Rh7BG368000

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001035
Physical Location & Seq
Reverse (-)
39341739 .. 39349757
8019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG368000.1

Sequence Viewer

Length: 936 bp
ATGTCGACCTTCATGGCCGCTTCCAATCCAAATCCAAACAAATCCTTTGAGGTCGCTCAGCCCCCAACCGACTCCGTTTCGAGTCTCAGTTTCAGCCCTAAGGCTAACTTTCTGGTCGCGACCTCCTGGGACAACCAGGTGCGATGTTGGGAGATACAACAGAGTGGTGGAAATGTTGCCAGTATGCCCAAGGCAGCTATATCGCACGATAATCCGGTTTTGTGCTCAACTTGGAAAGATGATGGGACAACTGTGTTCTCTGGAGGTTGTGATAAGCAAGTTAAGATGTGGCCTCTGATGTCTGGTGGCAACCAAACAATGACTGTTGCGATGCATGATGCGCCCATCAAGGAAATAGCTTGGATCCCTGAGATGAGCCTCTTGGTGACAGGAAGCTGGGACAAGACTTTGAAGTATTGGGATACAAGGCAGCCAAATCCAGTGCATACACAACTGCTCCCAGAGAGGTGCTATGCATTATCTGTGAGACACCCTCTGATGGTGGTTGGAACTGCTGATCGAAATCTTATTGTTTATAACTTGCAGAGTCCACAGACTGAGTTCAAGAGAATCATTTCACCCCTAAAGTATCAGACAAGGTGTGTTGCGGCATTTCCTGATCAGCAAGGATTCTTGGTTGGCTCTATTGAAGGCAGGGTTGGTGTACATCATCTGGATGAGGGACAACAAAGTAAAAACTTTACCTTCAAATGCCACAGAGAGGGCAACGAGATATATTCAGTCAATTCTCTGAACTTCCATCCTGTGTATCACACATTTGCTACTGCTGGTTCTGATGGTTCTTTCAACTTTTGGGATAAGGACAGCAAACAGAGACTAAAGGCCATGGCAAGGTGCAGTCAACCAATACCTTGCAGTACTTTCAACAACGATGGTTCCATATTTGCATACTCGGTAATTTTCTCCACTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

311

Amino Acids

34.63

Weight (kDa)

7.61

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 13 - 149 1.4e-13 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 20 - 171 9.8e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 20 - 113 9.6e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 20 - 181 6.1e-19 WDR3 first beta-propeller domain
WD40 PF00400 23 - 50 5.2e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 23 - 142 1.3e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 23 - 230 6.3e-21 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 24 - 144 4.9e-11 WDR3 second beta-propeller domain
WDR55 PF24796 24 - 229 1.9e-17 WDR55
WD40_Gbeta PF25391 26 - 145 2.7e-09 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 68 - 149 1.4e-08 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 69 - 151 5.6e-10 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 69 - 305 5.4e-30 THOC3 beta-propeller domain
WD40 PF00400 105 - 141 7.4e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 120 - 233 2.6e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 155 - 284 1.1e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 163 - 304 8.5e-13 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 169 - 301 4.5e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 172 - 303 5.5e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 537
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 119
AciI CCGC 2 cut(s) 18, 608
AclWI GGATC 2 cut(s) 358, 371
AcoI YGGCCR 1 cut(s) 15
AfaI GTAC 2 cut(s) 666, 880
AfiI CCNNNNNNNGG 3 cut(s) 499, 721, 852
AgsI TTSAA 6 cut(s) 412, 565, 650, 709, 808, 886
AjnI CCWGG 2 cut(s) 125, 135
AjuI GAANNNNNNNTTGG 2 cut(s) 642, 674
AluBI AGCT 3 cut(s) 197, 359, 396
AluI AGCT 3 cut(s) 197, 359, 396
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 3 cut(s) 89, 481, 829
AlwI GGATC 2 cut(s) 358, 371
AoxI GGCC 3 cut(s) 15, 290, 843
ApeKI GCWGC 2 cut(s) 194, 430
Asp700I GAANNNNTTC 1 cut(s) 574
AspLEI GCGC 1 cut(s) 343
AsuHPI GGTGA 2 cut(s) 397, 570
AxyI CCTNAGG 1 cut(s) 99
BamHI GGATCC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 2 cut(s) 206, 442
BccI CCATC 6 cut(s) 236, 353, 493, 768, 791, 887
BcgI CGANNNNNNTGC 2 cut(s) 183, 217
BciT130I CCWGG 2 cut(s) 127, 137
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 3 cut(s) 89, 481, 829
BfuI GTATCC 1 cut(s) 415
BisI GCNGC 4 cut(s) 18, 195, 431, 609
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 4 cut(s) 19, 196, 432, 610
BmcAI AGTACT 1 cut(s) 880
Bme1390I CCNGG 2 cut(s) 127, 137
BmiI GGNNCC 2 cut(s) 365, 898
BmrFI CCNGG 2 cut(s) 127, 137
BmsI GCATC 2 cut(s) 321, 328
BplI GAGNNNNNCTC 2 cut(s) 478, 510
BpmI CTGGAG 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 57
BsaBI GATNNNNATC 1 cut(s) 522
BsaJI CCNNGG 3 cut(s) 126, 189, 846
BsaWI WCCGGW 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 3 cut(s) 499, 721, 852
Bse1I ACTGG 2 cut(s) 180, 440
Bse21I CCTNAGG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 522
BseBI CCWGG 2 cut(s) 127, 137
BseDI CCNNGG 3 cut(s) 126, 189, 846
BseGI GGATG 2 cut(s) 682, 760
BseJI GATNNNNATC 1 cut(s) 522
BseLI CCNNNNNNNGG 3 cut(s) 499, 721, 852
BseMII CTCAG 4 cut(s) 71, 100, 360, 549
BseNI ACTGG 2 cut(s) 180, 440
BseXI GCAGC 2 cut(s) 206, 442
BseYI CCCAGC 1 cut(s) 396
BsgI GTGCAG 1 cut(s) 877
Bsh1236I CGCG 1 cut(s) 119
BshFI GGCC 3 cut(s) 17, 292, 845
BsiHKAI GWGCWC 1 cut(s) 227
BsiSI CCGG 1 cut(s) 215
BslFI GGGAC 4 cut(s) 143, 259, 413, 696
BslI CCNNNNNNNGG 3 cut(s) 499, 721, 852
BsmAI GTCTC 3 cut(s) 89, 481, 829
BsmFI GGGAC 4 cut(s) 143, 259, 413, 696
BsnI GGCC 3 cut(s) 17, 292, 845
Bsp1286I GDGCHC 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 664
Bsp143I GATC 3 cut(s) 363, 517, 619
Bsp1720I GCTNAGC 1 cut(s) 57
Bsp19I CCATGG 1 cut(s) 846
Bsp68I TCGCGA 1 cut(s) 119
BspACI CCGC 2 cut(s) 18, 608
BspANI GGCC 3 cut(s) 17, 292, 845
BspCNI CTCAG 4 cut(s) 70, 99, 361, 550
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 2 cut(s) 365, 898
BspPI GGATC 2 cut(s) 358, 371
BsrGI TGTACA 1 cut(s) 664
BsrI ACTGG 2 cut(s) 180, 440
BssECI CCNNGG 3 cut(s) 126, 189, 846
BssMI GATC 3 cut(s) 363, 517, 619
BssT1I CCWWGG 2 cut(s) 189, 846
Bst2UI CCWGG 2 cut(s) 127, 137
Bst4CI ACNGT 2 cut(s) 253, 325
BstAUI TGTACA 1 cut(s) 664
BstDEI CTNAG 5 cut(s) 57, 86, 99, 369, 558
BstDSI CCRYGG 1 cut(s) 846
BstF5I GGATG 2 cut(s) 682, 760
BstFNI CGCG 1 cut(s) 119
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 3 cut(s) 366, 520, 622
BstMAI GTCTC 3 cut(s) 89, 481, 829
BstMBI GATC 3 cut(s) 363, 517, 619
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstNI CCWGG 2 cut(s) 127, 137
BstSCI CCNGG 2 cut(s) 125, 135
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 2 cut(s) 206, 442
BstX2I RGATCY 1 cut(s) 363
BstYI RGATCY 1 cut(s) 363
Bsu36I CCTNAGG 1 cut(s) 99
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 3 cut(s) 17, 292, 845
BtgI CCRYGG 1 cut(s) 846
BtgZI GCGATG 2 cut(s) 157, 344
BtsCI GGATG 2 cut(s) 682, 760
BtsIMutI CAGTG 1 cut(s) 447
BtuMI TCGCGA 1 cut(s) 119
CfoI GCGC 1 cut(s) 343
CsiI ACCWGGT 1 cut(s) 135
Csp6I GTAC 2 cut(s) 665, 879
CviAII CATG 3 cut(s) 13, 335, 847
CviQI GTAC 2 cut(s) 665, 879
DdeI CTNAG 5 cut(s) 57, 86, 99, 369, 558
DpnI GATC 3 cut(s) 365, 519, 621
DpnII GATC 3 cut(s) 363, 517, 619
EaeI YGGCCR 1 cut(s) 15
Eco130I CCWWGG 2 cut(s) 189, 846
Eco81I CCTNAGG 1 cut(s) 99
EcoRII CCWGG 2 cut(s) 125, 135
EcoT14I CCWWGG 2 cut(s) 189, 846
EcoT22I ATGCAT 2 cut(s) 336, 478
ErhI CCWWGG 2 cut(s) 189, 846
FaeI CATG 3 cut(s) 16, 338, 850
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FaqI GGGAC 4 cut(s) 143, 259, 413, 696
FatI CATG 3 cut(s) 12, 334, 846
FbaI TGATCA 1 cut(s) 619
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 18, 195, 431, 609
FokI GGATG 2 cut(s) 689, 747
Fsp4HI GCNGC 4 cut(s) 18, 195, 431, 609
GlaI GCGC 1 cut(s) 342
GluI GCNGC 4 cut(s) 18, 195, 431, 609
GsaI CCCAGC 1 cut(s) 400
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 3 cut(s) 17, 292, 845
HapII CCGG 1 cut(s) 215
HhaI GCGC 1 cut(s) 343
Hin1II CATG 3 cut(s) 16, 338, 850
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HincII GTYRAC 2 cut(s) 6, 863
HindII GTYRAC 2 cut(s) 6, 863
HinfI GANTC 5 cut(s) 71, 82, 547, 570, 630
HpaII CCGG 1 cut(s) 215
HphI GGTGA 2 cut(s) 397, 570
Hpy166II GTNNAC 4 cut(s) 6, 551, 665, 863
Hpy188I TCNGA 5 cut(s) 297, 498, 594, 753, 796
Hpy188III TCNNGA 6 cut(s) 118, 261, 565, 617, 674, 933
Hpy8I GTNNAC 4 cut(s) 6, 551, 665, 863
HpyAV CCTTC 3 cut(s) 19, 644, 715
HpyCH4III ACNGT 2 cut(s) 253, 325
HpyCH4V TGCA 7 cut(s) 334, 445, 476, 544, 858, 876, 908
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpyF3I CTNAG 5 cut(s) 57, 86, 99, 369, 558
Hsp92II CATG 3 cut(s) 16, 338, 850
HspAI GCGC 1 cut(s) 341
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 3 cut(s) 363, 517, 619
LmnI GCTCC 1 cut(s) 462
Lsp1109I GCAGC 2 cut(s) 206, 442
LweI GCATC 2 cut(s) 321, 328
MabI ACCWGGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 385
MalI GATC 3 cut(s) 365, 519, 621
MboI GATC 3 cut(s) 363, 517, 619
MflI RGATCY 1 cut(s) 363
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 2 cut(s) 745, 918
MlyI GAGTC 3 cut(s) 65, 91, 556
MmeI TCCRAC 1 cut(s) 487
MnlI CCTC 9 cut(s) 43, 133, 257, 303, 389, 459, 504, 673, 715
Mph1103I ATGCAT 2 cut(s) 336, 478
MroXI GAANNNNTTC 1 cut(s) 574
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 675
MspI CCGG 1 cut(s) 215
MspR9I CCNGG 2 cut(s) 127, 137
MvaI CCWGG 2 cut(s) 127, 137
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 340
NcoI CCATGG 1 cut(s) 846
NdeII GATC 3 cut(s) 363, 517, 619
NlaIII CATG 3 cut(s) 16, 338, 850
NlaIV GGNNCC 2 cut(s) 365, 898
NmuCI GTSAC 1 cut(s) 385
NruI TCGCGA 1 cut(s) 119
NsiI ATGCAT 2 cut(s) 336, 478
PdmI GAANNNNTTC 1 cut(s) 574
PfeI GAWTC 2 cut(s) 570, 630
PkrI GCNGC 4 cut(s) 19, 196, 432, 610
PleI GAGTC 3 cut(s) 65, 90, 555
PpsI GAGTC 3 cut(s) 65, 90, 555
PsiI TTATAA 1 cut(s) 537
Psp6I CCWGG 2 cut(s) 125, 135
PspFI CCCAGC 1 cut(s) 396
PspGI CCWGG 2 cut(s) 125, 135
PspN4I GGNNCC 2 cut(s) 365, 898
PsuI RGATCY 1 cut(s) 363
RruI TCGCGA 1 cut(s) 119
RsaI GTAC 2 cut(s) 666, 880
RsaNI GTAC 2 cut(s) 665, 879
RseI CAYNNNNRTG 1 cut(s) 675
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 4 cut(s) 18, 195, 431, 609
Sau3AI GATC 3 cut(s) 363, 517, 619
ScaI AGTACT 1 cut(s) 880
SchI GAGTC 3 cut(s) 65, 91, 556
ScrFI CCNGG 2 cut(s) 127, 137
SduI GDGCHC 1 cut(s) 227
SexAI ACCWGGT 1 cut(s) 135
SfaNI GCATC 2 cut(s) 321, 328
SmiMI CAYNNNNRTG 1 cut(s) 675
Sse9I AATT 2 cut(s) 745, 918
SsiI CCGC 2 cut(s) 18, 608
StyD4I CCNGG 2 cut(s) 125, 135
StyI CCWWGG 2 cut(s) 189, 846
TaaI ACNGT 2 cut(s) 253, 325
TaqI TCGA 3 cut(s) 5, 80, 520
TasI AATT 2 cut(s) 745, 918
TatI WGTACW 2 cut(s) 664, 878
TauI GCSGC 2 cut(s) 20, 611
TfiI GAWTC 2 cut(s) 570, 630
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 447
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 2 cut(s) 194, 430
Tsp45I GTSAC 1 cut(s) 385
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 1 cut(s) 447
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 574
ZrmI AGTACT 1 cut(s) 880
Zsp2I ATGCAT 2 cut(s) 336, 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.