Rw3G025600

ALA-interacting subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001483
Physical Location & Seq
Forward (+)
34373220 .. 34376347
3128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025600.1

Sequence Viewer

Length: 1056 bp
ATGACCCCAAAAGGTGACAAGGGCAAAGAAGGAGACTCGTCCTTTGCCAAAAAGAAGTCCAGAAGACCCAAATATTCCAGGTTCTCACAGCAAGAGCTTCCAGCTTGCAAACCAATTTTGTCACCGGGATGGGTGGTTTCAATATTCATCACCATTGGCATTGTCTTTATCCCAATTGGCTTTGCTGCCTTATTCGCCTCAGAGCGTGTTGTGGAACTACAATTCCAATATGACCAAGATTGTGTTCCTGCAAAGTATAAGGATAATACGGTTGCATATATTCAAAGCAATGACACTGACAAGACCTGCTCGAGGAAAATGCCTGCTGTCACAAGTAAAATGAAATCTCCCATTTATGTCTACTATCAGATTGATCACTACTTTCAGAATCATCGCCGATATGTTAAAAGTAGAAGTGACGATCAGTTGCGGAGCGTGGCAAAGGAAAATAGCACAAGCGACTGTGCGCCTGAACGCTTTGCGAAAAATGGTCAACCAATTGTTCCTTGTGGCCTCGTTGCATGGAGTTTGTTTAATGACACATATAAGTTTTCAGTGAAAAACAAAACAATAGCGGTCAGCAAAAAGGACATCACATGGAAAAGTGACCGTGAGAAAAAGTTTTCAAGCAGTGTCTATCCTAAGAACTTTCAGTCTGGGGGTTTGATTGGAGGAGCAAAGCTCAATTCTAGCATACCTCTGAGCCAACAAGAGGATCTTATTGTTTGGATGCGAACTGCAGCAATGCCATCTTTTAGAAAACTGTATGGGAGGATAGAAGTGGACCTTGAGGCTCATGATGAAATAACAATAGAACTACAGAATAACTATAATACCTACAGCTTTAAAGGCAGCAAGTCAGTGATACTTTCTACTGCGAGTTGGATTGGTGGAAAGAATGATCTAATGGGCATTGCATACCTTACCATTGGCGGAATGTGTTTGTTCTTGGCAATATGCTTCATACTTATGTATGTGCTCAAGCCGAGACCTCTTGGGGATACCTCCTACTTGTCTTGGAATAGAAATGCATCAGGAGGACGCCTAAATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC50

Protein Analysis

351

Amino Acids

39.62

Weight (kDa)

9.49

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC50 PF03381 64 - 330 1.2e-85 LEM3 (ligand-effect modulator 3) family / CDC50 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 314
AccI GTMKAC 1 cut(s) 360
AciI CCGC 3 cut(s) 430, 575, 933
AclWI GGATC 1 cut(s) 723
AcsI RAATTY 1 cut(s) 1048
AcyI GRCGYC 1 cut(s) 1042
AfiI CCNNNNNNNGG 2 cut(s) 312, 712
AgsI TTSAA 3 cut(s) 141, 284, 627
AjnI CCWGG 1 cut(s) 77
AjuI GAANNNNNNNTTGG 2 cut(s) 228, 260
AloI GAACNNNNNNTCC 2 cut(s) 207, 239
AluBI AGCT 4 cut(s) 97, 104, 682, 843
AluI AGCT 4 cut(s) 97, 104, 682, 843
Alw21I GWGCWC 1 cut(s) 981
Alw26I GTCTC 2 cut(s) 27, 982
AlwI GGATC 1 cut(s) 723
Ama87I CYCGRG 1 cut(s) 310
AoxI GGCC 1 cut(s) 511
ApeKI GCWGC 3 cut(s) 185, 740, 852
ApoI RAATTY 1 cut(s) 1048
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspLEI GCGC 1 cut(s) 469
AspS9I GGNCC 1 cut(s) 784
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 3 cut(s) 26, 114, 142
AvaI CYCGRG 1 cut(s) 310
AvaII GGWCC 1 cut(s) 784
BbsI GAAGAC 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 981
BbvI GCAGC 3 cut(s) 172, 752, 864
BccI CCATC 2 cut(s) 123, 757
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciT130I CCWGG 1 cut(s) 79
BciVI GTATCC 1 cut(s) 994
BclI TGATCA 1 cut(s) 373
BcnI CCSGG 1 cut(s) 126
BcoDI GTCTC 2 cut(s) 27, 982
BfaI CTAG 1 cut(s) 690
BfmI CTRYAG 3 cut(s) 738, 818, 838
BfuAI ACCTGC 1 cut(s) 314
BfuI GTATCC 1 cut(s) 994
BisI GCNGC 3 cut(s) 186, 741, 853
BlsI GCNGC 3 cut(s) 187, 742, 854
Bme1390I CCNGG 2 cut(s) 79, 126
Bme18I GGWCC 1 cut(s) 784
BmeT110I CYCGRG 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 784
BmrFI CCNGG 2 cut(s) 79, 126
BmsI GCATC 2 cut(s) 720, 1040
BpiI GAAGAC 1 cut(s) 70
BplI GAGNNNNNCTC 2 cut(s) 666, 698
BpuEI CTTGAG 2 cut(s) 809, 965
BpuMI CCSGG 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 1042
BsaI GGTCTC 1 cut(s) 982
Bsc4I CCNNNNNNNGG 2 cut(s) 312, 712
Bse3DI GCAATG 3 cut(s) 295, 750, 912
BseBI CCWGG 1 cut(s) 79
BseGI GGATG 2 cut(s) 134, 735
BseLI CCNNNNNNNGG 2 cut(s) 312, 712
BseMI GCAATG 3 cut(s) 295, 750, 912
BseMII CTCAG 2 cut(s) 213, 692
BseRI GAGGAG 1 cut(s) 687
BseXI GCAGC 3 cut(s) 172, 752, 864
BshFI GGCC 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 981
BsiHKCI CYCGRG 1 cut(s) 310
BsiSI CCGG 1 cut(s) 125
BslI CCNNNNNNNGG 2 cut(s) 312, 712
BsmAI GTCTC 2 cut(s) 27, 982
BsnI GGCC 1 cut(s) 513
Bso31I GGTCTC 1 cut(s) 982
BsoBI CYCGRG 1 cut(s) 310
Bsp1286I GDGCHC 1 cut(s) 981
Bsp143I GATC 4 cut(s) 373, 421, 715, 901
BspACI CCGC 3 cut(s) 430, 575, 933
BspANI GGCC 1 cut(s) 513
BspCNI CTCAG 2 cut(s) 212, 693
BspHI TCATGA 1 cut(s) 796
BspMAI CTGCAG 1 cut(s) 742
BspMI ACCTGC 1 cut(s) 314
BspPI GGATC 1 cut(s) 723
BspTNI GGTCTC 1 cut(s) 982
BsrDI GCAATG 3 cut(s) 295, 750, 912
BssMI GATC 4 cut(s) 373, 421, 715, 901
BssNI GRCGYC 1 cut(s) 1042
Bst2UI CCWGG 1 cut(s) 79
Bst4CI ACNGT 4 cut(s) 271, 464, 611, 765
BstACI GRCGYC 1 cut(s) 1042
BstC8I GCNNGC 2 cut(s) 106, 324
BstDEI CTNAG 3 cut(s) 199, 642, 701
BstENI CCTNNNNNAGG 1 cut(s) 310
BstF5I GGATG 2 cut(s) 134, 735
BstHHI GCGC 1 cut(s) 469
BstKTI GATC 4 cut(s) 376, 424, 718, 904
BstMAI GTCTC 2 cut(s) 27, 982
BstMBI GATC 4 cut(s) 373, 421, 715, 901
BstMWI GCNNNNNNNGC 2 cut(s) 194, 849
BstNI CCWGG 1 cut(s) 79
BstSCI CCNGG 2 cut(s) 77, 124
BstSFI CTRYAG 3 cut(s) 738, 818, 838
BstV1I GCAGC 3 cut(s) 172, 752, 864
BstV2I GAAGAC 1 cut(s) 70
BstX2I RGATCY 1 cut(s) 715
BstYI RGATCY 1 cut(s) 715
BsuI GTATCC 1 cut(s) 994
BsuRI GGCC 1 cut(s) 513
BtgZI GCGATG 1 cut(s) 377
BtsCI GGATG 2 cut(s) 134, 735
BtsI GCAGTG 1 cut(s) 637
BtsIMutI CAGTG 4 cut(s) 294, 561, 637, 867
BveI ACCTGC 1 cut(s) 314
Cac8I GCNNGC 2 cut(s) 106, 324
CciI TCATGA 1 cut(s) 796
CfoI GCGC 1 cut(s) 469
Cfr13I GGNCC 1 cut(s) 784
CseI GACGC 1 cut(s) 1050
CviAII CATG 3 cut(s) 522, 597, 797
CviJI RGCY 9 cut(s) 97, 104, 180, 513, 682, 705, 794, 843, 985
CviKI_1 RGCY 9 cut(s) 97, 104, 180, 513, 682, 705, 794, 843, 985
DdeI CTNAG 3 cut(s) 199, 642, 701
DpnI GATC 4 cut(s) 375, 423, 717, 903
DpnII GATC 4 cut(s) 373, 421, 715, 901
DraI TTTAAA 1 cut(s) 847
EciI GGCGGA 1 cut(s) 948
Eco31I GGTCTC 1 cut(s) 982
Eco47I GGWCC 1 cut(s) 784
Eco88I CYCGRG 1 cut(s) 310
EcoNI CCTNNNNNAGG 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 77
EcoT22I ATGCAT 1 cut(s) 1033
FaeI CATG 3 cut(s) 525, 600, 800
FalI AAGNNNNNCTT 4 cut(s) 702, 734, 771, 803
FatI CATG 3 cut(s) 521, 596, 796
FbaI TGATCA 1 cut(s) 373
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 3 cut(s) 186, 741, 853
FokI GGATG 2 cut(s) 141, 742
Fsp4HI GCNGC 3 cut(s) 186, 741, 853
FspBI CTAG 1 cut(s) 690
GlaI GCGC 1 cut(s) 468
GluI GCNGC 3 cut(s) 186, 741, 853
HaeIII GGCC 1 cut(s) 513
HapII CCGG 1 cut(s) 125
HgaI GACGC 1 cut(s) 1050
HhaI GCGC 1 cut(s) 469
Hin1I GRCGYC 1 cut(s) 1042
Hin1II CATG 3 cut(s) 525, 600, 800
Hin6I GCGC 1 cut(s) 467
HinP1I GCGC 1 cut(s) 467
HincII GTYRAC 1 cut(s) 494
HindII GTYRAC 1 cut(s) 494
HinfI GANTC 2 cut(s) 35, 388
HpaII CCGG 1 cut(s) 125
HphI GGTGA 3 cut(s) 26, 114, 142
Hpy166II GTNNAC 3 cut(s) 361, 494, 784
Hpy188I TCNGA 4 cut(s) 202, 369, 387, 702
Hpy188III TCNNGA 3 cut(s) 60, 797, 1035
Hpy8I GTNNAC 3 cut(s) 361, 494, 784
HpyAV CCTTC 1 cut(s) 23
HpyCH4III ACNGT 4 cut(s) 271, 464, 611, 765
HpyCH4V TGCA 7 cut(s) 108, 251, 275, 521, 740, 917, 1031
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 849
HpyF3I CTNAG 3 cut(s) 199, 642, 701
Hsp92I GRCGYC 1 cut(s) 1042
Hsp92II CATG 3 cut(s) 525, 600, 800
HspAI GCGC 1 cut(s) 467
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 4 cut(s) 373, 421, 715, 901
LmnI GCTCC 2 cut(s) 432, 674
Lsp1109I GCAGC 3 cut(s) 172, 752, 864
LweI GCATC 2 cut(s) 720, 1040
MaeI CTAG 1 cut(s) 690
MaeIII GTNAC 5 cut(s) 14, 120, 328, 416, 605
MalI GATC 4 cut(s) 375, 423, 717, 903
MboI GATC 4 cut(s) 373, 421, 715, 901
MboII GAAGA 1 cut(s) 75
MfeI CAATTG 2 cut(s) 174, 498
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 1 cut(s) 981
MluCI AATT 6 cut(s) 114, 174, 221, 498, 685, 1048
MlyI GAGTC 1 cut(s) 29
MmeI TCCRAC 1 cut(s) 863
Mph1103I ATGCAT 1 cut(s) 1033
MseI TTAA 4 cut(s) 405, 534, 846, 1054
MslI CAYNNNNRTG 2 cut(s) 127, 968
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 2 cut(s) 79, 126
MunI CAATTG 2 cut(s) 174, 498
MvaI CCWGG 1 cut(s) 79
MwoI GCNNNNNNNGC 2 cut(s) 194, 849
NciI CCSGG 1 cut(s) 126
NdeII GATC 4 cut(s) 373, 421, 715, 901
NlaIII CATG 3 cut(s) 525, 600, 800
NmeAIII GCCGAG 1 cut(s) 1011
NmuCI GTSAC 5 cut(s) 14, 120, 328, 416, 605
NsiI ATGCAT 1 cut(s) 1033
PaeR7I CTCGAG 1 cut(s) 310
PagI TCATGA 1 cut(s) 796
PfeI GAWTC 1 cut(s) 388
PkrI GCNGC 3 cut(s) 187, 742, 854
PleI GAGTC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 29
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PspPI GGNCC 1 cut(s) 784
PspXI VCTCGAGB 1 cut(s) 310
PstI CTGCAG 1 cut(s) 742
PsuI RGATCY 1 cut(s) 715
RseI CAYNNNNRTG 2 cut(s) 127, 968
SaqAI TTAA 4 cut(s) 405, 534, 846, 1054
SatI GCNGC 3 cut(s) 186, 741, 853
Sau3AI GATC 4 cut(s) 373, 421, 715, 901
Sau96I GGNCC 1 cut(s) 784
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 2 cut(s) 79, 126
SduI GDGCHC 1 cut(s) 981
SfaNI GCATC 2 cut(s) 720, 1040
SfcI CTRYAG 3 cut(s) 738, 818, 838
Sfr274I CTCGAG 1 cut(s) 310
SinI GGWCC 1 cut(s) 784
SlaI CTCGAG 1 cut(s) 310
SmiMI CAYNNNNRTG 2 cut(s) 127, 968
SmlI CTYRAG 3 cut(s) 310, 788, 980
SmoI CTYRAG 3 cut(s) 310, 788, 980
Sse9I AATT 6 cut(s) 114, 174, 221, 498, 685, 1048
SsiI CCGC 3 cut(s) 430, 575, 933
SspI AATATT 2 cut(s) 74, 144
SspMI CTAG 1 cut(s) 690
StyD4I CCNGG 2 cut(s) 77, 124
TaaI ACNGT 4 cut(s) 271, 464, 611, 765
TaqI TCGA 1 cut(s) 311
TasI AATT 6 cut(s) 114, 174, 221, 498, 685, 1048
TfiI GAWTC 1 cut(s) 388
Tru1I TTAA 4 cut(s) 405, 534, 846, 1054
Tru9I TTAA 4 cut(s) 405, 534, 846, 1054
TscAI CASTG 4 cut(s) 301, 561, 637, 867
TseFI GTSAC 5 cut(s) 14, 120, 328, 416, 605
TseI GCWGC 3 cut(s) 185, 740, 852
Tsp45I GTSAC 5 cut(s) 14, 120, 328, 416, 605
TspDTI ATGAA 4 cut(s) 136, 356, 816, 952
TspRI CASTG 4 cut(s) 301, 561, 637, 867
VpaK11BI GGWCC 1 cut(s) 784
XagI CCTNNNNNAGG 1 cut(s) 310
XapI RAATTY 1 cut(s) 1048
XhoI CTCGAG 1 cut(s) 310
XmiI GTMKAC 1 cut(s) 360
XspI CTAG 1 cut(s) 690
Zsp2I ATGCAT 1 cut(s) 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.