RchiOBHm_Chr6g0274801

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005168
Physical Location & Seq
Reverse (-)
36374728 .. 36379639
4912 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24654

Sequence Viewer

Length: 3693 bp
ATGTGTCATGTGTTGTGCACGTGTGCAGTGGGTTATATAAACCAGAACTCTGTACTCCTTTCATTATCGATCAATATATCAGAGTTTTCCTTTTGCATTTTTTTCTGCATTTCGTTTCTTCGTTGGGGTCGTACCCTAACAACTGGTATCAGAGCCCTCATCATGGGGCCGAGGAGCGCTGAGACTCCACCTCCATACTCGTCAGCACCGCTGCGTCCACCTGAATTAGATGAAGTGAGGAGGAAGAGGATCCAAATTGCAAGAGAACAGGCCGCTGAACAAGCAGCTGCGTGGCGCCGTGAGTTTGAGAAAACTATTGCGTCGTTCCGGTCTGAGTTACTAAATGAGCTGAGGCAATTTCGATCGGAGCTCACAGCTCAGGTGATGGAGCCGTCGAGGCCGACCTCGGCAGGACCAGTCAGATATCTGCCAGTGACTCAGCCTGTCTCCTCAGAGCTGCATTTTGGGACCATTGATTCTTACAAACCAACACCACCACCGGCCACAGCGGGTCTCTACCTGCAACAACCACCTCCGCCGCCACCTAGACCATCAGTCACCGTCCACGCGTTGGCTGAGTCACCATCCCCAGCCCACATGAGGTACACCAACCGCTCAGTCTCAATTGGTGATTTCAATCACAACATGAACCAGCAACATTTTTCCTTGAATGATGATTATGGTGCTTTGAGTCTTGTCTCTGTTGCTAAAGTGACTGATATTGATGAGAAATTAGATATGGGAGAGGGAGAGAATTCTGATAAGATGGTTAAGGTGAATAGTGGGGTTGGTAGAGATTTCAATGAGTTTAAGAGTGGCAGTGCTAAGCAGGACGAGGGTGGTTCTATTGAAGATAAGGAGCAGCTGAGCAATGGAATGTTAGAATGTCATGCCCTCCAACTGTTTGATAAAATGCCAAAGTCAGAAGGGTGGTGTAATGCAGGAAGGACCATTATTGCCCCTATGAGTATTCGAGACAAAGGTGGTGGACTCACTTCTCATCGTGAAGTTGTTGATGCTTCTGATGAGATGACAATATGTTCTGTCAGTGCTACGCAGGACGCTGGTGGTTTCATTGAAGATAAGGGGCAGGTAGACTTCACAACTCCTTTAATATCTAAATCTCTGAATACAGAAAATGATGAAATTGTGAATGCCAAAGTTGAAGTAGTGGAAACCCTTTGTGAACATTCTGATGAGAAATTAGATATTGGGGAGAAAGAGAGTTTTGATAAGAGGACAGAGAGTTCAAAGGGAAATTTGGATTTGGATGGGCTTTGGGATGATAGTGGTGGATTGTCCACCATGAAGACTAAATTTAAGAGTTTCTGCAAGCCTGCTTTGGTGCTTGGAGACTTTAAGCTGATTCATGTAGAAGACCACATGCTTGGAGATCATGAGCAAAGTGCAGTTGTGACTCACTATTCCCAGTTGTGCCAAAAGGCTGGCGTTAAGTACAAAAGCTGTGATGGTGATCTTGTTGGTGTTGTGGAGGATTCACAATTTTATTATTGCAAGAAAGATAAGAGAAGTTTTGCAAAAGGGCTATATGATGATGATGATGGAAGGCTGAGAAATTATGGTTTGGTTGATGCAGTTGTTGACTTTCTCAAAAGCAATTCAAAGTTATATTTTCTGAGTGCTATCCAGGATCAGCTACCCCACCTTTTTGGAGGCTCTTCACCTTGCTCATACCGAGGGCTTCGAGAAATGGTGGCTGTGTATATGTGCCATGTACCCGAGTTCTCAACTTCTCGTCACAAAGGATATTTTGAATATGATCTGCATATTACAAACTTGATTGGGATCATGAGAATAAATGCCCAGAATCTACTTGTTCACATGCATGAGAAGCAAATTGAAGCCAAATCGTCAGCCCTGCATTGTTCTGGTTTCATTAATTCAATTATCAATGTTGGTATTCAAACCATTGTGCCATCATTTAGTCATGCCTGGGATTTTCAAATTCATCAACTTGGGCCACCCTTGCCTACTTCTGGTCATTTTGAGGTGATAAACTTTGCAGAGGGGCTTCTTGCTTTCCTGATCTATTTTTCAATTAGTACCATTGGTTCCAAGATGACAATCATTGCTGCCATAGGTGAAATCTGCAATCAGTTACTACAACCTCAAATATGCAATTCCATATTTACCGCAGATTCTACCAAACTGAGCCCATTTGTGCTTGTTATCTTGGACATTGAGCTGCACAATCAAGAGAGTTTGTCTAGACCATTATTTGGTGCTAATTTGCTTCTAAGTTCTTCTTTGGGTAGTGATGATTTCTTGTCTGCAATGTTCAATTTTTTTCATCACTGGCTTTGCCTTGTTCTTGTCCAAACATTTGCAAAATTAGAAGGCTTGTTGAATCATGTTCTAGAGGCCCCAGGACTTCATGAATGGTTGGTCAGAACTATTGAAATTGGAAATCCTTATGCCTTGCTTTTGGCTCAGAAAATGGGAGAAGAAGTTGATGTTCTGTTTTTTCTCGTGCCGAGGTTGCCTGCTGCTTATATACCAATTTGTTTGTCTTGCAAAGTTGTTCAGTGCATTACTGCTGTGATTGCCGTTCCTGTGATATTGGTTTGTGCTGTGTTTCTAACTGCAGTTTATGTCCGCTACAAGTTACGTGACTGTCGTTGCACAGAAATAGATGATTATTTTCACAAGATTGGTCATCCTGGTATGGTAGTGGAGTTAATAAATGAATCAAAACAGGAGGTTCCTAATTGGCTTATTAATCATGCTCAACAATCGTTGTACTATGGTGGAGGCCAAAGCCAAGGTTATATATGTCTCTTGAATCACAGAATGGAGCTCCTTTGCATGCCACATAGTGAGATAGCCATCTTTTGGATTTGGCTTCCTACACCACCGCTACGTTTAAAGGTTTGGGTACTTAACCGGCATGGTTTGATTGTGATCAAGGCTCAAATGTTAATTGGTGATGCATTCTTGAAAATAGAGCTCATGTTTGATAATGAATCTCACATCATTCCCACGGGGGCAATTGCTATTAGACTTGTTTGTCATTTTGGTGCAGATTTGCAGGTTACCAACTCATATTCACACATGGATCACTCTGAAATGTGTTGGCTGCACACATGGCTCATTTGTTGCTTATTATTCTGTGATTGCAGAAAGAAAGCCCTTCCACTTGCCGGCAAGGGAAAACTCAAAGAAATGGCTGCATTATTCAACTGGTTCTTGTGTGCAAGTTCAGAATTTGGCACTTTGGCCACTCTAAATGCATGGTTGAATGGTAATTTTCTGTTTTTACGTCTTGAGAACAAATTGCAGAAAATGAAATTGGTTCTTGCTGATAAGTTGAAGAAACTGGACACAATTGTGAAAATGATGTTTGTTGACAATTGGGAGAGCTGGAAGTATTTAATAATTGCTGCAGATGTCAAGGCTATTGCTGCAAGGGTCTCATATGGGGGTCTATCCGAACTTGTTGCAAGTGAGTATTTTTCTTGCTTGGTGGGGCCAATTATTGCAGGAGAAATGGGTGTATATGGTTATAAACCCTCAGTTCTAATGGATATGCTTGCGGGTTGGCTACATGTTGCTTTGAGGGACAAAGAATTGATCAAGGCAAATTCTACATATGAGAGTGTGACTGCAGCAAGGGTTCATTTTGATCAGTTCAAAGCATTCATCAGAGGTGTCCATATTTTACTCAGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH Peptidase_M16 Peptidase_M16_C

Protein Analysis

1230

Amino Acids

137.49

Weight (kDa)

6.07

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3525
AasI GACNNNNNNGTC 1 cut(s) 3027
Acc36I ACCTGC 3 cut(s) 528, 1081, 3040
AccB1I GGYRCC 1 cut(s) 294
AccB7I CCANNNNNTGG 3 cut(s) 571, 2240, 2853
AccBSI CCGCTC 1 cut(s) 615
AccI GTMKAC 1 cut(s) 1095
AccII CGCG 1 cut(s) 569
AclWI GGATC 5 cut(s) 244, 257, 1659, 1814, 3084
AcoI YGGCCR 2 cut(s) 501, 3237
AcsI RAATTY 6 cut(s) 754, 1258, 1316, 1965, 3224, 3601
AcvI CACGTG 1 cut(s) 21
AcyI GRCGYC 1 cut(s) 295
AdeI CACNNNGTG 1 cut(s) 2837
AfaI GTAC 8 cut(s) 54, 133, 605, 1457, 1737, 2065, 2762, 2898
AfeI AGCGCT 1 cut(s) 178
AfiI CCNNNNNNNGG 7 cut(s) 163, 571, 600, 1997, 2240, 2853, 3161
AflIII ACRYGT 3 cut(s) 20, 567, 3565
AhdI GACNNNNNGTC 1 cut(s) 554
AjnI CCWGG 4 cut(s) 1647, 1952, 2386, 2680
AjuI GAANNNNNNNTTGG 2 cut(s) 3293, 3325
AleI CACNNNNGTG 1 cut(s) 3347
AloI GAACNNNNNNTCC 2 cut(s) 1231, 1263
Alw21I GWGCWC 4 cut(s) 20, 372, 2820, 2970
Alw26I GTCTC 9 cut(s) 176, 451, 518, 625, 703, 969, 1347, 2801, 3436
Alw44I GTGCAC 1 cut(s) 16
AlwI GGATC 5 cut(s) 244, 257, 1659, 1814, 3084
AlwNI CAGNNNCTG 1 cut(s) 3690
Ama87I CYCGRG 1 cut(s) 1739
Aor51HI AGCGCT 1 cut(s) 178
AoxI GGCC 9 cut(s) 167, 270, 398, 501, 1979, 2382, 2773, 3237, 3488
ApaLI GTGCAC 1 cut(s) 16
ApoI RAATTY 6 cut(s) 754, 1258, 1316, 1965, 3224, 3601
AseI ATTAAT 2 cut(s) 1899, 2739
AspLEI GCGC 2 cut(s) 179, 297
AspS9I GGNCC 7 cut(s) 167, 413, 468, 948, 1979, 2383, 3488
AvaI CYCGRG 1 cut(s) 1739
AvaII GGWCC 3 cut(s) 413, 468, 948
BaeGI GKGCMC 1 cut(s) 20
BalI TGGCCA 1 cut(s) 3239
BamHI GGATCC 1 cut(s) 249
BanI GGYRCC 1 cut(s) 294
BanII GRGCYC 5 cut(s) 157, 372, 2177, 2820, 2970
BauI CACGAG 1 cut(s) 2489
BbrPI CACGTG 1 cut(s) 21
BbsI GAAGAC 2 cut(s) 1316, 1383
Bbv12I GWGCWC 4 cut(s) 20, 372, 2820, 2970
BbvCI CCTCAGC 1 cut(s) 350
BccI CCATC 9 cut(s) 379, 559, 592, 760, 1265, 1463, 1556, 1945, 2855
BceAI ACGGC 3 cut(s) 282, 376, 2552
BcgI CGANNNNNNTGC 2 cut(s) 3440, 3474
BciT130I CCWGG 4 cut(s) 1649, 1954, 2388, 2682
BclI TGATCA 3 cut(s) 2922, 3591, 3643
BcoDI GTCTC 9 cut(s) 176, 451, 518, 625, 703, 969, 1347, 2801, 3436
BfaI CTAG 3 cut(s) 546, 2229, 2378
BfmI CTRYAG 3 cut(s) 2604, 3402, 3624
BfoI RGCGCY 2 cut(s) 180, 298
BfuAI ACCTGC 3 cut(s) 528, 1081, 3040
BglI GCCNNNNNGGC 1 cut(s) 397
BlpI GCTNAGC 2 cut(s) 825, 866
Bme1390I CCNGG 4 cut(s) 1649, 1954, 2388, 2682
Bme18I GGWCC 3 cut(s) 413, 468, 948
BmeRI GACNNNNNGTC 1 cut(s) 554
BmeT110I CYCGRG 1 cut(s) 1739
BmgT120I GGNCC 7 cut(s) 167, 413, 468, 948, 1979, 2383, 3488
BmiI GGNNCC 9 cut(s) 168, 251, 296, 390, 469, 2074, 2385, 2724, 3489
BmrFI CCNGG 4 cut(s) 1649, 1954, 2388, 2682
BmrI ACTGGG 1 cut(s) 1423
BmsI GCATC 3 cut(s) 1006, 1582, 2938
BmuI ACTGGG 1 cut(s) 1423
BpiI GAAGAC 2 cut(s) 1316, 1383
Bpu10I CCTNAGC 2 cut(s) 350, 378
Bpu1102I GCTNAGC 2 cut(s) 825, 866
BpuEI CTTGAG 1 cut(s) 3305
Bsa29I ATCGAT 1 cut(s) 68
BsaAI YACGTR 2 cut(s) 21, 2630
BsaBI GATNNNNATC 6 cut(s) 636, 1473, 1805, 2084, 2846, 2921
BsaHI GRCGYC 1 cut(s) 295
BsaI GGTCTC 2 cut(s) 518, 3436
BsaJI CCNNGG 8 cut(s) 170, 405, 1696, 1953, 2386, 2495, 2782, 3000
BsaWI WCCGGW 1 cut(s) 327
BsaXI ACNNNNNCTCC 4 cut(s) 175, 205, 2454, 2484
Bsc4I CCNNNNNNNGG 7 cut(s) 163, 571, 600, 1997, 2240, 2853, 3161
Bse118I RCCGGY 3 cut(s) 499, 2904, 3161
Bse1I ACTGG 7 cut(s) 148, 416, 431, 1429, 2321, 3206, 3342
Bse3DI GCAATG 3 cut(s) 877, 2088, 2301
Bse8I GATNNNNATC 6 cut(s) 636, 1473, 1805, 2084, 2846, 2921
BseBI CCWGG 4 cut(s) 1649, 1954, 2388, 2682
BseCI ATCGAT 1 cut(s) 68
BseDI CCNNGG 8 cut(s) 170, 405, 1696, 1953, 2386, 2495, 2782, 3000
BseGI GGATG 4 cut(s) 584, 1276, 1288, 2677
BseJI GATNNNNATC 6 cut(s) 636, 1473, 1805, 2084, 2846, 2921
BseLI CCNNNNNNNGG 7 cut(s) 163, 571, 600, 1997, 2240, 2853, 3161
BseMI GCAATG 3 cut(s) 877, 2088, 2301
BseNI ACTGG 7 cut(s) 148, 416, 431, 1429, 2321, 3206, 3342
BseRI GAGGAG 3 cut(s) 187, 253, 439
BseSI GKGCMC 1 cut(s) 20
BseYI CCCAGC 1 cut(s) 589
BsgI GTGCAG 5 cut(s) 45, 1428, 2192, 3060, 3083
Bsh1236I CGCG 1 cut(s) 569
Bsh1285I CGRYCG 1 cut(s) 365
BshFI GGCC 9 cut(s) 169, 272, 400, 503, 1981, 2384, 2775, 3239, 3490
BshNI GGYRCC 1 cut(s) 294
BshVI ATCGAT 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 365
BsiHKAI GWGCWC 4 cut(s) 20, 372, 2820, 2970
BsiHKCI CYCGRG 1 cut(s) 1739
BsiSI CCGG 4 cut(s) 328, 500, 2905, 3162
BslFI GGGAC 2 cut(s) 481, 3593
BslI CCNNNNNNNGG 7 cut(s) 163, 571, 600, 1997, 2240, 2853, 3161
BsmAI GTCTC 9 cut(s) 176, 451, 518, 625, 703, 969, 1347, 2801, 3436
BsmFI GGGAC 2 cut(s) 481, 3593
BsmI GAATGC 3 cut(s) 1159, 2951, 3656
BsnI GGCC 9 cut(s) 169, 272, 400, 503, 1981, 2384, 2775, 3239, 3490
Bso31I GGTCTC 2 cut(s) 518, 3436
BsoBI CYCGRG 1 cut(s) 1739
Bsp1286I GDGCHC 6 cut(s) 20, 157, 372, 2177, 2820, 2970
Bsp1720I GCTNAGC 2 cut(s) 825, 866
BspANI GGCC 9 cut(s) 169, 272, 400, 503, 1981, 2384, 2775, 3239, 3490
BspDI ATCGAT 1 cut(s) 68
BspFNI CGCG 1 cut(s) 569
BspHI TCATGA 3 cut(s) 1396, 1809, 2395
BspLI GGNNCC 9 cut(s) 168, 251, 296, 390, 469, 2074, 2385, 2724, 3489
BspMAI CTGCAG 3 cut(s) 2608, 3406, 3628
BspMI ACCTGC 3 cut(s) 528, 1081, 3040
BspPI GGATC 5 cut(s) 244, 257, 1659, 1814, 3084
BspQI GCTCTTC 1 cut(s) 1684
BspT107I GGYRCC 1 cut(s) 294
BspTNI GGTCTC 2 cut(s) 518, 3436
BsrBI CCGCTC 1 cut(s) 615
BsrDI GCAATG 3 cut(s) 877, 2088, 2301
BsrFI RCCGGY 3 cut(s) 499, 2904, 3161
BsrI ACTGG 7 cut(s) 148, 416, 431, 1429, 2321, 3206, 3342
BssAI RCCGGY 3 cut(s) 499, 2904, 3161
BssECI CCNNGG 8 cut(s) 170, 405, 1696, 1953, 2386, 2495, 2782, 3000
BssNI GRCGYC 1 cut(s) 295
BssSI CACGAG 1 cut(s) 2489
BssT1I CCWWGG 1 cut(s) 2782
Bst2BI CACGAG 1 cut(s) 2489
Bst2UI CCWGG 4 cut(s) 1649, 1954, 2388, 2682
Bst4CI ACNGT 3 cut(s) 562, 903, 2636
Bst6I CTCTTC 2 cut(s) 239, 1684
BstACI GRCGYC 1 cut(s) 295
BstBAI YACGTR 2 cut(s) 21, 2630
BstC8I GCNNGC 7 cut(s) 1334, 1338, 1447, 2505, 2828, 3163, 3552
BstDSI CCRYGG 1 cut(s) 3000
BstEII GGTNACC 1 cut(s) 3052
BstF5I GGATG 4 cut(s) 584, 1276, 1288, 2677
BstFNI CGCG 1 cut(s) 569
BstH2I RGCGCY 2 cut(s) 180, 298
BstHHI GCGC 2 cut(s) 179, 297
BstMAI GTCTC 9 cut(s) 176, 451, 518, 625, 703, 969, 1347, 2801, 3436
BstMCI CGRYCG 1 cut(s) 365
BstMWI GCNNNNNNNGC 8 cut(s) 281, 397, 1852, 1987, 2500, 2564, 3106, 3422
BstNI CCWGG 4 cut(s) 1649, 1954, 2388, 2682
BstNSI RCATGY 4 cut(s) 1387, 1846, 2830, 3569
BstPI GGTNACC 1 cut(s) 3052
BstSCI CCNGG 4 cut(s) 1647, 1952, 2386, 2680
BstSFI CTRYAG 3 cut(s) 2604, 3402, 3624
BstSLI GKGCMC 1 cut(s) 20
BstUI CGCG 1 cut(s) 569
BstV2I GAAGAC 2 cut(s) 1316, 1383
BstX2I RGATCY 1 cut(s) 249
BstXI CCANNNNNNTGG 3 cut(s) 1388, 1445, 1670
BstYI RGATCY 1 cut(s) 249
Bsu15I ATCGAT 1 cut(s) 68
BsuRI GGCC 9 cut(s) 169, 272, 400, 503, 1981, 2384, 2775, 3239, 3490
BsuTUI ATCGAT 1 cut(s) 68
BtgI CCRYGG 1 cut(s) 3000
BtsCI GGATG 4 cut(s) 584, 1276, 1288, 2677
BtsI GCAGTG 2 cut(s) 33, 826
BtsIMutI CAGTG 6 cut(s) 33, 438, 826, 1054, 2314, 2552
BveI ACCTGC 3 cut(s) 528, 1081, 3040
Cac8I GCNNGC 7 cut(s) 1334, 1338, 1447, 2505, 2828, 3163, 3552
CaiI CAGNNNCTG 1 cut(s) 3690
CciI TCATGA 3 cut(s) 1396, 1809, 2395
CfoI GCGC 2 cut(s) 179, 297
Cfr10I RCCGGY 3 cut(s) 499, 2904, 3161
Cfr13I GGNCC 7 cut(s) 167, 413, 468, 948, 1979, 2383, 3488
ClaI ATCGAT 1 cut(s) 68
CseI GACGC 3 cut(s) 203, 309, 1070
Csp6I GTAC 8 cut(s) 53, 132, 604, 1456, 1736, 2064, 2761, 2897
CspCI CAANNNNNGTGG 2 cut(s) 967, 1002
CviQI GTAC 8 cut(s) 53, 132, 604, 1456, 1736, 2064, 2761, 2897
DinI GGCGCC 1 cut(s) 296
DraI TTTAAA 1 cut(s) 2886
DraIII CACNNNGTG 1 cut(s) 2837
DrdI GACNNNNNNGTC 1 cut(s) 3027
DriI GACNNNNNGTC 1 cut(s) 554
DseDI GACNNNNNNGTC 1 cut(s) 3027
EaeI YGGCCR 2 cut(s) 501, 3237
Eam1104I CTCTTC 2 cut(s) 239, 1684
Eam1105I GACNNNNNGTC 1 cut(s) 554
EarI CTCTTC 2 cut(s) 239, 1684
EciI GGCGGA 1 cut(s) 525
Ecl136II GAGCTC 3 cut(s) 370, 2818, 2968
Eco130I CCWWGG 1 cut(s) 2782
Eco24I GRGCYC 5 cut(s) 157, 372, 2177, 2820, 2970
Eco31I GGTCTC 2 cut(s) 518, 3436
Eco32I GATATC 1 cut(s) 425
Eco47I GGWCC 3 cut(s) 413, 468, 948
Eco47III AGCGCT 1 cut(s) 178
Eco53kI GAGCTC 3 cut(s) 370, 2818, 2968
Eco72I CACGTG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 1739
Eco91I GGTNACC 1 cut(s) 3052
EcoICRI GAGCTC 3 cut(s) 370, 2818, 2968
EcoO109I RGGNCCY 1 cut(s) 2383
EcoO65I GGTNACC 1 cut(s) 3052
EcoRI GAATTC 1 cut(s) 754
EcoRII CCWGG 4 cut(s) 1647, 1952, 2386, 2680
EcoRV GATATC 1 cut(s) 425
EcoT14I CCWWGG 1 cut(s) 2782
EcoT22I ATGCAT 3 cut(s) 1848, 2953, 3253
EcoT38I GRGCYC 5 cut(s) 157, 372, 2177, 2820, 2970
EgeI GGCGCC 1 cut(s) 296
EheI GGCGCC 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 2782
FalI AAGNNNNNCTT 2 cut(s) 2251, 2283
FaqI GGGAC 2 cut(s) 481, 3593
FauI CCCGC 2 cut(s) 502, 3547
FauNDI CATATG 2 cut(s) 3436, 3610
FbaI TGATCA 3 cut(s) 2922, 3591, 3643
FblI GTMKAC 1 cut(s) 1095
FokI GGATG 4 cut(s) 571, 1283, 1295, 2664
FriOI GRGCYC 5 cut(s) 157, 372, 2177, 2820, 2970
FspBI CTAG 3 cut(s) 546, 2229, 2378
GlaI GCGC 2 cut(s) 178, 296
GsaI CCCAGC 1 cut(s) 593
HaeII RGCGCY 2 cut(s) 180, 298
HaeIII GGCC 9 cut(s) 169, 272, 400, 503, 1981, 2384, 2775, 3239, 3490
HapII CCGG 4 cut(s) 328, 500, 2905, 3162
HgaI GACGC 3 cut(s) 203, 309, 1070
HhaI GCGC 2 cut(s) 179, 297
Hin1I GRCGYC 1 cut(s) 295
Hin6I GCGC 2 cut(s) 177, 295
HinP1I GCGC 2 cut(s) 177, 295
HincII GTYRAC 2 cut(s) 1603, 3367
HindII GTYRAC 2 cut(s) 1603, 3367
HpaII CCGG 4 cut(s) 328, 500, 2905, 3162
Hpy99I CGWCG 2 cut(s) 325, 397
HpyAV CCTTC 5 cut(s) 920, 939, 1560, 2351, 3161
HpyCH4III ACNGT 3 cut(s) 562, 903, 2636
HpyCH4IV ACGT 4 cut(s) 20, 2629, 2881, 3280
HpyF10VI GCNNNNNNNGC 8 cut(s) 281, 397, 1852, 1987, 2500, 2564, 3106, 3422
HpySE526I ACGT 4 cut(s) 20, 2629, 2881, 3280
Hsp92I GRCGYC 1 cut(s) 295
HspAI GCGC 2 cut(s) 177, 295
KasI GGCGCC 1 cut(s) 294
KroI GCCGGC 1 cut(s) 3161
KroNI GCCGGC 1 cut(s) 3163
Ksp22I TGATCA 3 cut(s) 2922, 3591, 3643
LguI GCTCTTC 1 cut(s) 1684
LmnI GCTCC 6 cut(s) 174, 367, 388, 859, 2815, 2823
LweI GCATC 3 cut(s) 1006, 1582, 2938
MaeI CTAG 3 cut(s) 546, 2229, 2378
MaeII ACGT 4 cut(s) 20, 2629, 2881, 3280
MbiI CCGCTC 1 cut(s) 615
MfeI CAATTG 4 cut(s) 624, 3009, 3345, 3370
MflI RGATCY 1 cut(s) 249
MhlI GDGCHC 6 cut(s) 20, 157, 372, 2177, 2820, 2970
MlsI TGGCCA 1 cut(s) 3239
MluI ACGCGT 1 cut(s) 567
MluNI TGGCCA 1 cut(s) 3239
Mly113I GGCGCC 1 cut(s) 295
MlyI GAGTC 6 cut(s) 178, 430, 587, 700, 984, 1411
MmeI TCCRAC 1 cut(s) 922
Mox20I TGGCCA 1 cut(s) 3239
Mph1103I ATGCAT 3 cut(s) 1848, 2953, 3253
MroNI GCCGGC 1 cut(s) 3161
MscI TGGCCA 1 cut(s) 3239
MslI CAYNNNNRTG 4 cut(s) 1194, 1845, 3036, 3347
Msp20I TGGCCA 1 cut(s) 3239
MspA1I CMGCKG 5 cut(s) 211, 275, 287, 509, 865
MspI CCGG 4 cut(s) 328, 500, 2905, 3162
MspR9I CCNGG 4 cut(s) 1649, 1954, 2388, 2682
MunI CAATTG 4 cut(s) 624, 3009, 3345, 3370
Mva1269I GAATGC 3 cut(s) 1159, 2951, 3656
MvaI CCWGG 4 cut(s) 1649, 1954, 2388, 2682
MvnI CGCG 1 cut(s) 569
MwoI GCNNNNNNNGC 8 cut(s) 281, 397, 1852, 1987, 2500, 2564, 3106, 3422
NaeI GCCGGC 1 cut(s) 3163
NarI GGCGCC 1 cut(s) 295
NdeI CATATG 2 cut(s) 3436, 3610
NgoMIV GCCGGC 1 cut(s) 3161
NlaIV GGNNCC 9 cut(s) 168, 251, 296, 390, 469, 2074, 2385, 2724, 3489
NmeAIII GCCGAG 3 cut(s) 195, 386, 2520
NmuCI GTSAC 8 cut(s) 433, 556, 579, 712, 1414, 1757, 2630, 3619
NsiI ATGCAT 3 cut(s) 1848, 2953, 3253
NspI RCATGY 4 cut(s) 1387, 1846, 2830, 3569
OliI CACNNNNGTG 1 cut(s) 3347
PaeI GCATGC 1 cut(s) 2830
PagI TCATGA 3 cut(s) 1396, 1809, 2395
PciI ACATGT 1 cut(s) 3565
PciSI GCTCTTC 1 cut(s) 1684
PcsI WCGNNNNNNNCGW 2 cut(s) 127, 2635
PctI GAATGC 3 cut(s) 1159, 2951, 3656
PdiI GCCGGC 1 cut(s) 3163
PflMI CCANNNNNTGG 3 cut(s) 571, 2240, 2853
PfoI TCCNGGA 1 cut(s) 1647
Ple19I CGATCG 1 cut(s) 365
PleI GAGTC 6 cut(s) 178, 430, 586, 699, 984, 1411
PluTI GGCGCC 1 cut(s) 298
PmaCI CACGTG 1 cut(s) 21
PmlI CACGTG 1 cut(s) 21
PpsI GAGTC 6 cut(s) 178, 430, 586, 699, 984, 1411
Ppu21I YACGTR 2 cut(s) 21, 2630
PscI ACATGT 1 cut(s) 3565
PshBI ATTAAT 2 cut(s) 1899, 2739
PsiI TTATAA 1 cut(s) 3525
Psp124BI GAGCTC 3 cut(s) 372, 2820, 2970
Psp6I CCWGG 4 cut(s) 1647, 1952, 2386, 2680
PspCI CACGTG 1 cut(s) 21
PspEI GGTNACC 1 cut(s) 3052
PspFI CCCAGC 1 cut(s) 589
PspGI CCWGG 4 cut(s) 1647, 1952, 2386, 2680
PspN4I GGNNCC 9 cut(s) 168, 251, 296, 390, 469, 2074, 2385, 2724, 3489
PspPI GGNCC 7 cut(s) 167, 413, 468, 948, 1979, 2383, 3488
PsrI GAACNNNNNNTAC 2 cut(s) 2056, 2088
PstI CTGCAG 3 cut(s) 2608, 3406, 3628
PstNI CAGNNNCTG 1 cut(s) 3690
PsuI RGATCY 1 cut(s) 249
PvuI CGATCG 1 cut(s) 365
PvuII CAGCTG 2 cut(s) 287, 865
RsaI GTAC 8 cut(s) 54, 133, 605, 1457, 1737, 2065, 2762, 2898
RsaNI GTAC 8 cut(s) 53, 132, 604, 1456, 1736, 2064, 2761, 2897
RseI CAYNNNNRTG 4 cut(s) 1194, 1845, 3036, 3347
SacI GAGCTC 3 cut(s) 372, 2820, 2970
SapI GCTCTTC 1 cut(s) 1684
Sau96I GGNCC 7 cut(s) 167, 413, 468, 948, 1979, 2383, 3488
SchI GAGTC 6 cut(s) 178, 430, 587, 700, 984, 1411
ScrFI CCNGG 4 cut(s) 1649, 1954, 2388, 2682
SduI GDGCHC 6 cut(s) 20, 157, 372, 2177, 2820, 2970
SfaNI GCATC 3 cut(s) 1006, 1582, 2938
SfcI CTRYAG 3 cut(s) 2604, 3402, 3624
SfoI GGCGCC 1 cut(s) 296
SinI GGWCC 3 cut(s) 413, 468, 948
SmiMI CAYNNNNRTG 4 cut(s) 1194, 1845, 3036, 3347
SmlI CTYRAG 1 cut(s) 3284
SmoI CTYRAG 1 cut(s) 3284
SphI GCATGC 1 cut(s) 2830
SspDI GGCGCC 1 cut(s) 294
SspMI CTAG 3 cut(s) 546, 2229, 2378
SstI GAGCTC 3 cut(s) 372, 2820, 2970
StyD4I CCNGG 4 cut(s) 1647, 1952, 2386, 2680
StyI CCWWGG 1 cut(s) 2782
TaaI ACNGT 3 cut(s) 562, 903, 2636
TaiI ACGT 4 cut(s) 23, 2632, 2884, 3283
TaqI TCGA 5 cut(s) 68, 361, 395, 973, 1705
TatI WGTACW 3 cut(s) 52, 1455, 2760
TauI GCSGC 2 cut(s) 275, 541
TscAI CASTG 6 cut(s) 33, 438, 826, 1054, 2321, 2552
TseFI GTSAC 8 cut(s) 433, 556, 579, 712, 1414, 1757, 2630, 3619
Tsp45I GTSAC 8 cut(s) 433, 556, 579, 712, 1414, 1757, 2630, 3619
TspRI CASTG 6 cut(s) 33, 438, 826, 1054, 2321, 2552
Van91I CCANNNNNTGG 3 cut(s) 571, 2240, 2853
VneI GTGCAC 1 cut(s) 16
VpaK11BI GGWCC 3 cut(s) 413, 468, 948
VspI ATTAAT 2 cut(s) 1899, 2739
XapI RAATTY 6 cut(s) 754, 1258, 1316, 1965, 3224, 3601
XbaI TCTAGA 2 cut(s) 2228, 2377
XceI RCATGY 4 cut(s) 1387, 1846, 2830, 3569
XmiI GTMKAC 1 cut(s) 1095
XspI CTAG 3 cut(s) 546, 2229, 2378
Zsp2I ATGCAT 3 cut(s) 1848, 2953, 3253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.