FvH4_2g18500

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002266
Physical Location & Seq
Forward (+)
15882337 .. 15885054
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g18500.t1

Sequence Viewer

Length: 1173 bp
ATGGATTTGGGATCAACCATGGTGAGCTTAAGAAGACAGAAACGCTTGAAAACATGTTCCGGAAGTAGTTCGGGTGCAGCTCCACTTCATGGTTTTTGTGACAAGGGAACTTCTCTTCAAACTTCCACCCAGGAGAAACCTGATGATGTACATCTCACGCCTTCAAACAATGTAAATCAATCAGAGATTATCAGTATATTGAGAGACCCAGCTGCTGCTGAGAGGGTTTTGCTGGACCTACTTGGTCCTGCTGATACCAGGATTATTCAGGAGTATATTGACGGAGATCTGAAGCAGAACTTAGCACACCATGCCCTTGCTTCCTGCCTCCATCTTGCTATGTGGCTAAGGGATACTGGAGATGTCTCCAAGGAGCGAGATGAAGCAAATGCTCGTGTTGCAGACCTGGAGAACAGAGTTAGAAACTTTGGGGAAACCACTACGAAACAGGAGGCAGTAATCGAGATTCTGAGGCAACAGATCCTGAAATACTCTAGCATGAAGAGGGAGTATGAATCCAAGCTTGATGGTTTGTTAAATGATGTTAGTCATCAGAGGCAGAAGGCTGAAATGTGGGAGATTACCCAGGGTCTTCATGTTGCTGGGAAAGGTATTGAGAAGATGACAAGTACCTTGTCTCAGGCTGAGGTCCAAGTGAAGCAGCTCCAGAAGAACTGTGACGTGTATAGGGCACTTTGCGACATGGAAATGGAAAAGCGTACCAAAGAAATGATTGCTCCAAGTGCTGAACTAAAAGTGGTTCTCCAGAGGAACGAAAACCATAAGCAGAACAATGATGGTAACATGATAACCAGGGAGATGATTAAAGAACTCAAAAGGCGGGTGAGGAGGTGGTCTCAAGAAAAGTTTCAGGAGGAAGGTGAAGACTGTGAAATGGATGGAGAGGGCTCTAATGCATCAAGGAAAGAAGTGGAGGCCGGGAATGCTCCGTGTTTCTGGACGATTTGCAATCACTGCAAAATGTACTTCGAGTATCATAGGATTTATCAAAGAAACCGTCTCCTTTGCCAAAACTGTCATAACACCTTTATGGCCTTAGAAGTTGCTCCACTGCCACAGATTTCCAGGTCATCCAGTAGCTCTTCTCTCCAGCAACATCAGAATTCAAGTGACCATGCTTCATTGAAGGTGAAAGGCTCTGCTGCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

391

Amino Acids

44.18

Weight (kDa)

6.9

Isoelectric Point (pI)

51.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_20 PF23551 319 - 352 6.9e-10 Zinc beta-ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 89
AccIII TCCGGA 1 cut(s) 59
AciI CCGC 1 cut(s) 841
AclWI GGATC 2 cut(s) 19, 475
AcsI RAATTY 1 cut(s) 1123
AcuI CTGAAG 1 cut(s) 311
AfaI GTAC 4 cut(s) 150, 631, 721, 986
AfiI CCNNNNNNNGG 1 cut(s) 89
AflII CTTAAG 1 cut(s) 28
AflIII ACRYGT 2 cut(s) 53, 681
AgsI TTSAA 5 cut(s) 49, 119, 165, 1128, 1147
AjiI CACGTC 1 cut(s) 682
AjnI CCWGG 6 cut(s) 129, 257, 405, 585, 812, 1085
AluBI AGCT 6 cut(s) 27, 80, 212, 523, 664, 1101
AluI AGCT 6 cut(s) 27, 80, 212, 523, 664, 1101
Alw26I GTCTC 5 cut(s) 198, 370, 642, 861, 1025
AlwI GGATC 2 cut(s) 19, 475
AlwNI CAGNNNCTG 2 cut(s) 215, 484
Aor13HI TCCGGA 1 cut(s) 59
AoxI GGCC 2 cut(s) 936, 1053
ApeKI GCWGC 6 cut(s) 77, 212, 215, 661, 1163, 1166
ApoI RAATTY 1 cut(s) 1123
ArsI GACNNNNNNTTYG 2 cut(s) 1003, 1035
Asp700I GAANNNNTTC 2 cut(s) 67, 867
AspS9I GGNCC 3 cut(s) 235, 245, 649
AsuC2I CCSGG 1 cut(s) 940
AsuHPI GGTGA 4 cut(s) 34, 856, 893, 1162
AvaII GGWCC 3 cut(s) 235, 245, 649
BaeGI GKGCMC 1 cut(s) 694
BanII GRGCYC 1 cut(s) 911
BauI CACGAG 1 cut(s) 393
BbsI GAAGAC 3 cut(s) 40, 584, 891
BbvCI CCTCAGC 1 cut(s) 645
BbvI GCAGC 6 cut(s) 89, 199, 202, 673, 1150, 1153
BccI CCATC 4 cut(s) 339, 521, 791, 893
BciT130I CCWGG 6 cut(s) 131, 259, 407, 587, 814, 1087
BciVI GTATCC 1 cut(s) 346
BcnI CCSGG 1 cut(s) 940
BcoDI GTCTC 5 cut(s) 198, 370, 642, 861, 1025
BfaI CTAG 1 cut(s) 495
BfrI CTTAAG 1 cut(s) 28
BfuI GTATCC 1 cut(s) 346
BglII AGATCT 1 cut(s) 286
BisI GCNGC 6 cut(s) 78, 213, 216, 662, 1164, 1167
BlsI GCNGC 6 cut(s) 79, 214, 217, 663, 1165, 1168
Bme1390I CCNGG 7 cut(s) 131, 259, 407, 587, 814, 940, 1087
Bme18I GGWCC 3 cut(s) 235, 245, 649
BmgBI CACGTC 1 cut(s) 682
BmgT120I GGNCC 3 cut(s) 235, 245, 649
BmrFI CCNGG 7 cut(s) 131, 259, 407, 587, 814, 940, 1087
BmsI GCATC 1 cut(s) 926
BpiI GAAGAC 3 cut(s) 40, 584, 891
BplI GAGNNNNNCTC 2 cut(s) 841, 873
BpmI CTGGAG 5 cut(s) 378, 428, 650, 749, 1094
Bpu10I CCTNAGC 2 cut(s) 347, 645
BpuEI CTTGAG 1 cut(s) 843
BpuMI CCSGG 1 cut(s) 940
BsaBI GATNNNNATC 1 cut(s) 150
BsaI GGTCTC 2 cut(s) 198, 861
BsaJI CCNNGG 6 cut(s) 18, 129, 369, 585, 586, 813
BsaWI WCCGGW 1 cut(s) 59
Bsc4I CCNNNNNNNGG 1 cut(s) 89
Bse1I ACTGG 2 cut(s) 361, 1095
Bse8I GATNNNNATC 1 cut(s) 150
BseAI TCCGGA 1 cut(s) 59
BseBI CCWGG 6 cut(s) 131, 259, 407, 587, 814, 1087
BseDI CCNNGG 6 cut(s) 18, 129, 369, 585, 586, 813
BseGI GGATG 2 cut(s) 904, 1091
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 89
BseMII CTCAG 4 cut(s) 210, 461, 636, 653
BseNI ACTGG 2 cut(s) 361, 1095
BseRI GAGGAG 1 cut(s) 862
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 6 cut(s) 89, 199, 202, 673, 1150, 1153
BseYI CCCAGC 2 cut(s) 208, 602
BsgI GTGCAG 1 cut(s) 96
BshFI GGCC 2 cut(s) 938, 1055
BsiSI CCGG 2 cut(s) 60, 939
BslI CCNNNNNNNGG 1 cut(s) 89
BsmAI GTCTC 5 cut(s) 198, 370, 642, 861, 1025
BsmBI CGTCTC 1 cut(s) 1025
BsmI GAATGC 1 cut(s) 949
BsnI GGCC 2 cut(s) 938, 1055
Bso31I GGTCTC 2 cut(s) 198, 861
Bsp1286I GDGCHC 2 cut(s) 694, 911
Bsp13I TCCGGA 1 cut(s) 59
Bsp1407I TGTACA 1 cut(s) 148
Bsp143I GATC 3 cut(s) 11, 286, 480
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 1 cut(s) 841
BspANI GGCC 2 cut(s) 938, 1055
BspCNI CTCAG 4 cut(s) 211, 462, 637, 652
BspEI TCCGGA 1 cut(s) 59
BspPI GGATC 2 cut(s) 19, 475
BspQI GCTCTTC 1 cut(s) 1108
BspTI CTTAAG 1 cut(s) 28
BspTNI GGTCTC 2 cut(s) 198, 861
BsrGI TGTACA 1 cut(s) 148
BsrI ACTGG 2 cut(s) 361, 1095
BssECI CCNNGG 6 cut(s) 18, 129, 369, 585, 586, 813
BssMI GATC 3 cut(s) 11, 286, 480
BssSI CACGAG 1 cut(s) 393
BssT1I CCWWGG 2 cut(s) 18, 369
Bst2BI CACGAG 1 cut(s) 393
Bst2UI CCWGG 6 cut(s) 131, 259, 407, 587, 814, 1087
Bst4CI ACNGT 4 cut(s) 677, 890, 1019, 1037
Bst6I CTCTTC 3 cut(s) 120, 497, 1108
BstAFI CTTAAG 1 cut(s) 28
BstAPI GCANNNNNTGC 2 cut(s) 311, 975
BstAUI TGTACA 1 cut(s) 148
BstDEI CTNAG 7 cut(s) 219, 301, 347, 470, 639, 645, 1057
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 2 cut(s) 904, 1091
BstKTI GATC 3 cut(s) 14, 289, 483
BstMAI GTCTC 5 cut(s) 198, 370, 642, 861, 1025
BstMBI GATC 3 cut(s) 11, 286, 480
BstMWI GCNNNNNNNGC 5 cut(s) 311, 398, 743, 944, 975
BstNI CCWGG 6 cut(s) 131, 259, 407, 587, 814, 1087
BstNSI RCATGY 1 cut(s) 57
BstSCI CCNGG 7 cut(s) 129, 257, 405, 585, 812, 938, 1085
BstSLI GKGCMC 1 cut(s) 694
BstV1I GCAGC 6 cut(s) 89, 199, 202, 673, 1150, 1153
BstV2I GAAGAC 3 cut(s) 40, 584, 891
BstX2I RGATCY 2 cut(s) 286, 480
BstYI RGATCY 2 cut(s) 286, 480
BsuI GTATCC 1 cut(s) 346
BsuRI GGCC 2 cut(s) 938, 1055
BtgI CCRYGG 1 cut(s) 18
BtrI CACGTC 1 cut(s) 682
BtsCI GGATG 2 cut(s) 904, 1091
BtsI GCAGTG 2 cut(s) 973, 1070
BtsIMutI CAGTG 2 cut(s) 973, 1070
CaiI CAGNNNCTG 2 cut(s) 215, 484
Cfr13I GGNCC 3 cut(s) 235, 245, 649
Csp6I GTAC 4 cut(s) 149, 630, 720, 985
CviAII CATG 9 cut(s) 19, 54, 89, 311, 499, 596, 703, 805, 1136
CviQI GTAC 4 cut(s) 149, 630, 720, 985
DdeI CTNAG 7 cut(s) 219, 301, 347, 470, 639, 645, 1057
DpnI GATC 3 cut(s) 13, 288, 482
DpnII GATC 3 cut(s) 11, 286, 480
Eam1104I CTCTTC 3 cut(s) 120, 497, 1108
EarI CTCTTC 3 cut(s) 120, 497, 1108
Eco130I CCWWGG 2 cut(s) 18, 369
Eco24I GRGCYC 1 cut(s) 911
Eco31I GGTCTC 2 cut(s) 198, 861
Eco47I GGWCC 3 cut(s) 235, 245, 649
Eco57I CTGAAG 1 cut(s) 311
EcoRI GAATTC 1 cut(s) 1123
EcoRII CCWGG 6 cut(s) 129, 257, 405, 585, 812, 1085
EcoT14I CCWWGG 2 cut(s) 18, 369
EcoT22I ATGCAT 1 cut(s) 919
EcoT38I GRGCYC 1 cut(s) 911
ErhI CCWWGG 2 cut(s) 18, 369
Esp3I CGTCTC 1 cut(s) 1025
FaeI CATG 9 cut(s) 22, 57, 92, 314, 502, 599, 706, 808, 1139
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FatI CATG 9 cut(s) 18, 53, 88, 310, 498, 595, 702, 804, 1135
FauI CCCGC 1 cut(s) 834
Fnu4HI GCNGC 6 cut(s) 78, 213, 216, 662, 1164, 1167
FokI GGATG 2 cut(s) 911, 1078
FriOI GRGCYC 1 cut(s) 911
Fsp4HI GCNGC 6 cut(s) 78, 213, 216, 662, 1164, 1167
FspBI CTAG 1 cut(s) 495
GluI GCNGC 6 cut(s) 78, 213, 216, 662, 1164, 1167
GsaI CCCAGC 2 cut(s) 212, 606
GsuI CTGGAG 5 cut(s) 378, 428, 650, 749, 1094
HaeIII GGCC 2 cut(s) 938, 1055
HapII CCGG 2 cut(s) 60, 939
Hin1II CATG 9 cut(s) 22, 57, 92, 314, 502, 599, 706, 808, 1139
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 2 cut(s) 466, 515
HpaII CCGG 2 cut(s) 60, 939
HphI GGTGA 4 cut(s) 34, 856, 893, 1162
Hpy188I TCNGA 5 cut(s) 184, 291, 471, 555, 1122
Hpy188III TCNNGA 9 cut(s) 60, 269, 463, 484, 667, 766, 860, 872, 958
HpyAV CCTTC 4 cut(s) 171, 556, 872, 1141
HpyCH4III ACNGT 4 cut(s) 677, 890, 1019, 1037
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 6 cut(s) 77, 401, 917, 969, 978, 1169
HpyF10VI GCNNNNNNNGC 5 cut(s) 311, 398, 743, 944, 975
HpyF3I CTNAG 7 cut(s) 219, 301, 347, 470, 639, 645, 1057
HpySE526I ACGT 1 cut(s) 681
Hsp92II CATG 9 cut(s) 22, 57, 92, 314, 502, 599, 706, 808, 1139
Kpn2I TCCGGA 1 cut(s) 59
Kzo9I GATC 3 cut(s) 11, 286, 480
LguI GCTCTTC 1 cut(s) 1108
LmnI GCTCC 6 cut(s) 85, 373, 669, 742, 952, 1072
Lsp1109I GCAGC 6 cut(s) 89, 199, 202, 673, 1150, 1153
LweI GCATC 1 cut(s) 926
MaeI CTAG 1 cut(s) 495
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 4 cut(s) 98, 677, 800, 1130
MalI GATC 3 cut(s) 13, 288, 482
MboI GATC 3 cut(s) 11, 286, 480
MboII GAAGA 8 cut(s) 45, 107, 514, 584, 631, 682, 896, 1095
MflI RGATCY 2 cut(s) 286, 480
MhlI GDGCHC 2 cut(s) 694, 911
MluCI AATT 1 cut(s) 1123
Mph1103I ATGCAT 1 cut(s) 919
MroI TCCGGA 1 cut(s) 59
MroXI GAANNNNTTC 2 cut(s) 67, 867
MseI TTAA 3 cut(s) 29, 536, 825
MslI CAYNNNNRTG 2 cut(s) 707, 1049
MspA1I CMGCKG 1 cut(s) 212
MspCI CTTAAG 1 cut(s) 28
MspI CCGG 2 cut(s) 60, 939
MspR9I CCNGG 7 cut(s) 131, 259, 407, 587, 814, 940, 1087
Mva1269I GAATGC 1 cut(s) 949
MvaI CCWGG 6 cut(s) 131, 259, 407, 587, 814, 1087
MwoI GCNNNNNNNGC 5 cut(s) 311, 398, 743, 944, 975
NciI CCSGG 1 cut(s) 940
NcoI CCATGG 1 cut(s) 18
NdeII GATC 3 cut(s) 11, 286, 480
NlaIII CATG 9 cut(s) 22, 57, 92, 314, 502, 599, 706, 808, 1139
NmuCI GTSAC 3 cut(s) 98, 677, 1130
NsiI ATGCAT 1 cut(s) 919
NspI RCATGY 1 cut(s) 57
PasI CCCWGGG 1 cut(s) 586
PciI ACATGT 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 1108
PctI GAATGC 1 cut(s) 949
PdmI GAANNNNTTC 2 cut(s) 67, 867
PfeI GAWTC 2 cut(s) 466, 515
PflMI CCANNNNNTGG 1 cut(s) 89
PkrI GCNGC 6 cut(s) 79, 214, 217, 663, 1165, 1168
PscI ACATGT 1 cut(s) 53
Psp6I CCWGG 6 cut(s) 129, 257, 405, 585, 812, 1085
PspFI CCCAGC 2 cut(s) 208, 602
PspGI CCWGG 6 cut(s) 129, 257, 405, 585, 812, 1085
PspPI GGNCC 3 cut(s) 235, 245, 649
PstNI CAGNNNCTG 2 cut(s) 215, 484
PsuI RGATCY 2 cut(s) 286, 480
PvuII CAGCTG 1 cut(s) 212
RsaI GTAC 4 cut(s) 150, 631, 721, 986
RsaNI GTAC 4 cut(s) 149, 630, 720, 985
RseI CAYNNNNRTG 2 cut(s) 707, 1049
SapI GCTCTTC 1 cut(s) 1108
SaqAI TTAA 3 cut(s) 29, 536, 825
SatI GCNGC 6 cut(s) 78, 213, 216, 662, 1164, 1167
Sau3AI GATC 3 cut(s) 11, 286, 480
Sau96I GGNCC 3 cut(s) 235, 245, 649
ScrFI CCNGG 7 cut(s) 131, 259, 407, 587, 814, 940, 1087
SduI GDGCHC 2 cut(s) 694, 911
SfaNI GCATC 1 cut(s) 926
SinI GGWCC 3 cut(s) 235, 245, 649
SmiMI CAYNNNNRTG 2 cut(s) 707, 1049
SmlI CTYRAG 2 cut(s) 28, 858
SmoI CTYRAG 2 cut(s) 28, 858
Sse9I AATT 1 cut(s) 1123
SsiI CCGC 1 cut(s) 841
SspMI CTAG 1 cut(s) 495
StyD4I CCNGG 7 cut(s) 129, 257, 405, 585, 812, 938, 1085
StyI CCWWGG 2 cut(s) 18, 369
TaaI ACNGT 4 cut(s) 677, 890, 1019, 1037
TaiI ACGT 1 cut(s) 684
TaqI TCGA 2 cut(s) 462, 990
TasI AATT 1 cut(s) 1123
TatI WGTACW 2 cut(s) 148, 984
TfiI GAWTC 2 cut(s) 466, 515
Tru1I TTAA 3 cut(s) 29, 536, 825
Tru9I TTAA 3 cut(s) 29, 536, 825
TscAI CASTG 2 cut(s) 980, 1077
TseFI GTSAC 3 cut(s) 98, 677, 1130
TseI GCWGC 6 cut(s) 77, 212, 215, 661, 1163, 1166
Tsp45I GTSAC 3 cut(s) 98, 677, 1130
TspDTI ATGAA 6 cut(s) 77, 396, 515, 528, 584, 1131
TspGWI ACGGA 2 cut(s) 297, 939
TspRI CASTG 2 cut(s) 980, 1077
Van91I CCANNNNNTGG 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 28
VpaK11BI GGWCC 3 cut(s) 235, 245, 649
XapI RAATTY 1 cut(s) 1123
XceI RCATGY 1 cut(s) 57
XmnI GAANNNNTTC 2 cut(s) 67, 867
XspI CTAG 1 cut(s) 495
Zsp2I ATGCAT 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.