Rw2G020030

Rae1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001035
Physical Location & Seq
Reverse (-)
27118981 .. 27122990
4010 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020030.1

Sequence Viewer

Length: 1029 bp
ATGTCGAGTTTCATGCCGGCCGGCAATCAGAACACGAATCCCAACAAGTCCTTCGAGGTCGCCGAACCTCCAAACGACTCCATTTCGAGCCTGAGCTTCAGCCCCAAAGCCAATCACCTCGTCGCCACCTCCTGGGACAACCAGGTTCGATGCTGGGAGATACAACACAGTGGACAAAATCTTGCTAGCACCCCCAAGGGAATGATTTCACACGACCATCCGGCTTTGTGCTCAACTTGGAAGGATGATGGGGCGACTGTCTTCTCTGGAGGATGTGACAAGCAAGCTAAGATGTGGTCCTTGGCTACTAACCAAACAATGACTGTTGCCGTGCATGATGCCCCCATTAAAGACATGGCTTGGATCCCCGAGTTGAACCTCCTAGTCACTGGAAGCTTGGACAAGACTGTAAAGTATTGGGATACGAGGCAGCAGAGCCCAGCGCACACCCAACAGCTCCCTGAACGCTGCTATGCACTATCAGTGAAACAGTCTCTAATGGTTGTTGGGACGGCGGATAGAAATATTATTATTTTTCACTTGCAGCAACCCCAGAATTGTTTCACCCTAAAGTTTCAGACAAGGTGTGTTGCTGCATTCCCTGATCAGCAAGGATTCTTGGTTGGATCTATTGAGGGGAGGGTTGGTGTGCATCATCTGGATGAGCAACAAGTATCTAAAAACTTTACCTTCAAATGCCACAGGGAGGGCAATGAGATATACTCTGTCAACTCTCTGAACTTCCATCCTGTCCACCACACGTTTGCTACCGCTGGCTCTGATGGTTCTTTTAATTTTTGGGACAAGGACAGCAAACAAAGACTCAAGGCAATGCAAAGGTGCAGTCAGCCTATACCTTGCAGTACCTTTAACCACGATGGTTCCATATATGCATATGCGGTTTGCTACGATTGGAGCAAGGGTGCAGAAAATCATAATCCTGCGGCAGCAAAGAACCACATTTTCCTCCATTTGCCACAGGAAGCTGAGGTAAAAGCCAAGCCGCGAGTTTCAACTGGAAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
WD40

Protein Analysis

342

Amino Acids

38.17

Weight (kDa)

8.03

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 19 - 149 1.6e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 20 - 180 2.6e-19 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 22 - 176 1.7e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 25 - 230 4.2e-21 WDR5 beta-propeller domain
WDR55 PF24796 25 - 223 8.9e-16 WDR55
Beta-prop_WDR3_2nd PF25172 26 - 144 5e-08 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 27 - 186 1.5e-06 Aladin seven-bladed propeller
WD40_Gbeta PF25391 37 - 144 9.9e-08 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 73 - 155 3e-09 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 78 - 300 3.3e-30 THOC3 beta-propeller domain
WD40_Prp19 PF24814 81 - 151 2e-09 Prp19 WD40 domain
WD40 PF00400 106 - 141 4.5e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 120 - 234 9e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 152 - 281 2.6e-07 WDHD1 first WD40 domain
WD40_Prp19 PF24814 159 - 299 8.6e-14 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 161 - 279 7.4e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 168 - 295 6.8e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 132
AccII CGCG 1 cut(s) 1006
AciI CCGC 5 cut(s) 515, 771, 899, 944, 1004
AclWI GGATC 3 cut(s) 358, 371, 634
AcoI YGGCCR 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 82
AfaI GTAC 1 cut(s) 865
AfiI CCNNNNNNNGG 2 cut(s) 132, 706
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 3 cut(s) 376, 694, 1014
AjnI CCWGG 2 cut(s) 131, 141
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
AluBI AGCT 5 cut(s) 96, 287, 396, 457, 986
AluI AGCT 5 cut(s) 96, 287, 396, 457, 986
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 1 cut(s) 498
AlwI GGATC 3 cut(s) 358, 371, 634
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 5 cut(s) 430, 468, 544, 593, 947
ArsI GACNNNNNNTTYG 2 cut(s) 829, 861
Asp700I GAANNNNTTC 2 cut(s) 205, 560
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 1 cut(s) 297
AsuHPI GGTGA 2 cut(s) 107, 556
AsuNHI GCTAGC 1 cut(s) 185
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 1 cut(s) 297
BamHI GGATCC 1 cut(s) 363
BanII GRGCYC 1 cut(s) 440
BbsI GAAGAC 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 233
BbvCI CCTCAGC 1 cut(s) 987
BbvI GCAGC 5 cut(s) 442, 455, 556, 580, 959
BccI CCATC 5 cut(s) 225, 242, 753, 776, 872
BceAI ACGGC 2 cut(s) 314, 528
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 2 cut(s) 133, 143
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 1 cut(s) 604
BcoDI GTCTC 1 cut(s) 498
BfaI CTAG 2 cut(s) 186, 383
BfuI GTATCC 1 cut(s) 415
BisI GCNGC 7 cut(s) 431, 469, 545, 594, 945, 948, 1004
BlsI GCNGC 7 cut(s) 432, 470, 546, 595, 946, 949, 1005
Bme1390I CCNGG 2 cut(s) 133, 143
Bme18I GGWCC 1 cut(s) 297
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 2 cut(s) 365, 883
BmrFI CCNGG 2 cut(s) 133, 143
BmsI GCATC 3 cut(s) 140, 328, 661
BmtI GCTAGC 1 cut(s) 189
BpiI GAAGAC 1 cut(s) 253
BplI GAGNNNNNCTC 2 cut(s) 707, 739
BpmI CTGGAG 1 cut(s) 288
Bpu10I CCTNAGC 2 cut(s) 92, 987
BpuEI CTTGAG 1 cut(s) 809
BsaJI CCNNGG 3 cut(s) 132, 195, 300
BsaXI ACNNNNNCTCC 4 cut(s) 631, 661, 907, 937
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 706
Bse118I RCCGGY 2 cut(s) 16, 20
Bse1I ACTGG 2 cut(s) 394, 1021
Bse3DI GCAATG 2 cut(s) 718, 837
BseBI CCWGG 2 cut(s) 133, 143
BseDI CCNNGG 3 cut(s) 132, 195, 300
BseGI GGATG 5 cut(s) 217, 250, 278, 667, 745
BseLI CCNNNNNNNGG 2 cut(s) 132, 706
BseMI GCAATG 2 cut(s) 718, 837
BseMII CTCAG 2 cut(s) 83, 978
BseNI ACTGG 2 cut(s) 394, 1021
BseX3I CGGCCG 1 cut(s) 18
BseXI GCAGC 5 cut(s) 442, 455, 556, 580, 959
BseYI CCCAGC 2 cut(s) 153, 439
BsgI GTGCAG 2 cut(s) 862, 945
Bsh1236I CGCG 1 cut(s) 1006
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 3 cut(s) 17, 21, 221
BslFI GGGAC 3 cut(s) 149, 523, 815
BslI CCNNNNNNNGG 2 cut(s) 132, 706
BsmAI GTCTC 1 cut(s) 498
BsmFI GGGAC 3 cut(s) 149, 523, 815
BsmI GAATGC 1 cut(s) 596
BsnI GGCC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 233, 440
Bsp143I GATC 3 cut(s) 363, 604, 626
BspACI CCGC 5 cut(s) 515, 771, 899, 944, 1004
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 2 cut(s) 84, 979
BspFNI CGCG 1 cut(s) 1006
BspLI GGNNCC 2 cut(s) 365, 883
BspOI GCTAGC 1 cut(s) 189
BspPI GGATC 3 cut(s) 358, 371, 634
BsrDI GCAATG 2 cut(s) 718, 837
BsrFI RCCGGY 2 cut(s) 16, 20
BsrI ACTGG 2 cut(s) 394, 1021
BssAI RCCGGY 2 cut(s) 16, 20
BssECI CCNNGG 3 cut(s) 132, 195, 300
BssMI GATC 3 cut(s) 363, 604, 626
BssT1I CCWWGG 2 cut(s) 195, 300
Bst2UI CCWGG 2 cut(s) 133, 143
Bst4CI ACNGT 5 cut(s) 170, 259, 325, 409, 492
BstC8I GCNNGC 5 cut(s) 18, 22, 187, 285, 775
BstDEI CTNAG 3 cut(s) 92, 288, 987
BstF5I GGATG 5 cut(s) 217, 250, 278, 667, 745
BstFNI CGCG 1 cut(s) 1006
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 3 cut(s) 366, 607, 629
BstMAI GTCTC 1 cut(s) 498
BstMBI GATC 3 cut(s) 363, 604, 626
BstMCI CGRYCG 1 cut(s) 21
BstNI CCWGG 2 cut(s) 133, 143
BstSCI CCNGG 2 cut(s) 131, 141
BstUI CGCG 1 cut(s) 1006
BstV1I GCAGC 5 cut(s) 442, 455, 556, 580, 959
BstV2I GAAGAC 1 cut(s) 253
BstX2I RGATCY 2 cut(s) 363, 626
BstYI RGATCY 2 cut(s) 363, 626
BstZI CGGCCG 1 cut(s) 18
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 1 cut(s) 20
BtsCI GGATG 5 cut(s) 217, 250, 278, 667, 745
BtsIMutI CAGTG 3 cut(s) 175, 387, 489
Cac8I GCNNGC 5 cut(s) 18, 22, 187, 285, 775
CfoI GCGC 1 cut(s) 445
Cfr10I RCCGGY 2 cut(s) 16, 20
Cfr13I GGNCC 1 cut(s) 297
CsiI ACCWGGT 1 cut(s) 141
Csp6I GTAC 1 cut(s) 864
CviAII CATG 3 cut(s) 13, 335, 355
CviQI GTAC 1 cut(s) 864
DdeI CTNAG 3 cut(s) 92, 288, 987
DpnI GATC 3 cut(s) 365, 606, 628
DpnII GATC 3 cut(s) 363, 604, 626
EaeI YGGCCR 1 cut(s) 18
EagI CGGCCG 1 cut(s) 18
EciI GGCGGA 1 cut(s) 530
EclXI CGGCCG 1 cut(s) 18
Eco130I CCWWGG 2 cut(s) 195, 300
Eco24I GRGCYC 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 297
Eco52I CGGCCG 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 131, 141
EcoT14I CCWWGG 2 cut(s) 195, 300
EcoT22I ATGCAT 1 cut(s) 895
EcoT38I GRGCYC 1 cut(s) 440
ErhI CCWWGG 2 cut(s) 195, 300
FaeI CATG 3 cut(s) 16, 338, 358
FaqI GGGAC 3 cut(s) 149, 523, 815
FatI CATG 3 cut(s) 12, 334, 354
FauNDI CATATG 1 cut(s) 895
FbaI TGATCA 1 cut(s) 604
Fnu4HI GCNGC 7 cut(s) 431, 469, 545, 594, 945, 948, 1004
FokI GGATG 5 cut(s) 204, 257, 285, 674, 732
FriOI GRGCYC 1 cut(s) 440
Fsp4HI GCNGC 7 cut(s) 431, 469, 545, 594, 945, 948, 1004
FspBI CTAG 2 cut(s) 186, 383
GlaI GCGC 1 cut(s) 444
GluI GCNGC 7 cut(s) 431, 469, 545, 594, 945, 948, 1004
GsaI CCCAGC 2 cut(s) 157, 443
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 1 cut(s) 20
HapII CCGG 3 cut(s) 17, 21, 221
HhaI GCGC 1 cut(s) 445
Hin1II CATG 3 cut(s) 16, 338, 358
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 4 cut(s) 37, 77, 615, 822
HpaII CCGG 3 cut(s) 17, 21, 221
HphI GGTGA 2 cut(s) 107, 556
Hpy166II GTNNAC 3 cut(s) 173, 730, 754
Hpy188I TCNGA 4 cut(s) 30, 579, 738, 781
Hpy188III TCNNGA 2 cut(s) 267, 659
Hpy8I GTNNAC 3 cut(s) 173, 730, 754
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 3 cut(s) 61, 235, 700
HpyCH4III ACNGT 5 cut(s) 170, 259, 325, 409, 492
HpyCH4IV ACGT 1 cut(s) 761
HpyF3I CTNAG 3 cut(s) 92, 288, 987
HpySE526I ACGT 1 cut(s) 761
Hsp92II CATG 3 cut(s) 16, 338, 358
HspAI GCGC 1 cut(s) 443
KroI GCCGGC 2 cut(s) 16, 20
KroNI GCCGGC 2 cut(s) 18, 22
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 3 cut(s) 363, 604, 626
LmnI GCTCC 2 cut(s) 462, 915
Lsp1109I GCAGC 5 cut(s) 442, 455, 556, 580, 959
LweI GCATC 3 cut(s) 140, 328, 661
MabI ACCWGGT 1 cut(s) 141
MaeI CTAG 2 cut(s) 186, 383
MaeII ACGT 1 cut(s) 761
MaeIII GTNAC 2 cut(s) 275, 385
MalI GATC 3 cut(s) 365, 606, 628
MboI GATC 3 cut(s) 363, 604, 626
MboII GAAGA 1 cut(s) 253
MflI RGATCY 2 cut(s) 363, 626
MhlI GDGCHC 2 cut(s) 233, 440
MluCI AATT 2 cut(s) 556, 793
MlyI GAGTC 2 cut(s) 71, 816
MmeI TCCRAC 1 cut(s) 604
Mph1103I ATGCAT 1 cut(s) 895
MroNI GCCGGC 2 cut(s) 16, 20
MroXI GAANNNNTTC 2 cut(s) 205, 560
MseI TTAA 3 cut(s) 348, 792, 870
MslI CAYNNNNRTG 1 cut(s) 660
MspA1I CMGCKG 1 cut(s) 773
MspI CCGG 3 cut(s) 17, 21, 221
MspR9I CCNGG 2 cut(s) 133, 143
Mva1269I GAATGC 1 cut(s) 596
MvaI CCWGG 2 cut(s) 133, 143
MvnI CGCG 1 cut(s) 1006
NaeI GCCGGC 2 cut(s) 18, 22
NdeI CATATG 1 cut(s) 895
NdeII GATC 3 cut(s) 363, 604, 626
NgoMIV GCCGGC 2 cut(s) 16, 20
NheI GCTAGC 1 cut(s) 185
NlaIII CATG 3 cut(s) 16, 338, 358
NlaIV GGNNCC 2 cut(s) 365, 883
NmuCI GTSAC 2 cut(s) 275, 385
NsiI ATGCAT 1 cut(s) 895
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 1 cut(s) 596
PdiI GCCGGC 2 cut(s) 18, 22
PdmI GAANNNNTTC 2 cut(s) 205, 560
PfeI GAWTC 2 cut(s) 37, 615
PflMI CCANNNNNTGG 1 cut(s) 132
PkrI GCNGC 7 cut(s) 432, 470, 546, 595, 946, 949, 1005
PleI GAGTC 2 cut(s) 71, 816
PpsI GAGTC 2 cut(s) 71, 816
Psp6I CCWGG 2 cut(s) 131, 141
PspFI CCCAGC 2 cut(s) 153, 439
PspGI CCWGG 2 cut(s) 131, 141
PspN4I GGNNCC 2 cut(s) 365, 883
PspPI GGNCC 1 cut(s) 297
PsuI RGATCY 2 cut(s) 363, 626
RsaI GTAC 1 cut(s) 865
RsaNI GTAC 1 cut(s) 864
RseI CAYNNNNRTG 1 cut(s) 660
SaqAI TTAA 3 cut(s) 348, 792, 870
SatI GCNGC 7 cut(s) 431, 469, 545, 594, 945, 948, 1004
Sau3AI GATC 3 cut(s) 363, 604, 626
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 2 cut(s) 71, 816
ScrFI CCNGG 2 cut(s) 133, 143
SduI GDGCHC 2 cut(s) 233, 440
SexAI ACCWGGT 1 cut(s) 141
SfaNI GCATC 3 cut(s) 140, 328, 661
SinI GGWCC 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 660
SmlI CTYRAG 1 cut(s) 824
SmoI CTYRAG 1 cut(s) 824
Sse9I AATT 2 cut(s) 556, 793
SsiI CCGC 5 cut(s) 515, 771, 899, 944, 1004
SspI AATATT 1 cut(s) 526
SspMI CTAG 2 cut(s) 186, 383
StyD4I CCNGG 2 cut(s) 131, 141
StyI CCWWGG 2 cut(s) 195, 300
TaaI ACNGT 5 cut(s) 170, 259, 325, 409, 492
TaiI ACGT 1 cut(s) 764
TaqI TCGA 4 cut(s) 5, 54, 86, 148
TasI AATT 2 cut(s) 556, 793
TauI GCSGC 2 cut(s) 947, 1006
TfiI GAWTC 2 cut(s) 37, 615
Tru1I TTAA 3 cut(s) 348, 792, 870
Tru9I TTAA 3 cut(s) 348, 792, 870
TscAI CASTG 3 cut(s) 175, 394, 489
TseFI GTSAC 2 cut(s) 275, 385
TseI GCWGC 5 cut(s) 430, 468, 544, 593, 947
Tsp45I GTSAC 2 cut(s) 275, 385
TspRI CASTG 3 cut(s) 175, 394, 489
Van91I CCANNNNNTGG 1 cut(s) 132
VpaK11BI GGWCC 1 cut(s) 297
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XmnI GAANNNNTTC 2 cut(s) 205, 560
XspI CTAG 2 cut(s) 186, 383
Zsp2I ATGCAT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.