Rh2DG264200

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001035
Physical Location & Seq
Reverse (-)
28462640 .. 28466941
4302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG264200.1

Sequence Viewer

Length: 1044 bp
ATGTCGAGTTTCATGCCGGCCGCCAATCAGAACACGAATCCCAACAAGTCCTTCGAGGTCGCCGAACCTCCAAACGACTCCATTTCGAGCCTCAGCTTCAGCCCCAAAGCCAATCACCTCGTCGCCACCTCCTGGGACAACCAGGTTCGATGCTGGGAGATACAACACAGTGGACAAAATCTTGCTAGCACCCCCAAGGGAATGATTTCACACGACCATCCGGCTTTGTGCTCAACTTGGAAGGATGATGGGGCGACTGTCTTCTCTGGAGGATGTGACAAGCAAGCTAAGATGTGGTCCTTGGCTACTAACCAAACAATGACTGTTGCTGTGCATGATGCCCCCATTAAAGACATGGCTTGGATCCCCGAGTTGAACCTCCTAGTTACTGGAAGCTTGGACAAGACTGTAAAGTATTGGGATACGAGGCAGCAGAGCCCAGCGCACACCCAACAGCTCCCAGAACGCTGCTATGCACTATCAGTGAAACAGTCTCTAATGGTTGTTGGGACGGCGGATAGAAATATTATTATTTTTCACTTGCAGCAACCACAGAATGAGTTGAAGAGAATTGTTTCACCCCTAAAGTTTCAGACAAGGTGTGTTGCTGCATTCCCTGATCAGCAAGGATTCTTGGTTGGATCTATTGAGGGGAGGGTTGGTGTGCATCATCTGGATGAGCAACAATTATCTAAAAACTTTACCTTCAAATGCCACAGGGAGGGCAATGAGATATACTCTGTCAACTCTCTGAACTTCCATCCCGTCCACCACACGTTTGCTACCGCTGGCTCTGATGGTTCTTTTAATTTTTGGGACAAGGACAGCAAACAAAGACTCAAGGCAATGCAAAGGTGCAGTCAGCCTATACCTTGCAGTACCTTTAACCACGATGGTTCCATATATGCATATGCGGTTTGCTACGATTGGAGCAAGGGTGCAGAAAATCATAATCCTGCGGCAGCAAAGAACCACATTTTCCTCCATTTGCCACAGGAAGCTGAGGTAAAAGCCAAGCCACGAGTTTCAACTGGAAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

347

Amino Acids

38.77

Weight (kDa)

8.32

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 20 - 180 2.6e-19 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 22 - 176 1.7e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 25 - 236 1.1e-21 WDR5 beta-propeller domain
WDR55 PF24796 25 - 228 5.2e-17 WDR55
Beta-prop_WDR3_2nd PF25172 26 - 180 1.5e-09 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 27 - 186 1.1e-06 Aladin seven-bladed propeller
WD40_Gbeta PF25391 37 - 144 1.6e-07 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 72 - 153 4.7e-09 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 78 - 305 5e-31 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 79 - 150 1.2e-08 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 81 - 151 2.1e-09 Prp19 WD40 domain
WD40 PF00400 106 - 141 4.6e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 120 - 239 3.6e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 152 - 286 5.8e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 159 - 304 1.3e-13 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 161 - 284 1.6e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 168 - 300 6.1e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 132
AciI CCGC 5 cut(s) 21, 515, 786, 914, 959
AclWI GGATC 3 cut(s) 358, 371, 649
AcoI YGGCCR 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 82
AfaI GTAC 1 cut(s) 880
AfiI CCNNNNNNNGG 2 cut(s) 132, 721
AflIII ACRYGT 1 cut(s) 774
AgsI TTSAA 4 cut(s) 376, 565, 709, 1029
AjnI CCWGG 2 cut(s) 131, 141
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
AluBI AGCT 5 cut(s) 96, 287, 396, 457, 1001
AluI AGCT 5 cut(s) 96, 287, 396, 457, 1001
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 1 cut(s) 498
AlwI GGATC 3 cut(s) 358, 371, 649
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 5 cut(s) 430, 468, 544, 608, 962
ArsI GACNNNNNNTTYG 2 cut(s) 844, 876
Asp700I GAANNNNTTC 2 cut(s) 205, 574
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 1 cut(s) 297
AsuHPI GGTGA 2 cut(s) 107, 570
AsuNHI GCTAGC 1 cut(s) 185
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 1 cut(s) 297
BamHI GGATCC 1 cut(s) 363
BanII GRGCYC 1 cut(s) 440
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 233
BbvCI CCTCAGC 2 cut(s) 92, 1002
BbvI GCAGC 5 cut(s) 442, 455, 556, 595, 974
BccI CCATC 5 cut(s) 225, 242, 768, 791, 887
BceAI ACGGC 1 cut(s) 528
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 2 cut(s) 133, 143
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 1 cut(s) 498
BfaI CTAG 2 cut(s) 186, 383
BfuI GTATCC 1 cut(s) 415
BisI GCNGC 7 cut(s) 21, 431, 469, 545, 609, 960, 963
BlsI GCNGC 7 cut(s) 22, 432, 470, 546, 610, 961, 964
Bme1390I CCNGG 2 cut(s) 133, 143
Bme18I GGWCC 1 cut(s) 297
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 2 cut(s) 365, 898
BmrFI CCNGG 2 cut(s) 133, 143
BmsI GCATC 3 cut(s) 140, 328, 676
BmtI GCTAGC 1 cut(s) 189
BpiI GAAGAC 1 cut(s) 253
BplI GAGNNNNNCTC 2 cut(s) 722, 754
BpmI CTGGAG 1 cut(s) 288
Bpu10I CCTNAGC 2 cut(s) 92, 1002
BpuEI CTTGAG 1 cut(s) 824
BsaJI CCNNGG 3 cut(s) 132, 195, 300
BsaXI ACNNNNNCTCC 4 cut(s) 646, 676, 922, 952
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 721
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 2 cut(s) 394, 1036
Bse3DI GCAATG 2 cut(s) 733, 852
BseBI CCWGG 2 cut(s) 133, 143
BseDI CCNNGG 3 cut(s) 132, 195, 300
BseGI GGATG 5 cut(s) 217, 250, 278, 682, 760
BseLI CCNNNNNNNGG 2 cut(s) 132, 721
BseMI GCAATG 2 cut(s) 733, 852
BseMII CTCAG 2 cut(s) 106, 993
BseNI ACTGG 2 cut(s) 394, 1036
BseX3I CGGCCG 1 cut(s) 18
BseXI GCAGC 5 cut(s) 442, 455, 556, 595, 974
BseYI CCCAGC 2 cut(s) 153, 439
BsgI GTGCAG 2 cut(s) 877, 960
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 2 cut(s) 17, 221
BslFI GGGAC 3 cut(s) 149, 523, 830
BslI CCNNNNNNNGG 2 cut(s) 132, 721
BsmAI GTCTC 1 cut(s) 498
BsmFI GGGAC 3 cut(s) 149, 523, 830
BsmI GAATGC 1 cut(s) 611
BsnI GGCC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 233, 440
Bsp143I GATC 3 cut(s) 363, 619, 641
BspACI CCGC 5 cut(s) 21, 515, 786, 914, 959
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 2 cut(s) 105, 994
BspLI GGNNCC 2 cut(s) 365, 898
BspOI GCTAGC 1 cut(s) 189
BspPI GGATC 3 cut(s) 358, 371, 649
BsrDI GCAATG 2 cut(s) 733, 852
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 2 cut(s) 394, 1036
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 3 cut(s) 132, 195, 300
BssMI GATC 3 cut(s) 363, 619, 641
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 2 cut(s) 195, 300
Bst2BI CACGAG 1 cut(s) 1020
Bst2UI CCWGG 2 cut(s) 133, 143
Bst4CI ACNGT 5 cut(s) 170, 259, 325, 409, 492
Bst6I CTCTTC 1 cut(s) 560
BstC8I GCNNGC 4 cut(s) 18, 187, 285, 790
BstDEI CTNAG 3 cut(s) 92, 288, 1002
BstF5I GGATG 5 cut(s) 217, 250, 278, 682, 760
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 3 cut(s) 366, 622, 644
BstMAI GTCTC 1 cut(s) 498
BstMBI GATC 3 cut(s) 363, 619, 641
BstMCI CGRYCG 1 cut(s) 21
BstNI CCWGG 2 cut(s) 133, 143
BstSCI CCNGG 2 cut(s) 131, 141
BstV1I GCAGC 5 cut(s) 442, 455, 556, 595, 974
BstV2I GAAGAC 1 cut(s) 253
BstX2I RGATCY 2 cut(s) 363, 641
BstYI RGATCY 2 cut(s) 363, 641
BstZI CGGCCG 1 cut(s) 18
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 1 cut(s) 20
BtsCI GGATG 5 cut(s) 217, 250, 278, 682, 760
BtsIMutI CAGTG 2 cut(s) 175, 489
Cac8I GCNNGC 4 cut(s) 18, 187, 285, 790
CfoI GCGC 1 cut(s) 445
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 297
CsiI ACCWGGT 1 cut(s) 141
Csp6I GTAC 1 cut(s) 879
CviAII CATG 3 cut(s) 13, 335, 355
CviQI GTAC 1 cut(s) 879
DdeI CTNAG 3 cut(s) 92, 288, 1002
DpnI GATC 3 cut(s) 365, 621, 643
DpnII GATC 3 cut(s) 363, 619, 641
EaeI YGGCCR 1 cut(s) 18
EagI CGGCCG 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 560
EarI CTCTTC 1 cut(s) 560
EciI GGCGGA 1 cut(s) 530
EclXI CGGCCG 1 cut(s) 18
Eco130I CCWWGG 2 cut(s) 195, 300
Eco24I GRGCYC 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 297
Eco52I CGGCCG 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 131, 141
EcoT14I CCWWGG 2 cut(s) 195, 300
EcoT22I ATGCAT 1 cut(s) 910
EcoT38I GRGCYC 1 cut(s) 440
ErhI CCWWGG 2 cut(s) 195, 300
FaeI CATG 3 cut(s) 16, 338, 358
FaqI GGGAC 3 cut(s) 149, 523, 830
FatI CATG 3 cut(s) 12, 334, 354
FauNDI CATATG 1 cut(s) 910
FbaI TGATCA 1 cut(s) 619
Fnu4HI GCNGC 7 cut(s) 21, 431, 469, 545, 609, 960, 963
FokI GGATG 5 cut(s) 204, 257, 285, 689, 747
FriOI GRGCYC 1 cut(s) 440
Fsp4HI GCNGC 7 cut(s) 21, 431, 469, 545, 609, 960, 963
FspBI CTAG 2 cut(s) 186, 383
GlaI GCGC 1 cut(s) 444
GluI GCNGC 7 cut(s) 21, 431, 469, 545, 609, 960, 963
GsaI CCCAGC 2 cut(s) 157, 443
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 1 cut(s) 20
HapII CCGG 2 cut(s) 17, 221
HhaI GCGC 1 cut(s) 445
Hin1II CATG 3 cut(s) 16, 338, 358
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 4 cut(s) 37, 77, 630, 837
HpaII CCGG 2 cut(s) 17, 221
HphI GGTGA 2 cut(s) 107, 570
Hpy166II GTNNAC 3 cut(s) 173, 745, 769
Hpy188I TCNGA 4 cut(s) 30, 594, 753, 796
Hpy188III TCNNGA 2 cut(s) 267, 674
Hpy8I GTNNAC 3 cut(s) 173, 745, 769
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 3 cut(s) 61, 235, 715
HpyCH4III ACNGT 5 cut(s) 170, 259, 325, 409, 492
HpyCH4IV ACGT 1 cut(s) 776
HpyF3I CTNAG 3 cut(s) 92, 288, 1002
HpySE526I ACGT 1 cut(s) 776
Hsp92II CATG 3 cut(s) 16, 338, 358
HspAI GCGC 1 cut(s) 443
KroI GCCGGC 1 cut(s) 16
KroNI GCCGGC 1 cut(s) 18
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 3 cut(s) 363, 619, 641
LmnI GCTCC 2 cut(s) 462, 930
Lsp1109I GCAGC 5 cut(s) 442, 455, 556, 595, 974
LweI GCATC 3 cut(s) 140, 328, 676
MabI ACCWGGT 1 cut(s) 141
MaeI CTAG 2 cut(s) 186, 383
MaeII ACGT 1 cut(s) 776
MaeIII GTNAC 2 cut(s) 275, 385
MalI GATC 3 cut(s) 365, 621, 643
MboI GATC 3 cut(s) 363, 619, 641
MboII GAAGA 2 cut(s) 253, 577
MflI RGATCY 2 cut(s) 363, 641
MhlI GDGCHC 2 cut(s) 233, 440
MluCI AATT 3 cut(s) 570, 686, 808
MlyI GAGTC 2 cut(s) 71, 831
MmeI TCCRAC 1 cut(s) 619
Mph1103I ATGCAT 1 cut(s) 910
MroNI GCCGGC 1 cut(s) 16
MroXI GAANNNNTTC 2 cut(s) 205, 574
MseI TTAA 3 cut(s) 348, 807, 885
MslI CAYNNNNRTG 1 cut(s) 675
MspA1I CMGCKG 1 cut(s) 788
MspI CCGG 2 cut(s) 17, 221
MspR9I CCNGG 2 cut(s) 133, 143
Mva1269I GAATGC 1 cut(s) 611
MvaI CCWGG 2 cut(s) 133, 143
NaeI GCCGGC 1 cut(s) 18
NdeI CATATG 1 cut(s) 910
NdeII GATC 3 cut(s) 363, 619, 641
NgoMIV GCCGGC 1 cut(s) 16
NheI GCTAGC 1 cut(s) 185
NlaIII CATG 3 cut(s) 16, 338, 358
NlaIV GGNNCC 2 cut(s) 365, 898
NmuCI GTSAC 1 cut(s) 275
NsiI ATGCAT 1 cut(s) 910
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 1 cut(s) 611
PdiI GCCGGC 1 cut(s) 18
PdmI GAANNNNTTC 2 cut(s) 205, 574
PfeI GAWTC 2 cut(s) 37, 630
PflMI CCANNNNNTGG 1 cut(s) 132
PkrI GCNGC 7 cut(s) 22, 432, 470, 546, 610, 961, 964
PleI GAGTC 2 cut(s) 71, 831
PpsI GAGTC 2 cut(s) 71, 831
Psp6I CCWGG 2 cut(s) 131, 141
PspFI CCCAGC 2 cut(s) 153, 439
PspGI CCWGG 2 cut(s) 131, 141
PspN4I GGNNCC 2 cut(s) 365, 898
PspPI GGNCC 1 cut(s) 297
PsuI RGATCY 2 cut(s) 363, 641
RsaI GTAC 1 cut(s) 880
RsaNI GTAC 1 cut(s) 879
RseI CAYNNNNRTG 1 cut(s) 675
SaqAI TTAA 3 cut(s) 348, 807, 885
SatI GCNGC 7 cut(s) 21, 431, 469, 545, 609, 960, 963
Sau3AI GATC 3 cut(s) 363, 619, 641
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 2 cut(s) 71, 831
ScrFI CCNGG 2 cut(s) 133, 143
SduI GDGCHC 2 cut(s) 233, 440
SexAI ACCWGGT 1 cut(s) 141
SfaNI GCATC 3 cut(s) 140, 328, 676
SinI GGWCC 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
Sse9I AATT 3 cut(s) 570, 686, 808
SsiI CCGC 5 cut(s) 21, 515, 786, 914, 959
SspI AATATT 1 cut(s) 526
SspMI CTAG 2 cut(s) 186, 383
StyD4I CCNGG 2 cut(s) 131, 141
StyI CCWWGG 2 cut(s) 195, 300
TaaI ACNGT 5 cut(s) 170, 259, 325, 409, 492
TaiI ACGT 1 cut(s) 779
TaqI TCGA 4 cut(s) 5, 54, 86, 148
TasI AATT 3 cut(s) 570, 686, 808
TauI GCSGC 2 cut(s) 23, 962
TfiI GAWTC 2 cut(s) 37, 630
Tru1I TTAA 3 cut(s) 348, 807, 885
Tru9I TTAA 3 cut(s) 348, 807, 885
TscAI CASTG 2 cut(s) 175, 489
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 5 cut(s) 430, 468, 544, 608, 962
Tsp45I GTSAC 1 cut(s) 275
TspRI CASTG 2 cut(s) 175, 489
Van91I CCANNNNNTGG 1 cut(s) 132
VpaK11BI GGWCC 1 cut(s) 297
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XmnI GAANNNNTTC 2 cut(s) 205, 574
XspI CTAG 2 cut(s) 186, 383
Zsp2I ATGCAT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.