FvH4_1g22470

Prolyl oligopeptidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005168
Physical Location & Seq
Reverse (-)
14394896 .. 14399019
4124 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g22470.t1

Sequence Viewer

Length: 3957 bp
ATGGATGAATGCTTCAACGACTTCAGGGCGGATATTGGTAAGGTTCAGGAGCGACTCCGTGAACGTGGCCGTCTGATTAAGGAACTTCGCTCGAAAGTTTCAAGTGTTGCAACCACTCCCGGTCCACGGTATGCTCCAGAAGACCCGACTCTTCCACGCCCCTGGAGAGGTGTGATAGATGGTTCAACAGGTTCACTGTATTACTGGAACCCTGCAACCCACCAGACCCAGTATGAGAAGCCATGTGAACAAGCTTGGATTACCCACCCAGAGGATGTCTCTTGGGACGACTCGGCTAAGGTCCTGATTTCCACGAGTGATGGTTCATGTAGTCACTCTGATTTGAGTTATCAAACTCACTCTCACGCATCTGATGCAACCAATGTTGAGATTGCGATTAGCAATGTGGTTGGGCTACCCAAACCGGAGGCTGTTGACATACATGGTTTCATTTCCGAGGAGATCAGCCTCGCCTCACCAAATCCCAACAGCGATGACAACGTCGTTGCCGGTGGCACCATTACTGACGTCGCCCTCGGCAGCGCTCCACTGCTCGACCATGAACTTGTTAGCCTCAACGCTTCGGCCGCCATGGTATCAACTGTTTCACACCATCTCAGTACCGTTATCTCGGAGTCAACTTTGTCCTCCACAAATGGAAGCGTTCTCGGCAGCGCTACTACACCCAAGAAGTTCAAAACGGTTCCAGTTTACCACCACCTTAAGACACGGTCGAATATCAAGATGAAGCGACAACCACTGTCTAAGCCCGCTCGCCGTAGTCGAACTCGAATAGGGGGCGATTCAGAGACGACCAAGTACGGCCCTCCTTCTTTGATTATGTCCACCAAAGCTGATGATTTGGATGTGCATGATGACCCGTCAATGGAGCTTGCATTTTACCCACAGACTCTAACTGGAACGACGATGGCCTTTGAGAAGCTTCAATCAATGCGGCTTTCATTCAGACCTGAAGATTACTATGCAGAAATGGTGAAGTCGGCGTCCGAATTGGAGAACATGAATAGCCAACTTTCGGTGCAGATGACTACCAATCAGCGTCCTGCCTCATTGTCTCAGGTATTCACCAAGTGGAATTTAGTTTTTCAAGCTTTTAGAGGGCACTCTGTGAGTAATTTGTTCGGGCTCTATCATCATTGTTTGAATGGTAAAGTTGGACAAGAAAAATGGCCTCCTGATGAGTGGATCGAGAAAATGAAATGGGATGTCAACAATTTGTTTGACTGGTTGAATAGTACTATATCAGAGTTTGGCACTGTGGCTACCTTAAATGCATGGTTGCATGATATGTTTATGTGTCAACCTCTTGAGTACAGACTAGAGAACATGAGTTTATTTCTTGTTATCATGATGAAGAGCCAGTCAACAGATGTTGGGAACTCAATTGTGCAGTTGTTGGGAGGTATACTTGAGCCTGCAATGTTTTGGGTTATCTATACCACTCATGGTCAGATGCATAAGACCAAGTTTTTTTCTTCTAAACAAGGGTTGTTGGACATCGATTACCCATACCAGACAACAATTAACAAACTTCCTGCTAGCATGAAAGGCTGGCCACATGAAACTTTTAGGAATCCATGGCGCAAGAGGACGACTAATGTGCGCTATTTCATTGAATTCCTAGAAATTGGCTGCTATTACGAGTCAGCGATCATGTTAACTTCTCCGGCTCGGGATATTGTGCTATCGATTTCAGCTAAGGATTTGGTGGCAGATCTCAGTAAAACACCGGGTTGCATGTTGTTTATTCGGACCTTGTTGCACATGTTCATGGATGAAAGTCATGCTTCTGGTGAACCTTCAGAATTCTATCCGGCCACAGTTGATATTGCCCTTGCTATGATAATTGTCAGTTTTGTGTTTCTATTTGCAGTCTTGCATGTTTGTTACAAGTTTCATGGCTGTCATAGCACAGAAAAAGACTTGAGGTCCAGAAAGAATGGGCGCGATGATTCTATTTTCCAGAAAGGCAGTGGCAAGCTTCTCTCAATATTGGTGGCAGGAGATGTTGCTTCACGAGAGTTGGATCTTTTTCATGTCACAACTTATGACTTTCCAAAAGGCATACATGATACTGTTCACACATTTGGCCGTGCTGGTATGGTAGTGAAGTTAATTAAGGAATCCATGAAGGAGGTTCCTGATTGGCTTAATGATGATGCTACACAATTATTGTCTTATGGTGGAGAACACAGCCAATGGTATGGAAGTAATGACTTTGGAAATTTTGCATTGATTCCTCCAACACACTTTCCACTACCTTTGACAAATGATGCTCTTTTCCTCATCTTATTGATGCGACTTATATTCCTTCAGTTAATTACATTTGTGATTTCCAGACCAATGCATTTGACATCCCCAGGTATCAATTTTGGAATTCCGAAAATTATTAAGCACTGCGTCATGGCAGATTTTAATTGGAGATTCCAGGAGAAAATAATTTTTCAGAACAATAGAAGTATAAGCACTGGTTATGCTAGAGAATATCGCGCCAGGCTGCTGAGGAAATGGGGAATTGTGTATGTAACTGCTTGTTGTGGCTGTGCTAGTTATTTGGTGAAATCTGGTAATGTAGATGGGAAACGACTGTATATTACTGTGGGCTCTGCAAGTCCGTACACATCCTCGGCTATTCTAGTAATCAGAGAACTCTTCAGGGCTAGAGCTCCCTTGTCTGGTGTCGCAAACTTTAACATGTTCAGGGCAGAAACATGCAGCCTTGAGTCTCATTATATGGATAATCATGTTGATGATGACAATAATTTCTTTGTTTTGTCACCAATCAACCTTGGTGTTCAATGTTCTTGCCCCATGATATTATTCCAAGGCCAGGAAGGCAAGATTGTACCCGAATTCTTCTTTTTGCGCGCATTCAGAGTAGCTGACCACTGTCCCCTCGGAATTTTTGTACTTAATTCTATCAATCAGTTGGTGTCTTTGAAGTTTATGAATCAGGGCCTTGAAAGAAAGGAAGCTTTTATGCTTATCTTGTTGTCTACACTTATGCACTCCCAACTGATTGGAATTGGGATTTCAGTCTATCTACTACCTGGTGTCAATGGAGAAGCCTTGAGGGTATTTTCTAATGTGCAAATTCAGTATTGTTGGCAGTCAATTCCTAATTCTCATGTTAGTGTTCATCCATTAATACATAATTGTCAAGTTACTACTGCTGATGGCATTGCAATTGGTGCAACCGTGATAGATATTACATCAGATGCCACTCATGGGGACTCCAGATCTTTTACTTACCAAGTTATTGAGGGGCCTGCAGAACAGATGCCTTTGCTTCTCAGAATTTGGACTGCATGTGCATCAGTAGAGACCAAATTTTTTCACCATTTTCAGTTGTTTTTAACATATGTTAGTGGGGTGAAAGCAAGCCAAGGTGCAGTGTTTGTATTTGTCTGTGGGGATCAAGGTGCACATGCTTTGATAACATACTCAGAGGTCATAAATGGATATATCATCTCTACTGTTGATTTGTTGTATATCATTAACCGAGCTGACACGGTAGGAATGCACGACTTCTCTAGACCTGGGCAATTCGGCAACAGCAGAGTTTGGGTCAACGACACTACCTGTTTTTCAAGGCAGATGGGAAATTTGGAAGCACACTTTCTTTCTGAGAAGAAAGTAATCCACCAGTCAGTAACATCTGATCTTAAGAAGGGGACTACCGAAACTGGGAGGCAACAAACTGTGTCTTGTGTGACAGATGCTGATCTCCTACTCAGGTTTTTACTTCTGACTCTTACAGAAGAGAAGAGTAAGCTGTTGATACTGGTATACTATTACCCATGCCAACTTACCAGTTATTGCCTGTTGTTTTTTCGTCTTGCTATGGGAAAATGGATGCATGAAGTTGACTGGATGATCAACACAGTGCAATGGAATGGTTTCTACTGCAGCAATCTTGGCACAGTCAAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

1319

Amino Acids

148.06

Weight (kDa)

6.5

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WW PF00397 51 - 81 3.2e-06 WW domain
Ebp2 PF05890 280 - 353 1.5e-12 Eukaryotic rRNA processing protein EBP2
Peptidase_S9 PF00326 820 - 956 3.6e-09 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 531
AccB1I GGYRCC 1 cut(s) 515
AccBSI CCGCTC 1 cut(s) 773
AccI GTMKAC 3 cut(s) 1426, 3017, 3810
AccII CGCG 3 cut(s) 1968, 2511, 2889
AciI CCGC 4 cut(s) 29, 588, 771, 955
AclWI GGATC 3 cut(s) 1214, 2055, 3444
AcoI YGGCCR 5 cut(s) 67, 585, 1574, 1836, 2110
AcuI CTGAAG 5 cut(s) 7, 993, 1806, 2318, 2659
AcyI GRCGYC 2 cut(s) 528, 1004
AdeI CACNNNGTG 2 cut(s) 1092, 1129
AfaI GTAC 7 cut(s) 622, 821, 1258, 1334, 2639, 2868, 2931
AfeI AGCGCT 2 cut(s) 544, 676
AfiI CCNNNNNNNGG 9 cut(s) 126, 167, 271, 886, 1036, 2696, 2851, 3249, 3708
AflII CTTAAG 2 cut(s) 722, 3686
AflIII ACRYGT 2 cut(s) 1785, 2715
AhdI GACNNNNNGTC 1 cut(s) 1948
AjnI CCWGG 7 cut(s) 161, 2380, 2448, 2513, 2850, 3070, 3559
Alw21I GWGCWC 2 cut(s) 2689, 3448
Alw26I GTCTC 5 cut(s) 283, 803, 1080, 2751, 3338
Alw44I GTGCAC 1 cut(s) 3444
AlwI GGATC 3 cut(s) 1214, 2055, 3444
AlwNI CAGNNNCTG 1 cut(s) 3743
Ama87I CYCGRG 1 cut(s) 1692
Aor51HI AGCGCT 2 cut(s) 544, 676
ApaLI GTGCAC 1 cut(s) 3444
ApeKI GCWGC 6 cut(s) 540, 672, 1653, 2518, 2736, 3930
AseI ATTAAT 1 cut(s) 3167
Asp700I GAANNNNTTC 1 cut(s) 3920
AspLEI GCGC 8 cut(s) 545, 677, 1605, 1626, 1968, 2513, 2889, 2891
AspS9I GGNCC 7 cut(s) 122, 301, 824, 1773, 1950, 2977, 3287
AsuC2I CCSGG 2 cut(s) 120, 1752
AsuHPI GGTGA 8 cut(s) 468, 1006, 1078, 1826, 2590, 2790, 3350, 3406
AsuNHI GCTAGC 1 cut(s) 1559
AvaI CYCGRG 1 cut(s) 1692
AvaII GGWCC 4 cut(s) 122, 301, 1773, 1950
BaeGI GKGCMC 2 cut(s) 1125, 3448
BalI TGGCCA 1 cut(s) 1576
BanI GGYRCC 1 cut(s) 515
BanII GRGCYC 3 cut(s) 1149, 2627, 2689
BauI CACGAG 2 cut(s) 313, 2037
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 2 cut(s) 2689, 3448
BbvCI CCTCAGC 1 cut(s) 2522
BbvI GCAGC 6 cut(s) 552, 684, 1640, 2505, 2748, 3942
BccI CCATC 7 cut(s) 173, 314, 621, 922, 2591, 3191, 3613
BceAI ACGGC 4 cut(s) 54, 762, 838, 2097
BcgI CGANNNNNNTGC 2 cut(s) 1603, 1637
BciT130I CCWGG 7 cut(s) 163, 2382, 2450, 2515, 2852, 3072, 3561
BclI TGATCA 1 cut(s) 3897
BcnI CCSGG 2 cut(s) 120, 1752
BcoDI GTCTC 5 cut(s) 283, 803, 1080, 2751, 3338
BfaI CTAG 8 cut(s) 1340, 1560, 1643, 2499, 2568, 2657, 2682, 3555
BfmI CTRYAG 2 cut(s) 3291, 3928
BfoI RGCGCY 2 cut(s) 546, 678
BfrI CTTAAG 2 cut(s) 722, 3686
BglI GCCNNNNNGGC 1 cut(s) 2856
BglII AGATCT 2 cut(s) 1735, 3260
BisI GCNGC 8 cut(s) 541, 588, 673, 956, 1654, 2519, 2737, 3931
BlsI GCNGC 8 cut(s) 542, 589, 674, 957, 1655, 2520, 2738, 3932
BmcAI AGTACT 1 cut(s) 1258
Bme1390I CCNGG 9 cut(s) 120, 163, 1752, 2382, 2450, 2515, 2852, 3072, 3561
Bme18I GGWCC 4 cut(s) 122, 301, 1773, 1950
BmeRI GACNNNNNGTC 1 cut(s) 1948
BmeT110I CYCGRG 1 cut(s) 1692
BmgT120I GGNCC 7 cut(s) 122, 301, 824, 1773, 1950, 2977, 3287
BmiI GGNNCC 5 cut(s) 209, 517, 705, 2160, 3288
BmrFI CCNGG 9 cut(s) 120, 163, 1752, 2382, 2450, 2515, 2852, 3072, 3561
BmrI ACTGGG 2 cut(s) 223, 3717
BmtI GCTAGC 1 cut(s) 1563
BmuI ACTGGG 2 cut(s) 223, 3717
BoxI GACNNNNGTC 1 cut(s) 2910
BpiI GAAGAC 1 cut(s) 147
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpmI CTGGAG 3 cut(s) 120, 184, 3241
Bpu10I CCTNAGC 3 cut(s) 297, 1719, 2522
BpuEI CTTGAG 5 cut(s) 1349, 1451, 1966, 2762, 3112
BpuMI CCSGG 2 cut(s) 120, 1752
Bsa29I ATCGAT 2 cut(s) 1521, 1709
BsaBI GATNNNNATC 1 cut(s) 3744
BsaHI GRCGYC 2 cut(s) 528, 1004
BsaI GGTCTC 1 cut(s) 3338
BsaWI WCCGGW 1 cut(s) 424
Bsc4I CCNNNNNNNGG 9 cut(s) 126, 167, 271, 886, 1036, 2696, 2851, 3249, 3708
Bse118I RCCGGY 1 cut(s) 509
Bse3DI GCAATG 4 cut(s) 409, 1446, 3201, 3917
Bse8I GATNNNNATC 1 cut(s) 3744
BseBI CCWGG 7 cut(s) 163, 2382, 2450, 2515, 2852, 3072, 3561
BseCI ATCGAT 2 cut(s) 1521, 1709
BseJI GATNNNNATC 1 cut(s) 3744
BseLI CCNNNNNNNGG 9 cut(s) 126, 167, 271, 886, 1036, 2696, 2851, 3249, 3708
BseMI GCAATG 4 cut(s) 409, 1446, 3201, 3917
BseMII CTCAG 8 cut(s) 631, 1091, 1753, 2513, 3328, 3480, 3639, 3769
BsePI GCGCGC 1 cut(s) 2887
BseRI GAGGAG 1 cut(s) 473
BseSI GKGCMC 2 cut(s) 1125, 3448
BseX3I CGGCCG 1 cut(s) 585
BseXI GCAGC 6 cut(s) 552, 684, 1640, 2505, 2748, 3942
BsgI GTGCAG 3 cut(s) 1061, 1430, 3432
Bsh1236I CGCG 3 cut(s) 1968, 2511, 2889
Bsh1285I CGRYCG 2 cut(s) 588, 734
BshNI GGYRCC 1 cut(s) 515
BshVI ATCGAT 2 cut(s) 1521, 1709
BsiEI CGRYCG 2 cut(s) 588, 734
BsiHKAI GWGCWC 2 cut(s) 2689, 3448
BsiHKCI CYCGRG 1 cut(s) 1692
BsiSI CCGG 6 cut(s) 120, 425, 510, 1688, 1751, 1835
BslFI GGGAC 4 cut(s) 299, 2898, 3266, 3709
BslI CCNNNNNNNGG 9 cut(s) 126, 167, 271, 886, 1036, 2696, 2851, 3249, 3708
BsmAI GTCTC 5 cut(s) 283, 803, 1080, 2751, 3338
BsmBI CGTCTC 1 cut(s) 803
BsmFI GGGAC 4 cut(s) 299, 2898, 3266, 3709
BsmI GAATGC 3 cut(s) 14, 2891, 3546
Bso31I GGTCTC 1 cut(s) 3338
BsoBI CYCGRG 1 cut(s) 1692
Bsp1286I GDGCHC 5 cut(s) 1125, 1149, 2627, 2689, 3448
Bsp19I CCATGG 2 cut(s) 591, 1598
BspACI CCGC 4 cut(s) 29, 588, 771, 955
BspCNI CTCAG 8 cut(s) 630, 1090, 1752, 2514, 3327, 3479, 3640, 3768
BspDI ATCGAT 2 cut(s) 1521, 1709
BspFNI CGCG 3 cut(s) 1968, 2511, 2889
BspHI TCATGA 1 cut(s) 1368
BspLI GGNNCC 5 cut(s) 209, 517, 705, 2160, 3288
BspMAI CTGCAG 2 cut(s) 3295, 3932
BspOI GCTAGC 1 cut(s) 1563
BspPI GGATC 3 cut(s) 1214, 2055, 3444
BspQI GCTCTTC 1 cut(s) 1370
BspT107I GGYRCC 1 cut(s) 515
BspTI CTTAAG 2 cut(s) 722, 3686
BspTNI GGTCTC 1 cut(s) 3338
BsrBI CCGCTC 1 cut(s) 773
BsrDI GCAATG 4 cut(s) 409, 1446, 3201, 3917
BsrFI RCCGGY 1 cut(s) 509
BssAI RCCGGY 1 cut(s) 509
BssHII GCGCGC 1 cut(s) 2887
BssNAI GTATAC 2 cut(s) 1427, 3811
BssNI GRCGYC 2 cut(s) 528, 1004
BssSI CACGAG 2 cut(s) 313, 2037
BssT1I CCWWGG 5 cut(s) 591, 1598, 2809, 2845, 3406
Bst1107I GTATAC 2 cut(s) 1427, 3811
Bst2BI CACGAG 2 cut(s) 313, 2037
Bst2UI CCWGG 7 cut(s) 163, 2382, 2450, 2515, 2852, 3072, 3561
Bst6I CTCTTC 5 cut(s) 156, 1370, 2678, 3777, 3782
BstACI GRCGYC 2 cut(s) 528, 1004
BstAFI CTTAAG 2 cut(s) 722, 3686
BstAPI GCANNNNNTGC 2 cut(s) 374, 3212
BstDSI CCRYGG 3 cut(s) 125, 591, 1598
BstFNI CGCG 3 cut(s) 1968, 2511, 2889
BstH2I RGCGCY 2 cut(s) 546, 678
BstHHI GCGC 8 cut(s) 545, 677, 1605, 1626, 1968, 2513, 2889, 2891
BstMAI GTCTC 5 cut(s) 283, 803, 1080, 2751, 3338
BstMCI CGRYCG 2 cut(s) 588, 734
BstMWI GCNNNNNNNGC 8 cut(s) 374, 587, 669, 1569, 1929, 2856, 3212, 3939
BstNI CCWGG 7 cut(s) 163, 2382, 2450, 2515, 2852, 3072, 3561
BstNSI RCATGY 7 cut(s) 1762, 1789, 1904, 2719, 2736, 3333, 3452
BstPAI GACNNNNGTC 1 cut(s) 2910
BstSCI CCNGG 9 cut(s) 118, 161, 1750, 2380, 2448, 2513, 2850, 3070, 3559
BstSFI CTRYAG 2 cut(s) 3291, 3928
BstSLI GKGCMC 2 cut(s) 1125, 3448
BstUI CGCG 3 cut(s) 1968, 2511, 2889
BstV1I GCAGC 6 cut(s) 552, 684, 1640, 2505, 2748, 3942
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 3 cut(s) 1735, 2047, 3260
BstXI CCANNNNNNTGG 3 cut(s) 162, 2225, 3041
BstYI RGATCY 3 cut(s) 1735, 2047, 3260
BstZ17I GTATAC 2 cut(s) 1427, 3811
BstZI CGGCCG 1 cut(s) 585
Bsu15I ATCGAT 2 cut(s) 1521, 1709
BsuTUI ATCGAT 2 cut(s) 1521, 1709
BtgI CCRYGG 3 cut(s) 125, 591, 1598
BtgZI GCGATG 2 cut(s) 507, 1983
BtsI GCAGTG 4 cut(s) 548, 1999, 2416, 3420
CaiI CAGNNNCTG 1 cut(s) 3743
CciI TCATGA 1 cut(s) 1368
CfoI GCGC 8 cut(s) 545, 677, 1605, 1626, 1968, 2513, 2889, 2891
Cfr10I RCCGGY 1 cut(s) 509
Cfr13I GGNCC 7 cut(s) 122, 301, 824, 1773, 1950, 2977, 3287
ClaI ATCGAT 2 cut(s) 1521, 1709
CseI GACGC 3 cut(s) 993, 1049, 2410
CsiI ACCWGGT 1 cut(s) 3070
Csp6I GTAC 7 cut(s) 621, 820, 1257, 1333, 2638, 2867, 2930
CspCI CAANNNNNGTGG 4 cut(s) 1998, 2033, 2265, 2300
CviQI GTAC 7 cut(s) 621, 820, 1257, 1333, 2638, 2867, 2930
DraIII CACNNNGTG 2 cut(s) 1092, 1129
DriI GACNNNNNGTC 1 cut(s) 1948
EaeI YGGCCR 5 cut(s) 67, 585, 1574, 1836, 2110
EagI CGGCCG 1 cut(s) 585
Eam1104I CTCTTC 5 cut(s) 156, 1370, 2678, 3777, 3782
Eam1105I GACNNNNNGTC 1 cut(s) 1948
EarI CTCTTC 5 cut(s) 156, 1370, 2678, 3777, 3782
EciI GGCGGA 1 cut(s) 44
Ecl136II GAGCTC 1 cut(s) 2687
EclXI CGGCCG 1 cut(s) 585
Eco130I CCWWGG 5 cut(s) 591, 1598, 2809, 2845, 3406
Eco147I AGGCCT 1 cut(s) 3953
Eco24I GRGCYC 3 cut(s) 1149, 2627, 2689
Eco31I GGTCTC 1 cut(s) 3338
Eco47I GGWCC 4 cut(s) 122, 301, 1773, 1950
Eco47III AGCGCT 2 cut(s) 544, 676
Eco52I CGGCCG 1 cut(s) 585
Eco53kI GAGCTC 1 cut(s) 2687
Eco57I CTGAAG 5 cut(s) 7, 993, 1806, 2318, 2659
Eco88I CYCGRG 1 cut(s) 1692
EcoICRI GAGCTC 1 cut(s) 2687
EcoO109I RGGNCCY 3 cut(s) 301, 2977, 3287
EcoRI GAATTC 4 cut(s) 1637, 1826, 2397, 2873
EcoRII CCWGG 7 cut(s) 161, 2380, 2448, 2513, 2850, 3070, 3559
EcoT14I CCWWGG 5 cut(s) 591, 1598, 2809, 2845, 3406
EcoT22I ATGCAT 4 cut(s) 1297, 1479, 2370, 3882
EcoT38I GRGCYC 3 cut(s) 1149, 2627, 2689
ErhI CCWWGG 5 cut(s) 591, 1598, 2809, 2845, 3406
Esp3I CGTCTC 1 cut(s) 803
FalI AAGNNNNNCTT 2 cut(s) 1792, 1824
FaqI GGGAC 4 cut(s) 299, 2898, 3266, 3709
FauI CCCGC 1 cut(s) 778
FauNDI CATATG 1 cut(s) 3382
FbaI TGATCA 1 cut(s) 3897
FblI GTMKAC 3 cut(s) 1426, 3017, 3810
Fnu4HI GCNGC 8 cut(s) 541, 588, 673, 956, 1654, 2519, 2737, 3931
FriOI GRGCYC 3 cut(s) 1149, 2627, 2689
Fsp4HI GCNGC 8 cut(s) 541, 588, 673, 956, 1654, 2519, 2737, 3931
FspBI CTAG 8 cut(s) 1340, 1560, 1643, 2499, 2568, 2657, 2682, 3555
GlaI GCGC 8 cut(s) 544, 676, 1604, 1625, 1967, 2512, 2888, 2890
GluI GCNGC 8 cut(s) 541, 588, 673, 956, 1654, 2519, 2737, 3931
GsuI CTGGAG 3 cut(s) 120, 184, 3241
HaeII RGCGCY 2 cut(s) 546, 678
HapII CCGG 6 cut(s) 120, 425, 510, 1688, 1751, 1835
HgaI GACGC 3 cut(s) 993, 1049, 2410
HhaI GCGC 8 cut(s) 545, 677, 1605, 1626, 1968, 2513, 2889, 2891
Hin1I GRCGYC 2 cut(s) 528, 1004
Hin6I GCGC 8 cut(s) 543, 675, 1603, 1624, 1966, 2511, 2887, 2889
HinP1I GCGC 8 cut(s) 543, 675, 1603, 1624, 1966, 2511, 2887, 2889
HincII GTYRAC 8 cut(s) 436, 639, 1231, 1322, 1386, 1680, 3592, 3889
HindII GTYRAC 8 cut(s) 436, 639, 1231, 1322, 1386, 1680, 3592, 3889
HindIII AAGCTT 5 cut(s) 252, 941, 1110, 2000, 2994
HpaI GTTAAC 1 cut(s) 1680
HpaII CCGG 6 cut(s) 120, 425, 510, 1688, 1751, 1835
HphI GGTGA 8 cut(s) 468, 1006, 1078, 1826, 2590, 2790, 3350, 3406
Hpy99I CGWCG 3 cut(s) 506, 533, 928
HpyAV CCTTC 6 cut(s) 840, 1830, 2146, 2342, 2849, 3685
HpyCH4IV ACGT 3 cut(s) 64, 501, 528
HpyF10VI GCNNNNNNNGC 8 cut(s) 374, 587, 669, 1569, 1929, 2856, 3212, 3939
HpySE526I ACGT 3 cut(s) 64, 501, 528
Hsp92I GRCGYC 2 cut(s) 528, 1004
HspAI GCGC 8 cut(s) 543, 675, 1603, 1624, 1966, 2511, 2887, 2889
Ksp22I TGATCA 1 cut(s) 3897
KspAI GTTAAC 1 cut(s) 1680
LguI GCTCTTC 1 cut(s) 1370
LmnI GCTCC 5 cut(s) 49, 139, 550, 889, 2692
Lsp1109I GCAGC 6 cut(s) 552, 684, 1640, 2505, 2748, 3942
MabI ACCWGGT 1 cut(s) 3070
MaeI CTAG 8 cut(s) 1340, 1560, 1643, 2499, 2568, 2657, 2682, 3555
MaeII ACGT 3 cut(s) 64, 501, 528
MaeIII GTNAC 8 cut(s) 332, 1907, 2059, 2545, 2796, 3184, 3673, 3733
MbiI CCGCTC 1 cut(s) 773
MfeI CAATTG 2 cut(s) 1404, 3207
MflI RGATCY 3 cut(s) 1735, 2047, 3260
MhlI GDGCHC 5 cut(s) 1125, 1149, 2627, 2689, 3448
MlsI TGGCCA 1 cut(s) 1576
MluNI TGGCCA 1 cut(s) 1576
MlyI GAGTC 9 cut(s) 48, 142, 284, 644, 904, 1673, 2753, 3248, 3766
MmeI TCCRAC 4 cut(s) 1156, 1494, 2025, 2288
Mox20I TGGCCA 1 cut(s) 1576
Mph1103I ATGCAT 4 cut(s) 1297, 1479, 2370, 3882
MroXI GAANNNNTTC 1 cut(s) 3920
MscI TGGCCA 1 cut(s) 1576
MslI CAYNNNNRTG 3 cut(s) 1790, 2769, 3153
Msp20I TGGCCA 1 cut(s) 1576
MspCI CTTAAG 2 cut(s) 722, 3686
MspI CCGG 6 cut(s) 120, 425, 510, 1688, 1751, 1835
MspR9I CCNGG 9 cut(s) 120, 163, 1752, 2382, 2450, 2515, 2852, 3072, 3561
MunI CAATTG 2 cut(s) 1404, 3207
Mva1269I GAATGC 3 cut(s) 14, 2891, 3546
MvaI CCWGG 7 cut(s) 163, 2382, 2450, 2515, 2852, 3072, 3561
MvnI CGCG 3 cut(s) 1968, 2511, 2889
MwoI GCNNNNNNNGC 8 cut(s) 374, 587, 669, 1569, 1929, 2856, 3212, 3939
NciI CCSGG 2 cut(s) 120, 1752
NcoI CCATGG 2 cut(s) 591, 1598
NdeI CATATG 1 cut(s) 3382
NheI GCTAGC 1 cut(s) 1559
NlaIV GGNNCC 5 cut(s) 209, 517, 705, 2160, 3288
NmeAIII GCCGAG 4 cut(s) 272, 516, 648, 2627
NmuCI GTSAC 4 cut(s) 332, 2059, 2796, 3733
NsiI ATGCAT 4 cut(s) 1297, 1479, 2370, 3882
NspI RCATGY 7 cut(s) 1762, 1789, 1904, 2719, 2736, 3333, 3452
PacI TTAATTAA 1 cut(s) 2139
PagI TCATGA 1 cut(s) 1368
PauI GCGCGC 1 cut(s) 2887
PceI AGGCCT 1 cut(s) 3953
PciI ACATGT 2 cut(s) 1785, 2715
PciSI GCTCTTC 1 cut(s) 1370
PcsI WCGNNNNNNNCGW 1 cut(s) 781
PctI GAATGC 3 cut(s) 14, 2891, 3546
PdmI GAANNNNTTC 1 cut(s) 3920
PfeI GAWTC 7 cut(s) 803, 1594, 1973, 2144, 2257, 2445, 2971
PflFI GACNNNGTC 2 cut(s) 500, 730
PfoI TCCNGGA 1 cut(s) 2448
PkrI GCNGC 8 cut(s) 542, 589, 674, 957, 1655, 2520, 2738, 3932
PleI GAGTC 9 cut(s) 48, 142, 284, 643, 904, 1672, 2752, 3248, 3766
PpsI GAGTC 9 cut(s) 48, 142, 284, 643, 904, 1672, 2752, 3248, 3766
PpuMI RGGWCCY 1 cut(s) 301
PscI ACATGT 2 cut(s) 1785, 2715
PshAI GACNNNNGTC 1 cut(s) 2910
PshBI ATTAAT 1 cut(s) 3167
Psp124BI GAGCTC 1 cut(s) 2689
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 7 cut(s) 161, 2380, 2448, 2513, 2850, 3070, 3559
PspGI CCWGG 7 cut(s) 161, 2380, 2448, 2513, 2850, 3070, 3559
PspN4I GGNNCC 5 cut(s) 209, 517, 705, 2160, 3288
PspPI GGNCC 7 cut(s) 122, 301, 824, 1773, 1950, 2977, 3287
PspPPI RGGWCCY 1 cut(s) 301
PstI CTGCAG 2 cut(s) 3295, 3932
PstNI CAGNNNCTG 1 cut(s) 3743
PsuI RGATCY 3 cut(s) 1735, 2047, 3260
PsyI GACNNNGTC 2 cut(s) 500, 730
PteI GCGCGC 1 cut(s) 2887
RsaI GTAC 7 cut(s) 622, 821, 1258, 1334, 2639, 2868, 2931
RsaNI GTAC 7 cut(s) 621, 820, 1257, 1333, 2638, 2867, 2930
RseI CAYNNNNRTG 3 cut(s) 1790, 2769, 3153
SacI GAGCTC 1 cut(s) 2689
SapI GCTCTTC 1 cut(s) 1370
SatI GCNGC 8 cut(s) 541, 588, 673, 956, 1654, 2519, 2737, 3931
Sau96I GGNCC 7 cut(s) 122, 301, 824, 1773, 1950, 2977, 3287
ScaI AGTACT 1 cut(s) 1258
SchI GAGTC 9 cut(s) 48, 142, 284, 644, 904, 1673, 2753, 3248, 3766
ScrFI CCNGG 9 cut(s) 120, 163, 1752, 2382, 2450, 2515, 2852, 3072, 3561
SduI GDGCHC 5 cut(s) 1125, 1149, 2627, 2689, 3448
SexAI ACCWGGT 1 cut(s) 3070
SfcI CTRYAG 2 cut(s) 3291, 3928
SinI GGWCC 4 cut(s) 122, 301, 1773, 1950
SmiMI CAYNNNNRTG 3 cut(s) 1790, 2769, 3153
SmlI CTYRAG 7 cut(s) 722, 1328, 1430, 1945, 2741, 3091, 3686
SmoI CTYRAG 7 cut(s) 722, 1328, 1430, 1945, 2741, 3091, 3686
SseBI AGGCCT 1 cut(s) 3953
SsiI CCGC 4 cut(s) 29, 588, 771, 955
SspI AATATT 1 cut(s) 2013
SspMI CTAG 8 cut(s) 1340, 1560, 1643, 2499, 2568, 2657, 2682, 3555
SstI GAGCTC 1 cut(s) 2689
StuI AGGCCT 1 cut(s) 3953
StyD4I CCNGG 9 cut(s) 118, 161, 1750, 2380, 2448, 2513, 2850, 3070, 3559
StyI CCWWGG 5 cut(s) 591, 1598, 2809, 2845, 3406
TaiI ACGT 3 cut(s) 67, 504, 531
TaqI TCGA 8 cut(s) 92, 555, 734, 784, 790, 1209, 1521, 1709
TatI WGTACW 3 cut(s) 1256, 1332, 2929
TauI GCSGC 2 cut(s) 590, 958
TfiI GAWTC 7 cut(s) 803, 1594, 1973, 2144, 2257, 2445, 2971
TseFI GTSAC 4 cut(s) 332, 2059, 2796, 3733
TseI GCWGC 6 cut(s) 540, 672, 1653, 2518, 2736, 3930
Tsp45I GTSAC 4 cut(s) 332, 2059, 2796, 3733
TspGWI ACGGA 2 cut(s) 47, 2625
Tth111I GACNNNGTC 2 cut(s) 500, 730
Vha464I CTTAAG 2 cut(s) 722, 3686
VneI GTGCAC 1 cut(s) 3444
VpaK11BI GGWCC 4 cut(s) 122, 301, 1773, 1950
VspI ATTAAT 1 cut(s) 3167
XbaI TCTAGA 1 cut(s) 3554
XceI RCATGY 7 cut(s) 1762, 1789, 1904, 2719, 2736, 3333, 3452
XcmI CCANNNNNNNNNTGG 1 cut(s) 1991
XmiI GTMKAC 3 cut(s) 1426, 3017, 3810
XmnI GAANNNNTTC 1 cut(s) 3920
XspI CTAG 8 cut(s) 1340, 1560, 1643, 2499, 2568, 2657, 2682, 3555
ZraI GACGTC 1 cut(s) 529
ZrmI AGTACT 1 cut(s) 1258
Zsp2I ATGCAT 4 cut(s) 1297, 1479, 2370, 3882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.