RLG00000027115

U4 U6 small nuclear ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001559
Physical Location & Seq
Reverse (-)
8538923 .. 8543435
4513 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027115

Sequence Viewer

Length: 519 bp
ATGGCAATTGATAGAGACGAAAACCCTATCGATGTTACTCAGTGCTATCGTGTCAATGATGCTTCACTCCACCGAGAGGTAGATAGTGTTGAGGATTGCGCAATGGGTCTTTTATTTGCATTTCAGGATTTCTGGCAACATTACTGGCAACCGATTTGCGGGGTTTCTTTGTTGGGTGTTTTTTGGATTTACAGTTCGTCAAATTTGATTGGTGTTGAAAGAGGGTTTCTGGGTTATTGGCTTAACCTTGAGGTGGAGATTGCTTCTACGTTCAAGGTTTCACATGCTATTGTTGTAAGAAATTGGTCTGCAAGGTCTGAAGAGAAGGAGAAGGTTGAATCTGTTGAAGGTGATGCTGCTGTTGCAAACATGGAGCAAGATGTCAATGGGGACTTGGCAAACTTAAAGACACTTAACTATGATGATCTGGATAGTGTTTCAAAAATGCAAAAATCACAAAGATTTGCCGATATAATGCGGATGTTTTGGTCTCCAAAAATTTTAAGTCTATATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Nop Prp31_C

Protein Analysis

173

Amino Acids

19.73

Weight (kDa)

4.6

Isoelectric Point (pI)

28.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 100
AciI CCGC 2 cut(s) 159, 478
AcsI RAATTY 2 cut(s) 202, 498
AcuI CTGAAG 1 cut(s) 339
AfiI CCNNNNNNNGG 3 cut(s) 76, 158, 253
AgsI TTSAA 5 cut(s) 218, 274, 338, 347, 441
AloI GAACNNNNNNTCC 2 cut(s) 178, 210
Alw26I GTCTC 2 cut(s) 9, 495
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 2 cut(s) 202, 498
AspLEI GCGC 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 362
BbvI GCAGC 1 cut(s) 343
BcoDI GTCTC 2 cut(s) 9, 495
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
BmsI GCATC 2 cut(s) 49, 343
BpuEI CTTGAG 1 cut(s) 269
Bsa29I ATCGAT 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 495
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 158, 253
Bse1I ACTGG 1 cut(s) 149
Bse3DI GCAATG 1 cut(s) 108
BseCI ATCGAT 1 cut(s) 30
BseGI GGATG 1 cut(s) 486
BseLI CCNNNNNNNGG 3 cut(s) 76, 158, 253
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 1 cut(s) 53
BseNI ACTGG 1 cut(s) 149
BseXI GCAGC 1 cut(s) 343
BshVI ATCGAT 1 cut(s) 30
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 3 cut(s) 76, 158, 253
BsmAI GTCTC 2 cut(s) 9, 495
BsmBI CGTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 404
Bso31I GGTCTC 1 cut(s) 495
Bsp143I GATC 1 cut(s) 424
BspACI CCGC 2 cut(s) 159, 478
BspCNI CTCAG 1 cut(s) 52
BspDI ATCGAT 1 cut(s) 30
BspTNI GGTCTC 1 cut(s) 495
BsrDI GCAATG 1 cut(s) 108
BsrI ACTGG 1 cut(s) 149
BssMI GATC 1 cut(s) 424
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 1 cut(s) 315
BstDEI CTNAG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 486
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 1 cut(s) 427
BstMAI GTCTC 2 cut(s) 9, 495
BstMBI GATC 1 cut(s) 424
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNSI RCATGY 1 cut(s) 287
BstV1I GCAGC 1 cut(s) 343
Bsu15I ATCGAT 1 cut(s) 30
BsuTUI ATCGAT 1 cut(s) 30
BtsCI GGATG 1 cut(s) 486
BtsIMutI CAGTG 1 cut(s) 47
CfoI GCGC 1 cut(s) 101
ClaI ATCGAT 1 cut(s) 30
CviAII CATG 2 cut(s) 284, 370
CviJI RGCY 1 cut(s) 241
CviKI_1 RGCY 1 cut(s) 241
DdeI CTNAG 1 cut(s) 39
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 495
Eco57I CTGAAG 1 cut(s) 339
Esp3I CGTCTC 1 cut(s) 9
FaeI CATG 2 cut(s) 287, 373
FaiI YATR 6 cut(s) 285, 371, 420, 473, 511, 513
FaqI GGGAC 1 cut(s) 404
FatI CATG 2 cut(s) 283, 369
FauI CCCGC 1 cut(s) 152
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 1 cut(s) 493
Fsp4HI GCNGC 1 cut(s) 357
FspI TGCGCA 1 cut(s) 100
GlaI GCGC 1 cut(s) 100
GluI GCNGC 1 cut(s) 357
HhaI GCGC 1 cut(s) 101
Hin1II CATG 2 cut(s) 287, 373
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HinfI GANTC 1 cut(s) 338
HphI GGTGA 1 cut(s) 362
Hpy188I TCNGA 2 cut(s) 319, 518
Hpy188III TCNNGA 2 cut(s) 125, 428
HpyAV CCTTC 3 cut(s) 319, 325, 341
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 4 cut(s) 119, 311, 365, 448
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 1 cut(s) 39
HpySE526I ACGT 1 cut(s) 269
Hsp92II CATG 2 cut(s) 287, 373
HspAI GCGC 1 cut(s) 99
Kzo9I GATC 1 cut(s) 424
LmnI GCTCC 1 cut(s) 373
LpnPI CCDG 5 cut(s) 110, 118, 130, 215, 413
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 2 cut(s) 49, 343
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 1 cut(s) 34
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 1 cut(s) 332
MfeI CAATTG 1 cut(s) 6
MluCI AATT 4 cut(s) 6, 202, 301, 498
MnlI CCTC 4 cut(s) 70, 85, 215, 244
MseI TTAA 4 cut(s) 243, 404, 414, 503
MunI CAATTG 1 cut(s) 6
MwoI GCNNNNNNNGC 1 cut(s) 362
NdeII GATC 1 cut(s) 424
NlaIII CATG 2 cut(s) 287, 373
NsbI TGCGCA 1 cut(s) 100
NspI RCATGY 1 cut(s) 287
PfeI GAWTC 1 cut(s) 338
PkrI GCNGC 1 cut(s) 358
SaqAI TTAA 4 cut(s) 243, 404, 414, 503
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 1 cut(s) 424
SetI ASST 8 cut(s) 81, 249, 255, 272, 279, 317, 336, 352
SfaNI GCATC 2 cut(s) 49, 343
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 4 cut(s) 6, 202, 301, 498
SsiI CCGC 2 cut(s) 159, 478
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 1 cut(s) 272
TaqI TCGA 1 cut(s) 30
TasI AATT 4 cut(s) 6, 202, 301, 498
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 4 cut(s) 243, 404, 414, 503
Tru9I TTAA 4 cut(s) 243, 404, 414, 503
TscAI CASTG 1 cut(s) 47
TseI GCWGC 1 cut(s) 356
TspRI CASTG 1 cut(s) 47
XapI RAATTY 2 cut(s) 202, 498
XceI RCATGY 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.