Rroxscaffold_7G00184970

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002266
Physical Location & Seq
Reverse (-)
24253814 .. 24256853
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00184970.1

Sequence Viewer

Length: 1065 bp
ATGGCTGAGCCACCTCGTTCCTCTGTTTTCTTCGTCAAAAGCTTGTTAATCATGTTGAAAGCACAGAGTCCTCTCATTGATTGGGATGAGTTCATATTTGGAGTCTTTGATGTGTTTTGGTATGCATTTGATTTTAGACGCAGTGTCAATCTTGCTCCACTTCCTGGATCGGTTGACAACAAAACTGCTGTTCAAGTTTCTACCACTGCAAAATCTGATCATATGCGCCCCTTGATTTTCACTGATTTGAATCAACAACCAGTGAAGCAATTTACTGCTACAAAATATTCCCTTGAGAATACCAGCATGTTGAGGGACCCGGCTACTGCTGAGAGGGTTTTGCTTGACCTGCTTGGCCCTGTTGATGCCAGCATCATGCAGGCGTATACTGACAGTGATATAAGGCAGAACTTAGCCCACCATGCCTTTGCTTCCTCCCTCCACTTAGCCATGTGGCTGAGGAAAACTGGAGATGTTTCGAGTCAGCGCGATGAAGCCATAGCTCGTGTTGCAGAGCTGGAGGAAAGAGTTAGGAGGTTTGAAAAGCTCTTTCTGAAACAGGAGGCTGAAAATGAGATTCTGAGCCTAGAGGTCGACGAGTACTGTAACATGGAGGAGGAGTACGAGTCTAAGATTAAGAGTCTATCAAATGAGGTTAACCAGCAGAAGCGGGTGACTGTGGTCTTGGAGAGTAAGTTCAAGGATATGGAGGCCATGCACCAGGAGAAGGCTACTCGTCTGGAAAAGGCCTTGAAGCAGCTTAACGACGCCAACATGCTGCTTGCTGAGGTGAGTGAAGGTCTTGGTGTGGCCAGGGAACCAGGGGCCAGGGATCCTAGACATTTCTGGACGGTTTGCATTCACTGCAAAGTCCAGTATCAGTATATCAAGATCTATCAGAATCGCAGGCTTCGCTGCCCCAATTGTCGAAATGCTTTTCTTGCTATAGAAACTGTTTCATCTCCAAAGTCCTATCCATGGAGTAGCCCTTCATGGGTGAAGCCTGAGGAAGATGGACAGGGTGCAGTCAAAGTGGAGGCTCAGGATGCTCGGTTAGTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

354

Amino Acids

40.51

Weight (kDa)

5.91

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_20 PF23551 282 - 316 7.6e-14 Zinc beta-ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 357
AccB7I CCANNNNNTGG 1 cut(s) 164
AccI GTMKAC 2 cut(s) 386, 594
AccII CGCG 1 cut(s) 489
AciI CCGC 1 cut(s) 670
AclWI GGATC 3 cut(s) 175, 827, 840
AcoI YGGCCR 1 cut(s) 810
AcyI GRCGYC 1 cut(s) 768
AfaI GTAC 2 cut(s) 602, 623
AfiI CCNNNNNNNGG 4 cut(s) 164, 727, 978, 994
AgsI TTSAA 6 cut(s) 58, 194, 250, 542, 700, 754
AhdI GACNNNNNGTC 1 cut(s) 143
AjnI CCWGG 5 cut(s) 163, 720, 812, 820, 827
AloI GAACNNNNNNTCC 2 cut(s) 680, 712
AluBI AGCT 5 cut(s) 42, 503, 517, 547, 760
AluI AGCT 5 cut(s) 42, 503, 517, 547, 760
AlwI GGATC 3 cut(s) 175, 827, 840
AoxI GGCC 5 cut(s) 355, 711, 747, 810, 825
ApeKI GCWGC 3 cut(s) 757, 778, 915
AspLEI GCGC 2 cut(s) 228, 489
AspS9I GGNCC 3 cut(s) 316, 356, 825
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 3 cut(s) 685, 802, 1009
AvaII GGWCC 1 cut(s) 316
AxyI CCTNAGG 1 cut(s) 1005
BalI TGGCCA 1 cut(s) 812
BamHI GGATCC 1 cut(s) 832
BauI CACGAG 1 cut(s) 504
BbvCI CCTCAGC 2 cut(s) 458, 786
BbvI GCAGC 3 cut(s) 765, 769, 902
BccI CCATC 1 cut(s) 1007
BciT130I CCWGG 5 cut(s) 165, 722, 814, 822, 829
BclI TGATCA 1 cut(s) 217
BcnI CCSGG 1 cut(s) 320
BfaI CTAG 2 cut(s) 587, 837
BfmI CTRYAG 1 cut(s) 945
BfuAI ACCTGC 1 cut(s) 357
BglII AGATCT 1 cut(s) 891
BisI GCNGC 3 cut(s) 758, 779, 916
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 3 cut(s) 759, 780, 917
BmcAI AGTACT 1 cut(s) 602
Bme1390I CCNGG 6 cut(s) 165, 320, 722, 814, 822, 829
Bme18I GGWCC 1 cut(s) 316
BmeRI GACNNNNNGTC 1 cut(s) 143
BmgT120I GGNCC 3 cut(s) 316, 356, 825
BmiI GGNNCC 5 cut(s) 317, 318, 819, 826, 834
BmrFI CCNGG 6 cut(s) 165, 320, 722, 814, 822, 829
BmsI GCATC 3 cut(s) 355, 381, 1036
BoxI GACNNNNGTC 1 cut(s) 680
BpmI CTGGAG 2 cut(s) 489, 539
Bpu10I CCTNAGC 3 cut(s) 458, 786, 1041
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 249
BsaHI GRCGYC 1 cut(s) 768
BsaJI CCNNGG 4 cut(s) 813, 821, 828, 977
BsaXI ACNNNNNCTCC 6 cut(s) 605, 611, 635, 641, 680, 710
Bsc4I CCNNNNNNNGG 4 cut(s) 164, 727, 978, 994
Bse1I ACTGG 3 cut(s) 260, 472, 874
Bse21I CCTNAGG 1 cut(s) 1005
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 5 cut(s) 165, 722, 814, 822, 829
BseDI CCNNGG 4 cut(s) 813, 821, 828, 977
BseGI GGATG 2 cut(s) 91, 1051
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 4 cut(s) 164, 727, 978, 994
BseMII CTCAG 6 cut(s) 321, 449, 572, 777, 996, 1055
BseNI ACTGG 3 cut(s) 260, 472, 874
BseRI GAGGAG 2 cut(s) 629, 632
BseXI GCAGC 3 cut(s) 765, 769, 902
BsgI GTGCAG 1 cut(s) 1044
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 5 cut(s) 357, 713, 749, 812, 827
BsiSI CCGG 1 cut(s) 320
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 4 cut(s) 164, 727, 978, 994
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 858
BsnI GGCC 5 cut(s) 357, 713, 749, 812, 827
Bsp143I GATC 4 cut(s) 167, 217, 832, 891
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 977
BspACI CCGC 1 cut(s) 670
BspANI GGCC 5 cut(s) 357, 713, 749, 812, 827
BspCNI CTCAG 6 cut(s) 322, 450, 573, 778, 997, 1054
BspFNI CGCG 1 cut(s) 489
BspLI GGNNCC 5 cut(s) 317, 318, 819, 826, 834
BspMI ACCTGC 1 cut(s) 357
BspPI GGATC 3 cut(s) 175, 827, 840
BsrI ACTGG 3 cut(s) 260, 472, 874
BssECI CCNNGG 4 cut(s) 813, 821, 828, 977
BssMI GATC 4 cut(s) 167, 217, 832, 891
BssNAI GTATAC 1 cut(s) 387
BssNI GRCGYC 1 cut(s) 768
BssSI CACGAG 1 cut(s) 504
BssT1I CCWWGG 1 cut(s) 977
Bst1107I GTATAC 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 5 cut(s) 165, 722, 814, 822, 829
Bst4CI ACNGT 5 cut(s) 395, 605, 679, 853, 955
BstACI GRCGYC 1 cut(s) 768
BstAPI GCANNNNNTGC 1 cut(s) 864
BstC8I GCNNGC 4 cut(s) 370, 381, 783, 908
BstDSI CCRYGG 1 cut(s) 977
BstF5I GGATG 2 cut(s) 91, 1051
BstFNI CGCG 1 cut(s) 489
BstHHI GCGC 2 cut(s) 228, 489
BstKTI GATC 4 cut(s) 170, 220, 835, 894
BstMBI GATC 4 cut(s) 167, 217, 832, 891
BstMWI GCNNNNNNNGC 7 cut(s) 349, 422, 509, 864, 912, 941, 1046
BstNI CCWGG 5 cut(s) 165, 722, 814, 822, 829
BstNSI RCATGY 2 cut(s) 310, 778
BstPAI GACNNNNGTC 1 cut(s) 680
BstSCI CCNGG 6 cut(s) 163, 318, 720, 812, 820, 827
BstSFI CTRYAG 1 cut(s) 945
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 3 cut(s) 765, 769, 902
BstX2I RGATCY 2 cut(s) 832, 891
BstYI RGATCY 2 cut(s) 832, 891
BstZ17I GTATAC 1 cut(s) 387
Bsu36I CCTNAGG 1 cut(s) 1005
BsuRI GGCC 5 cut(s) 357, 713, 749, 812, 827
BtgI CCRYGG 1 cut(s) 977
BtgZI GCGATG 1 cut(s) 504
BtsCI GGATG 2 cut(s) 91, 1051
BtsI GCAGTG 3 cut(s) 148, 204, 862
BtsIMutI CAGTG 6 cut(s) 148, 204, 240, 267, 400, 862
BveI ACCTGC 1 cut(s) 357
Cac8I GCNNGC 4 cut(s) 370, 381, 783, 908
CfoI GCGC 2 cut(s) 228, 489
Cfr13I GGNCC 3 cut(s) 316, 356, 825
CseI GACGC 2 cut(s) 147, 776
Csp6I GTAC 2 cut(s) 601, 622
CviQI GTAC 2 cut(s) 601, 622
DpnI GATC 4 cut(s) 169, 219, 834, 893
DpnII GATC 4 cut(s) 167, 217, 832, 891
DriI GACNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 810
Eam1105I GACNNNNNGTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 977
Eco147I AGGCCT 1 cut(s) 749
Eco47I GGWCC 1 cut(s) 316
Eco81I CCTNAGG 1 cut(s) 1005
EcoO109I RGGNCCY 1 cut(s) 316
EcoRII CCWGG 5 cut(s) 163, 720, 812, 820, 827
EcoT14I CCWWGG 1 cut(s) 977
EcoT22I ATGCAT 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 977
FaqI GGGAC 1 cut(s) 329
FauI CCCGC 1 cut(s) 663
FauNDI CATATG 1 cut(s) 222
FbaI TGATCA 1 cut(s) 217
FblI GTMKAC 2 cut(s) 386, 594
Fnu4HI GCNGC 3 cut(s) 758, 779, 916
FokI GGATG 2 cut(s) 98, 1058
Fsp4HI GCNGC 3 cut(s) 758, 779, 916
FspBI CTAG 2 cut(s) 587, 837
GlaI GCGC 2 cut(s) 227, 488
GluI GCNGC 3 cut(s) 758, 779, 916
GsuI CTGGAG 2 cut(s) 489, 539
HaeIII GGCC 5 cut(s) 357, 713, 749, 812, 827
HapII CCGG 1 cut(s) 320
HgaI GACGC 2 cut(s) 147, 776
HhaI GCGC 2 cut(s) 228, 489
Hin1I GRCGYC 1 cut(s) 768
Hin6I GCGC 2 cut(s) 226, 487
HinP1I GCGC 2 cut(s) 226, 487
HincII GTYRAC 3 cut(s) 175, 595, 658
HindII GTYRAC 3 cut(s) 175, 595, 658
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 8 cut(s) 67, 102, 250, 481, 577, 626, 640, 901
HpaI GTTAAC 1 cut(s) 658
HpaII CCGG 1 cut(s) 320
HphI GGTGA 3 cut(s) 685, 802, 1009
Hpy166II GTNNAC 4 cut(s) 175, 387, 595, 658
Hpy188I TCNGA 4 cut(s) 217, 555, 582, 900
Hpy188III TCNNGA 4 cut(s) 740, 847, 889, 1043
Hpy8I GTNNAC 4 cut(s) 175, 387, 595, 658
Hpy99I CGWCG 2 cut(s) 599, 770
HpyAV CCTTC 3 cut(s) 721, 791, 999
HpyCH4III ACNGT 5 cut(s) 395, 605, 679, 853, 955
HpyCH4V TGCA 8 cut(s) 125, 209, 379, 512, 718, 858, 867, 1025
HpyF10VI GCNNNNNNNGC 7 cut(s) 349, 422, 509, 864, 912, 941, 1046
Hsp92I GRCGYC 1 cut(s) 768
HspAI GCGC 2 cut(s) 226, 487
KflI GGGWCCC 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 217
KspAI GTTAAC 1 cut(s) 658
Kzo9I GATC 4 cut(s) 167, 217, 832, 891
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 3 cut(s) 765, 769, 902
LweI GCATC 3 cut(s) 355, 381, 1036
MaeI CTAG 2 cut(s) 587, 837
MaeIII GTNAC 2 cut(s) 605, 673
MalI GATC 4 cut(s) 169, 219, 834, 893
MboI GATC 4 cut(s) 167, 217, 832, 891
MboII GAAGA 2 cut(s) 22, 1022
MfeI CAATTG 1 cut(s) 922
MflI RGATCY 2 cut(s) 832, 891
MlsI TGGCCA 1 cut(s) 812
MluCI AATT 2 cut(s) 269, 922
MluNI TGGCCA 1 cut(s) 812
MlyI GAGTC 5 cut(s) 76, 111, 490, 635, 649
Mox20I TGGCCA 1 cut(s) 812
Mph1103I ATGCAT 1 cut(s) 127
MscI TGGCCA 1 cut(s) 812
MseI TTAA 5 cut(s) 47, 636, 657, 762, 1063
Msp20I TGGCCA 1 cut(s) 812
MspI CCGG 1 cut(s) 320
MspR9I CCNGG 6 cut(s) 165, 320, 722, 814, 822, 829
MunI CAATTG 1 cut(s) 922
Mva1269I GAATGC 1 cut(s) 858
MvaI CCWGG 5 cut(s) 165, 722, 814, 822, 829
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 7 cut(s) 349, 422, 509, 864, 912, 941, 1046
NciI CCSGG 1 cut(s) 320
NcoI CCATGG 1 cut(s) 977
NdeI CATATG 1 cut(s) 222
NdeII GATC 4 cut(s) 167, 217, 832, 891
NlaIV GGNNCC 5 cut(s) 317, 318, 819, 826, 834
NmuCI GTSAC 1 cut(s) 673
NsiI ATGCAT 1 cut(s) 127
NspI RCATGY 2 cut(s) 310, 778
PceI AGGCCT 1 cut(s) 749
PctI GAATGC 1 cut(s) 858
PfeI GAWTC 3 cut(s) 250, 577, 901
PflMI CCANNNNNTGG 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 163
PkrI GCNGC 3 cut(s) 759, 780, 917
PleI GAGTC 5 cut(s) 75, 110, 489, 634, 648
PpsI GAGTC 5 cut(s) 75, 110, 489, 634, 648
PpuMI RGGWCCY 1 cut(s) 316
PshAI GACNNNNGTC 1 cut(s) 680
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 5 cut(s) 163, 720, 812, 820, 827
PspGI CCWGG 5 cut(s) 163, 720, 812, 820, 827
PspN4I GGNNCC 5 cut(s) 317, 318, 819, 826, 834
PspPI GGNCC 3 cut(s) 316, 356, 825
PspPPI RGGWCCY 1 cut(s) 316
PsuI RGATCY 2 cut(s) 832, 891
RsaI GTAC 2 cut(s) 602, 623
RsaNI GTAC 2 cut(s) 601, 622
SalI GTCGAC 1 cut(s) 593
SaqAI TTAA 5 cut(s) 47, 636, 657, 762, 1063
SatI GCNGC 3 cut(s) 758, 779, 916
Sau3AI GATC 4 cut(s) 167, 217, 832, 891
Sau96I GGNCC 3 cut(s) 316, 356, 825
ScaI AGTACT 1 cut(s) 602
SchI GAGTC 5 cut(s) 76, 111, 490, 635, 649
ScrFI CCNGG 6 cut(s) 165, 320, 722, 814, 822, 829
SfaNI GCATC 3 cut(s) 355, 381, 1036
SfcI CTRYAG 1 cut(s) 945
SinI GGWCC 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 2 cut(s) 269, 922
SseBI AGGCCT 1 cut(s) 749
SsiI CCGC 1 cut(s) 670
SspI AATATT 1 cut(s) 287
SspMI CTAG 2 cut(s) 587, 837
StuI AGGCCT 1 cut(s) 749
StyD4I CCNGG 6 cut(s) 163, 318, 720, 812, 820, 827
StyI CCWWGG 1 cut(s) 977
TaaI ACNGT 5 cut(s) 395, 605, 679, 853, 955
TaqI TCGA 3 cut(s) 479, 594, 928
TasI AATT 2 cut(s) 269, 922
TatI WGTACW 1 cut(s) 600
TfiI GAWTC 3 cut(s) 250, 577, 901
Tru1I TTAA 5 cut(s) 47, 636, 657, 762, 1063
Tru9I TTAA 5 cut(s) 47, 636, 657, 762, 1063
TscAI CASTG 6 cut(s) 148, 211, 247, 267, 400, 869
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 3 cut(s) 757, 778, 915
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 4 cut(s) 82, 507, 948, 981
TspRI CASTG 6 cut(s) 148, 211, 247, 267, 400, 869
Van91I CCANNNNNTGG 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 316
XceI RCATGY 2 cut(s) 310, 778
XmiI GTMKAC 2 cut(s) 386, 594
XspI CTAG 2 cut(s) 587, 837
ZrmI AGTACT 1 cut(s) 602
Zsp2I ATGCAT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.