pycom08g19220

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001035
Physical Location & Seq
Forward (+)
19241738 .. 19246829
5092 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g19220.3

Sequence Viewer

Length: 1176 bp
ATGACCTTTGTGGAAGAGCTCCCTCCTTTTTCCTCTTGGACCAAAGTTTCAAGGCTCAGTCTTCTTCTCACATATTCCGCTCAGTTTCCTCTCCTCTCTCCTCCTTCAGCTAACGTTCTGAGTGCGAGTTCAATGTCGAATTTCATGGCTGCTTCGAATCAGAATACGAATCCGAACAAGTCCTTTGAGGTCACCCCTCCACCGAACGACTCGATATCGAGCCTAAGCTTCAGTCCCAAGAGTAACATTCTGGTCGCCACCTCCTGGGATAACCAGGTACGATGCTGGGAGATACACCAGAGTGGACAAAATCTTGCTAGTGTGCCCAAGGCATCCATATCGCATGACCAACCGGTTTTGTGCTCAACCTGGAAGGATGATGGATCGACAGTGTTCTCCGGAGGGTGCGACAAGCAAGTTAAGATGTGGCCTTTGGCTGGTAACCAACCAGTGACCGTTGCCATGCATGAAGCACCCATAAAAGAGATTGCATGGATCCCAGAGATGAACTACTTAGTCACAGGAAGCTGGGACAAGACTTTGAAGTATTGGGATCTGAGGCAGCAAACTCCAATCCATACCCAACCTCTACCTGAGCGCTGCTACACTCTATCAGTGAGATACCCTCTAATGGTTGTTGGCACTGCAGACAGAAATCTGATTGTTTTTAACTTGCAAAACCCACAGACAGAATTCAAGAGAATTGTTTCACCCCTAAAGTATCAAACAAGGTGTGTTGCTGCATTTCCTGATCAGCAAGGATTCTTGGTTGGTTCTATTGAAGGGAGGGTTGGTGTACATCATCTGGATGATGGACTACAAGGTAAAAACTTTACCTTCAAGTGCCATAGAGAGGGCAATGAGATATACTCAGTCAACTCTCTGAACTTCCATCCAGTCCATCACACATTTGCTACCGCTGGTTCTGACGGTTCTTTCAATTTTTGGGATAAGGATAGCAAACAGAGACTAAAGGCAATGGCGAGGTGCAGTCAACCTATACCTTGCAGTGCTTTCAACAGTGATGGTTCCATATTTGCATACGCAGTTTGCTATGATTGGAGCAAGGGCGCTGAAAATCATAATCCTGCTACCGCAAAGAACTACATTTACCTCCACGTGCCCCAGGAAGCCGAGGTTAAAGGCAAGCCAAGAATCAACGCTGGAAAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

392

Amino Acids

43.63

Weight (kDa)

8.48

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 264
AccBSI CCGCTC 1 cut(s) 80
AccIII TCCGGA 1 cut(s) 398
AciI CCGC 3 cut(s) 78, 918, 1095
AclI AACGTT 1 cut(s) 114
AclWI GGATC 4 cut(s) 391, 490, 503, 561
AcsI RAATTY 2 cut(s) 139, 692
AcuI CTGAAG 2 cut(s) 90, 214
AcvI CACGTG 1 cut(s) 1120
AfaI GTAC 2 cut(s) 279, 798
AfeI AGCGCT 1 cut(s) 599
AfiI CCNNNNNNNGG 4 cut(s) 264, 437, 631, 853
AgeI ACCGGT 1 cut(s) 352
AgsI TTSAA 8 cut(s) 51, 132, 544, 697, 782, 841, 940, 1018
AjnI CCWGG 4 cut(s) 263, 273, 368, 1125
AjuI GAANNNNNNNTTGG 2 cut(s) 774, 806
AleI CACNNNNGTG 1 cut(s) 300
AluBI AGCT 4 cut(s) 19, 110, 228, 528
AluI AGCT 4 cut(s) 19, 110, 228, 528
Alw21I GWGCWC 2 cut(s) 21, 365
Alw26I GTCTC 1 cut(s) 961
AlwI GGATC 4 cut(s) 391, 490, 503, 561
Aor13HI TCCGGA 1 cut(s) 398
Aor51HI AGCGCT 1 cut(s) 599
AoxI GGCC 1 cut(s) 428
ApeKI GCWGC 4 cut(s) 149, 562, 600, 740
ApoI RAATTY 2 cut(s) 139, 692
AsiGI ACCGGT 1 cut(s) 352
Asp700I GAANNNNTTC 1 cut(s) 706
AspLEI GCGC 2 cut(s) 600, 1073
AspS9I GGNCC 1 cut(s) 39
AsuHPI GGTGA 2 cut(s) 184, 702
AsuII TTCGAA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 39
BaeGI GKGCMC 2 cut(s) 327, 1125
BamHI GGATCC 1 cut(s) 495
BanII GRGCYC 1 cut(s) 21
BbrPI CACGTG 1 cut(s) 1120
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 2 cut(s) 21, 365
BbvI GCAGC 4 cut(s) 136, 574, 587, 727
BccI CCATC 5 cut(s) 374, 806, 900, 909, 1019
BcgI CGANNNNNNTGC 2 cut(s) 321, 355
BciT130I CCWGG 4 cut(s) 265, 275, 370, 1127
BclI TGATCA 1 cut(s) 751
BcoDI GTCTC 1 cut(s) 961
BfaI CTAG 1 cut(s) 318
BfmI CTRYAG 1 cut(s) 645
BfoI RGCGCY 2 cut(s) 601, 1074
BisI GCNGC 4 cut(s) 150, 563, 601, 741
BlsI GCNGC 4 cut(s) 151, 564, 602, 742
Bme1390I CCNGG 4 cut(s) 265, 275, 370, 1127
Bme18I GGWCC 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 39
BmiI GGNNCC 2 cut(s) 497, 1030
BmrFI CCNGG 4 cut(s) 265, 275, 370, 1127
BmsI GCATC 2 cut(s) 272, 341
BpiI GAAGAC 1 cut(s) 53
BplI GAGNNNNNCTC 4 cut(s) 610, 642, 854, 886
Bpu10I CCTNAGC 2 cut(s) 224, 594
Bpu14I TTCGAA 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 1120
BsaJI CCNNGG 4 cut(s) 264, 327, 1125, 1134
BsaWI WCCGGW 2 cut(s) 352, 398
BsaXI ACNNNNNCTCC 2 cut(s) 778, 808
Bsc4I CCNNNNNNNGG 4 cut(s) 264, 437, 631, 853
Bse118I RCCGGY 1 cut(s) 352
Bse1I ACTGG 2 cut(s) 449, 896
Bse3DI GCAATG 2 cut(s) 865, 984
BseAI TCCGGA 1 cut(s) 398
BseBI CCWGG 4 cut(s) 265, 275, 370, 1127
BseDI CCNNGG 4 cut(s) 264, 327, 1125, 1134
BseGI GGATG 4 cut(s) 332, 382, 814, 892
BseLI CCNNNNNNNGG 4 cut(s) 264, 437, 631, 853
BseMI GCAATG 2 cut(s) 865, 984
BseMII CTCAG 6 cut(s) 70, 95, 110, 548, 585, 885
BseNI ACTGG 2 cut(s) 449, 896
BseRI GAGGAG 2 cut(s) 83, 90
BseSI GKGCMC 2 cut(s) 327, 1125
BseXI GCAGC 4 cut(s) 136, 574, 587, 727
BseYI CCCAGC 2 cut(s) 285, 528
BsgI GTGCAG 1 cut(s) 1009
BshFI GGCC 1 cut(s) 430
BshTI ACCGGT 1 cut(s) 352
BsiHKAI GWGCWC 2 cut(s) 21, 365
BsiSI CCGG 2 cut(s) 353, 399
BslFI GGGAC 2 cut(s) 219, 545
BslI CCNNNNNNNGG 4 cut(s) 264, 437, 631, 853
BsmAI GTCTC 1 cut(s) 961
BsmFI GGGAC 2 cut(s) 219, 545
BsnI GGCC 1 cut(s) 430
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 4 cut(s) 21, 327, 365, 1125
Bsp13I TCCGGA 1 cut(s) 398
Bsp1407I TGTACA 1 cut(s) 796
Bsp143I GATC 4 cut(s) 383, 495, 553, 751
BspACI CCGC 3 cut(s) 78, 918, 1095
BspANI GGCC 1 cut(s) 430
BspCNI CTCAG 6 cut(s) 69, 94, 111, 549, 586, 884
BspEI TCCGGA 1 cut(s) 398
BspLI GGNNCC 2 cut(s) 497, 1030
BspMAI CTGCAG 1 cut(s) 649
BspPI GGATC 4 cut(s) 391, 490, 503, 561
BspQI GCTCTTC 1 cut(s) 9
BspT104I TTCGAA 1 cut(s) 155
BsrBI CCGCTC 1 cut(s) 80
BsrDI GCAATG 2 cut(s) 865, 984
BsrFI RCCGGY 1 cut(s) 352
BsrGI TGTACA 1 cut(s) 796
BsrI ACTGG 2 cut(s) 449, 896
BssAI RCCGGY 1 cut(s) 352
BssECI CCNNGG 4 cut(s) 264, 327, 1125, 1134
BssMI GATC 4 cut(s) 383, 495, 553, 751
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 4 cut(s) 265, 275, 370, 1127
Bst4CI ACNGT 5 cut(s) 391, 457, 932, 1022, 1173
Bst6I CTCTTC 1 cut(s) 9
BstAUI TGTACA 1 cut(s) 796
BstBAI YACGTR 1 cut(s) 1120
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 1148
BstDEI CTNAG 8 cut(s) 56, 81, 119, 224, 514, 557, 594, 871
BstEII GGTNACC 2 cut(s) 190, 440
BstF5I GGATG 4 cut(s) 332, 382, 814, 892
BstH2I RGCGCY 2 cut(s) 601, 1074
BstHHI GCGC 2 cut(s) 600, 1073
BstKTI GATC 4 cut(s) 386, 498, 556, 754
BstMAI GTCTC 1 cut(s) 961
BstMBI GATC 4 cut(s) 383, 495, 553, 751
BstNI CCWGG 4 cut(s) 265, 275, 370, 1127
BstPI GGTNACC 2 cut(s) 190, 440
BstSCI CCNGG 4 cut(s) 263, 273, 368, 1125
BstSFI CTRYAG 1 cut(s) 645
BstSLI GKGCMC 2 cut(s) 327, 1125
BstV1I GCAGC 4 cut(s) 136, 574, 587, 727
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 2 cut(s) 495, 553
BstYI RGATCY 2 cut(s) 495, 553
BsuRI GGCC 1 cut(s) 430
BtsCI GGATG 4 cut(s) 332, 382, 814, 892
BtsI GCAGTG 2 cut(s) 642, 1015
BtsIMutI CAGTG 6 cut(s) 396, 456, 621, 642, 1015, 1027
Cac8I GCNNGC 1 cut(s) 1148
CfoI GCGC 2 cut(s) 600, 1073
Cfr10I RCCGGY 1 cut(s) 352
Cfr13I GGNCC 1 cut(s) 39
CsiI ACCWGGT 1 cut(s) 273
Csp6I GTAC 2 cut(s) 278, 797
CspAI ACCGGT 1 cut(s) 352
CviAII CATG 5 cut(s) 145, 344, 463, 467, 492
CviQI GTAC 2 cut(s) 278, 797
DdeI CTNAG 8 cut(s) 56, 81, 119, 224, 514, 557, 594, 871
DpnI GATC 4 cut(s) 385, 497, 555, 753
DpnII GATC 4 cut(s) 383, 495, 553, 751
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
Ecl136II GAGCTC 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 21
Eco32I GATATC 1 cut(s) 216
Eco47I GGWCC 1 cut(s) 39
Eco47III AGCGCT 1 cut(s) 599
Eco53kI GAGCTC 1 cut(s) 19
Eco57I CTGAAG 2 cut(s) 90, 214
Eco72I CACGTG 1 cut(s) 1120
Eco91I GGTNACC 2 cut(s) 190, 440
EcoICRI GAGCTC 1 cut(s) 19
EcoO65I GGTNACC 2 cut(s) 190, 440
EcoRI GAATTC 1 cut(s) 692
EcoRII CCWGG 4 cut(s) 263, 273, 368, 1125
EcoRV GATATC 1 cut(s) 216
EcoT14I CCWWGG 1 cut(s) 327
EcoT22I ATGCAT 1 cut(s) 468
EcoT38I GRGCYC 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 5 cut(s) 148, 347, 466, 470, 495
FaqI GGGAC 2 cut(s) 219, 545
FatI CATG 5 cut(s) 144, 343, 462, 466, 491
FbaI TGATCA 1 cut(s) 751
Fnu4HI GCNGC 4 cut(s) 150, 563, 601, 741
FokI GGATG 4 cut(s) 319, 389, 821, 879
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 4 cut(s) 150, 563, 601, 741
FspBI CTAG 1 cut(s) 318
GlaI GCGC 2 cut(s) 599, 1072
GluI GCNGC 4 cut(s) 150, 563, 601, 741
GsaI CCCAGC 2 cut(s) 289, 532
HaeII RGCGCY 2 cut(s) 601, 1074
HaeIII GGCC 1 cut(s) 430
HapII CCGG 2 cut(s) 353, 399
HhaI GCGC 2 cut(s) 600, 1073
Hin1II CATG 5 cut(s) 148, 347, 466, 470, 495
Hin6I GCGC 2 cut(s) 598, 1071
HinP1I GCGC 2 cut(s) 598, 1071
HincII GTYRAC 2 cut(s) 877, 995
HindII GTYRAC 2 cut(s) 877, 995
HindIII AAGCTT 1 cut(s) 226
HinfI GANTC 5 cut(s) 157, 169, 209, 762, 1155
HpaII CCGG 2 cut(s) 353, 399
HphI GGTGA 2 cut(s) 184, 702
Hpy166II GTNNAC 4 cut(s) 305, 797, 877, 995
Hpy188I TCNGA 7 cut(s) 120, 162, 174, 558, 660, 885, 928
Hpy188III TCNNGA 4 cut(s) 399, 697, 749, 806
Hpy8I GTNNAC 4 cut(s) 305, 797, 877, 995
HpyAV CCTTC 4 cut(s) 114, 367, 776, 847
HpyCH4III ACNGT 5 cut(s) 391, 457, 932, 1022, 1173
HpyCH4IV ACGT 2 cut(s) 114, 1119
HpyCH4V TGCA 8 cut(s) 466, 491, 647, 676, 743, 990, 1008, 1040
HpyF3I CTNAG 8 cut(s) 56, 81, 119, 224, 514, 557, 594, 871
HpySE526I ACGT 2 cut(s) 114, 1119
Hsp92II CATG 5 cut(s) 148, 347, 466, 470, 495
HspAI GCGC 2 cut(s) 598, 1071
Kpn2I TCCGGA 1 cut(s) 398
Ksp22I TGATCA 1 cut(s) 751
Kzo9I GATC 4 cut(s) 383, 495, 553, 751
LguI GCTCTTC 1 cut(s) 9
LmnI GCTCC 2 cut(s) 24, 1062
Lsp1109I GCAGC 4 cut(s) 136, 574, 587, 727
LweI GCATC 2 cut(s) 272, 341
MabI ACCWGGT 1 cut(s) 273
MaeI CTAG 1 cut(s) 318
MaeII ACGT 2 cut(s) 114, 1119
MaeIII GTNAC 5 cut(s) 190, 242, 440, 451, 517
MalI GATC 4 cut(s) 385, 497, 555, 753
MbiI CCGCTC 1 cut(s) 80
MboI GATC 4 cut(s) 383, 495, 553, 751
MboII GAAGA 3 cut(s) 26, 53, 56
MflI RGATCY 2 cut(s) 495, 553
MhlI GDGCHC 4 cut(s) 21, 327, 365, 1125
MluCI AATT 4 cut(s) 139, 692, 702, 940
MlyI GAGTC 1 cut(s) 203
Mph1103I ATGCAT 1 cut(s) 468
MroI TCCGGA 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 706
MseI TTAA 3 cut(s) 420, 669, 1140
MslI CAYNNNNRTG 2 cut(s) 300, 807
MspA1I CMGCKG 1 cut(s) 920
MspI CCGG 2 cut(s) 353, 399
MspR9I CCNGG 4 cut(s) 265, 275, 370, 1127
MvaI CCWGG 4 cut(s) 265, 275, 370, 1127
NdeII GATC 4 cut(s) 383, 495, 553, 751
NlaIII CATG 5 cut(s) 148, 347, 466, 470, 495
NlaIV GGNNCC 2 cut(s) 497, 1030
NmeAIII GCCGAG 1 cut(s) 1159
NmuCI GTSAC 3 cut(s) 190, 451, 517
NsiI ATGCAT 1 cut(s) 468
NspV TTCGAA 1 cut(s) 155
OliI CACNNNNGTG 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 9
PdmI GAANNNNTTC 1 cut(s) 706
PfeI GAWTC 4 cut(s) 157, 169, 762, 1155
PflMI CCANNNNNTGG 1 cut(s) 264
PinAI ACCGGT 1 cut(s) 352
PkrI GCNGC 4 cut(s) 151, 564, 602, 742
PleI GAGTC 1 cut(s) 203
PmaCI CACGTG 1 cut(s) 1120
PmlI CACGTG 1 cut(s) 1120
PpsI GAGTC 1 cut(s) 203
Ppu21I YACGTR 1 cut(s) 1120
Psp124BI GAGCTC 1 cut(s) 21
Psp1406I AACGTT 1 cut(s) 114
Psp6I CCWGG 4 cut(s) 263, 273, 368, 1125
PspCI CACGTG 1 cut(s) 1120
PspEI GGTNACC 2 cut(s) 190, 440
PspFI CCCAGC 2 cut(s) 285, 528
PspGI CCWGG 4 cut(s) 263, 273, 368, 1125
PspN4I GGNNCC 2 cut(s) 497, 1030
PspPI GGNCC 1 cut(s) 39
PsrI GAACNNNNNNTAC 2 cut(s) 1094, 1126
PstI CTGCAG 1 cut(s) 649
PsuI RGATCY 2 cut(s) 495, 553
RsaI GTAC 2 cut(s) 279, 798
RsaNI GTAC 2 cut(s) 278, 797
RseI CAYNNNNRTG 2 cut(s) 300, 807
SacI GAGCTC 1 cut(s) 21
SapI GCTCTTC 1 cut(s) 9
SaqAI TTAA 3 cut(s) 420, 669, 1140
SatI GCNGC 4 cut(s) 150, 563, 601, 741
Sau3AI GATC 4 cut(s) 383, 495, 553, 751
Sau96I GGNCC 1 cut(s) 39
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 4 cut(s) 265, 275, 370, 1127
SduI GDGCHC 4 cut(s) 21, 327, 365, 1125
SexAI ACCWGGT 1 cut(s) 273
SfaNI GCATC 2 cut(s) 272, 341
SfcI CTRYAG 1 cut(s) 645
SfuI TTCGAA 1 cut(s) 155
SinI GGWCC 1 cut(s) 39
SmiMI CAYNNNNRTG 2 cut(s) 300, 807
Sse9I AATT 4 cut(s) 139, 692, 702, 940
SsiI CCGC 3 cut(s) 78, 918, 1095
SspMI CTAG 1 cut(s) 318
SstI GAGCTC 1 cut(s) 21
StyD4I CCNGG 4 cut(s) 263, 273, 368, 1125
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 5 cut(s) 391, 457, 932, 1022, 1173
TaiI ACGT 2 cut(s) 117, 1122
TaqI TCGA 5 cut(s) 137, 155, 212, 218, 386
TasI AATT 4 cut(s) 139, 692, 702, 940
TatI WGTACW 1 cut(s) 796
TfiI GAWTC 4 cut(s) 157, 169, 762, 1155
Tru1I TTAA 3 cut(s) 420, 669, 1140
Tru9I TTAA 3 cut(s) 420, 669, 1140
TscAI CASTG 6 cut(s) 396, 456, 621, 649, 1015, 1027
TseFI GTSAC 3 cut(s) 190, 451, 517
TseI GCWGC 4 cut(s) 149, 562, 600, 740
Tsp45I GTSAC 3 cut(s) 190, 451, 517
TspDTI ATGAA 3 cut(s) 133, 483, 521
TspRI CASTG 6 cut(s) 396, 456, 621, 649, 1015, 1027
Van91I CCANNNNNTGG 1 cut(s) 264
VpaK11BI GGWCC 1 cut(s) 39
XapI RAATTY 2 cut(s) 139, 692
XmnI GAANNNNTTC 1 cut(s) 706
XspI CTAG 1 cut(s) 318
Zsp2I ATGCAT 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.