FvH4_5g30850

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001035
Physical Location & Seq
Reverse (-)
21710554 .. 21718402
7849 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30850.t1

Sequence Viewer

Length: 1047 bp
ATGTCGACGTTCATGTCCGCTTCCAATCCAAATCCCAACAAATCCTTCGAGGTCGCTCAGCCCCCAACCGACTCCGTTTCCAGTCTCAGTTTCAGCCCCAAGGCCAACCTACTCGTCGCAACCTCTTGGGACAATCAGGTGCGGTGTTGGGAGATACAGCAGAGTGGAGGAAATGTTGCCAGTATGCCCAAGGCAGCCATTTCGCATGATCAGCCGGTTTTGTGCTCAACTTGGAAAGATGATGGGACAACCGTGTTCTCTGGGGGTTGTGATAAGCAAGTTAAGATGTGGCCTCTGATGTCTGGTGGCAACCAAGCAATGACTGTTGCAATGCATGATGCGCCCATTAAGGAGATTGCTTGGATCCCTGAGATGAGCCTCTTAGTGACAGGAAGCTGGGACAAGACCCTGAAGTATTGGGATACAAGGCAGCCAAATCCAGTGCATACCCAACAGCTCCCGGAGCGCTGCTATGCTTTATCAGTGCGACACCCTCTGATGGTGGTTGGAACTGCCGATCGAAATCTTATTGTTTATAACTTGCAGAATCCACAGGCTGAGTTCAAGAGAATCGTTTCACCCCTAAAGTATCAGACAAGGTGTGTTGCTGCATTTCCTGATCAGCAAGGATTCTTGGTTGGCTCTATTGAAGGGAGGGTTGGTGTACATCATCTGGATGAGGCACAGCAAAGTAAAAACTTTACCTTCAAGTGTCACAGAGAGGGCAACGAGATCTACTCAGTCAATTCACTGAACTTCCATCCTGTGTACCACACCTTTGCTACTGCTGGTTCTGATGGTTCTTTCAACTTTTGGGACAAGGACAGCAAACAGAGATTAAAGGCCATGTCAAGGTGCAGTCAACCAATACCTTGCAGTACCTTCAACAATGACGGTTCCATATTTGCATACTCGGTTTGCTATGATTGGAGCAAGGGCGCAGAAAATCATAACCCGGCTACAGCGAAGAACTACATTTACCTCCATGTTCCACAGGAATCTGAGGTCAAAAGCAAGCCACGAATCTCAACTGGTGGAAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

349

Amino Acids

38.83

Weight (kDa)

8.49

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 14 - 151 2.7e-14 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 19 - 171 1.7e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 20 - 181 3.9e-19 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 23 - 142 1.8e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 24 - 233 9.9e-21 WDR5 beta-propeller domain
WDR55 PF24796 24 - 232 2e-18 WDR55
Beta-prop_WDR3_2nd PF25172 24 - 179 2e-12 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 27 - 144 6.8e-08 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 68 - 152 4.3e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 69 - 305 4.5e-31 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 69 - 155 3.4e-10 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 120 - 234 3.5e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 152 - 286 3.1e-09 WDHD1 first WD40 domain
WD40_Prp19 PF24814 163 - 304 8.8e-13 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 166 - 308 2.4e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 169 - 301 7.2e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 537
AasI GACNNNNNNGTC 1 cut(s) 13
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 18, 142
AclWI GGATC 2 cut(s) 358, 371
AcuI CTGAAG 1 cut(s) 431
AfaI GTAC 3 cut(s) 666, 770, 880
AfeI AGCGCT 1 cut(s) 467
AfiI CCNNNNNNNGG 2 cut(s) 499, 852
AgsI TTSAA 5 cut(s) 565, 650, 709, 808, 886
AjuI GAANNNNNNNTTGG 4 cut(s) 29, 61, 642, 674
AluBI AGCT 2 cut(s) 396, 457
AluI AGCT 2 cut(s) 396, 457
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 2 cut(s) 358, 371
Aor51HI AGCGCT 1 cut(s) 467
AoxI GGCC 3 cut(s) 102, 290, 843
ApeKI GCWGC 4 cut(s) 194, 430, 468, 608
Asp700I GAANNNNTTC 1 cut(s) 574
AspLEI GCGC 3 cut(s) 343, 468, 941
AsuC2I CCSGG 2 cut(s) 461, 956
AsuHPI GGTGA 1 cut(s) 570
BamHI GGATCC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 4 cut(s) 206, 442, 455, 595
BccI CCATC 4 cut(s) 236, 493, 768, 791
BcgI CGANNNNNNTGC 2 cut(s) 183, 217
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 2 cut(s) 208, 619
BcnI CCSGG 2 cut(s) 461, 956
BcoDI GTCTC 1 cut(s) 89
BfmI CTRYAG 1 cut(s) 960
BfoI RGCGCY 1 cut(s) 469
BfuI GTATCC 1 cut(s) 415
BglII AGATCT 1 cut(s) 732
BisI GCNGC 4 cut(s) 195, 431, 469, 609
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 4 cut(s) 196, 432, 470, 610
Bme1390I CCNGG 2 cut(s) 461, 956
BmiI GGNNCC 2 cut(s) 365, 898
BmrFI CCNGG 2 cut(s) 461, 956
BmsI GCATC 1 cut(s) 328
BplI GAGNNNNNCTC 2 cut(s) 722, 754
Bpu1102I GCTNAGC 1 cut(s) 57
BpuMI CCSGG 2 cut(s) 461, 956
BsaBI GATNNNNATC 1 cut(s) 522
BsaJI CCNNGG 2 cut(s) 99, 189
BsaXI ACNNNNNCTCC 4 cut(s) 159, 189, 646, 676
Bsc4I CCNNNNNNNGG 2 cut(s) 499, 852
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 4 cut(s) 81, 180, 440, 1036
Bse3DI GCAATG 2 cut(s) 324, 336
Bse8I GATNNNNATC 1 cut(s) 522
BseDI CCNNGG 2 cut(s) 99, 189
BseGI GGATG 2 cut(s) 682, 760
BseJI GATNNNNATC 1 cut(s) 522
BseLI CCNNNNNNNGG 2 cut(s) 499, 852
BseMI GCAATG 2 cut(s) 324, 336
BseMII CTCAG 6 cut(s) 71, 100, 360, 549, 753, 993
BseNI ACTGG 4 cut(s) 81, 180, 440, 1036
BseXI GCAGC 4 cut(s) 206, 442, 455, 595
BseYI CCCAGC 1 cut(s) 396
BsgI GTGCAG 1 cut(s) 877
Bsh1285I CGRYCG 1 cut(s) 520
BshFI GGCC 3 cut(s) 104, 292, 845
BsiEI CGRYCG 1 cut(s) 520
BsiHKAI GWGCWC 1 cut(s) 227
BsiSI CCGG 3 cut(s) 215, 461, 956
BslFI GGGAC 4 cut(s) 143, 259, 413, 830
BslI CCNNNNNNNGG 2 cut(s) 499, 852
BsmAI GTCTC 1 cut(s) 89
BsmFI GGGAC 4 cut(s) 143, 259, 413, 830
BsnI GGCC 3 cut(s) 104, 292, 845
Bsp1286I GDGCHC 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 664
Bsp143I GATC 5 cut(s) 208, 363, 517, 619, 732
Bsp1720I GCTNAGC 1 cut(s) 57
BspACI CCGC 2 cut(s) 18, 142
BspANI GGCC 3 cut(s) 104, 292, 845
BspCNI CTCAG 6 cut(s) 70, 99, 361, 550, 752, 994
BspLI GGNNCC 2 cut(s) 365, 898
BspPI GGATC 2 cut(s) 358, 371
BsrDI GCAATG 2 cut(s) 324, 336
BsrFI RCCGGY 1 cut(s) 214
BsrGI TGTACA 1 cut(s) 664
BsrI ACTGG 4 cut(s) 81, 180, 440, 1036
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 2 cut(s) 99, 189
BssMI GATC 5 cut(s) 208, 363, 517, 619, 732
BssT1I CCWWGG 2 cut(s) 99, 189
Bst4CI ACNGT 3 cut(s) 253, 325, 896
BstAUI TGTACA 1 cut(s) 664
BstC8I GCNNGC 1 cut(s) 1016
BstDEI CTNAG 7 cut(s) 57, 86, 369, 382, 558, 739, 1002
BstF5I GGATG 2 cut(s) 682, 760
BstH2I RGCGCY 1 cut(s) 469
BstHHI GCGC 3 cut(s) 343, 468, 941
BstKTI GATC 5 cut(s) 211, 366, 520, 622, 735
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 5 cut(s) 208, 363, 517, 619, 732
BstMCI CGRYCG 1 cut(s) 520
BstMWI GCNNNNNNNGC 3 cut(s) 211, 340, 463
BstSCI CCNGG 2 cut(s) 459, 954
BstSFI CTRYAG 1 cut(s) 960
BstV1I GCAGC 4 cut(s) 206, 442, 455, 595
BstX2I RGATCY 2 cut(s) 363, 732
BstYI RGATCY 2 cut(s) 363, 732
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 3 cut(s) 104, 292, 845
BtsCI GGATG 2 cut(s) 682, 760
BtsIMutI CAGTG 3 cut(s) 447, 489, 749
Cac8I GCNNGC 1 cut(s) 1016
CfoI GCGC 3 cut(s) 343, 468, 941
Cfr10I RCCGGY 1 cut(s) 214
Csp6I GTAC 3 cut(s) 665, 769, 879
CviAII CATG 5 cut(s) 13, 206, 335, 847, 986
CviQI GTAC 3 cut(s) 665, 769, 879
DdeI CTNAG 7 cut(s) 57, 86, 369, 382, 558, 739, 1002
DpnI GATC 5 cut(s) 210, 365, 519, 621, 734
DpnII GATC 5 cut(s) 208, 363, 517, 619, 732
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Eco130I CCWWGG 2 cut(s) 99, 189
Eco47III AGCGCT 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 431
EcoT14I CCWWGG 2 cut(s) 99, 189
EcoT22I ATGCAT 1 cut(s) 336
ErhI CCWWGG 2 cut(s) 99, 189
FaeI CATG 5 cut(s) 16, 209, 338, 850, 989
FaqI GGGAC 4 cut(s) 143, 259, 413, 830
FatI CATG 5 cut(s) 12, 205, 334, 846, 985
FbaI TGATCA 2 cut(s) 208, 619
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 195, 431, 469, 609
FokI GGATG 2 cut(s) 689, 747
Fsp4HI GCNGC 4 cut(s) 195, 431, 469, 609
GlaI GCGC 3 cut(s) 342, 467, 940
GluI GCNGC 4 cut(s) 195, 431, 469, 609
GsaI CCCAGC 1 cut(s) 400
HaeII RGCGCY 1 cut(s) 469
HaeIII GGCC 3 cut(s) 104, 292, 845
HapII CCGG 3 cut(s) 215, 461, 956
HhaI GCGC 3 cut(s) 343, 468, 941
Hin1II CATG 5 cut(s) 16, 209, 338, 850, 989
Hin6I GCGC 3 cut(s) 341, 466, 939
HinP1I GCGC 3 cut(s) 341, 466, 939
HincII GTYRAC 2 cut(s) 6, 863
HindII GTYRAC 2 cut(s) 6, 863
HinfI GANTC 6 cut(s) 71, 547, 570, 630, 998, 1023
HpaII CCGG 3 cut(s) 215, 461, 956
HphI GGTGA 1 cut(s) 570
Hpy166II GTNNAC 4 cut(s) 6, 665, 769, 863
Hpy188I TCNGA 5 cut(s) 297, 498, 594, 796, 1003
Hpy188III TCNNGA 3 cut(s) 565, 617, 674
Hpy8I GTNNAC 4 cut(s) 6, 665, 769, 863
Hpy99I CGWCG 2 cut(s) 10, 119
HpyAV CCTTC 4 cut(s) 55, 644, 715, 892
HpyCH4III ACNGT 3 cut(s) 253, 325, 896
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 8 cut(s) 329, 334, 445, 544, 611, 858, 876, 908
HpyF10VI GCNNNNNNNGC 3 cut(s) 211, 340, 463
HpyF3I CTNAG 7 cut(s) 57, 86, 369, 382, 558, 739, 1002
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 5 cut(s) 16, 209, 338, 850, 989
HspAI GCGC 3 cut(s) 341, 466, 939
Ksp22I TGATCA 2 cut(s) 208, 619
Kzo9I GATC 5 cut(s) 208, 363, 517, 619, 732
LmnI GCTCC 3 cut(s) 462, 463, 930
Lsp1109I GCAGC 4 cut(s) 206, 442, 455, 595
LweI GCATC 1 cut(s) 328
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 2 cut(s) 385, 713
MalI GATC 5 cut(s) 210, 365, 519, 621, 734
MboI GATC 5 cut(s) 208, 363, 517, 619, 732
MboII GAAGA 1 cut(s) 979
MflI RGATCY 2 cut(s) 363, 732
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 1 cut(s) 745
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 487
Mph1103I ATGCAT 1 cut(s) 336
MroXI GAANNNNTTC 1 cut(s) 574
MseI TTAA 3 cut(s) 282, 348, 839
MslI CAYNNNNRTG 1 cut(s) 675
MspI CCGG 3 cut(s) 215, 461, 956
MspR9I CCNGG 2 cut(s) 461, 956
MwoI GCNNNNNNNGC 3 cut(s) 211, 340, 463
NciI CCSGG 2 cut(s) 461, 956
NdeII GATC 5 cut(s) 208, 363, 517, 619, 732
NlaIII CATG 5 cut(s) 16, 209, 338, 850, 989
NlaIV GGNNCC 2 cut(s) 365, 898
NmuCI GTSAC 2 cut(s) 385, 713
NsiI ATGCAT 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 574
PfeI GAWTC 5 cut(s) 547, 570, 630, 998, 1023
PfoI TCCNGGA 1 cut(s) 459
PkrI GCNGC 4 cut(s) 196, 432, 470, 610
Ple19I CGATCG 1 cut(s) 520
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PsiI TTATAA 1 cut(s) 537
PspFI CCCAGC 1 cut(s) 396
PspN4I GGNNCC 2 cut(s) 365, 898
PsrI GAACNNNNNNTAC 2 cut(s) 962, 994
PsuI RGATCY 2 cut(s) 363, 732
PvuI CGATCG 1 cut(s) 520
RsaI GTAC 3 cut(s) 666, 770, 880
RsaNI GTAC 3 cut(s) 665, 769, 879
RseI CAYNNNNRTG 1 cut(s) 675
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 282, 348, 839
SatI GCNGC 4 cut(s) 195, 431, 469, 609
Sau3AI GATC 5 cut(s) 208, 363, 517, 619, 732
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 2 cut(s) 461, 956
SduI GDGCHC 1 cut(s) 227
SfaNI GCATC 1 cut(s) 328
SfcI CTRYAG 1 cut(s) 960
SmiMI CAYNNNNRTG 1 cut(s) 675
Sse9I AATT 1 cut(s) 745
SsiI CCGC 2 cut(s) 18, 142
StyD4I CCNGG 2 cut(s) 459, 954
StyI CCWWGG 2 cut(s) 99, 189
TaaI ACNGT 3 cut(s) 253, 325, 896
TaiI ACGT 1 cut(s) 11
TaqI TCGA 3 cut(s) 5, 48, 520
TasI AATT 1 cut(s) 745
TatI WGTACW 1 cut(s) 664
TfiI GAWTC 5 cut(s) 547, 570, 630, 998, 1023
Tru1I TTAA 3 cut(s) 282, 348, 839
Tru9I TTAA 3 cut(s) 282, 348, 839
TscAI CASTG 3 cut(s) 447, 489, 756
TseFI GTSAC 2 cut(s) 385, 713
TseI GCWGC 4 cut(s) 194, 430, 468, 608
Tsp45I GTSAC 2 cut(s) 385, 713
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 3 cut(s) 447, 489, 756
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 574
Zsp2I ATGCAT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.