Rh6DG268200

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002266
Physical Location & Seq
Forward (+)
46106422 .. 46110283
3862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG268200.1

Sequence Viewer

Length: 606 bp
ATGTGGCTGAGGAAAACTGGAGATGTTTCGAGGCAGCGTGATGAAGCCATAGCTCGTGTTGCAGAGCTGGAGGAAAGAGTTAGGAGGTTTGAAAAGCTCTTTGTGAAACAGGAGGCTGAAAATGAGATTCTGAGCCTAGAGGTCTACGAGTACTGTAACATGAAGGAGGAGTACGAGTCTAAGATTAAGAGTCTATCAAATGAGGTTAACCAGCAGAAGCATGTGACTGTGGTCTTGGAGAGTAAGTTCAAGGATATGGAGGCCATGCACCAGGAGAAGGCTACTCGTCTGGAAAAGGCCTTGAAGCAGCTTAACGACGCCAACATGCTGCTTGCTGAGGTGAGTGAAGGTCTTGGTGTGGCCAGGGAACCAGGGGCCAGGGATCCTAGACATTTCTGGACGGTTTGCATTCACTGCAAAGTCCAGTATCAGTATATCAGGATCTATCAGAATCGCAGGCTTCGCTGCCCCAAATGTCGAAATGCTTTTCTTGCTATAGAAACTGTTTCATCTCCAAAGTCCTATCCATGGAGTAGCCCCTCATGGGTGAAGACTGAAGAAGATGGACAGGCTGCAGTCAAAGTGGAGGCTCAGGATGCTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

201

Amino Acids

23.48

Weight (kDa)

7.62

Isoelectric Point (pI)

46.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_20 PF23551 132 - 166 9.3e-14 Zinc beta-ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 144
AclWI GGATC 3 cut(s) 377, 390, 449
AcoI YGGCCR 1 cut(s) 360
AcuI CTGAAG 1 cut(s) 576
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 2 cut(s) 152, 173
AfiI CCNNNNNNNGG 3 cut(s) 277, 528, 544
AgsI TTSAA 3 cut(s) 92, 250, 304
AjnI CCWGG 4 cut(s) 270, 362, 370, 377
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
AluBI AGCT 4 cut(s) 53, 67, 97, 310
AluI AGCT 4 cut(s) 53, 67, 97, 310
AlwI GGATC 3 cut(s) 377, 390, 449
AoxI GGCC 4 cut(s) 261, 297, 360, 375
ApeKI GCWGC 5 cut(s) 34, 307, 328, 465, 572
AspS9I GGNCC 1 cut(s) 375
AsuHPI GGTGA 2 cut(s) 352, 559
BalI TGGCCA 1 cut(s) 362
BamHI GGATCC 1 cut(s) 382
BarI GAAGNNNNNNTAC 2 cut(s) 155, 187
BauI CACGAG 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 557
BbvCI CCTCAGC 2 cut(s) 8, 336
BbvI GCAGC 5 cut(s) 46, 315, 319, 452, 559
BccI CCATC 1 cut(s) 557
BciT130I CCWGG 4 cut(s) 272, 364, 372, 379
BfaI CTAG 2 cut(s) 137, 387
BfmI CTRYAG 2 cut(s) 495, 573
BisI GCNGC 5 cut(s) 35, 308, 329, 466, 573
BlsI GCNGC 5 cut(s) 36, 309, 330, 467, 574
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 4 cut(s) 272, 364, 372, 379
BmgT120I GGNCC 1 cut(s) 375
BmiI GGNNCC 3 cut(s) 369, 376, 384
BmrFI CCNGG 4 cut(s) 272, 364, 372, 379
BmsI GCATC 1 cut(s) 586
BoxI GACNNNNGTC 1 cut(s) 230
BpiI GAAGAC 1 cut(s) 557
BpmI CTGGAG 2 cut(s) 39, 89
Bpu10I CCTNAGC 3 cut(s) 8, 336, 591
BsaHI GRCGYC 1 cut(s) 318
BsaJI CCNNGG 4 cut(s) 363, 371, 378, 527
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 230, 260
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 528, 544
Bse1I ACTGG 2 cut(s) 22, 424
BseBI CCWGG 4 cut(s) 272, 364, 372, 379
BseDI CCNNGG 4 cut(s) 363, 371, 378, 527
BseGI GGATG 1 cut(s) 601
BseLI CCNNNNNNNGG 3 cut(s) 277, 528, 544
BseMII CTCAG 3 cut(s) 122, 327, 605
BseNI ACTGG 2 cut(s) 22, 424
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 5 cut(s) 46, 315, 319, 452, 559
BshFI GGCC 4 cut(s) 263, 299, 362, 377
BslI CCNNNNNNNGG 3 cut(s) 277, 528, 544
BsmI GAATGC 1 cut(s) 408
BsnI GGCC 4 cut(s) 263, 299, 362, 377
Bsp143I GATC 2 cut(s) 382, 441
Bsp19I CCATGG 1 cut(s) 527
BspANI GGCC 4 cut(s) 263, 299, 362, 377
BspCNI CTCAG 3 cut(s) 123, 328, 604
BspLI GGNNCC 3 cut(s) 369, 376, 384
BspMAI CTGCAG 1 cut(s) 577
BspPI GGATC 3 cut(s) 377, 390, 449
BsrI ACTGG 2 cut(s) 22, 424
BssECI CCNNGG 4 cut(s) 363, 371, 378, 527
BssMI GATC 2 cut(s) 382, 441
BssNI GRCGYC 1 cut(s) 318
BssSI CACGAG 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 527
Bst2BI CACGAG 1 cut(s) 54
Bst2UI CCWGG 4 cut(s) 272, 364, 372, 379
Bst4CI ACNGT 4 cut(s) 155, 229, 403, 505
BstACI GRCGYC 1 cut(s) 318
BstAPI GCANNNNNTGC 1 cut(s) 414
BstC8I GCNNGC 2 cut(s) 333, 458
BstDEI CTNAG 5 cut(s) 8, 131, 180, 336, 591
BstDSI CCRYGG 1 cut(s) 527
BstF5I GGATG 1 cut(s) 601
BstKTI GATC 2 cut(s) 385, 444
BstMBI GATC 2 cut(s) 382, 441
BstMWI GCNNNNNNNGC 5 cut(s) 59, 414, 462, 491, 596
BstNI CCWGG 4 cut(s) 272, 364, 372, 379
BstNSI RCATGY 2 cut(s) 224, 328
BstPAI GACNNNNGTC 1 cut(s) 230
BstSCI CCNGG 4 cut(s) 270, 362, 370, 377
BstSFI CTRYAG 2 cut(s) 495, 573
BstV1I GCAGC 5 cut(s) 46, 315, 319, 452, 559
BstV2I GAAGAC 1 cut(s) 557
BstX2I RGATCY 2 cut(s) 382, 441
BstYI RGATCY 2 cut(s) 382, 441
BsuRI GGCC 4 cut(s) 263, 299, 362, 377
BtgI CCRYGG 1 cut(s) 527
BtsCI GGATG 1 cut(s) 601
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 412
Cac8I GCNNGC 2 cut(s) 333, 458
Cfr13I GGNCC 1 cut(s) 375
CseI GACGC 1 cut(s) 326
Csp6I GTAC 2 cut(s) 151, 172
CviAII CATG 6 cut(s) 160, 221, 265, 325, 528, 543
CviQI GTAC 2 cut(s) 151, 172
DdeI CTNAG 5 cut(s) 8, 131, 180, 336, 591
DpnI GATC 2 cut(s) 384, 443
DpnII GATC 2 cut(s) 382, 441
EaeI YGGCCR 1 cut(s) 360
Eco130I CCWWGG 1 cut(s) 527
Eco147I AGGCCT 1 cut(s) 299
Eco57I CTGAAG 1 cut(s) 576
EcoRII CCWGG 4 cut(s) 270, 362, 370, 377
EcoT14I CCWWGG 1 cut(s) 527
ErhI CCWWGG 1 cut(s) 527
FaeI CATG 6 cut(s) 163, 224, 268, 328, 531, 546
FatI CATG 6 cut(s) 159, 220, 264, 324, 527, 542
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 5 cut(s) 35, 308, 329, 466, 573
Fsp4HI GCNGC 5 cut(s) 35, 308, 329, 466, 573
FspBI CTAG 2 cut(s) 137, 387
GluI GCNGC 5 cut(s) 35, 308, 329, 466, 573
GsuI CTGGAG 2 cut(s) 39, 89
HaeIII GGCC 4 cut(s) 263, 299, 362, 377
HgaI GACGC 1 cut(s) 326
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 6 cut(s) 163, 224, 268, 328, 531, 546
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 4 cut(s) 127, 176, 190, 451
HpaI GTTAAC 1 cut(s) 208
HphI GGTGA 2 cut(s) 352, 559
Hpy166II GTNNAC 2 cut(s) 145, 208
Hpy188I TCNGA 2 cut(s) 132, 450
Hpy188III TCNNGA 4 cut(s) 290, 397, 439, 593
Hpy8I GTNNAC 2 cut(s) 145, 208
Hpy99I CGWCG 1 cut(s) 320
HpyAV CCTTC 3 cut(s) 157, 271, 341
HpyCH4III ACNGT 4 cut(s) 155, 229, 403, 505
HpyCH4V TGCA 5 cut(s) 62, 268, 408, 417, 575
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 414, 462, 491, 596
HpyF3I CTNAG 5 cut(s) 8, 131, 180, 336, 591
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 6 cut(s) 163, 224, 268, 328, 531, 546
KspAI GTTAAC 1 cut(s) 208
Kzo9I GATC 2 cut(s) 382, 441
LmnI GCTCC 1 cut(s) 604
Lsp1109I GCAGC 5 cut(s) 46, 315, 319, 452, 559
LweI GCATC 1 cut(s) 586
MaeI CTAG 2 cut(s) 137, 387
MaeIII GTNAC 2 cut(s) 155, 223
MalI GATC 2 cut(s) 384, 443
MboI GATC 2 cut(s) 382, 441
MboII GAAGA 3 cut(s) 562, 569, 572
MflI RGATCY 2 cut(s) 382, 441
MlsI TGGCCA 1 cut(s) 362
MluNI TGGCCA 1 cut(s) 362
MlyI GAGTC 2 cut(s) 185, 199
Mox20I TGGCCA 1 cut(s) 362
MscI TGGCCA 1 cut(s) 362
MseI TTAA 3 cut(s) 186, 207, 312
Msp20I TGGCCA 1 cut(s) 362
MspR9I CCNGG 4 cut(s) 272, 364, 372, 379
Mva1269I GAATGC 1 cut(s) 408
MvaI CCWGG 4 cut(s) 272, 364, 372, 379
MwoI GCNNNNNNNGC 5 cut(s) 59, 414, 462, 491, 596
NcoI CCATGG 1 cut(s) 527
NdeII GATC 2 cut(s) 382, 441
NlaIII CATG 6 cut(s) 163, 224, 268, 328, 531, 546
NlaIV GGNNCC 3 cut(s) 369, 376, 384
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 2 cut(s) 224, 328
PceI AGGCCT 1 cut(s) 299
PctI GAATGC 1 cut(s) 408
PfeI GAWTC 2 cut(s) 127, 451
PkrI GCNGC 5 cut(s) 36, 309, 330, 467, 574
PleI GAGTC 2 cut(s) 184, 198
PpsI GAGTC 2 cut(s) 184, 198
PshAI GACNNNNGTC 1 cut(s) 230
Psp6I CCWGG 4 cut(s) 270, 362, 370, 377
PspGI CCWGG 4 cut(s) 270, 362, 370, 377
PspN4I GGNNCC 3 cut(s) 369, 376, 384
PspPI GGNCC 1 cut(s) 375
PstI CTGCAG 1 cut(s) 577
PsuI RGATCY 2 cut(s) 382, 441
RsaI GTAC 2 cut(s) 152, 173
RsaNI GTAC 2 cut(s) 151, 172
SaqAI TTAA 3 cut(s) 186, 207, 312
SatI GCNGC 5 cut(s) 35, 308, 329, 466, 573
Sau3AI GATC 2 cut(s) 382, 441
Sau96I GGNCC 1 cut(s) 375
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 2 cut(s) 185, 199
ScrFI CCNGG 4 cut(s) 272, 364, 372, 379
SetI ASST 9 cut(s) 55, 69, 89, 99, 144, 207, 312, 342, 352
SfaNI GCATC 1 cut(s) 586
SfcI CTRYAG 2 cut(s) 495, 573
SseBI AGGCCT 1 cut(s) 299
SspMI CTAG 2 cut(s) 137, 387
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 4 cut(s) 270, 362, 370, 377
StyI CCWWGG 1 cut(s) 527
TaaI ACNGT 4 cut(s) 155, 229, 403, 505
TaqI TCGA 2 cut(s) 29, 478
TatI WGTACW 1 cut(s) 150
TfiI GAWTC 2 cut(s) 127, 451
Tru1I TTAA 3 cut(s) 186, 207, 312
Tru9I TTAA 3 cut(s) 186, 207, 312
TscAI CASTG 1 cut(s) 419
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 5 cut(s) 34, 307, 328, 465, 572
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 3 cut(s) 57, 176, 498
TspGWI ACGGA 1 cut(s) 591
TspRI CASTG 1 cut(s) 419
XceI RCATGY 2 cut(s) 224, 328
XmiI GTMKAC 1 cut(s) 144
XspI CTAG 2 cut(s) 137, 387
ZrmI AGTACT 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.