Rh7CG093200

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001035
Physical Location & Seq
Reverse (-)
7021688 .. 7024889
3202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG093200.1

Sequence Viewer

Length: 711 bp
ATGGCTGTATTCTCTGGAGGTTGTGACAGGCAAGTTAAGATGTGGTCTCTAATGTCTGGTGGCCAAACGAGTACTGTTGCCATGCATGATGCACCCATAAAAGAGATTGCTTGGATTCCCGAGATGGCGCTCTTAGTCACAGGCAGCTGGGACAAGACTATGAAGTACTGGGATTTAAGGCAGCCAAATCCAGTGCATACCCAACAACTACCCGAACGCTGTTATGCACTAACAGTGAAACACCCTCTAATGGTTGTTGGAACAGCAGATAGAAATCTGATCATATTTAACTTGCAGAGTCCACAGGTTGGTTCAATTGAAGGGAGGGTTGGAGTGCAGCATCTGGATGAAGGACAAGATAGTAAAAACTTTACCTTCAAATGCCACAGAGAAAACAATGCTCTTTACTCAGTCAACTCTATAAACTTCCATCCTGTGCATCACACATTTTCTACAGCTGGTTCTGATGGTTCTATCAGTTTTTGGGATAAGGACAGCAAACAGAGACTCAAGGCCATGCAAAGGTGCAATCAACCCATTCCTTGCAGTACTTTCAACAATGATGGTTCCATATATGCATACTCTGTTTGCTATGACTGGCATAAGGGAGCAGAAAATCATAATCCTTCTACTGCAAAGACCTCCATTTTCCTCCACTTGCCACAGGACGCTGATGTTGAAAGCAAGCCAAGACCTGGTGGAAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

236

Amino Acids

26.52

Weight (kDa)

8.2

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 3 - 102 2.7e-09 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 3 - 97 9.5e-12 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 3 - 76 8e-09 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 3 - 96 2.1e-07 WDR3 second beta-propeller domain
EIF3I PF24805 3 - 190 3.7e-08 EIF3I
Beta-prop_WDR5 PF25175 4 - 100 7.2e-10 WDR5 beta-propeller domain
WD40_Gbeta PF25391 4 - 61 8.7e-06 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 5 - 102 3.1e-12 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 5 - 68 6.9e-07 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 108 - 194 1.6e-07 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 114 - 194 4.8e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 308, 695
AcoI YGGCCR 1 cut(s) 61
AfaI GTAC 3 cut(s) 73, 167, 550
AfiI CCNNNNNNNGG 4 cut(s) 250, 308, 522, 695
AgsI TTSAA 5 cut(s) 315, 320, 379, 556, 680
AjnI CCWGG 1 cut(s) 694
AjuI GAANNNNNNNTTGG 2 cut(s) 312, 344
AluBI AGCT 2 cut(s) 147, 458
AluI AGCT 2 cut(s) 147, 458
Alw26I GTCTC 2 cut(s) 51, 499
AlwNI CAGNNNCTG 1 cut(s) 343
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 2 cut(s) 61, 513
ApeKI GCWGC 3 cut(s) 144, 181, 337
AspLEI GCGC 1 cut(s) 130
AvaI CYCGRG 1 cut(s) 119
BalI TGGCCA 1 cut(s) 63
BbvI GCAGC 3 cut(s) 156, 193, 349
BccI CCATC 4 cut(s) 118, 438, 461, 557
BciT130I CCWGG 1 cut(s) 696
BclI TGATCA 1 cut(s) 279
BcoDI GTCTC 2 cut(s) 51, 499
BfmI CTRYAG 1 cut(s) 453
BfoI RGCGCY 1 cut(s) 131
BisI GCNGC 3 cut(s) 145, 182, 338
BlsI GCNGC 3 cut(s) 146, 183, 339
BmcAI AGTACT 3 cut(s) 73, 167, 550
Bme1390I CCNGG 1 cut(s) 696
BmeT110I CYCGRG 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 568
BmrFI CCNGG 1 cut(s) 696
BmrI ACTGGG 1 cut(s) 178
BmsI GCATC 3 cut(s) 79, 349, 448
BmuI ACTGGG 1 cut(s) 178
BpmI CTGGAG 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 494
BsaBI GATNNNNATC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 51
Bsc4I CCNNNNNNNGG 4 cut(s) 250, 308, 522, 695
Bse1I ACTGG 3 cut(s) 173, 191, 602
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 1 cut(s) 696
BseGI GGATG 2 cut(s) 352, 430
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 4 cut(s) 250, 308, 522, 695
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 3 cut(s) 173, 191, 602
BseXI GCAGC 3 cut(s) 156, 193, 349
BseYI CCCAGC 1 cut(s) 147
BsgI GTGCAG 1 cut(s) 356
BshFI GGCC 2 cut(s) 63, 515
BsiHKCI CYCGRG 1 cut(s) 119
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 4 cut(s) 250, 308, 522, 695
BsmAI GTCTC 2 cut(s) 51, 499
BsmFI GGGAC 1 cut(s) 164
BsnI GGCC 2 cut(s) 63, 515
Bso31I GGTCTC 1 cut(s) 51
BsoBI CYCGRG 1 cut(s) 119
Bsp143I GATC 1 cut(s) 279
BspANI GGCC 2 cut(s) 63, 515
BspCNI CTCAG 1 cut(s) 422
BspLI GGNNCC 1 cut(s) 568
BspTNI GGTCTC 1 cut(s) 51
BsrI ACTGG 3 cut(s) 173, 191, 602
BssMI GATC 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 696
Bst4CI ACNGT 2 cut(s) 76, 235
BstC8I GCNNGC 1 cut(s) 686
BstDEI CTNAG 2 cut(s) 133, 409
BstF5I GGATG 2 cut(s) 352, 430
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 1 cut(s) 282
BstMAI GTCTC 2 cut(s) 51, 499
BstMBI GATC 1 cut(s) 279
BstNI CCWGG 1 cut(s) 696
BstSCI CCNGG 1 cut(s) 694
BstSFI CTRYAG 1 cut(s) 453
BstV1I GCAGC 3 cut(s) 156, 193, 349
BsuRI GGCC 2 cut(s) 63, 515
BtsCI GGATG 2 cut(s) 352, 430
BtsIMutI CAGTG 2 cut(s) 198, 240
Cac8I GCNNGC 1 cut(s) 686
CaiI CAGNNNCTG 1 cut(s) 343
CfoI GCGC 1 cut(s) 130
CseI GACGC 1 cut(s) 677
CsiI ACCWGGT 1 cut(s) 694
Csp6I GTAC 3 cut(s) 72, 166, 549
CviAII CATG 3 cut(s) 82, 86, 517
CviJI RGCY 7 cut(s) 5, 63, 147, 184, 458, 515, 688
CviKI_1 RGCY 7 cut(s) 5, 63, 147, 184, 458, 515, 688
CviQI GTAC 3 cut(s) 72, 166, 549
DdeI CTNAG 2 cut(s) 133, 409
DpnI GATC 1 cut(s) 281
DpnII GATC 1 cut(s) 279
EaeI YGGCCR 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 119
EcoRII CCWGG 1 cut(s) 694
EcoT22I ATGCAT 2 cut(s) 87, 580
FaeI CATG 3 cut(s) 85, 89, 520
FaqI GGGAC 1 cut(s) 164
FatI CATG 3 cut(s) 81, 85, 516
FbaI TGATCA 1 cut(s) 279
Fnu4HI GCNGC 3 cut(s) 145, 182, 338
FokI GGATG 2 cut(s) 359, 417
Fsp4HI GCNGC 3 cut(s) 145, 182, 338
GlaI GCGC 1 cut(s) 129
GluI GCNGC 3 cut(s) 145, 182, 338
GsaI CCCAGC 1 cut(s) 151
GsuI CTGGAG 1 cut(s) 36
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 2 cut(s) 63, 515
HgaI GACGC 1 cut(s) 677
HhaI GCGC 1 cut(s) 130
Hin1II CATG 3 cut(s) 85, 89, 520
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 3 cut(s) 115, 298, 507
Hpy166II GTNNAC 2 cut(s) 302, 415
Hpy188I TCNGA 2 cut(s) 279, 466
Hpy188III TCNNGA 3 cut(s) 15, 119, 344
Hpy8I GTNNAC 2 cut(s) 302, 415
HpyAV CCTTC 5 cut(s) 314, 344, 385, 636, 699
HpyCH4III ACNGT 2 cut(s) 76, 235
HpyF3I CTNAG 2 cut(s) 133, 409
Hsp92II CATG 3 cut(s) 85, 89, 520
HspAI GCGC 1 cut(s) 128
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 1 cut(s) 279
LmnI GCTCC 1 cut(s) 608
Lsp1109I GCAGC 3 cut(s) 156, 193, 349
LweI GCATC 3 cut(s) 79, 349, 448
MabI ACCWGGT 1 cut(s) 694
MaeIII GTNAC 2 cut(s) 23, 136
MalI GATC 1 cut(s) 281
MboI GATC 1 cut(s) 279
MfeI CAATTG 1 cut(s) 315
MlsI TGGCCA 1 cut(s) 63
MluCI AATT 1 cut(s) 315
MluNI TGGCCA 1 cut(s) 63
MlyI GAGTC 2 cut(s) 307, 501
MmeI TCCRAC 2 cut(s) 238, 310
MnlI CCTC 5 cut(s) 11, 255, 318, 652, 662
Mox20I TGGCCA 1 cut(s) 63
Mph1103I ATGCAT 2 cut(s) 87, 580
MscI TGGCCA 1 cut(s) 63
MseI TTAA 3 cut(s) 36, 176, 288
MslI CAYNNNNRTG 1 cut(s) 345
Msp20I TGGCCA 1 cut(s) 63
MspA1I CMGCKG 2 cut(s) 147, 458
MspR9I CCNGG 1 cut(s) 696
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 696
NdeII GATC 1 cut(s) 279
NlaIII CATG 3 cut(s) 85, 89, 520
NlaIV GGNNCC 1 cut(s) 568
NmuCI GTSAC 2 cut(s) 23, 136
NsiI ATGCAT 2 cut(s) 87, 580
PfeI GAWTC 1 cut(s) 115
PflMI CCANNNNNTGG 2 cut(s) 308, 695
PkrI GCNGC 3 cut(s) 146, 183, 339
PleI GAGTC 2 cut(s) 306, 501
PpsI GAGTC 2 cut(s) 306, 501
Psp6I CCWGG 1 cut(s) 694
PspFI CCCAGC 1 cut(s) 147
PspGI CCWGG 1 cut(s) 694
PspN4I GGNNCC 1 cut(s) 568
PstNI CAGNNNCTG 1 cut(s) 343
PvuII CAGCTG 2 cut(s) 147, 458
RsaI GTAC 3 cut(s) 73, 167, 550
RsaNI GTAC 3 cut(s) 72, 166, 549
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 3 cut(s) 36, 176, 288
SatI GCNGC 3 cut(s) 145, 182, 338
Sau3AI GATC 1 cut(s) 279
ScaI AGTACT 3 cut(s) 73, 167, 550
SchI GAGTC 2 cut(s) 307, 501
ScrFI CCNGG 1 cut(s) 696
SetI ASST 9 cut(s) 22, 149, 309, 377, 460, 527, 644, 697, 710
SexAI ACCWGGT 1 cut(s) 694
SfaNI GCATC 3 cut(s) 79, 349, 448
SfcI CTRYAG 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 694
TaaI ACNGT 2 cut(s) 76, 235
TasI AATT 1 cut(s) 315
TatI WGTACW 3 cut(s) 71, 165, 548
TfiI GAWTC 1 cut(s) 115
Tru1I TTAA 3 cut(s) 36, 176, 288
Tru9I TTAA 3 cut(s) 36, 176, 288
TscAI CASTG 2 cut(s) 198, 240
TseFI GTSAC 2 cut(s) 23, 136
TseI GCWGC 3 cut(s) 144, 181, 337
Tsp45I GTSAC 2 cut(s) 23, 136
TspDTI ATGAA 2 cut(s) 176, 363
TspRI CASTG 2 cut(s) 198, 240
Van91I CCANNNNNTGG 2 cut(s) 308, 695
ZrmI AGTACT 3 cut(s) 73, 167, 550
Zsp2I ATGCAT 2 cut(s) 87, 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.