FvH4_1g27280

ALA-interacting subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001483
Physical Location & Seq
Forward (+)
19002483 .. 19006196
3714 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27280.t1

Sequence Viewer

Length: 1053 bp
ATGGCTACGAAAGCTGAAAAGGGCAAAGATGGAGGAGACTCGTCCTTGGCCAAAAAGAAGTCCAGAAGACCCCAATATTCCAGGTTCTCACAGCAAGAGCTTCCGGCTTGTAAACCAATTTTGTCACCAGGATGGGTGGTTTCAATATTTTTCACCATTGGCATTGTCTTCATCCCTATTGGTTTTGCTGCCTTATTTGCCTCGGAGCGTGTGGTGGAGATAAAGTTCCAATATGACCAAGACTGTGTTCCTGCAAAGTATAAGAATGATATGGTTGCGTATATTCAAAGCAATGACACTGACAAGACCTGTACGAGGAAAATGCCTGCGGTTTCGGCTACAATGAAGTCTCCGATTTATGTCTACTATCAGATTGATCATTACTTTCAGAATCATCGTCGATATGTTAAAAGTAGAAATGACGAACAGTTGCGGGGAGATCAAAATAATACAGCCGACTGTGCCCCTGAACGCTTTGCAAACAATGGTCAAGCAATTGTTCCTTGTGGCCTCGTTGCATGGAGTTTGTTTAATGACACATATAAGTTTTCAGTGAAAAACAAAACAGTAGAAGTCAGCAAAAAGGACATCACATGGAAAAGTGACCGTGAGAAAAAGTTTTCAAGCAAAGTCTATCCCAAGAACTTTCAGGCTGGGGGTTTGATTGGAGGAGGGAAGCTCAATTCTAGCATACCTCTGAGCGGACAAGAGGATCTTATTGTCTGGATGCGAACTGCAGCACTGCCAAATTTTAGAAAATTGTATGGACGGATAGAAGAGGACCTTGAAGCTCATGACCAAATAACAATAGAACTACAGAATAACTATAATACCTACAGCTTTAAAGGCACCAAGTCGGTGATACTTTCTACTGCGAGTTGGATTGGTGGAAAGAATGATCTAATGGGCATAGCATACCTTACTATTGGTGGAACATGTTTGTTCTTGGCAATATGCTTCATACTTATGTATGTGCTTAAGCCGAGACCTCTTGGGGATACCTCATACTTGTCTTGGAATAGAAATGCATCAGGAGGAGGCCTACGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC50

Protein Analysis

351

Amino Acids

39.4

Weight (kDa)

9.38

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC50 PF03381 65 - 329 4.8e-86 LEM3 (ligand-effect modulator 3) family / CDC50 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 848
AccBSI CCGCTC 1 cut(s) 702
AccI GTMKAC 1 cut(s) 363
AciI CCGC 3 cut(s) 329, 433, 702
AclWI GGATC 1 cut(s) 720
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 748
AfaI GTAC 1 cut(s) 313
AfiI CCNNNNNNNGG 2 cut(s) 315, 701
AflII CTTAAG 1 cut(s) 977
AflIII ACRYGT 1 cut(s) 935
AgsI TTSAA 4 cut(s) 144, 287, 624, 788
AjnI CCWGG 2 cut(s) 80, 127
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
AluBI AGCT 5 cut(s) 14, 100, 679, 791, 840
AluI AGCT 5 cut(s) 14, 100, 679, 791, 840
Alw26I GTCTC 3 cut(s) 30, 354, 979
AlwI GGATC 1 cut(s) 720
AoxI GGCC 3 cut(s) 48, 508, 1039
ApeKI GCWGC 2 cut(s) 188, 737
ApoI RAATTY 1 cut(s) 748
AspS9I GGNCC 1 cut(s) 781
AsuHPI GGTGA 3 cut(s) 117, 145, 871
AvaII GGWCC 1 cut(s) 781
BaeGI GKGCMC 1 cut(s) 466
BalI TGGCCA 1 cut(s) 50
BanI GGYRCC 1 cut(s) 848
BbsI GAAGAC 2 cut(s) 73, 160
BbvI GCAGC 2 cut(s) 175, 749
BccI CCATC 2 cut(s) 23, 126
BcgI CGANNNNNNTGC 2 cut(s) 304, 338
BciT130I CCWGG 2 cut(s) 82, 129
BciVI GTATCC 1 cut(s) 991
BclI TGATCA 1 cut(s) 376
BcoDI GTCTC 3 cut(s) 30, 354, 979
BfaI CTAG 1 cut(s) 687
BfmI CTRYAG 3 cut(s) 735, 815, 835
BfrI CTTAAG 1 cut(s) 977
BfuI GTATCC 1 cut(s) 991
BisI GCNGC 2 cut(s) 189, 738
BlsI GCNGC 2 cut(s) 190, 739
Bme1390I CCNGG 2 cut(s) 82, 129
Bme18I GGWCC 1 cut(s) 781
BmgT120I GGNCC 1 cut(s) 781
BmiI GGNNCC 1 cut(s) 850
BmrFI CCNGG 2 cut(s) 82, 129
BmsI GCATC 2 cut(s) 717, 1037
BpiI GAAGAC 2 cut(s) 73, 160
BplI GAGNNNNNCTC 2 cut(s) 663, 695
BsaI GGTCTC 1 cut(s) 979
BsaJI CCNNGG 2 cut(s) 45, 201
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 2 cut(s) 315, 701
Bse3DI GCAATG 1 cut(s) 298
BseBI CCWGG 2 cut(s) 82, 129
BseDI CCNNGG 2 cut(s) 45, 201
BseGI GGATG 3 cut(s) 137, 171, 732
BseLI CCNNNNNNNGG 2 cut(s) 315, 701
BseMI GCAATG 1 cut(s) 298
BseMII CTCAG 1 cut(s) 689
BseRI GAGGAG 3 cut(s) 48, 684, 1050
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 2 cut(s) 175, 749
BseYI CCCAGC 1 cut(s) 653
BshFI GGCC 3 cut(s) 50, 510, 1041
BshNI GGYRCC 1 cut(s) 848
BsiSI CCGG 1 cut(s) 104
BslI CCNNNNNNNGG 2 cut(s) 315, 701
BsmAI GTCTC 3 cut(s) 30, 354, 979
BsnI GGCC 3 cut(s) 50, 510, 1041
Bso31I GGTCTC 1 cut(s) 979
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 4 cut(s) 376, 439, 712, 898
BspACI CCGC 3 cut(s) 329, 433, 702
BspANI GGCC 3 cut(s) 50, 510, 1041
BspCNI CTCAG 1 cut(s) 690
BspHI TCATGA 1 cut(s) 793
BspLI GGNNCC 1 cut(s) 850
BspMAI CTGCAG 1 cut(s) 739
BspPI GGATC 1 cut(s) 720
BspT107I GGYRCC 1 cut(s) 848
BspTI CTTAAG 1 cut(s) 977
BspTNI GGTCTC 1 cut(s) 979
BsrBI CCGCTC 1 cut(s) 702
BsrDI GCAATG 1 cut(s) 298
BssECI CCNNGG 2 cut(s) 45, 201
BssMI GATC 4 cut(s) 376, 439, 712, 898
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 2 cut(s) 82, 129
Bst4CI ACNGT 5 cut(s) 245, 429, 461, 568, 608
Bst6I CTCTTC 1 cut(s) 771
BstAFI CTTAAG 1 cut(s) 977
BstC8I GCNNGC 1 cut(s) 327
BstDEI CTNAG 1 cut(s) 698
BstENI CCTNNNNNAGG 1 cut(s) 313
BstF5I GGATG 3 cut(s) 137, 171, 732
BstKTI GATC 4 cut(s) 379, 442, 715, 901
BstMAI GTCTC 3 cut(s) 30, 354, 979
BstMBI GATC 4 cut(s) 376, 439, 712, 898
BstMWI GCNNNNNNNGC 5 cut(s) 11, 197, 335, 461, 846
BstNI CCWGG 2 cut(s) 82, 129
BstNSI RCATGY 1 cut(s) 939
BstSCI CCNGG 2 cut(s) 80, 127
BstSFI CTRYAG 3 cut(s) 735, 815, 835
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 2 cut(s) 175, 749
BstV2I GAAGAC 2 cut(s) 73, 160
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
BsuI GTATCC 1 cut(s) 991
BsuRI GGCC 3 cut(s) 50, 510, 1041
BtsCI GGATG 3 cut(s) 137, 171, 732
BtsI GCAGTG 1 cut(s) 740
BtsIMutI CAGTG 3 cut(s) 297, 558, 740
Cac8I GCNNGC 1 cut(s) 327
CciI TCATGA 1 cut(s) 793
Cfr13I GGNCC 1 cut(s) 781
Csp6I GTAC 1 cut(s) 312
CviAII CATG 4 cut(s) 519, 594, 794, 936
CviQI GTAC 1 cut(s) 312
DdeI CTNAG 1 cut(s) 698
DpnI GATC 4 cut(s) 378, 441, 714, 900
DpnII GATC 4 cut(s) 376, 439, 712, 898
DraI TTTAAA 1 cut(s) 844
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 771
EarI CTCTTC 1 cut(s) 771
Eco130I CCWWGG 1 cut(s) 45
Eco147I AGGCCT 1 cut(s) 1041
Eco31I GGTCTC 1 cut(s) 979
Eco47I GGWCC 1 cut(s) 781
EcoNI CCTNNNNNAGG 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 781
EcoRII CCWGG 2 cut(s) 80, 127
EcoT14I CCWWGG 1 cut(s) 45
EcoT22I ATGCAT 1 cut(s) 1030
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 4 cut(s) 522, 597, 797, 939
FalI AAGNNNNNCTT 4 cut(s) 699, 731, 768, 800
FatI CATG 4 cut(s) 518, 593, 793, 935
FauI CCCGC 1 cut(s) 426
FbaI TGATCA 1 cut(s) 376
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 2 cut(s) 189, 738
FokI GGATG 3 cut(s) 144, 158, 739
Fsp4HI GCNGC 2 cut(s) 189, 738
FspBI CTAG 1 cut(s) 687
GluI GCNGC 2 cut(s) 189, 738
GsaI CCCAGC 1 cut(s) 657
HaeIII GGCC 3 cut(s) 50, 510, 1041
HapII CCGG 1 cut(s) 104
Hin1II CATG 4 cut(s) 522, 597, 797, 939
HinfI GANTC 2 cut(s) 38, 391
HpaII CCGG 1 cut(s) 104
HphI GGTGA 3 cut(s) 117, 145, 871
Hpy166II GTNNAC 2 cut(s) 113, 364
Hpy188I TCNGA 5 cut(s) 205, 354, 372, 390, 699
Hpy188III TCNNGA 4 cut(s) 63, 724, 794, 1032
Hpy8I GTNNAC 2 cut(s) 113, 364
Hpy99I CGWCG 1 cut(s) 402
HpyCH4III ACNGT 5 cut(s) 245, 429, 461, 568, 608
HpyCH4IV ACGT 1 cut(s) 1045
HpyCH4V TGCA 5 cut(s) 254, 479, 518, 737, 1028
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 197, 335, 461, 846
HpyF3I CTNAG 1 cut(s) 698
HpySE526I ACGT 1 cut(s) 1045
Hsp92II CATG 4 cut(s) 522, 597, 797, 939
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 4 cut(s) 376, 439, 712, 898
LmnI GCTCC 1 cut(s) 205
Lsp1109I GCAGC 2 cut(s) 175, 749
LweI GCATC 2 cut(s) 717, 1037
MaeI CTAG 1 cut(s) 687
MaeII ACGT 1 cut(s) 1045
MaeIII GTNAC 2 cut(s) 123, 602
MalI GATC 4 cut(s) 378, 441, 714, 900
MbiI CCGCTC 1 cut(s) 702
MboI GATC 4 cut(s) 376, 439, 712, 898
MboII GAAGA 3 cut(s) 78, 160, 788
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 1 cut(s) 466
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 5 cut(s) 117, 495, 682, 748, 758
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 860
Mox20I TGGCCA 1 cut(s) 50
Mph1103I ATGCAT 1 cut(s) 1030
MscI TGGCCA 1 cut(s) 50
MseI TTAA 5 cut(s) 408, 531, 843, 978, 1051
MslI CAYNNNNRTG 2 cut(s) 130, 965
Msp20I TGGCCA 1 cut(s) 50
MspCI CTTAAG 1 cut(s) 977
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 2 cut(s) 82, 129
MunI CAATTG 1 cut(s) 495
MvaI CCWGG 2 cut(s) 82, 129
MwoI GCNNNNNNNGC 5 cut(s) 11, 197, 335, 461, 846
NdeII GATC 4 cut(s) 376, 439, 712, 898
NlaIII CATG 4 cut(s) 522, 597, 797, 939
NlaIV GGNNCC 1 cut(s) 850
NmeAIII GCCGAG 1 cut(s) 1008
NmuCI GTSAC 2 cut(s) 123, 602
NsiI ATGCAT 1 cut(s) 1030
NspI RCATGY 1 cut(s) 939
PagI TCATGA 1 cut(s) 793
PceI AGGCCT 1 cut(s) 1041
PciI ACATGT 1 cut(s) 935
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 2 cut(s) 190, 739
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PpuMI RGGWCCY 1 cut(s) 781
PscI ACATGT 1 cut(s) 935
Psp5II RGGWCCY 1 cut(s) 781
Psp6I CCWGG 2 cut(s) 80, 127
PspFI CCCAGC 1 cut(s) 653
PspGI CCWGG 2 cut(s) 80, 127
PspN4I GGNNCC 1 cut(s) 850
PspPI GGNCC 1 cut(s) 781
PspPPI RGGWCCY 1 cut(s) 781
PstI CTGCAG 1 cut(s) 739
PsuI RGATCY 1 cut(s) 712
RsaI GTAC 1 cut(s) 313
RsaNI GTAC 1 cut(s) 312
RseI CAYNNNNRTG 2 cut(s) 130, 965
SaqAI TTAA 5 cut(s) 408, 531, 843, 978, 1051
SatI GCNGC 2 cut(s) 189, 738
Sau3AI GATC 4 cut(s) 376, 439, 712, 898
Sau96I GGNCC 1 cut(s) 781
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 2 cut(s) 82, 129
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 2 cut(s) 717, 1037
SfcI CTRYAG 3 cut(s) 735, 815, 835
SinI GGWCC 1 cut(s) 781
SmiMI CAYNNNNRTG 2 cut(s) 130, 965
SmlI CTYRAG 1 cut(s) 977
SmoI CTYRAG 1 cut(s) 977
Sse9I AATT 5 cut(s) 117, 495, 682, 748, 758
SseBI AGGCCT 1 cut(s) 1041
SsiI CCGC 3 cut(s) 329, 433, 702
SspI AATATT 2 cut(s) 77, 147
SspMI CTAG 1 cut(s) 687
StuI AGGCCT 1 cut(s) 1041
StyD4I CCNGG 2 cut(s) 80, 127
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 5 cut(s) 245, 429, 461, 568, 608
TaiI ACGT 1 cut(s) 1048
TaqI TCGA 1 cut(s) 400
TasI AATT 5 cut(s) 117, 495, 682, 748, 758
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 5 cut(s) 408, 531, 843, 978, 1051
Tru9I TTAA 5 cut(s) 408, 531, 843, 978, 1051
TscAI CASTG 3 cut(s) 304, 558, 747
TseFI GTSAC 2 cut(s) 123, 602
TseI GCWGC 2 cut(s) 188, 737
Tsp45I GTSAC 2 cut(s) 123, 602
TspDTI ATGAA 3 cut(s) 160, 359, 949
TspGWI ACGGA 1 cut(s) 784
TspRI CASTG 3 cut(s) 304, 558, 747
Vha464I CTTAAG 1 cut(s) 977
VpaK11BI GGWCC 1 cut(s) 781
XagI CCTNNNNNAGG 1 cut(s) 313
XapI RAATTY 1 cut(s) 748
XceI RCATGY 1 cut(s) 939
XmiI GTMKAC 1 cut(s) 363
XspI CTAG 1 cut(s) 687
Zsp2I ATGCAT 1 cut(s) 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.