pycom02g15100

U4 U6 small nuclear ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001559
Physical Location & Seq
Forward (+)
11813939 .. 11816708
2770 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15100.6

Sequence Viewer

Length: 1461 bp
ATGGCAACACTTGCTGATTCATTTCTCGCCGACCTTGATGAACTATCTGACAATGAAGATGATATAATTGTTGAAGATGATGCTGATGCTGGAAATATGGAAGAAGATGTTGATGGGGACTTGGCTGATTTAGAAACACTTAATTACGATGATCTGGACAGTGTTTCAAAATTGCAGAAATCACAAAGATATACAGATATAATACAGAAAGTGGAAGGTGCATTGGAGAAGGGTTCTGATATGTCAAATCATGCAATAGTTTTGGAAGATGATCGATCGGAATACCAGCTGATTGTGGACTGTAATGCTTTGTCTGTTGACATTGAGAATGAAATTGTAATCATCCATAATTTTATTCGGGACAAGTATCGTCCAAAATTTCCAGAGCTGGAATCACTTGTGCATCATCCAATTGATTATGCTCGAGTTGTTAAGAAAATTGGAAATGAAATGGACGTAACCCTTGTTGATCTGGAGGGGCTTCTACCTTCAGCAATTATTATGGTTGTGTCAGTTACAGCATCAACTACAAGTGGCAAGCCGCTTCCAGAGGACGTCCTTGCGAAAATAGTTGAGGCATGCGATCGAGCCCTTGCTCTTGATTCAGCAAAGAAGATGGTGCTTGATTTTGTTGAAAGTAGAATGGGCTTTATTGCGCCAAATCTGTCTGCTATTGTTGGTAGTGCTGTTGCGGCAAAACTTATGGGGACGGCTGGGGGTCTCACATCCTTAGCTAAGATGCCAGCTTGTAATGTTCAGCTTCTTGGTGCTAAGAGAAAAAACCTTGCTGGGTTTTCCACTGCAACATCACACTTCCGCGTTGGTTATGTCGAGCAGACAGAAATATTTCAATCTACACCTCCTTCATTGAGGATGCGTGCATGTCGGCTCTTGGCTGCAAAATCAACACTTGCAGCACGGGTGGATTCCACAAGAGGAGATCCATCCGGGAACACTGGAAGGGCATTCCGGGAGGAGATTCGTAAGAAAATTGAGAAATGGCAGGAGCCTCCTCCTGCAAAGCAACCTAAACCACTTCCAGTTCCTGATTCTGAACCTAAAAAGAAGAGAGGTGGTCGCCGACTAAGAAAGATGAAGGAAAGGTATGCGATAACAGACATGAGGAAACTGGCAAACAGGATGCAATTTGGTATACCAGAAGAGAGTTCGTTAGGGGATGGACTGGGTGAAGGTTATGGAATGCTTGGTCAGGCTGGGAGTGGCAAGCTGCGTGTGTCAATGGGTCAGAGCAAACTTGCTGCAAAAGTTGCTAAGAAGTTCAAGGAAAAGAATTATGGAAGCAGTGGTGCTACGTCTGGACTGACTTCAAGTTTGGCGTTTACACCTGTGCAGGGTATTGAGCTTTCAAATCCACAGGCTCATGCGCAGCAGCTTGGCGGCGGAGCTCAAAGCACCTACTTCTCAGAGACGGGAACATTTTCAAAAATCAAGAGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Nop Prp31_C

Protein Analysis

487

Amino Acids

52.79

Weight (kDa)

5.88

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 558
Acc16I TGCGCA 1 cut(s) 1386
AccI GTMKAC 1 cut(s) 1153
AccII CGCG 1 cut(s) 819
AciI CCGC 5 cut(s) 542, 692, 817, 1398, 1401
AclWI GGATC 1 cut(s) 935
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 1 cut(s) 474
AcyI GRCGYC 1 cut(s) 555
AfiI CCNNNNNNNGG 1 cut(s) 1352
AgsI TTSAA 8 cut(s) 74, 168, 635, 851, 1282, 1329, 1368, 1443
AluBI AGCT 9 cut(s) 289, 388, 734, 746, 760, 1228, 1363, 1393, 1406
AluI AGCT 9 cut(s) 289, 388, 734, 746, 760, 1228, 1363, 1393, 1406
Alw21I GWGCWC 1 cut(s) 1408
Alw26I GTCTC 2 cut(s) 725, 1421
AlwI GGATC 1 cut(s) 935
AlwNI CAGNNNCTG 1 cut(s) 1046
Ama87I CYCGRG 1 cut(s) 423
ApeKI GCWGC 6 cut(s) 896, 914, 1228, 1259, 1387, 1390
ApoI RAATTY 1 cut(s) 377
ArsI GACNNNNNNTTYG 2 cut(s) 1315, 1347
Asp700I GAANNNNTTC 2 cut(s) 846, 1438
AspLEI GCGC 2 cut(s) 658, 1387
AsuC2I CCSGG 2 cut(s) 949, 971
AsuHPI GGTGA 1 cut(s) 1199
AvaI CYCGRG 1 cut(s) 423
BanII GRGCYC 2 cut(s) 592, 1408
Bbv12I GWGCWC 1 cut(s) 1408
BbvI GCAGC 6 cut(s) 883, 926, 1215, 1246, 1399, 1402
BccI CCATC 4 cut(s) 107, 610, 952, 1172
BceAI ACGGC 1 cut(s) 726
BcnI CCSGG 2 cut(s) 949, 971
BcoDI GTCTC 2 cut(s) 725, 1421
BisI GCNGC 9 cut(s) 542, 693, 897, 915, 1229, 1260, 1388, 1391, 1399
BlsI GCNGC 9 cut(s) 543, 694, 898, 916, 1230, 1261, 1389, 1392, 1400
Bme1390I CCNGG 2 cut(s) 949, 971
BmeT110I CYCGRG 1 cut(s) 423
BmiI GGNNCC 1 cut(s) 1008
BmrFI CCNGG 2 cut(s) 949, 971
BmrI ACTGGG 1 cut(s) 1193
BmsI GCATC 7 cut(s) 70, 76, 412, 530, 729, 864, 1131
BmuI ACTGGG 1 cut(s) 1193
BpmI CTGGAG 1 cut(s) 494
Bpu10I CCTNAGC 1 cut(s) 730
BpuMI CCSGG 2 cut(s) 949, 971
Bsa29I ATCGAT 1 cut(s) 274
BsaHI GRCGYC 1 cut(s) 555
BsaI GGTCTC 1 cut(s) 725
Bsc4I CCNNNNNNNGG 1 cut(s) 1352
Bse1I ACTGG 4 cut(s) 961, 1040, 1134, 1188
BseCI ATCGAT 1 cut(s) 274
BseGI GGATG 7 cut(s) 342, 406, 725, 879, 944, 1146, 1183
BseLI CCNNNNNNNGG 1 cut(s) 1352
BseMII CTCAG 1 cut(s) 1437
BseNI ACTGG 4 cut(s) 961, 1040, 1134, 1188
BseRI GAGGAG 3 cut(s) 951, 989, 1002
BseXI GCAGC 6 cut(s) 883, 926, 1215, 1246, 1399, 1402
BseYI CCCAGC 3 cut(s) 713, 788, 1214
BsgI GTGCAG 1 cut(s) 1370
Bsh1236I CGCG 1 cut(s) 819
Bsh1285I CGRYCG 2 cut(s) 278, 586
BshVI ATCGAT 1 cut(s) 274
BsiEI CGRYCG 2 cut(s) 278, 586
BsiHKAI GWGCWC 1 cut(s) 1408
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 2 cut(s) 948, 970
BslFI GGGAC 3 cut(s) 131, 374, 721
BslI CCNNNNNNNGG 1 cut(s) 1352
BsmAI GTCTC 2 cut(s) 725, 1421
BsmBI CGTCTC 1 cut(s) 1421
BsmFI GGGAC 3 cut(s) 131, 374, 721
BsmI GAATGC 2 cut(s) 965, 1206
Bso31I GGTCTC 1 cut(s) 725
BsoBI CYCGRG 1 cut(s) 423
Bsp1286I GDGCHC 2 cut(s) 592, 1408
Bsp143I GATC 7 cut(s) 151, 271, 275, 469, 583, 940, 1455
BspACI CCGC 5 cut(s) 542, 692, 817, 1398, 1401
BspCNI CTCAG 1 cut(s) 1436
BspDI ATCGAT 1 cut(s) 274
BspFNI CGCG 1 cut(s) 819
BspLI GGNNCC 1 cut(s) 1008
BspPI GGATC 1 cut(s) 935
BspTNI GGTCTC 1 cut(s) 725
BsrI ACTGG 4 cut(s) 961, 1040, 1134, 1188
BssMI GATC 7 cut(s) 151, 271, 275, 469, 583, 940, 1455
BssNAI GTATAC 1 cut(s) 1154
BssNI GRCGYC 1 cut(s) 555
Bst1107I GTATAC 1 cut(s) 1154
Bst4CI ACNGT 2 cut(s) 161, 302
Bst6I CTCTTC 2 cut(s) 1061, 1155
BstACI GRCGYC 1 cut(s) 555
BstAPI GCANNNNNTGC 2 cut(s) 11, 1268
BstC8I GCNNGC 5 cut(s) 539, 580, 744, 879, 1226
BstDEI CTNAG 6 cut(s) 730, 735, 771, 1085, 1272, 1423
BstF5I GGATG 7 cut(s) 342, 406, 725, 879, 944, 1146, 1183
BstFNI CGCG 1 cut(s) 819
BstHHI GCGC 2 cut(s) 658, 1387
BstKTI GATC 7 cut(s) 154, 274, 278, 472, 586, 943, 1458
BstMAI GTCTC 2 cut(s) 725, 1421
BstMBI GATC 7 cut(s) 151, 271, 275, 469, 583, 940, 1455
BstMCI CGRYCG 2 cut(s) 278, 586
BstMWI GCNNNNNNNGC 3 cut(s) 11, 692, 1268
BstNSI RCATGY 2 cut(s) 582, 885
BstSCI CCNGG 2 cut(s) 947, 969
BstUI CGCG 1 cut(s) 819
BstV1I GCAGC 6 cut(s) 883, 926, 1215, 1246, 1399, 1402
BstX2I RGATCY 2 cut(s) 940, 1455
BstYI RGATCY 2 cut(s) 940, 1455
BstZ17I GTATAC 1 cut(s) 1154
Bsu15I ATCGAT 1 cut(s) 274
BsuTUI ATCGAT 1 cut(s) 274
BtsCI GGATG 7 cut(s) 342, 406, 725, 879, 944, 1146, 1183
BtsI GCAGTG 2 cut(s) 798, 1309
BtsIMutI CAGTG 4 cut(s) 166, 798, 954, 1309
Cac8I GCNNGC 5 cut(s) 539, 580, 744, 879, 1226
CaiI CAGNNNCTG 1 cut(s) 1046
CfoI GCGC 2 cut(s) 658, 1387
ClaI ATCGAT 1 cut(s) 274
CviAII CATG 5 cut(s) 251, 579, 882, 1120, 1382
DdeI CTNAG 6 cut(s) 730, 735, 771, 1085, 1272, 1423
DpnI GATC 7 cut(s) 153, 273, 277, 471, 585, 942, 1457
DpnII GATC 7 cut(s) 151, 271, 275, 469, 583, 940, 1455
Eam1104I CTCTTC 2 cut(s) 1061, 1155
EarI CTCTTC 2 cut(s) 1061, 1155
EciI GGCGGA 1 cut(s) 1416
Ecl136II GAGCTC 1 cut(s) 1406
Eco24I GRGCYC 2 cut(s) 592, 1408
Eco31I GGTCTC 1 cut(s) 725
Eco53kI GAGCTC 1 cut(s) 1406
Eco57I CTGAAG 1 cut(s) 474
Eco88I CYCGRG 1 cut(s) 423
EcoICRI GAGCTC 1 cut(s) 1406
EcoT38I GRGCYC 2 cut(s) 592, 1408
Esp3I CGTCTC 1 cut(s) 1421
FaeI CATG 5 cut(s) 254, 582, 885, 1123, 1385
FaqI GGGAC 3 cut(s) 131, 374, 721
FatI CATG 5 cut(s) 250, 578, 881, 1119, 1381
FblI GTMKAC 1 cut(s) 1153
Fnu4HI GCNGC 9 cut(s) 542, 693, 897, 915, 1229, 1260, 1388, 1391, 1399
FokI GGATG 7 cut(s) 329, 393, 712, 886, 931, 1153, 1190
FriOI GRGCYC 2 cut(s) 592, 1408
Fsp4HI GCNGC 9 cut(s) 542, 693, 897, 915, 1229, 1260, 1388, 1391, 1399
FspI TGCGCA 1 cut(s) 1386
GlaI GCGC 2 cut(s) 657, 1386
GluI GCNGC 9 cut(s) 542, 693, 897, 915, 1229, 1260, 1388, 1391, 1399
GsaI CCCAGC 3 cut(s) 717, 792, 1218
GsuI CTGGAG 1 cut(s) 494
HapII CCGG 2 cut(s) 948, 970
HhaI GCGC 2 cut(s) 658, 1387
Hin1I GRCGYC 1 cut(s) 555
Hin1II CATG 5 cut(s) 254, 582, 885, 1123, 1385
Hin6I GCGC 2 cut(s) 656, 1385
HinP1I GCGC 2 cut(s) 656, 1385
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 6 cut(s) 17, 392, 602, 926, 979, 1049
HpaII CCGG 2 cut(s) 948, 970
HphI GGTGA 1 cut(s) 1199
Hpy166II GTNNAC 4 cut(s) 298, 319, 1154, 1341
Hpy188I TCNGA 7 cut(s) 49, 238, 280, 1054, 1248, 1426, 1460
Hpy188III TCNNGA 9 cut(s) 155, 359, 383, 473, 548, 599, 1046, 1317, 1450
Hpy8I GTNNAC 4 cut(s) 298, 319, 1154, 1341
HpyAV CCTTC 7 cut(s) 209, 223, 498, 873, 954, 1090, 1184
HpyCH4III ACNGT 2 cut(s) 161, 302
HpyCH4IV ACGT 3 cut(s) 456, 555, 1313
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 692, 1268
HpyF3I CTNAG 6 cut(s) 730, 735, 771, 1085, 1272, 1423
HpySE526I ACGT 3 cut(s) 456, 555, 1313
Hsp92I GRCGYC 1 cut(s) 555
Hsp92II CATG 5 cut(s) 254, 582, 885, 1123, 1385
HspAI GCGC 2 cut(s) 656, 1385
Kzo9I GATC 7 cut(s) 151, 271, 275, 469, 583, 940, 1455
LmnI GCTCC 2 cut(s) 1006, 1403
Lsp1109I GCAGC 6 cut(s) 883, 926, 1215, 1246, 1399, 1402
LweI GCATC 7 cut(s) 70, 76, 412, 530, 729, 864, 1131
MaeII ACGT 3 cut(s) 456, 555, 1313
MaeIII GTNAC 2 cut(s) 457, 514
MalI GATC 7 cut(s) 153, 273, 277, 471, 585, 942, 1457
MboI GATC 7 cut(s) 151, 271, 275, 469, 583, 940, 1455
MboII GAAGA 8 cut(s) 68, 86, 113, 116, 278, 625, 1078, 1172
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 2 cut(s) 940, 1455
MhlI GDGCHC 2 cut(s) 592, 1408
MroXI GAANNNNTTC 2 cut(s) 846, 1438
MseI TTAA 2 cut(s) 141, 432
MspA1I CMGCKG 1 cut(s) 289
MspI CCGG 2 cut(s) 948, 970
MspR9I CCNGG 2 cut(s) 949, 971
MunI CAATTG 1 cut(s) 411
Mva1269I GAATGC 2 cut(s) 965, 1206
MvnI CGCG 1 cut(s) 819
MwoI GCNNNNNNNGC 3 cut(s) 11, 692, 1268
NciI CCSGG 2 cut(s) 949, 971
NdeII GATC 7 cut(s) 151, 271, 275, 469, 583, 940, 1455
NlaIII CATG 5 cut(s) 254, 582, 885, 1123, 1385
NlaIV GGNNCC 1 cut(s) 1008
NsbI TGCGCA 1 cut(s) 1386
NspI RCATGY 2 cut(s) 582, 885
PaeI GCATGC 1 cut(s) 582
PaeR7I CTCGAG 1 cut(s) 423
PctI GAATGC 2 cut(s) 965, 1206
PdmI GAANNNNTTC 2 cut(s) 846, 1438
PfeI GAWTC 6 cut(s) 17, 392, 602, 926, 979, 1049
PfoI TCCNGGA 2 cut(s) 947, 969
PkrI GCNGC 9 cut(s) 543, 694, 898, 916, 1230, 1261, 1389, 1392, 1400
Ple19I CGATCG 2 cut(s) 278, 586
Psp124BI GAGCTC 1 cut(s) 1408
PspFI CCCAGC 3 cut(s) 713, 788, 1214
PspN4I GGNNCC 1 cut(s) 1008
PspXI VCTCGAGB 1 cut(s) 423
PstNI CAGNNNCTG 1 cut(s) 1046
PsuI RGATCY 2 cut(s) 940, 1455
PvuI CGATCG 2 cut(s) 278, 586
PvuII CAGCTG 1 cut(s) 289
SacI GAGCTC 1 cut(s) 1408
SaqAI TTAA 2 cut(s) 141, 432
SatI GCNGC 9 cut(s) 542, 693, 897, 915, 1229, 1260, 1388, 1391, 1399
Sau3AI GATC 7 cut(s) 151, 271, 275, 469, 583, 940, 1455
ScrFI CCNGG 2 cut(s) 949, 971
SduI GDGCHC 2 cut(s) 592, 1408
SfaNI GCATC 7 cut(s) 70, 76, 412, 530, 729, 864, 1131
Sfr274I CTCGAG 1 cut(s) 423
SlaI CTCGAG 1 cut(s) 423
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
SphI GCATGC 1 cut(s) 582
SsiI CCGC 5 cut(s) 542, 692, 817, 1398, 1401
SspI AATATT 1 cut(s) 846
SstI GAGCTC 1 cut(s) 1408
StyD4I CCNGG 2 cut(s) 947, 969
TaaI ACNGT 2 cut(s) 161, 302
TaiI ACGT 3 cut(s) 459, 558, 1316
TaqI TCGA 4 cut(s) 274, 424, 586, 831
TauI GCSGC 3 cut(s) 544, 695, 1401
TfiI GAWTC 6 cut(s) 17, 392, 602, 926, 979, 1049
Tru1I TTAA 2 cut(s) 141, 432
Tru9I TTAA 2 cut(s) 141, 432
TscAI CASTG 4 cut(s) 166, 805, 961, 1309
TseI GCWGC 6 cut(s) 896, 914, 1228, 1259, 1387, 1390
TspDTI ATGAA 7 cut(s) 9, 54, 69, 345, 462, 855, 1109
TspRI CASTG 4 cut(s) 166, 805, 961, 1309
XapI RAATTY 1 cut(s) 377
XceI RCATGY 2 cut(s) 582, 885
XhoI CTCGAG 1 cut(s) 423
XmiI GTMKAC 1 cut(s) 1153
XmnI GAANNNNTTC 2 cut(s) 846, 1438
ZraI GACGTC 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.