Rroxscaffold_4G00302780

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005168
Physical Location & Seq
Reverse (-)
23137954 .. 23142397
4444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302780.1

Sequence Viewer

Length: 2151 bp
ATGACAGAGAGTTCAAAGGGAAATTTAGATTTGGATGGGCTTTGGGATGATAGTGGTGGATTGTCCACCACGAAGATTAAATTTAAGAGTTTCTGCAAGCCTGGTTTGGTGCTTGGAGACTTTGAGCTGATTCATGTAGAAGACCACATGCTTGGAGATCATGAGCAAAGTGCAGTTATGACTCACTATTCCCAGTGGTGCCAAAAGGCCGGCATCAAGTACAAAAGCTGTGATGATGATCTTGTTCGTGTTGTGGAGGATTCACAATTTTATCATTGCAAGAAAGATAAGAGAAGTTTTGCGAAAGGGCAGGTGTGGGCTCTATATGATGATGATGGGAAGCTGAGAAATTATGGTTTGGTTGATGCAGTTGTTGACTTTCTCAAAAGCAATTCAAAGTTATATTTTATGAGGTACACTTCTGATGCACTTCTTTCAAAAGATAATTGTGTAGGCTTTTTGACTAATATGCACAAGCTTTTTCAACACATGGTGGGACATGTTCATAAGGATGAGCTTTTCATGGCAACGTGCTCTTCCATTCATCCTCCATTCCATGCACTTTTGTCTGACCTATTGAGAGAAGGTGCACAATTTATTTTTGGAATTATGAGGGTTGTCTCTAGGAATGAGCTGAGGTTTTTGAGGGCTAAGAAACGAGGCCTTGAAGTTGAGTATGGTGATATTGTTAATGGAAATTATTGTTCTTCTGTGAGCGGTGCTCTTTACTTTCCTAATTTCATGATTACATTTATCAAATTTGACTTCGTGGCAATTGATGTTGGAGCTCTTGCATTGGTTCCTCAAATACACTTTTCAGTACACATGTGGTCAAATGAAGCTCTTCTACTTATTTTGTTGTCTGCAATTATGCACTCCCAGACAATGGAATTTGTAAATTCAAGCAATCTATTGCAGAGTCCCAATAGAGAAGCCTTGAAGGTCTTTTATAATATGCAAAATTTGTATCATCGGCAGTCAAATTTTAATTTTCATATGAGTGTTCATCCATGTGCACTACTACATACTTGGCAAGTTACTGCAGCTGATGGATACTTGCAATTGGATTATAAATTGGGCTGTGATCTTATGACTATGGACAATGAAAGCGAGTTGGTTATGTATACTGCAATGTTCAATTTTCATCACTGGCTTTGCATTGTTCTTGTCCGAGCATTTGCAAGATCAGAAGTCTTGTTGAATCATGTTCTAGAGTCTCCAGGACTTCACGAATGGTTGGCAAGGGCTATTGAAATTCGAAATCCTAATGCTTTGATTTTGGCTCTGAAAATGGGAGAAAAAGTTTATGTGGCCAGTACAAGATTTGGGAAACTTCTTGCTAATCTGTCTTCTCTTGCTAATAGTTTAATGGACACTGCTTTCTGTCAGCCACACAGCCACAATGTTTGTCCTGTTCAATTACACATTTTATTACCAGTTAGATTTTTGCAGGCTAAAGATGCATTGACTATTGCAAATCCCTTAACGAAGCACAAAAAGAATCGCAAGTCTAGCTCTCCTGATGATTTCGAAGTTGATGATCACCTATTTGATTTCTACAGAATTTTCATTGAACCTCTGATTGCTTGCTACTTGAGATACACAGCTAAGCAGCCCAGCTATGGAAGGTATGAAGATAGTCCTGTGACAGTTTCATCTACCATCGTGAATGGAGAAAAATGTATGTCTGTGACTATTGACAAATTATGCACTGCTTTAGTTTTCAAGTGTTGTCTAATTGGTAGAGTTGAGGTGTCAGGGGAGTGGTTTCTGAATGTGATGAATATGAATTTGGAGACCATGGTTGGAAAAGTTAGATGTGGTAGGGAAAAGGAGTTGCATGTGAATCTCTTTAAAATTCATAGGGAGCCACTTGAGAAATGTGTGGGGTATGCTTTATCTACTTTGAATGCAAGCTATTCACGAGTCCAGAAGCTTTTCGTGGTTGATTGGGCTTTTGCAAATGTACCAGTATCTTGGAGGGATGTGGAAACCAAGTTGTTTGCTCTTAATGTGGTTACTAAGTTGAACCTAATCTCCCATATATTAGTGATCCTGCTAACCCCAAGTGGGCACCTGTTGCTGTGTTGGCTAGGCGAATGTTCAAAAATAGGGGTGCTGCTGGTACTCAGTGGTTGGTGCAATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

716

Amino Acids

81.39

Weight (kDa)

7.16

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 85 - 139 1.6e-06 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 951, 1071
AarI CACCTGC 1 cut(s) 301
Acc36I ACCTGC 1 cut(s) 301
AccB1I GGYRCC 2 cut(s) 198, 2073
AccB7I CCANNNNNTGG 1 cut(s) 886
AccBSI CCGCTC 1 cut(s) 717
AccI GTMKAC 1 cut(s) 1124
AciI CCGC 1 cut(s) 717
AclWI GGATC 1 cut(s) 2047
AcoI YGGCCR 1 cut(s) 1311
AdeI CACNNNGTG 1 cut(s) 493
AfaI GTAC 6 cut(s) 221, 416, 822, 1318, 1968, 2127
AfiI CCNNNNNNNGG 3 cut(s) 886, 1622, 2070
AflIII ACRYGT 2 cut(s) 499, 825
AjnI CCWGG 2 cut(s) 100, 1219
AjuI GAANNNNNNNTTGG 2 cut(s) 1775, 1807
AloI GAACNNNNNNTCC 2 cut(s) 2021, 2053
Alw21I GWGCWC 5 cut(s) 536, 592, 724, 790, 1018
Alw26I GTCTC 4 cut(s) 111, 625, 1221, 1790
Alw44I GTGCAC 2 cut(s) 588, 1014
AlwI GGATC 1 cut(s) 2047
AoxI GGCC 3 cut(s) 207, 661, 1311
ApaLI GTGCAC 2 cut(s) 588, 1014
ApeKI GCWGC 3 cut(s) 1043, 1612, 2119
Asp700I GAANNNNTTC 2 cut(s) 843, 1937
AsuHPI GGTGA 2 cut(s) 692, 1535
AsuII TTCGAA 2 cut(s) 1258, 1530
BaeGI GKGCMC 3 cut(s) 592, 1018, 2076
BalI TGGCCA 1 cut(s) 1313
BanI GGYRCC 2 cut(s) 198, 2073
BanII GRGCYC 2 cut(s) 322, 790
BarI GAAGNNNNNNTAC 2 cut(s) 831, 863
BauI CACGAG 1 cut(s) 1923
BbsI GAAGAC 2 cut(s) 147, 1341
Bbv12I GWGCWC 5 cut(s) 536, 592, 724, 790, 1018
BbvCI CCTCAGC 1 cut(s) 635
BbvI GCAGC 3 cut(s) 1055, 1624, 2106
BccI CCATC 4 cut(s) 29, 329, 1043, 1670
BciT130I CCWGG 2 cut(s) 102, 1221
BciVI GTATCC 1 cut(s) 1046
BclI TGATCA 1 cut(s) 1540
BcoDI GTCTC 4 cut(s) 111, 625, 1221, 1790
BfaI CTAG 4 cut(s) 624, 1211, 1512, 2093
BfmI CTRYAG 2 cut(s) 1041, 1558
BfuAI ACCTGC 1 cut(s) 301
BfuI GTATCC 1 cut(s) 1046
BisI GCNGC 3 cut(s) 1044, 1613, 2120
BlpI GCTNAGC 1 cut(s) 1608
BlsI GCNGC 3 cut(s) 1045, 1614, 2121
Bme1390I CCNGG 2 cut(s) 102, 1221
BmiI GGNNCC 4 cut(s) 200, 801, 1869, 2075
BmrFI CCNGG 2 cut(s) 102, 1221
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 4 cut(s) 222, 355, 415, 1450
BmuI ACTGGG 1 cut(s) 187
BpiI GAAGAC 2 cut(s) 147, 1341
BplI GAGNNNNNCTC 2 cut(s) 706, 738
BpmI CTGGAG 1 cut(s) 1203
Bpu10I CCTNAGC 1 cut(s) 635
Bpu1102I GCTNAGC 1 cut(s) 1608
Bpu14I TTCGAA 2 cut(s) 1258, 1530
BpuEI CTTGAG 2 cut(s) 1615, 1895
BsaBI GATNNNNATC 1 cut(s) 237
BsaI GGTCTC 1 cut(s) 1790
BsaJI CCNNGG 1 cut(s) 1800
BsaXI ACNNNNNCTCC 4 cut(s) 1287, 1317, 2021, 2051
Bsc4I CCNNNNNNNGG 3 cut(s) 886, 1622, 2070
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 5 cut(s) 193, 1154, 1314, 1436, 1970
Bse3DI GCAATG 3 cut(s) 274, 1137, 2149
Bse8I GATNNNNATC 1 cut(s) 237
BseBI CCWGG 2 cut(s) 102, 1221
BseDI CCNNGG 1 cut(s) 1800
BseGI GGATG 6 cut(s) 40, 52, 517, 544, 1006, 1990
BseJI GATNNNNATC 1 cut(s) 237
BseLI CCNNNNNNNGG 3 cut(s) 886, 1622, 2070
BseMI GCAATG 3 cut(s) 274, 1137, 2149
BseMII CTCAG 3 cut(s) 335, 626, 2143
BseNI ACTGG 5 cut(s) 193, 1154, 1314, 1436, 1970
BseSI GKGCMC 3 cut(s) 592, 1018, 2076
BseXI GCAGC 3 cut(s) 1055, 1624, 2106
BseYI CCCAGC 1 cut(s) 1616
BsgI GTGCAG 1 cut(s) 192
BshFI GGCC 3 cut(s) 209, 663, 1313
BshNI GGYRCC 2 cut(s) 198, 2073
BsiHKAI GWGCWC 5 cut(s) 536, 592, 724, 790, 1018
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 2 cut(s) 510, 906
BslI CCNNNNNNNGG 3 cut(s) 886, 1622, 2070
BsmAI GTCTC 4 cut(s) 111, 625, 1221, 1790
BsmFI GGGAC 2 cut(s) 510, 906
BsmI GAATGC 1 cut(s) 1915
BsnI GGCC 3 cut(s) 209, 663, 1313
Bso31I GGTCTC 1 cut(s) 1790
Bsp119I TTCGAA 2 cut(s) 1258, 1530
Bsp1286I GDGCHC 7 cut(s) 322, 536, 592, 724, 790, 1018, 2076
Bsp143I GATC 6 cut(s) 157, 238, 1084, 1184, 1540, 2052
Bsp1720I GCTNAGC 1 cut(s) 1608
Bsp19I CCATGG 1 cut(s) 1800
BspACI CCGC 1 cut(s) 717
BspANI GGCC 3 cut(s) 209, 663, 1313
BspCNI CTCAG 3 cut(s) 336, 627, 2142
BspHI TCATGA 2 cut(s) 160, 741
BspLI GGNNCC 4 cut(s) 200, 801, 1869, 2075
BspMAI CTGCAG 1 cut(s) 1045
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 1 cut(s) 2047
BspQI GCTCTTC 2 cut(s) 541, 849
BspT104I TTCGAA 2 cut(s) 1258, 1530
BspT107I GGYRCC 2 cut(s) 198, 2073
BspTNI GGTCTC 1 cut(s) 1790
BsrBI CCGCTC 1 cut(s) 717
BsrDI GCAATG 3 cut(s) 274, 1137, 2149
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 5 cut(s) 193, 1154, 1314, 1436, 1970
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 1800
BssMI GATC 6 cut(s) 157, 238, 1084, 1184, 1540, 2052
BssNAI GTATAC 1 cut(s) 1125
BssSI CACGAG 1 cut(s) 1923
BssT1I CCWWGG 1 cut(s) 1800
Bst1107I GTATAC 1 cut(s) 1125
Bst2BI CACGAG 1 cut(s) 1923
Bst2UI CCWGG 2 cut(s) 102, 1221
Bst4CI ACNGT 1 cut(s) 1651
Bst6I CTCTTC 2 cut(s) 541, 849
BstAPI GCANNNNNTGC 1 cut(s) 2080
BstBI TTCGAA 2 cut(s) 1258, 1530
BstC8I GCNNGC 5 cut(s) 98, 211, 1452, 1588, 1915
BstDEI CTNAG 6 cut(s) 344, 635, 651, 1608, 2022, 2129
BstDSI CCRYGG 1 cut(s) 1800
BstF5I GGATG 6 cut(s) 40, 52, 517, 544, 1006, 1990
BstKTI GATC 6 cut(s) 160, 241, 1087, 1187, 1543, 2055
BstMAI GTCTC 4 cut(s) 111, 625, 1221, 1790
BstMBI GATC 6 cut(s) 157, 238, 1084, 1184, 1540, 2052
BstMWI GCNNNNNNNGC 4 cut(s) 1460, 1512, 2080, 2089
BstNI CCWGG 2 cut(s) 102, 1221
BstNSI RCATGY 4 cut(s) 151, 503, 829, 1844
BstSCI CCNGG 2 cut(s) 100, 1219
BstSFI CTRYAG 2 cut(s) 1041, 1558
BstSLI GKGCMC 3 cut(s) 592, 1018, 2076
BstV1I GCAGC 3 cut(s) 1055, 1624, 2106
BstV2I GAAGAC 2 cut(s) 147, 1341
BstXI CCANNNNNNTGG 2 cut(s) 152, 1977
BstZ17I GTATAC 1 cut(s) 1125
BsuI GTATCC 1 cut(s) 1046
BsuRI GGCC 3 cut(s) 209, 663, 1313
BtgI CCRYGG 1 cut(s) 1800
BtsCI GGATG 6 cut(s) 40, 52, 517, 544, 1006, 1990
BtsI GCAGTG 2 cut(s) 1374, 1710
BtsIMutI CAGTG 5 cut(s) 200, 1147, 1374, 1710, 2137
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 5 cut(s) 98, 211, 1452, 1588, 1915
CciI TCATGA 2 cut(s) 160, 741
Cfr10I RCCGGY 1 cut(s) 209
Csp6I GTAC 6 cut(s) 220, 415, 821, 1317, 1967, 2126
CspCI CAANNNNNGTGG 2 cut(s) 1388, 1423
CviQI GTAC 6 cut(s) 220, 415, 821, 1317, 1967, 2126
DdeI CTNAG 6 cut(s) 344, 635, 651, 1608, 2022, 2129
DpnI GATC 6 cut(s) 159, 240, 1086, 1186, 1542, 2054
DpnII GATC 6 cut(s) 157, 238, 1084, 1184, 1540, 2052
DraI TTTAAA 1 cut(s) 1855
DraIII CACNNNGTG 1 cut(s) 493
EaeI YGGCCR 1 cut(s) 1311
Eam1104I CTCTTC 2 cut(s) 541, 849
EarI CTCTTC 2 cut(s) 541, 849
Ecl136II GAGCTC 1 cut(s) 788
Eco130I CCWWGG 1 cut(s) 1800
Eco147I AGGCCT 1 cut(s) 663
Eco24I GRGCYC 2 cut(s) 322, 790
Eco31I GGTCTC 1 cut(s) 1790
Eco53kI GAGCTC 1 cut(s) 788
EcoICRI GAGCTC 1 cut(s) 788
EcoRII CCWGG 2 cut(s) 100, 1219
EcoT14I CCWWGG 1 cut(s) 1800
EcoT22I ATGCAT 1 cut(s) 1465
EcoT38I GRGCYC 2 cut(s) 322, 790
ErhI CCWWGG 1 cut(s) 1800
FaqI GGGAC 2 cut(s) 510, 906
FauNDI CATATG 1 cut(s) 996
FbaI TGATCA 1 cut(s) 1540
FblI GTMKAC 1 cut(s) 1124
Fnu4HI GCNGC 3 cut(s) 1044, 1613, 2120
FokI GGATG 6 cut(s) 47, 59, 524, 531, 993, 1997
FriOI GRGCYC 2 cut(s) 322, 790
Fsp4HI GCNGC 3 cut(s) 1044, 1613, 2120
FspBI CTAG 4 cut(s) 624, 1211, 1512, 2093
GluI GCNGC 3 cut(s) 1044, 1613, 2120
GsaI CCCAGC 1 cut(s) 1620
GsuI CTGGAG 1 cut(s) 1203
HaeIII GGCC 3 cut(s) 209, 663, 1313
HapII CCGG 1 cut(s) 210
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 2 cut(s) 476, 1934
HinfI GANTC 9 cut(s) 130, 181, 260, 919, 1201, 1214, 1501, 1846, 1926
HpaII CCGG 1 cut(s) 210
HphI GGTGA 2 cut(s) 692, 1535
Hpy166II GTNNAC 7 cut(s) 66, 376, 417, 590, 823, 1016, 1125
Hpy188I TCNGA 7 cut(s) 424, 571, 1172, 1189, 1287, 1581, 1773
Hpy188III TCNNGA 8 cut(s) 161, 742, 1211, 1229, 1520, 1666, 1923, 1930
Hpy8I GTNNAC 7 cut(s) 66, 376, 417, 590, 823, 1016, 1125
HpyAV CCTTC 3 cut(s) 578, 934, 1620
HpyCH4III ACNGT 1 cut(s) 1651
HpyCH4IV ACGT 1 cut(s) 530
HpyF10VI GCNNNNNNNGC 4 cut(s) 1460, 1512, 2080, 2089
HpyF3I CTNAG 6 cut(s) 344, 635, 651, 1608, 2022, 2129
HpySE526I ACGT 1 cut(s) 530
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 1540
Kzo9I GATC 6 cut(s) 157, 238, 1084, 1184, 1540, 2052
LguI GCTCTTC 2 cut(s) 541, 849
LmnI GCTCC 2 cut(s) 785, 1867
Lsp1109I GCAGC 3 cut(s) 1055, 1624, 2106
LweI GCATC 4 cut(s) 222, 355, 415, 1450
MaeI CTAG 4 cut(s) 624, 1211, 1512, 2093
MaeII ACGT 1 cut(s) 530
MaeIII GTNAC 4 cut(s) 1036, 1645, 1690, 2017
MalI GATC 6 cut(s) 159, 240, 1086, 1186, 1542, 2054
MbiI CCGCTC 1 cut(s) 717
MboI GATC 6 cut(s) 157, 238, 1084, 1184, 1540, 2052
MboII GAAGA 7 cut(s) 85, 152, 528, 699, 836, 1341, 1646
MfeI CAATTG 2 cut(s) 774, 1061
MhlI GDGCHC 7 cut(s) 322, 536, 592, 724, 790, 1018, 2076
MlsI TGGCCA 1 cut(s) 1313
MluNI TGGCCA 1 cut(s) 1313
MlyI GAGTC 4 cut(s) 175, 928, 1223, 1935
MmeI TCCRAC 2 cut(s) 763, 1786
Mox20I TGGCCA 1 cut(s) 1313
Mph1103I ATGCAT 1 cut(s) 1465
MroNI GCCGGC 1 cut(s) 209
MroXI GAANNNNTTC 2 cut(s) 843, 1937
MscI TGGCCA 1 cut(s) 1313
MseI TTAA 8 cut(s) 78, 84, 690, 987, 1367, 1484, 1854, 2010
MslI CAYNNNNRTG 3 cut(s) 510, 999, 1011
Msp20I TGGCCA 1 cut(s) 1313
MspA1I CMGCKG 1 cut(s) 1046
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 102, 1221
MunI CAATTG 2 cut(s) 774, 1061
Mva1269I GAATGC 1 cut(s) 1915
MvaI CCWGG 2 cut(s) 102, 1221
MwoI GCNNNNNNNGC 4 cut(s) 1460, 1512, 2080, 2089
NaeI GCCGGC 1 cut(s) 211
NcoI CCATGG 1 cut(s) 1800
NdeI CATATG 1 cut(s) 996
NdeII GATC 6 cut(s) 157, 238, 1084, 1184, 1540, 2052
NgoMIV GCCGGC 1 cut(s) 209
NlaIV GGNNCC 4 cut(s) 200, 801, 1869, 2075
NmuCI GTSAC 2 cut(s) 1645, 1690
NsiI ATGCAT 1 cut(s) 1465
NspI RCATGY 4 cut(s) 151, 503, 829, 1844
NspV TTCGAA 2 cut(s) 1258, 1530
PagI TCATGA 2 cut(s) 160, 741
PaqCI CACCTGC 1 cut(s) 301
PceI AGGCCT 1 cut(s) 663
PciI ACATGT 2 cut(s) 499, 825
PciSI GCTCTTC 2 cut(s) 541, 849
PctI GAATGC 1 cut(s) 1915
PdiI GCCGGC 1 cut(s) 211
PdmI GAANNNNTTC 2 cut(s) 843, 1937
PfeI GAWTC 5 cut(s) 130, 260, 1201, 1501, 1846
PflMI CCANNNNNTGG 1 cut(s) 886
PfoI TCCNGGA 1 cut(s) 1219
PkrI GCNGC 3 cut(s) 1045, 1614, 2121
PleI GAGTC 4 cut(s) 175, 927, 1222, 1934
PpsI GAGTC 4 cut(s) 175, 927, 1222, 1934
PscI ACATGT 2 cut(s) 499, 825
PsiI TTATAA 2 cut(s) 951, 1071
Psp124BI GAGCTC 1 cut(s) 790
Psp6I CCWGG 2 cut(s) 100, 1219
PspFI CCCAGC 1 cut(s) 1616
PspGI CCWGG 2 cut(s) 100, 1219
PspN4I GGNNCC 4 cut(s) 200, 801, 1869, 2075
PstI CTGCAG 1 cut(s) 1045
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 6 cut(s) 221, 416, 822, 1318, 1968, 2127
RsaNI GTAC 6 cut(s) 220, 415, 821, 1317, 1967, 2126
RseI CAYNNNNRTG 3 cut(s) 510, 999, 1011
SacI GAGCTC 1 cut(s) 790
SapI GCTCTTC 2 cut(s) 541, 849
SaqAI TTAA 8 cut(s) 78, 84, 690, 987, 1367, 1484, 1854, 2010
SatI GCNGC 3 cut(s) 1044, 1613, 2120
Sau3AI GATC 6 cut(s) 157, 238, 1084, 1184, 1540, 2052
SchI GAGTC 4 cut(s) 175, 928, 1223, 1935
ScrFI CCNGG 2 cut(s) 102, 1221
SduI GDGCHC 7 cut(s) 322, 536, 592, 724, 790, 1018, 2076
SfaNI GCATC 4 cut(s) 222, 355, 415, 1450
SfcI CTRYAG 2 cut(s) 1041, 1558
SfuI TTCGAA 2 cut(s) 1258, 1530
SmiMI CAYNNNNRTG 3 cut(s) 510, 999, 1011
SmlI CTYRAG 2 cut(s) 1594, 1874
SmoI CTYRAG 2 cut(s) 1594, 1874
SseBI AGGCCT 1 cut(s) 663
SsiI CCGC 1 cut(s) 717
SspMI CTAG 4 cut(s) 624, 1211, 1512, 2093
SstI GAGCTC 1 cut(s) 790
StuI AGGCCT 1 cut(s) 663
StyD4I CCNGG 2 cut(s) 100, 1219
StyI CCWWGG 1 cut(s) 1800
TaaI ACNGT 1 cut(s) 1651
TaiI ACGT 1 cut(s) 533
TaqI TCGA 2 cut(s) 1258, 1530
TatI WGTACW 3 cut(s) 219, 820, 1316
TfiI GAWTC 5 cut(s) 130, 260, 1201, 1501, 1846
Tru1I TTAA 8 cut(s) 78, 84, 690, 987, 1367, 1484, 1854, 2010
Tru9I TTAA 8 cut(s) 78, 84, 690, 987, 1367, 1484, 1854, 2010
TscAI CASTG 5 cut(s) 200, 1154, 1381, 1717, 2137
TseFI GTSAC 2 cut(s) 1645, 1690
TseI GCWGC 3 cut(s) 1043, 1612, 2119
Tsp45I GTSAC 2 cut(s) 1645, 1690
TspRI CASTG 5 cut(s) 200, 1154, 1381, 1717, 2137
Van91I CCANNNNNTGG 1 cut(s) 886
VneI GTGCAC 2 cut(s) 588, 1014
XbaI TCTAGA 1 cut(s) 1210
XceI RCATGY 4 cut(s) 151, 503, 829, 1844
XmiI GTMKAC 1 cut(s) 1124
XmnI GAANNNNTTC 2 cut(s) 843, 1937
XspI CTAG 4 cut(s) 624, 1211, 1512, 2093
Zsp2I ATGCAT 1 cut(s) 1465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.