FvH4_1g21750

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001035
Physical Location & Seq
Reverse (-)
13701355 .. 13706188
4834 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21750.t4

Sequence Viewer

Length: 1044 bp
ATGTCGAATTTCATGGCGGCGCCCAATCAGAACACCAACCCCAACAAGTCCTTCGAGGTGGGTGAAGCTCCAAACGACTCCATTTCGAGCCTCAGCTTCAGCCCCAAAGCCAACCACCTCATCGCCACCTCCTGGGACAACCAGGTTCGATGCTGGGAGATACATCACAGTGGACAAAATCTTCATAGTGCCCCCAAGGGAATGATCTCGCATGACCATCCGGCTTTGTGCTCGGCTTGGAAGGATGATGGGGCAACTGTCTTCTCTGGAGGCTGTGACAAGCAAGCTAAGATGTGGTCCTTAGCTACTAACCAAACAATGACAGTTGCTGCTCATGAGGGCCCCATTAAAGAGATGGCTTGGATCTCCGAGTTGCAGCTCTTAGTCACCGGAAGCTTGGACAAGACTCTAAAGTATTGGGATACCAGGCAACAAAACCCAGTGCACACCCAACAACTTCCAGAACGCTGCTATGCGCTATCAGTGAACAAGGATCTATTGGTTGTTGGGACGGCAGATAGAAATATTATTATTTTTAAACTGCAGAACCCCGGGAATGAGTTCAAGAGAATTGTTTCACCCCTAAAGTTTCAGACAAGGTGTGTTGCTGCATTTCCTGATCAGCAAGGATTCTTAGTCGGATCTATTGAGGGGAGGGTTGGTGTGCATCATCTGGAGGAGCACCAATCAACTAAAAACTTCACCTTCAAATGCCACAGGGAGGGCAATGAGATATACTCTGTCAACTCTCTGAATTTCCATCCTGTGCACCACACATTTGCTACTGCTGGCTCTGATGGTTCTTTCAATTTTTGGGACAAGGACAGCAAACAAAGACTTAAGGCAATGCAAAGGTGCAGTCAGCCTATACCTTGCAGTACATTCAATCAAGATGGTTCCATATATGCATATGCGGTTTGCTATGATTGGAGCAAGGGTGCAGAAAATCATAATCCTACTACAGCAAAGAATCACATTTTTCTCCATTTGCCACAGGAATCTGAGGTGAAAGCCAAGCCGCGAGTTTCAACTGGAAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

348

Amino Acids

38.86

Weight (kDa)

8.32

Isoelectric Point (pI)

34.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 21 - 151 1e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 22 - 181 2.7e-20 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 23 - 177 3.9e-11 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 25 - 235 3.1e-22 WDR5 beta-propeller domain
WDR55 PF24796 25 - 233 6e-18 WDR55
Beta-prop_WDR3_2nd PF25172 26 - 180 3.3e-11 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 27 - 185 1.3e-07 Aladin seven-bladed propeller
WD40_Gbeta PF25391 33 - 145 2.7e-07 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 72 - 153 6.6e-09 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 76 - 305 1.6e-30 THOC3 beta-propeller domain
EIF3I PF24805 80 - 283 1.1e-06 EIF3I
WD40_Prp19 PF24814 81 - 150 4.3e-08 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 124 - 236 2.1e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 152 - 286 7.9e-11 WDHD1 first WD40 domain
WD40_Prp19 PF24814 159 - 304 3.6e-13 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 161 - 284 4.2e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 196 - 301 8.9e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 19
AccB7I CCANNNNNTGG 1 cut(s) 132
AccII CGCG 1 cut(s) 1021
AciI CCGC 3 cut(s) 17, 914, 1019
AclWI GGATC 3 cut(s) 371, 501, 649
AcsI RAATTY 2 cut(s) 7, 754
AcuI CTGAAG 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 20
AfaI GTAC 1 cut(s) 880
AfiI CCNNNNNNNGG 2 cut(s) 132, 721
AflII CTTAAG 1 cut(s) 839
AgsI TTSAA 5 cut(s) 565, 709, 808, 886, 1029
AjnI CCWGG 3 cut(s) 131, 141, 425
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
AluBI AGCT 6 cut(s) 68, 96, 287, 305, 379, 396
AluI AGCT 6 cut(s) 68, 96, 287, 305, 379, 396
Alw21I GWGCWC 4 cut(s) 233, 447, 684, 771
Alw44I GTGCAC 2 cut(s) 443, 767
AlwI GGATC 3 cut(s) 371, 501, 649
AlwNI CAGNNNCTG 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 1 cut(s) 340
ApaI GGGCCC 1 cut(s) 344
ApaLI GTGCAC 2 cut(s) 443, 767
ApeKI GCWGC 4 cut(s) 329, 376, 468, 608
ApoI RAATTY 2 cut(s) 7, 754
ArsI GACNNNNNNTTYG 2 cut(s) 844, 876
Asp700I GAANNNNTTC 2 cut(s) 560, 574
AspLEI GCGC 2 cut(s) 22, 478
AspS9I GGNCC 3 cut(s) 297, 340, 341
AsuC2I CCSGG 2 cut(s) 552, 553
AsuHPI GGTGA 5 cut(s) 74, 379, 570, 694, 1018
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 1 cut(s) 297
BaeGI GKGCMC 4 cut(s) 193, 344, 447, 771
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 253
Bbv12I GWGCWC 4 cut(s) 233, 447, 684, 771
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 4 cut(s) 316, 388, 455, 595
BccI CCATC 6 cut(s) 225, 242, 349, 768, 791, 887
BceAI ACGGC 1 cut(s) 528
BciT130I CCWGG 3 cut(s) 133, 143, 427
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 1 cut(s) 619
BcnI CCSGG 2 cut(s) 552, 553
BfmI CTRYAG 2 cut(s) 542, 960
BfoI RGCGCY 1 cut(s) 23
BfrI CTTAAG 1 cut(s) 839
BfuI GTATCC 1 cut(s) 415
BisI GCNGC 6 cut(s) 18, 330, 377, 469, 609, 1019
BlsI GCNGC 6 cut(s) 19, 331, 378, 470, 610, 1020
Bme1390I CCNGG 5 cut(s) 133, 143, 427, 552, 553
Bme18I GGWCC 1 cut(s) 297
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 3 cut(s) 297, 340, 341
BmiI GGNNCC 4 cut(s) 21, 342, 343, 898
BmrFI CCNGG 5 cut(s) 133, 143, 427, 552, 553
BmrI ACTGGG 1 cut(s) 434
BmsI GCATC 2 cut(s) 140, 676
BmuI ACTGGG 1 cut(s) 434
BpiI GAAGAC 1 cut(s) 253
BplI GAGNNNNNCTC 2 cut(s) 722, 754
BpmI CTGGAG 2 cut(s) 288, 695
Bpu10I CCTNAGC 2 cut(s) 92, 301
BpuMI CCSGG 2 cut(s) 552, 553
BsaHI GRCGYC 1 cut(s) 20
BsaJI CCNNGG 4 cut(s) 132, 195, 550, 551
BsaWI WCCGGW 1 cut(s) 389
BsaXI ACNNNNNCTCC 4 cut(s) 646, 676, 922, 952
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 721
Bse1I ACTGG 2 cut(s) 440, 1036
Bse3DI GCAATG 2 cut(s) 733, 852
BseBI CCWGG 3 cut(s) 133, 143, 427
BseDI CCNNGG 4 cut(s) 132, 195, 550, 551
BseGI GGATG 3 cut(s) 217, 250, 760
BseLI CCNNNNNNNGG 2 cut(s) 132, 721
BseMI GCAATG 2 cut(s) 733, 852
BseMII CTCAG 2 cut(s) 106, 993
BseNI ACTGG 2 cut(s) 440, 1036
BseRI GAGGAG 1 cut(s) 692
BseSI GKGCMC 4 cut(s) 193, 344, 447, 771
BseXI GCAGC 4 cut(s) 316, 388, 455, 595
BseYI CCCAGC 1 cut(s) 153
BsgI GTGCAG 2 cut(s) 877, 960
Bsh1236I CGCG 1 cut(s) 1021
BshFI GGCC 1 cut(s) 342
BshNI GGYRCC 1 cut(s) 19
BsiHKAI GWGCWC 4 cut(s) 233, 447, 684, 771
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 3 cut(s) 221, 390, 552
BslFI GGGAC 3 cut(s) 149, 523, 830
BslI CCNNNNNNNGG 2 cut(s) 132, 721
BsmFI GGGAC 3 cut(s) 149, 523, 830
BsnI GGCC 1 cut(s) 342
BsoBI CYCGRG 1 cut(s) 551
Bsp120I GGGCCC 1 cut(s) 340
Bsp1286I GDGCHC 6 cut(s) 193, 233, 344, 447, 684, 771
Bsp143I GATC 5 cut(s) 204, 363, 493, 619, 641
BspACI CCGC 3 cut(s) 17, 914, 1019
BspANI GGCC 1 cut(s) 342
BspCNI CTCAG 2 cut(s) 105, 994
BspFNI CGCG 1 cut(s) 1021
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 4 cut(s) 21, 342, 343, 898
BspMAI CTGCAG 1 cut(s) 546
BspPI GGATC 3 cut(s) 371, 501, 649
BspT107I GGYRCC 1 cut(s) 19
BspTI CTTAAG 1 cut(s) 839
BsrDI GCAATG 2 cut(s) 733, 852
BsrI ACTGG 2 cut(s) 440, 1036
BssECI CCNNGG 4 cut(s) 132, 195, 550, 551
BssMI GATC 5 cut(s) 204, 363, 493, 619, 641
BssNI GRCGYC 1 cut(s) 20
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 3 cut(s) 133, 143, 427
Bst4CI ACNGT 3 cut(s) 170, 259, 325
BstACI GRCGYC 1 cut(s) 20
BstAFI CTTAAG 1 cut(s) 839
BstC8I GCNNGC 2 cut(s) 285, 790
BstDEI CTNAG 6 cut(s) 92, 288, 301, 382, 634, 1002
BstF5I GGATG 3 cut(s) 217, 250, 760
BstFNI CGCG 1 cut(s) 1021
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 2 cut(s) 22, 478
BstKTI GATC 5 cut(s) 207, 366, 496, 622, 644
BstMBI GATC 5 cut(s) 204, 363, 493, 619, 641
BstNI CCWGG 3 cut(s) 133, 143, 427
BstSCI CCNGG 5 cut(s) 131, 141, 425, 550, 551
BstSFI CTRYAG 2 cut(s) 542, 960
BstSLI GKGCMC 4 cut(s) 193, 344, 447, 771
BstUI CGCG 1 cut(s) 1021
BstV1I GCAGC 4 cut(s) 316, 388, 455, 595
BstV2I GAAGAC 1 cut(s) 253
BstX2I RGATCY 3 cut(s) 363, 493, 641
BstYI RGATCY 3 cut(s) 363, 493, 641
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 1 cut(s) 342
BtgZI GCGATG 1 cut(s) 106
BtsCI GGATG 3 cut(s) 217, 250, 760
BtsIMutI CAGTG 3 cut(s) 175, 447, 489
Cac8I GCNNGC 2 cut(s) 285, 790
CaiI CAGNNNCTG 1 cut(s) 329
CciI TCATGA 1 cut(s) 334
CfoI GCGC 2 cut(s) 22, 478
Cfr13I GGNCC 3 cut(s) 297, 340, 341
Cfr9I CCCGGG 1 cut(s) 551
CsiI ACCWGGT 1 cut(s) 141
Csp6I GTAC 1 cut(s) 879
CviAII CATG 3 cut(s) 13, 212, 335
CviQI GTAC 1 cut(s) 879
DdeI CTNAG 6 cut(s) 92, 288, 301, 382, 634, 1002
DinI GGCGCC 1 cut(s) 21
DpnI GATC 5 cut(s) 206, 365, 495, 621, 643
DpnII GATC 5 cut(s) 204, 363, 493, 619, 641
DraI TTTAAA 1 cut(s) 538
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 344
Eco47I GGWCC 1 cut(s) 297
Eco57I CTGAAG 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 551
EcoO109I RGGNCCY 2 cut(s) 340, 341
EcoRII CCWGG 3 cut(s) 131, 141, 425
EcoT14I CCWWGG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 910
EcoT38I GRGCYC 1 cut(s) 344
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 3 cut(s) 16, 215, 338
FaqI GGGAC 3 cut(s) 149, 523, 830
FatI CATG 3 cut(s) 12, 211, 334
FauNDI CATATG 1 cut(s) 910
FbaI TGATCA 1 cut(s) 619
Fnu4HI GCNGC 6 cut(s) 18, 330, 377, 469, 609, 1019
FokI GGATG 3 cut(s) 204, 257, 747
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 6 cut(s) 18, 330, 377, 469, 609, 1019
GlaI GCGC 2 cut(s) 21, 477
GluI GCNGC 6 cut(s) 18, 330, 377, 469, 609, 1019
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 2 cut(s) 288, 695
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 1 cut(s) 342
HapII CCGG 3 cut(s) 221, 390, 552
HhaI GCGC 2 cut(s) 22, 478
Hin1I GRCGYC 1 cut(s) 20
Hin1II CATG 3 cut(s) 16, 215, 338
Hin6I GCGC 2 cut(s) 20, 476
HinP1I GCGC 2 cut(s) 20, 476
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 5 cut(s) 77, 406, 630, 970, 998
HpaII CCGG 3 cut(s) 221, 390, 552
HphI GGTGA 5 cut(s) 74, 379, 570, 694, 1018
Hpy166II GTNNAC 5 cut(s) 173, 445, 487, 745, 769
Hpy188I TCNGA 7 cut(s) 30, 370, 594, 641, 753, 796, 1003
Hpy188III TCNNGA 7 cut(s) 267, 335, 461, 565, 617, 674, 890
Hpy8I GTNNAC 5 cut(s) 173, 445, 487, 745, 769
HpyAV CCTTC 3 cut(s) 61, 235, 715
HpyCH4III ACNGT 3 cut(s) 170, 259, 325
HpyF3I CTNAG 6 cut(s) 92, 288, 301, 382, 634, 1002
Hsp92I GRCGYC 1 cut(s) 20
Hsp92II CATG 3 cut(s) 16, 215, 338
HspAI GCGC 2 cut(s) 20, 476
KasI GGCGCC 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 5 cut(s) 204, 363, 493, 619, 641
LmnI GCTCC 3 cut(s) 73, 679, 930
Lsp1109I GCAGC 4 cut(s) 316, 388, 455, 595
LweI GCATC 2 cut(s) 140, 676
MabI ACCWGGT 1 cut(s) 141
MaeIII GTNAC 2 cut(s) 275, 385
MalI GATC 5 cut(s) 206, 365, 495, 621, 643
MboI GATC 5 cut(s) 204, 363, 493, 619, 641
MboII GAAGA 2 cut(s) 173, 253
MflI RGATCY 3 cut(s) 363, 493, 641
MhlI GDGCHC 6 cut(s) 193, 233, 344, 447, 684, 771
MluCI AATT 4 cut(s) 7, 570, 754, 808
Mly113I GGCGCC 1 cut(s) 20
MlyI GAGTC 2 cut(s) 71, 400
MmeI TCCRAC 1 cut(s) 619
Mph1103I ATGCAT 1 cut(s) 910
MroXI GAANNNNTTC 2 cut(s) 560, 574
MseI TTAA 3 cut(s) 348, 537, 840
MslI CAYNNNNRTG 1 cut(s) 168
MspCI CTTAAG 1 cut(s) 839
MspI CCGG 3 cut(s) 221, 390, 552
MspR9I CCNGG 5 cut(s) 133, 143, 427, 552, 553
MvaI CCWGG 3 cut(s) 133, 143, 427
MvnI CGCG 1 cut(s) 1021
NarI GGCGCC 1 cut(s) 20
NciI CCSGG 2 cut(s) 552, 553
NdeI CATATG 1 cut(s) 910
NdeII GATC 5 cut(s) 204, 363, 493, 619, 641
NlaIII CATG 3 cut(s) 16, 215, 338
NlaIV GGNNCC 4 cut(s) 21, 342, 343, 898
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 2 cut(s) 275, 385
NsiI ATGCAT 1 cut(s) 910
PagI TCATGA 1 cut(s) 334
PdmI GAANNNNTTC 2 cut(s) 560, 574
PfeI GAWTC 3 cut(s) 630, 970, 998
PflMI CCANNNNNTGG 1 cut(s) 132
PkrI GCNGC 6 cut(s) 19, 331, 378, 470, 610, 1020
PleI GAGTC 2 cut(s) 71, 400
PluTI GGCGCC 1 cut(s) 23
PpsI GAGTC 2 cut(s) 71, 400
Psp6I CCWGG 3 cut(s) 131, 141, 425
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 3 cut(s) 131, 141, 425
PspN4I GGNNCC 4 cut(s) 21, 342, 343, 898
PspOMI GGGCCC 1 cut(s) 340
PspPI GGNCC 3 cut(s) 297, 340, 341
PstI CTGCAG 1 cut(s) 546
PstNI CAGNNNCTG 1 cut(s) 329
PsuI RGATCY 3 cut(s) 363, 493, 641
RsaI GTAC 1 cut(s) 880
RsaNI GTAC 1 cut(s) 879
RseI CAYNNNNRTG 1 cut(s) 168
SaqAI TTAA 3 cut(s) 348, 537, 840
SatI GCNGC 6 cut(s) 18, 330, 377, 469, 609, 1019
Sau3AI GATC 5 cut(s) 204, 363, 493, 619, 641
Sau96I GGNCC 3 cut(s) 297, 340, 341
SchI GAGTC 2 cut(s) 71, 400
ScrFI CCNGG 5 cut(s) 133, 143, 427, 552, 553
SduI GDGCHC 6 cut(s) 193, 233, 344, 447, 684, 771
SexAI ACCWGGT 1 cut(s) 141
SfaNI GCATC 2 cut(s) 140, 676
SfcI CTRYAG 2 cut(s) 542, 960
SfoI GGCGCC 1 cut(s) 21
SinI GGWCC 1 cut(s) 297
SmaI CCCGGG 1 cut(s) 553
SmiMI CAYNNNNRTG 1 cut(s) 168
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
Sse9I AATT 4 cut(s) 7, 570, 754, 808
SsiI CCGC 3 cut(s) 17, 914, 1019
SspDI GGCGCC 1 cut(s) 19
SspI AATATT 1 cut(s) 526
StyD4I CCNGG 5 cut(s) 131, 141, 425, 550, 551
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 3 cut(s) 170, 259, 325
TaqI TCGA 4 cut(s) 5, 54, 86, 148
TasI AATT 4 cut(s) 7, 570, 754, 808
TatI WGTACW 1 cut(s) 878
TauI GCSGC 2 cut(s) 20, 1021
TfiI GAWTC 3 cut(s) 630, 970, 998
Tru1I TTAA 3 cut(s) 348, 537, 840
Tru9I TTAA 3 cut(s) 348, 537, 840
TscAI CASTG 3 cut(s) 175, 447, 489
TseFI GTSAC 2 cut(s) 275, 385
TseI GCWGC 4 cut(s) 329, 376, 468, 608
Tsp45I GTSAC 2 cut(s) 275, 385
TspDTI ATGAA 1 cut(s) 173
TspMI CCCGGG 1 cut(s) 551
TspRI CASTG 3 cut(s) 175, 447, 489
Van91I CCANNNNNTGG 1 cut(s) 132
Vha464I CTTAAG 1 cut(s) 839
VneI GTGCAC 2 cut(s) 443, 767
VpaK11BI GGWCC 1 cut(s) 297
XapI RAATTY 2 cut(s) 7, 754
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XmaI CCCGGG 1 cut(s) 551
XmnI GAANNNNTTC 2 cut(s) 560, 574
Zsp2I ATGCAT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.