Rroxscaffold_3G00233330

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001035
Physical Location & Seq
Forward (+)
19273509 .. 19281631
8123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00233330.1

Sequence Viewer

Length: 1197 bp
ATGTCGACGTTCATGGCCGCTTCCAATCCAAATCCAAACAAATCCTTTGAGGTCGCTCAGCCCCCGACCGACTCCGTTTCGAGTCTCAGCTTCAGCCCTAAGGCTAACTTTCTGGTCGCAACCTCCTGGGACAACCAGGTGCGATGTTGGGAGATACAACAGAGTGGTGGAAATGTTGCCAGTATGCCCAAGGCAGCTATATCGCACGATAATCCGGTTTTGTGCTCAACTTGGAAAGATGATGGGACAACTGTGTTTTCTGGAGGTTGTGACAAGCAAGTTAAGATGTGGCCTCTGATGTCTGGTGGCAACCAAACAATGACTGTTGCAATGCATGATGCGCCCATCAAGGAGATAGCTTGGATCCCTGAGATGAGCCTCTTGGTCACAGGAAGCTGGGACAAGACTTTGAAGTATTGGGATACAAGGCAGCCAAATCCAGTGCATACCCAACTACTCCCAGAGAGGTGCTATGCATTATCAGTGAGACACCCTCTGATGGTGGTTGGAACTGCTGATCGAAATCTTATTGTTTATAACTTGCAGAGTCCACAGACTGAGTTCAAGAGAATCATTTCACCCCTAAAGTATCAGACAAGGTGTGTTGCGGCATTTCCTGATCAGCAAGGATTCTTGGTTCTTAGCATTGCTGTTTTCTGGATAGCATTGCTGTGCTCTGGATCCTGGCAGCACTCAGAGGTTGGCTCTATTGAAGGGAGGGTTGGTGTACATCATCTGGATGAGGCACAACAAAGTAAAAACTTTACCTTCAAATGCCACAGAGAGGGCAACGAGATATACTCAGTCAATTCTCTGAACTTCCATCCTGTGTATCATACATTTGCTACTGCTGGTTCTGATGGTTCTTTCAACTTTTGGGACAAGGACAGCAAACAGAGACTAAAGGCCATGGCAAGGTGCAGTCAACCAATACCTTGCAGTACTTTCAACAACGATGGTTCCATATTTGCATACTCGTTAATAATAGAGGAAGCATTGCACATTGGCTTTAATGAAACGTTTGCTATGATTGGAGTAAGGGCGCAGAAAATCACAATCCGGCCACAGCAAAGAACTACATTTACCTCCATGTTCCACAGGAATCTGAGGTCAAAAGCAAGCCACGAATCTCAACTGGTGGAAGAAAGTGAAGCTGGTCCTGATTCTCTTGATCAGACATGCTATTGTATAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

398

Amino Acids

44.5

Weight (kDa)

6.66

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 13 - 149 2.4e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 20 - 181 1.1e-18 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 20 - 171 1.7e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 23 - 50 7.3e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 23 - 150 2.4e-19 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 23 - 142 1.7e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 24 - 179 1.3e-11 WDR3 second beta-propeller domain
WDR55 PF24796 24 - 207 4.7e-14 WDR55
WD40_Gbeta PF25391 26 - 145 4.3e-09 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 68 - 185 9.1e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 69 - 326 1.6e-24 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 69 - 154 6.3e-10 WDHD1 first WD40 domain
WD40 PF00400 105 - 141 1e-05 WD domain, G-beta repeat
WD40_Prp19 PF24814 164 - 325 8e-09 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 537
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 18, 608
AclI AACGTT 1 cut(s) 1019
AclWI GGATC 4 cut(s) 358, 371, 675, 688
AcoI YGGCCR 2 cut(s) 15, 1061
AcuI CTGAAG 1 cut(s) 76
AfaI GTAC 2 cut(s) 729, 943
AfiI CCNNNNNNNGG 3 cut(s) 499, 784, 915
AgsI TTSAA 6 cut(s) 412, 565, 713, 772, 871, 949
AjnI CCWGG 3 cut(s) 125, 135, 683
AjuI GAANNNNNNNTTGG 2 cut(s) 705, 737
AloI GAACNNNNNNTCC 2 cut(s) 621, 653
AluBI AGCT 6 cut(s) 90, 197, 359, 396, 1154, 1194
AluI AGCT 6 cut(s) 90, 197, 359, 396, 1154, 1194
Alw21I GWGCWC 2 cut(s) 227, 677
Alw26I GTCTC 3 cut(s) 89, 481, 892
AlwI GGATC 4 cut(s) 358, 371, 675, 688
AoxI GGCC 4 cut(s) 15, 290, 906, 1061
ApeKI GCWGC 3 cut(s) 194, 430, 688
Asp700I GAANNNNTTC 1 cut(s) 574
AspLEI GCGC 2 cut(s) 343, 1045
AspS9I GGNCC 1 cut(s) 1157
AsuHPI GGTGA 1 cut(s) 570
AvaII GGWCC 1 cut(s) 1157
AxyI CCTNAGG 1 cut(s) 99
BamHI GGATCC 2 cut(s) 363, 680
Bbv12I GWGCWC 2 cut(s) 227, 677
BbvI GCAGC 3 cut(s) 206, 442, 700
BccI CCATC 6 cut(s) 236, 353, 493, 831, 854, 950
BcgI CGANNNNNNTGC 2 cut(s) 183, 217
BciT130I CCWGG 3 cut(s) 127, 137, 685
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 2 cut(s) 619, 1171
BcoDI GTCTC 3 cut(s) 89, 481, 892
BfaI CTAG 1 cut(s) 1195
BfuI GTATCC 1 cut(s) 415
BisI GCNGC 5 cut(s) 18, 195, 431, 609, 689
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 5 cut(s) 19, 196, 432, 610, 690
BmcAI AGTACT 1 cut(s) 943
Bme1390I CCNGG 3 cut(s) 127, 137, 685
Bme18I GGWCC 1 cut(s) 1157
BmgT120I GGNCC 1 cut(s) 1157
BmiI GGNNCC 3 cut(s) 365, 682, 961
BmrFI CCNGG 3 cut(s) 127, 137, 685
BmsI GCATC 1 cut(s) 328
BplI GAGNNNNNCTC 6 cut(s) 478, 510, 689, 721, 785, 817
BpmI CTGGAG 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 57
BsaBI GATNNNNATC 1 cut(s) 522
BsaJI CCNNGG 3 cut(s) 126, 189, 909
BsaWI WCCGGW 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 709, 739
Bsc4I CCNNNNNNNGG 3 cut(s) 499, 784, 915
Bse1I ACTGG 3 cut(s) 180, 440, 1140
Bse21I CCTNAGG 1 cut(s) 99
Bse3DI GCAATG 4 cut(s) 336, 645, 665, 995
Bse8I GATNNNNATC 1 cut(s) 522
BseBI CCWGG 3 cut(s) 127, 137, 685
BseDI CCNNGG 3 cut(s) 126, 189, 909
BseGI GGATG 2 cut(s) 745, 823
BseJI GATNNNNATC 1 cut(s) 522
BseLI CCNNNNNNNGG 3 cut(s) 499, 784, 915
BseMI GCAATG 4 cut(s) 336, 645, 665, 995
BseMII CTCAG 7 cut(s) 71, 100, 360, 549, 708, 816, 1097
BseNI ACTGG 3 cut(s) 180, 440, 1140
BseXI GCAGC 3 cut(s) 206, 442, 700
BseYI CCCAGC 1 cut(s) 396
BsgI GTGCAG 1 cut(s) 940
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 4 cut(s) 17, 292, 908, 1063
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 2 cut(s) 227, 677
BsiSI CCGG 2 cut(s) 215, 1060
BslFI GGGAC 4 cut(s) 143, 259, 413, 893
BslI CCNNNNNNNGG 3 cut(s) 499, 784, 915
BsmAI GTCTC 3 cut(s) 89, 481, 892
BsmFI GGGAC 4 cut(s) 143, 259, 413, 893
BsnI GGCC 4 cut(s) 17, 292, 908, 1063
Bsp1286I GDGCHC 2 cut(s) 227, 677
Bsp1407I TGTACA 1 cut(s) 727
Bsp143I GATC 5 cut(s) 363, 517, 619, 680, 1171
Bsp1720I GCTNAGC 1 cut(s) 57
Bsp19I CCATGG 1 cut(s) 909
BspACI CCGC 2 cut(s) 18, 608
BspANI GGCC 4 cut(s) 17, 292, 908, 1063
BspCNI CTCAG 7 cut(s) 70, 99, 361, 550, 707, 815, 1098
BspLI GGNNCC 3 cut(s) 365, 682, 961
BspPI GGATC 4 cut(s) 358, 371, 675, 688
BsrDI GCAATG 4 cut(s) 336, 645, 665, 995
BsrGI TGTACA 1 cut(s) 727
BsrI ACTGG 3 cut(s) 180, 440, 1140
BssECI CCNNGG 3 cut(s) 126, 189, 909
BssMI GATC 5 cut(s) 363, 517, 619, 680, 1171
BssT1I CCWWGG 2 cut(s) 189, 909
Bst2UI CCWGG 3 cut(s) 127, 137, 685
Bst4CI ACNGT 2 cut(s) 253, 325
BstAUI TGTACA 1 cut(s) 727
BstC8I GCNNGC 1 cut(s) 1120
BstDEI CTNAG 9 cut(s) 57, 86, 99, 369, 558, 641, 694, 802, 1106
BstDSI CCRYGG 1 cut(s) 909
BstF5I GGATG 2 cut(s) 745, 823
BstHHI GCGC 2 cut(s) 343, 1045
BstKTI GATC 5 cut(s) 366, 520, 622, 683, 1174
BstMAI GTCTC 3 cut(s) 89, 481, 892
BstMBI GATC 5 cut(s) 363, 517, 619, 680, 1171
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstNI CCWGG 3 cut(s) 127, 137, 685
BstNSI RCATGY 1 cut(s) 1182
BstSCI CCNGG 3 cut(s) 125, 135, 683
BstV1I GCAGC 3 cut(s) 206, 442, 700
BstX2I RGATCY 2 cut(s) 363, 680
BstYI RGATCY 2 cut(s) 363, 680
Bsu36I CCTNAGG 1 cut(s) 99
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 4 cut(s) 17, 292, 908, 1063
BtgI CCRYGG 1 cut(s) 909
BtgZI GCGATG 1 cut(s) 157
BtsCI GGATG 2 cut(s) 745, 823
BtsIMutI CAGTG 2 cut(s) 447, 489
Cac8I GCNNGC 1 cut(s) 1120
CfoI GCGC 2 cut(s) 343, 1045
Cfr13I GGNCC 1 cut(s) 1157
CsiI ACCWGGT 1 cut(s) 135
Csp6I GTAC 2 cut(s) 728, 942
CviAII CATG 5 cut(s) 13, 335, 910, 1090, 1179
CviQI GTAC 2 cut(s) 728, 942
DdeI CTNAG 9 cut(s) 57, 86, 99, 369, 558, 641, 694, 802, 1106
DpnI GATC 5 cut(s) 365, 519, 621, 682, 1173
DpnII GATC 5 cut(s) 363, 517, 619, 680, 1171
EaeI YGGCCR 2 cut(s) 15, 1061
Eco130I CCWWGG 2 cut(s) 189, 909
Eco47I GGWCC 1 cut(s) 1157
Eco57I CTGAAG 1 cut(s) 76
Eco81I CCTNAGG 1 cut(s) 99
EcoRII CCWGG 3 cut(s) 125, 135, 683
EcoT14I CCWWGG 2 cut(s) 189, 909
EcoT22I ATGCAT 2 cut(s) 336, 478
ErhI CCWWGG 2 cut(s) 189, 909
FaeI CATG 5 cut(s) 16, 338, 913, 1093, 1182
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FaqI GGGAC 4 cut(s) 143, 259, 413, 893
FatI CATG 5 cut(s) 12, 334, 909, 1089, 1178
FbaI TGATCA 2 cut(s) 619, 1171
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 5 cut(s) 18, 195, 431, 609, 689
FokI GGATG 2 cut(s) 752, 810
Fsp4HI GCNGC 5 cut(s) 18, 195, 431, 609, 689
FspBI CTAG 1 cut(s) 1195
GlaI GCGC 2 cut(s) 342, 1044
GluI GCNGC 5 cut(s) 18, 195, 431, 609, 689
GsaI CCCAGC 1 cut(s) 400
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 4 cut(s) 17, 292, 908, 1063
HapII CCGG 2 cut(s) 215, 1060
HhaI GCGC 2 cut(s) 343, 1045
Hin1II CATG 5 cut(s) 16, 338, 913, 1093, 1182
Hin6I GCGC 2 cut(s) 341, 1043
HinP1I GCGC 2 cut(s) 341, 1043
HincII GTYRAC 2 cut(s) 6, 926
HindII GTYRAC 2 cut(s) 6, 926
HinfI GANTC 8 cut(s) 71, 82, 547, 570, 630, 1102, 1127, 1163
HpaII CCGG 2 cut(s) 215, 1060
HphI GGTGA 1 cut(s) 570
Hpy166II GTNNAC 4 cut(s) 6, 551, 728, 926
Hpy188I TCNGA 8 cut(s) 297, 498, 594, 697, 816, 859, 1107, 1176
Hpy188III TCNNGA 8 cut(s) 261, 565, 617, 658, 678, 737, 1160, 1169
Hpy8I GTNNAC 4 cut(s) 6, 551, 728, 926
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 707, 778
HpyCH4III ACNGT 2 cut(s) 253, 325
HpyCH4IV ACGT 2 cut(s) 8, 1019
HpyCH4V TGCA 9 cut(s) 329, 334, 445, 476, 544, 921, 939, 971, 1000
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpyF3I CTNAG 9 cut(s) 57, 86, 99, 369, 558, 641, 694, 802, 1106
HpySE526I ACGT 2 cut(s) 8, 1019
Hsp92II CATG 5 cut(s) 16, 338, 913, 1093, 1182
HspAI GCGC 2 cut(s) 341, 1043
Ksp22I TGATCA 2 cut(s) 619, 1171
Kzo9I GATC 5 cut(s) 363, 517, 619, 680, 1171
Lsp1109I GCAGC 3 cut(s) 206, 442, 700
LweI GCATC 1 cut(s) 328
MabI ACCWGGT 1 cut(s) 135
MaeI CTAG 1 cut(s) 1195
MaeII ACGT 2 cut(s) 8, 1019
MaeIII GTNAC 2 cut(s) 269, 385
MalI GATC 5 cut(s) 365, 519, 621, 682, 1173
MboI GATC 5 cut(s) 363, 517, 619, 680, 1171
MboII GAAGA 1 cut(s) 1154
MflI RGATCY 2 cut(s) 363, 680
MhlI GDGCHC 2 cut(s) 227, 677
MluCI AATT 1 cut(s) 808
MlyI GAGTC 3 cut(s) 65, 91, 556
MmeI TCCRAC 1 cut(s) 487
Mph1103I ATGCAT 2 cut(s) 336, 478
MroXI GAANNNNTTC 1 cut(s) 574
MseI TTAA 3 cut(s) 282, 980, 1011
MslI CAYNNNNRTG 2 cut(s) 670, 738
MspI CCGG 2 cut(s) 215, 1060
MspR9I CCNGG 3 cut(s) 127, 137, 685
MvaI CCWGG 3 cut(s) 127, 137, 685
MwoI GCNNNNNNNGC 1 cut(s) 340
NcoI CCATGG 1 cut(s) 909
NdeII GATC 5 cut(s) 363, 517, 619, 680, 1171
NlaIII CATG 5 cut(s) 16, 338, 913, 1093, 1182
NlaIV GGNNCC 3 cut(s) 365, 682, 961
NmuCI GTSAC 2 cut(s) 269, 385
NsiI ATGCAT 2 cut(s) 336, 478
NspI RCATGY 1 cut(s) 1182
PdmI GAANNNNTTC 1 cut(s) 574
PfeI GAWTC 5 cut(s) 570, 630, 1102, 1127, 1163
PkrI GCNGC 5 cut(s) 19, 196, 432, 610, 690
PleI GAGTC 3 cut(s) 65, 90, 555
PpsI GAGTC 3 cut(s) 65, 90, 555
PsiI TTATAA 1 cut(s) 537
Psp1406I AACGTT 1 cut(s) 1019
Psp6I CCWGG 3 cut(s) 125, 135, 683
PspFI CCCAGC 1 cut(s) 396
PspGI CCWGG 3 cut(s) 125, 135, 683
PspN4I GGNNCC 3 cut(s) 365, 682, 961
PspPI GGNCC 1 cut(s) 1157
PsrI GAACNNNNNNTAC 2 cut(s) 1066, 1098
PsuI RGATCY 2 cut(s) 363, 680
RsaI GTAC 2 cut(s) 729, 943
RsaNI GTAC 2 cut(s) 728, 942
RseI CAYNNNNRTG 2 cut(s) 670, 738
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 282, 980, 1011
SatI GCNGC 5 cut(s) 18, 195, 431, 609, 689
Sau3AI GATC 5 cut(s) 363, 517, 619, 680, 1171
Sau96I GGNCC 1 cut(s) 1157
ScaI AGTACT 1 cut(s) 943
SchI GAGTC 3 cut(s) 65, 91, 556
ScrFI CCNGG 3 cut(s) 127, 137, 685
SduI GDGCHC 2 cut(s) 227, 677
SexAI ACCWGGT 1 cut(s) 135
SfaNI GCATC 1 cut(s) 328
SinI GGWCC 1 cut(s) 1157
SmiMI CAYNNNNRTG 2 cut(s) 670, 738
Sse9I AATT 1 cut(s) 808
SsiI CCGC 2 cut(s) 18, 608
SspMI CTAG 1 cut(s) 1195
StyD4I CCNGG 3 cut(s) 125, 135, 683
StyI CCWWGG 2 cut(s) 189, 909
TaaI ACNGT 2 cut(s) 253, 325
TaiI ACGT 2 cut(s) 11, 1022
TaqI TCGA 3 cut(s) 5, 80, 520
TaqII GACCGA 1 cut(s) 83
TasI AATT 1 cut(s) 808
TatI WGTACW 2 cut(s) 727, 941
TauI GCSGC 2 cut(s) 20, 611
TfiI GAWTC 5 cut(s) 570, 630, 1102, 1127, 1163
Tru1I TTAA 3 cut(s) 282, 980, 1011
Tru9I TTAA 3 cut(s) 282, 980, 1011
TscAI CASTG 2 cut(s) 447, 489
TseFI GTSAC 2 cut(s) 269, 385
TseI GCWGC 3 cut(s) 194, 430, 688
Tsp45I GTSAC 2 cut(s) 269, 385
TspDTI ATGAA 1 cut(s) 1029
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 2 cut(s) 447, 489
VpaK11BI GGWCC 1 cut(s) 1157
XceI RCATGY 1 cut(s) 1182
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 574
XspI CTAG 1 cut(s) 1195
ZrmI AGTACT 1 cut(s) 943
Zsp2I ATGCAT 2 cut(s) 336, 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.