 4 cut(s) 70, 562, 861, 1128
Sfr274I CTCGAG 1 cut(s) 420
SlaI CTCGAG 1 cut(s) 420
SmlI CTYRAG 3 cut(s) 224, 386, 420
SmoI CTYRAG 3 cut(s) 224, 386, 420
SpeI ACTAGT 1 cut(s) 256
Sse9I AATT 8 cut(s) 170, 346, 374, 408, 435, 567, 987, 1288
SsiI CCGC 1 cut(s) 689
SspMI CTAG 2 cut(s) 11, 257
SstI GAGCTC 1 cut(s) 1365
StyD4I CCNGG 1 cut(s) 555
StyI CCWWGG 1 cut(s) 1381
TaaI ACNGT 1 cut(s) 299
TaiI ACGT 2 cut(s) 555, 1078
TaqI TCGA 6 cut(s) 111, 421, 583, 597, 1078, 1235
TasI AATT 8 cut(s) 170, 346, 374, 408, 435, 567, 987, 1288
TauI GCSGC 1 cut(s) 692
TfiI GAWTC 4 cut(s) 17, 599, 923, 1046
Tru1I TTAA 2 cut(s) 141, 1278
Tru9I TTAA 2 cut(s) 141, 1278
TscAI CASTG 3 cut(s) 802, 1306, 1405
TseFI GTSAC 2 cut(s) 356, 390
TseI GCWGC 6 cut(s) 722, 893, 911, 1225, 1256, 1390
Tsp45I GTSAC 2 cut(s) 356, 390
TspDTI ATGAA 5 cut(s) 69, 342, 389, 459, 1106
TspRI CASTG 3 cut(s) 802, 1306, 1405
VneI GTGCAC 1 cut(s) 219
XapI RAATTY 1 cut(s) 374
XceI RCATGY 1 cut(s) 579
XhoI CTCGAG 1 cut(s) 420
XmiI GTMKAC 1 cut(s) 1078
XmnI GAANNNNTTC 1 cut(s) 1438
XspI CTAG 2 cut(s) 11, 257
ZraI GACGTC 2 cut(s) 553, 1076
Zsp2I ATGCAT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.