MD08G1221600.v1.1

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001035
Physical Location & Seq
Forward (+)
28411242 .. 28416688
5447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1221600.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGTCGAATTTCATGGCTGCTTCCAATCCGAATACGAATCCGAACAAGTCATTTGAGGTCACCCCTCCACCGAACGACTCGATATCAAGCCTAAGCTTCAGTCCCAAGAGCAACATTCTGGTCGCCACCTCCTGGGATAACCAGGTGCGATGCTGGGAGATACACCAGAGTGGACAAAATCTTGCTAGTGTGCCCAAGGCATCCATATCCCATGACCAACCGGTTTTGTGCTCAACCTGGAAGGATGATGGATCGACAGTGTTCTCCGGAGGGTGCGACAAACAAGTTAAGATGTGGCCTTTGGCTGGTAACCAACCAGTGACCGTTGCCATGCATGAAGCACCCATAAAAGAGATTGCATGGATCCCGGAGATGAACTACTTAGTCACAGGAAGCTGGGACAAGACTTTGAAGTATTGGGATCTGAGGCAGCAAACTCCAATCCATACCCAACCTCTACCTGAGCGCTGCTACACTCTATCAGTGAGATACCCTCTAATGGTTGTTGGCACTGCAGACAGAAATCTGATTGTTTTTAACTTGCAAAACCCACAGACAGAATTCAAGAGAATTGTTTCACCCCTAAAGTATCAAACAAGGTGTGTTGCTGCATTTCCTGATCAGCAAGGATTCTTGGTTGGTTCTATTGAAGGGAGGGTTGGTGTACATCATCTGGATGATGGACTACAAAGTAAAAACTTTACCTTCAAGTGCCATAGAGAGGGCAATGAGATATACTCAGTCAACTCTCTGAACTTCCATCCAGTCCATCACACATTTGCTACCGCTGGTTCTGACGGTTCTTTCAATTTTTGGGATAAGGATAGCAAACAGAGACTAAAGGCAATGGCAAGGTGCAGTCAACCTATTCCTTGCAGTGCTTTCAACAGTGATGGTTCCATATTTGCATACGCAGTTTGCTATGATTGGAGCAAGGGCGCTGAAAATCATAATCCTGCTACTGCAAAGAACTACATTTACCTCCATGTGCCCCAGGAAGCTGAGGTTAAAGGCAAGCCAAGAATCAACGCTGGAAAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

348

Amino Acids

38.85

Weight (kDa)

8.49

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 21 - 150 5.2e-14 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 24 - 160 7.3e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 25 - 174 2.5e-11 WDR3 second beta-propeller domain
WDR55 PF24796 25 - 226 2.3e-17 WDR55
Beta-prop_WDR3_1st PF25173 26 - 180 2.4e-19 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 27 - 155 1.5e-13 Prp19 WD40 domain
Beta-prop_Aladin PF25460 27 - 186 2e-06 Aladin seven-bladed propeller
WD40_Gbeta PF25391 37 - 145 1.4e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 70 - 231 1.8e-12 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 71 - 305 2.2e-29 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 72 - 153 9.4e-12 WDHD1 first WD40 domain
WD40 PF00400 110 - 141 6.1e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 120 - 232 2e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 156 - 285 7.6e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 166 - 305 9.3e-13 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 169 - 300 2.2e-06 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 250 - 305 5e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 132
AccIII TCCGGA 1 cut(s) 266
AciI CCGC 1 cut(s) 786
AclWI GGATC 4 cut(s) 259, 358, 371, 429
AcsI RAATTY 2 cut(s) 7, 560
AcuI CTGAAG 1 cut(s) 82
AfaI GTAC 1 cut(s) 666
AfeI AGCGCT 1 cut(s) 467
AfiI CCNNNNNNNGG 4 cut(s) 132, 305, 499, 721
AgeI ACCGGT 1 cut(s) 220
AgsI TTSAA 6 cut(s) 412, 565, 650, 709, 808, 886
AjnI CCWGG 4 cut(s) 131, 141, 236, 993
AjuI GAANNNNNNNTTGG 2 cut(s) 642, 674
AleI CACNNNNGTG 1 cut(s) 168
AluBI AGCT 3 cut(s) 96, 396, 1001
AluI AGCT 3 cut(s) 96, 396, 1001
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 1 cut(s) 829
AlwI GGATC 4 cut(s) 259, 358, 371, 429
Aor13HI TCCGGA 1 cut(s) 266
Aor51HI AGCGCT 1 cut(s) 467
AoxI GGCC 1 cut(s) 296
ApeKI GCWGC 4 cut(s) 17, 430, 468, 608
ApoI RAATTY 2 cut(s) 7, 560
AsiGI ACCGGT 1 cut(s) 220
Asp700I GAANNNNTTC 1 cut(s) 574
AspLEI GCGC 2 cut(s) 468, 941
AsuC2I CCSGG 1 cut(s) 368
AsuHPI GGTGA 2 cut(s) 52, 570
BaeGI GKGCMC 2 cut(s) 195, 993
BamHI GGATCC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 233
BbvCI CCTCAGC 1 cut(s) 1002
BbvI GCAGC 4 cut(s) 4, 442, 455, 595
BccI CCATC 5 cut(s) 242, 674, 768, 777, 887
BciT130I CCWGG 4 cut(s) 133, 143, 238, 995
BclI TGATCA 1 cut(s) 619
BcnI CCSGG 1 cut(s) 368
BcoDI GTCTC 1 cut(s) 829
BfaI CTAG 1 cut(s) 186
BfmI CTRYAG 1 cut(s) 513
BfoI RGCGCY 2 cut(s) 469, 942
BisI GCNGC 4 cut(s) 18, 431, 469, 609
BlsI GCNGC 4 cut(s) 19, 432, 470, 610
Bme1390I CCNGG 5 cut(s) 133, 143, 238, 368, 995
BmiI GGNNCC 2 cut(s) 365, 898
BmrFI CCNGG 5 cut(s) 133, 143, 238, 368, 995
BmsI GCATC 2 cut(s) 140, 209
BplI GAGNNNNNCTC 4 cut(s) 478, 510, 722, 754
Bpu10I CCTNAGC 3 cut(s) 92, 462, 1002
BpuMI CCSGG 1 cut(s) 368
BsaJI CCNNGG 3 cut(s) 132, 195, 993
BsaWI WCCGGW 2 cut(s) 220, 266
BsaXI ACNNNNNCTCC 2 cut(s) 646, 676
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 305, 499, 721
Bse118I RCCGGY 1 cut(s) 220
Bse1I ACTGG 2 cut(s) 317, 764
Bse3DI GCAATG 2 cut(s) 733, 852
BseAI TCCGGA 1 cut(s) 266
BseBI CCWGG 4 cut(s) 133, 143, 238, 995
BseDI CCNNGG 3 cut(s) 132, 195, 993
BseGI GGATG 4 cut(s) 200, 250, 682, 760
BseLI CCNNNNNNNGG 4 cut(s) 132, 305, 499, 721
BseMI GCAATG 2 cut(s) 733, 852
BseMII CTCAG 4 cut(s) 416, 453, 753, 993
BseNI ACTGG 2 cut(s) 317, 764
BseSI GKGCMC 2 cut(s) 195, 993
BseXI GCAGC 4 cut(s) 4, 442, 455, 595
BseYI CCCAGC 2 cut(s) 153, 396
BsgI GTGCAG 1 cut(s) 877
BshFI GGCC 1 cut(s) 298
BshTI ACCGGT 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 233
BsiSI CCGG 3 cut(s) 221, 267, 368
BslFI GGGAC 2 cut(s) 87, 413
BslI CCNNNNNNNGG 4 cut(s) 132, 305, 499, 721
BsmAI GTCTC 1 cut(s) 829
BsmFI GGGAC 2 cut(s) 87, 413
BsnI GGCC 1 cut(s) 298
Bsp1286I GDGCHC 3 cut(s) 195, 233, 993
Bsp13I TCCGGA 1 cut(s) 266
Bsp1407I TGTACA 1 cut(s) 664
Bsp143I GATC 4 cut(s) 251, 363, 421, 619
BspACI CCGC 1 cut(s) 786
BspANI GGCC 1 cut(s) 298
BspCNI CTCAG 4 cut(s) 417, 454, 752, 994
BspEI TCCGGA 1 cut(s) 266
BspLI GGNNCC 2 cut(s) 365, 898
BspMAI CTGCAG 1 cut(s) 517
BspPI GGATC 4 cut(s) 259, 358, 371, 429
BsrDI GCAATG 2 cut(s) 733, 852
BsrFI RCCGGY 1 cut(s) 220
BsrGI TGTACA 1 cut(s) 664
BsrI ACTGG 2 cut(s) 317, 764
BssAI RCCGGY 1 cut(s) 220
BssECI CCNNGG 3 cut(s) 132, 195, 993
BssMI GATC 4 cut(s) 251, 363, 421, 619
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 4 cut(s) 133, 143, 238, 995
Bst4CI ACNGT 5 cut(s) 259, 325, 800, 890, 1041
BstAUI TGTACA 1 cut(s) 664
BstC8I GCNNGC 1 cut(s) 1016
BstDEI CTNAG 6 cut(s) 92, 382, 425, 462, 739, 1002
BstEII GGTNACC 2 cut(s) 58, 308
BstF5I GGATG 4 cut(s) 200, 250, 682, 760
BstH2I RGCGCY 2 cut(s) 469, 942
BstHHI GCGC 2 cut(s) 468, 941
BstKTI GATC 4 cut(s) 254, 366, 424, 622
BstMAI GTCTC 1 cut(s) 829
BstMBI GATC 4 cut(s) 251, 363, 421, 619
BstNI CCWGG 4 cut(s) 133, 143, 238, 995
BstPI GGTNACC 2 cut(s) 58, 308
BstSCI CCNGG 5 cut(s) 131, 141, 236, 366, 993
BstSFI CTRYAG 1 cut(s) 513
BstSLI GKGCMC 2 cut(s) 195, 993
BstV1I GCAGC 4 cut(s) 4, 442, 455, 595
BstX2I RGATCY 2 cut(s) 363, 421
BstYI RGATCY 2 cut(s) 363, 421
BsuRI GGCC 1 cut(s) 298
BtgZI GCGATG 1 cut(s) 163
BtsCI GGATG 4 cut(s) 200, 250, 682, 760
BtsI GCAGTG 2 cut(s) 510, 883
BtsIMutI CAGTG 6 cut(s) 264, 324, 489, 510, 883, 895
Cac8I GCNNGC 1 cut(s) 1016
CfoI GCGC 2 cut(s) 468, 941
Cfr10I RCCGGY 1 cut(s) 220
CsiI ACCWGGT 1 cut(s) 141
Csp6I GTAC 1 cut(s) 665
CspAI ACCGGT 1 cut(s) 220
CviAII CATG 6 cut(s) 13, 212, 331, 335, 360, 986
CviJI RGCY 8 cut(s) 17, 90, 96, 298, 305, 396, 1001, 1018
CviKI_1 RGCY 8 cut(s) 17, 90, 96, 298, 305, 396, 1001, 1018
CviQI GTAC 1 cut(s) 665
DdeI CTNAG 6 cut(s) 92, 382, 425, 462, 739, 1002
DpnI GATC 4 cut(s) 253, 365, 423, 621
DpnII GATC 4 cut(s) 251, 363, 421, 619
Eco130I CCWWGG 1 cut(s) 195
Eco32I GATATC 1 cut(s) 84
Eco47III AGCGCT 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 82
Eco91I GGTNACC 2 cut(s) 58, 308
EcoO65I GGTNACC 2 cut(s) 58, 308
EcoRI GAATTC 1 cut(s) 560
EcoRII CCWGG 4 cut(s) 131, 141, 236, 993
EcoRV GATATC 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 6 cut(s) 16, 215, 334, 338, 363, 989
FaqI GGGAC 2 cut(s) 87, 413
FatI CATG 6 cut(s) 12, 211, 330, 334, 359, 985
FbaI TGATCA 1 cut(s) 619
Fnu4HI GCNGC 4 cut(s) 18, 431, 469, 609
FokI GGATG 4 cut(s) 187, 257, 689, 747
Fsp4HI GCNGC 4 cut(s) 18, 431, 469, 609
FspBI CTAG 1 cut(s) 186
GlaI GCGC 2 cut(s) 467, 940
GluI GCNGC 4 cut(s) 18, 431, 469, 609
GsaI CCCAGC 2 cut(s) 157, 400
HaeII RGCGCY 2 cut(s) 469, 942
HaeIII GGCC 1 cut(s) 298
HapII CCGG 3 cut(s) 221, 267, 368
HhaI GCGC 2 cut(s) 468, 941
Hin1II CATG 6 cut(s) 16, 215, 334, 338, 363, 989
Hin6I GCGC 2 cut(s) 466, 939
HinP1I GCGC 2 cut(s) 466, 939
HincII GTYRAC 2 cut(s) 745, 863
HindII GTYRAC 2 cut(s) 745, 863
HindIII AAGCTT 1 cut(s) 94
HinfI GANTC 4 cut(s) 37, 77, 630, 1023
HpaII CCGG 3 cut(s) 221, 267, 368
HphI GGTGA 2 cut(s) 52, 570
Hpy166II GTNNAC 4 cut(s) 173, 665, 745, 863
Hpy188I TCNGA 6 cut(s) 30, 42, 426, 528, 753, 796
Hpy188III TCNNGA 4 cut(s) 267, 565, 617, 674
Hpy8I GTNNAC 4 cut(s) 173, 665, 745, 863
HpyAV CCTTC 3 cut(s) 235, 644, 715
HpyCH4III ACNGT 5 cut(s) 259, 325, 800, 890, 1041
HpyCH4V TGCA 9 cut(s) 334, 359, 515, 544, 611, 858, 876, 908, 965
HpyF3I CTNAG 6 cut(s) 92, 382, 425, 462, 739, 1002
Hsp92II CATG 6 cut(s) 16, 215, 334, 338, 363, 989
HspAI GCGC 2 cut(s) 466, 939
Kpn2I TCCGGA 1 cut(s) 266
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 4 cut(s) 251, 363, 421, 619
LmnI GCTCC 1 cut(s) 930
Lsp1109I GCAGC 4 cut(s) 4, 442, 455, 595
LweI GCATC 2 cut(s) 140, 209
MabI ACCWGGT 1 cut(s) 141
MaeI CTAG 1 cut(s) 186
MaeIII GTNAC 4 cut(s) 58, 308, 319, 385
MalI GATC 4 cut(s) 253, 365, 423, 621
MboI GATC 4 cut(s) 251, 363, 421, 619
MflI RGATCY 2 cut(s) 363, 421
MhlI GDGCHC 3 cut(s) 195, 233, 993
MluCI AATT 4 cut(s) 7, 560, 570, 808
MlyI GAGTC 1 cut(s) 71
Mph1103I ATGCAT 1 cut(s) 336
MroI TCCGGA 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 574
MseI TTAA 3 cut(s) 288, 537, 1008
MslI CAYNNNNRTG 2 cut(s) 168, 675
MspA1I CMGCKG 1 cut(s) 788
MspI CCGG 3 cut(s) 221, 267, 368
MspR9I CCNGG 5 cut(s) 133, 143, 238, 368, 995
MvaI CCWGG 4 cut(s) 133, 143, 238, 995
NciI CCSGG 1 cut(s) 368
NdeII GATC 4 cut(s) 251, 363, 421, 619
NlaIII CATG 6 cut(s) 16, 215, 334, 338, 363, 989
NlaIV GGNNCC 2 cut(s) 365, 898
NmuCI GTSAC 3 cut(s) 58, 319, 385
NsiI ATGCAT 1 cut(s) 336
OliI CACNNNNGTG 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 574
PfeI GAWTC 3 cut(s) 37, 630, 1023
PflMI CCANNNNNTGG 1 cut(s) 132
PfoI TCCNGGA 1 cut(s) 366
PinAI ACCGGT 1 cut(s) 220
PkrI GCNGC 4 cut(s) 19, 432, 470, 610
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
Psp6I CCWGG 4 cut(s) 131, 141, 236, 993
PspEI GGTNACC 2 cut(s) 58, 308
PspFI CCCAGC 2 cut(s) 153, 396
PspGI CCWGG 4 cut(s) 131, 141, 236, 993
PspN4I GGNNCC 2 cut(s) 365, 898
PsrI GAACNNNNNNTAC 2 cut(s) 962, 994
PstI CTGCAG 1 cut(s) 517
PsuI RGATCY 2 cut(s) 363, 421
RsaI GTAC 1 cut(s) 666
RsaNI GTAC 1 cut(s) 665
RseI CAYNNNNRTG 2 cut(s) 168, 675
SaqAI TTAA 3 cut(s) 288, 537, 1008
SatI GCNGC 4 cut(s) 18, 431, 469, 609
Sau3AI GATC 4 cut(s) 251, 363, 421, 619
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 5 cut(s) 133, 143, 238, 368, 995
SduI GDGCHC 3 cut(s) 195, 233, 993
SexAI ACCWGGT 1 cut(s) 141
SfaNI GCATC 2 cut(s) 140, 209
SfcI CTRYAG 1 cut(s) 513
SmiMI CAYNNNNRTG 2 cut(s) 168, 675
Sse9I AATT 4 cut(s) 7, 560, 570, 808
SsiI CCGC 1 cut(s) 786
SspMI CTAG 1 cut(s) 186
StyD4I CCNGG 5 cut(s) 131, 141, 236, 366, 993
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 5 cut(s) 259, 325, 800, 890, 1041
TaqI TCGA 3 cut(s) 5, 80, 254
TasI AATT 4 cut(s) 7, 560, 570, 808
TatI WGTACW 1 cut(s) 664
TfiI GAWTC 3 cut(s) 37, 630, 1023
Tru1I TTAA 3 cut(s) 288, 537, 1008
Tru9I TTAA 3 cut(s) 288, 537, 1008
TscAI CASTG 6 cut(s) 264, 324, 489, 517, 883, 895
TseFI GTSAC 3 cut(s) 58, 319, 385
TseI GCWGC 4 cut(s) 17, 430, 468, 608
Tsp45I GTSAC 3 cut(s) 58, 319, 385
TspDTI ATGAA 2 cut(s) 351, 389
TspRI CASTG 6 cut(s) 264, 324, 489, 517, 883, 895
Van91I CCANNNNNTGG 1 cut(s) 132
XapI RAATTY 2 cut(s) 7, 560
XmnI GAANNNNTTC 1 cut(s) 574
XspI CTAG 1 cut(s) 186
Zsp2I ATGCAT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.