Rmu_ssc0000422.1_g000019

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000422.1
OG0001035
Physical Location & Seq
Reverse (-)
83473 .. 88859
5387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000422.1_g000019.1.cds

Sequence Viewer

Length: 1182 bp
atgtcggcttttatgaatcacaatccgaacaattcctttgaggtcgctcagcctccaagcgattctgtttcaagcctcagtttcagtcccaaatcgaaccttctggtctctacctcctgggacaatcaggtgcgatgctgggatattcaaagaattggaacaaatattgctgctgcgccgaagagctccttttcgcatgaccatccggttttgtgctcaacatggaaggatgatgggatggctgtattctctggaggttgtgacaggcaagttaagatgtggtctctaatgtctggtggccaaacgagtactgttgccatgcatgatgcacccataaaagagattgcttggattcccgagatggcgctcttagtcacaggcagctgggacaagactatgaagtactgggatttaaggcagccaaatccagtgcatacccaacaactacccgaacgctgttatgcactaacagtgaaacaccctctaatggttgttggaacagcagatagaaatctgatcatatttaacttgcagagtccacagactgagttcaagagaatcatttcacccctgaagtatcagacaaggtgtgttgctgcatttcctgataagcaaggtttcttggttggttcaattgaagggagggttggagtgcagcatctggatgaaggacaagatagtaaaaactttaccttcaaatgccacagagaaaacaatgctctttactcagtcaactctataaacttccatcctgtgcatcacacattttctacagctggttctgatggttctatcagtttttgggataaggacagcaaacagagactcaaggccatgcaaaggtgcaatcaacccattccttgcagtactttcaacaatgatggttccatatatgcgtactcggtttgctatgactggcataagggagcagaaaatcataatccttctactgcaaagacctccattttcctccacttgccacaggacgctgatgttaaaagcaagccaagacctggtggaagaagaattttgttgaaggctgccctaaccaaaatcattgattataggagcatgggctttgtcccatgcaagtccccactcctagccttctatgcatctttgaactttaattctgattgtgaagttgcctctgacgctggcaacttcggtttgaatagttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

393

Amino Acids

43.8

Weight (kDa)

8.56

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1013
AcoI YGGCCR 1 cut(s) 298
AcsI RAATTY 1 cut(s) 1026
AcuI CTGAAG 1 cut(s) 593
AfaI GTAC 4 cut(s) 310, 404, 868, 899
AfiI CCNNNNNNNGG 3 cut(s) 487, 840, 1013
AjnI CCWGG 2 cut(s) 116, 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 630, 662
AluBI AGCT 3 cut(s) 186, 384, 776
AluI AGCT 3 cut(s) 186, 384, 776
Alw21I GWGCWC 2 cut(s) 188, 218
Alw26I GTCTC 3 cut(s) 112, 288, 817
AlwNI CAGNNNCTG 1 cut(s) 661
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 2 cut(s) 298, 831
ApeKI GCWGC 7 cut(s) 170, 173, 381, 418, 596, 655, 1040
ApoI RAATTY 1 cut(s) 1026
Asp700I GAANNNNTTC 1 cut(s) 562
AspLEI GCGC 2 cut(s) 178, 367
AsuHPI GGTGA 1 cut(s) 558
AvaI CYCGRG 1 cut(s) 356
BalI TGGCCA 1 cut(s) 300
BanII GRGCYC 1 cut(s) 188
Bbv12I GWGCWC 2 cut(s) 188, 218
BbvI GCAGC 7 cut(s) 157, 160, 393, 430, 583, 667, 1027
BccI CCATC 7 cut(s) 210, 227, 232, 355, 756, 779, 875
BciT130I CCWGG 2 cut(s) 118, 1014
BclI TGATCA 1 cut(s) 516
BcoDI GTCTC 3 cut(s) 112, 288, 817
BfaI CTAG 1 cut(s) 1103
BfmI CTRYAG 1 cut(s) 771
BfoI RGCGCY 1 cut(s) 368
BisI GCNGC 7 cut(s) 171, 174, 382, 419, 597, 656, 1041
BlpI GCTNAGC 1 cut(s) 48
BlsI GCNGC 7 cut(s) 172, 175, 383, 420, 598, 657, 1042
BmcAI AGTACT 3 cut(s) 310, 404, 868
Bme1390I CCNGG 2 cut(s) 118, 1014
BmeT110I CYCGRG 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 886
BmrFI CCNGG 2 cut(s) 118, 1014
BmrI ACTGGG 1 cut(s) 415
BmsI GCATC 5 cut(s) 125, 316, 667, 766, 1124
BmuI ACTGGG 1 cut(s) 415
BpmI CTGGAG 1 cut(s) 273
Bpu1102I GCTNAGC 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 812
BsaBI GATNNNNATC 1 cut(s) 510
BsaI GGTCTC 2 cut(s) 112, 288
BsaJI CCNNGG 1 cut(s) 117
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 3 cut(s) 487, 840, 1013
Bse1I ACTGG 3 cut(s) 410, 428, 920
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 2 cut(s) 118, 1014
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 5 cut(s) 202, 235, 243, 670, 748
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 3 cut(s) 487, 840, 1013
BseMII CTCAG 4 cut(s) 62, 91, 537, 741
BseNI ACTGG 3 cut(s) 410, 428, 920
BseXI GCAGC 7 cut(s) 157, 160, 393, 430, 583, 667, 1027
BseYI CCCAGC 2 cut(s) 138, 384
BsgI GTGCAG 1 cut(s) 674
BshFI GGCC 2 cut(s) 300, 833
BsiHKAI GWGCWC 2 cut(s) 188, 218
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 5 cut(s) 72, 134, 401, 1067, 1078
BslI CCNNNNNNNGG 3 cut(s) 487, 840, 1013
BsmAI GTCTC 3 cut(s) 112, 288, 817
BsmFI GGGAC 5 cut(s) 72, 134, 401, 1067, 1078
BsnI GGCC 2 cut(s) 300, 833
Bso31I GGTCTC 2 cut(s) 112, 288
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 2 cut(s) 188, 218
Bsp143I GATC 1 cut(s) 516
Bsp1720I GCTNAGC 1 cut(s) 48
BspANI GGCC 2 cut(s) 300, 833
BspCNI CTCAG 4 cut(s) 61, 90, 538, 740
BspLI GGNNCC 1 cut(s) 886
BspQI GCTCTTC 1 cut(s) 176
BspTNI GGTCTC 2 cut(s) 112, 288
BsrI ACTGG 3 cut(s) 410, 428, 920
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 1 cut(s) 516
Bst2UI CCWGG 2 cut(s) 118, 1014
Bst4CI ACNGT 2 cut(s) 313, 472
Bst6I CTCTTC 1 cut(s) 176
BstC8I GCNNGC 2 cut(s) 1004, 1159
BstDEI CTNAG 5 cut(s) 48, 77, 370, 546, 727
BstF5I GGATG 5 cut(s) 202, 235, 243, 670, 748
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 2 cut(s) 178, 367
BstKTI GATC 1 cut(s) 519
BstMAI GTCTC 3 cut(s) 112, 288, 817
BstMBI GATC 1 cut(s) 516
BstMWI GCNNNNNNNGC 2 cut(s) 1112, 1154
BstNI CCWGG 2 cut(s) 118, 1014
BstSCI CCNGG 2 cut(s) 116, 1012
BstSFI CTRYAG 1 cut(s) 771
BstV1I GCAGC 7 cut(s) 157, 160, 393, 430, 583, 667, 1027
BsuRI GGCC 2 cut(s) 300, 833
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 5 cut(s) 202, 235, 243, 670, 748
BtsIMutI CAGTG 2 cut(s) 435, 477
Cac8I GCNNGC 2 cut(s) 1004, 1159
CaiI CAGNNNCTG 1 cut(s) 661
CfoI GCGC 2 cut(s) 178, 367
CseI GACGC 2 cut(s) 995, 1163
CsiI ACCWGGT 1 cut(s) 1012
Csp6I GTAC 4 cut(s) 309, 403, 867, 898
CviAII CATG 7 cut(s) 197, 222, 319, 323, 835, 1072, 1086
CviQI GTAC 4 cut(s) 309, 403, 867, 898
DdeI CTNAG 5 cut(s) 48, 77, 370, 546, 727
DpnI GATC 1 cut(s) 518
DpnII GATC 1 cut(s) 516
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 2 cut(s) 112, 288
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 116, 1012
EcoT22I ATGCAT 2 cut(s) 324, 1117
EcoT38I GRGCYC 1 cut(s) 188
FaeI CATG 7 cut(s) 200, 225, 322, 326, 838, 1075, 1089
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 5 cut(s) 72, 134, 401, 1067, 1078
FatI CATG 7 cut(s) 196, 221, 318, 322, 834, 1071, 1085
FbaI TGATCA 1 cut(s) 516
Fnu4HI GCNGC 7 cut(s) 171, 174, 382, 419, 597, 656, 1041
FokI GGATG 5 cut(s) 189, 242, 250, 677, 735
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 7 cut(s) 171, 174, 382, 419, 597, 656, 1041
FspBI CTAG 1 cut(s) 1103
GlaI GCGC 2 cut(s) 177, 366
GluI GCNGC 7 cut(s) 171, 174, 382, 419, 597, 656, 1041
GsaI CCCAGC 2 cut(s) 142, 388
GsuI CTGGAG 1 cut(s) 273
HaeII RGCGCY 1 cut(s) 368
HaeIII GGCC 2 cut(s) 300, 833
HapII CCGG 1 cut(s) 206
HgaI GACGC 2 cut(s) 995, 1163
HhaI GCGC 2 cut(s) 178, 367
Hin1II CATG 7 cut(s) 200, 225, 322, 326, 838, 1075, 1089
Hin6I GCGC 2 cut(s) 176, 365
HinP1I GCGC 2 cut(s) 176, 365
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HinfI GANTC 6 cut(s) 16, 62, 352, 535, 558, 825
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 558
Hpy166II GTNNAC 2 cut(s) 539, 733
Hpy188I TCNGA 6 cut(s) 27, 516, 582, 784, 1135, 1153
Hpy188III TCNNGA 5 cut(s) 252, 356, 553, 605, 662
Hpy8I GTNNAC 2 cut(s) 539, 733
HpyAV CCTTC 8 cut(s) 110, 220, 632, 662, 703, 954, 1030, 1117
HpyCH4III ACNGT 2 cut(s) 313, 472
HpyF10VI GCNNNNNNNGC 2 cut(s) 1112, 1154
HpyF3I CTNAG 5 cut(s) 48, 77, 370, 546, 727
Hsp92II CATG 7 cut(s) 200, 225, 322, 326, 838, 1075, 1089
HspAI GCGC 2 cut(s) 176, 365
Ksp22I TGATCA 1 cut(s) 516
Kzo9I GATC 1 cut(s) 516
LguI GCTCTTC 1 cut(s) 176
LmnI GCTCC 3 cut(s) 191, 926, 1068
Lsp1109I GCAGC 7 cut(s) 157, 160, 393, 430, 583, 667, 1027
LweI GCATC 5 cut(s) 125, 316, 667, 766, 1124
MabI ACCWGGT 1 cut(s) 1012
MaeI CTAG 1 cut(s) 1103
MaeIII GTNAC 2 cut(s) 260, 373
MalI GATC 1 cut(s) 518
MboI GATC 1 cut(s) 516
MboII GAAGA 3 cut(s) 193, 1032, 1035
MfeI CAATTG 1 cut(s) 633
MhlI GDGCHC 2 cut(s) 188, 218
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 5 cut(s) 31, 153, 633, 1026, 1129
MluNI TGGCCA 1 cut(s) 300
MlyI GAGTC 2 cut(s) 544, 819
MmeI TCCRAC 2 cut(s) 475, 628
Mox20I TGGCCA 1 cut(s) 300
Mph1103I ATGCAT 2 cut(s) 324, 1117
MroXI GAANNNNTTC 1 cut(s) 562
MscI TGGCCA 1 cut(s) 300
MseI TTAA 5 cut(s) 273, 413, 525, 996, 1128
MslI CAYNNNNRTG 1 cut(s) 663
Msp20I TGGCCA 1 cut(s) 300
MspA1I CMGCKG 2 cut(s) 384, 776
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 2 cut(s) 118, 1014
MunI CAATTG 1 cut(s) 633
MvaI CCWGG 2 cut(s) 118, 1014
MwoI GCNNNNNNNGC 2 cut(s) 1112, 1154
NdeII GATC 1 cut(s) 516
NlaIII CATG 7 cut(s) 200, 225, 322, 326, 838, 1075, 1089
NlaIV GGNNCC 1 cut(s) 886
NmuCI GTSAC 2 cut(s) 260, 373
NsiI ATGCAT 2 cut(s) 324, 1117
PciSI GCTCTTC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 562
PfeI GAWTC 4 cut(s) 16, 62, 352, 558
PflMI CCANNNNNTGG 1 cut(s) 1013
PkrI GCNGC 7 cut(s) 172, 175, 383, 420, 598, 657, 1042
PleI GAGTC 2 cut(s) 543, 819
PpsI GAGTC 2 cut(s) 543, 819
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 116, 1012
PspFI CCCAGC 2 cut(s) 138, 384
PspGI CCWGG 2 cut(s) 116, 1012
PspN4I GGNNCC 1 cut(s) 886
PstNI CAGNNNCTG 1 cut(s) 661
PvuII CAGCTG 2 cut(s) 384, 776
RsaI GTAC 4 cut(s) 310, 404, 868, 899
RsaNI GTAC 4 cut(s) 309, 403, 867, 898
RseI CAYNNNNRTG 1 cut(s) 663
SacI GAGCTC 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 176
SaqAI TTAA 5 cut(s) 273, 413, 525, 996, 1128
SatI GCNGC 7 cut(s) 171, 174, 382, 419, 597, 656, 1041
Sau3AI GATC 1 cut(s) 516
ScaI AGTACT 3 cut(s) 310, 404, 868
SchI GAGTC 2 cut(s) 544, 819
ScrFI CCNGG 2 cut(s) 118, 1014
SduI GDGCHC 2 cut(s) 188, 218
SexAI ACCWGGT 1 cut(s) 1012
SfaNI GCATC 5 cut(s) 125, 316, 667, 766, 1124
SfcI CTRYAG 1 cut(s) 771
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
Sse9I AATT 5 cut(s) 31, 153, 633, 1026, 1129
SspI AATATT 1 cut(s) 166
SspMI CTAG 1 cut(s) 1103
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 116, 1012
TaaI ACNGT 2 cut(s) 313, 472
TaqI TCGA 1 cut(s) 95
TasI AATT 5 cut(s) 31, 153, 633, 1026, 1129
TatI WGTACW 3 cut(s) 308, 402, 866
TfiI GAWTC 4 cut(s) 16, 62, 352, 558
Tru1I TTAA 5 cut(s) 273, 413, 525, 996, 1128
Tru9I TTAA 5 cut(s) 273, 413, 525, 996, 1128
TscAI CASTG 2 cut(s) 435, 477
TseFI GTSAC 2 cut(s) 260, 373
TseI GCWGC 7 cut(s) 170, 173, 381, 418, 596, 655, 1040
Tsp45I GTSAC 2 cut(s) 260, 373
TspDTI ATGAA 3 cut(s) 29, 413, 681
TspRI CASTG 2 cut(s) 435, 477
Van91I CCANNNNNTGG 1 cut(s) 1013
XapI RAATTY 1 cut(s) 1026
XmnI GAANNNNTTC 1 cut(s) 562
XspI CTAG 1 cut(s) 1103
ZrmI AGTACT 3 cut(s) 310, 404, 868
Zsp2I ATGCAT 2 cut(s) 324, 1117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.