Rroxscaffold_3G00266760

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001035
Physical Location & Seq
Forward (+)
59957175 .. 59962676
5502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00266760.1

Sequence Viewer

Length: 1029 bp
ATGTCGGCTTTCATGAATCACAATCCGAACAATTCATTTGAGGTCGCTCAGCCTCCAAGCGATTCTGTTTCAAGCCTCAGTTTCAGTCCCAAATCGAACTTTCTGGTCTCTACCTCCTGGGACAATCAGGTGCGATGCTGGGATATTCAAAGAATTGGAACAAATATTGCTGCTGTGCCGAAGAGCTCCTTTTCGCATGACCATCTGGTTTTGTGCTCAACATGGAAGGATGATGGGATGGCTGTATTCTCTGGAGGTTGTGACAGGCAAGTTAAGATGTGGTCTCTAATGTCTGGTGGCCAAACGAGCACTGTTTCCATGCATGATGCACCCATAAAAGAGATTGCTTGGATTCCCGAGATGGCGCTCTTAGTCACAGGAAGCTGGGACAAGACTATGAAGTACTGGGATTTGAGGCAGCCAAATCCAGTGCATACCCAACAACTACCCGAACGCTGTTATGCACTAACAGTGAAACACCCTCTAATGGTTGTTGGAACAGCAGATAGAAATCTGATCATATTTAACTTGCAGAGTCCACAGACTGAGTTCAAGAGAATCATTTCACCCCTGAAGTATCAGACAAGGTGTGTTGCTGCATTTCCTGATAAGCAAGGTTTCTTGGTTGGTTCAATTGAAGGGAGGGTTGGAGTGCAGCATCTGGATGAAGGACAAGATAGTAAAAACTTTACCTTCAAATGCCACAGAGAAAACAATGCTCTTTACTCAGTCAACTCTATAAACTTCCATCCTGTGCATCACACATTTTCTACAGCTGGTTCTGATGGTTCTATCAGTTTTTGGGATAAGGACAGCAAACAGAGACTCAAGGCCATGCAAAGGTGCAATCAACCCATTCCTTGCAGTACTTTCAACAATGATGGTTCCATATATGCATACTCGGTTTGCTATGACTGGCATAAGGGAGCAGAAAATCATAATCCTTCTACTGCAAAGACCTCCATTTTCCTCCACTTGCCACAGGACGCTGATGTTAAAAGCAAGCCAAGACCTGGTGGAAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

342

Amino Acids

38.43

Weight (kDa)

8.67

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 11 - 146 3.8e-13 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 12 - 154 1.6e-15 Prp19 WD40 domain
Beta-prop_EML PF23409 18 - 168 6.1e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 20 - 48 1.4e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 21 - 178 7.4e-21 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 22 - 139 4.5e-09 TEP-1 second beta-propeller
WD40_Gbeta PF25391 33 - 140 1.4e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 65 - 176 2.7e-15 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 65 - 141 8.6e-08 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 66 - 301 1.2e-29 THOC3 beta-propeller domain
WDR55 PF24796 66 - 223 2.3e-11 WDR55
WD40_WDHD1_1st PF24817 70 - 149 6.2e-10 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 116 - 224 2.3e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 148 - 284 1.7e-09 WDHD1 first WD40 domain
WD40_Prp19 PF24814 159 - 300 7e-12 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 165 - 297 1.3e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1013
AcoI YGGCCR 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 593
AfaI GTAC 2 cut(s) 404, 868
AfiI CCNNNNNNNGG 3 cut(s) 487, 840, 1013
AgsI TTSAA 7 cut(s) 72, 149, 553, 633, 638, 697, 874
AjnI CCWGG 2 cut(s) 116, 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 630, 662
AluBI AGCT 3 cut(s) 186, 384, 776
AluI AGCT 3 cut(s) 186, 384, 776
Alw21I GWGCWC 3 cut(s) 188, 218, 311
Alw26I GTCTC 3 cut(s) 112, 288, 817
AlwNI CAGNNNCTG 1 cut(s) 661
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 2 cut(s) 298, 831
ApeKI GCWGC 4 cut(s) 170, 418, 596, 655
Asp700I GAANNNNTTC 1 cut(s) 562
AspLEI GCGC 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 558
AvaI CYCGRG 1 cut(s) 356
BalI TGGCCA 1 cut(s) 300
BanII GRGCYC 1 cut(s) 188
Bbv12I GWGCWC 3 cut(s) 188, 218, 311
BbvI GCAGC 4 cut(s) 157, 430, 583, 667
BccI CCATC 7 cut(s) 210, 227, 232, 355, 756, 779, 875
BciT130I CCWGG 2 cut(s) 118, 1014
BclI TGATCA 1 cut(s) 516
BcoDI GTCTC 3 cut(s) 112, 288, 817
BfmI CTRYAG 1 cut(s) 771
BfoI RGCGCY 1 cut(s) 368
BisI GCNGC 4 cut(s) 171, 419, 597, 656
BlpI GCTNAGC 1 cut(s) 48
BlsI GCNGC 4 cut(s) 172, 420, 598, 657
BmcAI AGTACT 2 cut(s) 404, 868
Bme1390I CCNGG 2 cut(s) 118, 1014
BmeT110I CYCGRG 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 886
BmrFI CCNGG 2 cut(s) 118, 1014
BmrI ACTGGG 1 cut(s) 415
BmsI GCATC 4 cut(s) 125, 316, 667, 766
BmuI ACTGGG 1 cut(s) 415
BpmI CTGGAG 1 cut(s) 273
Bpu1102I GCTNAGC 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 812
BsaBI GATNNNNATC 1 cut(s) 510
BsaI GGTCTC 2 cut(s) 112, 288
BsaJI CCNNGG 1 cut(s) 117
Bsc4I CCNNNNNNNGG 3 cut(s) 487, 840, 1013
Bse1I ACTGG 3 cut(s) 410, 428, 920
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 2 cut(s) 118, 1014
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 4 cut(s) 235, 243, 670, 748
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 3 cut(s) 487, 840, 1013
BseMII CTCAG 4 cut(s) 62, 91, 537, 741
BseNI ACTGG 3 cut(s) 410, 428, 920
BseXI GCAGC 4 cut(s) 157, 430, 583, 667
BseYI CCCAGC 2 cut(s) 138, 384
BsgI GTGCAG 1 cut(s) 674
BshFI GGCC 2 cut(s) 300, 833
BsiHKAI GWGCWC 3 cut(s) 188, 218, 311
BsiHKCI CYCGRG 1 cut(s) 356
BslFI GGGAC 3 cut(s) 72, 134, 401
BslI CCNNNNNNNGG 3 cut(s) 487, 840, 1013
BsmAI GTCTC 3 cut(s) 112, 288, 817
BsmFI GGGAC 3 cut(s) 72, 134, 401
BsnI GGCC 2 cut(s) 300, 833
Bso31I GGTCTC 2 cut(s) 112, 288
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 3 cut(s) 188, 218, 311
Bsp143I GATC 1 cut(s) 516
Bsp1720I GCTNAGC 1 cut(s) 48
BspANI GGCC 2 cut(s) 300, 833
BspCNI CTCAG 4 cut(s) 61, 90, 538, 740
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 1 cut(s) 886
BspQI GCTCTTC 1 cut(s) 176
BspTNI GGTCTC 2 cut(s) 112, 288
BsrI ACTGG 3 cut(s) 410, 428, 920
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 1 cut(s) 516
Bst2UI CCWGG 2 cut(s) 118, 1014
Bst4CI ACNGT 2 cut(s) 313, 472
Bst6I CTCTTC 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 1004
BstDEI CTNAG 5 cut(s) 48, 77, 370, 546, 727
BstF5I GGATG 4 cut(s) 235, 243, 670, 748
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 1 cut(s) 519
BstMAI GTCTC 3 cut(s) 112, 288, 817
BstMBI GATC 1 cut(s) 516
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNI CCWGG 2 cut(s) 118, 1014
BstSCI CCNGG 2 cut(s) 116, 1012
BstSFI CTRYAG 1 cut(s) 771
BstV1I GCAGC 4 cut(s) 157, 430, 583, 667
BsuRI GGCC 2 cut(s) 300, 833
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 4 cut(s) 235, 243, 670, 748
BtsIMutI CAGTG 3 cut(s) 309, 435, 477
Cac8I GCNNGC 1 cut(s) 1004
CaiI CAGNNNCTG 1 cut(s) 661
CciI TCATGA 1 cut(s) 12
CfoI GCGC 1 cut(s) 367
CseI GACGC 1 cut(s) 995
CsiI ACCWGGT 1 cut(s) 1012
Csp6I GTAC 2 cut(s) 403, 867
CviAII CATG 6 cut(s) 13, 197, 222, 319, 323, 835
CviQI GTAC 2 cut(s) 403, 867
DdeI CTNAG 5 cut(s) 48, 77, 370, 546, 727
DpnI GATC 1 cut(s) 518
DpnII GATC 1 cut(s) 516
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 2 cut(s) 112, 288
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 116, 1012
EcoT22I ATGCAT 2 cut(s) 324, 898
EcoT38I GRGCYC 1 cut(s) 188
FaeI CATG 6 cut(s) 16, 200, 225, 322, 326, 838
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 3 cut(s) 72, 134, 401
FatI CATG 6 cut(s) 12, 196, 221, 318, 322, 834
FbaI TGATCA 1 cut(s) 516
Fnu4HI GCNGC 4 cut(s) 171, 419, 597, 656
FokI GGATG 4 cut(s) 242, 250, 677, 735
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 4 cut(s) 171, 419, 597, 656
GlaI GCGC 1 cut(s) 366
GluI GCNGC 4 cut(s) 171, 419, 597, 656
GsaI CCCAGC 2 cut(s) 142, 388
GsuI CTGGAG 1 cut(s) 273
HaeII RGCGCY 1 cut(s) 368
HaeIII GGCC 2 cut(s) 300, 833
HgaI GACGC 1 cut(s) 995
HhaI GCGC 1 cut(s) 367
Hin1II CATG 6 cut(s) 16, 200, 225, 322, 326, 838
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HinfI GANTC 6 cut(s) 16, 62, 352, 535, 558, 825
HphI GGTGA 1 cut(s) 558
Hpy166II GTNNAC 2 cut(s) 539, 733
Hpy188I TCNGA 4 cut(s) 27, 516, 582, 784
Hpy188III TCNNGA 6 cut(s) 13, 252, 356, 553, 605, 662
Hpy8I GTNNAC 2 cut(s) 539, 733
HpyAV CCTTC 6 cut(s) 220, 632, 662, 703, 954, 1017
HpyCH4III ACNGT 2 cut(s) 313, 472
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 5 cut(s) 48, 77, 370, 546, 727
Hsp92II CATG 6 cut(s) 16, 200, 225, 322, 326, 838
HspAI GCGC 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 516
Kzo9I GATC 1 cut(s) 516
LguI GCTCTTC 1 cut(s) 176
LmnI GCTCC 2 cut(s) 191, 926
Lsp1109I GCAGC 4 cut(s) 157, 430, 583, 667
LweI GCATC 4 cut(s) 125, 316, 667, 766
MabI ACCWGGT 1 cut(s) 1012
MaeIII GTNAC 2 cut(s) 260, 373
MalI GATC 1 cut(s) 518
MboI GATC 1 cut(s) 516
MboII GAAGA 1 cut(s) 193
MfeI CAATTG 1 cut(s) 633
MhlI GDGCHC 3 cut(s) 188, 218, 311
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 3 cut(s) 31, 153, 633
MluNI TGGCCA 1 cut(s) 300
MlyI GAGTC 2 cut(s) 544, 819
MmeI TCCRAC 2 cut(s) 475, 628
Mox20I TGGCCA 1 cut(s) 300
Mph1103I ATGCAT 2 cut(s) 324, 898
MroXI GAANNNNTTC 1 cut(s) 562
MscI TGGCCA 1 cut(s) 300
MseI TTAA 3 cut(s) 273, 525, 996
MslI CAYNNNNRTG 1 cut(s) 663
Msp20I TGGCCA 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 776
MspR9I CCNGG 2 cut(s) 118, 1014
MunI CAATTG 1 cut(s) 633
MvaI CCWGG 2 cut(s) 118, 1014
MwoI GCNNNNNNNGC 1 cut(s) 306
NdeII GATC 1 cut(s) 516
NlaIII CATG 6 cut(s) 16, 200, 225, 322, 326, 838
NlaIV GGNNCC 1 cut(s) 886
NmuCI GTSAC 2 cut(s) 260, 373
NsiI ATGCAT 2 cut(s) 324, 898
PagI TCATGA 1 cut(s) 12
PciSI GCTCTTC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 562
PfeI GAWTC 4 cut(s) 16, 62, 352, 558
PflMI CCANNNNNTGG 1 cut(s) 1013
PkrI GCNGC 4 cut(s) 172, 420, 598, 657
PleI GAGTC 2 cut(s) 543, 819
PpsI GAGTC 2 cut(s) 543, 819
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 116, 1012
PspFI CCCAGC 2 cut(s) 138, 384
PspGI CCWGG 2 cut(s) 116, 1012
PspN4I GGNNCC 1 cut(s) 886
PstNI CAGNNNCTG 1 cut(s) 661
PvuII CAGCTG 1 cut(s) 776
RsaI GTAC 2 cut(s) 404, 868
RsaNI GTAC 2 cut(s) 403, 867
RseI CAYNNNNRTG 1 cut(s) 663
SacI GAGCTC 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 176
SaqAI TTAA 3 cut(s) 273, 525, 996
SatI GCNGC 4 cut(s) 171, 419, 597, 656
Sau3AI GATC 1 cut(s) 516
ScaI AGTACT 2 cut(s) 404, 868
SchI GAGTC 2 cut(s) 544, 819
ScrFI CCNGG 2 cut(s) 118, 1014
SduI GDGCHC 3 cut(s) 188, 218, 311
SexAI ACCWGGT 1 cut(s) 1012
SfaNI GCATC 4 cut(s) 125, 316, 667, 766
SfcI CTRYAG 1 cut(s) 771
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
Sse9I AATT 3 cut(s) 31, 153, 633
SspI AATATT 1 cut(s) 166
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 116, 1012
TaaI ACNGT 2 cut(s) 313, 472
TaqI TCGA 1 cut(s) 95
TasI AATT 3 cut(s) 31, 153, 633
TatI WGTACW 2 cut(s) 402, 866
TfiI GAWTC 4 cut(s) 16, 62, 352, 558
Tru1I TTAA 3 cut(s) 273, 525, 996
Tru9I TTAA 3 cut(s) 273, 525, 996
TscAI CASTG 3 cut(s) 316, 435, 477
TseFI GTSAC 2 cut(s) 260, 373
TseI GCWGC 4 cut(s) 170, 418, 596, 655
Tsp45I GTSAC 2 cut(s) 260, 373
TspDTI ATGAA 4 cut(s) 24, 29, 413, 681
TspRI CASTG 3 cut(s) 316, 435, 477
Van91I CCANNNNNTGG 1 cut(s) 1013
XmnI GAANNNNTTC 1 cut(s) 562
ZrmI AGTACT 2 cut(s) 404, 868
Zsp2I ATGCAT 2 cut(s) 324, 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.