Rmu_sc0006153.1_g000015

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006153.1
OG0005168
Physical Location & Seq
Forward (+)
62010 .. 63668
1659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006153.1_g000015.1.cds

Sequence Viewer

Length: 1539 bp
atgttcaattttcatcactggctttgcattgttcttgtccgagcatttgcaagatcagaagtcttgctgaatcatgttctagagtctccaggacttcacgaatggttggcaagagctattgaaattagaaatcctaatgctttgattttggctctgaaaatgggagaaaaagtttatgtggccagtacaggatttgggaaacttcttgctaatagtttaatggacactgctttctgtcagccacgcagccacaatgtttggcctgttcaattacacattttattaccagatagatttttgcaggctgaagatgcattgaccatttcaaatcccttaacgaagcacaaaaggaatcgcaagtatagctctcctgatgatttcgaagtttatgatcacctatttgatttctacagaattttcattgaacctctgattgcttactacttgagatacatagctaagcagcccagctatggaaggtatgaagatagtcctgtgacagtttcatctaccatcgtgaatggagaaaaatgtatgtttgtgactattgacaaattatgcattgctttagttttcaagtgttgtctaattggtagagttgaggtgccagagaagtggtttctgaatgtgatgaatatgaatttggagaccatagttggaaaagttagatgtggtagggaaaaggagttgcatgtgaatctcttagcggatccataccccctaccagcacagcccaccaccaagccgccacatctgatcgacggcgccgtttcggctcagccgaacaatattggggagggagagaattctgataagacggttaaggtgcatagtggggttggtagagagttcaatgagctagagagtgttactgttaagcaagatgatcagggaccaagtttgtgtaggaaaagaagagaataccatgcactctacatgtttgataaaatgttgcaaccagatgggatgtgtaatggaggaaggactattatagctcctatgagcattcgagacaaagggggaggactcactacccatcgtgaagttgttgatgtttctgatgagatggcgatatgttctgtcagtgctaagcaggacgattatggtttcgttgaagataaggggcaggtagacttcaaaactcctttaatatctaactctctgaatacagaaaatgatgaagttgtgaatgccaagattgaagtagtggaaaccctttatgaacattctgatgagaaattagatattggggagggagagagttctaataagttgacagagagttcaaagggaaatttggatttggatgggctttgggatgatagtggtggattgtccaccatgaagattaaatttaagagtttctgcaagcctagtttggtgcttggagactctgagctgattcatatagaagaccacatgcttagagatcatgagcaaagtgcagttatgaatcactattcccagtggtgccaaaaggccggcatcaagtacaaaagttgtgatgatgatcttgttggtgttgtggaggattcataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

512

Amino Acids

57.75

Weight (kDa)

5.49

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1117
AccB1I GGYRCC 3 cut(s) 604, 764, 1470
AccI GTMKAC 1 cut(s) 1131
AciI CCGC 2 cut(s) 707, 746
AclWI GGATC 2 cut(s) 704, 717
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 5 cut(s) 414, 640, 805, 1294, 1352
AcuI CTGAAG 1 cut(s) 327
AcyI GRCGYC 1 cut(s) 765
AfaI GTAC 2 cut(s) 187, 1493
AfiI CCNNNNNNNGG 1 cut(s) 473
AflIII ACRYGT 1 cut(s) 936
AjnI CCWGG 1 cut(s) 88
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AluBI AGCT 7 cut(s) 116, 366, 458, 471, 859, 995, 1399
AluI AGCT 7 cut(s) 116, 366, 458, 471, 859, 995, 1399
Alw26I GTCTC 4 cut(s) 90, 641, 1005, 1383
AlwI GGATC 2 cut(s) 704, 717
AoxI GGCC 3 cut(s) 180, 260, 1479
ApeKI GCWGC 2 cut(s) 246, 463
ApoI RAATTY 5 cut(s) 414, 640, 805, 1294, 1352
AspLEI GCGC 1 cut(s) 767
AspS9I GGNCC 1 cut(s) 893
AsuHPI GGTGA 1 cut(s) 386
AsuII TTCGAA 1 cut(s) 381
AvaII GGWCC 1 cut(s) 893
BalI TGGCCA 1 cut(s) 182
BamHI GGATCC 1 cut(s) 709
BanI GGYRCC 3 cut(s) 604, 764, 1470
BbsI GAAGAC 1 cut(s) 1419
BbvI GCAGC 2 cut(s) 258, 475
BccI CCATC 5 cut(s) 521, 956, 1044, 1060, 1301
BceAI ACGGC 2 cut(s) 752, 778
BciT130I CCWGG 1 cut(s) 90
BclI TGATCA 2 cut(s) 391, 886
BcoDI GTCTC 4 cut(s) 90, 641, 1005, 1383
BfaI CTAG 3 cut(s) 80, 860, 1374
BfmI CTRYAG 1 cut(s) 409
BfoI RGCGCY 1 cut(s) 768
BfuAI ACCTGC 1 cut(s) 1117
BglI GCCNNNNNGGC 1 cut(s) 773
BisI GCNGC 3 cut(s) 247, 464, 746
BlpI GCTNAGC 3 cut(s) 459, 777, 1089
BlsI GCNGC 3 cut(s) 248, 465, 747
Bme1390I CCNGG 1 cut(s) 90
Bme18I GGWCC 1 cut(s) 893
BmgT120I GGNCC 1 cut(s) 893
BmiI GGNNCC 5 cut(s) 606, 711, 766, 894, 1472
BmrFI CCNGG 1 cut(s) 90
BmrI ACTGGG 1 cut(s) 1459
BmsI GCATC 2 cut(s) 301, 1494
BmuI ACTGGG 1 cut(s) 1459
BpiI GAAGAC 1 cut(s) 1419
BpmI CTGGAG 1 cut(s) 72
Bpu1102I GCTNAGC 3 cut(s) 459, 777, 1089
Bpu14I TTCGAA 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 466
BsaBI GATNNNNATC 1 cut(s) 1509
BsaHI GRCGYC 1 cut(s) 765
BsaI GGTCTC 1 cut(s) 641
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse118I RCCGGY 1 cut(s) 1481
Bse1I ACTGG 3 cut(s) 23, 183, 1465
Bse3DI GCAATG 1 cut(s) 561
Bse8I GATNNNNATC 1 cut(s) 1509
BseBI CCWGG 1 cut(s) 90
BseGI GGATG 3 cut(s) 972, 1312, 1324
BseJI GATNNNNATC 1 cut(s) 1509
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 2 cut(s) 791, 1386
BseNI ACTGG 3 cut(s) 23, 183, 1465
BseXI GCAGC 2 cut(s) 258, 475
BseYI CCCAGC 1 cut(s) 467
BsgI GTGCAG 1 cut(s) 1464
BshFI GGCC 3 cut(s) 182, 262, 1481
BshNI GGYRCC 3 cut(s) 604, 764, 1470
BsiSI CCGG 1 cut(s) 1482
BslFI GGGAC 1 cut(s) 906
BslI CCNNNNNNNGG 1 cut(s) 473
BsmAI GTCTC 4 cut(s) 90, 641, 1005, 1383
BsmFI GGGAC 1 cut(s) 906
BsmI GAATGC 2 cut(s) 1005, 1195
BsnI GGCC 3 cut(s) 182, 262, 1481
Bso31I GGTCTC 1 cut(s) 641
Bsp119I TTCGAA 1 cut(s) 381
Bsp143I GATC 7 cut(s) 53, 391, 709, 756, 886, 1429, 1510
Bsp1720I GCTNAGC 3 cut(s) 459, 777, 1089
BspACI CCGC 2 cut(s) 707, 746
BspANI GGCC 3 cut(s) 182, 262, 1481
BspCNI CTCAG 2 cut(s) 790, 1387
BspHI TCATGA 1 cut(s) 1432
BspLI GGNNCC 5 cut(s) 606, 711, 766, 894, 1472
BspMI ACCTGC 1 cut(s) 1117
BspPI GGATC 2 cut(s) 704, 717
BspT104I TTCGAA 1 cut(s) 381
BspT107I GGYRCC 3 cut(s) 604, 764, 1470
BspTNI GGTCTC 1 cut(s) 641
BsrDI GCAATG 1 cut(s) 561
BsrFI RCCGGY 1 cut(s) 1481
BsrI ACTGG 3 cut(s) 23, 183, 1465
BssAI RCCGGY 1 cut(s) 1481
BssMI GATC 7 cut(s) 53, 391, 709, 756, 886, 1429, 1510
BssNI GRCGYC 1 cut(s) 765
Bst2UI CCWGG 1 cut(s) 90
Bst4CI ACNGT 3 cut(s) 502, 820, 874
Bst6I CTCTTC 1 cut(s) 910
BstACI GRCGYC 1 cut(s) 765
BstBI TTCGAA 1 cut(s) 381
BstC8I GCNNGC 3 cut(s) 303, 1370, 1483
BstDEI CTNAG 6 cut(s) 459, 703, 777, 1089, 1395, 1424
BstF5I GGATG 3 cut(s) 972, 1312, 1324
BstH2I RGCGCY 1 cut(s) 768
BstHHI GCGC 1 cut(s) 767
BstKTI GATC 7 cut(s) 56, 394, 712, 759, 889, 1432, 1513
BstMAI GTCTC 4 cut(s) 90, 641, 1005, 1383
BstMBI GATC 7 cut(s) 53, 391, 709, 756, 886, 1429, 1510
BstMWI GCNNNNNNNGC 3 cut(s) 311, 363, 773
BstNI CCWGG 1 cut(s) 90
BstNSI RCATGY 3 cut(s) 695, 940, 1423
BstSCI CCNGG 1 cut(s) 88
BstSFI CTRYAG 1 cut(s) 409
BstV1I GCAGC 2 cut(s) 258, 475
BstV2I GAAGAC 1 cut(s) 1419
BstX2I RGATCY 1 cut(s) 709
BstXI CCANNNNNNTGG 1 cut(s) 615
BstYI RGATCY 1 cut(s) 709
BsuRI GGCC 3 cut(s) 182, 262, 1481
BtsCI GGATG 3 cut(s) 972, 1312, 1324
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 4 cut(s) 16, 225, 1090, 1472
BveI ACCTGC 1 cut(s) 1117
Cac8I GCNNGC 3 cut(s) 303, 1370, 1483
CciI TCATGA 1 cut(s) 1432
CfoI GCGC 1 cut(s) 767
Cfr10I RCCGGY 1 cut(s) 1481
Cfr13I GGNCC 1 cut(s) 893
Csp6I GTAC 2 cut(s) 186, 1492
CspCI CAANNNNNGTGG 2 cut(s) 239, 274
CviAII CATG 7 cut(s) 74, 692, 926, 937, 1342, 1420, 1433
CviQI GTAC 2 cut(s) 186, 1492
DdeI CTNAG 6 cut(s) 459, 703, 777, 1089, 1395, 1424
DinI GGCGCC 1 cut(s) 766
DpnI GATC 7 cut(s) 55, 393, 711, 758, 888, 1431, 1512
DpnII GATC 7 cut(s) 53, 391, 709, 756, 886, 1429, 1510
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 910
EarI CTCTTC 1 cut(s) 910
Eco31I GGTCTC 1 cut(s) 641
Eco47I GGWCC 1 cut(s) 893
Eco57I CTGAAG 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 805
EcoRII CCWGG 1 cut(s) 88
EcoT22I ATGCAT 2 cut(s) 316, 563
EgeI GGCGCC 1 cut(s) 766
EheI GGCGCC 1 cut(s) 766
FaeI CATG 7 cut(s) 77, 695, 929, 940, 1345, 1423, 1436
FaqI GGGAC 1 cut(s) 906
FatI CATG 7 cut(s) 73, 691, 925, 936, 1341, 1419, 1432
FbaI TGATCA 2 cut(s) 391, 886
FblI GTMKAC 1 cut(s) 1131
Fnu4HI GCNGC 3 cut(s) 247, 464, 746
FokI GGATG 3 cut(s) 979, 1319, 1331
Fsp4HI GCNGC 3 cut(s) 247, 464, 746
FspBI CTAG 3 cut(s) 80, 860, 1374
GlaI GCGC 1 cut(s) 766
GluI GCNGC 3 cut(s) 247, 464, 746
GsaI CCCAGC 1 cut(s) 471
GsuI CTGGAG 1 cut(s) 72
HaeII RGCGCY 1 cut(s) 768
HaeIII GGCC 3 cut(s) 182, 262, 1481
HapII CCGG 1 cut(s) 1482
HhaI GCGC 1 cut(s) 767
Hin1I GRCGYC 1 cut(s) 765
Hin1II CATG 7 cut(s) 77, 695, 929, 940, 1345, 1423, 1436
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HincII GTYRAC 1 cut(s) 1275
HindII GTYRAC 1 cut(s) 1275
HinfI GANTC 9 cut(s) 70, 83, 352, 697, 1026, 1391, 1402, 1453, 1532
HpaII CCGG 1 cut(s) 1482
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 3 cut(s) 1132, 1275, 1338
Hpy188III TCNNGA 7 cut(s) 80, 98, 371, 517, 1010, 1040, 1433
Hpy8I GTNNAC 3 cut(s) 1132, 1275, 1338
Hpy99I CGWCG 1 cut(s) 764
HpyAV CCTTC 2 cut(s) 471, 975
HpyCH4III ACNGT 3 cut(s) 502, 820, 874
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 363, 773
HpyF3I CTNAG 6 cut(s) 459, 703, 777, 1089, 1395, 1424
Hsp92I GRCGYC 1 cut(s) 765
Hsp92II CATG 7 cut(s) 77, 695, 929, 940, 1345, 1423, 1436
HspAI GCGC 1 cut(s) 765
KasI GGCGCC 1 cut(s) 764
KroI GCCGGC 1 cut(s) 1481
KroNI GCCGGC 1 cut(s) 1483
Ksp22I TGATCA 2 cut(s) 391, 886
Kzo9I GATC 7 cut(s) 53, 391, 709, 756, 886, 1429, 1510
LmnI GCTCC 1 cut(s) 1000
Lsp1109I GCAGC 2 cut(s) 258, 475
LweI GCATC 2 cut(s) 301, 1494
MaeI CTAG 3 cut(s) 80, 860, 1374
MaeIII GTNAC 3 cut(s) 496, 541, 868
MalI GATC 7 cut(s) 55, 393, 711, 758, 888, 1431, 1512
MboI GATC 7 cut(s) 53, 391, 709, 756, 886, 1429, 1510
MboII GAAGA 6 cut(s) 320, 497, 927, 1127, 1357, 1424
MflI RGATCY 1 cut(s) 709
MlsI TGGCCA 1 cut(s) 182
MluNI TGGCCA 1 cut(s) 182
Mly113I GGCGCC 1 cut(s) 765
MlyI GAGTC 3 cut(s) 92, 1020, 1385
MmeI TCCRAC 1 cut(s) 637
MnlI CCTC 7 cut(s) 438, 595, 790, 971, 1016, 1246, 1522
Mox20I TGGCCA 1 cut(s) 182
Mph1103I ATGCAT 2 cut(s) 316, 563
MroNI GCCGGC 1 cut(s) 1481
MscI TGGCCA 1 cut(s) 182
MseI TTAA 7 cut(s) 218, 335, 822, 876, 1148, 1350, 1356
MslI CAYNNNNRTG 1 cut(s) 1230
Msp20I TGGCCA 1 cut(s) 182
MspI CCGG 1 cut(s) 1482
MspR9I CCNGG 1 cut(s) 90
Mva1269I GAATGC 2 cut(s) 1005, 1195
MvaI CCWGG 1 cut(s) 90
MwoI GCNNNNNNNGC 3 cut(s) 311, 363, 773
NaeI GCCGGC 1 cut(s) 1483
NarI GGCGCC 1 cut(s) 765
NdeII GATC 7 cut(s) 53, 391, 709, 756, 886, 1429, 1510
NgoMIV GCCGGC 1 cut(s) 1481
NlaIII CATG 7 cut(s) 77, 695, 929, 940, 1345, 1423, 1436
NlaIV GGNNCC 5 cut(s) 606, 711, 766, 894, 1472
NmuCI GTSAC 2 cut(s) 496, 541
NsiI ATGCAT 2 cut(s) 316, 563
NspI RCATGY 3 cut(s) 695, 940, 1423
NspV TTCGAA 1 cut(s) 381
PagI TCATGA 1 cut(s) 1432
PciI ACATGT 1 cut(s) 936
PcsI WCGNNNNNNNCGW 2 cut(s) 765, 779
PctI GAATGC 2 cut(s) 1005, 1195
PdiI GCCGGC 1 cut(s) 1483
PfeI GAWTC 6 cut(s) 70, 352, 697, 1402, 1453, 1532
PfoI TCCNGGA 1 cut(s) 88
PkrI GCNGC 3 cut(s) 248, 465, 747
PleI GAGTC 3 cut(s) 91, 1020, 1385
PluTI GGCGCC 1 cut(s) 768
PpsI GAGTC 3 cut(s) 91, 1020, 1385
PscI ACATGT 1 cut(s) 936
Psp6I CCWGG 1 cut(s) 88
PspFI CCCAGC 1 cut(s) 467
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 5 cut(s) 606, 711, 766, 894, 1472
PspPI GGNCC 1 cut(s) 893
PsuI RGATCY 1 cut(s) 709
RsaI GTAC 2 cut(s) 187, 1493
RsaNI GTAC 2 cut(s) 186, 1492
RseI CAYNNNNRTG 1 cut(s) 1230
SaqAI TTAA 7 cut(s) 218, 335, 822, 876, 1148, 1350, 1356
SatI GCNGC 3 cut(s) 247, 464, 746
Sau3AI GATC 7 cut(s) 53, 391, 709, 756, 886, 1429, 1510
Sau96I GGNCC 1 cut(s) 893
SchI GAGTC 3 cut(s) 92, 1020, 1385
ScrFI CCNGG 1 cut(s) 90
SfaNI GCATC 2 cut(s) 301, 1494
SfcI CTRYAG 1 cut(s) 409
SfoI GGCGCC 1 cut(s) 766
SfuI TTCGAA 1 cut(s) 381
SinI GGWCC 1 cut(s) 893
SmiMI CAYNNNNRTG 1 cut(s) 1230
SmlI CTYRAG 1 cut(s) 445
SmoI CTYRAG 1 cut(s) 445
SsiI CCGC 2 cut(s) 707, 746
SspDI GGCGCC 1 cut(s) 764
SspI AATATT 1 cut(s) 790
SspMI CTAG 3 cut(s) 80, 860, 1374
StyD4I CCNGG 1 cut(s) 88
TaaI ACNGT 3 cut(s) 502, 820, 874
TaqI TCGA 3 cut(s) 381, 759, 1009
TatI WGTACW 2 cut(s) 185, 1491
TauI GCSGC 1 cut(s) 748
TfiI GAWTC 6 cut(s) 70, 352, 697, 1402, 1453, 1532
Tru1I TTAA 7 cut(s) 218, 335, 822, 876, 1148, 1350, 1356
Tru9I TTAA 7 cut(s) 218, 335, 822, 876, 1148, 1350, 1356
TscAI CASTG 4 cut(s) 23, 232, 1090, 1472
TseFI GTSAC 2 cut(s) 496, 541
TseI GCWGC 2 cut(s) 246, 463
Tsp45I GTSAC 2 cut(s) 496, 541
TspRI CASTG 4 cut(s) 23, 232, 1090, 1472
VpaK11BI GGWCC 1 cut(s) 893
XapI RAATTY 5 cut(s) 414, 640, 805, 1294, 1352
XbaI TCTAGA 1 cut(s) 79
XceI RCATGY 3 cut(s) 695, 940, 1423
XmiI GTMKAC 1 cut(s) 1131
XspI CTAG 3 cut(s) 80, 860, 1374
Zsp2I ATGCAT 2 cut(s) 316, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.