Rw7G007980

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001035
Physical Location & Seq
Reverse (-)
7268350 .. 7273112
4763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G007980.1

Sequence Viewer

Length: 1095 bp
ATGTCGGCTTTTATGAATCACAATCCGAACAATTCCTTTGAGGTCGCTCAGCCTCCAAGCGATTCTGTTTCAAGCCTCAGTTTCAGTCCCAAATCGAACCTTCTGGTCTCTACCTCCTGGGACAATCAGGTGCGATGCTGGGATATTCAAAGAATTGGAACAAATATTGCTGCTGCGCCGAAGAGCTCCTTTTCGCATGACCATCCGGTTTTGTGCTCAACATGGAAGGATGATGGGATGGCTGTATTCTCTGGAGGTTGTGACAGGCAAGTTAAGATGTGGTCTCTAATGTCTGGTGGCCAAACGAGTACTGTTGCCATGCATGATGCACCAATAAAAGAGATTGCTTGGATTCCCGAGATGGCGCTCTTAGTCACAGGCAGCTGGGACAAGACTATGAAATCTATATTTTGCCTTAATCTCAAGTTATCTATTTATAAACATATGATCTTGGCGGCCTATATTCAGGTACTGGGATTTAAGCAGCCAAATCCAGTGCATACCCAACAACTACCCGAACGCTGTTATGCACTAACAGTGAAACACCCTCTAATGGTTGTTGGAACAGCAGATAGAAATCTGATCATATTTAACTTGCAGAGTCCACAGACTGAGTTCAAGAGAATCATTTCACCCCTGAAGTATCAGACAAGGTGTGTTGCTGCATTTCCTGATAAGCAAGGTTTCTTGGTTGGTTCAATTGAAGGGAGGGTTGGAGTGCAGCATCTGGATGAAGGACAAGATAGTAAAAACTTTACCTTCAAATGCCACAGAGAAAACAATGCTCTTTACTCAGTCAACTCTATAAACTTCCATCCTGTGCATCACACATTTTCTACAGCTGGTTCTGATGGTTCTATCAATTTTTGGGATAAGGACAGCAAACAGAGACTCAAGGCCATGCAAAGGTGCAATCAACCCATCCCTTGCAGTACTTTCAACAATGATGGTTCCATATATGCGTACTCGGTTTGCTATGACTGGCATAAGGGAGCAGAAAATCATAATCCTTCTACTGCAAAGACCTCCATTTTCCTCCACTTGCCGCAGGACGCTGATGTTAAAAGCAAGCCAAGACCTGGTGGAAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

364

Amino Acids

40.74

Weight (kDa)

8.93

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 10 - 128 1e-10 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 20 - 134 7.6e-14 THOC3 beta-propeller domain
WD40 PF00400 20 - 48 2.6e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 21 - 134 1.3e-16 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 22 - 121 1.9e-07 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 25 - 134 4.5e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 35 - 149 1.7e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 64 - 135 5.7e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 65 - 134 5.4e-09 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 67 - 134 4.6e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 74 - 134 1.7e-06 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 168 - 306 5.5e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 177 - 322 6.7e-12 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 183 - 323 2e-11 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 187 - 319 1.5e-06 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 190 - 318 6.3e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 438
AccB7I CCANNNNNTGG 1 cut(s) 1079
AciI CCGC 2 cut(s) 455, 1046
AcoI YGGCCR 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 659
AfaI GTAC 4 cut(s) 310, 471, 934, 965
AfiI CCNNNNNNNGG 3 cut(s) 553, 906, 1079
AgsI TTSAA 7 cut(s) 72, 149, 619, 699, 704, 763, 940
AjnI CCWGG 2 cut(s) 116, 1078
AjuI GAANNNNNNNTTGG 2 cut(s) 696, 728
AluBI AGCT 3 cut(s) 186, 384, 842
AluI AGCT 3 cut(s) 186, 384, 842
Alw21I GWGCWC 2 cut(s) 188, 218
Alw26I GTCTC 3 cut(s) 112, 288, 883
AlwNI CAGNNNCTG 2 cut(s) 472, 727
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 3 cut(s) 298, 456, 897
ApeKI GCWGC 6 cut(s) 170, 173, 381, 484, 662, 721
Asp700I GAANNNNTTC 1 cut(s) 628
AspLEI GCGC 2 cut(s) 178, 367
AsuHPI GGTGA 1 cut(s) 624
AvaI CYCGRG 1 cut(s) 356
BalI TGGCCA 1 cut(s) 300
BanII GRGCYC 1 cut(s) 188
Bbv12I GWGCWC 2 cut(s) 188, 218
BbvI GCAGC 6 cut(s) 157, 160, 393, 496, 649, 733
BccI CCATC 8 cut(s) 210, 227, 232, 355, 822, 845, 929, 941
BciT130I CCWGG 2 cut(s) 118, 1080
BclI TGATCA 1 cut(s) 582
BcoDI GTCTC 3 cut(s) 112, 288, 883
BfmI CTRYAG 1 cut(s) 837
BfoI RGCGCY 1 cut(s) 368
BisI GCNGC 8 cut(s) 171, 174, 382, 456, 485, 663, 722, 1046
BlpI GCTNAGC 1 cut(s) 48
BlsI GCNGC 8 cut(s) 172, 175, 383, 457, 486, 664, 723, 1047
BmcAI AGTACT 2 cut(s) 310, 934
Bme1390I CCNGG 2 cut(s) 118, 1080
BmeT110I CYCGRG 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 952
BmrFI CCNGG 2 cut(s) 118, 1080
BmrI ACTGGG 1 cut(s) 482
BmsI GCATC 4 cut(s) 125, 316, 733, 832
BmuI ACTGGG 1 cut(s) 482
BpmI CTGGAG 1 cut(s) 273
Bpu1102I GCTNAGC 1 cut(s) 48
BpuEI CTTGAG 2 cut(s) 407, 878
BsaBI GATNNNNATC 1 cut(s) 576
BsaI GGTCTC 2 cut(s) 112, 288
BsaJI CCNNGG 1 cut(s) 117
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 3 cut(s) 553, 906, 1079
Bse1I ACTGG 3 cut(s) 477, 494, 986
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 2 cut(s) 118, 1080
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 6 cut(s) 202, 235, 243, 736, 814, 921
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 3 cut(s) 553, 906, 1079
BseMII CTCAG 4 cut(s) 62, 91, 603, 807
BseNI ACTGG 3 cut(s) 477, 494, 986
BseXI GCAGC 6 cut(s) 157, 160, 393, 496, 649, 733
BseYI CCCAGC 2 cut(s) 138, 384
BsgI GTGCAG 1 cut(s) 740
BshFI GGCC 3 cut(s) 300, 458, 899
BsiHKAI GWGCWC 2 cut(s) 188, 218
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 3 cut(s) 72, 134, 401
BslI CCNNNNNNNGG 3 cut(s) 553, 906, 1079
BsmAI GTCTC 3 cut(s) 112, 288, 883
BsmFI GGGAC 3 cut(s) 72, 134, 401
BsnI GGCC 3 cut(s) 300, 458, 899
Bso31I GGTCTC 2 cut(s) 112, 288
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 2 cut(s) 188, 218
Bsp143I GATC 2 cut(s) 447, 582
Bsp1720I GCTNAGC 1 cut(s) 48
BspACI CCGC 2 cut(s) 455, 1046
BspANI GGCC 3 cut(s) 300, 458, 899
BspCNI CTCAG 4 cut(s) 61, 90, 604, 806
BspLI GGNNCC 1 cut(s) 952
BspQI GCTCTTC 1 cut(s) 176
BspTNI GGTCTC 2 cut(s) 112, 288
BsrI ACTGG 3 cut(s) 477, 494, 986
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 2 cut(s) 447, 582
Bst2UI CCWGG 2 cut(s) 118, 1080
Bst4CI ACNGT 2 cut(s) 313, 538
Bst6I CTCTTC 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 1070
BstDEI CTNAG 5 cut(s) 48, 77, 370, 612, 793
BstF5I GGATG 6 cut(s) 202, 235, 243, 736, 814, 921
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 2 cut(s) 178, 367
BstKTI GATC 2 cut(s) 450, 585
BstMAI GTCTC 3 cut(s) 112, 288, 883
BstMBI GATC 2 cut(s) 447, 582
BstNI CCWGG 2 cut(s) 118, 1080
BstSCI CCNGG 2 cut(s) 116, 1078
BstSFI CTRYAG 1 cut(s) 837
BstV1I GCAGC 6 cut(s) 157, 160, 393, 496, 649, 733
BsuRI GGCC 3 cut(s) 300, 458, 899
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 6 cut(s) 202, 235, 243, 736, 814, 921
BtsIMutI CAGTG 2 cut(s) 501, 543
Cac8I GCNNGC 1 cut(s) 1070
CaiI CAGNNNCTG 2 cut(s) 472, 727
CfoI GCGC 2 cut(s) 178, 367
CseI GACGC 1 cut(s) 1061
CsiI ACCWGGT 1 cut(s) 1078
Csp6I GTAC 4 cut(s) 309, 470, 933, 964
CviAII CATG 5 cut(s) 197, 222, 319, 323, 901
CviQI GTAC 4 cut(s) 309, 470, 933, 964
DdeI CTNAG 5 cut(s) 48, 77, 370, 612, 793
DpnI GATC 2 cut(s) 449, 584
DpnII GATC 2 cut(s) 447, 582
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 2 cut(s) 112, 288
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 659
Eco88I CYCGRG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 116, 1078
EcoT22I ATGCAT 1 cut(s) 324
EcoT38I GRGCYC 1 cut(s) 188
FaeI CATG 5 cut(s) 200, 225, 322, 326, 904
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 3 cut(s) 72, 134, 401
FatI CATG 5 cut(s) 196, 221, 318, 322, 900
FauNDI CATATG 1 cut(s) 444
FbaI TGATCA 1 cut(s) 582
Fnu4HI GCNGC 8 cut(s) 171, 174, 382, 456, 485, 663, 722, 1046
FokI GGATG 6 cut(s) 189, 242, 250, 743, 801, 908
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 8 cut(s) 171, 174, 382, 456, 485, 663, 722, 1046
GlaI GCGC 2 cut(s) 177, 366
GluI GCNGC 8 cut(s) 171, 174, 382, 456, 485, 663, 722, 1046
GsaI CCCAGC 2 cut(s) 142, 388
GsuI CTGGAG 1 cut(s) 273
HaeII RGCGCY 1 cut(s) 368
HaeIII GGCC 3 cut(s) 300, 458, 899
HapII CCGG 1 cut(s) 206
HgaI GACGC 1 cut(s) 1061
HhaI GCGC 2 cut(s) 178, 367
Hin1II CATG 5 cut(s) 200, 225, 322, 326, 904
Hin6I GCGC 2 cut(s) 176, 365
HinP1I GCGC 2 cut(s) 176, 365
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HinfI GANTC 6 cut(s) 16, 62, 352, 601, 624, 891
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 624
Hpy166II GTNNAC 2 cut(s) 605, 799
Hpy188I TCNGA 4 cut(s) 27, 582, 648, 850
Hpy188III TCNNGA 5 cut(s) 252, 356, 619, 671, 728
Hpy8I GTNNAC 2 cut(s) 605, 799
HpyAV CCTTC 7 cut(s) 110, 220, 698, 728, 769, 1020, 1083
HpyCH4III ACNGT 2 cut(s) 313, 538
HpyF3I CTNAG 5 cut(s) 48, 77, 370, 612, 793
Hsp92II CATG 5 cut(s) 200, 225, 322, 326, 904
HspAI GCGC 2 cut(s) 176, 365
Ksp22I TGATCA 1 cut(s) 582
Kzo9I GATC 2 cut(s) 447, 582
LguI GCTCTTC 1 cut(s) 176
LmnI GCTCC 2 cut(s) 191, 992
Lsp1109I GCAGC 6 cut(s) 157, 160, 393, 496, 649, 733
LweI GCATC 4 cut(s) 125, 316, 733, 832
MabI ACCWGGT 1 cut(s) 1078
MaeIII GTNAC 2 cut(s) 260, 373
MalI GATC 2 cut(s) 449, 584
MboI GATC 2 cut(s) 447, 582
MboII GAAGA 1 cut(s) 193
MfeI CAATTG 1 cut(s) 699
MhlI GDGCHC 2 cut(s) 188, 218
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 4 cut(s) 31, 153, 699, 862
MluNI TGGCCA 1 cut(s) 300
MlyI GAGTC 2 cut(s) 610, 885
MmeI TCCRAC 2 cut(s) 541, 694
MnlI CCTC 9 cut(s) 34, 63, 86, 124, 248, 558, 702, 1036, 1046
Mox20I TGGCCA 1 cut(s) 300
Mph1103I ATGCAT 1 cut(s) 324
MroXI GAANNNNTTC 1 cut(s) 628
MscI TGGCCA 1 cut(s) 300
MseI TTAA 5 cut(s) 273, 417, 480, 591, 1062
MslI CAYNNNNRTG 1 cut(s) 729
Msp20I TGGCCA 1 cut(s) 300
MspA1I CMGCKG 2 cut(s) 384, 842
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 2 cut(s) 118, 1080
MunI CAATTG 1 cut(s) 699
MvaI CCWGG 2 cut(s) 118, 1080
NdeI CATATG 1 cut(s) 444
NdeII GATC 2 cut(s) 447, 582
NlaIII CATG 5 cut(s) 200, 225, 322, 326, 904
NlaIV GGNNCC 1 cut(s) 952
NmuCI GTSAC 2 cut(s) 260, 373
NsiI ATGCAT 1 cut(s) 324
PciSI GCTCTTC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 628
PfeI GAWTC 4 cut(s) 16, 62, 352, 624
PflMI CCANNNNNTGG 1 cut(s) 1079
PkrI GCNGC 8 cut(s) 172, 175, 383, 457, 486, 664, 723, 1047
PleI GAGTC 2 cut(s) 609, 885
PpsI GAGTC 2 cut(s) 609, 885
PsiI TTATAA 1 cut(s) 438
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 116, 1078
PspFI CCCAGC 2 cut(s) 138, 384
PspGI CCWGG 2 cut(s) 116, 1078
PspN4I GGNNCC 1 cut(s) 952
PstNI CAGNNNCTG 2 cut(s) 472, 727
PvuII CAGCTG 2 cut(s) 384, 842
RsaI GTAC 4 cut(s) 310, 471, 934, 965
RsaNI GTAC 4 cut(s) 309, 470, 933, 964
RseI CAYNNNNRTG 1 cut(s) 729
SacI GAGCTC 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 176
SaqAI TTAA 5 cut(s) 273, 417, 480, 591, 1062
SatI GCNGC 8 cut(s) 171, 174, 382, 456, 485, 663, 722, 1046
Sau3AI GATC 2 cut(s) 447, 582
ScaI AGTACT 2 cut(s) 310, 934
SchI GAGTC 2 cut(s) 610, 885
ScrFI CCNGG 2 cut(s) 118, 1080
SduI GDGCHC 2 cut(s) 188, 218
SexAI ACCWGGT 1 cut(s) 1078
SfaNI GCATC 4 cut(s) 125, 316, 733, 832
SfcI CTRYAG 1 cut(s) 837
SmiMI CAYNNNNRTG 1 cut(s) 729
SmlI CTYRAG 2 cut(s) 422, 893
SmoI CTYRAG 2 cut(s) 422, 893
Sse9I AATT 4 cut(s) 31, 153, 699, 862
SsiI CCGC 2 cut(s) 455, 1046
SspI AATATT 1 cut(s) 166
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 116, 1078
TaaI ACNGT 2 cut(s) 313, 538
TaqI TCGA 1 cut(s) 95
TasI AATT 4 cut(s) 31, 153, 699, 862
TatI WGTACW 2 cut(s) 308, 932
TauI GCSGC 2 cut(s) 458, 1048
TfiI GAWTC 4 cut(s) 16, 62, 352, 624
Tru1I TTAA 5 cut(s) 273, 417, 480, 591, 1062
Tru9I TTAA 5 cut(s) 273, 417, 480, 591, 1062
TscAI CASTG 2 cut(s) 501, 543
TseFI GTSAC 2 cut(s) 260, 373
TseI GCWGC 6 cut(s) 170, 173, 381, 484, 662, 721
Tsp45I GTSAC 2 cut(s) 260, 373
TspDTI ATGAA 3 cut(s) 29, 413, 747
TspRI CASTG 2 cut(s) 501, 543
Van91I CCANNNNNTGG 1 cut(s) 1079
XmnI GAANNNNTTC 1 cut(s) 628
ZrmI AGTACT 2 cut(s) 310, 934
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.