Rroxscaffold_2G00144490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001559
Physical Location & Seq
Reverse (-)
82375316 .. 82383320
8005 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144490.1

Sequence Viewer

Length: 609 bp
ATGGGAACGATCGGCCGTGCATCGCCGAAAAGTCGTTTTCCGGCCGGGTCGTTGCACGAAGCGACCTTCTTCGATCCACGCCTTGGAGCGGCGGCGGGCAAGATGATGGCACGGGGAGGTTGGGTGGCCTGGTCCGAGGGTGGTGGAGGCCGGGTCGGTGCAGGAGGAGGTATCAAGATGCATGATAAGCGAAGTCTCGCTACCCCACATTCCACTACTCGATTGGCATTAATAGTCAACAAGTCCGGAGTTCCATCGCCAATCGCCACCATCATACCAAGCTCGCCGGTTGGAGGTGGATTGGAGGCCCGATATCGGGTGAAGGAAGTCGAGACGAGACTATCGGTTTCACCCAACAAGGAGCTTGGAGGCCCGGCATCGGTGAAGGAATCCCGAGGCGAGGCTATCGATTTCACCCAACAAGGCCGGCCTGCTCGAGCTAAGCTTGGGTCTCACCCCAATCTCCTCTGTTTTTCTTGCTTCGACACCTGGGGTGGTTCTCAGTGCTATCGTGTCTATGATACCTCACTCCACCAAGAGGAGTTGACGGTTATGAAATTGGGTTTGTCAGAGGGTTGGGTGTATGTGATTAGTGAGGCTGTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

21.36

Weight (kDa)

9.54

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 83
AccBSI CCGCTC 1 cut(s) 89
AccIII TCCGGA 1 cut(s) 245
AciI CCGC 3 cut(s) 89, 92, 95
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 2 cut(s) 13, 42
AfiI CCNNNNNNNGG 8 cut(s) 83, 88, 293, 315, 316, 379, 400, 538
AjnI CCWGG 2 cut(s) 128, 488
AluBI AGCT 4 cut(s) 282, 364, 440, 445
AluI AGCT 4 cut(s) 282, 364, 440, 445
Alw26I GTCTC 4 cut(s) 200, 326, 331, 456
AlwI GGATC 1 cut(s) 68
Ama87I CYCGRG 2 cut(s) 393, 435
Aor13HI TCCGGA 1 cut(s) 245
AoxI GGCC 8 cut(s) 13, 42, 126, 148, 306, 370, 424, 428
AseI ATTAAT 1 cut(s) 230
AspS9I GGNCC 3 cut(s) 132, 307, 371
AsuC2I CCSGG 3 cut(s) 46, 152, 374
AsuHPI GGTGA 5 cut(s) 331, 342, 394, 406, 446
AvaI CYCGRG 2 cut(s) 393, 435
AvaII GGWCC 1 cut(s) 132
BarI GAAGNNNNNNTAC 2 cut(s) 184, 216
BccI CCATC 3 cut(s) 100, 262, 278
BciT130I CCWGG 2 cut(s) 130, 490
BcnI CCSGG 3 cut(s) 46, 152, 374
BcoDI GTCTC 4 cut(s) 200, 326, 331, 456
BisI GCNGC 2 cut(s) 90, 93
BlpI GCTNAGC 1 cut(s) 441
BlsI GCNGC 2 cut(s) 91, 94
Bme1390I CCNGG 5 cut(s) 46, 130, 152, 374, 490
Bme18I GGWCC 1 cut(s) 132
BmeT110I CYCGRG 2 cut(s) 393, 435
BmgT120I GGNCC 3 cut(s) 132, 307, 371
BmrFI CCNGG 5 cut(s) 46, 130, 152, 374, 490
BmsI GCATC 3 cut(s) 29, 168, 386
Bpu1102I GCTNAGC 1 cut(s) 441
BpuMI CCSGG 3 cut(s) 46, 152, 374
Bsa29I ATCGAT 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 456
BsaJI CCNNGG 4 cut(s) 82, 135, 394, 489
BsaWI WCCGGW 1 cut(s) 245
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 138, 168
Bsc4I CCNNNNNNNGG 8 cut(s) 83, 88, 293, 315, 316, 379, 400, 538
Bse118I RCCGGY 2 cut(s) 286, 426
BseAI TCCGGA 1 cut(s) 245
BseBI CCWGG 2 cut(s) 130, 490
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 4 cut(s) 82, 135, 394, 489
BseLI CCNNNNNNNGG 8 cut(s) 83, 88, 293, 315, 316, 379, 400, 538
BseMII CTCAG 1 cut(s) 515
BseRI GAGGAG 3 cut(s) 180, 455, 554
BseX3I CGGCCG 2 cut(s) 13, 42
BsgI GTGCAG 1 cut(s) 180
Bsh1285I CGRYCG 3 cut(s) 12, 16, 45
BshFI GGCC 8 cut(s) 15, 44, 128, 150, 308, 372, 426, 430
BshVI ATCGAT 1 cut(s) 408
BsiEI CGRYCG 3 cut(s) 12, 16, 45
BsiHKCI CYCGRG 2 cut(s) 393, 435
BsiSI CCGG 7 cut(s) 41, 45, 151, 246, 287, 374, 427
BslI CCNNNNNNNGG 8 cut(s) 83, 88, 293, 315, 316, 379, 400, 538
BsmAI GTCTC 4 cut(s) 200, 326, 331, 456
BsmBI CGTCTC 1 cut(s) 326
BsnI GGCC 8 cut(s) 15, 44, 128, 150, 308, 372, 426, 430
Bso31I GGTCTC 1 cut(s) 456
BsoBI CYCGRG 2 cut(s) 393, 435
Bsp13I TCCGGA 1 cut(s) 245
Bsp143I GATC 2 cut(s) 9, 73
Bsp1720I GCTNAGC 1 cut(s) 441
BspACI CCGC 3 cut(s) 89, 92, 95
BspANI GGCC 8 cut(s) 15, 44, 128, 150, 308, 372, 426, 430
BspCNI CTCAG 1 cut(s) 514
BspDI ATCGAT 1 cut(s) 408
BspEI TCCGGA 1 cut(s) 245
BspPI GGATC 1 cut(s) 68
BspTNI GGTCTC 1 cut(s) 456
BsrBI CCGCTC 1 cut(s) 89
BsrFI RCCGGY 2 cut(s) 286, 426
BssAI RCCGGY 2 cut(s) 286, 426
BssECI CCNNGG 4 cut(s) 82, 135, 394, 489
BssMI GATC 2 cut(s) 9, 73
BssT1I CCWWGG 1 cut(s) 82
Bst2UI CCWGG 2 cut(s) 130, 490
Bst4CI ACNGT 1 cut(s) 550
BstC8I GCNNGC 4 cut(s) 97, 284, 428, 432
BstDEI CTNAG 3 cut(s) 441, 501, 606
BstKTI GATC 2 cut(s) 12, 76
BstMAI GTCTC 4 cut(s) 200, 326, 331, 456
BstMBI GATC 2 cut(s) 9, 73
BstMCI CGRYCG 3 cut(s) 12, 16, 45
BstMWI GCNNNNNNNGC 1 cut(s) 187
BstNI CCWGG 2 cut(s) 130, 490
BstSCI CCNGG 5 cut(s) 44, 128, 150, 372, 488
BstZI CGGCCG 2 cut(s) 13, 42
Bsu15I ATCGAT 1 cut(s) 408
BsuRI GGCC 8 cut(s) 15, 44, 128, 150, 308, 372, 426, 430
BsuTUI ATCGAT 1 cut(s) 408
BtgZI GCGATG 2 cut(s) 6, 240
BtsIMutI CAGTG 1 cut(s) 509
Cac8I GCNNGC 4 cut(s) 97, 284, 428, 432
Cfr10I RCCGGY 2 cut(s) 286, 426
Cfr13I GGNCC 3 cut(s) 132, 307, 371
ClaI ATCGAT 1 cut(s) 408
CviAII CATG 1 cut(s) 182
DdeI CTNAG 3 cut(s) 441, 501, 606
DpnI GATC 2 cut(s) 11, 75
DpnII GATC 2 cut(s) 9, 73
EaeI YGGCCR 2 cut(s) 13, 42
EagI CGGCCG 2 cut(s) 13, 42
EclXI CGGCCG 2 cut(s) 13, 42
Eco130I CCWWGG 1 cut(s) 82
Eco31I GGTCTC 1 cut(s) 456
Eco32I GATATC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 132
Eco52I CGGCCG 2 cut(s) 13, 42
Eco88I CYCGRG 2 cut(s) 393, 435
EcoRII CCWGG 2 cut(s) 128, 488
EcoRV GATATC 1 cut(s) 314
EcoT14I CCWWGG 1 cut(s) 82
EcoT22I ATGCAT 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 82
Esp3I CGTCTC 1 cut(s) 326
FaeI CATG 1 cut(s) 185
FaiI YATR 5 cut(s) 183, 275, 519, 554, 585
FatI CATG 1 cut(s) 181
FauI CCCGC 1 cut(s) 88
Fnu4HI GCNGC 2 cut(s) 90, 93
FseI GGCCGGCC 1 cut(s) 430
Fsp4HI GCNGC 2 cut(s) 90, 93
GluI GCNGC 2 cut(s) 90, 93
HaeIII GGCC 8 cut(s) 15, 44, 128, 150, 308, 372, 426, 430
HapII CCGG 7 cut(s) 41, 45, 151, 246, 287, 374, 427
Hin1II CATG 1 cut(s) 185
HincII GTYRAC 2 cut(s) 238, 546
HindII GTYRAC 2 cut(s) 238, 546
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 1 cut(s) 389
HpaII CCGG 7 cut(s) 41, 45, 151, 246, 287, 374, 427
HphI GGTGA 5 cut(s) 331, 342, 394, 406, 446
Hpy166II GTNNAC 2 cut(s) 238, 546
Hpy188I TCNGA 2 cut(s) 136, 571
Hpy188III TCNNGA 4 cut(s) 175, 246, 331, 393
Hpy8I GTNNAC 2 cut(s) 238, 546
HpyAV CCTTC 3 cut(s) 76, 316, 379
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4V TGCA 4 cut(s) 20, 55, 161, 181
HpyF10VI GCNNNNNNNGC 1 cut(s) 187
HpyF3I CTNAG 3 cut(s) 441, 501, 606
Hsp92II CATG 1 cut(s) 185
Kpn2I TCCGGA 1 cut(s) 245
KroI GCCGGC 1 cut(s) 426
KroNI GCCGGC 1 cut(s) 428
Kzo9I GATC 2 cut(s) 9, 73
LmnI GCTCC 2 cut(s) 86, 361
LweI GCATC 3 cut(s) 29, 168, 386
MalI GATC 2 cut(s) 11, 75
MbiI CCGCTC 1 cut(s) 89
MboI GATC 2 cut(s) 9, 73
MboII GAAGA 1 cut(s) 61
MluCI AATT 1 cut(s) 557
MmeI TCCRAC 1 cut(s) 271
Mph1103I ATGCAT 1 cut(s) 183
MroI TCCGGA 1 cut(s) 245
MroNI GCCGGC 1 cut(s) 426
MseI TTAA 1 cut(s) 230
MspI CCGG 7 cut(s) 41, 45, 151, 246, 287, 374, 427
MspR9I CCNGG 5 cut(s) 46, 130, 152, 374, 490
MvaI CCWGG 2 cut(s) 130, 490
MwoI GCNNNNNNNGC 1 cut(s) 187
NaeI GCCGGC 1 cut(s) 428
NciI CCSGG 3 cut(s) 46, 152, 374
NdeII GATC 2 cut(s) 9, 73
NgoMIV GCCGGC 1 cut(s) 426
NlaIII CATG 1 cut(s) 185
NsiI ATGCAT 1 cut(s) 183
PaeR7I CTCGAG 1 cut(s) 435
PdiI GCCGGC 1 cut(s) 428
PfeI GAWTC 1 cut(s) 389
PflMI CCANNNNNTGG 1 cut(s) 83
PkrI GCNGC 2 cut(s) 91, 94
Ple19I CGATCG 1 cut(s) 12
PshBI ATTAAT 1 cut(s) 230
Psp6I CCWGG 2 cut(s) 128, 488
PspGI CCWGG 2 cut(s) 128, 488
PspPI GGNCC 3 cut(s) 132, 307, 371
PspXI VCTCGAGB 1 cut(s) 435
PvuI CGATCG 1 cut(s) 12
RigI GGCCGGCC 1 cut(s) 430
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 2 cut(s) 90, 93
Sau3AI GATC 2 cut(s) 9, 73
Sau96I GGNCC 3 cut(s) 132, 307, 371
ScrFI CCNGG 5 cut(s) 46, 130, 152, 374, 490
SfaNI GCATC 3 cut(s) 29, 168, 386
Sfr274I CTCGAG 1 cut(s) 435
SinI GGWCC 1 cut(s) 132
SlaI CTCGAG 1 cut(s) 435
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
Sse9I AATT 1 cut(s) 557
SsiI CCGC 3 cut(s) 89, 92, 95
StyD4I CCNGG 5 cut(s) 44, 128, 150, 372, 488
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 550
TaqI TCGA 6 cut(s) 72, 220, 330, 408, 436, 483
TasI AATT 1 cut(s) 557
TauI GCSGC 2 cut(s) 92, 95
TfiI GAWTC 1 cut(s) 389
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TscAI CASTG 1 cut(s) 509
TspDTI ATGAA 1 cut(s) 569
TspRI CASTG 1 cut(s) 509
Van91I CCANNNNNTGG 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 132
VspI ATTAAT 1 cut(s) 230
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
XhoI CTCGAG 1 cut(s) 435
Zsp2I ATGCAT 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.