MD10G1216500.v1.1

ALA-interacting subunit 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001483
Physical Location & Seq
Reverse (-)
31492397 .. 31496924
4528 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1216500.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGAGTTCCAATACGGCGCCGTCTAGCTCAGGAGGCGCCGGATCTGCGGATGCAACGTCTACCAGGAGGCATTCCAAGCGACCTAAATATTCAAGATTTACTCAGCAGGAACTTCCAGCGTGCAAGCCAATTCTCACGCCACGATGGGTGATCTCAGCATTCCTGCTCGTAAGCGCTGTCTTCATTCCCATCGGAGTTGTTTCCCTCTTTGCTTCTCGAGATGTTGTTGAAATTATTGATCGGTATGAAACTGATTGCATACCAACACCCTCTAGAGATGATAAGGTCAGGTTCATACAAGGGCCTGGAGTAAAAACATGCAACAGAACTCTAAGGGTATCAAAGCATATGAAGCAGCCTATATATGTATATTACCAGCTTGACAATTTCTACCAGAATCATCGCAGATATGTTAAGAGTCGAAGTGATCAACAGTTGAAAGATTCAAAGTCTGAGAGTGAAATAGATGCCTGTAAGCCTGAAGATAAGGCAAATGGTCTACCTATTGTACCTTGTGGTCTTATTGCTTGGAGTTTGTTCAACGACACCTATACTTTTTCCCGCAATAGCCAGCAGTTGACGGTGAACAAGAAGGATATCTCGTGGAAGAGTGATAGGGAGCACAAATTTGGCAAAAATGTCTTTCCAAAGAACTTTCAGAATGGAACTCTTAAAGGTGGCAAGTCTCTGGATACCTCTAAACCATTAAGTGAGCAAGAGGACCTTATTGTTTGGATGAGAACTGCTGCACTACCGACATTTAGAAAGCTGTACGGGAAGATAGAGGTAGATCTGAAAGAAAATGATACGATAGAAGTGACACTGGAGAACAATTACAACACATACAGTTTTAATGGAAAAAAGAAGCTTGTGCTCTCGACCACCAGTTGGATTGGTGGCAGGAATGACTTCCTTGGCATTGCTTATCTCACTGTTGGAGGGTTGTGCTTCTTTCTGGCCATGGCTTTCACTGTTGTGTATCTTGTTAAGCCAAGGCAACTTGGAGACCCATCGTATTTGTCGTGGAACAGAAATCCGGGAGGTCACTAA

Protein Analysis

350

Amino Acids

39.47

Weight (kDa)

9.51

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC50 PF03381 69 - 333 2.3e-91 LEM3 (ligand-effect modulator 3) family / CDC50 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 16, 35
AccB7I CCANNNNNTGG 1 cut(s) 888
AccI GTMKAC 2 cut(s) 59, 499
AciI CCGC 2 cut(s) 47, 562
AclWI GGATC 1 cut(s) 49
AcoI YGGCCR 1 cut(s) 957
AcsI RAATTY 1 cut(s) 626
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 2 cut(s) 17, 36
AfaI GTAC 2 cut(s) 510, 773
AfeI AGCGCT 1 cut(s) 175
AfiI CCNNNNNNNGG 1 cut(s) 888
AgsI TTSAA 5 cut(s) 93, 230, 439, 447, 541
AjnI CCWGG 2 cut(s) 62, 304
AleI CACNNNNGTG 1 cut(s) 974
AluBI AGCT 4 cut(s) 27, 379, 769, 868
AluI AGCT 4 cut(s) 27, 379, 769, 868
Alw21I GWGCWC 2 cut(s) 624, 876
Alw26I GTCTC 2 cut(s) 690, 999
AlwI GGATC 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 216
Aor51HI AGCGCT 1 cut(s) 175
AoxI GGCC 2 cut(s) 302, 957
ApeKI GCWGC 2 cut(s) 355, 746
ApoI RAATTY 1 cut(s) 626
Asp700I GAANNNNTTC 1 cut(s) 908
AspLEI GCGC 3 cut(s) 19, 38, 176
AspS9I GGNCC 2 cut(s) 302, 721
AsuC2I CCSGG 1 cut(s) 1038
AsuHPI GGTGA 2 cut(s) 160, 595
AvaI CYCGRG 1 cut(s) 216
AvaII GGWCC 1 cut(s) 721
BalI TGGCCA 1 cut(s) 959
BanI GGYRCC 2 cut(s) 16, 35
BauI CACGAG 1 cut(s) 601
BbsI GAAGAC 1 cut(s) 172
Bbv12I GWGCWC 2 cut(s) 624, 876
BbvI GCAGC 2 cut(s) 367, 733
BccI CCATC 3 cut(s) 138, 197, 1018
BceAI ACGGC 2 cut(s) 4, 30
BciT130I CCWGG 2 cut(s) 64, 306
BciVI GTATCC 1 cut(s) 685
BclI TGATCA 1 cut(s) 427
BcnI CCSGG 1 cut(s) 1038
BcoDI GTCTC 2 cut(s) 690, 999
BfaI CTAG 2 cut(s) 24, 273
BfoI RGCGCY 3 cut(s) 20, 39, 177
BfuI GTATCC 1 cut(s) 685
BglII AGATCT 1 cut(s) 790
BisI GCNGC 2 cut(s) 356, 747
BlsI GCNGC 2 cut(s) 357, 748
Bme1390I CCNGG 3 cut(s) 64, 306, 1038
Bme18I GGWCC 1 cut(s) 721
BmeT110I CYCGRG 1 cut(s) 216
BmgT120I GGNCC 2 cut(s) 302, 721
BmiI GGNNCC 2 cut(s) 18, 37
BmrFI CCNGG 3 cut(s) 64, 306, 1038
BmsI GCATC 2 cut(s) 40, 457
BpiI GAAGAC 1 cut(s) 172
BpmI CTGGAG 2 cut(s) 327, 845
Bpu10I CCTNAGC 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 1038
BsaHI GRCGYC 2 cut(s) 17, 36
BsaI GGTCTC 1 cut(s) 999
BsaJI CCNNGG 3 cut(s) 913, 960, 992
Bsc4I CCNNNNNNNGG 1 cut(s) 888
Bse1I ACTGG 2 cut(s) 828, 885
Bse3DI GCAATG 1 cut(s) 918
BseBI CCWGG 2 cut(s) 64, 306
BseDI CCNNGG 3 cut(s) 913, 960, 992
BseGI GGATG 2 cut(s) 55, 741
BseLI CCNNNNNNNGG 1 cut(s) 888
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 4 cut(s) 42, 116, 168, 444
BseNI ACTGG 2 cut(s) 828, 885
BseXI GCAGC 2 cut(s) 367, 733
BsgI GTGCAG 1 cut(s) 732
BshFI GGCC 2 cut(s) 304, 959
BshNI GGYRCC 2 cut(s) 16, 35
BsiHKAI GWGCWC 2 cut(s) 624, 876
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 2 cut(s) 39, 1037
BslI CCNNNNNNNGG 1 cut(s) 888
BsmAI GTCTC 2 cut(s) 690, 999
BsmI GAATGC 2 cut(s) 70, 158
BsnI GGCC 2 cut(s) 304, 959
Bso31I GGTCTC 1 cut(s) 999
BsoBI CYCGRG 1 cut(s) 216
Bsp1286I GDGCHC 2 cut(s) 624, 876
Bsp143I GATC 5 cut(s) 41, 150, 238, 427, 790
Bsp19I CCATGG 1 cut(s) 960
BspACI CCGC 2 cut(s) 47, 562
BspANI GGCC 2 cut(s) 304, 959
BspCNI CTCAG 4 cut(s) 41, 115, 167, 445
BspLI GGNNCC 2 cut(s) 18, 37
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 2 cut(s) 16, 35
BspTNI GGTCTC 1 cut(s) 999
BsrDI GCAATG 1 cut(s) 918
BsrI ACTGG 2 cut(s) 828, 885
BssECI CCNNGG 3 cut(s) 913, 960, 992
BssMI GATC 5 cut(s) 41, 150, 238, 427, 790
BssNI GRCGYC 2 cut(s) 17, 36
BssSI CACGAG 1 cut(s) 601
BssT1I CCWWGG 3 cut(s) 913, 960, 992
Bst2BI CACGAG 1 cut(s) 601
Bst2UI CCWGG 2 cut(s) 64, 306
Bst4CI ACNGT 5 cut(s) 435, 583, 848, 934, 973
Bst6I CTCTTC 1 cut(s) 602
BstACI GRCGYC 2 cut(s) 17, 36
BstC8I GCNNGC 3 cut(s) 121, 125, 572
BstDEI CTNAG 5 cut(s) 28, 102, 154, 332, 453
BstDSI CCRYGG 1 cut(s) 960
BstF5I GGATG 2 cut(s) 55, 741
BstH2I RGCGCY 3 cut(s) 20, 39, 177
BstHHI GCGC 3 cut(s) 19, 38, 176
BstKTI GATC 5 cut(s) 44, 153, 241, 430, 793
BstMAI GTCTC 2 cut(s) 690, 999
BstMBI GATC 5 cut(s) 41, 150, 238, 427, 790
BstMWI GCNNNNNNNGC 4 cut(s) 33, 44, 76, 352
BstNI CCWGG 2 cut(s) 64, 306
BstNSI RCATGY 1 cut(s) 321
BstSCI CCNGG 3 cut(s) 62, 304, 1036
BstV1I GCAGC 2 cut(s) 367, 733
BstV2I GAAGAC 1 cut(s) 172
BstX2I RGATCY 2 cut(s) 41, 790
BstYI RGATCY 2 cut(s) 41, 790
BsuI GTATCC 1 cut(s) 685
BsuRI GGCC 2 cut(s) 304, 959
BtgI CCRYGG 1 cut(s) 960
BtgZI GCGATG 1 cut(s) 386
BtsCI GGATG 2 cut(s) 55, 741
BtsIMutI CAGTG 3 cut(s) 821, 930, 969
Cac8I GCNNGC 3 cut(s) 121, 125, 572
CfoI GCGC 3 cut(s) 19, 38, 176
Cfr13I GGNCC 2 cut(s) 302, 721
Csp6I GTAC 2 cut(s) 509, 772
CviAII CATG 2 cut(s) 318, 961
CviQI GTAC 2 cut(s) 509, 772
DdeI CTNAG 5 cut(s) 28, 102, 154, 332, 453
DinI GGCGCC 2 cut(s) 18, 37
DpnI GATC 5 cut(s) 43, 152, 240, 429, 792
DpnII GATC 5 cut(s) 41, 150, 238, 427, 790
EaeI YGGCCR 1 cut(s) 957
Eam1104I CTCTTC 1 cut(s) 602
EarI CTCTTC 1 cut(s) 602
Eco130I CCWWGG 3 cut(s) 913, 960, 992
Eco31I GGTCTC 1 cut(s) 999
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 1 cut(s) 721
Eco47III AGCGCT 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 216
EcoO109I RGGNCCY 2 cut(s) 302, 721
EcoRII CCWGG 2 cut(s) 62, 304
EcoRV GATATC 1 cut(s) 598
EcoT14I CCWWGG 3 cut(s) 913, 960, 992
EgeI GGCGCC 2 cut(s) 18, 37
EheI GGCGCC 2 cut(s) 18, 37
ErhI CCWWGG 3 cut(s) 913, 960, 992
FaeI CATG 2 cut(s) 321, 964
FalI AAGNNNNNCTT 2 cut(s) 708, 740
FatI CATG 2 cut(s) 317, 960
FauI CCCGC 1 cut(s) 569
FauNDI CATATG 1 cut(s) 348
FbaI TGATCA 1 cut(s) 427
FblI GTMKAC 2 cut(s) 59, 499
Fnu4HI GCNGC 2 cut(s) 356, 747
FokI GGATG 2 cut(s) 62, 748
Fsp4HI GCNGC 2 cut(s) 356, 747
FspBI CTAG 2 cut(s) 24, 273
GlaI GCGC 3 cut(s) 18, 37, 175
GluI GCNGC 2 cut(s) 356, 747
GsuI CTGGAG 2 cut(s) 327, 845
HaeII RGCGCY 3 cut(s) 20, 39, 177
HaeIII GGCC 2 cut(s) 304, 959
HapII CCGG 2 cut(s) 39, 1037
HhaI GCGC 3 cut(s) 19, 38, 176
Hin1I GRCGYC 2 cut(s) 17, 36
Hin1II CATG 2 cut(s) 321, 964
Hin6I GCGC 3 cut(s) 17, 36, 174
HinP1I GCGC 3 cut(s) 17, 36, 174
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HindIII AAGCTT 1 cut(s) 866
HinfI GANTC 3 cut(s) 397, 418, 443
HpaII CCGG 2 cut(s) 39, 1037
HphI GGTGA 2 cut(s) 160, 595
Hpy166II GTNNAC 4 cut(s) 60, 500, 579, 586
Hpy188I TCNGA 4 cut(s) 194, 454, 660, 795
Hpy188III TCNNGA 7 cut(s) 30, 93, 216, 218, 273, 689, 877
Hpy8I GTNNAC 4 cut(s) 60, 500, 579, 586
HpyAV CCTTC 1 cut(s) 586
HpyCH4III ACNGT 5 cut(s) 435, 583, 848, 934, 973
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 5 cut(s) 53, 123, 258, 321, 749
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 44, 76, 352
HpyF3I CTNAG 5 cut(s) 28, 102, 154, 332, 453
HpySE526I ACGT 1 cut(s) 56
Hsp92I GRCGYC 2 cut(s) 17, 36
Hsp92II CATG 2 cut(s) 321, 964
HspAI GCGC 3 cut(s) 17, 36, 174
KasI GGCGCC 2 cut(s) 16, 35
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 5 cut(s) 41, 150, 238, 427, 790
LmnI GCTCC 1 cut(s) 619
Lsp1109I GCAGC 2 cut(s) 367, 733
LweI GCATC 2 cut(s) 40, 457
MaeI CTAG 2 cut(s) 24, 273
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 2 cut(s) 817, 1043
MalI GATC 5 cut(s) 43, 152, 240, 429, 792
MboI GATC 5 cut(s) 41, 150, 238, 427, 790
MboII GAAGA 4 cut(s) 172, 494, 619, 790
MflI RGATCY 2 cut(s) 41, 790
MhlI GDGCHC 2 cut(s) 624, 876
MlsI TGGCCA 1 cut(s) 959
MluCI AATT 5 cut(s) 129, 231, 385, 626, 832
MluNI TGGCCA 1 cut(s) 959
Mly113I GGCGCC 2 cut(s) 17, 36
MlyI GAGTC 1 cut(s) 427
MmeI TCCRAC 2 cut(s) 869, 916
MnlI CCTC 9 cut(s) 26, 60, 215, 280, 706, 712, 778, 932, 1034
Mox20I TGGCCA 1 cut(s) 959
MroXI GAANNNNTTC 1 cut(s) 908
MscI TGGCCA 1 cut(s) 959
MseI TTAA 5 cut(s) 414, 672, 707, 852, 987
MslI CAYNNNNRTG 1 cut(s) 974
Msp20I TGGCCA 1 cut(s) 959
MspI CCGG 2 cut(s) 39, 1037
MspR9I CCNGG 3 cut(s) 64, 306, 1038
Mva1269I GAATGC 2 cut(s) 70, 158
MvaI CCWGG 2 cut(s) 64, 306
MwoI GCNNNNNNNGC 4 cut(s) 33, 44, 76, 352
NarI GGCGCC 2 cut(s) 17, 36
NciI CCSGG 1 cut(s) 1038
NcoI CCATGG 1 cut(s) 960
NdeI CATATG 1 cut(s) 348
NdeII GATC 5 cut(s) 41, 150, 238, 427, 790
NlaIII CATG 2 cut(s) 321, 964
NlaIV GGNNCC 2 cut(s) 18, 37
NmuCI GTSAC 2 cut(s) 817, 1043
NspI RCATGY 1 cut(s) 321
OliI CACNNNNGTG 1 cut(s) 974
PaeR7I CTCGAG 1 cut(s) 216
PcsI WCGNNNNNNNCGW 1 cut(s) 1019
PctI GAATGC 2 cut(s) 70, 158
PdmI GAANNNNTTC 1 cut(s) 908
PfeI GAWTC 2 cut(s) 397, 443
PflMI CCANNNNNTGG 1 cut(s) 888
PfoI TCCNGGA 1 cut(s) 1036
PkrI GCNGC 2 cut(s) 357, 748
PleI GAGTC 1 cut(s) 426
PluTI GGCGCC 2 cut(s) 20, 39
PpsI GAGTC 1 cut(s) 426
PpuMI RGGWCCY 1 cut(s) 721
Psp5II RGGWCCY 1 cut(s) 721
Psp6I CCWGG 2 cut(s) 62, 304
PspGI CCWGG 2 cut(s) 62, 304
PspN4I GGNNCC 2 cut(s) 18, 37
PspPI GGNCC 2 cut(s) 302, 721
PspPPI RGGWCCY 1 cut(s) 721
PsuI RGATCY 2 cut(s) 41, 790
RsaI GTAC 2 cut(s) 510, 773
RsaNI GTAC 2 cut(s) 509, 772
RseI CAYNNNNRTG 1 cut(s) 974
SaqAI TTAA 5 cut(s) 414, 672, 707, 852, 987
SatI GCNGC 2 cut(s) 356, 747
Sau3AI GATC 5 cut(s) 41, 150, 238, 427, 790
Sau96I GGNCC 2 cut(s) 302, 721
SchI GAGTC 1 cut(s) 427
ScrFI CCNGG 3 cut(s) 64, 306, 1038
SduI GDGCHC 2 cut(s) 624, 876
SfaNI GCATC 2 cut(s) 40, 457
SfoI GGCGCC 2 cut(s) 18, 37
Sfr274I CTCGAG 1 cut(s) 216
SinI GGWCC 1 cut(s) 721
SlaI CTCGAG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 974
SmlI CTYRAG 1 cut(s) 216
SmoI CTYRAG 1 cut(s) 216
Sse9I AATT 5 cut(s) 129, 231, 385, 626, 832
SsiI CCGC 2 cut(s) 47, 562
SspDI GGCGCC 2 cut(s) 16, 35
SspI AATATT 1 cut(s) 89
SspMI CTAG 2 cut(s) 24, 273
StyD4I CCNGG 3 cut(s) 62, 304, 1036
StyI CCWWGG 3 cut(s) 913, 960, 992
TaaI ACNGT 5 cut(s) 435, 583, 848, 934, 973
TaiI ACGT 1 cut(s) 59
TaqI TCGA 3 cut(s) 217, 421, 878
TasI AATT 5 cut(s) 129, 231, 385, 626, 832
TfiI GAWTC 2 cut(s) 397, 443
Tru1I TTAA 5 cut(s) 414, 672, 707, 852, 987
Tru9I TTAA 5 cut(s) 414, 672, 707, 852, 987
TscAI CASTG 3 cut(s) 828, 937, 976
TseFI GTSAC 2 cut(s) 817, 1043
TseI GCWGC 2 cut(s) 355, 746
Tsp45I GTSAC 2 cut(s) 817, 1043
TspDTI ATGAA 4 cut(s) 172, 261, 283, 365
TspRI CASTG 3 cut(s) 828, 937, 976
Van91I CCANNNNNTGG 1 cut(s) 888
VpaK11BI GGWCC 1 cut(s) 721
XapI RAATTY 1 cut(s) 626
XbaI TCTAGA 1 cut(s) 272
XceI RCATGY 1 cut(s) 321
XhoI CTCGAG 1 cut(s) 216
XmiI GTMKAC 2 cut(s) 59, 499
XmnI GAANNNNTTC 1 cut(s) 908
XspI CTAG 2 cut(s) 24, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.