Rh7BG094000

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001035
Physical Location & Seq
Reverse (-)
6907446 .. 6913071
5626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG094000.1

Sequence Viewer

Length: 942 bp
ATGTCGGCTTTCATGAATCACAATCCGAACAATTCCTTTGAGGTCGCTCAGCCTCCAAGCGATTCTGTTTCAAGCCTCAGTTTCAGTCCCAAATCGAACTTTCTGGTCTCTACCTCCTGGGACAATCAGGTGCGATGCTGGGATATTCAAAGAATTGGAACAAATATTGCTGCTGCGCCGAAGAGCTCCTTTTCGCATGACCATCCGGTTTTGTGCTCAACATGGAAGGATGATGGGATGGCTGTATTCTCTGGAGGTTGTGACAGGCAAGTTAAGATGTGGTCTCTAATGTCTGGTGGCCAAACGAGTACTGTTGCCATGCATGATGCACCCATAAAAGAGATTGCTTGGATTCCCGAGATGGCGCTCTTAGTCACAGGAAGCTGGGACAAGACTATGAAGTACTGGGATTTGAGGCAGCCAAATCCAGTGCATACCCAACAACTACCCGAACGCTGTTATGCACTAACAGTGAAACACCCTCTAATGGTTGTTGGAACAGCAGATAGAAATCTGATCATATTTAACTTGCAGAGTCCACAGACTGAGTTCAAGAGAATCATTTCACCCCTGAAGTATCAGACAAGGTGTGTTGCTGCATTTCCTGATAAGCAAGGTTTCTTGGTTGGTTCAATTGAAGGGAGGGTTGGAGTGCAGCATCTGGATGAAGGACAAGATAGTAAAAACTTTACCTTCAAATGCCACAGAGAAAACAATGCTCTTTACTCAGTCAACTCTATAAACTTCCATCCTGTGCATCACACATTTTCTACAGCTGGTTCTGATGGTTCTATCAGTTTTTGGGATAAGGACAGCAAACAGAGACTCAAGGCCATGCAAAGGTGCAATCAACCCATTCCTTGCAGTACTTTCAACAATGATGGTTCCATATATGCATACTCGGTGATCTCTCTCTCTCTCTGCGCGCGTGTGCTTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

313

Amino Acids

35.04

Weight (kDa)

8.34

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 12 - 154 1.3e-15 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 13 - 146 2.7e-13 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 18 - 168 4.6e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 20 - 48 1.3e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 21 - 145 7e-20 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 22 - 139 3e-09 TEP-1 second beta-propeller
WD40_Gbeta PF25391 33 - 140 2.1e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 64 - 140 1.7e-09 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 65 - 176 3e-15 WDR3 first beta-propeller domain
WDR55 PF24796 65 - 223 1.3e-11 WDR55
Beta-prop_THOC3 PF25174 66 - 301 9.2e-30 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 67 - 149 2.4e-10 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 116 - 223 2.6e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 147 - 283 1.1e-09 WDHD1 first WD40 domain
WD40_Prp19 PF24814 159 - 300 5.2e-12 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 165 - 297 8.6e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 926, 928
AcoI YGGCCR 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 593
AfaI GTAC 3 cut(s) 310, 404, 868
AfiI CCNNNNNNNGG 2 cut(s) 487, 840
AgsI TTSAA 7 cut(s) 72, 149, 553, 633, 638, 697, 874
AjnI CCWGG 1 cut(s) 116
AjuI GAANNNNNNNTTGG 2 cut(s) 630, 662
AluBI AGCT 3 cut(s) 186, 384, 776
AluI AGCT 3 cut(s) 186, 384, 776
Alw21I GWGCWC 2 cut(s) 188, 218
Alw26I GTCTC 3 cut(s) 112, 288, 817
AlwNI CAGNNNCTG 1 cut(s) 661
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 2 cut(s) 298, 831
ApeKI GCWGC 5 cut(s) 170, 173, 418, 596, 655
Asp700I GAANNNNTTC 1 cut(s) 562
AspLEI GCGC 4 cut(s) 178, 367, 926, 928
AsuHPI GGTGA 2 cut(s) 558, 916
AvaI CYCGRG 1 cut(s) 356
BalI TGGCCA 1 cut(s) 300
BanII GRGCYC 1 cut(s) 188
Bbv12I GWGCWC 2 cut(s) 188, 218
BbvI GCAGC 5 cut(s) 157, 160, 430, 583, 667
BccI CCATC 7 cut(s) 210, 227, 232, 355, 756, 779, 875
BciT130I CCWGG 1 cut(s) 118
BclI TGATCA 1 cut(s) 516
BcoDI GTCTC 3 cut(s) 112, 288, 817
BfmI CTRYAG 1 cut(s) 771
BfoI RGCGCY 1 cut(s) 368
BisI GCNGC 5 cut(s) 171, 174, 419, 597, 656
BlpI GCTNAGC 1 cut(s) 48
BlsI GCNGC 5 cut(s) 172, 175, 420, 598, 657
BmcAI AGTACT 3 cut(s) 310, 404, 868
Bme1390I CCNGG 1 cut(s) 118
BmeT110I CYCGRG 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 886
BmrFI CCNGG 1 cut(s) 118
BmrI ACTGGG 1 cut(s) 415
BmsI GCATC 4 cut(s) 125, 316, 667, 766
BmuI ACTGGG 1 cut(s) 415
BpmI CTGGAG 1 cut(s) 273
Bpu1102I GCTNAGC 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 812
BsaBI GATNNNNATC 1 cut(s) 510
BsaI GGTCTC 2 cut(s) 112, 288
BsaJI CCNNGG 1 cut(s) 117
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 2 cut(s) 487, 840
Bse1I ACTGG 2 cut(s) 410, 428
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 5 cut(s) 202, 235, 243, 670, 748
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 2 cut(s) 487, 840
BseMII CTCAG 4 cut(s) 62, 91, 537, 741
BseNI ACTGG 2 cut(s) 410, 428
BsePI GCGCGC 1 cut(s) 924
BseXI GCAGC 5 cut(s) 157, 160, 430, 583, 667
BseYI CCCAGC 2 cut(s) 138, 384
BsgI GTGCAG 1 cut(s) 674
Bsh1236I CGCG 2 cut(s) 926, 928
BshFI GGCC 2 cut(s) 300, 833
BsiHKAI GWGCWC 2 cut(s) 188, 218
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 3 cut(s) 72, 134, 401
BslI CCNNNNNNNGG 2 cut(s) 487, 840
BsmAI GTCTC 3 cut(s) 112, 288, 817
BsmFI GGGAC 3 cut(s) 72, 134, 401
BsnI GGCC 2 cut(s) 300, 833
Bso31I GGTCTC 2 cut(s) 112, 288
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 2 cut(s) 188, 218
Bsp143I GATC 2 cut(s) 516, 906
Bsp1720I GCTNAGC 1 cut(s) 48
BspANI GGCC 2 cut(s) 300, 833
BspCNI CTCAG 4 cut(s) 61, 90, 538, 740
BspFNI CGCG 2 cut(s) 926, 928
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 1 cut(s) 886
BspQI GCTCTTC 1 cut(s) 176
BspTNI GGTCTC 2 cut(s) 112, 288
BsrI ACTGG 2 cut(s) 410, 428
BssECI CCNNGG 1 cut(s) 117
BssHII GCGCGC 1 cut(s) 924
BssMI GATC 2 cut(s) 516, 906
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 2 cut(s) 313, 472
Bst6I CTCTTC 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 926
BstDEI CTNAG 5 cut(s) 48, 77, 370, 546, 727
BstF5I GGATG 5 cut(s) 202, 235, 243, 670, 748
BstFNI CGCG 2 cut(s) 926, 928
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 4 cut(s) 178, 367, 926, 928
BstKTI GATC 2 cut(s) 519, 909
BstMAI GTCTC 3 cut(s) 112, 288, 817
BstMBI GATC 2 cut(s) 516, 906
BstNI CCWGG 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 116
BstSFI CTRYAG 1 cut(s) 771
BstUI CGCG 2 cut(s) 926, 928
BstV1I GCAGC 5 cut(s) 157, 160, 430, 583, 667
BsuRI GGCC 2 cut(s) 300, 833
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 5 cut(s) 202, 235, 243, 670, 748
BtsIMutI CAGTG 2 cut(s) 435, 477
Cac8I GCNNGC 1 cut(s) 926
CaiI CAGNNNCTG 1 cut(s) 661
CciI TCATGA 1 cut(s) 12
CfoI GCGC 4 cut(s) 178, 367, 926, 928
Csp6I GTAC 3 cut(s) 309, 403, 867
CviAII CATG 6 cut(s) 13, 197, 222, 319, 323, 835
CviQI GTAC 3 cut(s) 309, 403, 867
DdeI CTNAG 5 cut(s) 48, 77, 370, 546, 727
DpnI GATC 2 cut(s) 518, 908
DpnII GATC 2 cut(s) 516, 906
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 2 cut(s) 112, 288
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 116
EcoT22I ATGCAT 2 cut(s) 324, 898
EcoT38I GRGCYC 1 cut(s) 188
FaeI CATG 6 cut(s) 16, 200, 225, 322, 326, 838
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 3 cut(s) 72, 134, 401
FatI CATG 6 cut(s) 12, 196, 221, 318, 322, 834
FbaI TGATCA 1 cut(s) 516
Fnu4HI GCNGC 5 cut(s) 171, 174, 419, 597, 656
FokI GGATG 5 cut(s) 189, 242, 250, 677, 735
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 5 cut(s) 171, 174, 419, 597, 656
GlaI GCGC 4 cut(s) 177, 366, 925, 927
GluI GCNGC 5 cut(s) 171, 174, 419, 597, 656
GsaI CCCAGC 2 cut(s) 142, 388
GsuI CTGGAG 1 cut(s) 273
HaeII RGCGCY 1 cut(s) 368
HaeIII GGCC 2 cut(s) 300, 833
HapII CCGG 1 cut(s) 206
HhaI GCGC 4 cut(s) 178, 367, 926, 928
Hin1II CATG 6 cut(s) 16, 200, 225, 322, 326, 838
Hin6I GCGC 4 cut(s) 176, 365, 924, 926
HinP1I GCGC 4 cut(s) 176, 365, 924, 926
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HinfI GANTC 6 cut(s) 16, 62, 352, 535, 558, 825
HpaII CCGG 1 cut(s) 206
HphI GGTGA 2 cut(s) 558, 916
Hpy166II GTNNAC 2 cut(s) 539, 733
Hpy188I TCNGA 4 cut(s) 27, 516, 582, 784
Hpy188III TCNNGA 6 cut(s) 13, 252, 356, 553, 605, 662
Hpy8I GTNNAC 2 cut(s) 539, 733
HpyAV CCTTC 4 cut(s) 220, 632, 662, 703
HpyCH4III ACNGT 2 cut(s) 313, 472
HpyF3I CTNAG 5 cut(s) 48, 77, 370, 546, 727
Hsp92II CATG 6 cut(s) 16, 200, 225, 322, 326, 838
HspAI GCGC 4 cut(s) 176, 365, 924, 926
Ksp22I TGATCA 1 cut(s) 516
Kzo9I GATC 2 cut(s) 516, 906
LguI GCTCTTC 1 cut(s) 176
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 5 cut(s) 157, 160, 430, 583, 667
LweI GCATC 4 cut(s) 125, 316, 667, 766
MaeIII GTNAC 2 cut(s) 260, 373
MalI GATC 2 cut(s) 518, 908
MboI GATC 2 cut(s) 516, 906
MboII GAAGA 1 cut(s) 193
MfeI CAATTG 1 cut(s) 633
MhlI GDGCHC 2 cut(s) 188, 218
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 3 cut(s) 31, 153, 633
MluNI TGGCCA 1 cut(s) 300
MlyI GAGTC 2 cut(s) 544, 819
MmeI TCCRAC 2 cut(s) 475, 628
MnlI CCTC 8 cut(s) 34, 63, 86, 124, 248, 408, 492, 636
Mox20I TGGCCA 1 cut(s) 300
Mph1103I ATGCAT 2 cut(s) 324, 898
MroXI GAANNNNTTC 1 cut(s) 562
MscI TGGCCA 1 cut(s) 300
MseI TTAA 2 cut(s) 273, 525
MslI CAYNNNNRTG 1 cut(s) 663
Msp20I TGGCCA 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 1 cut(s) 118
MunI CAATTG 1 cut(s) 633
MvaI CCWGG 1 cut(s) 118
MvnI CGCG 2 cut(s) 926, 928
NdeII GATC 2 cut(s) 516, 906
NlaIII CATG 6 cut(s) 16, 200, 225, 322, 326, 838
NlaIV GGNNCC 1 cut(s) 886
NmuCI GTSAC 2 cut(s) 260, 373
NsiI ATGCAT 2 cut(s) 324, 898
PagI TCATGA 1 cut(s) 12
PauI GCGCGC 1 cut(s) 924
PciSI GCTCTTC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 562
PfeI GAWTC 4 cut(s) 16, 62, 352, 558
PkrI GCNGC 5 cut(s) 172, 175, 420, 598, 657
PleI GAGTC 2 cut(s) 543, 819
PpsI GAGTC 2 cut(s) 543, 819
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 116
PspFI CCCAGC 2 cut(s) 138, 384
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 886
PstNI CAGNNNCTG 1 cut(s) 661
PteI GCGCGC 1 cut(s) 924
PvuII CAGCTG 1 cut(s) 776
RsaI GTAC 3 cut(s) 310, 404, 868
RsaNI GTAC 3 cut(s) 309, 403, 867
RseI CAYNNNNRTG 1 cut(s) 663
SacI GAGCTC 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 176
SaqAI TTAA 2 cut(s) 273, 525
SatI GCNGC 5 cut(s) 171, 174, 419, 597, 656
Sau3AI GATC 2 cut(s) 516, 906
ScaI AGTACT 3 cut(s) 310, 404, 868
SchI GAGTC 2 cut(s) 544, 819
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 2 cut(s) 188, 218
SfaNI GCATC 4 cut(s) 125, 316, 667, 766
SfcI CTRYAG 1 cut(s) 771
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
Sse9I AATT 3 cut(s) 31, 153, 633
SspI AATATT 1 cut(s) 166
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 1 cut(s) 116
TaaI ACNGT 2 cut(s) 313, 472
TaqI TCGA 1 cut(s) 95
TasI AATT 3 cut(s) 31, 153, 633
TatI WGTACW 3 cut(s) 308, 402, 866
TfiI GAWTC 4 cut(s) 16, 62, 352, 558
Tru1I TTAA 2 cut(s) 273, 525
Tru9I TTAA 2 cut(s) 273, 525
TscAI CASTG 2 cut(s) 435, 477
TseFI GTSAC 2 cut(s) 260, 373
TseI GCWGC 5 cut(s) 170, 173, 418, 596, 655
Tsp45I GTSAC 2 cut(s) 260, 373
TspDTI ATGAA 3 cut(s) 29, 413, 681
TspRI CASTG 2 cut(s) 435, 477
XmnI GAANNNNTTC 1 cut(s) 562
ZrmI AGTACT 3 cut(s) 310, 404, 868
Zsp2I ATGCAT 2 cut(s) 324, 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.