FvH4_5g10180

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001035
Physical Location & Seq
Forward (+)
5817764 .. 5823345
5582 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10180.t9

Sequence Viewer

Length: 1029 bp
ATGTCGTTTTTCACGAATCACAATCCGAACAATTCCTTTGAGGTTGCTCAGCCTCCAACCGATTCCGTTTCAAGCCTCAGTTTCAGTCCTAAATCGAACCTTCTGGTCTCTACTTCCTGGGACAATCAGGTGCGGTGCTGGGATGTTCAAAAAATTGGAACAAGTATTGCTGCTACACCCAAGAGCTCCTATTCGCATGACCAGCCGGTTTTGTGCTCAATGTGGAAGGATGATGGGATGGCTGTGTTCTCTGGAGGTTGTGACAGGCAAGTTAAGATGTGGTCTTTAATGTCTGGAGGCCAACCGACTACTGTTGCTATGCATGATGCACCCATAAAAGAGATTGCTTGGATACCCGAGCTTGCGCTTTTAGTCGCAGGAAGCTGGGACAAGACTTTGAAGTATTGGGATTTGAGGCAGCCAAATCCGGTGCATACCCAACTACTTCCCGAACGCTGTTATGCACTAACAGTGAAATATCCTCTGATGGTTGTGGGAACAGCAGACAGAAATCTGATCATGTTTAACTTGCAGAATCCAAAGACTGAATTCAAGAGAATCATTTCACCCCTTAAGTATCAGACAAGGTGTGTTGCTGCATTTCCTGATCAGCAAGGTTTCTTGGTTGGTTCTATTGAAGGGAGGGTTGGAGTGCAGCATCTAAATGAAGGACAGGAAAGTAAAAACTTTACCTTCAAATGCCACAGAGAAAACAATGTTATTTACTCAGTCAACTCTATAAACTTCCATCCTGTGTATCACACATTTGCTACAGCTGGGTCTGATGGTTCTATCAGTTTTTGGGATAAGGACAGCAAACAGAGACTCAAGGCCATGTCAAAGTGCAATCAACCCATTCCTTGCAGTACTTTCAACAATGATGGTTCCATATTTGCATACTCCGTTTGCTATGACTGGCACAAGGGTGCAGGAAATCATAATCCTTCTACTGCAAAGACATCGATTTTCCTCCACTTGCCACAGGATGCTGATGTAAAACCCAAGCCAAAACCTGCTGGAAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

343

Amino Acids

38.28

Weight (kDa)

8.87

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 2 - 228 4.2e-28 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 2 - 103 1.9e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 2 - 72 3.8e-07 CDC20/Fizzy WD40 domain
WDR55 PF24796 3 - 150 7.4e-09 WDR55
WD40_WDHD1_1st PF24817 4 - 73 3.9e-08 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 4 - 158 2.4e-11 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 5 - 67 4.4e-06 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 6 - 72 3e-07 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 78 - 213 6.9e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 88 - 227 2.6e-12 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 93 - 224 3e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 170 - 226 7.9e-06 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 174 - 227 7.2e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1021
AciI CCGC 1 cut(s) 133
AcsI RAATTY 1 cut(s) 548
AfaI GTAC 1 cut(s) 868
AflII CTTAAG 1 cut(s) 572
AgsI TTSAA 7 cut(s) 72, 149, 400, 553, 638, 697, 874
AjnI CCWGG 1 cut(s) 116
AjuI GAANNNNNNNTTGG 2 cut(s) 630, 662
AleI CACNNNNGTG 1 cut(s) 924
AluBI AGCT 4 cut(s) 186, 361, 384, 776
AluI AGCT 4 cut(s) 186, 361, 384, 776
Alw21I GWGCWC 2 cut(s) 188, 218
Alw26I GTCTC 2 cut(s) 112, 817
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 2 cut(s) 298, 831
ApeKI GCWGC 4 cut(s) 170, 418, 596, 655
ApoI RAATTY 1 cut(s) 548
Asp700I GAANNNNTTC 1 cut(s) 562
AspLEI GCGC 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 558
AvaI CYCGRG 1 cut(s) 356
BanII GRGCYC 1 cut(s) 188
Bbv12I GWGCWC 2 cut(s) 188, 218
BbvI GCAGC 4 cut(s) 157, 430, 583, 667
BccI CCATC 6 cut(s) 227, 232, 481, 756, 779, 875
BcgI CGANNNNNNTGC 2 cut(s) 942, 976
BciT130I CCWGG 1 cut(s) 118
BciVI GTATCC 1 cut(s) 345
BclI TGATCA 2 cut(s) 516, 607
BcoDI GTCTC 2 cut(s) 112, 817
BfmI CTRYAG 1 cut(s) 771
BfrI CTTAAG 1 cut(s) 572
BfuAI ACCTGC 1 cut(s) 1021
BfuI GTATCC 1 cut(s) 345
BisI GCNGC 4 cut(s) 171, 419, 597, 656
BlpI GCTNAGC 1 cut(s) 48
BlsI GCNGC 4 cut(s) 172, 420, 598, 657
BmcAI AGTACT 1 cut(s) 868
Bme1390I CCNGG 1 cut(s) 118
BmeT110I CYCGRG 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 886
BmrFI CCNGG 1 cut(s) 118
BmsI GCATC 3 cut(s) 316, 667, 976
BpmI CTGGAG 2 cut(s) 273, 315
Bpu1102I GCTNAGC 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 812
Bsa29I ATCGAT 1 cut(s) 962
BsaI GGTCTC 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 117
BsaWI WCCGGW 1 cut(s) 427
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 1 cut(s) 920
BseBI CCWGG 1 cut(s) 118
BseCI ATCGAT 1 cut(s) 962
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 5 cut(s) 148, 235, 243, 748, 991
BseMII CTCAG 3 cut(s) 62, 91, 741
BseNI ACTGG 1 cut(s) 920
BseXI GCAGC 4 cut(s) 157, 430, 583, 667
BseYI CCCAGC 3 cut(s) 138, 384, 776
BsgI GTGCAG 2 cut(s) 674, 948
BshFI GGCC 2 cut(s) 300, 833
BshVI ATCGAT 1 cut(s) 962
BsiHKAI GWGCWC 2 cut(s) 188, 218
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 2 cut(s) 206, 428
BslFI GGGAC 2 cut(s) 134, 401
BsmAI GTCTC 2 cut(s) 112, 817
BsmFI GGGAC 2 cut(s) 134, 401
BsnI GGCC 2 cut(s) 300, 833
Bso31I GGTCTC 1 cut(s) 112
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 2 cut(s) 188, 218
Bsp143I GATC 2 cut(s) 516, 607
Bsp1720I GCTNAGC 1 cut(s) 48
BspACI CCGC 1 cut(s) 133
BspANI GGCC 2 cut(s) 300, 833
BspCNI CTCAG 3 cut(s) 61, 90, 740
BspDI ATCGAT 1 cut(s) 962
BspLI GGNNCC 1 cut(s) 886
BspMI ACCTGC 1 cut(s) 1021
BspTI CTTAAG 1 cut(s) 572
BspTNI GGTCTC 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 205
BsrI ACTGG 1 cut(s) 920
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 2 cut(s) 516, 607
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 2 cut(s) 313, 472
BstAFI CTTAAG 1 cut(s) 572
BstC8I GCNNGC 1 cut(s) 363
BstDEI CTNAG 3 cut(s) 48, 77, 727
BstF5I GGATG 5 cut(s) 148, 235, 243, 748, 991
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 2 cut(s) 519, 610
BstMAI GTCTC 2 cut(s) 112, 817
BstMBI GATC 2 cut(s) 516, 607
BstMWI GCNNNNNNNGC 1 cut(s) 202
BstNI CCWGG 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 116
BstSFI CTRYAG 1 cut(s) 771
BstV1I GCAGC 4 cut(s) 157, 430, 583, 667
Bsu15I ATCGAT 1 cut(s) 962
BsuI GTATCC 1 cut(s) 345
BsuRI GGCC 2 cut(s) 300, 833
BsuTUI ATCGAT 1 cut(s) 962
BtsCI GGATG 5 cut(s) 148, 235, 243, 748, 991
BtsIMutI CAGTG 1 cut(s) 477
BveI ACCTGC 1 cut(s) 1021
Cac8I GCNNGC 1 cut(s) 363
CfoI GCGC 1 cut(s) 367
Cfr10I RCCGGY 1 cut(s) 205
ClaI ATCGAT 1 cut(s) 962
Csp6I GTAC 1 cut(s) 867
CviAII CATG 4 cut(s) 197, 323, 520, 835
CviQI GTAC 1 cut(s) 867
DdeI CTNAG 3 cut(s) 48, 77, 727
DpnI GATC 2 cut(s) 518, 609
DpnII GATC 2 cut(s) 516, 607
Ecl136II GAGCTC 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 186
Eco88I CYCGRG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 186
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 116
EcoT22I ATGCAT 1 cut(s) 324
EcoT38I GRGCYC 1 cut(s) 188
FaeI CATG 4 cut(s) 200, 326, 523, 838
FaqI GGGAC 2 cut(s) 134, 401
FatI CATG 4 cut(s) 196, 322, 519, 834
FbaI TGATCA 2 cut(s) 516, 607
Fnu4HI GCNGC 4 cut(s) 171, 419, 597, 656
FokI GGATG 5 cut(s) 155, 242, 250, 735, 998
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 4 cut(s) 171, 419, 597, 656
GlaI GCGC 1 cut(s) 366
GluI GCNGC 4 cut(s) 171, 419, 597, 656
GsaI CCCAGC 3 cut(s) 142, 388, 780
GsuI CTGGAG 2 cut(s) 273, 315
HaeIII GGCC 2 cut(s) 300, 833
HapII CCGG 2 cut(s) 206, 428
HhaI GCGC 1 cut(s) 367
Hin1II CATG 4 cut(s) 200, 326, 523, 838
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HinfI GANTC 5 cut(s) 16, 62, 535, 558, 825
HpaII CCGG 2 cut(s) 206, 428
HphI GGTGA 1 cut(s) 558
Hpy166II GTNNAC 1 cut(s) 733
Hpy188I TCNGA 5 cut(s) 27, 486, 516, 582, 784
Hpy188III TCNNGA 6 cut(s) 13, 252, 294, 449, 553, 605
Hpy8I GTNNAC 1 cut(s) 733
HpyAV CCTTC 7 cut(s) 110, 220, 632, 662, 703, 954, 1017
HpyCH4III ACNGT 2 cut(s) 313, 472
HpyF10VI GCNNNNNNNGC 1 cut(s) 202
HpyF3I CTNAG 3 cut(s) 48, 77, 727
Hsp92II CATG 4 cut(s) 200, 326, 523, 838
HspAI GCGC 1 cut(s) 365
Ksp22I TGATCA 2 cut(s) 516, 607
Kzo9I GATC 2 cut(s) 516, 607
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 4 cut(s) 157, 430, 583, 667
LweI GCATC 3 cut(s) 316, 667, 976
MaeIII GTNAC 1 cut(s) 260
MalI GATC 2 cut(s) 518, 609
MboI GATC 2 cut(s) 516, 607
MhlI GDGCHC 2 cut(s) 188, 218
MluCI AATT 3 cut(s) 31, 153, 548
MlyI GAGTC 1 cut(s) 819
MmeI TCCRAC 2 cut(s) 80, 628
MnlI CCTC 9 cut(s) 34, 63, 86, 248, 290, 408, 492, 636, 980
Mph1103I ATGCAT 1 cut(s) 324
MroXI GAANNNNTTC 1 cut(s) 562
MseI TTAA 4 cut(s) 273, 287, 525, 573
MslI CAYNNNNRTG 2 cut(s) 663, 924
MspA1I CMGCKG 1 cut(s) 776
MspCI CTTAAG 1 cut(s) 572
MspI CCGG 2 cut(s) 206, 428
MspR9I CCNGG 1 cut(s) 118
MvaI CCWGG 1 cut(s) 118
MwoI GCNNNNNNNGC 1 cut(s) 202
NdeII GATC 2 cut(s) 516, 607
NlaIII CATG 4 cut(s) 200, 326, 523, 838
NlaIV GGNNCC 1 cut(s) 886
NmuCI GTSAC 1 cut(s) 260
NsiI ATGCAT 1 cut(s) 324
OliI CACNNNNGTG 1 cut(s) 924
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PdmI GAANNNNTTC 1 cut(s) 562
PfeI GAWTC 4 cut(s) 16, 62, 535, 558
PkrI GCNGC 4 cut(s) 172, 420, 598, 657
PleI GAGTC 1 cut(s) 819
PpsI GAGTC 1 cut(s) 819
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 116
PspFI CCCAGC 3 cut(s) 138, 384, 776
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 886
PvuII CAGCTG 1 cut(s) 776
RsaI GTAC 1 cut(s) 868
RsaNI GTAC 1 cut(s) 867
RseI CAYNNNNRTG 2 cut(s) 663, 924
SacI GAGCTC 1 cut(s) 188
SaqAI TTAA 4 cut(s) 273, 287, 525, 573
SatI GCNGC 4 cut(s) 171, 419, 597, 656
Sau3AI GATC 2 cut(s) 516, 607
ScaI AGTACT 1 cut(s) 868
SchI GAGTC 1 cut(s) 819
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 2 cut(s) 188, 218
SfaNI GCATC 3 cut(s) 316, 667, 976
SfcI CTRYAG 1 cut(s) 771
SmiMI CAYNNNNRTG 2 cut(s) 663, 924
SmlI CTYRAG 2 cut(s) 572, 827
SmoI CTYRAG 2 cut(s) 572, 827
Sse9I AATT 3 cut(s) 31, 153, 548
SsiI CCGC 1 cut(s) 133
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 1 cut(s) 116
TaaI ACNGT 2 cut(s) 313, 472
TaqI TCGA 2 cut(s) 95, 962
TasI AATT 3 cut(s) 31, 153, 548
TatI WGTACW 1 cut(s) 866
TfiI GAWTC 4 cut(s) 16, 62, 535, 558
Tru1I TTAA 4 cut(s) 273, 287, 525, 573
Tru9I TTAA 4 cut(s) 273, 287, 525, 573
TscAI CASTG 1 cut(s) 477
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 4 cut(s) 170, 418, 596, 655
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 1 cut(s) 681
TspGWI ACGGA 2 cut(s) 55, 892
TspRI CASTG 1 cut(s) 477
Vha464I CTTAAG 1 cut(s) 572
XapI RAATTY 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 562
ZrmI AGTACT 1 cut(s) 868
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.