Rh2CG261300

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001035
Physical Location & Seq
Reverse (-)
28036826 .. 28041174
4349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG261300.1

Sequence Viewer

Length: 1158 bp
ATGTCGAGTTTCATGCCGGCCGCCAATCAGAACACGAATCCCAACAAGTCCTTCGAGGTCGCCGAACCTCCAAACGACTCCATTTCGAGCCTCAGCTTCAGCCCCAAAGCCAATCACCTCGTCGCCACCTCCTGGGACAACCAGGTTCGATGCTGGGAGATACAACACAGTGGGCAAAATCTTGCTAGCACCCCCAAGGGAATGATTTCACACGACCATCCGGCTTTGTGCTCGACTTGGAAGGATGATGGGGCGACTGTCTTCTCTGGAGGATGTGACAAGCAAGCTAAGATGTGGTCCTTGGCTACTAACCAAACAATGACTGTTGCTGTGCATGATGCCCCCATTAAAGACATGGCTTGGATCCCTGAGTTGAACCTCCTAGTCACCGGAAGCTTGGACAAGACTGTAAAGTATTGGGATACGAGGCAGCAGAGCCCAGCGCACACCCAACAGCTCCCTGAACGCTGCTATGCACTATCAGTGAAACAGTCTCTAATGGTTGTTGGGACGGCGGATAGAAATATTATTATTTTTCACTTGCAGCAACCCCAGAATGAGTTGAAGAGAATTGTTTCACCCCTAAAGTTTCAGACAAGGTGTGTTGCTGCATTCCCTGATCAGCAAGGATTCTTGGTTGGATCTATTGAGGGGAGGGTTGGTGTGCATCATCTGGATGAGCAACTATTATCTAAAAACTTTACCTTCAAATGCCACAGGGAGGGCAATGAGATATACTCTGTCAACTCTCTGAACTTCCATCCTGTCCACCACACGTTTGCTACCGCTGGCTCTGATGGTTCTTTTAATTTTTGGGACAAGGACAGCAAACAAAGACTCAAGGCAATGCAAAGGTGCAGTCAGCCTATTCCTTGCAGTACCTTTAACCACGATGGTTCCATATATGCATATGCGGTAATCCTCTTTCACAAATCATATAATTGGATGCACAACACAAAGACGAACACTCCAGGGATGTTTCATCTTTTATGTATTACATTAAAACTCTTTCCCCCAATTGGCGTGTGTAGGTTTGCTACGATTGGAGCAAGGGTGCAGAAAATCATAATCCTGCGGCAGCAAAGAACCACATTTTCCTCCATTTGCCACAGGTATTCTAATGAGTTGCTTCAAATAGTTATCTTCATGAAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

385

Amino Acids

43.41

Weight (kDa)

8.99

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 20 - 180 3.3e-19 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 21 - 149 2.7e-11 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 22 - 176 2.2e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 25 - 235 7.1e-21 WDR5 beta-propeller domain
WDR55 PF24796 25 - 227 1.1e-16 WDR55
Beta-prop_WDR3_2nd PF25172 26 - 179 3.7e-09 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 27 - 186 1.4e-06 Aladin seven-bladed propeller
WD40_Gbeta PF25391 37 - 144 1.5e-07 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 73 - 153 5.8e-09 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 78 - 305 2.5e-30 THOC3 beta-propeller domain
WD40_Prp19 PF24814 81 - 151 2.4e-09 Prp19 WD40 domain
WD40 PF00400 106 - 141 5.3e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 120 - 235 6.8e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 152 - 286 1.4e-09 WDHD1 first WD40 domain
WD40_Prp19 PF24814 159 - 304 3.5e-13 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 172 - 300 6.2e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 132
AciI CCGC 5 cut(s) 21, 515, 786, 914, 1075
AclWI GGATC 3 cut(s) 358, 371, 649
AcoI YGGCCR 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 82
AfaI GTAC 1 cut(s) 880
AfiI CCNNNNNNNGG 3 cut(s) 132, 721, 1019
AflIII ACRYGT 1 cut(s) 774
AgsI TTSAA 4 cut(s) 376, 565, 709, 1133
AjnI CCWGG 3 cut(s) 131, 141, 970
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
AluBI AGCT 4 cut(s) 96, 287, 396, 457
AluI AGCT 4 cut(s) 96, 287, 396, 457
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 1 cut(s) 498
AlwI GGATC 3 cut(s) 358, 371, 649
AoxI GGCC 2 cut(s) 18, 1152
ApeKI GCWGC 5 cut(s) 430, 468, 544, 608, 1078
ArsI GACNNNNNNTTYG 2 cut(s) 844, 876
Asp700I GAANNNNTTC 2 cut(s) 205, 574
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 1 cut(s) 297
AsuHPI GGTGA 3 cut(s) 107, 379, 570
AsuNHI GCTAGC 1 cut(s) 185
AvaII GGWCC 1 cut(s) 297
BamHI GGATCC 1 cut(s) 363
BanII GRGCYC 1 cut(s) 440
BbsI GAAGAC 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 233
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 5 cut(s) 442, 455, 556, 595, 1090
BccI CCATC 5 cut(s) 225, 242, 768, 791, 887
BceAI ACGGC 1 cut(s) 528
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 3 cut(s) 133, 143, 972
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 1 cut(s) 498
BfaI CTAG 2 cut(s) 186, 383
BfuI GTATCC 1 cut(s) 415
BisI GCNGC 7 cut(s) 21, 431, 469, 545, 609, 1076, 1079
BlsI GCNGC 7 cut(s) 22, 432, 470, 546, 610, 1077, 1080
Bme1390I CCNGG 3 cut(s) 133, 143, 972
Bme18I GGWCC 1 cut(s) 297
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 2 cut(s) 365, 898
BmrFI CCNGG 3 cut(s) 133, 143, 972
BmsI GCATC 4 cut(s) 140, 328, 676, 936
BmtI GCTAGC 1 cut(s) 189
BpiI GAAGAC 1 cut(s) 253
BplI GAGNNNNNCTC 2 cut(s) 722, 754
BpmI CTGGAG 2 cut(s) 288, 954
Bpu10I CCTNAGC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 824
BsaJI CCNNGG 4 cut(s) 132, 195, 300, 971
BsaWI WCCGGW 1 cut(s) 389
BsaXI ACNNNNNCTCC 6 cut(s) 646, 676, 952, 982, 1038, 1068
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 721, 1019
Bse118I RCCGGY 1 cut(s) 16
Bse3DI GCAATG 2 cut(s) 733, 852
BseBI CCWGG 3 cut(s) 133, 143, 972
BseDI CCNNGG 4 cut(s) 132, 195, 300, 971
BseGI GGATG 7 cut(s) 217, 250, 278, 682, 760, 951, 981
BseLI CCNNNNNNNGG 3 cut(s) 132, 721, 1019
BseMI GCAATG 2 cut(s) 733, 852
BseMII CTCAG 2 cut(s) 106, 360
BseX3I CGGCCG 1 cut(s) 18
BseXI GCAGC 5 cut(s) 442, 455, 556, 595, 1090
BseYI CCCAGC 2 cut(s) 153, 439
BsgI GTGCAG 2 cut(s) 877, 1076
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 2 cut(s) 20, 1154
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 233
BsiSI CCGG 3 cut(s) 17, 221, 390
BslFI GGGAC 3 cut(s) 149, 523, 830
BslI CCNNNNNNNGG 3 cut(s) 132, 721, 1019
BsmAI GTCTC 1 cut(s) 498
BsmFI GGGAC 3 cut(s) 149, 523, 830
BsmI GAATGC 1 cut(s) 611
BsnI GGCC 2 cut(s) 20, 1154
Bsp1286I GDGCHC 2 cut(s) 233, 440
Bsp143I GATC 3 cut(s) 363, 619, 641
BspACI CCGC 5 cut(s) 21, 515, 786, 914, 1075
BspANI GGCC 2 cut(s) 20, 1154
BspCNI CTCAG 2 cut(s) 105, 361
BspHI TCATGA 1 cut(s) 1146
BspLI GGNNCC 2 cut(s) 365, 898
BspOI GCTAGC 1 cut(s) 189
BspPI GGATC 3 cut(s) 358, 371, 649
BsrDI GCAATG 2 cut(s) 733, 852
BsrFI RCCGGY 1 cut(s) 16
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 4 cut(s) 132, 195, 300, 971
BssMI GATC 3 cut(s) 363, 619, 641
BssT1I CCWWGG 2 cut(s) 195, 300
Bst2UI CCWGG 3 cut(s) 133, 143, 972
Bst4CI ACNGT 5 cut(s) 170, 259, 325, 409, 492
Bst6I CTCTTC 1 cut(s) 560
BstC8I GCNNGC 4 cut(s) 18, 187, 285, 790
BstDEI CTNAG 3 cut(s) 92, 288, 369
BstF5I GGATG 7 cut(s) 217, 250, 278, 682, 760, 951, 981
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 3 cut(s) 366, 622, 644
BstMAI GTCTC 1 cut(s) 498
BstMBI GATC 3 cut(s) 363, 619, 641
BstMCI CGRYCG 1 cut(s) 21
BstNI CCWGG 3 cut(s) 133, 143, 972
BstSCI CCNGG 3 cut(s) 131, 141, 970
BstV1I GCAGC 5 cut(s) 442, 455, 556, 595, 1090
BstV2I GAAGAC 1 cut(s) 253
BstX2I RGATCY 2 cut(s) 363, 641
BstYI RGATCY 2 cut(s) 363, 641
BstZI CGGCCG 1 cut(s) 18
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 2 cut(s) 20, 1154
BtsCI GGATG 7 cut(s) 217, 250, 278, 682, 760, 951, 981
BtsIMutI CAGTG 2 cut(s) 175, 489
Cac8I GCNNGC 4 cut(s) 18, 187, 285, 790
CciI TCATGA 1 cut(s) 1146
CfoI GCGC 1 cut(s) 445
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 297
CsiI ACCWGGT 1 cut(s) 141
Csp6I GTAC 1 cut(s) 879
CviAII CATG 4 cut(s) 13, 335, 355, 1147
CviQI GTAC 1 cut(s) 879
DdeI CTNAG 3 cut(s) 92, 288, 369
DpnI GATC 3 cut(s) 365, 621, 643
DpnII GATC 3 cut(s) 363, 619, 641
EaeI YGGCCR 1 cut(s) 18
EagI CGGCCG 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 560
EarI CTCTTC 1 cut(s) 560
EciI GGCGGA 1 cut(s) 530
EclXI CGGCCG 1 cut(s) 18
Eco130I CCWWGG 2 cut(s) 195, 300
Eco147I AGGCCT 1 cut(s) 1154
Eco24I GRGCYC 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 297
Eco52I CGGCCG 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 82
EcoRII CCWGG 3 cut(s) 131, 141, 970
EcoT14I CCWWGG 2 cut(s) 195, 300
EcoT22I ATGCAT 1 cut(s) 910
EcoT38I GRGCYC 1 cut(s) 440
ErhI CCWWGG 2 cut(s) 195, 300
FaeI CATG 4 cut(s) 16, 338, 358, 1150
FaqI GGGAC 3 cut(s) 149, 523, 830
FatI CATG 4 cut(s) 12, 334, 354, 1146
FauNDI CATATG 1 cut(s) 910
FbaI TGATCA 1 cut(s) 619
Fnu4HI GCNGC 7 cut(s) 21, 431, 469, 545, 609, 1076, 1079
FokI GGATG 7 cut(s) 204, 257, 285, 689, 747, 958, 988
FriOI GRGCYC 1 cut(s) 440
Fsp4HI GCNGC 7 cut(s) 21, 431, 469, 545, 609, 1076, 1079
FspBI CTAG 2 cut(s) 186, 383
GlaI GCGC 1 cut(s) 444
GluI GCNGC 7 cut(s) 21, 431, 469, 545, 609, 1076, 1079
GsaI CCCAGC 2 cut(s) 157, 443
GsuI CTGGAG 2 cut(s) 288, 954
HaeIII GGCC 2 cut(s) 20, 1154
HapII CCGG 3 cut(s) 17, 221, 390
HhaI GCGC 1 cut(s) 445
Hin1II CATG 4 cut(s) 16, 338, 358, 1150
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 4 cut(s) 37, 77, 630, 837
HpaII CCGG 3 cut(s) 17, 221, 390
HphI GGTGA 3 cut(s) 107, 379, 570
Hpy166II GTNNAC 2 cut(s) 745, 769
Hpy188I TCNGA 4 cut(s) 30, 594, 753, 796
Hpy188III TCNNGA 3 cut(s) 267, 674, 1147
Hpy8I GTNNAC 2 cut(s) 745, 769
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 4 cut(s) 61, 235, 715, 1144
HpyCH4III ACNGT 5 cut(s) 170, 259, 325, 409, 492
HpyCH4IV ACGT 1 cut(s) 776
HpyF3I CTNAG 3 cut(s) 92, 288, 369
HpySE526I ACGT 1 cut(s) 776
Hsp92II CATG 4 cut(s) 16, 338, 358, 1150
HspAI GCGC 1 cut(s) 443
KroI GCCGGC 1 cut(s) 16
KroNI GCCGGC 1 cut(s) 18
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 3 cut(s) 363, 619, 641
LmnI GCTCC 2 cut(s) 462, 1046
Lsp1109I GCAGC 5 cut(s) 442, 455, 556, 595, 1090
LweI GCATC 4 cut(s) 140, 328, 676, 936
MabI ACCWGGT 1 cut(s) 141
MaeI CTAG 2 cut(s) 186, 383
MaeII ACGT 1 cut(s) 776
MaeIII GTNAC 2 cut(s) 275, 385
MalI GATC 3 cut(s) 365, 621, 643
MboI GATC 3 cut(s) 363, 619, 641
MboII GAAGA 3 cut(s) 253, 577, 1135
MfeI CAATTG 1 cut(s) 1017
MflI RGATCY 2 cut(s) 363, 641
MhlI GDGCHC 2 cut(s) 233, 440
MluCI AATT 4 cut(s) 570, 808, 940, 1017
MlyI GAGTC 2 cut(s) 71, 831
MmeI TCCRAC 1 cut(s) 619
Mph1103I ATGCAT 1 cut(s) 910
MroNI GCCGGC 1 cut(s) 16
MroXI GAANNNNTTC 2 cut(s) 205, 574
MseI TTAA 4 cut(s) 348, 807, 885, 1001
MslI CAYNNNNRTG 1 cut(s) 675
MspA1I CMGCKG 1 cut(s) 788
MspI CCGG 3 cut(s) 17, 221, 390
MspR9I CCNGG 3 cut(s) 133, 143, 972
MunI CAATTG 1 cut(s) 1017
Mva1269I GAATGC 1 cut(s) 611
MvaI CCWGG 3 cut(s) 133, 143, 972
NaeI GCCGGC 1 cut(s) 18
NdeI CATATG 1 cut(s) 910
NdeII GATC 3 cut(s) 363, 619, 641
NgoMIV GCCGGC 1 cut(s) 16
NheI GCTAGC 1 cut(s) 185
NlaIII CATG 4 cut(s) 16, 338, 358, 1150
NlaIV GGNNCC 2 cut(s) 365, 898
NmuCI GTSAC 2 cut(s) 275, 385
NsiI ATGCAT 1 cut(s) 910
PagI TCATGA 1 cut(s) 1146
PceI AGGCCT 1 cut(s) 1154
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 1 cut(s) 611
PdiI GCCGGC 1 cut(s) 18
PdmI GAANNNNTTC 2 cut(s) 205, 574
PfeI GAWTC 2 cut(s) 37, 630
PflMI CCANNNNNTGG 1 cut(s) 132
PkrI GCNGC 7 cut(s) 22, 432, 470, 546, 610, 1077, 1080
PleI GAGTC 2 cut(s) 71, 831
PpsI GAGTC 2 cut(s) 71, 831
Psp6I CCWGG 3 cut(s) 131, 141, 970
PspFI CCCAGC 2 cut(s) 153, 439
PspGI CCWGG 3 cut(s) 131, 141, 970
PspN4I GGNNCC 2 cut(s) 365, 898
PspPI GGNCC 1 cut(s) 297
PsuI RGATCY 2 cut(s) 363, 641
RsaI GTAC 1 cut(s) 880
RsaNI GTAC 1 cut(s) 879
RseI CAYNNNNRTG 1 cut(s) 675
SaqAI TTAA 4 cut(s) 348, 807, 885, 1001
SatI GCNGC 7 cut(s) 21, 431, 469, 545, 609, 1076, 1079
Sau3AI GATC 3 cut(s) 363, 619, 641
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 2 cut(s) 71, 831
ScrFI CCNGG 3 cut(s) 133, 143, 972
SduI GDGCHC 2 cut(s) 233, 440
SexAI ACCWGGT 1 cut(s) 141
SfaNI GCATC 4 cut(s) 140, 328, 676, 936
SinI GGWCC 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
Sse9I AATT 4 cut(s) 570, 808, 940, 1017
SseBI AGGCCT 1 cut(s) 1154
SsiI CCGC 5 cut(s) 21, 515, 786, 914, 1075
SspI AATATT 1 cut(s) 526
SspMI CTAG 2 cut(s) 186, 383
StuI AGGCCT 1 cut(s) 1154
StyD4I CCNGG 3 cut(s) 131, 141, 970
StyI CCWWGG 2 cut(s) 195, 300
TaaI ACNGT 5 cut(s) 170, 259, 325, 409, 492
TaiI ACGT 1 cut(s) 779
TaqI TCGA 5 cut(s) 5, 54, 86, 148, 233
TasI AATT 4 cut(s) 570, 808, 940, 1017
TauI GCSGC 2 cut(s) 23, 1078
TfiI GAWTC 2 cut(s) 37, 630
Tru1I TTAA 4 cut(s) 348, 807, 885, 1001
Tru9I TTAA 4 cut(s) 348, 807, 885, 1001
TscAI CASTG 2 cut(s) 175, 489
TseFI GTSAC 2 cut(s) 275, 385
TseI GCWGC 5 cut(s) 430, 468, 544, 608, 1078
Tsp45I GTSAC 2 cut(s) 275, 385
TspDTI ATGAA 2 cut(s) 971, 1135
TspRI CASTG 2 cut(s) 175, 489
Van91I CCANNNNNTGG 1 cut(s) 132
VpaK11BI GGWCC 1 cut(s) 297
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XmnI GAANNNNTTC 2 cut(s) 205, 574
XspI CTAG 2 cut(s) 186, 383
Zsp2I ATGCAT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.