MD09G1280100.v1.1

ALA-interacting subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001483
Physical Location & Seq
Reverse (-)
35677808 .. 35681407
3600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1280100.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGATGCAAAAGATGGAGCCTCGGATTTAACTTCCTCTACCAAAAAGAAGTCCAGAAGACCCAAATATTCCCAGTTCACACAGCAAGAGCTTCCAGCTTGCAAACCAATTTTAACACCAGCATGGGTCATACTAATATTTGTTGCAATCGGCATTGTCTTTTTACCAATTGGATTTGCTTCCTTATTTGCATCAGAGCATGTGGTGGAAATCGTCGACCACTATGATAAAGATTGCGTTCCTCCTAATTATGTGGATAATAAGCTTGCATATATTCAGAGTAGTAAAACTAACAAGACATGTATTAGGAGAGTGACGATTCCGAAACAAATGAAATCTCCCGTTTACATTTACTATCAGCTTGATCACTTCTATCAGAATCATCGCCGATATGTTAAAAGTAGAAGTGACGGTCAGTTGCGGAGCAAGTCCAATGAAAAGAAAACAGATACATGTGCTCCTGAACGTTATACAGCAAATGGTGCAATTGTTCCGTGTGGTCTAGTTGCATGGAGTTTGTTTAACGACACATACAAGTTTTCAGTGAATAACAAACAACTAGGGGTCAGCAAAAAGGACATCACATGGAAAAGTGACCCTAAGAAAAAATTTGGCTCGGACGTCTATCCTAAGAATTTTCAGAGCGGGGGTTTGATTGGAGGAGCAAAACTGAATTCTAGCATACCTGTAAATCTTCGCACCTACTTTCTGTCTTATGTTCCCCTTCTGAGTGAACAAGAAGATCTTATGGTTTGGATGCGAACTTCAGCACTGCCAACTTTCAGAAAACTATATGGGAAAATAGAAGCAGACCTTGAAGCCGATGACGTAGTAACTGTGACAATAGAGAATAATTATAACACCTACATCTTTGGTGGCAATAAGAAGCTGGTACTTTCAACCGCAAGTTGGATTGGCGGAAAGAATTGTCTACTGGGCATAGCATACCTTACTGTTGGTGGACTGTGTTTGTTCTTGGCTATAAGCTTCTTACTTCTTTATCTCTTCAAGCCAAGGCCTCTTGGGGATACATCGTACTTGTCTTGGAACAAAGGTGGACCAGGAGGACACATATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC50

Protein Analysis

360

Amino Acids

40.35

Weight (kDa)

9.41

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC50 PF03381 62 - 339 1.5e-86 LEM3 (ligand-effect modulator 3) family / CDC50 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 858
AatII GACGTC 1 cut(s) 624
AccBSI CCGCTC 1 cut(s) 645
AccI GTMKAC 2 cut(s) 216, 931
AciI CCGC 4 cut(s) 421, 645, 903, 918
AclI AACGTT 1 cut(s) 466
AcsI RAATTY 3 cut(s) 608, 634, 673
AcuI CTGAAG 1 cut(s) 750
AcyI GRCGYC 1 cut(s) 621
AfaI GTAC 2 cut(s) 894, 1037
AfiI CCNNNNNNNGG 1 cut(s) 909
AflIII ACRYGT 2 cut(s) 299, 452
AgsI TTSAA 3 cut(s) 818, 900, 1009
AjnI CCWGG 1 cut(s) 1060
AluBI AGCT 6 cut(s) 91, 98, 265, 361, 889, 987
AluI AGCT 6 cut(s) 91, 98, 265, 361, 889, 987
Alw21I GWGCWC 1 cut(s) 460
AoxI GGCC 1 cut(s) 1016
ApoI RAATTY 3 cut(s) 608, 634, 673
AspS9I GGNCC 1 cut(s) 1058
AvaII GGWCC 1 cut(s) 1058
BbsI GAAGAC 1 cut(s) 64
Bbv12I GWGCWC 1 cut(s) 460
BccI CCATC 1 cut(s) 8
BciT130I CCWGG 1 cut(s) 1062
BciVI GTATCC 1 cut(s) 1021
BclI TGATCA 1 cut(s) 364
BfaI CTAG 4 cut(s) 503, 560, 678, 1078
BfuI GTATCC 1 cut(s) 1021
BglII AGATCT 1 cut(s) 742
Bme1390I CCNGG 1 cut(s) 1062
Bme18I GGWCC 1 cut(s) 1058
BmgT120I GGNCC 1 cut(s) 1058
BmiI GGNNCC 1 cut(s) 19
BmrFI CCNGG 1 cut(s) 1062
BmrI ACTGGG 2 cut(s) 67, 944
BmsI GCATC 2 cut(s) 200, 747
BmuI ACTGGG 2 cut(s) 67, 944
BpiI GAAGAC 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 621
BsaJI CCNNGG 2 cut(s) 21, 1013
BsaXI ACNNNNNCTCC 2 cut(s) 442, 472
Bsc4I CCNNNNNNNGG 1 cut(s) 909
Bse1I ACTGG 2 cut(s) 73, 939
BseBI CCWGG 1 cut(s) 1062
BseDI CCNNGG 2 cut(s) 21, 1013
BseGI GGATG 2 cut(s) 10, 762
BseLI CCNNNNNNNGG 1 cut(s) 909
BseMII CTCAG 1 cut(s) 719
BseNI ACTGG 2 cut(s) 73, 939
BseRI GAGGAG 1 cut(s) 675
BshFI GGCC 1 cut(s) 1018
BsiHKAI GWGCWC 1 cut(s) 460
BslI CCNNNNNNNGG 1 cut(s) 909
BsnI GGCC 1 cut(s) 1018
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 2 cut(s) 364, 742
BspACI CCGC 4 cut(s) 421, 645, 903, 918
BspANI GGCC 1 cut(s) 1018
BspCNI CTCAG 1 cut(s) 720
BspLI GGNNCC 1 cut(s) 19
BsrBI CCGCTC 1 cut(s) 645
BsrI ACTGG 2 cut(s) 73, 939
BssECI CCNNGG 2 cut(s) 21, 1013
BssMI GATC 2 cut(s) 364, 742
BssNI GRCGYC 1 cut(s) 621
BssT1I CCWWGG 1 cut(s) 1013
Bst2UI CCWGG 1 cut(s) 1062
Bst4CI ACNGT 4 cut(s) 413, 838, 955, 966
Bst6I CTCTTC 1 cut(s) 1010
BstACI GRCGYC 1 cut(s) 621
BstAPI GCANNNNNTGC 1 cut(s) 482
BstC8I GCNNGC 2 cut(s) 100, 267
BstDEI CTNAG 3 cut(s) 600, 630, 728
BstF5I GGATG 2 cut(s) 10, 762
BstKTI GATC 2 cut(s) 367, 745
BstMBI GATC 2 cut(s) 364, 742
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstNI CCWGG 1 cut(s) 1062
BstNSI RCATGY 3 cut(s) 203, 303, 456
BstSCI CCNGG 1 cut(s) 1060
BstV2I GAAGAC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
BsuI GTATCC 1 cut(s) 1021
BsuRI GGCC 1 cut(s) 1018
BtgZI GCGATG 1 cut(s) 368
BtsCI GGATG 2 cut(s) 10, 762
BtsI GCAGTG 1 cut(s) 770
BtsIMutI CAGTG 2 cut(s) 549, 770
Cac8I GCNNGC 2 cut(s) 100, 267
Cfr13I GGNCC 1 cut(s) 1058
Csp6I GTAC 2 cut(s) 893, 1036
CviAII CATG 6 cut(s) 123, 200, 300, 453, 510, 585
CviQI GTAC 2 cut(s) 893, 1036
DdeI CTNAG 3 cut(s) 600, 630, 728
DpnI GATC 2 cut(s) 366, 744
DpnII GATC 2 cut(s) 364, 742
Eam1104I CTCTTC 1 cut(s) 1010
EarI CTCTTC 1 cut(s) 1010
EciI GGCGGA 1 cut(s) 933
Eco130I CCWWGG 1 cut(s) 1013
Eco147I AGGCCT 1 cut(s) 1018
Eco47I GGWCC 1 cut(s) 1058
Eco57I CTGAAG 1 cut(s) 750
EcoRI GAATTC 1 cut(s) 673
EcoRII CCWGG 1 cut(s) 1060
EcoT14I CCWWGG 1 cut(s) 1013
ErhI CCWWGG 1 cut(s) 1013
FaeI CATG 6 cut(s) 126, 203, 303, 456, 513, 588
FalI AAGNNNNNCTT 4 cut(s) 729, 761, 798, 830
FatI CATG 6 cut(s) 122, 199, 299, 452, 509, 584
FauI CCCGC 1 cut(s) 638
FbaI TGATCA 1 cut(s) 364
FblI GTMKAC 2 cut(s) 216, 931
FokI GGATG 2 cut(s) 17, 769
FspBI CTAG 4 cut(s) 503, 560, 678, 1078
HaeIII GGCC 1 cut(s) 1018
Hin1I GRCGYC 1 cut(s) 621
Hin1II CATG 6 cut(s) 126, 203, 303, 456, 513, 588
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 2 cut(s) 263, 985
HinfI GANTC 2 cut(s) 319, 379
Hpy166II GTNNAC 7 cut(s) 78, 217, 346, 734, 932, 962, 1058
Hpy188I TCNGA 9 cut(s) 25, 196, 279, 324, 378, 619, 642, 729, 785
Hpy188III TCNNGA 2 cut(s) 54, 461
Hpy8I GTNNAC 7 cut(s) 78, 217, 346, 734, 932, 962, 1058
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 1 cut(s) 734
HpyCH4III ACNGT 4 cut(s) 413, 838, 955, 966
HpyCH4IV ACGT 3 cut(s) 466, 621, 828
HpyCH4V TGCA 7 cut(s) 8, 102, 146, 191, 269, 485, 509
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 3 cut(s) 600, 630, 728
HpySE526I ACGT 3 cut(s) 466, 621, 828
Hsp92I GRCGYC 1 cut(s) 621
Hsp92II CATG 6 cut(s) 126, 203, 303, 456, 513, 588
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 2 cut(s) 364, 742
LmnI GCTCC 4 cut(s) 17, 423, 463, 662
LweI GCATC 2 cut(s) 200, 747
MaeI CTAG 4 cut(s) 503, 560, 678, 1078
MaeII ACGT 3 cut(s) 466, 621, 828
MaeIII GTNAC 5 cut(s) 313, 407, 593, 832, 838
MalI GATC 2 cut(s) 366, 744
MbiI CCGCTC 1 cut(s) 645
MboI GATC 2 cut(s) 364, 742
MboII GAAGA 4 cut(s) 69, 686, 752, 997
MfeI CAATTG 2 cut(s) 168, 486
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 1 cut(s) 460
MluCI AATT 9 cut(s) 108, 168, 247, 486, 608, 634, 673, 853, 925
MmeI TCCRAC 1 cut(s) 890
MnlI CCTC 6 cut(s) 31, 46, 252, 653, 1029, 1058
MseI TTAA 4 cut(s) 29, 113, 396, 522
MslI CAYNNNNRTG 1 cut(s) 121
MspR9I CCNGG 1 cut(s) 1062
MunI CAATTG 2 cut(s) 168, 486
MvaI CCWGG 1 cut(s) 1062
MwoI GCNNNNNNNGC 1 cut(s) 482
NdeII GATC 2 cut(s) 364, 742
NlaIII CATG 6 cut(s) 126, 203, 303, 456, 513, 588
NlaIV GGNNCC 1 cut(s) 19
NmuCI GTSAC 4 cut(s) 313, 407, 593, 838
NspI RCATGY 3 cut(s) 203, 303, 456
PceI AGGCCT 1 cut(s) 1018
PciI ACATGT 2 cut(s) 299, 452
PfeI GAWTC 2 cut(s) 319, 379
PscI ACATGT 2 cut(s) 299, 452
PsiI TTATAA 1 cut(s) 858
Psp1406I AACGTT 1 cut(s) 466
Psp6I CCWGG 1 cut(s) 1060
PspGI CCWGG 1 cut(s) 1060
PspN4I GGNNCC 1 cut(s) 19
PspPI GGNCC 1 cut(s) 1058
PsuI RGATCY 1 cut(s) 742
RsaI GTAC 2 cut(s) 894, 1037
RsaNI GTAC 2 cut(s) 893, 1036
RseI CAYNNNNRTG 1 cut(s) 121
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 4 cut(s) 29, 113, 396, 522
Sau3AI GATC 2 cut(s) 364, 742
Sau96I GGNCC 1 cut(s) 1058
ScrFI CCNGG 1 cut(s) 1062
SduI GDGCHC 1 cut(s) 460
SfaNI GCATC 2 cut(s) 200, 747
SinI GGWCC 1 cut(s) 1058
SmiMI CAYNNNNRTG 1 cut(s) 121
Sse9I AATT 9 cut(s) 108, 168, 247, 486, 608, 634, 673, 853, 925
SseBI AGGCCT 1 cut(s) 1018
SsiI CCGC 4 cut(s) 421, 645, 903, 918
SspI AATATT 2 cut(s) 68, 138
SspMI CTAG 4 cut(s) 503, 560, 678, 1078
StuI AGGCCT 1 cut(s) 1018
StyD4I CCNGG 1 cut(s) 1060
StyI CCWWGG 1 cut(s) 1013
TaaI ACNGT 4 cut(s) 413, 838, 955, 966
TaiI ACGT 3 cut(s) 469, 624, 831
TaqI TCGA 1 cut(s) 216
TasI AATT 9 cut(s) 108, 168, 247, 486, 608, 634, 673, 853, 925
TfiI GAWTC 2 cut(s) 319, 379
Tru1I TTAA 4 cut(s) 29, 113, 396, 522
Tru9I TTAA 4 cut(s) 29, 113, 396, 522
TscAI CASTG 2 cut(s) 549, 777
TseFI GTSAC 4 cut(s) 313, 407, 593, 838
Tsp45I GTSAC 4 cut(s) 313, 407, 593, 838
TspDTI ATGAA 2 cut(s) 347, 450
TspGWI ACGGA 1 cut(s) 483
TspRI CASTG 2 cut(s) 549, 777
VpaK11BI GGWCC 1 cut(s) 1058
XapI RAATTY 3 cut(s) 608, 634, 673
XceI RCATGY 3 cut(s) 203, 303, 456
XmiI GTMKAC 2 cut(s) 216, 931
XspI CTAG 4 cut(s) 503, 560, 678, 1078
ZraI GACGTC 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.