Rh2BG266700

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001035
Physical Location & Seq
Reverse (-)
28653815 .. 28658121
4307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG266700.1

Sequence Viewer

Length: 1128 bp
ATGAGAGACATTAAAGTAATAAAAACCCAGAAAAACAGTAAGCTCCCGCTCCAGCTTTCGAAAACAAAAAGCAAGGTCGTAAAATCCACGCTAATACGACCACCTAAAGTAGCAGCTTCTCCGATGTCGAGTTTCATGCCGGCCGCCAATCAGAACACGAATCCCAACAAGTCCTTCGAGGTCGCCGAACCTCCAAACGACTCCATTTCGAGCCTCAGCTTCAGCCCCAAAGCCAATCACCTCGTCGCCACCTCCTGGGACAACCAGGTTCGATGCTGGGAGATACAACACAGTGGACAAAATCTTGCTAGCACCCCCAAGGGAATGATTTCACACGACCATCCGGCTTTGTGCTCAACTTGGAAGGATGATGGGGCGACTGTCTTCTCTGGAGGATGTGACAAGCAAGCTAAGATGTGGTCCTTGGCTACTAACCAAACAATGACTGTTGCCGTGCATGATGCCCCCATTAAAGACATGGCTTGGATCCCCGAGTTGAACCTCCTAGTCACTGGAAGCTTGGACAAGACTGTAAAGTATTGGGATACGAGGCAGCAGAGCCCAGCGCACACCCAACAGCTCCCTGAACGCTGCTATGCACTATCAGTGAAACAGTCTCTAATGGTTGTTGGGACGGCGGATAGAAATATTATTATTTTTCACTTGCAGCAACCCCAGAATGAGTTGAAGAGAATTGTTTCACCCCTAAAGTTTCAGACAAGGTGTGTTGCTGCATTCCCTGATCAGCAAGGATTCTTGGTTGGATCTATTGAGGGGAGGGTTGGTGTGCATCATCTGGATGAGCAACAAGTATCTAAAAACTTTACCTTCAAATGCCACAGGGAGGGCAATGAGATATACTCTGTCAACTCTCTGAACTTCCATCCTGTCCACCACACGTTTGCTACCGCTGGCTCTGATGGTTCTTTTAATTTTTGGGACAAGGACAGCAAACAAAGACTCAAGGCAATGCAAAGGTGCAGTCAGCCTATACCTTGCAGTACCTTTAACCACGATGGTTCCATATATGCATATGCGGTTTGCTACGATTGGAGCAAGGGTGCAGAAAATCATAATCCTGCGGCAGCAAAGAACCACATTTTCCTCCATTTGCCACAGGTATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

375

Amino Acids

41.91

Weight (kDa)

9.11

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 61 - 221 2.8e-19 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 62 - 190 2e-11 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 63 - 217 2.1e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 66 - 277 6.6e-22 WDR5 beta-propeller domain
WDR55 PF24796 66 - 269 6.4e-17 WDR55
Beta-prop_WDR3_2nd PF25172 67 - 221 1.8e-09 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 68 - 227 1.2e-06 Aladin seven-bladed propeller
WD40_Gbeta PF25391 78 - 185 1.3e-07 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 113 - 194 5.4e-09 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 119 - 346 9.7e-31 THOC3 beta-propeller domain
WD40_Prp19 PF24814 122 - 192 2.3e-09 Prp19 WD40 domain
WD40 PF00400 147 - 182 5.1e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 161 - 280 3.7e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 193 - 327 8.6e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 200 - 345 1.4e-13 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 202 - 325 2.4e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 209 - 341 5.7e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 255
AccBSI CCGCTC 1 cut(s) 49
AciI CCGC 6 cut(s) 47, 144, 638, 909, 1037, 1082
AclWI GGATC 3 cut(s) 481, 494, 772
AcoI YGGCCR 1 cut(s) 141
AcuI CTGAAG 1 cut(s) 205
AfaI GTAC 1 cut(s) 1003
AfiI CCNNNNNNNGG 2 cut(s) 255, 844
AflIII ACRYGT 1 cut(s) 897
AgsI TTSAA 3 cut(s) 499, 688, 832
AjnI CCWGG 2 cut(s) 254, 264
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AluBI AGCT 7 cut(s) 43, 55, 116, 219, 410, 519, 580
AluI AGCT 7 cut(s) 43, 55, 116, 219, 410, 519, 580
Alw21I GWGCWC 1 cut(s) 356
Alw26I GTCTC 1 cut(s) 621
AlwI GGATC 3 cut(s) 481, 494, 772
Ama87I CYCGRG 1 cut(s) 491
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 6 cut(s) 113, 553, 591, 667, 731, 1085
ArsI GACNNNNNNTTYG 2 cut(s) 967, 999
Asp700I GAANNNNTTC 2 cut(s) 328, 697
AspLEI GCGC 1 cut(s) 568
AspS9I GGNCC 1 cut(s) 420
AsuHPI GGTGA 2 cut(s) 230, 693
AsuII TTCGAA 1 cut(s) 59
AsuNHI GCTAGC 1 cut(s) 308
AvaI CYCGRG 1 cut(s) 491
AvaII GGWCC 1 cut(s) 420
BamHI GGATCC 1 cut(s) 486
BanII GRGCYC 1 cut(s) 563
BbsI GAAGAC 1 cut(s) 376
Bbv12I GWGCWC 1 cut(s) 356
BbvCI CCTCAGC 1 cut(s) 215
BbvI GCAGC 6 cut(s) 125, 565, 578, 679, 718, 1097
BccI CCATC 5 cut(s) 348, 365, 891, 914, 1010
BceAI ACGGC 2 cut(s) 437, 651
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 2 cut(s) 256, 266
BciVI GTATCC 1 cut(s) 538
BclI TGATCA 1 cut(s) 742
BcoDI GTCTC 1 cut(s) 621
BfaI CTAG 2 cut(s) 309, 506
BfuI GTATCC 1 cut(s) 538
BisI GCNGC 8 cut(s) 114, 144, 554, 592, 668, 732, 1083, 1086
BlsI GCNGC 8 cut(s) 115, 145, 555, 593, 669, 733, 1084, 1087
Bme1390I CCNGG 2 cut(s) 256, 266
Bme18I GGWCC 1 cut(s) 420
BmeT110I CYCGRG 1 cut(s) 491
BmgT120I GGNCC 1 cut(s) 420
BmiI GGNNCC 2 cut(s) 488, 1021
BmrFI CCNGG 2 cut(s) 256, 266
BmsI GCATC 3 cut(s) 263, 451, 799
BmtI GCTAGC 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 376
BplI GAGNNNNNCTC 2 cut(s) 845, 877
BpmI CTGGAG 2 cut(s) 35, 411
Bpu10I CCTNAGC 1 cut(s) 215
Bpu14I TTCGAA 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 947
BsaJI CCNNGG 3 cut(s) 255, 318, 423
BsaXI ACNNNNNCTCC 4 cut(s) 769, 799, 1045, 1075
Bsc4I CCNNNNNNNGG 2 cut(s) 255, 844
Bse118I RCCGGY 1 cut(s) 139
Bse1I ACTGG 1 cut(s) 517
Bse3DI GCAATG 2 cut(s) 856, 975
BseBI CCWGG 2 cut(s) 256, 266
BseDI CCNNGG 3 cut(s) 255, 318, 423
BseGI GGATG 5 cut(s) 340, 373, 401, 805, 883
BseLI CCNNNNNNNGG 2 cut(s) 255, 844
BseMI GCAATG 2 cut(s) 856, 975
BseMII CTCAG 1 cut(s) 229
BseNI ACTGG 1 cut(s) 517
BseX3I CGGCCG 1 cut(s) 141
BseXI GCAGC 6 cut(s) 125, 565, 578, 679, 718, 1097
BseYI CCCAGC 2 cut(s) 276, 562
BsgI GTGCAG 2 cut(s) 1000, 1083
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 1 cut(s) 143
BsiEI CGRYCG 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 356
BsiHKCI CYCGRG 1 cut(s) 491
BsiSI CCGG 2 cut(s) 140, 344
BslFI GGGAC 3 cut(s) 272, 646, 953
BslI CCNNNNNNNGG 2 cut(s) 255, 844
BsmAI GTCTC 1 cut(s) 621
BsmFI GGGAC 3 cut(s) 272, 646, 953
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 1 cut(s) 143
BsoBI CYCGRG 1 cut(s) 491
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 2 cut(s) 356, 563
Bsp143I GATC 3 cut(s) 486, 742, 764
BspACI CCGC 6 cut(s) 47, 144, 638, 909, 1037, 1082
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 1 cut(s) 228
BspLI GGNNCC 2 cut(s) 488, 1021
BspOI GCTAGC 1 cut(s) 312
BspPI GGATC 3 cut(s) 481, 494, 772
BspT104I TTCGAA 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 49
BsrDI GCAATG 2 cut(s) 856, 975
BsrFI RCCGGY 1 cut(s) 139
BsrI ACTGG 1 cut(s) 517
BssAI RCCGGY 1 cut(s) 139
BssECI CCNNGG 3 cut(s) 255, 318, 423
BssMI GATC 3 cut(s) 486, 742, 764
BssT1I CCWWGG 2 cut(s) 318, 423
Bst2UI CCWGG 2 cut(s) 256, 266
Bst4CI ACNGT 6 cut(s) 38, 293, 382, 448, 532, 615
Bst6I CTCTTC 1 cut(s) 683
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 4 cut(s) 141, 310, 408, 913
BstDEI CTNAG 2 cut(s) 215, 411
BstF5I GGATG 5 cut(s) 340, 373, 401, 805, 883
BstHHI GCGC 1 cut(s) 568
BstKTI GATC 3 cut(s) 489, 745, 767
BstMAI GTCTC 1 cut(s) 621
BstMBI GATC 3 cut(s) 486, 742, 764
BstMCI CGRYCG 1 cut(s) 144
BstNI CCWGG 2 cut(s) 256, 266
BstSCI CCNGG 2 cut(s) 254, 264
BstV1I GCAGC 6 cut(s) 125, 565, 578, 679, 718, 1097
BstV2I GAAGAC 1 cut(s) 376
BstX2I RGATCY 2 cut(s) 486, 764
BstYI RGATCY 2 cut(s) 486, 764
BstZI CGGCCG 1 cut(s) 141
BsuI GTATCC 1 cut(s) 538
BsuRI GGCC 1 cut(s) 143
BtsCI GGATG 5 cut(s) 340, 373, 401, 805, 883
BtsIMutI CAGTG 3 cut(s) 298, 510, 612
Cac8I GCNNGC 4 cut(s) 141, 310, 408, 913
CfoI GCGC 1 cut(s) 568
Cfr10I RCCGGY 1 cut(s) 139
Cfr13I GGNCC 1 cut(s) 420
CsiI ACCWGGT 1 cut(s) 264
Csp6I GTAC 1 cut(s) 1002
CviAII CATG 3 cut(s) 136, 458, 478
CviQI GTAC 1 cut(s) 1002
DdeI CTNAG 2 cut(s) 215, 411
DpnI GATC 3 cut(s) 488, 744, 766
DpnII GATC 3 cut(s) 486, 742, 764
EaeI YGGCCR 1 cut(s) 141
EagI CGGCCG 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 683
EarI CTCTTC 1 cut(s) 683
EciI GGCGGA 1 cut(s) 653
EclXI CGGCCG 1 cut(s) 141
Eco130I CCWWGG 2 cut(s) 318, 423
Eco24I GRGCYC 1 cut(s) 563
Eco47I GGWCC 1 cut(s) 420
Eco52I CGGCCG 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 491
EcoRII CCWGG 2 cut(s) 254, 264
EcoT14I CCWWGG 2 cut(s) 318, 423
EcoT22I ATGCAT 1 cut(s) 1033
EcoT38I GRGCYC 1 cut(s) 563
ErhI CCWWGG 2 cut(s) 318, 423
FaeI CATG 3 cut(s) 139, 461, 481
FaqI GGGAC 3 cut(s) 272, 646, 953
FatI CATG 3 cut(s) 135, 457, 477
FauI CCCGC 1 cut(s) 54
FauNDI CATATG 1 cut(s) 1033
FbaI TGATCA 1 cut(s) 742
Fnu4HI GCNGC 8 cut(s) 114, 144, 554, 592, 668, 732, 1083, 1086
FokI GGATG 5 cut(s) 327, 380, 408, 812, 870
FriOI GRGCYC 1 cut(s) 563
Fsp4HI GCNGC 8 cut(s) 114, 144, 554, 592, 668, 732, 1083, 1086
FspBI CTAG 2 cut(s) 309, 506
GlaI GCGC 1 cut(s) 567
GluI GCNGC 8 cut(s) 114, 144, 554, 592, 668, 732, 1083, 1086
GsaI CCCAGC 2 cut(s) 280, 566
GsuI CTGGAG 2 cut(s) 35, 411
HaeIII GGCC 1 cut(s) 143
HapII CCGG 2 cut(s) 140, 344
HhaI GCGC 1 cut(s) 568
Hin1II CATG 3 cut(s) 139, 461, 481
Hin6I GCGC 1 cut(s) 566
HinP1I GCGC 1 cut(s) 566
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HindIII AAGCTT 1 cut(s) 517
HinfI GANTC 4 cut(s) 160, 200, 753, 960
HpaII CCGG 2 cut(s) 140, 344
HphI GGTGA 2 cut(s) 230, 693
Hpy166II GTNNAC 3 cut(s) 296, 868, 892
Hpy188I TCNGA 5 cut(s) 123, 153, 717, 876, 919
Hpy188III TCNNGA 2 cut(s) 390, 797
Hpy8I GTNNAC 3 cut(s) 296, 868, 892
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 3 cut(s) 184, 358, 838
HpyCH4III ACNGT 6 cut(s) 38, 293, 382, 448, 532, 615
HpyCH4IV ACGT 1 cut(s) 899
HpyF3I CTNAG 2 cut(s) 215, 411
HpySE526I ACGT 1 cut(s) 899
Hsp92II CATG 3 cut(s) 139, 461, 481
HspAI GCGC 1 cut(s) 566
KroI GCCGGC 1 cut(s) 139
KroNI GCCGGC 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 3 cut(s) 486, 742, 764
LmnI GCTCC 4 cut(s) 48, 54, 585, 1053
Lsp1109I GCAGC 6 cut(s) 125, 565, 578, 679, 718, 1097
LweI GCATC 3 cut(s) 263, 451, 799
MabI ACCWGGT 1 cut(s) 264
MaeI CTAG 2 cut(s) 309, 506
MaeII ACGT 1 cut(s) 899
MaeIII GTNAC 2 cut(s) 398, 508
MalI GATC 3 cut(s) 488, 744, 766
MbiI CCGCTC 1 cut(s) 49
MboI GATC 3 cut(s) 486, 742, 764
MboII GAAGA 2 cut(s) 376, 700
MflI RGATCY 2 cut(s) 486, 764
MhlI GDGCHC 2 cut(s) 356, 563
MluCI AATT 2 cut(s) 693, 931
MlyI GAGTC 2 cut(s) 194, 954
MmeI TCCRAC 1 cut(s) 742
Mph1103I ATGCAT 1 cut(s) 1033
MroNI GCCGGC 1 cut(s) 139
MroXI GAANNNNTTC 2 cut(s) 328, 697
MseI TTAA 4 cut(s) 12, 471, 930, 1008
MslI CAYNNNNRTG 1 cut(s) 798
MspA1I CMGCKG 1 cut(s) 911
MspI CCGG 2 cut(s) 140, 344
MspR9I CCNGG 2 cut(s) 256, 266
Mva1269I GAATGC 1 cut(s) 734
MvaI CCWGG 2 cut(s) 256, 266
NaeI GCCGGC 1 cut(s) 141
NdeI CATATG 1 cut(s) 1033
NdeII GATC 3 cut(s) 486, 742, 764
NgoMIV GCCGGC 1 cut(s) 139
NheI GCTAGC 1 cut(s) 308
NlaIII CATG 3 cut(s) 139, 461, 481
NlaIV GGNNCC 2 cut(s) 488, 1021
NmuCI GTSAC 2 cut(s) 398, 508
NsiI ATGCAT 1 cut(s) 1033
NspV TTCGAA 1 cut(s) 59
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 734
PdiI GCCGGC 1 cut(s) 141
PdmI GAANNNNTTC 2 cut(s) 328, 697
PfeI GAWTC 2 cut(s) 160, 753
PflMI CCANNNNNTGG 1 cut(s) 255
PkrI GCNGC 8 cut(s) 115, 145, 555, 593, 669, 733, 1084, 1087
PleI GAGTC 2 cut(s) 194, 954
PpsI GAGTC 2 cut(s) 194, 954
Psp6I CCWGG 2 cut(s) 254, 264
PspFI CCCAGC 2 cut(s) 276, 562
PspGI CCWGG 2 cut(s) 254, 264
PspN4I GGNNCC 2 cut(s) 488, 1021
PspPI GGNCC 1 cut(s) 420
PsuI RGATCY 2 cut(s) 486, 764
RsaI GTAC 1 cut(s) 1003
RsaNI GTAC 1 cut(s) 1002
RseI CAYNNNNRTG 1 cut(s) 798
SaqAI TTAA 4 cut(s) 12, 471, 930, 1008
SatI GCNGC 8 cut(s) 114, 144, 554, 592, 668, 732, 1083, 1086
Sau3AI GATC 3 cut(s) 486, 742, 764
Sau96I GGNCC 1 cut(s) 420
SchI GAGTC 2 cut(s) 194, 954
ScrFI CCNGG 2 cut(s) 256, 266
SduI GDGCHC 2 cut(s) 356, 563
SexAI ACCWGGT 1 cut(s) 264
SfaNI GCATC 3 cut(s) 263, 451, 799
SfuI TTCGAA 1 cut(s) 59
SinI GGWCC 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 798
SmlI CTYRAG 1 cut(s) 962
SmoI CTYRAG 1 cut(s) 962
Sse9I AATT 2 cut(s) 693, 931
SsiI CCGC 6 cut(s) 47, 144, 638, 909, 1037, 1082
SspI AATATT 1 cut(s) 649
SspMI CTAG 2 cut(s) 309, 506
StyD4I CCNGG 2 cut(s) 254, 264
StyI CCWWGG 2 cut(s) 318, 423
TaaI ACNGT 6 cut(s) 38, 293, 382, 448, 532, 615
TaiI ACGT 1 cut(s) 902
TaqI TCGA 5 cut(s) 59, 128, 177, 209, 271
TasI AATT 2 cut(s) 693, 931
TauI GCSGC 2 cut(s) 146, 1085
TfiI GAWTC 2 cut(s) 160, 753
Tru1I TTAA 4 cut(s) 12, 471, 930, 1008
Tru9I TTAA 4 cut(s) 12, 471, 930, 1008
TscAI CASTG 3 cut(s) 298, 517, 612
TseFI GTSAC 2 cut(s) 398, 508
TseI GCWGC 6 cut(s) 113, 553, 591, 667, 731, 1085
Tsp45I GTSAC 2 cut(s) 398, 508
TspDTI ATGAA 1 cut(s) 124
TspRI CASTG 3 cut(s) 298, 517, 612
Van91I CCANNNNNTGG 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 420
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XmnI GAANNNNTTC 2 cut(s) 328, 697
XspI CTAG 2 cut(s) 309, 506
Zsp2I ATGCAT 1 cut(s) 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.