MD15G1412500.v1.1

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001035
Physical Location & Seq
Forward (+)
51213459 .. 51219697
6239 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1412500.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGGCTGCTTCGAATCAGAATCAGAATCCGAACAAGTCCTTTGAGGTCACCCATCTTCCTAACGACTCGATATCGAGCCTAAGCTTCAGTCCCAAGAGCAACATTCTGGTCGCCACCTCCTGGGACAACCAGGTGCGATGCTGGGAGATACACCAGAGTGGACAAAATCTTGCTAGTGTGCCCAAGGCATCCATATCCCATGACCAACCGGTTTTGTGCTCAGCTTGGAAGGATGATGGATCTACTGTGTTCTCTGGAGGCTGTGACAAGCAAGTTAAGATGTGGCCTCTAGCTGGTAACCAACCGGTGACCGTTGCCATGCACGATGCACCAATAAAAGAGGTTGCTTGGATCCCGGAGATGAACTACTTAGTCACAGGAAGCTTGGACAAGAATTTGAAGTATTGGGATCTGAGGCAGCAAACTCCAATCCATACCCAAGCTCTACCTGAGCGCTGCTACACCCTATCAGTGAGATACCCTCTAATGGTTGTTGGCACTGCAGACCGAAATCTCATTGTTTTTAACTTGCAAAACCCACAGACTGAATTCAAGAGAATTGTTTCACCCCTAAAGTATCAAACAAGGTGTGTTGCTGCATTTCCGGATCAGCAAGGATTCTTGGTTGGCTCTATTGAAGGGAGGGTTGGTGTACATCATCTTGATGATGGACTACAAAGTAAAAACTTTACCTTTAAGTGCCATAGAGAGGGCAATGACATCTACCCAGTCAACTCTCTGAACTTCCATCCCGTACATCAAACATTTGCTACCGCTGGTTCTGACGGTTCTTTCAATTTTTGGGATAAGGATAGCAAACAGAGACTAAAGGCAATGGCGAGGTGCAGTCAACCTATACCTTGCAGTGCTTTCAACAGTGATGGTTCCATATTTGCATACGCAGTTTGCTATGATTGGAGCAAGGGTGCTGAAAATCACAATCCTGCTACTGCAAAGAACTGCATTTACCTCCACGTGCCACAGGAAGCTGAGGTTAAAGGCAAGCCAAGAATCAACACCGGAAAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

344

Amino Acids

38.3

Weight (kDa)

8.46

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 11 - 146 6.5e-14 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 16 - 157 2e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 18 - 48 5.8e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 18 - 106 9.2e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 20 - 176 7.2e-20 WDR3 first beta-propeller domain
Beta-prop_Aladin PF25460 20 - 182 2.9e-07 Aladin seven-bladed propeller
Beta-prop_WDR5 PF25175 22 - 206 4.7e-20 WDR5 beta-propeller domain
WDR55 PF24796 22 - 222 3.1e-17 WDR55
Beta-prop_WDR3_2nd PF25172 22 - 175 6e-12 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 25 - 152 3.9e-13 Prp19 WD40 domain
WD40_Gbeta PF25391 33 - 141 1e-07 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 67 - 301 3.3e-29 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 68 - 149 7.1e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 116 - 228 2.6e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 151 - 281 1.7e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 162 - 301 8.6e-13 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 165 - 296 4.2e-06 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 246 - 301 4.2e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 604
AciI CCGC 1 cut(s) 774
AclWI GGATC 5 cut(s) 247, 346, 359, 417, 615
AcsI RAATTY 2 cut(s) 394, 548
AcuI CTGAAG 1 cut(s) 70
AcvI CACGTG 1 cut(s) 976
AfaI GTAC 2 cut(s) 654, 756
AfeI AGCGCT 1 cut(s) 455
AfiI CCNNNNNNNGG 5 cut(s) 120, 293, 487, 709, 1026
AgeI ACCGGT 2 cut(s) 208, 304
AgsI TTSAA 5 cut(s) 400, 553, 638, 796, 874
AjnI CCWGG 2 cut(s) 119, 129
AjuI GAANNNNNNNTTGG 2 cut(s) 630, 662
AleI CACNNNNGTG 1 cut(s) 156
AluBI AGCT 6 cut(s) 84, 224, 293, 384, 443, 989
AluI AGCT 6 cut(s) 84, 224, 293, 384, 443, 989
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 1 cut(s) 817
AlwI GGATC 5 cut(s) 247, 346, 359, 417, 615
Aor13HI TCCGGA 1 cut(s) 604
Aor51HI AGCGCT 1 cut(s) 455
AoxI GGCC 1 cut(s) 284
ApeKI GCWGC 4 cut(s) 5, 418, 456, 596
ApoI RAATTY 2 cut(s) 394, 548
AsiGI ACCGGT 2 cut(s) 208, 304
Asp700I GAANNNNTTC 1 cut(s) 562
AspLEI GCGC 1 cut(s) 456
AsuC2I CCSGG 1 cut(s) 356
AsuHPI GGTGA 3 cut(s) 40, 319, 558
AsuII TTCGAA 1 cut(s) 11
BaeGI GKGCMC 1 cut(s) 183
BamHI GGATCC 1 cut(s) 351
BbrPI CACGTG 1 cut(s) 976
Bbv12I GWGCWC 1 cut(s) 221
BbvCI CCTCAGC 1 cut(s) 990
BbvI GCAGC 3 cut(s) 430, 443, 583
BccI CCATC 5 cut(s) 60, 230, 662, 756, 875
BciT130I CCWGG 2 cut(s) 121, 131
BcnI CCSGG 1 cut(s) 356
BcoDI GTCTC 1 cut(s) 817
BfaI CTAG 2 cut(s) 174, 290
BfmI CTRYAG 1 cut(s) 501
BfoI RGCGCY 1 cut(s) 457
BisI GCNGC 4 cut(s) 6, 419, 457, 597
BlpI GCTNAGC 1 cut(s) 220
BlsI GCNGC 4 cut(s) 7, 420, 458, 598
Bme1390I CCNGG 3 cut(s) 121, 131, 356
BmiI GGNNCC 2 cut(s) 353, 886
BmrFI CCNGG 3 cut(s) 121, 131, 356
BmrI ACTGGG 1 cut(s) 722
BmsI GCATC 3 cut(s) 128, 197, 316
BmuI ACTGGG 1 cut(s) 722
BplI GAGNNNNNCTC 2 cut(s) 466, 498
BpmI CTGGAG 1 cut(s) 276
Bpu10I CCTNAGC 3 cut(s) 80, 450, 990
Bpu1102I GCTNAGC 1 cut(s) 220
Bpu14I TTCGAA 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 976
BsaJI CCNNGG 2 cut(s) 120, 183
BsaWI WCCGGW 4 cut(s) 208, 304, 604, 1019
BsaXI ACNNNNNCTCC 4 cut(s) 634, 664, 910, 940
Bsc4I CCNNNNNNNGG 5 cut(s) 120, 293, 487, 709, 1026
Bse118I RCCGGY 2 cut(s) 208, 304
Bse1I ACTGG 1 cut(s) 728
Bse3DI GCAATG 2 cut(s) 721, 840
BseAI TCCGGA 1 cut(s) 604
BseBI CCWGG 2 cut(s) 121, 131
BseDI CCNNGG 2 cut(s) 120, 183
BseGI GGATG 3 cut(s) 188, 238, 748
BseLI CCNNNNNNNGG 5 cut(s) 120, 293, 487, 709, 1026
BseMI GCAATG 2 cut(s) 721, 840
BseMII CTCAG 4 cut(s) 234, 404, 441, 981
BseNI ACTGG 1 cut(s) 728
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 3 cut(s) 430, 443, 583
BseYI CCCAGC 1 cut(s) 141
BsgI GTGCAG 1 cut(s) 865
BshFI GGCC 1 cut(s) 286
BshTI ACCGGT 2 cut(s) 208, 304
BsiHKAI GWGCWC 1 cut(s) 221
BsiSI CCGG 5 cut(s) 209, 305, 356, 605, 1020
BslFI GGGAC 2 cut(s) 75, 137
BslI CCNNNNNNNGG 5 cut(s) 120, 293, 487, 709, 1026
BsmAI GTCTC 1 cut(s) 817
BsmFI GGGAC 2 cut(s) 75, 137
BsnI GGCC 1 cut(s) 286
Bsp119I TTCGAA 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 183, 221
Bsp13I TCCGGA 1 cut(s) 604
Bsp1407I TGTACA 1 cut(s) 652
Bsp143I GATC 4 cut(s) 239, 351, 409, 607
Bsp1720I GCTNAGC 1 cut(s) 220
BspACI CCGC 1 cut(s) 774
BspANI GGCC 1 cut(s) 286
BspCNI CTCAG 4 cut(s) 233, 405, 442, 982
BspEI TCCGGA 1 cut(s) 604
BspLI GGNNCC 2 cut(s) 353, 886
BspMAI CTGCAG 1 cut(s) 505
BspPI GGATC 5 cut(s) 247, 346, 359, 417, 615
BspT104I TTCGAA 1 cut(s) 11
BsrDI GCAATG 2 cut(s) 721, 840
BsrFI RCCGGY 2 cut(s) 208, 304
BsrGI TGTACA 1 cut(s) 652
BsrI ACTGG 1 cut(s) 728
BssAI RCCGGY 2 cut(s) 208, 304
BssECI CCNNGG 2 cut(s) 120, 183
BssMI GATC 4 cut(s) 239, 351, 409, 607
BssT1I CCWWGG 1 cut(s) 183
Bst2UI CCWGG 2 cut(s) 121, 131
Bst4CI ACNGT 5 cut(s) 247, 313, 788, 878, 1029
BstAUI TGTACA 1 cut(s) 652
BstBAI YACGTR 1 cut(s) 976
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 1004
BstDEI CTNAG 6 cut(s) 80, 220, 370, 413, 450, 990
BstEII GGTNACC 3 cut(s) 46, 296, 307
BstF5I GGATG 3 cut(s) 188, 238, 748
BstH2I RGCGCY 1 cut(s) 457
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 4 cut(s) 242, 354, 412, 610
BstMAI GTCTC 1 cut(s) 817
BstMBI GATC 4 cut(s) 239, 351, 409, 607
BstNI CCWGG 2 cut(s) 121, 131
BstPI GGTNACC 3 cut(s) 46, 296, 307
BstSCI CCNGG 3 cut(s) 119, 129, 354
BstSFI CTRYAG 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 183
BstV1I GCAGC 3 cut(s) 430, 443, 583
BstX2I RGATCY 3 cut(s) 239, 351, 409
BstYI RGATCY 3 cut(s) 239, 351, 409
BsuRI GGCC 1 cut(s) 286
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 3 cut(s) 188, 238, 748
BtsI GCAGTG 2 cut(s) 498, 871
BtsIMutI CAGTG 4 cut(s) 477, 498, 871, 883
Cac8I GCNNGC 1 cut(s) 1004
CfoI GCGC 1 cut(s) 456
Cfr10I RCCGGY 2 cut(s) 208, 304
CsiI ACCWGGT 1 cut(s) 129
Csp6I GTAC 2 cut(s) 653, 755
CspAI ACCGGT 2 cut(s) 208, 304
CviAII CATG 2 cut(s) 200, 319
CviQI GTAC 2 cut(s) 653, 755
DdeI CTNAG 6 cut(s) 80, 220, 370, 413, 450, 990
DpnI GATC 4 cut(s) 241, 353, 411, 609
DpnII GATC 4 cut(s) 239, 351, 409, 607
Eco130I CCWWGG 1 cut(s) 183
Eco32I GATATC 1 cut(s) 72
Eco47III AGCGCT 1 cut(s) 455
Eco57I CTGAAG 1 cut(s) 70
Eco72I CACGTG 1 cut(s) 976
Eco91I GGTNACC 3 cut(s) 46, 296, 307
EcoO65I GGTNACC 3 cut(s) 46, 296, 307
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 2 cut(s) 119, 129
EcoRV GATATC 1 cut(s) 72
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 2 cut(s) 203, 322
FaiI YATR 9 cut(s) 194, 201, 320, 435, 705, 857, 890, 898, 912
FaqI GGGAC 2 cut(s) 75, 137
FatI CATG 2 cut(s) 199, 318
Fnu4HI GCNGC 4 cut(s) 6, 419, 457, 597
FokI GGATG 3 cut(s) 175, 245, 735
Fsp4HI GCNGC 4 cut(s) 6, 419, 457, 597
FspBI CTAG 2 cut(s) 174, 290
GlaI GCGC 1 cut(s) 455
GluI GCNGC 4 cut(s) 6, 419, 457, 597
GsaI CCCAGC 1 cut(s) 145
GsuI CTGGAG 1 cut(s) 276
HaeII RGCGCY 1 cut(s) 457
HaeIII GGCC 1 cut(s) 286
HapII CCGG 5 cut(s) 209, 305, 356, 605, 1020
HhaI GCGC 1 cut(s) 456
Hin1II CATG 2 cut(s) 203, 322
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HincII GTYRAC 2 cut(s) 733, 851
HindII GTYRAC 2 cut(s) 733, 851
HindIII AAGCTT 2 cut(s) 82, 382
HinfI GANTC 6 cut(s) 13, 19, 25, 65, 618, 1011
HpaII CCGG 5 cut(s) 209, 305, 356, 605, 1020
HphI GGTGA 3 cut(s) 40, 319, 558
Hpy166II GTNNAC 4 cut(s) 161, 653, 733, 851
Hpy188I TCNGA 6 cut(s) 18, 24, 30, 414, 741, 784
Hpy188III TCNNGA 4 cut(s) 255, 553, 605, 662
Hpy8I GTNNAC 4 cut(s) 161, 653, 733, 851
HpyAV CCTTC 2 cut(s) 223, 632
HpyCH4III ACNGT 5 cut(s) 247, 313, 788, 878, 1029
HpyCH4IV ACGT 1 cut(s) 975
HpyF3I CTNAG 6 cut(s) 80, 220, 370, 413, 450, 990
HpySE526I ACGT 1 cut(s) 975
Hsp92II CATG 2 cut(s) 203, 322
HspAI GCGC 1 cut(s) 454
Kpn2I TCCGGA 1 cut(s) 604
Kzo9I GATC 4 cut(s) 239, 351, 409, 607
LmnI GCTCC 1 cut(s) 918
Lsp1109I GCAGC 3 cut(s) 430, 443, 583
LweI GCATC 3 cut(s) 128, 197, 316
MabI ACCWGGT 1 cut(s) 129
MaeI CTAG 2 cut(s) 174, 290
MaeII ACGT 1 cut(s) 975
MaeIII GTNAC 5 cut(s) 46, 263, 296, 307, 373
MalI GATC 4 cut(s) 241, 353, 411, 609
MboI GATC 4 cut(s) 239, 351, 409, 607
MboII GAAGA 1 cut(s) 47
MflI RGATCY 3 cut(s) 239, 351, 409
MhlI GDGCHC 2 cut(s) 183, 221
MluCI AATT 4 cut(s) 394, 548, 558, 796
MlyI GAGTC 1 cut(s) 59
MroI TCCGGA 1 cut(s) 604
MroXI GAANNNNTTC 1 cut(s) 562
MseI TTAA 4 cut(s) 276, 525, 696, 996
MslI CAYNNNNRTG 2 cut(s) 156, 663
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 5 cut(s) 209, 305, 356, 605, 1020
MspR9I CCNGG 3 cut(s) 121, 131, 356
MvaI CCWGG 2 cut(s) 121, 131
NciI CCSGG 1 cut(s) 356
NdeII GATC 4 cut(s) 239, 351, 409, 607
NlaIII CATG 2 cut(s) 203, 322
NlaIV GGNNCC 2 cut(s) 353, 886
NmuCI GTSAC 4 cut(s) 46, 263, 307, 373
NspV TTCGAA 1 cut(s) 11
OliI CACNNNNGTG 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 562
PfeI GAWTC 5 cut(s) 13, 19, 25, 618, 1011
PflMI CCANNNNNTGG 1 cut(s) 120
PfoI TCCNGGA 1 cut(s) 354
PinAI ACCGGT 2 cut(s) 208, 304
PkrI GCNGC 4 cut(s) 7, 420, 458, 598
PleI GAGTC 1 cut(s) 59
PmaCI CACGTG 1 cut(s) 976
PmlI CACGTG 1 cut(s) 976
PpsI GAGTC 1 cut(s) 59
Ppu21I YACGTR 1 cut(s) 976
Psp6I CCWGG 2 cut(s) 119, 129
PspCI CACGTG 1 cut(s) 976
PspEI GGTNACC 3 cut(s) 46, 296, 307
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 2 cut(s) 119, 129
PspN4I GGNNCC 2 cut(s) 353, 886
PsrI GAACNNNNNNTAC 2 cut(s) 950, 982
PstI CTGCAG 1 cut(s) 505
PsuI RGATCY 3 cut(s) 239, 351, 409
RsaI GTAC 2 cut(s) 654, 756
RsaNI GTAC 2 cut(s) 653, 755
RseI CAYNNNNRTG 2 cut(s) 156, 663
SaqAI TTAA 4 cut(s) 276, 525, 696, 996
SatI GCNGC 4 cut(s) 6, 419, 457, 597
Sau3AI GATC 4 cut(s) 239, 351, 409, 607
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 3 cut(s) 121, 131, 356
SduI GDGCHC 2 cut(s) 183, 221
SexAI ACCWGGT 1 cut(s) 129
SfaNI GCATC 3 cut(s) 128, 197, 316
SfcI CTRYAG 1 cut(s) 501
SfuI TTCGAA 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 156, 663
Sse9I AATT 4 cut(s) 394, 548, 558, 796
SsiI CCGC 1 cut(s) 774
SspMI CTAG 2 cut(s) 174, 290
StyD4I CCNGG 3 cut(s) 119, 129, 354
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 5 cut(s) 247, 313, 788, 878, 1029
TaiI ACGT 1 cut(s) 978
TaqI TCGA 3 cut(s) 11, 68, 74
TaqII GACCGA 1 cut(s) 522
TasI AATT 4 cut(s) 394, 548, 558, 796
TatI WGTACW 1 cut(s) 652
TfiI GAWTC 5 cut(s) 13, 19, 25, 618, 1011
Tru1I TTAA 4 cut(s) 276, 525, 696, 996
Tru9I TTAA 4 cut(s) 276, 525, 696, 996
TscAI CASTG 4 cut(s) 477, 505, 871, 883
TseFI GTSAC 4 cut(s) 46, 263, 307, 373
TseI GCWGC 4 cut(s) 5, 418, 456, 596
Tsp45I GTSAC 4 cut(s) 46, 263, 307, 373
TspDTI ATGAA 1 cut(s) 377
TspRI CASTG 4 cut(s) 477, 505, 871, 883
Van91I CCANNNNNTGG 1 cut(s) 120
XapI RAATTY 2 cut(s) 394, 548
XmnI GAANNNNTTC 1 cut(s) 562
XspI CTAG 2 cut(s) 174, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.