Rh7CG387700

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001035
Physical Location & Seq
Forward (+)
49001352 .. 49009120
7769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG387700.1

Sequence Viewer

Length: 1047 bp
ATGTCGACGTTCATGGCCGCTTCCAATCCAAATCCAAACAAATCCTTCGAGGTCGCTCAGCCTCCCACCGACTCCGTTTCGAGTCTCAGCTTCAGCCCTAAGGCTAACTTTCTGGTCGCAACCTCCTGGGACAACCAGGTGCGATGTTGGGAGATACAACAGAGTGGAGGAAATGTTGCCAGTATGCCCAAGGCAGCTATATCGCACGATAATCCGGTTTTGTGCTCAACTTGGAAAGATGATGGGACAACTGTGTTCTCTGGAGGTTGTGATAAGCAAGTTAAGATGTGGCCTCTGATGTCTGGTGGCAACCAAACAATGACTGTTGCAATGCATGATGCGCCCATCAAGGAAATAGCTTGGATCCCTGAGATGAGCCTCTTGGTGACAGGAAGCTGGGACAAGACTTTGAAGTATTGGGATACAAGGCAGCCAAATCCAGTGCATACCCAACTGCTCCCAGAGAGGTGCTATGCATTATCAGTGAGACACCCTCTGATGGTGGTTGGAACTGCTGATCGAAATCTTATTGTTTATAACTTGCAGAGTCCACAGACTGAGTTCAAGAGAATCATTTCACCCCTAAAGTATCAGACAAGGTGTGTTGCGGCATTTCCTGATCAGCAAGGATTCTTGGTTGGCTCTATTGAAGGGAGGGTTGGTGTACATCATCTGGATGAGGCACAACAAAGTAAAAACTTTACCTTCAAATGCCACAGAGAGGGCAACGAGATATACTCAGTCAATTCTCTGAACTTCCATCCTGTGTATCACACATTTGCTACTGCTGGTTCTGATGGTTCTTTCAACTTTTGGGATAAGGACAGCAAACAGAGACTAAAGGCCATGGCAAGGTGCAGTCAACCAATACCTTGCAGCACTTTCAACAACGATGGTTCCATATTTGCATACTCGGTTTGCTATGATTGGAGTAAGGGCGCAGAAAATCACAATCCGGCCACAGCAAAGAACTACATTTACCTCCATGTTCCACAGGAATCTGAGGTGAAAAGCAAGCCACGAATCTCGACTGGTGGAAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

348

Amino Acids

38.85

Weight (kDa)

8.49

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 14 - 149 2.4e-13 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 20 - 171 1.2e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 20 - 181 7.9e-19 WDR3 first beta-propeller domain
WD40 PF00400 23 - 50 6.1e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 23 - 142 1.6e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 23 - 233 6e-21 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 24 - 179 8.8e-12 WDR3 second beta-propeller domain
WDR55 PF24796 24 - 232 2e-17 WDR55
WD40_Gbeta PF25391 26 - 145 3.3e-09 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 68 - 148 2e-08 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 69 - 155 4.2e-10 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 69 - 305 7.9e-30 THOC3 beta-propeller domain
WD40 PF00400 105 - 141 8.7e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 120 - 234 3.2e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 155 - 285 1.6e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 163 - 304 7.4e-13 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 165 - 308 2e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 169 - 301 8.6e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 537
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 18, 608
AclWI GGATC 2 cut(s) 358, 371
AcoI YGGCCR 2 cut(s) 15, 957
AcuI CTGAAG 1 cut(s) 76
AfaI GTAC 1 cut(s) 666
AfiI CCNNNNNNNGG 3 cut(s) 499, 721, 852
AgsI TTSAA 6 cut(s) 412, 565, 650, 709, 808, 886
AjnI CCWGG 2 cut(s) 125, 135
AjuI GAANNNNNNNTTGG 2 cut(s) 642, 674
AluBI AGCT 4 cut(s) 90, 197, 359, 396
AluI AGCT 4 cut(s) 90, 197, 359, 396
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 3 cut(s) 89, 481, 829
AlwI GGATC 2 cut(s) 358, 371
AoxI GGCC 4 cut(s) 15, 290, 843, 957
ApeKI GCWGC 3 cut(s) 194, 430, 876
Asp700I GAANNNNTTC 1 cut(s) 574
AspLEI GCGC 2 cut(s) 343, 941
AsuHPI GGTGA 3 cut(s) 397, 570, 1018
AxyI CCTNAGG 1 cut(s) 99
BamHI GGATCC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 3 cut(s) 206, 442, 888
BccI CCATC 6 cut(s) 236, 353, 493, 768, 791, 887
BcgI CGANNNNNNTGC 2 cut(s) 183, 217
BciT130I CCWGG 2 cut(s) 127, 137
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 3 cut(s) 89, 481, 829
BfuI GTATCC 1 cut(s) 415
BisI GCNGC 5 cut(s) 18, 195, 431, 609, 877
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 5 cut(s) 19, 196, 432, 610, 878
Bme1390I CCNGG 2 cut(s) 127, 137
BmiI GGNNCC 2 cut(s) 365, 898
BmrFI CCNGG 2 cut(s) 127, 137
BmsI GCATC 1 cut(s) 328
BplI GAGNNNNNCTC 4 cut(s) 478, 510, 722, 754
BpmI CTGGAG 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 57
BsaBI GATNNNNATC 1 cut(s) 522
BsaJI CCNNGG 3 cut(s) 126, 189, 846
BsaWI WCCGGW 1 cut(s) 214
BsaXI ACNNNNNCTCC 4 cut(s) 159, 189, 646, 676
Bsc4I CCNNNNNNNGG 3 cut(s) 499, 721, 852
Bse1I ACTGG 3 cut(s) 180, 440, 1036
Bse21I CCTNAGG 1 cut(s) 99
Bse3DI GCAATG 1 cut(s) 336
Bse8I GATNNNNATC 1 cut(s) 522
BseBI CCWGG 2 cut(s) 127, 137
BseDI CCNNGG 3 cut(s) 126, 189, 846
BseGI GGATG 2 cut(s) 682, 760
BseJI GATNNNNATC 1 cut(s) 522
BseLI CCNNNNNNNGG 3 cut(s) 499, 721, 852
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 6 cut(s) 71, 100, 360, 549, 753, 993
BseNI ACTGG 3 cut(s) 180, 440, 1036
BseXI GCAGC 3 cut(s) 206, 442, 888
BseYI CCCAGC 1 cut(s) 396
BsgI GTGCAG 1 cut(s) 877
BshFI GGCC 4 cut(s) 17, 292, 845, 959
BsiHKAI GWGCWC 1 cut(s) 227
BsiSI CCGG 2 cut(s) 215, 956
BslFI GGGAC 3 cut(s) 143, 259, 413
BslI CCNNNNNNNGG 3 cut(s) 499, 721, 852
BsmAI GTCTC 3 cut(s) 89, 481, 829
BsmFI GGGAC 3 cut(s) 143, 259, 413
BsnI GGCC 4 cut(s) 17, 292, 845, 959
Bsp1286I GDGCHC 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 664
Bsp143I GATC 3 cut(s) 363, 517, 619
Bsp1720I GCTNAGC 1 cut(s) 57
Bsp19I CCATGG 1 cut(s) 846
BspACI CCGC 2 cut(s) 18, 608
BspANI GGCC 4 cut(s) 17, 292, 845, 959
BspCNI CTCAG 6 cut(s) 70, 99, 361, 550, 752, 994
BspLI GGNNCC 2 cut(s) 365, 898
BspPI GGATC 2 cut(s) 358, 371
BsrDI GCAATG 1 cut(s) 336
BsrGI TGTACA 1 cut(s) 664
BsrI ACTGG 3 cut(s) 180, 440, 1036
BssECI CCNNGG 3 cut(s) 126, 189, 846
BssMI GATC 3 cut(s) 363, 517, 619
BssT1I CCWWGG 2 cut(s) 189, 846
Bst2UI CCWGG 2 cut(s) 127, 137
Bst4CI ACNGT 2 cut(s) 253, 325
BstAUI TGTACA 1 cut(s) 664
BstC8I GCNNGC 1 cut(s) 1016
BstDEI CTNAG 7 cut(s) 57, 86, 99, 369, 558, 739, 1002
BstDSI CCRYGG 1 cut(s) 846
BstF5I GGATG 2 cut(s) 682, 760
BstHHI GCGC 2 cut(s) 343, 941
BstKTI GATC 3 cut(s) 366, 520, 622
BstMAI GTCTC 3 cut(s) 89, 481, 829
BstMBI GATC 3 cut(s) 363, 517, 619
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstNI CCWGG 2 cut(s) 127, 137
BstSCI CCNGG 2 cut(s) 125, 135
BstV1I GCAGC 3 cut(s) 206, 442, 888
BstX2I RGATCY 1 cut(s) 363
BstYI RGATCY 1 cut(s) 363
Bsu36I CCTNAGG 1 cut(s) 99
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 4 cut(s) 17, 292, 845, 959
BtgI CCRYGG 1 cut(s) 846
BtgZI GCGATG 1 cut(s) 157
BtsCI GGATG 2 cut(s) 682, 760
BtsIMutI CAGTG 2 cut(s) 447, 489
Cac8I GCNNGC 1 cut(s) 1016
CfoI GCGC 2 cut(s) 343, 941
CsiI ACCWGGT 1 cut(s) 135
Csp6I GTAC 1 cut(s) 665
CviAII CATG 4 cut(s) 13, 335, 847, 986
CviQI GTAC 1 cut(s) 665
DdeI CTNAG 7 cut(s) 57, 86, 99, 369, 558, 739, 1002
DpnI GATC 3 cut(s) 365, 519, 621
DpnII GATC 3 cut(s) 363, 517, 619
EaeI YGGCCR 2 cut(s) 15, 957
Eco130I CCWWGG 2 cut(s) 189, 846
Eco57I CTGAAG 1 cut(s) 76
Eco81I CCTNAGG 1 cut(s) 99
EcoRII CCWGG 2 cut(s) 125, 135
EcoT14I CCWWGG 2 cut(s) 189, 846
EcoT22I ATGCAT 2 cut(s) 336, 478
ErhI CCWWGG 2 cut(s) 189, 846
FaeI CATG 4 cut(s) 16, 338, 850, 989
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FaqI GGGAC 3 cut(s) 143, 259, 413
FatI CATG 4 cut(s) 12, 334, 846, 985
FbaI TGATCA 1 cut(s) 619
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 5 cut(s) 18, 195, 431, 609, 877
FokI GGATG 2 cut(s) 689, 747
Fsp4HI GCNGC 5 cut(s) 18, 195, 431, 609, 877
GlaI GCGC 2 cut(s) 342, 940
GluI GCNGC 5 cut(s) 18, 195, 431, 609, 877
GsaI CCCAGC 1 cut(s) 400
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 4 cut(s) 17, 292, 845, 959
HapII CCGG 2 cut(s) 215, 956
HhaI GCGC 2 cut(s) 343, 941
Hin1II CATG 4 cut(s) 16, 338, 850, 989
Hin6I GCGC 2 cut(s) 341, 939
HinP1I GCGC 2 cut(s) 341, 939
HincII GTYRAC 2 cut(s) 6, 863
HindII GTYRAC 2 cut(s) 6, 863
HinfI GANTC 7 cut(s) 71, 82, 547, 570, 630, 998, 1023
HpaII CCGG 2 cut(s) 215, 956
HphI GGTGA 3 cut(s) 397, 570, 1018
Hpy166II GTNNAC 4 cut(s) 6, 551, 665, 863
Hpy188I TCNGA 6 cut(s) 297, 498, 594, 753, 796, 1003
Hpy188III TCNNGA 5 cut(s) 261, 565, 617, 674, 1027
Hpy8I GTNNAC 4 cut(s) 6, 551, 665, 863
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 55, 644, 715
HpyCH4III ACNGT 2 cut(s) 253, 325
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 8 cut(s) 329, 334, 445, 476, 544, 858, 876, 908
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpyF3I CTNAG 7 cut(s) 57, 86, 99, 369, 558, 739, 1002
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 4 cut(s) 16, 338, 850, 989
HspAI GCGC 2 cut(s) 341, 939
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 3 cut(s) 363, 517, 619
LmnI GCTCC 1 cut(s) 462
Lsp1109I GCAGC 3 cut(s) 206, 442, 888
LweI GCATC 1 cut(s) 328
MabI ACCWGGT 1 cut(s) 135
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 1 cut(s) 385
MalI GATC 3 cut(s) 365, 519, 621
MboI GATC 3 cut(s) 363, 517, 619
MflI RGATCY 1 cut(s) 363
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 1 cut(s) 745
MlyI GAGTC 3 cut(s) 65, 91, 556
MmeI TCCRAC 1 cut(s) 487
Mph1103I ATGCAT 2 cut(s) 336, 478
MroXI GAANNNNTTC 1 cut(s) 574
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 675
MspI CCGG 2 cut(s) 215, 956
MspR9I CCNGG 2 cut(s) 127, 137
MvaI CCWGG 2 cut(s) 127, 137
MwoI GCNNNNNNNGC 1 cut(s) 340
NcoI CCATGG 1 cut(s) 846
NdeII GATC 3 cut(s) 363, 517, 619
NlaIII CATG 4 cut(s) 16, 338, 850, 989
NlaIV GGNNCC 2 cut(s) 365, 898
NmuCI GTSAC 1 cut(s) 385
NsiI ATGCAT 2 cut(s) 336, 478
PdmI GAANNNNTTC 1 cut(s) 574
PfeI GAWTC 4 cut(s) 570, 630, 998, 1023
PkrI GCNGC 5 cut(s) 19, 196, 432, 610, 878
PleI GAGTC 3 cut(s) 65, 90, 555
PpsI GAGTC 3 cut(s) 65, 90, 555
PsiI TTATAA 1 cut(s) 537
Psp6I CCWGG 2 cut(s) 125, 135
PspFI CCCAGC 1 cut(s) 396
PspGI CCWGG 2 cut(s) 125, 135
PspN4I GGNNCC 2 cut(s) 365, 898
PsrI GAACNNNNNNTAC 2 cut(s) 962, 994
PsuI RGATCY 1 cut(s) 363
RsaI GTAC 1 cut(s) 666
RsaNI GTAC 1 cut(s) 665
RseI CAYNNNNRTG 1 cut(s) 675
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 5 cut(s) 18, 195, 431, 609, 877
Sau3AI GATC 3 cut(s) 363, 517, 619
SchI GAGTC 3 cut(s) 65, 91, 556
ScrFI CCNGG 2 cut(s) 127, 137
SduI GDGCHC 1 cut(s) 227
SexAI ACCWGGT 1 cut(s) 135
SfaNI GCATC 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 675
Sse9I AATT 1 cut(s) 745
SsiI CCGC 2 cut(s) 18, 608
StyD4I CCNGG 2 cut(s) 125, 135
StyI CCWWGG 2 cut(s) 189, 846
TaaI ACNGT 2 cut(s) 253, 325
TaiI ACGT 1 cut(s) 11
TaqI TCGA 5 cut(s) 5, 48, 80, 520, 1028
TasI AATT 1 cut(s) 745
TatI WGTACW 1 cut(s) 664
TauI GCSGC 2 cut(s) 20, 611
TfiI GAWTC 4 cut(s) 570, 630, 998, 1023
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 2 cut(s) 447, 489
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 3 cut(s) 194, 430, 876
Tsp45I GTSAC 1 cut(s) 385
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 2 cut(s) 447, 489
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 574
Zsp2I ATGCAT 2 cut(s) 336, 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.