MD17G1286800.v1.1

ALA-interacting subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001483
Physical Location & Seq
Reverse (-)
34645347 .. 34648835
3489 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1286800.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGAAGCAAAAGATGGAGCCTCAAATCCAACCTCCTCTACCAAAAAGAAATCCAGAAAACCCAAATATTCCAAGTTCACACAGCAAGAGCTTCCAGCTTGCAAACCAATTTTAACACCAGCGTCGGTCATTTTAATATTTGTCGCCATCGGCATTGTCTTTATACCAATTGGACTTGCTTCCTTATTTGCATCAGAGCGTGTGGTGGAAGTCGTCCACCACTATGACAAAGATTGCATTCCTCTTAAATATGCGGATAATATGCTTGCATATATTCAGAGTAGTAAAACTAACAAGACATGCATTAGGAGACTCACTATTCCGAAACAAATGAAATCTCCTGTTTACATTTACTACCAGCTTGATCACTTCTATCAGAATCATCGCCGATATGTTAAAAGTAGAAGCGACAAGCAATTGCGGAGCAAGTCCAATGAAAATAAAACAGATGACTGTGCTCCTGAACGCTATACAACAAAGGGTGTAATTGTTCCGTGTGGTCTCGTTGCATGGAGTTTGTTTAACGACACATACAAGTTTTCAGTGAATAACAAACAACTAGGAGTCAGCAAAAAGGACATCACATGGAAAAGTGACCAAAAGAACAAATTTGGCTCAGACGTCTATCCTAAGAATTTTCAGAGCGAGGGTTTGATTGGAGGAGCAAAACTGAATTCTAGCATACCTCTGAGTGAACAAGAAGATCTTATGGTTTGGATGCGAACTGCAGCATTGCCAACTTTCAGAAAACTGTACGGGAGAATAGAAGTGGACCTTGAAGCCGATGCTGTAGTAACTGTGACAATAGAGAATAATTATAACACCTACAGCTTCCGTGGCAATAAGAAGTTGGTACTTTCAACTGCAAGCTGGATTGGCGGAAAGAATTATCTACTAGGCGTGGCATACCTTACTGTTGGTGGGCTGTGTTTGTTCTTGGCTCTAGCATTCCTACTTCTCTATCTGATCAAGCCAAGGCCTCTTGGGGATATATCGTACTTGTCTTGGAACAGGAGTGCATCAGGAGGACACATATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC50

Protein Analysis

347

Amino Acids

39.05

Weight (kDa)

9.56

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC50 PF03381 62 - 326 9e-90 LEM3 (ligand-effect modulator 3) family / CDC50 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 819
AatII GACGTC 1 cut(s) 624
AciI CCGC 3 cut(s) 254, 421, 879
AcsI RAATTY 3 cut(s) 608, 634, 673
AcyI GRCGYC 1 cut(s) 621
AfaI GTAC 3 cut(s) 755, 855, 998
AgsI TTSAA 2 cut(s) 779, 861
AluBI AGCT 5 cut(s) 91, 98, 361, 831, 870
AluI AGCT 5 cut(s) 91, 98, 361, 831, 870
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 2 cut(s) 304, 506
AoxI GGCC 1 cut(s) 977
ApeKI GCWGC 1 cut(s) 728
ApoI RAATTY 3 cut(s) 608, 634, 673
AspS9I GGNCC 1 cut(s) 772
AvaII GGWCC 1 cut(s) 772
Bbv12I GWGCWC 1 cut(s) 460
BbvI GCAGC 1 cut(s) 740
BccI CCATC 2 cut(s) 8, 155
BclI TGATCA 2 cut(s) 364, 966
BcoDI GTCTC 2 cut(s) 304, 506
BfaI CTAG 5 cut(s) 560, 678, 896, 944, 1039
BfmI CTRYAG 3 cut(s) 726, 789, 826
BglII AGATCT 1 cut(s) 703
BisI GCNGC 1 cut(s) 729
BlsI GCNGC 1 cut(s) 730
Bme18I GGWCC 1 cut(s) 772
BmgT120I GGNCC 1 cut(s) 772
BmiI GGNNCC 1 cut(s) 19
BmsI GCATC 4 cut(s) 200, 708, 775, 1028
BsaHI GRCGYC 1 cut(s) 621
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 2 cut(s) 835, 974
Bse3DI GCAATG 1 cut(s) 731
BseDI CCNNGG 2 cut(s) 835, 974
BseGI GGATG 1 cut(s) 723
BseMI GCAATG 1 cut(s) 731
BseMII CTCAG 2 cut(s) 630, 680
BseRI GAGGAG 2 cut(s) 25, 675
BseXI GCAGC 1 cut(s) 740
BshFI GGCC 1 cut(s) 979
BsiHKAI GWGCWC 1 cut(s) 460
BsmAI GTCTC 2 cut(s) 304, 506
BsmI GAATGC 2 cut(s) 237, 947
BsnI GGCC 1 cut(s) 979
Bso31I GGTCTC 1 cut(s) 506
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 3 cut(s) 364, 703, 966
BspACI CCGC 3 cut(s) 254, 421, 879
BspANI GGCC 1 cut(s) 979
BspCNI CTCAG 2 cut(s) 629, 681
BspLI GGNNCC 1 cut(s) 19
BspMAI CTGCAG 1 cut(s) 730
BspTNI GGTCTC 1 cut(s) 506
BsrDI GCAATG 1 cut(s) 731
BssECI CCNNGG 2 cut(s) 835, 974
BssMI GATC 3 cut(s) 364, 703, 966
BssNI GRCGYC 1 cut(s) 621
BssT1I CCWWGG 1 cut(s) 974
Bst4CI ACNGT 4 cut(s) 455, 753, 799, 916
BstACI GRCGYC 1 cut(s) 621
BstC8I GCNNGC 3 cut(s) 100, 267, 868
BstDEI CTNAG 3 cut(s) 616, 630, 689
BstDSI CCRYGG 1 cut(s) 835
BstF5I GGATG 1 cut(s) 723
BstKTI GATC 3 cut(s) 367, 706, 969
BstMAI GTCTC 2 cut(s) 304, 506
BstMBI GATC 3 cut(s) 364, 703, 966
BstMWI GCNNNNNNNGC 2 cut(s) 837, 876
BstNSI RCATGY 1 cut(s) 303
BstSFI CTRYAG 3 cut(s) 726, 789, 826
BstV1I GCAGC 1 cut(s) 740
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
BsuRI GGCC 1 cut(s) 979
BtgI CCRYGG 1 cut(s) 835
BtgZI GCGATG 1 cut(s) 368
BtsCI GGATG 1 cut(s) 723
BtsIMutI CAGTG 1 cut(s) 549
Cac8I GCNNGC 3 cut(s) 100, 267, 868
Cfr13I GGNCC 1 cut(s) 772
CseI GACGC 1 cut(s) 111
Csp6I GTAC 3 cut(s) 754, 854, 997
CviAII CATG 3 cut(s) 300, 510, 585
CviQI GTAC 3 cut(s) 754, 854, 997
DdeI CTNAG 3 cut(s) 616, 630, 689
DpnI GATC 3 cut(s) 366, 705, 968
DpnII GATC 3 cut(s) 364, 703, 966
EciI GGCGGA 1 cut(s) 894
Eco130I CCWWGG 1 cut(s) 974
Eco147I AGGCCT 1 cut(s) 979
Eco31I GGTCTC 1 cut(s) 506
Eco47I GGWCC 1 cut(s) 772
EcoRI GAATTC 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 974
EcoT22I ATGCAT 1 cut(s) 305
ErhI CCWWGG 1 cut(s) 974
FaeI CATG 3 cut(s) 303, 513, 588
FalI AAGNNNNNCTT 4 cut(s) 690, 722, 759, 791
FatI CATG 3 cut(s) 299, 509, 584
FbaI TGATCA 2 cut(s) 364, 966
Fnu4HI GCNGC 1 cut(s) 729
FokI GGATG 1 cut(s) 730
Fsp4HI GCNGC 1 cut(s) 729
FspBI CTAG 5 cut(s) 560, 678, 896, 944, 1039
GluI GCNGC 1 cut(s) 729
HaeIII GGCC 1 cut(s) 979
HgaI GACGC 1 cut(s) 111
Hin1I GRCGYC 1 cut(s) 621
Hin1II CATG 3 cut(s) 303, 513, 588
HinfI GANTC 3 cut(s) 312, 379, 564
Hpy166II GTNNAC 5 cut(s) 78, 217, 346, 695, 772
Hpy188I TCNGA 9 cut(s) 196, 279, 324, 378, 619, 642, 690, 746, 966
Hpy188III TCNNGA 3 cut(s) 54, 461, 1023
Hpy8I GTNNAC 5 cut(s) 78, 217, 346, 695, 772
Hpy99I CGWCG 1 cut(s) 127
HpyCH4III ACNGT 4 cut(s) 455, 753, 799, 916
HpyCH4IV ACGT 1 cut(s) 621
HpyCH4V TGCA 9 cut(s) 102, 191, 237, 269, 303, 509, 728, 866, 1019
HpyF10VI GCNNNNNNNGC 2 cut(s) 837, 876
HpyF3I CTNAG 3 cut(s) 616, 630, 689
HpySE526I ACGT 1 cut(s) 621
Hsp92I GRCGYC 1 cut(s) 621
Hsp92II CATG 3 cut(s) 303, 513, 588
Ksp22I TGATCA 2 cut(s) 364, 966
Kzo9I GATC 3 cut(s) 364, 703, 966
LmnI GCTCC 4 cut(s) 17, 423, 463, 662
LpnPI CCDG 9 cut(s) 67, 108, 132, 354, 371, 474, 856, 997, 1008
Lsp1109I GCAGC 1 cut(s) 740
LweI GCATC 4 cut(s) 200, 708, 775, 1028
MaeI CTAG 5 cut(s) 560, 678, 896, 944, 1039
MaeII ACGT 1 cut(s) 621
MaeIII GTNAC 3 cut(s) 593, 793, 799
MalI GATC 3 cut(s) 366, 705, 968
MboI GATC 3 cut(s) 364, 703, 966
MboII GAAGA 1 cut(s) 713
MfeI CAATTG 2 cut(s) 168, 416
MflI RGATCY 1 cut(s) 703
MhlI GDGCHC 1 cut(s) 460
MluCI AATT 9 cut(s) 108, 168, 416, 486, 608, 634, 673, 814, 886
MlyI GAGTC 2 cut(s) 306, 573
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 9 cut(s) 31, 43, 46, 252, 640, 653, 696, 990, 1019
Mph1103I ATGCAT 1 cut(s) 305
MseI TTAA 5 cut(s) 113, 134, 246, 396, 522
MslI CAYNNNNRTG 1 cut(s) 222
MunI CAATTG 2 cut(s) 168, 416
Mva1269I GAATGC 2 cut(s) 237, 947
MwoI GCNNNNNNNGC 2 cut(s) 837, 876
NdeII GATC 3 cut(s) 364, 703, 966
NlaIII CATG 3 cut(s) 303, 513, 588
NlaIV GGNNCC 1 cut(s) 19
NmuCI GTSAC 2 cut(s) 593, 799
NsiI ATGCAT 1 cut(s) 305
NspI RCATGY 1 cut(s) 303
PceI AGGCCT 1 cut(s) 979
PctI GAATGC 2 cut(s) 237, 947
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 1 cut(s) 730
PleI GAGTC 2 cut(s) 306, 572
PpsI GAGTC 2 cut(s) 306, 572
PsiI TTATAA 1 cut(s) 819
PspN4I GGNNCC 1 cut(s) 19
PspPI GGNCC 1 cut(s) 772
PstI CTGCAG 1 cut(s) 730
PsuI RGATCY 1 cut(s) 703
RsaI GTAC 3 cut(s) 755, 855, 998
RsaNI GTAC 3 cut(s) 754, 854, 997
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 5 cut(s) 113, 134, 246, 396, 522
SatI GCNGC 1 cut(s) 729
Sau3AI GATC 3 cut(s) 364, 703, 966
Sau96I GGNCC 1 cut(s) 772
SchI GAGTC 2 cut(s) 306, 573
SduI GDGCHC 1 cut(s) 460
SfaNI GCATC 4 cut(s) 200, 708, 775, 1028
SfcI CTRYAG 3 cut(s) 726, 789, 826
SinI GGWCC 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 222
Sse9I AATT 9 cut(s) 108, 168, 416, 486, 608, 634, 673, 814, 886
SseBI AGGCCT 1 cut(s) 979
SsiI CCGC 3 cut(s) 254, 421, 879
SspI AATATT 2 cut(s) 68, 138
SspMI CTAG 5 cut(s) 560, 678, 896, 944, 1039
StuI AGGCCT 1 cut(s) 979
StyI CCWWGG 1 cut(s) 974
TaaI ACNGT 4 cut(s) 455, 753, 799, 916
TaiI ACGT 1 cut(s) 624
TaqII GACCGA 1 cut(s) 115
TasI AATT 9 cut(s) 108, 168, 416, 486, 608, 634, 673, 814, 886
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 5 cut(s) 113, 134, 246, 396, 522
Tru9I TTAA 5 cut(s) 113, 134, 246, 396, 522
TscAI CASTG 1 cut(s) 549
TseFI GTSAC 2 cut(s) 593, 799
TseI GCWGC 1 cut(s) 728
Tsp45I GTSAC 2 cut(s) 593, 799
TspDTI ATGAA 2 cut(s) 347, 450
TspGWI ACGGA 2 cut(s) 483, 824
TspRI CASTG 1 cut(s) 549
VpaK11BI GGWCC 1 cut(s) 772
XapI RAATTY 3 cut(s) 608, 634, 673
XceI RCATGY 1 cut(s) 303
XspI CTAG 5 cut(s) 560, 678, 896, 944, 1039
ZraI GACGTC 1 cut(s) 622
Zsp2I ATGCAT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.