Rorug06G0159500

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002266
Physical Location & Seq
Forward (+)
22739205 .. 22743334
4130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0159500.1

Sequence Viewer

Length: 900 bp
ATGGCCACCGCGAGAGTGCTCGCCGTCTCCAACAGTTCGTGCCTGCTGACCAAGACGACGTCGTTCTTGGCGAAGCAGAGGCCGTACAGGAACAATCTCCGCTTCACTACTACTAGAAGAGACGCGGCAGCGCCGTCATTGTCGTCGCGGAGGAGGTCCTTAACTTGCAGGGCTATCTACAACCCCGAGGTTCAGATCAAGGAGGAAGGCCTCCCCGAAACCCTAGACTACCGCGTCTTCCTCGTTGACCGCTCCGGCCAGAAGGTTTCACCTTGGCATGATGTACCTCTGCGCTCGGGCGATGGTGCATTCCACTTTATTGTTGAGATACCCAAAGAATCGAGTGCAAAGATGGAGGTTGCTACTGATGAGCCAAACACTCCCATTAAGCAGGATACAAAGAAAGGAAAACTTCGTTACTACCCCTACAATATAAACTGGAATTATGGATTGCTTCCACAAACATGGGAAGACCCATCCCTTGCCAACTCTGAAGTGGATGGAGCATTTGGTGATAATGATCCAGTTGATGTTGTTGAGATTGGCGAAAGTCGGAGAAAGGTTGGCGAAATTCTCAAAGTAAAACCCTTGGCTGCTTTAGCTATGATTGATGAAGGGGAACTTGACTGGAAAATAGTTGCAATTTCATTGGATGATCCAAAATCTTCTCTGGTTAATGACATTGATGACGTTGAGAAGCATTTCCCGGGGACTCTCACTGCAATAAGGGACTGGTTTAGAGACTACAAGATCCCTGATGGAAAGCCTGCTAACAAGTTTGGTCTTGGAAACAAGCCAGCCAACAAGGATTATGCTCTCAAGGTCATAACTGAAACCAATGAATCATGGGCTAAACTCGTCAAGAGATCAATTCCTGCTGGAGATCTGTCCCTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

299

Amino Acids

33.49

Weight (kDa)

7.68

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 106 - 283 7.3e-41 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AatII GACGTC 1 cut(s) 62
AccBSI CCGCTC 1 cut(s) 252
AccII CGCG 4 cut(s) 11, 125, 148, 234
AciI CCGC 6 cut(s) 9, 100, 125, 148, 232, 250
AclWI GGATC 3 cut(s) 515, 650, 745
AcoI YGGCCR 2 cut(s) 3, 256
AcsI RAATTY 1 cut(s) 570
AcuI CTGAAG 1 cut(s) 513
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 2 cut(s) 86, 285
AluBI AGCT 1 cut(s) 602
AluI AGCT 1 cut(s) 602
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 3 cut(s) 31, 114, 735
AlwI GGATC 3 cut(s) 515, 650, 745
Ama87I CYCGRG 3 cut(s) 185, 295, 706
AoxI GGCC 4 cut(s) 3, 80, 208, 256
ApeKI GCWGC 2 cut(s) 128, 593
ApoI RAATTY 1 cut(s) 570
Asp700I GAANNNNTTC 1 cut(s) 701
AspLEI GCGC 2 cut(s) 133, 294
AspS9I GGNCC 1 cut(s) 156
AsuC2I CCSGG 2 cut(s) 707, 708
AsuHPI GGTGA 2 cut(s) 261, 524
AvaI CYCGRG 3 cut(s) 185, 295, 706
AvaII GGWCC 1 cut(s) 156
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 229, 477
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 2 cut(s) 140, 580
BccI CCATC 5 cut(s) 296, 346, 484, 494, 752
BceAI ACGGC 3 cut(s) 8, 67, 118
BciVI GTATCC 1 cut(s) 388
BcnI CCSGG 2 cut(s) 707, 708
BcoDI GTCTC 3 cut(s) 31, 114, 735
BfaI CTAG 3 cut(s) 114, 224, 898
BfoI RGCGCY 1 cut(s) 134
BfuI GTATCC 1 cut(s) 388
BglII AGATCT 1 cut(s) 883
BisI GCNGC 3 cut(s) 126, 129, 594
BlsI GCNGC 3 cut(s) 127, 130, 595
Bme1390I CCNGG 2 cut(s) 707, 708
Bme18I GGWCC 1 cut(s) 156
BmeT110I CYCGRG 3 cut(s) 185, 295, 706
BmgT120I GGNCC 1 cut(s) 156
BmrFI CCNGG 2 cut(s) 707, 708
BpiI GAAGAC 2 cut(s) 229, 477
BpmI CTGGAG 1 cut(s) 900
BpuEI CTTGAG 1 cut(s) 803
BpuMI CCSGG 2 cut(s) 707, 708
BsaBI GATNNNNATC 1 cut(s) 519
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 5 cut(s) 186, 272, 588, 706, 707
Bse1I ACTGG 4 cut(s) 443, 524, 632, 737
Bse8I GATNNNNATC 1 cut(s) 519
BseDI CCNNGG 5 cut(s) 186, 272, 588, 706, 707
BseGI GGATG 3 cut(s) 476, 505, 658
BseJI GATNNNNATC 1 cut(s) 519
BseNI ACTGG 4 cut(s) 443, 524, 632, 737
BseRI GAGGAG 1 cut(s) 166
BseXI GCAGC 2 cut(s) 140, 580
Bsh1236I CGCG 4 cut(s) 11, 125, 148, 234
BshFI GGCC 4 cut(s) 5, 82, 210, 258
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 3 cut(s) 185, 295, 706
BsiSI CCGG 2 cut(s) 255, 707
BslFI GGGAC 3 cut(s) 724, 743, 874
BsmAI GTCTC 3 cut(s) 31, 114, 735
BsmBI CGTCTC 2 cut(s) 31, 114
BsmFI GGGAC 3 cut(s) 724, 743, 874
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 4 cut(s) 5, 82, 210, 258
BsoBI CYCGRG 3 cut(s) 185, 295, 706
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 6 cut(s) 195, 520, 655, 750, 866, 883
BspACI CCGC 6 cut(s) 9, 100, 125, 148, 232, 250
BspANI GGCC 4 cut(s) 5, 82, 210, 258
BspFNI CGCG 4 cut(s) 11, 125, 148, 234
BspPI GGATC 3 cut(s) 515, 650, 745
BsrBI CCGCTC 1 cut(s) 252
BsrI ACTGG 4 cut(s) 443, 524, 632, 737
BssECI CCNNGG 5 cut(s) 186, 272, 588, 706, 707
BssMI GATC 6 cut(s) 195, 520, 655, 750, 866, 883
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 2 cut(s) 272, 588
Bst4CI ACNGT 1 cut(s) 35
Bst6I CTCTTC 1 cut(s) 112
BstACI GRCGYC 1 cut(s) 59
BstC8I GCNNGC 4 cut(s) 21, 44, 768, 798
BstF5I GGATG 3 cut(s) 476, 505, 658
BstFNI CGCG 4 cut(s) 11, 125, 148, 234
BstH2I RGCGCY 1 cut(s) 134
BstHHI GCGC 2 cut(s) 133, 294
BstKTI GATC 6 cut(s) 198, 523, 658, 753, 869, 886
BstMAI GTCTC 3 cut(s) 31, 114, 735
BstMBI GATC 6 cut(s) 195, 520, 655, 750, 866, 883
BstMWI GCNNNNNNNGC 1 cut(s) 599
BstSCI CCNGG 2 cut(s) 705, 706
BstUI CGCG 4 cut(s) 11, 125, 148, 234
BstV1I GCAGC 2 cut(s) 140, 580
BstV2I GAAGAC 2 cut(s) 229, 477
BstX2I RGATCY 2 cut(s) 750, 883
BstXI CCANNNNNNTGG 1 cut(s) 465
BstYI RGATCY 2 cut(s) 750, 883
BsuI GTATCC 1 cut(s) 388
BsuRI GGCC 4 cut(s) 5, 82, 210, 258
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 3 cut(s) 476, 505, 658
BtsI GCAGTG 1 cut(s) 717
BtsIMutI CAGTG 1 cut(s) 717
Cac8I GCNNGC 4 cut(s) 21, 44, 768, 798
CfoI GCGC 2 cut(s) 133, 294
Cfr13I GGNCC 1 cut(s) 156
Cfr9I CCCGGG 1 cut(s) 706
CseI GACGC 2 cut(s) 131, 223
Csp6I GTAC 2 cut(s) 85, 284
CviAII CATG 3 cut(s) 278, 465, 846
CviQI GTAC 2 cut(s) 85, 284
DpnI GATC 6 cut(s) 197, 522, 657, 752, 868, 885
DpnII GATC 6 cut(s) 195, 520, 655, 750, 866, 883
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 2 cut(s) 3, 256
Eam1104I CTCTTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 112
Eco130I CCWWGG 2 cut(s) 272, 588
Eco147I AGGCCT 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 156
Eco57I CTGAAG 1 cut(s) 513
Eco88I CYCGRG 3 cut(s) 185, 295, 706
EcoO109I RGGNCCY 1 cut(s) 156
EcoT14I CCWWGG 2 cut(s) 272, 588
ErhI CCWWGG 2 cut(s) 272, 588
Esp3I CGTCTC 2 cut(s) 31, 114
FaeI CATG 3 cut(s) 281, 468, 849
FaiI YATR 8 cut(s) 279, 434, 447, 466, 605, 813, 827, 847
FalI AAGNNNNNCTT 4 cut(s) 396, 428, 606, 638
FaqI GGGAC 3 cut(s) 724, 743, 874
FatI CATG 3 cut(s) 277, 464, 845
Fnu4HI GCNGC 3 cut(s) 126, 129, 594
FokI GGATG 3 cut(s) 463, 512, 665
Fsp4HI GCNGC 3 cut(s) 126, 129, 594
FspBI CTAG 3 cut(s) 114, 224, 898
GlaI GCGC 2 cut(s) 132, 293
GluI GCNGC 3 cut(s) 126, 129, 594
GsuI CTGGAG 1 cut(s) 900
HaeII RGCGCY 1 cut(s) 134
HaeIII GGCC 4 cut(s) 5, 82, 210, 258
HapII CCGG 2 cut(s) 255, 707
HgaI GACGC 2 cut(s) 131, 223
HhaI GCGC 2 cut(s) 133, 294
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 3 cut(s) 281, 468, 849
Hin6I GCGC 2 cut(s) 131, 292
HinP1I GCGC 2 cut(s) 131, 292
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 3 cut(s) 338, 712, 842
HpaII CCGG 2 cut(s) 255, 707
HphI GGTGA 2 cut(s) 261, 524
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 3 cut(s) 195, 493, 555
Hpy188III TCNNGA 1 cut(s) 862
Hpy8I GTNNAC 1 cut(s) 247
Hpy99I CGWCG 3 cut(s) 61, 64, 148
HpyAV CCTTC 3 cut(s) 200, 256, 608
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4IV ACGT 2 cut(s) 59, 690
HpyCH4V TGCA 5 cut(s) 168, 308, 347, 641, 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 599
HpySE526I ACGT 2 cut(s) 59, 690
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 3 cut(s) 281, 468, 849
HspAI GCGC 2 cut(s) 131, 292
Kzo9I GATC 6 cut(s) 195, 520, 655, 750, 866, 883
LmnI GCTCC 2 cut(s) 257, 503
Lsp1109I GCAGC 2 cut(s) 140, 580
MaeI CTAG 3 cut(s) 114, 224, 898
MaeII ACGT 2 cut(s) 59, 690
MaeIII GTNAC 1 cut(s) 416
MalI GATC 6 cut(s) 197, 522, 657, 752, 868, 885
MbiI CCGCTC 1 cut(s) 252
MboI GATC 6 cut(s) 195, 520, 655, 750, 866, 883
MboII GAAGA 4 cut(s) 129, 229, 482, 657
MflI RGATCY 2 cut(s) 750, 883
MhlI GDGCHC 1 cut(s) 21
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 442, 570, 642, 870
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 706
MmeI TCCRAC 2 cut(s) 54, 533
MnlI CCTC 9 cut(s) 72, 144, 147, 181, 196, 221, 251, 297, 349
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 701
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 161, 387, 675
MslI CAYNNNNRTG 1 cut(s) 463
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 255, 707
MspR9I CCNGG 2 cut(s) 707, 708
Mva1269I GAATGC 1 cut(s) 308
MvnI CGCG 4 cut(s) 11, 125, 148, 234
MwoI GCNNNNNNNGC 1 cut(s) 599
NciI CCSGG 2 cut(s) 707, 708
NdeII GATC 6 cut(s) 195, 520, 655, 750, 866, 883
NlaIII CATG 3 cut(s) 281, 468, 849
PceI AGGCCT 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PctI GAATGC 1 cut(s) 308
PdmI GAANNNNTTC 1 cut(s) 701
PfeI GAWTC 2 cut(s) 338, 842
PflFI GACNNNGTC 1 cut(s) 58
PkrI GCNGC 3 cut(s) 127, 130, 595
PleI GAGTC 1 cut(s) 706
PpsI GAGTC 1 cut(s) 706
PpuMI RGGWCCY 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 156
PspPI GGNCC 1 cut(s) 156
PspPPI RGGWCCY 1 cut(s) 156
PsuI RGATCY 2 cut(s) 750, 883
PsyI GACNNNGTC 1 cut(s) 58
RsaI GTAC 2 cut(s) 86, 285
RsaNI GTAC 2 cut(s) 85, 284
RseI CAYNNNNRTG 1 cut(s) 463
SaqAI TTAA 3 cut(s) 161, 387, 675
SatI GCNGC 3 cut(s) 126, 129, 594
Sau3AI GATC 6 cut(s) 195, 520, 655, 750, 866, 883
Sau96I GGNCC 1 cut(s) 156
SchI GAGTC 1 cut(s) 706
ScrFI CCNGG 2 cut(s) 707, 708
SduI GDGCHC 1 cut(s) 21
SinI GGWCC 1 cut(s) 156
SmaI CCCGGG 1 cut(s) 708
SmiMI CAYNNNNRTG 1 cut(s) 463
SmlI CTYRAG 1 cut(s) 818
SmoI CTYRAG 1 cut(s) 818
Sse9I AATT 4 cut(s) 442, 570, 642, 870
SseBI AGGCCT 1 cut(s) 210
SsiI CCGC 6 cut(s) 9, 100, 125, 148, 232, 250
SspMI CTAG 3 cut(s) 114, 224, 898
StuI AGGCCT 1 cut(s) 210
StyD4I CCNGG 2 cut(s) 705, 706
StyI CCWWGG 2 cut(s) 272, 588
TaaI ACNGT 1 cut(s) 35
TaiI ACGT 2 cut(s) 62, 693
TaqI TCGA 1 cut(s) 341
TasI AATT 4 cut(s) 442, 570, 642, 870
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 2 cut(s) 338, 842
Tru1I TTAA 3 cut(s) 161, 387, 675
Tru9I TTAA 3 cut(s) 161, 387, 675
TscAI CASTG 1 cut(s) 724
TseI GCWGC 2 cut(s) 128, 593
TspDTI ATGAA 3 cut(s) 627, 636, 855
TspMI CCCGGG 1 cut(s) 706
TspRI CASTG 1 cut(s) 724
Tth111I GACNNNGTC 1 cut(s) 58
VpaK11BI GGWCC 1 cut(s) 156
XapI RAATTY 1 cut(s) 570
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XmaI CCCGGG 1 cut(s) 706
XmnI GAANNNNTTC 1 cut(s) 701
XspI CTAG 3 cut(s) 114, 224, 898
ZraI GACGTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.