Rorug07G0234100

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001035
Physical Location & Seq
Reverse (-)
21462936 .. 21464687
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0234100.1

Sequence Viewer

Length: 498 bp
ATGGCAATGAAAATGTGGGTTTCTTCTACAGCAAATGCACTCAGAATCTCTGGTGCCTCCAAAGCTCAACTCTCCCCTGCATTTTCTCTCTCCAGATGCTTTTCCACCGTTCTGGATGGGTTGAAGTATGCATCTTCACATGAATGGGTGAAGCATGAAGGCTCAGTTGCTACAATTGGTATCACTGACCATGCTCAGGACCATCTTGGTGAAGTGGTGTTTGTGGAGCTGCCAGAACCCGGTACAGCAGTCTCCCAAAGCAGCAGCTTCGGAAATGTTGAAAGTGTGAAAGCAACCAGTGATGTCAATTCCCCAATCTCTGGTGAGGTTGTTGAGGTTAACCCGAAGCTGAGTGAAACACCTGGTTTGATCAATACAAGCCCATATGAAGATGGATGGATGATCAAGGTGAAGCCCAGCAATGCATCAGAACTAGAATCCTTGTTGGGTCCTAAAGAATACACGAAATTCTGCGAGGAAGAAGATGCAGCCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

165

Amino Acids

17.66

Weight (kDa)

4.86

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 41 - 160 1.6e-49 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 53
AccB7I CCANNNNNTGG 1 cut(s) 320
AcsI RAATTY 1 cut(s) 467
AfaI GTAC 1 cut(s) 244
AfiI CCNNNNNNNGG 3 cut(s) 196, 239, 320
AgsI TTSAA 2 cut(s) 124, 281
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 4 cut(s) 65, 229, 267, 349
AluI AGCT 4 cut(s) 65, 229, 267, 349
Alw26I GTCTC 1 cut(s) 256
ApeKI GCWGC 4 cut(s) 229, 261, 264, 488
ApoI RAATTY 1 cut(s) 467
AspS9I GGNCC 2 cut(s) 199, 449
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 4 cut(s) 160, 221, 335, 421
AvaII GGWCC 2 cut(s) 199, 449
BaeI ACNNNNGTAYC 2 cut(s) 234, 267
BanI GGYRCC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 216, 273, 276
BccI CCATC 4 cut(s) 110, 210, 386, 390
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 1 cut(s) 363
BclI TGATCA 2 cut(s) 369, 402
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 256
BfaI CTAG 2 cut(s) 434, 496
BfmI CTRYAG 1 cut(s) 27
BisI GCNGC 4 cut(s) 230, 262, 265, 489
BlsI GCNGC 4 cut(s) 231, 263, 266, 490
Bme1390I CCNGG 2 cut(s) 240, 363
Bme18I GGWCC 2 cut(s) 199, 449
BmgT120I GGNCC 2 cut(s) 199, 449
BmiI GGNNCC 2 cut(s) 55, 450
BmrFI CCNGG 2 cut(s) 240, 363
BmsI GCATC 4 cut(s) 86, 140, 434, 475
BpmI CTGGAG 1 cut(s) 76
Bpu10I CCTNAGC 1 cut(s) 195
BpuMI CCSGG 1 cut(s) 240
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 239, 320
Bse1I ACTGG 1 cut(s) 297
Bse3DI GCAATG 2 cut(s) 12, 427
BseBI CCWGG 1 cut(s) 363
BseGI GGATG 3 cut(s) 121, 401, 405
BseLI CCNNNNNNNGG 3 cut(s) 196, 239, 320
BseMI GCAATG 2 cut(s) 12, 427
BseMII CTCAG 4 cut(s) 55, 177, 209, 341
BseNI ACTGG 1 cut(s) 297
BseXI GCAGC 3 cut(s) 216, 273, 276
BseYI CCCAGC 1 cut(s) 416
BshNI GGYRCC 1 cut(s) 53
BsiSI CCGG 1 cut(s) 240
BslI CCNNNNNNNGG 3 cut(s) 196, 239, 320
BsmAI GTCTC 1 cut(s) 256
Bsp143I GATC 2 cut(s) 369, 402
BspCNI CTCAG 4 cut(s) 54, 176, 208, 342
BspLI GGNNCC 2 cut(s) 55, 450
BspT107I GGYRCC 1 cut(s) 53
BsrDI GCAATG 2 cut(s) 12, 427
BsrI ACTGG 1 cut(s) 297
BssMI GATC 2 cut(s) 369, 402
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 1 cut(s) 109
BstDEI CTNAG 4 cut(s) 41, 163, 195, 350
BstF5I GGATG 3 cut(s) 121, 401, 405
BstKTI GATC 2 cut(s) 372, 405
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 2 cut(s) 369, 402
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 2 cut(s) 238, 361
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 3 cut(s) 216, 273, 276
BstXI CCANNNNNNTGG 1 cut(s) 112
BtsCI GGATG 3 cut(s) 121, 401, 405
BtsIMutI CAGTG 2 cut(s) 183, 304
Cfr13I GGNCC 2 cut(s) 199, 449
CsiI ACCWGGT 1 cut(s) 361
Csp6I GTAC 1 cut(s) 243
CviAII CATG 3 cut(s) 140, 155, 191
CviJI RGCY 8 cut(s) 65, 162, 229, 267, 349, 381, 415, 491
CviKI_1 RGCY 8 cut(s) 65, 162, 229, 267, 349, 381, 415, 491
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 4 cut(s) 41, 163, 195, 350
DpnI GATC 2 cut(s) 371, 404
DpnII GATC 2 cut(s) 369, 402
Eco47I GGWCC 2 cut(s) 199, 449
EcoO109I RGGNCCY 1 cut(s) 449
EcoRII CCWGG 1 cut(s) 361
EcoT22I ATGCAT 2 cut(s) 133, 427
FaeI CATG 3 cut(s) 143, 158, 194
FaiI YATR 6 cut(s) 129, 141, 156, 192, 385, 387
FatI CATG 3 cut(s) 139, 154, 190
FauNDI CATATG 1 cut(s) 385
FbaI TGATCA 2 cut(s) 369, 402
Fnu4HI GCNGC 4 cut(s) 230, 262, 265, 489
FokI GGATG 3 cut(s) 128, 408, 412
Fsp4HI GCNGC 4 cut(s) 230, 262, 265, 489
FspBI CTAG 2 cut(s) 434, 496
GluI GCNGC 4 cut(s) 230, 262, 265, 489
GsaI CCCAGC 1 cut(s) 420
GsuI CTGGAG 1 cut(s) 76
HapII CCGG 1 cut(s) 240
Hin1II CATG 3 cut(s) 143, 158, 194
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 2 cut(s) 45, 437
HpaI GTTAAC 1 cut(s) 340
HpaII CCGG 1 cut(s) 240
HphI GGTGA 4 cut(s) 160, 221, 335, 421
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 3 cut(s) 44, 272, 430
Hpy188III TCNNGA 3 cut(s) 93, 113, 197
Hpy8I GTNNAC 1 cut(s) 340
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4V TGCA 5 cut(s) 38, 80, 131, 425, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 4 cut(s) 41, 163, 195, 350
Hsp92II CATG 3 cut(s) 143, 158, 194
Ksp22I TGATCA 2 cut(s) 369, 402
KspAI GTTAAC 1 cut(s) 340
Kzo9I GATC 2 cut(s) 369, 402
LmnI GCTCC 1 cut(s) 226
Lsp1109I GCAGC 3 cut(s) 216, 273, 276
LweI GCATC 4 cut(s) 86, 140, 434, 475
MabI ACCWGGT 1 cut(s) 361
MaeI CTAG 2 cut(s) 434, 496
MalI GATC 2 cut(s) 371, 404
MboI GATC 2 cut(s) 369, 402
MboII GAAGA 5 cut(s) 15, 126, 401, 491, 494
MfeI CAATTG 1 cut(s) 174
MluCI AATT 3 cut(s) 174, 307, 467
MnlI CCTC 4 cut(s) 67, 319, 328, 469
Mph1103I ATGCAT 2 cut(s) 133, 427
MseI TTAA 1 cut(s) 339
MslI CAYNNNNRTG 2 cut(s) 142, 207
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 2 cut(s) 240, 363
MunI CAATTG 1 cut(s) 174
MvaI CCWGG 1 cut(s) 363
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 1 cut(s) 240
NdeI CATATG 1 cut(s) 385
NdeII GATC 2 cut(s) 369, 402
NlaIII CATG 3 cut(s) 143, 158, 194
NlaIV GGNNCC 2 cut(s) 55, 450
NsiI ATGCAT 2 cut(s) 133, 427
PfeI GAWTC 2 cut(s) 45, 437
PflMI CCANNNNNTGG 1 cut(s) 320
PkrI GCNGC 4 cut(s) 231, 263, 266, 490
PpuMI RGGWCCY 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 361
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 2 cut(s) 55, 450
PspPI GGNCC 2 cut(s) 199, 449
PspPPI RGGWCCY 1 cut(s) 449
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
RseI CAYNNNNRTG 2 cut(s) 142, 207
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 4 cut(s) 230, 262, 265, 489
Sau3AI GATC 2 cut(s) 369, 402
Sau96I GGNCC 2 cut(s) 199, 449
ScrFI CCNGG 2 cut(s) 240, 363
SetI ASST 8 cut(s) 67, 231, 269, 330, 339, 351, 364, 411
SexAI ACCWGGT 1 cut(s) 361
SfaNI GCATC 4 cut(s) 86, 140, 434, 475
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 2 cut(s) 199, 449
SmiMI CAYNNNNRTG 2 cut(s) 142, 207
Sse9I AATT 3 cut(s) 174, 307, 467
SspMI CTAG 2 cut(s) 434, 496
StyD4I CCNGG 2 cut(s) 238, 361
TaaI ACNGT 1 cut(s) 109
TasI AATT 3 cut(s) 174, 307, 467
TfiI GAWTC 2 cut(s) 45, 437
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 2 cut(s) 190, 304
TseI GCWGC 4 cut(s) 229, 261, 264, 488
TspDTI ATGAA 4 cut(s) 23, 156, 171, 402
TspRI CASTG 2 cut(s) 190, 304
Van91I CCANNNNNTGG 1 cut(s) 320
VpaK11BI GGWCC 2 cut(s) 199, 449
XapI RAATTY 1 cut(s) 467
XspI CTAG 2 cut(s) 434, 496
Zsp2I ATGCAT 2 cut(s) 133, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.