Rw6G023510

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002266
Physical Location & Seq
Forward (+)
46124781 .. 46127078
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G023510.1

Sequence Viewer

Length: 975 bp
ATGGGAAGTTCAAGTCAGAAACGCTCGAGATCAAGCTCAGTTATTTTCCAAATTGCCGGACGCAGTGTCAATCTTGCTCCACTTCCTGGATCGGTTGACAACAAAACTGCTGTTCAAGTTTCTACCATTGCAAAACCTGATCGTATGCGCTCCTTGCTTTTCACTGATTTGAATCAACAACCAGTGAAGCAATTTACTGCTACGAAATATTCCCTTGAGAATACCAGCATGTTGAGGGACCCGGCTAATGAGAGGGTTTTGCTTGACCTGCTTGGCCCTGTTGATGCCAGTATCATGCAGGCGTATACTGACAGTGATATAAGGCAGAACTTAGCCCACCATGCCCTTGCTTCCTCCCTCCACTTAGTCATGTGGCTGAGGAAAACTGGAGAGGTTTCGAGGCAGCGTGATGAAGCCATAGCTCGTGTTGCAGAGCTGGAGGAAAGAGTTAGGAGGTTTGAAAAGCTCTTTGTGAAACAGGAGGCTGAAAATGAGATTATGAGCCTAGAGGTCGACGAGTACTGTAACATGAAGGAGGAGTACGAGTCTAAGTTTAAGAGTCTATCAAATGAGGTTAACCAGCAGAAGCAGGTGACTGTGGTCTTGGAGAGTAAGTTCAAGGTTATGGAGGCCATGCACCAGGAGAAGGCTACTCGTCTGGAAAAGGCCTTGAAGCAGCTTAACGACGCCAACATGCTGCTTGCTGAGGTGAGTGAAGGTCTTGGTGTGGCCAGGGAACCAGGGGCCAGGGATCCTAGACATTTCTGGACGGTTTGCATTCACTGCAAAGTCCAGTATCAGTATATCAGGATCTATCAGAATCGCAGGCTTCGCTGCCCCAATTGTCGAAATGCTTTTCTTGCTATAGAAACTGTTTCATCTCCAAAATCCTATCCATGGAGTAGCCCTTCATGGGTGAAGCCTGAAGAAGATGGACAGGGTGCAGTCAAAGTGGAGGCTCAGGATGCTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

324

Amino Acids

36.86

Weight (kDa)

8.49

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_20 PF23551 255 - 289 1.6e-14 Zinc beta-ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 2 cut(s) 276, 580
AccB7I CCANNNNNTGG 1 cut(s) 86
AccI GTMKAC 2 cut(s) 305, 513
AclWI GGATC 4 cut(s) 97, 746, 759, 818
AcoI YGGCCR 1 cut(s) 729
AcuI CTGAAG 1 cut(s) 945
AcyI GRCGYC 1 cut(s) 687
AfaI GTAC 2 cut(s) 521, 542
AfiI CCNNNNNNNGG 4 cut(s) 86, 646, 897, 913
AgsI TTSAA 6 cut(s) 12, 116, 172, 461, 619, 673
AhdI GACNNNNNGTC 1 cut(s) 65
AjnI CCWGG 5 cut(s) 85, 639, 731, 739, 746
AloI GAACNNNNNNTCC 2 cut(s) 599, 631
AluBI AGCT 5 cut(s) 36, 422, 436, 466, 679
AluI AGCT 5 cut(s) 36, 422, 436, 466, 679
AlwI GGATC 4 cut(s) 97, 746, 759, 818
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 5 cut(s) 274, 630, 666, 729, 744
ApeKI GCWGC 4 cut(s) 403, 676, 697, 834
AspLEI GCGC 1 cut(s) 150
AspS9I GGNCC 3 cut(s) 238, 275, 744
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 3 cut(s) 604, 721, 928
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 1 cut(s) 238
BalI TGGCCA 1 cut(s) 731
BamHI GGATCC 1 cut(s) 751
BarI GAAGNNNNNNTAC 2 cut(s) 524, 556
BauI CACGAG 1 cut(s) 423
BbvCI CCTCAGC 2 cut(s) 377, 705
BbvI GCAGC 4 cut(s) 415, 684, 688, 821
BccI CCATC 1 cut(s) 926
BciT130I CCWGG 5 cut(s) 87, 641, 733, 741, 748
BcnI CCSGG 1 cut(s) 242
BfaI CTAG 2 cut(s) 506, 756
BfmI CTRYAG 1 cut(s) 864
BfuAI ACCTGC 2 cut(s) 276, 580
BisI GCNGC 4 cut(s) 404, 677, 698, 835
BlsI GCNGC 4 cut(s) 405, 678, 699, 836
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 6 cut(s) 87, 242, 641, 733, 741, 748
Bme18I GGWCC 1 cut(s) 238
BmeRI GACNNNNNGTC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 3 cut(s) 238, 275, 744
BmiI GGNNCC 5 cut(s) 239, 240, 738, 745, 753
BmrFI CCNGG 6 cut(s) 87, 242, 641, 733, 741, 748
BmsI GCATC 2 cut(s) 274, 955
BoxI GACNNNNGTC 1 cut(s) 599
BpmI CTGGAG 2 cut(s) 408, 458
Bpu10I CCTNAGC 3 cut(s) 377, 705, 960
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 242
BsaBI GATNNNNATC 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 687
BsaJI CCNNGG 4 cut(s) 732, 740, 747, 896
BsaXI ACNNNNNCTCC 4 cut(s) 530, 560, 599, 629
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 646, 897, 913
Bse1I ACTGG 4 cut(s) 182, 288, 391, 793
Bse3DI GCAATG 1 cut(s) 126
Bse8I GATNNNNATC 1 cut(s) 171
BseBI CCWGG 5 cut(s) 87, 641, 733, 741, 748
BseDI CCNNGG 4 cut(s) 732, 740, 747, 896
BseGI GGATG 1 cut(s) 970
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 4 cut(s) 86, 646, 897, 913
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 4 cut(s) 51, 368, 696, 974
BseNI ACTGG 4 cut(s) 182, 288, 391, 793
BseRI GAGGAG 1 cut(s) 551
BseXI GCAGC 4 cut(s) 415, 684, 688, 821
BsgI GTGCAG 1 cut(s) 963
BshFI GGCC 5 cut(s) 276, 632, 668, 731, 746
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 2 cut(s) 57, 242
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 4 cut(s) 86, 646, 897, 913
BsmFI GGGAC 1 cut(s) 251
BsmI GAATGC 1 cut(s) 777
BsnI GGCC 5 cut(s) 276, 632, 668, 731, 746
BsoBI CYCGRG 1 cut(s) 25
Bsp143I GATC 5 cut(s) 29, 89, 139, 751, 810
Bsp19I CCATGG 1 cut(s) 896
BspANI GGCC 5 cut(s) 276, 632, 668, 731, 746
BspCNI CTCAG 4 cut(s) 50, 369, 697, 973
BspLI GGNNCC 5 cut(s) 239, 240, 738, 745, 753
BspMI ACCTGC 2 cut(s) 276, 580
BspPI GGATC 4 cut(s) 97, 746, 759, 818
BsrDI GCAATG 1 cut(s) 126
BsrI ACTGG 4 cut(s) 182, 288, 391, 793
BssECI CCNNGG 4 cut(s) 732, 740, 747, 896
BssMI GATC 5 cut(s) 29, 89, 139, 751, 810
BssNAI GTATAC 1 cut(s) 306
BssNI GRCGYC 1 cut(s) 687
BssSI CACGAG 1 cut(s) 423
BssT1I CCWWGG 1 cut(s) 896
Bst1107I GTATAC 1 cut(s) 306
Bst2BI CACGAG 1 cut(s) 423
Bst2UI CCWGG 5 cut(s) 87, 641, 733, 741, 748
Bst4CI ACNGT 5 cut(s) 314, 524, 598, 772, 874
BstACI GRCGYC 1 cut(s) 687
BstAPI GCANNNNNTGC 1 cut(s) 783
BstC8I GCNNGC 3 cut(s) 300, 702, 827
BstDEI CTNAG 7 cut(s) 37, 331, 364, 377, 549, 705, 960
BstDSI CCRYGG 1 cut(s) 896
BstF5I GGATG 1 cut(s) 970
BstHHI GCGC 1 cut(s) 150
BstKTI GATC 5 cut(s) 32, 92, 142, 754, 813
BstMBI GATC 5 cut(s) 29, 89, 139, 751, 810
BstMWI GCNNNNNNNGC 8 cut(s) 154, 268, 341, 428, 783, 831, 860, 965
BstNI CCWGG 5 cut(s) 87, 641, 733, 741, 748
BstNSI RCATGY 2 cut(s) 232, 697
BstPAI GACNNNNGTC 1 cut(s) 599
BstSCI CCNGG 6 cut(s) 85, 240, 639, 731, 739, 746
BstSFI CTRYAG 1 cut(s) 864
BstV1I GCAGC 4 cut(s) 415, 684, 688, 821
BstX2I RGATCY 2 cut(s) 751, 810
BstYI RGATCY 2 cut(s) 751, 810
BstZ17I GTATAC 1 cut(s) 306
BsuRI GGCC 5 cut(s) 276, 632, 668, 731, 746
BtgI CCRYGG 1 cut(s) 896
BtsCI GGATG 1 cut(s) 970
BtsI GCAGTG 2 cut(s) 70, 781
BtsIMutI CAGTG 5 cut(s) 70, 162, 189, 319, 781
BveI ACCTGC 2 cut(s) 276, 580
Cac8I GCNNGC 3 cut(s) 300, 702, 827
CfoI GCGC 1 cut(s) 150
Cfr13I GGNCC 3 cut(s) 238, 275, 744
CseI GACGC 2 cut(s) 69, 695
Csp6I GTAC 2 cut(s) 520, 541
CviAII CATG 9 cut(s) 229, 295, 341, 370, 529, 634, 694, 897, 912
CviQI GTAC 2 cut(s) 520, 541
DdeI CTNAG 7 cut(s) 37, 331, 364, 377, 549, 705, 960
DpnI GATC 5 cut(s) 31, 91, 141, 753, 812
DpnII GATC 5 cut(s) 29, 89, 139, 751, 810
DriI GACNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 1 cut(s) 729
Eam1105I GACNNNNNGTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 896
Eco147I AGGCCT 1 cut(s) 668
Eco47I GGWCC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 945
Eco88I CYCGRG 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 238
EcoRII CCWGG 5 cut(s) 85, 639, 731, 739, 746
EcoT14I CCWWGG 1 cut(s) 896
ErhI CCWWGG 1 cut(s) 896
FaeI CATG 9 cut(s) 232, 298, 344, 373, 532, 637, 697, 900, 915
FaqI GGGAC 1 cut(s) 251
FatI CATG 9 cut(s) 228, 294, 340, 369, 528, 633, 693, 896, 911
FblI GTMKAC 2 cut(s) 305, 513
Fnu4HI GCNGC 4 cut(s) 404, 677, 698, 835
Fsp4HI GCNGC 4 cut(s) 404, 677, 698, 835
FspBI CTAG 2 cut(s) 506, 756
GlaI GCGC 1 cut(s) 149
GluI GCNGC 4 cut(s) 404, 677, 698, 835
GsuI CTGGAG 2 cut(s) 408, 458
HaeIII GGCC 5 cut(s) 276, 632, 668, 731, 746
HapII CCGG 2 cut(s) 57, 242
HgaI GACGC 2 cut(s) 69, 695
HhaI GCGC 1 cut(s) 150
Hin1I GRCGYC 1 cut(s) 687
Hin1II CATG 9 cut(s) 232, 298, 344, 373, 532, 637, 697, 900, 915
Hin6I GCGC 1 cut(s) 148
HinP1I GCGC 1 cut(s) 148
HincII GTYRAC 3 cut(s) 97, 514, 577
HindII GTYRAC 3 cut(s) 97, 514, 577
HinfI GANTC 4 cut(s) 172, 545, 559, 820
HpaI GTTAAC 1 cut(s) 577
HpaII CCGG 2 cut(s) 57, 242
HphI GGTGA 3 cut(s) 604, 721, 928
Hpy166II GTNNAC 4 cut(s) 97, 306, 514, 577
Hpy188I TCNGA 2 cut(s) 18, 819
Hpy188III TCNNGA 5 cut(s) 27, 659, 766, 808, 962
Hpy8I GTNNAC 4 cut(s) 97, 306, 514, 577
Hpy99I CGWCG 2 cut(s) 518, 689
HpyAV CCTTC 4 cut(s) 526, 640, 710, 918
HpyCH4III ACNGT 5 cut(s) 314, 524, 598, 772, 874
HpyCH4V TGCA 7 cut(s) 131, 298, 431, 637, 777, 786, 944
HpyF10VI GCNNNNNNNGC 8 cut(s) 154, 268, 341, 428, 783, 831, 860, 965
HpyF3I CTNAG 7 cut(s) 37, 331, 364, 377, 549, 705, 960
Hsp92I GRCGYC 1 cut(s) 687
Hsp92II CATG 9 cut(s) 232, 298, 344, 373, 532, 637, 697, 900, 915
HspAI GCGC 1 cut(s) 148
KflI GGGWCCC 1 cut(s) 238
KspAI GTTAAC 1 cut(s) 577
Kzo9I GATC 5 cut(s) 29, 89, 139, 751, 810
LmnI GCTCC 3 cut(s) 82, 155, 973
Lsp1109I GCAGC 4 cut(s) 415, 684, 688, 821
LweI GCATC 2 cut(s) 274, 955
MaeI CTAG 2 cut(s) 506, 756
MaeIII GTNAC 2 cut(s) 524, 592
MalI GATC 5 cut(s) 31, 91, 141, 753, 812
MboI GATC 5 cut(s) 29, 89, 139, 751, 810
MboII GAAGA 2 cut(s) 938, 941
MfeI CAATTG 1 cut(s) 841
MflI RGATCY 2 cut(s) 751, 810
MlsI TGGCCA 1 cut(s) 731
MluCI AATT 3 cut(s) 51, 191, 841
MluNI TGGCCA 1 cut(s) 731
MlyI GAGTC 2 cut(s) 554, 568
Mox20I TGGCCA 1 cut(s) 731
MscI TGGCCA 1 cut(s) 731
MseI TTAA 3 cut(s) 555, 576, 681
Msp20I TGGCCA 1 cut(s) 731
MspI CCGG 2 cut(s) 57, 242
MspR9I CCNGG 6 cut(s) 87, 242, 641, 733, 741, 748
MunI CAATTG 1 cut(s) 841
Mva1269I GAATGC 1 cut(s) 777
MvaI CCWGG 5 cut(s) 87, 641, 733, 741, 748
MwoI GCNNNNNNNGC 8 cut(s) 154, 268, 341, 428, 783, 831, 860, 965
NciI CCSGG 1 cut(s) 242
NcoI CCATGG 1 cut(s) 896
NdeII GATC 5 cut(s) 29, 89, 139, 751, 810
NlaIII CATG 9 cut(s) 232, 298, 344, 373, 532, 637, 697, 900, 915
NlaIV GGNNCC 5 cut(s) 239, 240, 738, 745, 753
NmuCI GTSAC 1 cut(s) 592
NspI RCATGY 2 cut(s) 232, 697
PaeR7I CTCGAG 1 cut(s) 25
PaqCI CACCTGC 1 cut(s) 580
PceI AGGCCT 1 cut(s) 668
PctI GAATGC 1 cut(s) 777
PfeI GAWTC 2 cut(s) 172, 820
PflMI CCANNNNNTGG 1 cut(s) 86
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 4 cut(s) 405, 678, 699, 836
PleI GAGTC 2 cut(s) 553, 567
PpsI GAGTC 2 cut(s) 553, 567
PpuMI RGGWCCY 1 cut(s) 238
PshAI GACNNNNGTC 1 cut(s) 599
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 5 cut(s) 85, 639, 731, 739, 746
PspGI CCWGG 5 cut(s) 85, 639, 731, 739, 746
PspN4I GGNNCC 5 cut(s) 239, 240, 738, 745, 753
PspPI GGNCC 3 cut(s) 238, 275, 744
PspPPI RGGWCCY 1 cut(s) 238
PsuI RGATCY 2 cut(s) 751, 810
RsaI GTAC 2 cut(s) 521, 542
RsaNI GTAC 2 cut(s) 520, 541
SalI GTCGAC 1 cut(s) 512
SaqAI TTAA 3 cut(s) 555, 576, 681
SatI GCNGC 4 cut(s) 404, 677, 698, 835
Sau3AI GATC 5 cut(s) 29, 89, 139, 751, 810
Sau96I GGNCC 3 cut(s) 238, 275, 744
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 2 cut(s) 554, 568
ScrFI CCNGG 6 cut(s) 87, 242, 641, 733, 741, 748
SfaNI GCATC 2 cut(s) 274, 955
SfcI CTRYAG 1 cut(s) 864
Sfr274I CTCGAG 1 cut(s) 25
SinI GGWCC 1 cut(s) 238
SlaI CTCGAG 1 cut(s) 25
SmlI CTYRAG 2 cut(s) 25, 215
SmoI CTYRAG 2 cut(s) 25, 215
Sse9I AATT 3 cut(s) 51, 191, 841
SseBI AGGCCT 1 cut(s) 668
SspI AATATT 1 cut(s) 209
SspMI CTAG 2 cut(s) 506, 756
StuI AGGCCT 1 cut(s) 668
StyD4I CCNGG 6 cut(s) 85, 240, 639, 731, 739, 746
StyI CCWWGG 1 cut(s) 896
TaaI ACNGT 5 cut(s) 314, 524, 598, 772, 874
TaqI TCGA 4 cut(s) 26, 398, 513, 847
TasI AATT 3 cut(s) 51, 191, 841
TatI WGTACW 1 cut(s) 519
TfiI GAWTC 2 cut(s) 172, 820
Tru1I TTAA 3 cut(s) 555, 576, 681
Tru9I TTAA 3 cut(s) 555, 576, 681
TscAI CASTG 5 cut(s) 70, 169, 189, 319, 788
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 4 cut(s) 403, 676, 697, 834
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 4 cut(s) 426, 545, 867, 900
TspGWI ACGGA 1 cut(s) 960
TspRI CASTG 5 cut(s) 70, 169, 189, 319, 788
Van91I CCANNNNNTGG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 238
XceI RCATGY 2 cut(s) 232, 697
XhoI CTCGAG 1 cut(s) 25
XmiI GTMKAC 2 cut(s) 305, 513
XspI CTAG 2 cut(s) 506, 756
ZrmI AGTACT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.