Prupe.5G061600_v2.0.a1

U4 U6 small nuclear ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001559
Physical Location & Seq
Reverse (-)
6732283 .. 6737386
5104 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G061600.2

Sequence Viewer

Length: 1530 bp
ATGAGCAGAGCACTGGTTGGATCTGCCAAGGCACCAACTGTTTGGAAGCTGACGCCGCTTGAACCTGAAAGGATGGCGACTCTTGCTGATTCATTTCTTGCAGACCTTGATGAACTATCTGACAATGAAGCTGACGTTATTGTTGAAGATGATGCTGATGCTGAAAACATGGAAGAAGATGTTGATGGGGACTTGGCAGACTTGGAAACACTTAATTACGATGATCTGGATAGTGTTTCAAAATTACAAAAAACACAAAGATATGCTGATATAATTCAGAAAGTGGAAGAGGCACTGGTGAAGGGTTCTGATATGTCAAGTCATGGAATAGTTTTAGAAGATGATCCAGAATACCAGCTGATTGTGGACTGTAATGCTTTGTCAGTTGACATTGAGAATGAAATTGTAATCATCCACAATTTTATTCGTGATAAGTATCGCCCAAAGTTTCCAGAGCTTGAATCACTTGTGCATCATCCAATTGATTATGCCCGAGTTGTTAAGAAAATTGGGAATGAAATGGATGTAACCCTTGTTGATCTGGAGGGGCTTTTGCCTTCCGCAATTATTATGGTTGTGTCAGTTACAGCATCAACTACAAGTGGCAAACCGCTTCCAGAGGAAGTCCTTACAAAAACAAATGAGGCATGCGATCGAGCCCTTGCGCTTGATTCAGCAAAGAAGAAGGTTCTTGATTTCGTTGAAAGTAGAATGGGCTTTATTGCACCAAACCTTTCTGCTATAGTTGGGAGTGCTGTTGCGGCAAAACTTATGGGGACAGCCGGCGGTCTCACATCCTTAGCTAAGATGCCTGCTTGTAATGTTCAGCTTCTTGGTGCTAAGAGAAAAAACCTTGCTGGGTTTTCCACTGCAACATCACAATTTCGTGTTGGTTATGTTGAACAGACAGAAATATTTCAAACTACACCCCCTTCTTTGAGGATGCGTGCCTGCCGGCTCTTGGCTGCAAAATCAACACTTGCAGCACGGGTGGATTCCACAAGAGGAGATTCATCTGGAAACACTGGAAGGGCATTCCGGGAGGAGATTCGTAAGAAAATTGAGAAATGGCAGGAGCCTCCTCCTGCAAAACAACCTAAACCACTTCCGGTTCCTGATTCTGAACCTAAAAAGAAGAGAGGTGGTCGCCGGTTAAGGAAGATGAAGGAAAGATATGCGATAACGGACATGAGGAAGCTGGCAAATAGGATGCAATTTGGTATACCTGAAGAGAGTTCCTTAGGGGATGGACTGGGTGAAGGTTATGGAATGCTTGGTCAAGCTGGGAGTGGCAAGCTGCGTGTATCAATGGGTCAGAGCAAACTTGCTGCCAAAGTCGCTAAGAAGTTCAAGGAAAAGAATTATGGAAGCAGTGGTGCTACATCTGGACTTACTTCAAGTTTAGCATTTACGCCTGTGCAGGGGATTGAGCTCTCGAATCCACAGGCTCATGCACACCAGCTCGGCGGTGGAACTCAAAGCACCTACTTCTCTGAAACGGGAACATTTTCAAAAATCAAGAGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Nop Prp31_C

Protein Analysis

510

Amino Acids

55.34

Weight (kDa)

6.2

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 31
AccI GTMKAC 1 cut(s) 1222
AciI CCGC 6 cut(s) 56, 561, 611, 761, 786, 1467
AclWI GGATC 2 cut(s) 28, 338
AcuI CTGAAG 1 cut(s) 1248
AcyI GRCGYC 1 cut(s) 53
AfiI CCNNNNNNNGG 2 cut(s) 961, 1421
Alw21I GWGCWC 2 cut(s) 13, 1434
Alw26I GTCTC 1 cut(s) 794
AlwI GGATC 2 cut(s) 28, 338
Ama87I CYCGRG 1 cut(s) 492
ApeKI GCWGC 4 cut(s) 965, 983, 1297, 1328
Asp700I GAANNNNTTC 2 cut(s) 915, 1507
AspLEI GCGC 1 cut(s) 667
AsuC2I CCSGG 1 cut(s) 1040
AsuHPI GGTGA 2 cut(s) 310, 1268
AvaI CYCGRG 1 cut(s) 492
AxyI CCTNAGG 1 cut(s) 1240
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 2 cut(s) 661, 1434
Bbv12I GWGCWC 2 cut(s) 13, 1434
BbvI GCAGC 4 cut(s) 952, 995, 1284, 1315
BccI CCATC 3 cut(s) 67, 179, 1241
BcnI CCSGG 1 cut(s) 1040
BcoDI GTCTC 1 cut(s) 794
BfmI CTRYAG 1 cut(s) 741
BisI GCNGC 6 cut(s) 56, 762, 966, 984, 1298, 1329
BlsI GCNGC 6 cut(s) 57, 763, 967, 985, 1299, 1330
Bme1390I CCNGG 1 cut(s) 1040
BmeT110I CYCGRG 1 cut(s) 492
BmiI GGNNCC 3 cut(s) 33, 1077, 1113
BmrFI CCNGG 1 cut(s) 1040
BmrI ACTGGG 1 cut(s) 1262
BmsI GCATC 7 cut(s) 142, 148, 481, 599, 798, 933, 1200
BmuI ACTGGG 1 cut(s) 1262
BpmI CTGGAG 1 cut(s) 563
Bpu10I CCTNAGC 1 cut(s) 799
BpuMI CCSGG 1 cut(s) 1040
BsaBI GATNNNNATC 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 794
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 1108
Bsc4I CCNNNNNNNGG 2 cut(s) 961, 1421
Bse118I RCCGGY 3 cut(s) 782, 954, 1149
Bse1I ACTGG 4 cut(s) 18, 300, 1030, 1257
Bse21I CCTNAGG 1 cut(s) 1240
Bse8I GATNNNNATC 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 8 cut(s) 78, 411, 475, 529, 794, 948, 1215, 1252
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 2 cut(s) 961, 1421
BseNI ACTGG 4 cut(s) 18, 300, 1030, 1257
BseRI GAGGAG 3 cut(s) 1020, 1058, 1071
BseXI GCAGC 4 cut(s) 952, 995, 1284, 1315
BseYI CCCAGC 2 cut(s) 857, 1283
BsgI GTGCAG 1 cut(s) 1439
Bsh1285I CGRYCG 1 cut(s) 655
BshNI GGYRCC 1 cut(s) 31
BsiEI CGRYCG 1 cut(s) 655
BsiHKAI GWGCWC 2 cut(s) 13, 1434
BsiHKCI CYCGRG 1 cut(s) 492
BsiSI CCGG 5 cut(s) 783, 955, 1039, 1109, 1150
BslFI GGGAC 2 cut(s) 203, 790
BslI CCNNNNNNNGG 2 cut(s) 961, 1421
BsmAI GTCTC 1 cut(s) 794
BsmFI GGGAC 2 cut(s) 203, 790
BsmI GAATGC 2 cut(s) 1034, 1275
Bso31I GGTCTC 1 cut(s) 794
BsoBI CYCGRG 1 cut(s) 492
Bsp1286I GDGCHC 3 cut(s) 13, 661, 1434
Bsp143I GATC 6 cut(s) 20, 223, 343, 538, 652, 1524
BspACI CCGC 6 cut(s) 56, 561, 611, 761, 786, 1467
BspLI GGNNCC 3 cut(s) 33, 1077, 1113
BspPI GGATC 2 cut(s) 28, 338
BspT107I GGYRCC 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 794
BsrFI RCCGGY 3 cut(s) 782, 954, 1149
BsrI ACTGG 4 cut(s) 18, 300, 1030, 1257
BssAI RCCGGY 3 cut(s) 782, 954, 1149
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 6 cut(s) 20, 223, 343, 538, 652, 1524
BssNAI GTATAC 1 cut(s) 1223
BssNI GRCGYC 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 27
Bst1107I GTATAC 1 cut(s) 1223
Bst4CI ACNGT 2 cut(s) 40, 371
Bst6I CTCTTC 3 cut(s) 282, 1130, 1224
BstACI GRCGYC 1 cut(s) 53
BstC8I GCNNGC 8 cut(s) 649, 784, 813, 948, 952, 956, 1200, 1295
BstDEI CTNAG 5 cut(s) 799, 804, 840, 1240, 1341
BstF5I GGATG 8 cut(s) 78, 411, 475, 529, 794, 948, 1215, 1252
BstHHI GCGC 1 cut(s) 667
BstKTI GATC 6 cut(s) 23, 226, 346, 541, 655, 1527
BstMAI GTCTC 1 cut(s) 794
BstMBI GATC 6 cut(s) 20, 223, 343, 538, 652, 1524
BstMCI CGRYCG 1 cut(s) 655
BstMWI GCNNNNNNNGC 4 cut(s) 55, 83, 761, 1337
BstNSI RCATGY 1 cut(s) 651
BstSCI CCNGG 1 cut(s) 1038
BstSFI CTRYAG 1 cut(s) 741
BstV1I GCAGC 4 cut(s) 952, 995, 1284, 1315
BstX2I RGATCY 2 cut(s) 20, 1524
BstXI CCANNNNNNTGG 1 cut(s) 42
BstYI RGATCY 2 cut(s) 20, 1524
BstZ17I GTATAC 1 cut(s) 1223
Bsu36I CCTNAGG 1 cut(s) 1240
BtsCI GGATG 8 cut(s) 78, 411, 475, 529, 794, 948, 1215, 1252
BtsI GCAGTG 2 cut(s) 867, 1378
BtsIMutI CAGTG 5 cut(s) 11, 293, 867, 1023, 1378
Cac8I GCNNGC 8 cut(s) 649, 784, 813, 948, 952, 956, 1200, 1295
CfoI GCGC 1 cut(s) 667
Cfr10I RCCGGY 3 cut(s) 782, 954, 1149
CseI GACGC 1 cut(s) 61
CviAII CATG 5 cut(s) 169, 323, 648, 1189, 1451
DdeI CTNAG 5 cut(s) 799, 804, 840, 1240, 1341
DpnI GATC 6 cut(s) 22, 225, 345, 540, 654, 1526
DpnII GATC 6 cut(s) 20, 223, 343, 538, 652, 1524
Eam1104I CTCTTC 3 cut(s) 282, 1130, 1224
EarI CTCTTC 3 cut(s) 282, 1130, 1224
Ecl136II GAGCTC 1 cut(s) 1432
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 2 cut(s) 661, 1434
Eco31I GGTCTC 1 cut(s) 794
Eco53kI GAGCTC 1 cut(s) 1432
Eco57I CTGAAG 1 cut(s) 1248
Eco81I CCTNAGG 1 cut(s) 1240
Eco88I CYCGRG 1 cut(s) 492
EcoICRI GAGCTC 1 cut(s) 1432
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 2 cut(s) 661, 1434
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 5 cut(s) 172, 326, 651, 1192, 1454
FaqI GGGAC 2 cut(s) 203, 790
FatI CATG 5 cut(s) 168, 322, 647, 1188, 1450
FblI GTMKAC 1 cut(s) 1222
Fnu4HI GCNGC 6 cut(s) 56, 762, 966, 984, 1298, 1329
FokI GGATG 8 cut(s) 85, 398, 462, 536, 781, 955, 1222, 1259
FriOI GRGCYC 2 cut(s) 661, 1434
Fsp4HI GCNGC 6 cut(s) 56, 762, 966, 984, 1298, 1329
GlaI GCGC 1 cut(s) 666
GluI GCNGC 6 cut(s) 56, 762, 966, 984, 1298, 1329
GsaI CCCAGC 2 cut(s) 861, 1287
GsuI CTGGAG 1 cut(s) 563
HapII CCGG 5 cut(s) 783, 955, 1039, 1109, 1150
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 667
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 5 cut(s) 172, 326, 651, 1192, 1454
Hin6I GCGC 1 cut(s) 665
HinP1I GCGC 1 cut(s) 665
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 9 cut(s) 79, 89, 461, 671, 995, 1010, 1048, 1118, 1438
HpaII CCGG 5 cut(s) 783, 955, 1039, 1109, 1150
HphI GGTGA 2 cut(s) 310, 1268
Hpy166II GTNNAC 3 cut(s) 367, 388, 1223
Hpy188I TCNGA 7 cut(s) 121, 279, 310, 1123, 1317, 1495, 1529
Hpy8I GTNNAC 3 cut(s) 367, 388, 1223
HpyAV CCTTC 7 cut(s) 295, 567, 679, 942, 1023, 1159, 1253
HpyCH4III ACNGT 2 cut(s) 40, 371
HpyCH4IV ACGT 1 cut(s) 135
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 83, 761, 1337
HpyF3I CTNAG 5 cut(s) 799, 804, 840, 1240, 1341
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 5 cut(s) 172, 326, 651, 1192, 1454
HspAI GCGC 1 cut(s) 665
KroI GCCGGC 2 cut(s) 782, 954
KroNI GCCGGC 2 cut(s) 784, 956
Kzo9I GATC 6 cut(s) 20, 223, 343, 538, 652, 1524
LmnI GCTCC 1 cut(s) 1075
Lsp1109I GCAGC 4 cut(s) 952, 995, 1284, 1315
LweI GCATC 7 cut(s) 142, 148, 481, 599, 798, 933, 1200
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 2 cut(s) 526, 583
MalI GATC 6 cut(s) 22, 225, 345, 540, 654, 1526
MboI GATC 6 cut(s) 20, 223, 343, 538, 652, 1524
MboII GAAGA 9 cut(s) 158, 185, 188, 299, 350, 694, 1147, 1171, 1241
MfeI CAATTG 1 cut(s) 480
MflI RGATCY 2 cut(s) 20, 1524
MhlI GDGCHC 3 cut(s) 13, 661, 1434
MlyI GAGTC 1 cut(s) 73
MroNI GCCGGC 2 cut(s) 782, 954
MroXI GAANNNNTTC 2 cut(s) 915, 1507
MseI TTAA 3 cut(s) 213, 501, 1154
MspA1I CMGCKG 1 cut(s) 358
MspI CCGG 5 cut(s) 783, 955, 1039, 1109, 1150
MspR9I CCNGG 1 cut(s) 1040
MunI CAATTG 1 cut(s) 480
Mva1269I GAATGC 2 cut(s) 1034, 1275
MwoI GCNNNNNNNGC 4 cut(s) 55, 83, 761, 1337
NaeI GCCGGC 2 cut(s) 784, 956
NciI CCSGG 1 cut(s) 1040
NdeII GATC 6 cut(s) 20, 223, 343, 538, 652, 1524
NgoMIV GCCGGC 2 cut(s) 782, 954
NlaIII CATG 5 cut(s) 172, 326, 651, 1192, 1454
NlaIV GGNNCC 3 cut(s) 33, 1077, 1113
NmeAIII GCCGAG 1 cut(s) 1443
NspI RCATGY 1 cut(s) 651
PaeI GCATGC 1 cut(s) 651
PctI GAATGC 2 cut(s) 1034, 1275
PdiI GCCGGC 2 cut(s) 784, 956
PdmI GAANNNNTTC 2 cut(s) 915, 1507
PfeI GAWTC 8 cut(s) 89, 461, 671, 995, 1010, 1048, 1118, 1438
PfoI TCCNGGA 1 cut(s) 1038
PkrI GCNGC 6 cut(s) 57, 763, 967, 985, 1299, 1330
Ple19I CGATCG 1 cut(s) 655
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
Psp124BI GAGCTC 1 cut(s) 1434
PspFI CCCAGC 2 cut(s) 857, 1283
PspN4I GGNNCC 3 cut(s) 33, 1077, 1113
PsuI RGATCY 2 cut(s) 20, 1524
PvuI CGATCG 1 cut(s) 655
PvuII CAGCTG 1 cut(s) 358
SacI GAGCTC 1 cut(s) 1434
SaqAI TTAA 3 cut(s) 213, 501, 1154
SatI GCNGC 6 cut(s) 56, 762, 966, 984, 1298, 1329
Sau3AI GATC 6 cut(s) 20, 223, 343, 538, 652, 1524
SchI GAGTC 1 cut(s) 73
ScrFI CCNGG 1 cut(s) 1040
SduI GDGCHC 3 cut(s) 13, 661, 1434
SfaNI GCATC 7 cut(s) 142, 148, 481, 599, 798, 933, 1200
SfcI CTRYAG 1 cut(s) 741
SphI GCATGC 1 cut(s) 651
SsiI CCGC 6 cut(s) 56, 561, 611, 761, 786, 1467
SspI AATATT 1 cut(s) 915
SstI GAGCTC 1 cut(s) 1434
StyD4I CCNGG 1 cut(s) 1038
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 2 cut(s) 40, 371
TaiI ACGT 1 cut(s) 138
TaqI TCGA 2 cut(s) 655, 1436
TauI GCSGC 2 cut(s) 58, 764
TfiI GAWTC 8 cut(s) 89, 461, 671, 995, 1010, 1048, 1118, 1438
Tru1I TTAA 3 cut(s) 213, 501, 1154
Tru9I TTAA 3 cut(s) 213, 501, 1154
TscAI CASTG 5 cut(s) 18, 300, 874, 1030, 1378
TseI GCWGC 4 cut(s) 965, 983, 1297, 1328
TspDTI ATGAA 7 cut(s) 81, 126, 141, 414, 531, 1002, 1178
TspGWI ACGGA 1 cut(s) 1199
TspRI CASTG 5 cut(s) 18, 300, 874, 1030, 1378
XceI RCATGY 1 cut(s) 651
XcmI CCANNNNNNNNNTGG 1 cut(s) 1466
XmiI GTMKAC 1 cut(s) 1222
XmnI GAANNNNTTC 2 cut(s) 915, 1507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.