Rroxscaffold_4G00276460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005168
Physical Location & Seq
Forward (+)
47605 .. 49256
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00276460.1

Sequence Viewer

Length: 870 bp
ATGGGGCCGAGCAGCCTTGGGACTCCACCACCAACCTCGTTCGCACCGCCGCGTCCACCCGACCTCGATGAAGTGAGGAGGCGGCGAATCCACATTGCCAAAGAACAAGTAGCGGAAAGGACACCGCATGGCGCCGTGAACTCGAGAAAACTATGGCGTCGTTCCGTCCGAGTTGTTAAACGAGTTGAGGCACTTTCGATTGGAGCTCACGCCTCGTGTGATGGAGTCGTCGAGTTTCGCAAGCCCGGGTTGGTGCTTGGAGATTGTGAGCTTATTCATGACGAAGACCACATTCTTCAAGATCATAAGCAAAGTGTAACTATGACCGGCTTTCCCCAATTATTGTGGGATGAGGAAAACTCTTCTCGTGTAACCATTTTCAATCCTAGGTTGGAAGCTACTTTCTTGAATCGAAACTTGGTTGAATCATTTGCGACAATAGCGGTGTCAATGTTTGATTTCTCTAAGCATGCAAGGAAACACAAGGCCATTGATTTTGAGCAGTTCATATTGTGTCATTTTGGCATTAGTGAATTAATGGCAACTCTTGAAGGAGCAAACTTACAATCTAGTCTACCTAACTGGAAATTTAGATTAAAATTTTCAACTTCACGTCACAAAGGATATTTTGAATATGATCTGCATATTACAAACTTGACTGGGAGCATGAGAATAAATGCCCGTAATCTATTTGTTCACGTGCATGAGAGGCAAATTGAAGCCAAATCTTCAGCCCTACATTTTTCTAGTTTCATTAATTCAATTATCAATGCTGGTATTGAGACCATTGTGCCATCATTTAGTCATGCTCGGGATTTTCAAATTCACCAACTTGGACCACCCTTGCCTACTTCGGTCATTTTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

289

Amino Acids

32.77

Weight (kDa)

9.24

Isoelectric Point (pI)

59.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 131
AccI GTMKAC 1 cut(s) 574
AccII CGCG 1 cut(s) 52
AciI CCGC 6 cut(s) 47, 50, 82, 113, 125, 443
AcsI RAATTY 3 cut(s) 587, 599, 822
AcuI CTGAAG 1 cut(s) 714
AcvI CACGTG 1 cut(s) 700
AcyI GRCGYC 2 cut(s) 132, 157
AjiI CACGTC 1 cut(s) 614
AjuI GAANNNNNNNTTGG 2 cut(s) 401, 433
AluBI AGCT 3 cut(s) 206, 271, 398
AluI AGCT 3 cut(s) 206, 271, 398
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 1 cut(s) 776
Ama87I CYCGRG 3 cut(s) 142, 245, 810
AoxI GGCC 2 cut(s) 5, 486
ApeKI GCWGC 1 cut(s) 12
ApoI RAATTY 3 cut(s) 587, 599, 822
AseI ATTAAT 2 cut(s) 536, 756
AspA2I CCTAGG 1 cut(s) 386
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 2 cut(s) 5, 836
AsuC2I CCSGG 2 cut(s) 246, 247
AsuHPI GGTGA 1 cut(s) 818
AvaI CYCGRG 3 cut(s) 142, 245, 810
AvaII GGWCC 1 cut(s) 836
AvrII CCTAGG 1 cut(s) 386
BanI GGYRCC 1 cut(s) 131
BanII GRGCYC 1 cut(s) 208
BauI CACGAG 2 cut(s) 214, 366
BbrPI CACGTG 1 cut(s) 700
BbsI GAAGAC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 1 cut(s) 24
BccI CCATC 2 cut(s) 215, 802
BceAI ACGGC 1 cut(s) 119
BcnI CCSGG 2 cut(s) 246, 247
BcoDI GTCTC 1 cut(s) 776
BfaI CTAG 3 cut(s) 387, 570, 747
BfoI RGCGCY 1 cut(s) 135
BisI GCNGC 3 cut(s) 13, 50, 83
BlnI CCTAGG 1 cut(s) 386
BlsI GCNGC 3 cut(s) 14, 51, 84
Bme1390I CCNGG 2 cut(s) 246, 247
Bme18I GGWCC 1 cut(s) 836
BmeT110I CYCGRG 3 cut(s) 142, 245, 810
BmgBI CACGTC 1 cut(s) 614
BmgT120I GGNCC 2 cut(s) 5, 836
BmiI GGNNCC 2 cut(s) 6, 133
BmrFI CCNGG 2 cut(s) 246, 247
BmrI ACTGGG 1 cut(s) 669
BmuI ACTGGG 1 cut(s) 669
BpiI GAAGAC 1 cut(s) 291
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpuMI CCSGG 2 cut(s) 246, 247
BsaAI YACGTR 1 cut(s) 700
BsaHI GRCGYC 2 cut(s) 132, 157
BsaI GGTCTC 1 cut(s) 776
BsaJI CCNNGG 3 cut(s) 16, 245, 386
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 2 cut(s) 587, 664
Bse3DI GCAATG 1 cut(s) 93
BseDI CCNNGG 3 cut(s) 16, 245, 386
BseGI GGATG 1 cut(s) 355
BseMI GCAATG 1 cut(s) 93
BseNI ACTGG 2 cut(s) 587, 664
BseRI GAGGAG 1 cut(s) 91
BseXI GCAGC 1 cut(s) 24
Bsh1236I CGCG 1 cut(s) 52
BshFI GGCC 2 cut(s) 7, 488
BshNI GGYRCC 1 cut(s) 131
BsiHKAI GWGCWC 1 cut(s) 208
BsiHKCI CYCGRG 3 cut(s) 142, 245, 810
BsiSI CCGG 2 cut(s) 246, 327
BslFI GGGAC 1 cut(s) 34
BsmAI GTCTC 1 cut(s) 776
BsmFI GGGAC 1 cut(s) 34
BsnI GGCC 2 cut(s) 7, 488
Bso31I GGTCTC 1 cut(s) 776
BsoBI CYCGRG 3 cut(s) 142, 245, 810
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 2 cut(s) 301, 637
BspACI CCGC 6 cut(s) 47, 50, 82, 113, 125, 443
BspANI GGCC 2 cut(s) 7, 488
BspFNI CGCG 1 cut(s) 52
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 2 cut(s) 6, 133
BspT107I GGYRCC 1 cut(s) 131
BspTNI GGTCTC 1 cut(s) 776
BsrDI GCAATG 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 2 cut(s) 587, 664
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 3 cut(s) 16, 245, 386
BssMI GATC 2 cut(s) 301, 637
BssNI GRCGYC 2 cut(s) 132, 157
BssSI CACGAG 2 cut(s) 214, 366
BssT1I CCWWGG 2 cut(s) 16, 386
Bst2BI CACGAG 2 cut(s) 214, 366
Bst6I CTCTTC 1 cut(s) 367
BstACI GRCGYC 2 cut(s) 132, 157
BstBAI YACGTR 1 cut(s) 700
BstC8I GCNNGC 2 cut(s) 242, 471
BstDEI CTNAG 1 cut(s) 465
BstF5I GGATG 1 cut(s) 355
BstFNI CGCG 1 cut(s) 52
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 2 cut(s) 304, 640
BstMAI GTCTC 1 cut(s) 776
BstMBI GATC 2 cut(s) 301, 637
BstMWI GCNNNNNNNGC 2 cut(s) 440, 709
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 244, 245
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 1 cut(s) 24
BstV2I GAAGAC 1 cut(s) 291
BsuRI GGCC 2 cut(s) 7, 488
BtrI CACGTC 1 cut(s) 614
BtsCI GGATG 1 cut(s) 355
Cac8I GCNNGC 2 cut(s) 242, 471
CciI TCATGA 1 cut(s) 277
CfoI GCGC 1 cut(s) 134
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 2 cut(s) 5, 836
Cfr9I CCCGGG 1 cut(s) 245
CseI GACGC 2 cut(s) 41, 146
CspCI CAANNNNNGTGG 2 cut(s) 326, 361
CviAII CATG 6 cut(s) 128, 278, 470, 667, 704, 806
DdeI CTNAG 1 cut(s) 465
DinI GGCGCC 1 cut(s) 133
DpnI GATC 2 cut(s) 303, 639
DpnII GATC 2 cut(s) 301, 637
Eam1104I CTCTTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 367
Ecl136II GAGCTC 1 cut(s) 206
Eco130I CCWWGG 2 cut(s) 16, 386
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 1 cut(s) 776
Eco47I GGWCC 1 cut(s) 836
Eco53kI GAGCTC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 714
Eco72I CACGTG 1 cut(s) 700
Eco88I CYCGRG 3 cut(s) 142, 245, 810
EcoICRI GAGCTC 1 cut(s) 206
EcoT14I CCWWGG 2 cut(s) 16, 386
EcoT38I GRGCYC 1 cut(s) 208
EgeI GGCGCC 1 cut(s) 133
EheI GGCGCC 1 cut(s) 133
ErhI CCWWGG 2 cut(s) 16, 386
FaeI CATG 6 cut(s) 131, 281, 473, 670, 707, 809
FaqI GGGAC 1 cut(s) 34
FatI CATG 6 cut(s) 127, 277, 469, 666, 703, 805
FblI GTMKAC 1 cut(s) 574
Fnu4HI GCNGC 3 cut(s) 13, 50, 83
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 1 cut(s) 208
Fsp4HI GCNGC 3 cut(s) 13, 50, 83
FspBI CTAG 3 cut(s) 387, 570, 747
GlaI GCGC 1 cut(s) 133
GluI GCNGC 3 cut(s) 13, 50, 83
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 2 cut(s) 7, 488
HapII CCGG 2 cut(s) 246, 327
HgaI GACGC 2 cut(s) 41, 146
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 2 cut(s) 132, 157
Hin1II CATG 6 cut(s) 131, 281, 473, 670, 707, 809
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HinfI GANTC 5 cut(s) 22, 87, 225, 409, 425
HpaII CCGG 2 cut(s) 246, 327
HphI GGTGA 1 cut(s) 818
Hpy166II GTNNAC 4 cut(s) 56, 139, 575, 697
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 6 cut(s) 144, 278, 299, 406, 548, 812
Hpy8I GTNNAC 4 cut(s) 56, 139, 575, 697
Hpy99I CGWCG 2 cut(s) 162, 233
HpyAV CCTTC 1 cut(s) 545
HpyCH4IV ACGT 2 cut(s) 613, 699
HpyCH4V TGCA 3 cut(s) 473, 643, 703
HpyF10VI GCNNNNNNNGC 2 cut(s) 440, 709
HpyF3I CTNAG 1 cut(s) 465
HpySE526I ACGT 2 cut(s) 613, 699
Hsp92I GRCGYC 2 cut(s) 132, 157
Hsp92II CATG 6 cut(s) 131, 281, 473, 670, 707, 809
HspAI GCGC 1 cut(s) 132
KasI GGCGCC 1 cut(s) 131
Kzo9I GATC 2 cut(s) 301, 637
LmnI GCTCC 3 cut(s) 203, 554, 663
LpnPI CCDG 5 cut(s) 259, 340, 568, 645, 759
Lsp1109I GCAGC 1 cut(s) 24
MaeI CTAG 3 cut(s) 387, 570, 747
MaeII ACGT 2 cut(s) 613, 699
MaeIII GTNAC 3 cut(s) 316, 370, 614
MalI GATC 2 cut(s) 303, 639
MboI GATC 2 cut(s) 301, 637
MboII GAAGA 4 cut(s) 287, 296, 354, 720
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 8 cut(s) 338, 533, 587, 599, 714, 757, 762, 822
Mly113I GGCGCC 1 cut(s) 132
MlyI GAGTC 2 cut(s) 16, 234
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 9 cut(s) 46, 69, 72, 74, 181, 223, 346, 702, 858
MseI TTAA 4 cut(s) 177, 536, 596, 756
MslI CAYNNNNRTG 1 cut(s) 702
MspI CCGG 2 cut(s) 246, 327
MspR9I CCNGG 2 cut(s) 246, 247
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 2 cut(s) 440, 709
NarI GGCGCC 1 cut(s) 132
NciI CCSGG 2 cut(s) 246, 247
NdeII GATC 2 cut(s) 301, 637
NlaIII CATG 6 cut(s) 131, 281, 473, 670, 707, 809
NlaIV GGNNCC 2 cut(s) 6, 133
NmeAIII GCCGAG 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 614
NspI RCATGY 1 cut(s) 473
PaeI GCATGC 1 cut(s) 473
PaeR7I CTCGAG 1 cut(s) 142
PagI TCATGA 1 cut(s) 277
PcsI WCGNNNNNNNCGW 1 cut(s) 166
PfeI GAWTC 3 cut(s) 87, 409, 425
PkrI GCNGC 3 cut(s) 14, 51, 84
PleI GAGTC 2 cut(s) 16, 233
PluTI GGCGCC 1 cut(s) 135
PmaCI CACGTG 1 cut(s) 700
PmlI CACGTG 1 cut(s) 700
PpsI GAGTC 2 cut(s) 16, 233
Ppu21I YACGTR 1 cut(s) 700
PshBI ATTAAT 2 cut(s) 536, 756
Psp124BI GAGCTC 1 cut(s) 208
PspCI CACGTG 1 cut(s) 700
PspN4I GGNNCC 2 cut(s) 6, 133
PspPI GGNCC 2 cut(s) 5, 836
RseI CAYNNNNRTG 1 cut(s) 702
SacI GAGCTC 1 cut(s) 208
SaqAI TTAA 4 cut(s) 177, 536, 596, 756
SatI GCNGC 3 cut(s) 13, 50, 83
Sau3AI GATC 2 cut(s) 301, 637
Sau96I GGNCC 2 cut(s) 5, 836
SchI GAGTC 2 cut(s) 16, 234
ScrFI CCNGG 2 cut(s) 246, 247
SduI GDGCHC 1 cut(s) 208
SfoI GGCGCC 1 cut(s) 133
Sfr274I CTCGAG 1 cut(s) 142
SinI GGWCC 1 cut(s) 836
SlaI CTCGAG 1 cut(s) 142
SmaI CCCGGG 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 702
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
SphI GCATGC 1 cut(s) 473
Sse9I AATT 8 cut(s) 338, 533, 587, 599, 714, 757, 762, 822
SsiI CCGC 6 cut(s) 47, 50, 82, 113, 125, 443
SspDI GGCGCC 1 cut(s) 131
SspMI CTAG 3 cut(s) 387, 570, 747
SstI GAGCTC 1 cut(s) 208
StyD4I CCNGG 2 cut(s) 244, 245
StyI CCWWGG 2 cut(s) 16, 386
TaiI ACGT 2 cut(s) 616, 702
TaqI TCGA 5 cut(s) 66, 143, 197, 231, 412
TaqII GACCGA 1 cut(s) 844
TasI AATT 8 cut(s) 338, 533, 587, 599, 714, 757, 762, 822
TauI GCSGC 2 cut(s) 52, 85
TfiI GAWTC 3 cut(s) 87, 409, 425
Tru1I TTAA 4 cut(s) 177, 536, 596, 756
Tru9I TTAA 4 cut(s) 177, 536, 596, 756
TseFI GTSAC 1 cut(s) 614
TseI GCWGC 1 cut(s) 12
Tsp45I GTSAC 1 cut(s) 614
TspDTI ATGAA 4 cut(s) 84, 266, 496, 742
TspGWI ACGGA 1 cut(s) 154
TspMI CCCGGG 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 836
VspI ATTAAT 2 cut(s) 536, 756
XapI RAATTY 3 cut(s) 587, 599, 822
XceI RCATGY 1 cut(s) 473
XhoI CTCGAG 1 cut(s) 142
XmaI CCCGGG 1 cut(s) 245
XmaJI CCTAGG 1 cut(s) 386
XmiI GTMKAC 1 cut(s) 574
XspI CTAG 3 cut(s) 387, 570, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.