Rh7AG369300

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001035
Physical Location & Seq
Forward (+)
47590182 .. 47598031
7850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG369300.1

Sequence Viewer

Length: 864 bp
ATGCCCAAGGCAGCTATATCGCACGATAATCCGGTTTTGTGCTCAACTTGGAAAGATGATGGGACAACTGTGTTCTCTGGAGGTTGTGATAAGCAAGTTAAGATGTGGCCTCTGATGTCTGGTGGCAACCAAACAATGACTGTTGCAATGCATGATGCGCCCATCAAGGAAATAGCTTGGATCCCTGAGATGAGCCTCTTGGTGACAGGAAGCTGGGACAAGACTTTGAAGTATTGGGATACAAGGCAGCCAAATCCAGTGCATACCCAACTGCTCCCAGAGAGGTGCTATGCATTATCAGTGAGACACCCTCTGATGGTGGTTGGAACTGCTGATCGAAATCTTATTGTTTATAACTTGCAGAGTCCACAGACTGAGTTCAAGAGAATCATTTCACCCCTAAAGTATCAGACAAGGTGTGTTGCGGCATTTCCTGATCAGCAAGGATTCTTGGTTGGCTCTATTGAAGGGAGGGTTGGTGTACATCATCTGGATGAGGCACAACAAAGTAAAAACTTTACCTTCAAATGCCACAGAGAGGGCAACGAGATATACTCAGTCAATTCTCTGAACTTCCATCCTGTGTATCACACATTTGCTACTGCTGGTTCTGATGGTTCTTTCAACTTTTGGGATAAGGACAGCAAACAGAGACTAAAGGCCATGGCAAGGTGCAGTCAACCAATACCTTGCAGCACTTTCAACAACGATGGTTCCATATTTGCATACTCGGTTTGCTATGATTGGAGTAAGGGCGCAGAAAATCACAATCCGGCCACAGCAAAGAACTACATTTACCTCCATGTTCCACAGGAATCTGAGGTGAAAAGCAAGCCACGAATCTCGACTGGTGGAAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

287

Amino Acids

32.31

Weight (kDa)

8.7

Isoelectric Point (pI)

40.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 7 - 171 4.1e-14 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 7 - 120 8.2e-14 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 7 - 88 3.5e-10 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 8 - 244 4.2e-30 THOC3 beta-propeller domain
WD40_Prp19 PF24814 8 - 88 1.3e-08 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 8 - 86 2.7e-09 WDR3 second beta-propeller domain
WDR55 PF24796 8 - 171 3.4e-12 WDR55
WD40_WDHD1_1st PF24817 9 - 93 3.8e-10 WDHD1 first WD40 domain
WD40 PF00400 44 - 80 6.6e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 59 - 173 2.5e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 94 - 224 1e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 102 - 243 4.6e-13 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 104 - 247 1.4e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 108 - 240 6e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 354
AciI CCGC 1 cut(s) 425
AclWI GGATC 2 cut(s) 175, 188
AcoI YGGCCR 1 cut(s) 774
AfaI GTAC 1 cut(s) 483
AfiI CCNNNNNNNGG 3 cut(s) 316, 538, 669
AgsI TTSAA 6 cut(s) 229, 382, 467, 526, 625, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 459, 491
AluBI AGCT 3 cut(s) 14, 176, 213
AluI AGCT 3 cut(s) 14, 176, 213
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 2 cut(s) 298, 646
AlwI GGATC 2 cut(s) 175, 188
AoxI GGCC 3 cut(s) 107, 660, 774
ApeKI GCWGC 3 cut(s) 11, 247, 693
Asp700I GAANNNNTTC 1 cut(s) 391
AspLEI GCGC 2 cut(s) 160, 758
AsuHPI GGTGA 3 cut(s) 214, 387, 835
BamHI GGATCC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 3 cut(s) 23, 259, 705
BccI CCATC 6 cut(s) 53, 170, 310, 585, 608, 704
BcgI CGANNNNNNTGC 1 cut(s) 34
BciVI GTATCC 1 cut(s) 232
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 2 cut(s) 298, 646
BfuI GTATCC 1 cut(s) 232
BisI GCNGC 4 cut(s) 12, 248, 426, 694
BlsI GCNGC 4 cut(s) 13, 249, 427, 695
BmiI GGNNCC 2 cut(s) 182, 715
BmsI GCATC 1 cut(s) 145
BplI GAGNNNNNCTC 4 cut(s) 295, 327, 539, 571
BpmI CTGGAG 1 cut(s) 99
BsaBI GATNNNNATC 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 6, 663
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 2 cut(s) 463, 493
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 538, 669
Bse1I ACTGG 2 cut(s) 257, 853
Bse3DI GCAATG 1 cut(s) 153
Bse8I GATNNNNATC 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 6, 663
BseGI GGATG 2 cut(s) 499, 577
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 3 cut(s) 316, 538, 669
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 4 cut(s) 177, 366, 570, 810
BseNI ACTGG 2 cut(s) 257, 853
BseXI GCAGC 3 cut(s) 23, 259, 705
BseYI CCCAGC 1 cut(s) 213
BsgI GTGCAG 1 cut(s) 694
BshFI GGCC 3 cut(s) 109, 662, 776
BsiHKAI GWGCWC 1 cut(s) 44
BsiSI CCGG 2 cut(s) 32, 773
BslFI GGGAC 2 cut(s) 76, 230
BslI CCNNNNNNNGG 3 cut(s) 316, 538, 669
BsmAI GTCTC 2 cut(s) 298, 646
BsmFI GGGAC 2 cut(s) 76, 230
BsnI GGCC 3 cut(s) 109, 662, 776
Bsp1286I GDGCHC 1 cut(s) 44
Bsp1407I TGTACA 1 cut(s) 481
Bsp143I GATC 3 cut(s) 180, 334, 436
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 1 cut(s) 425
BspANI GGCC 3 cut(s) 109, 662, 776
BspCNI CTCAG 4 cut(s) 178, 367, 569, 811
BspLI GGNNCC 2 cut(s) 182, 715
BspPI GGATC 2 cut(s) 175, 188
BsrDI GCAATG 1 cut(s) 153
BsrGI TGTACA 1 cut(s) 481
BsrI ACTGG 2 cut(s) 257, 853
BssECI CCNNGG 2 cut(s) 6, 663
BssMI GATC 3 cut(s) 180, 334, 436
BssT1I CCWWGG 2 cut(s) 6, 663
Bst4CI ACNGT 2 cut(s) 70, 142
BstAUI TGTACA 1 cut(s) 481
BstC8I GCNNGC 1 cut(s) 833
BstDEI CTNAG 4 cut(s) 186, 375, 556, 819
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 2 cut(s) 499, 577
BstHHI GCGC 2 cut(s) 160, 758
BstKTI GATC 3 cut(s) 183, 337, 439
BstMAI GTCTC 2 cut(s) 298, 646
BstMBI GATC 3 cut(s) 180, 334, 436
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstV1I GCAGC 3 cut(s) 23, 259, 705
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuI GTATCC 1 cut(s) 232
BsuRI GGCC 3 cut(s) 109, 662, 776
BtgI CCRYGG 1 cut(s) 663
BtsCI GGATG 2 cut(s) 499, 577
BtsIMutI CAGTG 2 cut(s) 264, 306
Cac8I GCNNGC 1 cut(s) 833
CfoI GCGC 2 cut(s) 160, 758
Csp6I GTAC 1 cut(s) 482
CviAII CATG 3 cut(s) 152, 664, 803
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 4 cut(s) 186, 375, 556, 819
DpnI GATC 3 cut(s) 182, 336, 438
DpnII GATC 3 cut(s) 180, 334, 436
EaeI YGGCCR 1 cut(s) 774
Eco130I CCWWGG 2 cut(s) 6, 663
EcoT14I CCWWGG 2 cut(s) 6, 663
EcoT22I ATGCAT 2 cut(s) 153, 295
ErhI CCWWGG 2 cut(s) 6, 663
FaeI CATG 3 cut(s) 155, 667, 806
FaqI GGGAC 2 cut(s) 76, 230
FatI CATG 3 cut(s) 151, 663, 802
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 4 cut(s) 12, 248, 426, 694
FokI GGATG 2 cut(s) 506, 564
Fsp4HI GCNGC 4 cut(s) 12, 248, 426, 694
GlaI GCGC 2 cut(s) 159, 757
GluI GCNGC 4 cut(s) 12, 248, 426, 694
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 99
HaeIII GGCC 3 cut(s) 109, 662, 776
HapII CCGG 2 cut(s) 32, 773
HhaI GCGC 2 cut(s) 160, 758
Hin1II CATG 3 cut(s) 155, 667, 806
Hin6I GCGC 2 cut(s) 158, 756
HinP1I GCGC 2 cut(s) 158, 756
HincII GTYRAC 1 cut(s) 680
HindII GTYRAC 1 cut(s) 680
HinfI GANTC 5 cut(s) 364, 387, 447, 815, 840
HpaII CCGG 2 cut(s) 32, 773
HphI GGTGA 3 cut(s) 214, 387, 835
Hpy166II GTNNAC 3 cut(s) 368, 482, 680
Hpy188I TCNGA 6 cut(s) 114, 315, 411, 570, 613, 820
Hpy188III TCNNGA 5 cut(s) 78, 382, 434, 491, 844
Hpy8I GTNNAC 3 cut(s) 368, 482, 680
HpyAV CCTTC 2 cut(s) 461, 532
HpyCH4III ACNGT 2 cut(s) 70, 142
HpyCH4V TGCA 8 cut(s) 146, 151, 262, 293, 361, 675, 693, 725
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 4 cut(s) 186, 375, 556, 819
Hsp92II CATG 3 cut(s) 155, 667, 806
HspAI GCGC 2 cut(s) 158, 756
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 3 cut(s) 180, 334, 436
LmnI GCTCC 1 cut(s) 279
Lsp1109I GCAGC 3 cut(s) 23, 259, 705
LweI GCATC 1 cut(s) 145
MaeIII GTNAC 1 cut(s) 202
MalI GATC 3 cut(s) 182, 336, 438
MboI GATC 3 cut(s) 180, 334, 436
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 44
MluCI AATT 1 cut(s) 562
MlyI GAGTC 1 cut(s) 373
MmeI TCCRAC 1 cut(s) 304
Mph1103I ATGCAT 2 cut(s) 153, 295
MroXI GAANNNNTTC 1 cut(s) 391
MseI TTAA 1 cut(s) 99
MslI CAYNNNNRTG 1 cut(s) 492
MspI CCGG 2 cut(s) 32, 773
MwoI GCNNNNNNNGC 1 cut(s) 157
NcoI CCATGG 1 cut(s) 663
NdeII GATC 3 cut(s) 180, 334, 436
NlaIII CATG 3 cut(s) 155, 667, 806
NlaIV GGNNCC 2 cut(s) 182, 715
NmuCI GTSAC 1 cut(s) 202
NsiI ATGCAT 2 cut(s) 153, 295
PdmI GAANNNNTTC 1 cut(s) 391
PfeI GAWTC 4 cut(s) 387, 447, 815, 840
PkrI GCNGC 4 cut(s) 13, 249, 427, 695
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
PsiI TTATAA 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 213
PspN4I GGNNCC 2 cut(s) 182, 715
PsrI GAACNNNNNNTAC 2 cut(s) 779, 811
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 492
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 4 cut(s) 12, 248, 426, 694
Sau3AI GATC 3 cut(s) 180, 334, 436
SchI GAGTC 1 cut(s) 373
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 492
Sse9I AATT 1 cut(s) 562
SsiI CCGC 1 cut(s) 425
StyI CCWWGG 2 cut(s) 6, 663
TaaI ACNGT 2 cut(s) 70, 142
TaqI TCGA 2 cut(s) 337, 845
TasI AATT 1 cut(s) 562
TatI WGTACW 1 cut(s) 481
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 4 cut(s) 387, 447, 815, 840
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 2 cut(s) 264, 306
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 3 cut(s) 11, 247, 693
Tsp45I GTSAC 1 cut(s) 202
TspRI CASTG 2 cut(s) 264, 306
XmnI GAANNNNTTC 1 cut(s) 391
Zsp2I ATGCAT 2 cut(s) 153, 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.